CN115844762A

CN115844762A - Gargle for improving microenvironment of oral cavity and improving immunity of oral cavity and preparation method thereof

Info

Publication number: CN115844762A
Application number: CN202310006791.8A
Authority: CN
Inventors: 孙先昌; 金忠; 郝昱凯
Original assignee: Beijing Lanhai Medical Technology Co ltd
Current assignee: Beijing Lanhai Medical Technology Co ltd
Priority date: 2022-12-27
Filing date: 2023-01-04
Publication date: 2023-03-28

Abstract

The embodiment of the invention discloses gargle for improving the microenvironment of an oral cavity and improving the immunity of the oral cavity and a preparation method thereof. A gargle for improving the microenvironment of the mouth and enhancing the immunity of the mouth, which comprises carbomer, an antioxidant, a probiotic growth factor, vitamin D and purified water; the antioxidant is selected from one or more of tea polyphenol, vitamin C ethyl ether and vitamin E; the probiotic growth factor is selected from one or more of trehalose, isomaltooligosaccharide and fructo-oligosaccharide; the viscosity value of the gargle is 100-400mpa.s. The gargle provided by the invention can effectively regulate the immunocompetence of intestinal flora, improve the immunocompetence of the oral cavity, reduce the occurrence of oral diseases, and is worthy of popularization and application.

Description

Gargle for improving mouth microenvironment and mouth immunity and preparation method thereof

Technical Field

The embodiment of the invention relates to the technical field of oral care and health care, in particular to gargle for improving the microenvironment of an oral cavity and improving the immunity of the oral cavity and a preparation method thereof.

Background

With the improvement of living standard of people, the demand of consumers on oral cleaning products is more and more, and the demand is higher and higher. The functions of the existing oral cleaning products mainly focus on improving breath, inhibiting bacteria and the like immediately, and the oral micro-ecological disorder caused by the oral cleaning products is not considered. Meanwhile, the dietary habits of Chinese people are also changing, particularly young people excessively pursue taste and stimulation, so that oral problems are more and more serious, including oral inflammations such as oral ulcer, gingivitis and periodontitis, and the like are more and more common.

The mouthwash can clean the oral cavity at any time and any place and is popular, but various mouthwashes in the current market have the main functions of cleaning the oral cavity, disinfecting and sterilizing and conditioning the oral cavity, the retention time of the mouthwashes in the oral cavity is short, and the effect of the functional components in a short time is limited.

Billions of microorganisms live in the mouth, with bacteria ranging up to over 700 species. The oral flora is the second largest flora in humans. Most of these bacteria are beneficial, can fight disease, aid digestion, and regulate body functions, and have important effects on oral health, such as: can improve the metabolism, self-purification, self-repair and balance ability of oral cells and maintain the health of the oral cavity. Meanwhile, some harmful bacteria exist in the oral cavity, and the imbalance of the oral flora can cause diseases of the oral cavity. The oral cavity is used as a connection part between the interior of a human body and the external environment, and the microbial community in the oral cavity is not only related to oral diseases, but also is obviously related to whole body and mind or chronic diseases. Under physiological state, dynamic balance exists among oral microorganisms and between the microorganisms and hosts, and the oral microorganisms participate in organism metabolism, immunity and nutrition and maintain oral health together. However, under the influence of various external factors such as physicochemical factors, bacterial factors, host factors and the like, such as improper or excessive oral cavity cleaning and the like, the micro-ecosystem balance of the oral cavity is broken, beneficial bacteria are reduced and cannot be rapidly proliferated, harmful bacteria are increased, the harmful bacteria and toxin generated by the harmful bacteria can damage the oral health, so that related oral diseases such as decayed teeth, periodontal disease, gingivitis, recurrent oral ulcer and the like can be caused, and the imbalance of flora of various oral diseases can be aggravated, so that the regulation of the oral flora balance is the key for preventing and treating various oral diseases.

