CN1160566C - Integrated chip capillary electrophoresis electrochemical detection device and manufacturing method thereof - Google Patents
Integrated chip capillary electrophoresis electrochemical detection device and manufacturing method thereof Download PDFInfo
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- CN1160566C CN1160566C CNB011284021A CN01128402A CN1160566C CN 1160566 C CN1160566 C CN 1160566C CN B011284021 A CNB011284021 A CN B011284021A CN 01128402 A CN01128402 A CN 01128402A CN 1160566 C CN1160566 C CN 1160566C
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- chip
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- glass
- capillary
- guiding tube
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- 238000005251 capillar electrophoresis Methods 0.000 title claims abstract description 9
- 238000000835 electrochemical detection Methods 0.000 title abstract description 6
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 239000011521 glass Substances 0.000 claims abstract description 41
- 238000001514 detection method Methods 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 10
- 238000009413 insulation Methods 0.000 claims abstract description 9
- 238000009434 installation Methods 0.000 claims abstract 2
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 claims description 12
- 229920005372 Plexiglas® Polymers 0.000 claims description 12
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 12
- 238000007789 sealing Methods 0.000 claims description 7
- 230000015572 biosynthetic process Effects 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 229910052697 platinum Inorganic materials 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 239000002699 waste material Substances 0.000 claims description 3
- 239000004593 Epoxy Substances 0.000 claims description 2
- 239000000853 adhesive Substances 0.000 claims description 2
- 230000001070 adhesive effect Effects 0.000 claims description 2
- 230000003292 diminished effect Effects 0.000 claims description 2
- 238000005755 formation reaction Methods 0.000 description 5
- 238000005234 chemical deposition Methods 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000007767 bonding agent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003822 epoxy resin Substances 0.000 description 1
- 229920000647 polyepoxide Polymers 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Abstract
The present invention provides a capillary electrophoretic electrochemical detection device with an integrated chip and a manufacture method thereof. The capillary electrophoretic electrochemical detection device is mainly composed of a micro disk electrode of glass insulation, a separating detection chip of capillary electrophoresis, an installation chip and a base of the micro disk electrode. The present invention is characterized in that the micro disk electrode is installed on the base by a movable electrode clamp and a guiding pipe aligning a channel outlet of the chip. Thus, working electrodes of the detection device can be replaced at any time according to the requirements, and any special-purpose equipment is not needed for manufacturing the detection device. The capillary electrophoretic electrochemical detection device is easily popularized and applied.
Description
Technical field
The present invention relates to a kind of integrated chip Capillary Electrophoresis chemical detection device, belong to the analytical chemistry field.
Background technology
Capillary electrophoretic electrochemical detector with integrated chip normally utilizes photoetch method that the split tunnel that gold electrode is fixed on chip is gone out the interruption-forming electrochemical investigating pond at present, perhaps on the wall around the split tunnel outlet of chip, spray one deck gold film as working electrode, perhaps working electrode is fixed in the exit of split tunnel end with chemical deposition, this class electrochemical detection device is method for making complexity, high to the making apparatus requirement not only, and working electrode can not change, thereby limited the application of pick-up unit.
Summary of the invention
The present invention is in order to simplify the method for making of this class electrochemical detection device, make the staff can utilize conventional equipment to produce the pick-up unit of replaceable electrode, the invention provides replaceable capillary electrophoretic electrochemical detector with integrated chip of a kind of electrode and preparation method thereof.
Capillary electrophoretic electrochemical detector with integrated chip provided by the invention comprises the microdisk electrode of glass insulation at least, the capillary electrophoresis separation detection chip, with the base that chip and microdisk electrode are installed, its special character is that chip is fixed on the glass slide, the tail end of glass slide forms a groove by installing two plexiglass blocks, the split tunnel that guiding tube is parallel to chip is fixed in the groove, and the tip of guiding tube is aimed at the split tunnel outlet and is kept certain distance to form detection cell, microdisk electrode is positioned at guiding tube, and plexiglass block is provided with platinum filament as the earth terminal that separates high pressure with chip chamber; One end of chip pad is for installing the platform of glass slide, and the other end is provided with the float electrode folder between spring and accurate screw rod; Glass capillary one end in the microdisk electrode of glass insulation is by drawing-down, its inside and outside diameter is diminished gradually, and wire electrode is positioned at kapillary, after wire electrode and thin end heating capillaceous are sealed, most advanced and sophisticated little dish, other end sealing capillaceous and the fixed electorde silk of forming of wire electrode.
