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CN116267266A - A kind of multiflora Gou catechu layering treatment and multiplication method - Google Patents

A kind of multiflora Gou catechu layering treatment and multiplication method Download PDF

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Publication number
CN116267266A
CN116267266A CN202211703847.7A CN202211703847A CN116267266A CN 116267266 A CN116267266 A CN 116267266A CN 202211703847 A CN202211703847 A CN 202211703847A CN 116267266 A CN116267266 A CN 116267266A
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multiflora
layering
treatment
girdling
epicatechin
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赵恬
张锦华
王胤晨
袁扬
张定红
邓梦青
梁峰豪
高娅妮
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Guizhou Oil Research Institute Guizhou Flavor Research Institute
Guizhou Institute Of Animal Husbandry And Veterinary Medicine
Shaanxi Academy of Forestry
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Guizhou Oil Research Institute Guizhou Flavor Research Institute
Guizhou Institute Of Animal Husbandry And Veterinary Medicine
Shaanxi Academy of Forestry
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/20Layering
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A01G24/15Calcined rock, e.g. perlite, vermiculite or clay aggregates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G3/00Cutting implements specially adapted for horticultural purposes; Delimbing standing trees
    • A01G3/08Other tools for pruning, branching or delimbing standing trees
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Soil Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a multi-flower epicatechin layering processing and expanding propagation method, which relates to the technical field of plant planting, in particular to a multi-flower epicatechin layering processing and expanding propagation method, comprising the following steps: s1, proportioning a substrate, namely, selecting perlite, turf and vermiculite as the substrate for the strip treatment of the multi-flower epicatechin, and mixing the perlite, the turf and the vermiculite with water to prepare the substrate for the strip treatment of the multi-flower epicatechin. According to the multi-flower epicatechin layering processing propagation method, perlite, turf and vermiculite are selected as matrixes for multi-flower epicatechin layering processing, NAA naphthalene acetic acid is selected as a growth regulator for multi-flower epicatechin layering processing propagation, NAA processing concentration is 400mg/L, and girdling processing means with girdling width of 8mm is selected, so that the influence test of rooting survival rate of multi-flower epicatechin propagation can be realized; the obtained layering has higher rooting rate (more than 90 percent), and the rooting rate is high, thereby being suitable for seedling propagation.

Description

一种多花勾儿茶压条处理扩繁方法A kind of multiflora Gou catechu layering treatment and multiplication method

技术领域technical field

本发明涉及植物种植技术领域,具体为一种多花勾儿茶压条处理扩繁方法。The invention relates to the technical field of plant planting, in particular to a multiflora catechu multiflora layering treatment and multiplication method.

背景技术Background technique

多花勾儿茶(学名:Berchemiafloribunda(Wall.)Brongn.)是鼠李科勾儿茶属植物,藤状或直立灌木;幼枝黄绿色,光滑无毛。叶纸质,上部叶较小,卵形或卵状椭圆形至与卵状披针形。花多数,通常数个簇生排成顶生宽聚伞圆锥花序,或下部兼腋生聚伞总状花序。核果圆柱状椭圆形,长7-10毫米,直径4-5毫米,果梗长2-3毫米,无毛。花期7-10月,果期翌时年4-7月。多花勾儿茶根入药,有祛风除湿,散瘀消肿、止痛之功效;农民常用枝作牛鼻圈;嫩叶可代茶。多花勾儿茶是优良的观果树种,萌芽能力强,耐修剪。其枝叶光亮,柔枝轻盈,颇具特色。金秋时节,硕果累累,红艳缤纷,是优良的观果植物。公园点缀、花坛造景等均可使用,在城市森林景观营造中可作灌木层配置。此外,其花期长、花量大、流蜜量丰富,可作为优良的辅助蜜源植物。Berchemia floribunda (scientific name: Berchemia floribunda (Wall.) Brongn.) is a plant of the genus Berchemia floribunda in the family Rhamnaceae, a vine-like or erect shrub; young branches are yellow-green, smooth and hairless. Leaves papery, upper leaves smaller, ovate or ovate-elliptic to ovate-lanceolate. Flowers numerous, usually several clustered in terminal broad thyrses, or lower part and axillary cymes. Drupe cylindrical-elliptic, 7-10 mm long, 4-5 mm in diameter, fruiting stem 2-3 mm long, glabrous. The flowering period is July-October, and the fruit period is April-July next year. The root of Gouta multiflora is used as medicine, which has the effects of dispelling wind and dampness, dispelling blood stasis, reducing swelling, and relieving pain; farmers often use the branches as ox nose rings; the young leaves can replace tea. Goucatecha multiflora is an excellent fruit-watching tree species, with strong germination ability and pruning resistance. Its branches and leaves are bright, and the soft branches are light and light, which is quite distinctive. In the golden autumn season, there are many fruits, red and colorful, and it is an excellent fruit viewing plant. It can be used for park decoration, flower bed landscaping, etc., and can be used as shrub layer configuration in urban forest landscape construction. In addition, it has a long flowering period, a large amount of flowers, and a rich nectar flow, so it can be used as an excellent auxiliary nectar source plant.