The prior oral cleaning products mostly use probiotics, for example, chinese patent CN202111137085.4 discloses a lactobacillus plantarum N13 and application thereof in preventing or treating dental caries and periodontitis, the provided lactobacillus plantarum N13 has strong capability of inhibiting growth of streptococcus mutans, has high tolerance concentration to lysozyme, is easy to colonize in oral cavity, and the lactobacillus plantarum N13 also has strong self-gathering capability and copolymerization capability with streptococcus mutans, and can be applied to functional foods, medicines or nursing products for preventing or treating dental caries and periodontitis. Chinese patent CN202111453744.5 discloses a composition compounded by probiotics and tea polyphenol, a preparation and application thereof, and the application proves that the composition realizes a coordinated antibacterial effect through co-coagulation and sterilization effects by using methods such as a scanning electron microscope, an atomic force microscope, fourier infrared spectroscopy, surface charge analysis and the like, and is used for preventing and treating oral diseases and applied to oral cleaning and nursing products such as toothpaste and the like. However, the oral care products containing probiotics have high production process difficulty and cost, are difficult to endure adverse environments such as high temperature and high salt, and have activity which is difficult to store for a long time, thereby causing difficulties in storage, transportation, production and other links.

The market mainstream products pursue short-term effects, can not treat both symptoms and root causes, can treat both symptoms and root causes for a long time, can cause the continuous deterioration of the microenvironment of the oral cavity, and have increasingly serious problems of halitosis and even oral inflammation. Therefore, how to improve the long-acting effect of the oral cavity gargle product and solve the problems related to the oral cavity in a mode of treating both symptoms and root causes is one of the key technical problems which are always addressed by the technical personnel in the field.

Disclosure of Invention

Therefore, the embodiment of the invention provides the gargle for improving the microenvironment of the oral cavity and improving the oral immunity and the preparation method thereof, and the gargle can adjust the micro-ecological balance of the oral cavity, improve the oral immunity and fundamentally prevent and treat various oral diseases.

In order to achieve the above object, the embodiments of the present invention provide the following technical solutions:

according to a first aspect of embodiments of the present invention, there is provided an oral mouthrinse for improving the microenvironment of the mouth and for enhancing the immunity of the mouth, the mouthrinse comprising carbomer, an antioxidant, a probiotic growth factor, vitamin D and purified water; the antioxidant is selected from one or more of tea polyphenol, vitamin C ethyl ether and vitamin E; the probiotic growth factor is selected from one or more of trehalose, isomaltooligosaccharide and fructo-oligosaccharide; the gargle has a viscosity value of 100-400mpa.s.

The existing gargle liquid products have low viscosity, short acting time in the oral cavity and unsatisfactory effect. Carbomer solutions are non-newtonian liquids that shear thin when gargling in the mouth and become more viscous and adhere to the oral mucosa when gargling ceases. Carbomer, as a carboxyl high molecular polymer, is a very ideal bioadhesive and has very strong bioadhesion, and is widely applied to common nasal bioadhesives, bioadhesives for use in rectum bioadhesives, buccal bioadhesives or oral bioadhesives. Therefore, the carbomer aqueous solution is used as a liquid suspension system and a biological adhesion system, and researches show that when the viscosity range of the gargle is 100-400mpas, the gargle has good mouth-rinsing taste and good adhesion effect, and the better mouth-rinsing effect is realized.

The action of the above bioactive ingredients will now be described as follows:

tea polyphenol is a natural antioxidant and has a clearing effect on various active oxygen free radicals, so that a free radical reaction chain in vivo is blocked, and biomacromolecule damage caused by surplus free radicals is eliminated, and the mechanism of the tea polyphenol mainly comprises the following components: inhibiting free radical generation, directly scavenging active oxygen free radicals, inhibiting lipid peroxidation, chelating metal ions, activating intracellular antioxidant defense system, etc. The results of in vitro experiments, animal experiments and human intervention researches show that the tea polyphenol has high-efficiency antioxidant function, thereby being beneficial to cancer prevention, cancer resistance, mutation resistance, aging resistance, cardiovascular disease prevention and treatment and the like. Tea polyphenol contains more free hydroxyl groups, and can be combined with protein and amino acid to precipitate protein to cause bacterial death, which is the basis of the bactericidal and antiviral effects of tea polyphenol. The tea polyphenols can change physiological structure of pathogenic bacteria, interfere metabolism of pathogenic bacteria, inhibit activity of bacterial toxin, inhibit and kill bacteria, and reduce invasion of bacteria and toxin to oral mucosa. In many antibacterial experiments, the antibacterial agent is found to be used for treating oral ordinary proteus, antibiotic-resistant staphylococcus, streptococcus mutans and main pathogenic bacteria of oral cavity and throat: pneumococcal, staphylococcus epidermidis, candida b sensitive and periodontal disease related bacteria: the fusobacterium necrophorum, the furin gingivalis and the like have inhibiting and killing effects in different degrees. Research shows that the tea polyphenol has inhibition rate of 98.3% on staphylococcus and colibacillus, and can inhibit glucose polymerization activity of acid-producing bacteria and activity of human salivary amylase, prevent adhesion of thallus to oral mucosa and inhibit oral diseases. The tea polyphenol is beneficial to the growth and reproduction of oral probiotic groups by inhibiting oral pathogenic bacteria, thereby improving the oral micro-ecological environment.