The method for making of this capillary electrophoretic electrochemical detector with integrated chip comprises the steps: that (1) block at the former waste liquid slotted eye of capillary electrophoresis chip place, reserves the split tunnel outlet; (2) on the glass slide that the chip conglutinate is clean, be stained with two plexiglass blocks at the glass slide afterbody, stay a dead slot between two glass blocks along passage axis; (3) fix a platinum filament as the earth terminal that separates high pressure at plexiglass block and chip chamber; (4) the glass tube one end drawing-down with 2～3 millimeters of internal diameters makes guiding tube; (5) at microscopically the guiding tube parallel channels is put into dead slot, make its most advanced and sophisticated aligned with channel outlet and keep 80～120 microns; (6) insert the adhesive guiding tube at the dead slot rear portion; (7) the glass slide embedding is positioned on the organic glass platform of base one end, at other end mounting spring, float electrode folder, the accurate screw rod of base; (8) an end drawing-down of the glass capillary that inside and outside footpath is suitable is to requiring size; (9) one one termination is had the wire electrode of lead insert in the kapillary, wire electrode exposes capillary tip, and heats and seal, and makes the little dish of the most advanced and sophisticated formation of wire electrode; (10) other end capillaceous makes the microdisk electrode of glass insulation with epoxy sealing and with lead fixed; (11) microdisk electrode is inserted guiding tube, its rear end is adjusted, is fixed with the float electrode folder.
As shown from the above technical solution, capillary electrophoretic electrochemical detector with integrated chip of the present invention is that the microdisk electrode that will insulate is installed on the base by electrode holder, guiding tube, so just, can require to change different working electrodes according to detecting, and as can be known from its method for making, this method need not make uses up candle method at quarter, chemical deposition or the required specialized equipment of spraying process, and making step is also easy, so this pick-up unit is very easily promoted application.
Description of drawings
Accompanying drawing 1 is an one-piece construction shaft side figure of the present invention.
Accompanying drawing 2 is the I portion partial enlarged drawing of accompanying drawing 1.
Accompanying drawing 3 is the structural representation of microdisk electrode.
Embodiment
As Fig. 1, shown in Figure 2, capillary electrophoretic electrochemical detector with integrated chip provided by the invention is mainly by the microdisk electrode 17 of glass insulation, formations such as capillary electrophoresis separation detection chip 7 and base 12, chip 7 is fixed on the glass slide 2, the tail end of glass slide 2 forms a groove by installing two plexiglass blocks 3, the split tunnel 1 that guiding tube 5 is parallel to chip 7 is fixed in the groove, and the tip of guiding tube 5 is aimed at the outlet of split tunnel 1 and is kept certain distance to form detection cell, plexiglass block 3 is provided with platinum filament 6 as the earth terminal that separates high pressure with 7 of chips, glass slide 2 is installed on the platform 8 that is positioned at base 12 1 ends, the other end of base 12 is provided with float electrode folder 9 at spring 11 and 10 of accurate screw rods: as shown in Figure 3, glass capillary 14 1 ends in the microdisk electrode 17 are by drawing-down, make in it, overall diameter diminishes gradually, wire electrode 13 is positioned at kapillary 14, after wire electrode 13 seals with the thin end heating of kapillary 14, the little dish of the most advanced and sophisticated formation of wire electrode, usually the rear end of wire electrode 13 is connected with slightly thick lead 15, the other end sealing and the fixing cord 15 of kapillary 14, microdisk electrode 17 is positioned at guiding tube, by guiding tube 5, float electrode folder 9 is installed on the base 12.
When making this pick-up unit, can be by poly-carrying out of following step; (1) blocks at capillary electrophoresis chip 7 former waste liquid slotted eye places, stay the also outlet of split tunnel 1; (2) on the glass slide 2 that chip 7 conglutinates are clean, be stained with 3 of two plexiglass blocks, 3, two glass blocks along the axis of passage 1 at glass slide 2 afterbodys and stay a dead slot; (3) fix a platinum filament 6 as the earth terminal that separates high pressure 7 of plexiglass block 3 and chips; (4) the glass tube one end drawing-down with 2～3 millimeters of internal diameters makes guiding tube 5; (5) microscopically is put into dead slot with guiding tube 5 parallel channels 1, makes the outlet of its most advanced and sophisticated aligned with channel 1 and keeps 80～120 microns; (6) insert bonding agent 4 fixed guide pipes 5 at the dead slot rear portion; (7) glass slide 2 embeddings are positioned on the organic glass platform 8 of base 12 1 ends, at other end mounting spring 11, float electrode folder 9, the accurate screw rod 10 of base 12; (8) an end drawing-down of the glass capillary 14 that inside and outside footpath is suitable is to requiring size; There is the wire electrode 13 of lead 15 to insert in the kapillary 14 one one termination, wire electrode 13 exposes kapillary 14 tips, and heating is sealed, make the little dish of wire electrode 13 most advanced and sophisticated formation, usually in order to guarantee the formation of little dish, can vertically clip one section wire electrode of sealing 13 with blade after once heating is sealed, Fast Heating is sealed the tip that can guarantee electrode wires 13 and is formed little dish once more; (10) other end of kapillary 14 and epoxy resin 16 sealings are fixing with lead 15, make the microdisk electrode 17 of glass insulation; (11) microdisk electrode 17 is inserted guiding tube 5, its rear end is adjusted, is fixed with float electrode folder 9.