高枝压条是指在半木质化的枝条上进行环状剥皮或刻伤、缚缢后,用湿润的营养土包裹伤口处待其生根,生根后剪离母株,移栽即成新植株。高枝压条具有生根迅速、成活率高、保持母株优良性状、生理年龄大、投产早等优点,是一种适应范围较广的繁殖方法,生产中普遍用于龙眼、荔枝、柑橘、月季、茶花等植物的繁殖。由于多花勾儿茶多处于野生状态,对微环境和小气候要求较高,具有生境特异性和种子败育及种子休眠现象,自然环境下繁殖率低、更新困难,加上人为干扰使其生境破坏严重,导致野生植株分布大幅减少。关于多花勾儿茶的常规人工繁殖迄今尚无报道和研究,因此,高效繁育技术的研究对其生境的修复与重建、种质繁育及园林开发推广具有重要意义。High-branch layering refers to ring-peeling, cutting, and hanging semi-lignified branches, wrapping the wound with moist nutrient soil and waiting for it to take root. After rooting, cut off the mother plant and transplant it to become a new plant. High-branch layering has the advantages of rapid rooting, high survival rate, good traits of the mother plant, long physiological age, and early production. It is a breeding method with a wide range of adaptation. It is widely used in longan, litchi, citrus, rose, and camellia in production. and other plant reproduction. Since G. multiflora is mostly in the wild state, it has high requirements on the microenvironment and microclimate, and has habitat specificity, seed abortion and seed dormancy. The reproduction rate is low in the natural environment, and it is difficult to regenerate. The damage was severe, resulting in a significant reduction in the distribution of wild plants. So far, there has been no report and research on the conventional artificial propagation of G. multiflora. Therefore, the research on efficient breeding technology is of great significance to the restoration and reconstruction of its habitat, germplasm breeding and garden development and promotion.

发明内容Contents of the invention

针对现有技术的不足,本发明提供了一种多花勾儿茶压条处理扩繁方法,解决了上述背景技术中提出的多花勾儿茶发芽及繁殖非常困难的问题。Aiming at the deficiencies in the prior art, the present invention provides a method for processing and multiplying Catechus multiflora by layering, which solves the problem of very difficult germination and propagation of Catechus multiflora proposed in the above-mentioned background technology.

为实现以上目的,本发明通过以下技术方案予以实现:一种多花勾儿茶压条处理扩繁方法,其方法包括以下步骤:In order to achieve the above object, the present invention is achieved through the following technical solutions: a method for multiflora catechu multiflora layering processing and multiplication, the method comprising the following steps:

S1、基质配比,选用珍珠岩+草炭+蛭石(1:1:1)作为多花勾儿茶压条处理的基质,将珍珠岩、草炭、蛭石加水进行混合制得多花勾儿茶压条处理基质,S1. Substrate ratio: choose perlite + peat + vermiculite (1:1:1) as the substrate for the layering treatment of Catechus multiflora, and mix perlite, peat, and vermiculite with water to make Catecha multiflora layered substrate,

S2、生长调节剂种类确定,选用NAA萘乙酸作为多花勾儿茶压条处理扩繁的生长调节剂;S2, the type of growth regulator is determined, and NAA naphthaleneacetic acid is selected as the growth regulator for the multifloral layering treatment of Catechus multiflora;