Vitamin C can promote lymphoblast generation, stimulate lymphocyte proliferation reaction, improve recognition and phagocytosis of foreign or malignant cells, and improve phagocyte activity. The vitamin C taken by healthy people can enhance the chemotaxis of circulating blood neutrophils. Vitamin C can also improve the neutrophil migration and bactericidal function of the person with the abnormal immune function; vitamin C can promote the proliferation of T cells and NK cells and affect their cellular function. The biggest problem in the application of the vitamin C is that the vitamin C can be stabilized only when the pH value is lower than 3.5, so that the amphiphilic vitamin C ethyl ether is selected in the invention, and the vitamin C can stably exist and play the same role as the vitamin C even when the pH value reaches 6.5.

Vitamin E has two routes to oral care or disease prevention: (1) by using the oral care product (the paste or liquid containing vitamin E), the vitamin E is deposited and adsorbed on the surface of the oral mucosa, absorbed by the oral mucosa and enters blood to care and protect the whole oral cavity; (2) directly acts on pathogenic bacteria, viruses and lesion tissues in the oral cavity; the vitamin E has a lipophilic and hydrophobic structure, can enter a biological membrane containing unsaturated fatty acid, and can directly capture free radicals in a lipid phase medium, thereby interrupting the propagation and diffusion of unsaturated fatty acid peroxidation in a membrane lipid bilayer structure, maintaining the stability of the composition of the unsaturated fatty acid on the membrane, effectively terminating the chain reaction of free radicals caused by lipid peroxidation, being one of important mechanisms for preventing cells from being damaged, and being one of the most main biological free radical scavengers and antioxidants in vivo.

Fructo-oligosaccharide and isomaltooligosaccharide are considered as the most typical bifidus factors, and are often used as probiotic preparations, so that the digestion and absorption of a human body are facilitated, and meanwhile, the growth and proliferation of intestinal probiotics are promoted, the intestinal microecology is regulated, and the immunity of the human body is improved. The fructo-oligosaccharide is a naturally-occurring substance, has good activity of promoting the proliferation of bifidobacteria, can induce the mass propagation of beneficial bacteria represented by bifidobacteria in intestinal tracts, improve the microbial environment of the intestinal tracts, improve the immunity of human bodies, combine toxic substances in the intestines and promote the discharge of the harmful substances out of the bodies. Isomaltooligosaccharides are tetrasaccharides and pentasaccharides, which are formed from isomaltose combined with one molecule of glucose. Can be used in low-energy food, can meet the requirements of people who like sweetmeats and worry about obesity to the maximum extent, and can be used for diabetes patients, obesity patients and hypoglycemia patients.

Trehalose is a typical stress metabolite, can form a unique protective film on the surface of cells under severe environmental conditions such as high temperature, high cold, high osmotic pressure, dry dehydration and the like, and effectively protects a biological molecular structure from being damaged, so that the life process and biological characteristics of a living body are maintained. Trehalose is used as a proliferation factor of in vivo beneficial intestinal bacteria, namely bifidobacterium, can improve the intestinal micro-ecological environment, strengthen the digestive absorption function of gastrointestinal tracts, effectively eliminate vivotoxin and enhance the immunity and disease resistance of organisms, and researches also prove that the trehalose has stronger radiation resistance.

The human digestive system and respiratory system are two main systems for exchanging external substances with human, wherein intestinal flora in the digestive system has great influence on the immune system of the organism, and the research proves that 70-80% of immune cells are distributed in intestinal lymphoid tissues, and the oral cavity is used as the front of the digestive system and is inseparable with the digestive system and the whole body immune relationship. Thus, modulating the immune competence of the gut flora also affects the immune competence of the oral cavity.