Claims (3)
1, a kind of capillary electrophoretic electrochemical detector with integrated chip, at least the microdisk electrode that comprises glass insulation, the capillary electrophoresis separation detection chip, and the base of installation chip and microdisk electrode, it is characterized in that: an end of base is for installing the platform of glass slide, chip is fixed on the glass slide, the tail end of glass slide forms a groove by installing two plexiglass blocks, the split tunnel that guiding tube is parallel to chip is fixed in the groove, and the tip of guiding tube is aimed at the split tunnel outlet and is kept certain distance to form detection cell, microdisk electrode is positioned at guiding tube, plexiglass block is provided with platinum filament as the earth terminal that separates high pressure with chip chamber, and the other end of base is provided with the float electrode folder between spring and accurate screw rod.
2, capillary electrophoretic electrochemical detector with integrated chip according to claim 1, it is characterized in that: glass capillary one end in the microdisk electrode of glass insulation is by drawing-down, its inside and outside diameter is diminished gradually, wire electrode is positioned at kapillary, after wire electrode and thin end heating capillaceous are sealed, most advanced and sophisticated little dish, other end sealing capillaceous and the fixed electorde silk of forming of wire electrode.
3, according to the method for making of the described capillary electrophoretic electrochemical detector with integrated chip of claim 1, comprise the steps: that (1) block at the former waste liquid slotted eye of capillary electrophoresis chip place, reserve the split tunnel outlet; (2) on the glass slide that the chip conglutinate is clean, be stained with two plexiglass blocks at the slide afterbody, stayed poor between two glass blocks along passage axis; (3) fix a platinum filament as the earth terminal that separates high pressure at plexiglass block and chip chamber; (4) the glass tube one end drawing-down with 2～3 millimeters of internal diameters makes guiding tube; (5) at microscopically the guiding tube parallel channels is put into dead slot, make its most advanced and sophisticated aligned with channel outlet and keep 80～120 microns; (6) insert the adhesive guiding tube at the dead slot rear portion; (7) glass slide is embedded on the organic glass platform of base, at other end mounting spring, float electrode folder, the accurate screw rod of base; (8) an end drawing-down of the glass capillary that inside and outside footpath is suitable is to requiring size; (9) one one termination is had the wire electrode of lead insert in the kapillary, wire electrode exposes capillary tip, and heats and seal, and makes the little dish of the most advanced and sophisticated formation of wire electrode; (10) other end capillaceous makes the microdisk electrode of glass insulation with epoxy sealing and with lead fixed; (11) microelectrode is inserted guiding tube, first float electrode folder in its rear end is adjusted, is fixed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011284021A CN1160566C (en) | 2001-09-03 | 2001-09-03 | Integrated chip capillary electrophoresis electrochemical detection device and manufacturing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011284021A CN1160566C (en) | 2001-09-03 | 2001-09-03 | Integrated chip capillary electrophoresis electrochemical detection device and manufacturing method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1336543A CN1336543A (en) | 2002-02-20 |
| CN1160566C true CN1160566C (en) | 2004-08-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB011284021A Expired - Fee Related CN1160566C (en) | 2001-09-03 | 2001-09-03 | Integrated chip capillary electrophoresis electrochemical detection device and manufacturing method thereof |
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| Country | Link |
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| CN (1) | CN1160566C (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100429511C (en) * | 2004-04-09 | 2008-10-29 | 南京大学 | Electrochemical detection method and device of integrated in chip capillary electrophoresis |
| JP5320416B2 (en) * | 2011-01-26 | 2013-10-23 | 株式会社日立ハイテクノロジーズ | Electrophoresis device, capillary array, and capillary unit |
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2001
- 2001-09-03 CN CNB011284021A patent/CN1160566C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1336543A (en) | 2002-02-20 |
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