S3、生长调节剂浓度配比,NAA萘乙酸浓度为400mg/L,再将配置好的生长调节剂涂刷至环剥伤口处及伤口周围,反复涂刷3次S3. The concentration ratio of the growth regulator, the concentration of NAA naphthalene acetic acid is 400mg/L, and then the prepared growth regulator is applied to the girdling wound and around the wound, and the brushing is repeated 3 times

S4、枝条选择,选择生长状况良好、树皮较光滑无损害、无病虫和寄生物附着的多花勾儿茶成年植株作为试验材料。挑选1-2年生的健康、长势旺盛、无机械损伤、茎粗0.5-0.8mm的枝条为压条枝S4, branch selection, select the adult plant of C. multiflora with good growth condition, smoother bark without damage, no diseases, insects and parasites attached as the test material. Select 1-2 year old healthy, vigorous growth, no mechanical damage, and 0.5-0.8mm thick stem branches as the pruning branches

S5、环剥处理:环剥宽度为8mm,S5, girdling treatment: girdling width is 8mm,

S6、压条处理操作:在压条枝上距基部50-100cm的光滑处进行造伤处理。修建掉压条枝上过多的分枝,用刻伤刀将枝条环状剥去皮层,环剥的深度以切断韧皮部,但不伤及木质部为原则,将形成层轻刮干净,根据不同的试验处理进行不同宽度的环剥,环剥后用70%酒精消毒,再将配置好的生长调节剂涂刷至伤口处及伤口周围,反复涂刷3次。将不同配比的基质土放入烘箱120℃高温灭菌3h,冷却后用蒸馏水浇透,填充进高压盒,包裹住切口部位,使伤口与基质土密切接触,将高压盒上下口扎好固定,避免基质流出。压条完成后,根据天气情况每3-4d采用注射器浇水一次。可以使多花勾儿茶繁殖生根成活率的影响试验;得出的压条生根率较高(90%以上),适合种苗扩繁。S6, layering treatment operation: perform injury treatment on the smooth part 50-100 cm away from the base of the layering branch. Prune off excessive branches on the plywood branches, peel off the cortex of the branches in a ring shape with a cutting knife, the depth of the girdling is based on the principle of cutting off the phloem, but not hurting the xylem, and scrape the cambium lightly. According to different experiments Girdling of different widths is carried out for treatment. After girdling, 70% alcohol is used to disinfect, and then the prepared growth regulator is applied to the wound and around the wound, and the brushing is repeated 3 times. Put different proportions of matrix soil into the oven for 120°C high-temperature sterilization for 3 hours, pour it thoroughly with distilled water after cooling, fill it into the high-pressure box, wrap the incision site, make the wound closely contact with the matrix soil, tie the upper and lower openings of the high-pressure box and fix it , to avoid matrix outflow. After the layering is completed, water it with a syringe every 3-4 days according to the weather conditions. The effect test on the rooting and survival rate of the multiflora catechuca can be carried out; the obtained layering rooting rate is higher (over 90%), which is suitable for seedling propagation.

本发明提供了一种多花勾儿茶压条处理扩繁方法,具备以下有益效果:The invention provides a method for processing and multiplication of Catechua multiflora by layering, which has the following beneficial effects:

1、该多花勾儿茶压条处理扩繁方法,通过选用珍珠岩、草炭、蛭石作为多花勾儿茶压条处理的基质,同时选用NAA萘乙酸作为多花勾儿茶压条处理扩繁的生长调节剂,浓度为400mg/L,同时选用环剥宽度8mm的环剥处理手段,可以使多花勾儿茶繁殖生根成活率的影响试验;得出的压条生根率较高(90%以上),其生根率高,适合种苗扩繁。1. The multiflora layering treatment expansion method, by selecting perlite, peat, and vermiculite as the substrate for multiflora layering, and simultaneously selecting NAA naphthaleneacetic acid as the multiflora layering processing expansion method Growth regulator, concentration is 400mg/L, selects the girdling treatment means of girdling width 8mm at the same time, can make the effect test of the survival rate of rooting of Catechus multiflora propagation; The layering rooting rate that draws is higher (more than 90%) , which has a high rooting rate and is suitable for seedling propagation.