Vitamin D is a fat-soluble vitamin, and has the main function of promoting the absorption of calcium and phosphorus by small intestine mucosal cells, and the lack of vitamin D can cause tooth decay and tooth loosening. Research results show that vitamin D also plays a great role in regulating the human immune system. Vitamin D is involved in regulation of more than 2000 genes of the human genome, including anti-infective immunity, enhancing immunity to pathogens. It was found that almost all cells of the immune system carry vitamin D receptors, that is: vitamin D can support the innate and acquired immune system of the human body. Vitamin D also allows normal human bone marrow cells to differentiate into macrophages and monocytes, which may be a link in regulating immune function. Therefore, the vitamin D has an immunoregulation effect and is a good selective immunoregulator. When the immune function of the body is inhibited, vitamin D can enhance the functions of monocytes and macrophages, thereby enhancing the immune function.

The vitamin D, the antioxidant and the probiotic growth factor play a role together, so that the effects of cleaning the oral cavity and improving breath are achieved, the micro-environment of the oral cavity is effectively improved, and the systemic immunity mainly comprising the oral cavity is improved.

Further, the carbomer is carbomer 971P or carbomer 974P; based on the mass of the gargle, the mass percentage of the carbomer is 0.1-0.3%. In the long-term research process, the applicant finds that almost all high molecular materials, whether natural high molecules or artificially synthesized high molecules, can cause the molecular weight to be reduced sharply by radiation sterilization, and in the case of sodium hyaluronate, 25KGY radiation sterilization can cause the viscosity of the product to be lost almost completely, so that the sodium hyaluronate product is selected to be sterilized by autoclaving. The applicant finds that radiation sterilization has different influences on high polymers including carbomer in the long-term cooperative research process with carbomer production enterprises, and the influence is mainly related to the type of carbomer and the concentration of a solution. On the basis of a large number of experiments, the carbomer type and concentration are screened, so that the carbomer is slightly influenced when radiation sterilization is carried out, and the gargle is more favorably adjusted to 100-400mpas in viscosity.

Further, the antioxidant is prepared from the following components in a mass ratio of 0.05-0.3:0.05-0.3:0.1-2 of tea polyphenol, vitamin C ethyl ether and vitamin E. The invention further discovers that when the tea polyphenol, the vitamin C ethyl ether and the vitamin E are used simultaneously according to the dosage, the synergistic complementary effect can be achieved in different lipophilic/hydrophilic microcosmic environments inside/outside the mucous membrane of the oral cavity, free radicals are eliminated, oral cavity probiotic groups are protected, the oral cavity immunity is improved, and the occurrence of oral cavity diseases is reduced.

Further, the probiotic growth factor is prepared from the following components in a mass ratio of 0.2-2:0.5-3:0.5-3 parts of trehalose, isomaltooligosaccharide and fructo-oligosaccharide. The invention further discovers that fructo-oligosaccharide, isomaltooligosaccharide and trehalose are compounded and applied to oral cleaning products according to the dosage, so that the growth and proliferation of intestinal probiotics can be promoted, the intestinal microecology can be adjusted, the immunity of an organism can be improved, the immunity of the oral cavity can be correspondingly improved, and the barrier capability of a connecting channel between the oral cavity and the outside can be enhanced.

Further, the gargle also comprises additives, wherein the additives are selected from one or more of sweeteners, humectants, flavoring agents, emulsifying agents and pH regulators.

Further, the sweetener is xylitol; the humectant is glycerin; the flavoring agent is eugenol or menthol; the emulsifier is polyoxyethylene hydrogenated castor oil; the pH regulator is potassium hydroxide.

In some specific embodiments, the gargle is prepared from the following raw materials in percentage by weight: 0.1-0.3% of carbomer, 0.05-0.3% of tea polyphenol, 0.05-0.3% of vitamin C ethyl ether, 0.1-2% of vitamin E, 0.2-2% of trehalose, 0.5-3% of isomaltooligosaccharide, 0.5-3% of fructo-oligosaccharide, 0.01-0.1% of vitamin D, 0.5-5% of xylitol, 1-10% of glycerol, 0.1-1.0% of eugenol, 0.05-0.5% of menthol, 0.5-3.0% of polyoxyethylene hydrogenated castor oil, a proper amount of potassium hydroxide, the pH value is adjusted to be 6.0-7.0, and the balance of purified water. The invention further discovers that the gargle provided by the invention has excellent mouthfeel by optimizing the composition and content of the bioactive components and the auxiliary materials of the gargle.

According to a second aspect of embodiments of the present invention, there is provided a method of preparing an oral rinse as described above, comprising the steps of:

(1) Preparing raw materials according to a formula proportion;

(2) Uniformly stirring carbomer and glycerol, adding 50-80% purified water, and stirring until carbomer is completely dissolved to obtain material A;

(3) Adding vitamin C ethyl ether, vitamin E, vitamin D, eugenol and menthol into polyoxyethylene hydrogenated castor oil, stirring uniformly, adding a proper amount of purified water, and stirring until the materials are completely dissolved to obtain a material B;

(4) Mixing the material A and the material B, then adding tea polyphenol, trehalose, isomaltooligosaccharide, fructo-oligosaccharide and xylitol, and stirring until the materials are completely dissolved to obtain a material C;

(5) Adding a proper amount of potassium hydroxide into the material C, adjusting the pH value to 6.0-7.0, adding the balance of purified water, and uniformly stirring to obtain a material D, wherein the viscosity value of the material D is 300-600mpa.s;

(6) And (3) performing radiation sterilization on the material D at 25KGY to obtain the gargle, wherein the viscosity value of the gargle is 100-400mpa.s.

The invention adopts a radiation sterilization mode to carry out aseptic treatment, avoids the use of disinfectants and bacteriostats, reduces the adverse effect of the use of products on normal flora of the oral cavity and provides a basic guarantee for the maintenance of the micro-ecological balance of the oral cavity.

Further, in the step (2), after the purified water is added, the rotating speed is adjusted to 300-1500rpm, stirring is continued for 1-5 hours, when a large amount of bubbles are visible, the rotating speed is adjusted to 100-300rpm, stirring is carried out until the bubbles are obviously reduced, and when no lumps exist in the material, the stirring speed is increased until the carbomer is completely dissolved.

Further, in the step (3), the volume of the purified water is 2-3 times of the volume of the polyoxyethylene hydrogenated castor oil.

The embodiment of the invention has the following advantages:

(1) By utilizing the characteristics that the carbomer solution is a non-Newtonian liquid, a bioadhesive and the like, the radiation sterilization influence is fully considered, the type and concentration of the special carbomer are selected, the viscosity of the carbomer solution is adjusted, a better gargle effect is achieved, and the gargle is ensured to play a continuous role in the oral cavity.

(2) The tea polyphenol, the VC ethyl ether and the vitamin E are used simultaneously, so that a synergistic complementary effect can be achieved in different micro environments of inner/outer and lipophilicity/hydrophily of oral mucosa, free radicals are eliminated, probiotic groups are protected, oral immunity is improved, and oral diseases are reduced.

(3) The compound application of the fructo-oligosaccharide, the isomaltooligosaccharide and the trehalose improves the immunity of the oral cavity and reduces the occurrence of oral diseases by regulating the immunity of intestinal flora.

(4) Vitamin D has immunoregulation effect, and is a good selective immunomodulator.

(5) The product is provided in a sterile manner, is disposable, does not contain a disinfectant, does not need to add a preservative, can be used by common people for a long time, and is suitable for pregnant and lying-in women, children and people with low immunity.

Detailed Description

The present invention is described in terms of specific embodiments, and other advantages and benefits of the present invention will become apparent to those skilled in the art from the following disclosure. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

This example provides optimization of the type of carbomer and the concentration of the solution.

Sample 1: carbomer type 974P; sample 2: the baum number is 971P; sample 3: carbomer type 974P. Different types of carbomers were sterilized with electron beam 25KGY and the results of the viscosity change before and after sterilization are shown in table 1.

TABLE 1

Sample number	Viscosity of the oil _{Before sterilization} (mpa.s)	Viscosity of the oil _{After sterilization} (mpa.s)
			1	515	382
1	504	375
			1	501	379
2	354	278
			2	336	273
2	347	270
			3	242	184
3	235	179
			3	233	175

0.2%974P, electron beam 25KGY sterilization, three replicates dispersed in artificial saliva (stain added to solution), results are shown in Table 2.

TABLE 2

Before sterilization (min)	After sterilization (min)	A control with a viscosity of less than 50mpa.s (min)
			21	16	Less than 2
22	18	Less than 2
			24	17	Less than 2

As can be seen from the results of the in vitro simulation shown in table 2, the viscosity plays an important role in the oral-like system, and the retention time can be significantly prolonged when the viscosity of the product is 100-400mpa.s.

Example 2

This example provides a gargle having a total formulation of 1000g. The raw materials are as follows:

carbomer 971P 2g;

10g of tea polyphenol, 10g of vitamin C ethyl ether and 10g of vitamin E;

10g of trehalose, 10g of isomaltooligosaccharide and 10g of fructo-oligosaccharide;

vitamin D31 g;

10g of xylitol, 10g of glycerol, 5g of eugenol, 1g of menthol, 4030 g of RH and a proper amount of potassium hydroxide; the balance being purified water.