附图说明Description of drawings

图1为本发明流程示意图。Fig. 1 is a schematic flow chart of the present invention.

具体实施方式Detailed ways

下面将结合本发明实施例中的附图,对本发明实施例中的技术方案进行清楚、完整地描述,显然,所描述的实施例仅仅是本发明一部分实施例,而不是全部的实施例。The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.

请参阅图1,本发明提供一种技术方案:一种多花勾儿茶压条处理扩繁方法,其方法包括以下步骤:Please refer to Fig. 1, the present invention provides a kind of technical scheme: a kind of multiflora catechu tea layering treatment multiplication method, its method comprises the following steps:

步骤1、基质配比:Step 1, matrix ratio:

选用珍珠岩、草炭、蛭石作为多花勾儿茶压条处理的基质,将珍珠岩、草炭、蛭石(1:1:1)加水进行混合制得多花勾儿茶压条处理基质,Perlite, peat, and vermiculite are selected as the substrate for the layering treatment of Glycine multiflora, and the perlite, peat, and vermiculite (1:1:1) are mixed with water to prepare the substrate for the layering treatment of Glycine multiflora.

步骤2、生长调节剂种类确定:Step 2, the type of growth regulator is determined:

选用NAA萘乙酸作为多花勾儿茶压条处理扩繁的生长调节剂,萘乙酸是一种有机化合物,分子式是C12H10O2,简称NAA,不溶于水,但微溶于热水,且在潮湿环境中易分解,具有植物生长调节剂的作用,但属于毒害品,在使用时应做好相关防护措施,通过选用NAA萘乙酸作为多花勾儿茶压条处理扩繁的生长调节剂,可以最大化促进多花勾儿茶压条处理扩繁的效率;NAA naphthalene acetic acid is selected as the growth regulator for multiflora layering treatment and multiplication. Naphthalene acetic acid is an organic compound with a molecular formula of C12H10O2, referred to as NAA. It is insoluble in water, but slightly soluble in hot water, and in a humid environment It is easy to decompose and has the function of plant growth regulator, but it is a poisonous product. Relevant protective measures should be taken when using it. By choosing NAA naphthalene acetic acid as the growth regulator for the multiflora layering treatment and multiplication, it can maximize the promotion The multiplication efficiency of multiflora Goucatecha layering treatment;

步骤3、生长调节剂浓度,NAA萘乙酸浓度为400mg/L,配置好后涂刷至伤口处及伤口周围,反复涂刷3次,Step 3. The concentration of the growth regulator, the concentration of NAA naphthalene acetic acid is 400mg/L. After the preparation is completed, apply it to the wound and around the wound, and repeatedly apply it 3 times.

步骤4、枝条选择:Step 4, branch selection:

选择生长状况良好、树皮较光滑无损害、无病虫和寄生物附着的多花勾儿茶成年植株作为试验材料。挑选1-2年生的健康、长势旺盛、无机械损伤、茎粗0.5-0.8mm的枝条为压条枝,可以极大的提高多花勾儿茶压条处理扩繁的效率,The adult plants of G. multiflora with good growth condition, smooth bark and no damage, no diseases, insects and parasites were selected as test materials. Select 1-2 year old healthy, vigorous growing, no mechanical damage, 0.5-0.8mm thick stem branches as layered branches, which can greatly improve the efficiency of multiflora Goucatecha layering treatment and multiplication,

步骤5、环剥处理:环剥宽度为8mm,根据实验我们得出在针对多花勾儿茶压条环剥处理的操作中,其环剥宽度保持在8mm,可以极大的提高多花勾儿茶压条繁殖的成活率,Step 5, girdling treatment: The girdling width is 8mm. According to the experiment, we have concluded that in the operation of girdling treatment for the multi-flowered catechu layer, the girdling width is kept at 8mm, which can greatly improve the multi-flowered hook. The survival rate of tea layering propagation,