The preparation method of the gargle comprises the following steps:

(1) Uniformly stirring carbomer and glycerol, adding 750g of purified water, adjusting the rotation speed to 800rpm, stirring for 3 hours continuously until a large amount of bubbles are visible, then adjusting the rotation speed to 200rpm until the bubbles are obviously reduced, and observing that no lump in a beaker accelerates the stirring speed until the carbomer is completely dissolved;

(2) Adding vitamin C ethyl ether, vitamin E, vitamin D3, eugenol and menthol into RH40, stirring and dispersing uniformly, adding 70g of purified water, stirring until the solid components are completely dissolved, and then adding into the carbomer solution in the step (1);

(3) Adding tea polyphenol, trehalose, isomaltooligosaccharide, fructo-oligosaccharide and xylitol, and stirring at 200rpm for 1 hour until completely dissolving;

(4) Adjusting pH to 6.5 with 10% potassium hydroxide aqueous solution, adding purified water to make up to 1000g, stirring well, and testing to obtain viscosity of 375mpa.s.

(5) And (5) carrying out radiation sterilization on 25KGY, and measuring the viscosity after sterilization, wherein the viscosity value is 315mpa.s.

Example 3

This example provides a gargle having a total formulation of 1000g. The raw materials comprise the following components in percentage by weight:

the carbomer accounts for 974P 10g;

2g of tea polyphenol, 2g of vitamin C ethyl ether and 5g of vitamin E;

5g of trehalose, 5g of isomaltooligosaccharide and 5g of fructo-oligosaccharide;

32 g of vitamin D;

15g of xylitol, 1g of eugenol, 2g of menthol, RH4040 g and 20g of glycerol; proper amount of potassium hydroxide; the balance being purified water.

The preparation method of the gargle is the same as that of example 2, wherein the viscosity value of the solution obtained in the step (4) is 515mpa.s, and the viscosity value of the solution obtained in the step (5) is 372mpa.s.

Test example 1

Halitosis test

1. Basic data

1. Intelligent breath detector

An intelligent breath tester (trade name: shownovo). The bad breath is mostly caused by the methyl mercaptan (CH) generated in oral hygiene, oral diseases and gastrointestinal diseases ₃ SH) and hydrogen sulfide (H) ₂ S). Shownovo intelligence halitosis detector can the accurate main volatility sulphide that measures halitosis gas, and the effectual halitosis of science is administered the monitoring. The Shownovo intelligent breath detector measures the breath smell in the oral cavity, instantly judges the result to be 1-4 grades, and simultaneously outputs a specific gas concentration value and a suggested social distance.

Level 1

The gas concentration is: 0.1ppm-0.5ppm, suggested social distance: 20-65cm. The degree of breath is slight: belongs to the acceptable category and does not need worry.

Level 2

The gas concentration is: 0.6ppm-1.0ppm, suggested social distance: 65-90cm, moderate degree of breath: the electric toothbrush, the toothpaste for inhibiting oral odor, the tooth flusher and the like are selected to improve the oral environmental sanitation, and the tooth washing is recommended once a year.

Level 3

The gas concentration is: 1.0ppm-2.5ppm, suggested social distance: the mouth smell degree of 90-120cm is serious: it is recommended to pay attention to the diseases around the oral cavity, such as periodontitis, decayed teeth, nasopharyngitis, etc., and to visit the clinic in the oral cavity.

Level 4

The gas concentration is: >2.5ppm, suggested social distance: 120cm

Bad breath degree: people need to pay attention to the health, and halitosis can be related to pathological diseases, such as digestive system diseases, respiratory system diseases, diabetes, liver diseases and the like, and is recommended to a regular hospital for systematic investigation and treatment.

2. Related data

Relevant studies have shown a strong correlation between the level of oral malodor and the concentration of VSCs hydrogen sulfide and methyl mercaptan in the breath of the mouth. Considering the much larger odor index of methyl mercaptan, it was concluded that methyl mercaptan is the major cause of oral malodor.

Physiological halitosis is generally a condition in which the concentration of hydrogen sulfide is higher than that of methyl mercaptan;

pathological halitosis in the case of periodontal or gingival disease, the concentration of methyl mercaptan can be up to three times the concentration of hydrogen sulfide.

Many bacteria in the oral cavity are able to utilize methionine, cysteine and serum to produce CH ₃ SH and H ₂ S, 4 bacteria (Porphyromonas gingivalis, treponema denticola, prevotella intermedia, fostana) associated with periodontal disease showed more active sulfur-producing ability. Porphyromonas gingivalis can not only produce CH using L-methionine ₃ SH, at the same time, also possibleProduction of H using cysteine ₂ S, and treponema denticola produced large amounts of CH even at very low L-methionine concentrations ₃ And (5) SH. The results of a laboratory study at the oral academy of seoul university show that periodontal pathogens also produce a large amount of CH when they erode the periodontal tissue ₃ SH and H ₂ S。

The ingredients affecting halitosis are: hydrogen sulfide, methyl mercaptan, dimethyl sulfide, dimethyl disulfide, dimethyl trisulfide, amines, skatole, acetic acid, and the like.

The principle of the breath detector is as follows: when there is sufficient oxygen in the air, the free electrons in the sensor are attracted by the oxygen, and the current of the sensor is weak. When a reducing gas such as hydrogen sulfide is present in the air. The reaction of the reducing gas with oxygen reduces the free electrons in the oxygen concentration sensor to be released and the current flowing through the sensor increases. The specific condition of the breath composition is judged by a sensor.

3. Design of experimental protocol

Halitosis protocol design

The classification of halitosis mainly adopts the classification methods of Murata, etc., yaegaki and Coil to classify the halitosis into true halitosis, pseudo halitosis and halitosis phobia; the real halitosis can be divided into physiological halitosis and pathological halitosis, wherein the physiological halitosis can be considered as the cause of oral flora imbalance, and the pathological halitosis is the cause of oral diseases. Many bacteria in the oral cavity are able to utilize methionine, cysteine and serum to produce CH ₃ SH and H ₂ S, 4 bacteria (Porphyromonas gingivalis, treponema denticola, prevotella intermedia, fostanemia fosfomentalis) associated with periodontal disease showed more active sulfur-producing ability.

Therefore, no matter physiological halitosis and pathological halitosis, the oral cavity bacteria flora are directly related, so that the oral cavity bacteria flora is adjusted, the oral cavity immunity is improved, the oral cavity pathogenic bacteria are inhibited, the important means for controlling the oral cavity halitosis is provided, and the main purpose of the experiment is provided.

14 subjects were selected and confirmed to be genuine halitosis by sensory and communication inquiry. Meanwhile, factors which may influence experimental results, such as digestive system diseases, respiratory system diseases, diabetes, liver diseases and the like, are eliminated. The details are shown in Table 3 below.

TABLE 3

Periodontal patients	Gum patients	Physiological halitosis people	Total up to
				3	3	8	14

An intelligent breath detector (trade name: shownovo) is selected for the experiment.

The experimental method comprises the following steps: the subject rinsed with approximately 10ml of gargle of example 2 of the present invention three times a day in the morning, in the evening for 3 consecutive days; breath is detected 2 hours after each use, products are not used on days 4 and 5, detection is carried out twice every day, and the detection result is counted. The results are given in Table 4 below.

TABLE 4

And (4) experimental conclusion: the halitosis of all subjects is obviously improved; the improvement effect is most obvious after the first use, and the mouth smell is in a continuous descending trend after the continuous use for three days; after the patient was stopped from use on days 4 to 5, the breath level of some persons had a little rebound, but was also lower than the breath level before use. Experiments prove that the gargle for regulating oral flora and improving oral immunity has good use effects on regulating oral flora and reducing halitosis; meanwhile, the gargle provided by the invention has a certain synergistic effect on the repair of oral diseases, and three reasons can be analyzed: the product contains multiple antioxidant components, and can remove free radicals at focus, and relieve symptoms; part of active ingredients in the product can inhibit the oral pathogenic bacteria; the product improves the immunity of oral tissues and promotes the tissue repair.

Test example 2

Sensory experience experimental scheme design

10 oral health non-oral case persons are randomly selected, and the experimental subjects confirm that the oral cavity is not clean, the odor is bad breath and the like in different degrees through sensory and communication inquiry. The experimental method comprises the following steps: after the subject had a meal in the morning, at noon and evening, the gargle of example 2 was used, approximately 10ml each time, continuously; the use feeling is recorded after half an hour after each use, and the change of the oral cavity environment and the carrying degree are observed. The scoring criteria are shown in table 5 below.

TABLE 5

The sensory evaluation results are shown in Table 6 below.