步骤6、压条处理操作:在压条枝上距基部50-100cm的光滑处进行造伤处理。修建掉压条枝上过多的分枝,用刻伤刀将枝条环状剥去皮层,环剥的深度以切断韧皮部,但不伤及木质部为原则,将形成层轻刮干净,根据不同的试验处理进行不同宽度的环剥,环剥后用70%酒精消毒,再将配置好的生长调节剂涂刷至伤口处及伤口周围,反复涂刷3次。将不同配比的基质土放入烘箱120℃高温灭菌3h,冷却后用蒸馏水浇透,填充进高压盒,包裹住切口部位,使伤口与基质土密切接触,将高压盒上下口扎好固定,避免基质流出。压条完成后,根据天气情况每3-4d采用注射器浇水一次。得出的压条生根率较高(90-90%),适合种苗扩繁。Step 6, layering treatment operation: perform wounding treatment on the smooth place 50-100 cm away from the base on the layering branch. Prune off excessive branches on the plywood branches, peel off the cortex of the branches in a ring shape with a cutting knife, the depth of the girdling is based on the principle of cutting off the phloem, but not hurting the xylem, and scrape the cambium lightly. According to different experiments Girdling of different widths is carried out for treatment. After girdling, 70% alcohol is used to disinfect, and then the prepared growth regulator is applied to the wound and around the wound, and the brushing is repeated 3 times. Put different proportions of matrix soil into the oven for 120°C high-temperature sterilization for 3 hours, pour it thoroughly with distilled water after cooling, fill it into the high-pressure box, wrap the incision site, make the wound closely contact with the matrix soil, tie the upper and lower openings of the high-pressure box and fix it , to avoid matrix outflow. After the layering is completed, water it with a syringe every 3-4 days according to the weather conditions. The resulting layering has a higher rooting rate (90-90%) and is suitable for seedling propagation.

综上所述,该多花勾儿茶压条处理扩繁方法,使用时,首先对需要进行压条处理的多花勾儿茶枝条进行筛选,筛选后在压条枝上距基部50-100cm的光滑处进行环剥造伤处理,环剥后用70%酒精消毒,再将配置好的生长调节剂涂刷至伤口处及伤口周围,反复涂刷3次。将不同配比的基质土放入烘箱120℃高温灭菌3h,冷却后用蒸馏水浇透,填充进高压盒,包裹住切口部位,使伤口与基质土密切接触,将高压盒上下口扎好固定,避免基质流出。压条完成后,根据天气情况每3-4d采用注射器浇水一次,即可。To sum up, the multiflora catechu multiflora layering treatment multiplication method, when in use, first screens the multiflora catechu catechu branches that need to be layered, and after screening, it is placed on the smooth part of the layered branch 50-100cm away from the base. Perform girdling wound treatment, disinfect with 70% alcohol after girdling, and then apply the prepared growth regulator to the wound and around the wound, and repeatedly brush 3 times. Put different proportions of matrix soil into the oven for 120°C high-temperature sterilization for 3 hours, pour it thoroughly with distilled water after cooling, fill it into the high-pressure box, wrap the incision site, make the wound closely contact with the matrix soil, tie the upper and lower openings of the high-pressure box and fix it , to avoid matrix outflow. After the layering is completed, use a syringe to water once every 3-4 days according to the weather conditions.

最后应说明的几点是:首先,在本申请的描述中,需要说明的是,除非另有规定和限定,术语“安装”、“相连”、“连接”应做广义理解,可以是机械连接或电连接,也可以是两个元件内部的连通,可以是直接相连,“上”、“下”、“左”、“右”等仅用于表示相对位置关系,当被描述对象的绝对位置改变,则相对位置关系可能发生改变;The last few points should be explained: First, in the description of this application, it should be explained that, unless otherwise specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, which can be mechanical connection Or electrical connection, it can also be the internal communication of two components, it can be directly connected, "up", "down", "left", "right", etc. are only used to indicate the relative positional relationship, when the absolute position of the object being described Change, the relative positional relationship may change;

其次:本发明公开实施例附图中,只涉及到与本公开实施例涉及到的结构,其他结构可参考通常设计,在不冲突情况下,本发明同一实施例及不同实施例可以相互组合;Secondly: in the drawings of the disclosed embodiments of the present invention, only the structures related to the disclosed embodiments are involved, other structures can refer to the usual design, and the same embodiment and different embodiments of the present invention can be combined with each other if there is no conflict;

最后:以上所述仅为本发明的优选实施例而已,并不用于限制本发明,凡在本发明的精神和原则之内,所作的任何修改、等同替换、改进等,均应包含在本发明的保护范围之内。Finally: the above is only a preferred embodiment of the present invention, and is not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included in the present invention within the scope of protection.

Claims (2)

1.一种多花勾儿茶压条处理扩繁方法,其特征在于:其方法包括以下步骤:1. A method for multiflora catechu layering processing and multiplication, characterized in that: its method may further comprise the steps: S1、基质配比:S1. Substrate ratio: 选用珍珠岩、草炭、蛭石作为多花勾儿茶压条处理的基质,将珍珠岩+草炭+蛭石均匀混合后加水制得多花勾儿茶压条处理基质,Perlite, peat, and vermiculite were selected as the substrate for the layering treatment of Gou catechu multiflora, and the perlite+peat+vermiculite were evenly mixed and then water was added to prepare the layering treatment substrate for Gou catechu multiflora. S2、生长调节剂种类确定:S2. Determine the type of growth regulator: 选用NAA萘乙酸作为多花勾儿茶压条处理扩繁的生长调节剂,NAA naphthalene acetic acid was selected as the growth regulator for multiflora layering treatment and multiplication, S3、生长调节剂浓度配比,NAA萘乙酸浓度为400mg/L,S3, growth regulator concentration ratio, NAA naphthaleneacetic acid concentration is 400mg/L, S4、枝条选择:S4. Branch selection: 选择生长状况良好、树皮较光滑无损害、无病虫和寄生物附着的多花勾儿茶成年植株作为试验材料。挑选1-2年生的健康、长势旺盛、无机械损伤、茎粗0.5-0.8mm的枝条为压条枝。The adult plants of G. multiflora with good growth condition, smooth bark and no damage, no diseases, insects and parasites were selected as test materials. Choose 1-2 years old healthy, vigorous growth, no mechanical damage, and 0.5-0.8mm thick stems as the layered branches. S5、环剥处理:S5, girdling treatment: 环剥宽度为8mm,The girdling width is 8mm, S6、压条处理操作:S6, layering processing operation: 在压条枝上距基部50-100cm的光滑处进行造伤处理。修建掉压条枝上过多的分枝,用刻伤刀将枝条环状剥去皮层,环剥的深度以切断韧皮部,但不伤及木质部为原则,将形成层轻刮干净,根据不同的试验处理进行不同宽度的环剥,环剥后用70%酒精消毒,再将配置好的生长调节剂涂刷至伤口处及伤口周围,反复涂刷3次。将不同配比的基质土放入烘箱120℃高温灭菌3h,冷却后用蒸馏水浇透,填充进高压盒,包裹住切口部位,使伤口与基质土密切接触,将高压盒上下口扎好固定,避免基质流出。压条完成后,根据天气情况每3-4d采用注射器浇水一次。Injury treatment is carried out on the smooth part 50-100cm away from the base on the layered branch. Prune off excessive branches on the plywood branches, peel off the cortex of the branches in a ring shape with a cutting knife, the depth of the girdling is based on the principle of cutting off the phloem, but not hurting the xylem, and scrape the cambium lightly. According to different experiments Girdling of different widths is carried out for treatment. After girdling, 70% alcohol is used to disinfect, and then the prepared growth regulator is applied to the wound and around the wound, and the brushing is repeated 3 times. Put different proportions of matrix soil into the oven for 120°C high-temperature sterilization for 3 hours, pour it thoroughly with distilled water after cooling, fill it into the high-pressure box, wrap the incision site, make the wound closely contact with the matrix soil, tie the upper and lower openings of the high-pressure box and fix it , to avoid matrix outflow. After the layering is completed, water it with a syringe every 3-4 days according to the weather conditions. 2.根据权利要求1所述的一种多花勾儿茶压条处理扩繁方法,其特征在于:所述珠岩、草炭、蛭石的比例为1:1:1。2. A method for multiflora catechu multiflora layering treatment and multiplication according to claim 1, characterized in that: the ratio of perlite, peat and vermiculite is 1:1:1.
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