TABLE 6

People ranking	Sweetness level	Taste of the product	Cleanliness	Degree of portability	Fresh breath	Scoring
							1	19	19	19	20	20	97
2	19	20	19	20	20	98
							3	20	19	19	20	20	98
4	19	19	18	20	20	96
							5	20	19	19	20	20	98
6	19	19	18	20	20	96
							7	20	20	18	20	20	98
8	20	19	18	20	20	97
							9	19	20	18	20	20	97
10	19	19	18	20	20	96

Remarking; a score above 90 is acceptable.

And (4) conclusion: all subjects have moderate sweetness in sensory perception, aromatic flavor, mouthfeel and aromatic flavor, are coordinated, have moderate mouthfeel, have no bitter and astringent taste, have no irritation, and are good for mouth feel. The sense of smell, taste and oral cavity is good. The test subject reaction strip is high in portability, convenient to carry and convenient to take along. Moreover, the cleanliness is also very high, and the oral cavity can be ensured to be clean without dirt and odor. And the menthol is contained in the formula, so that the oral environment and the oral odor are effectively improved, the halitosis can be inhibited, and the reaction experience of a subject is very good.

Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims

1. A gargle for improving the microenvironment of the oral cavity and enhancing the immunity of the oral cavity is characterized in that the gargle comprises carbomer, an antioxidant, a probiotic growth factor, vitamin D and purified water;

the antioxidant is selected from one or more of tea polyphenol, vitamin C ethyl ether and vitamin E;

the probiotic growth factor is selected from one or more of trehalose, isomaltooligosaccharide and fructo-oligosaccharide;

the viscosity value of the gargle is 100-400mpa.s.

2. The mouthrinse of claim 1, wherein the carbomer is carbomer 971P or carbomer 974P; based on the mass of the gargle, the mass percentage of the carbomer is 0.1-0.3%.

3. The mouthrinse of claim 1, wherein the antioxidant is present in an amount of from 0.05 to 0.3:0.05-0.3:0.1-2 of tea polyphenol, vitamin C ethyl ether and vitamin E.

4. The mouthrinse of claim 1, wherein the probiotic growth factor is present in an amount comprised of, by mass, 0.2-2:0.5-3:0.5-3 parts of trehalose, isomaltooligosaccharide and fructo-oligosaccharide.

5. The mouthrinse of claim 1, further comprising an additive selected from one or more of a sweetener, a humectant, a flavoring agent, an emulsifier, and a pH adjuster.

6. The mouthrinse of claim 5,

the sweetening agent is xylitol;

the humectant is glycerin;

the flavoring agent is eugenol or menthol;

the emulsifier is polyoxyethylene hydrogenated castor oil;

the pH regulator is potassium hydroxide.

7. The mouthrinse according to any one of claims 1 to 6, wherein the mouthrinse is prepared from the following raw materials in percentage by weight:

0.1-0.3% of carbomer, 0.05-0.3% of tea polyphenol, 0.05-0.3% of vitamin C ethyl ether, 0.1-2% of vitamin E, 0.2-2% of trehalose, 0.5-3% of isomaltooligosaccharide, 0.5-3% of fructo-oligosaccharide, 0.01-0.1% of vitamin D, 0.5-5% of xylitol, 1-10% of glycerol, 0.1-1.0% of eugenol, 0.05-0.5% of menthol, 0.5-3.0% of polyoxyethylene hydrogenated castor oil, a proper amount of potassium hydroxide, a pH value of 6.0-7.0 and the balance of purified water.

8. A process for the preparation of an oral rinse of claim 7 comprising the steps of:

(1) Preparing the raw materials according to the formula proportion;

(3) Adding vitamin C ethyl ether, vitamin E, vitamin D, eugenol and menthol into polyoxyethylene hydrogenated castor oil, uniformly stirring, adding a proper amount of purified water, and stirring until the materials are completely dissolved to obtain a material B;

9. The method according to claim 8, wherein, in the step (2),

after adding purified water, regulating the rotation speed to 300-1500rpm, continuously stirring for 1-5 hours, reducing the rotation speed to 100-300rpm when a large amount of bubbles are visible, stirring until the bubbles are obviously reduced, and when no lumps exist in the material, adding the stirring speed until the carbomer is completely dissolved.

10. The method according to claim 8, wherein in the step (3), the amount of the purified water is 2 to 3 times by volume as much as that of the polyoxyethylene hydrogenated castor oil.