CN115844015A - Lycopene oil, lycopene preparation and application thereof - Google Patents

Abstract

The invention relates to a lycopene oleogel, a lycopene preparation and application thereof. According to the invention, the lycopene oil gel contains 40% of lycopene, the particle size range of the lycopene oral preparation product is 200-400 nm, and the content of lycopene reaches 10-40 mg/mL, so that the problem of low content of lycopene in the prior art is solved. The product of the invention has the characteristics of small solution particle size, high bioavailability, stable product property and the like.

Description

A kind of lycopene oil, lycopene preparation and application thereof

technical field

The invention relates to food, health products, pharmaceutical raw materials and products, in particular to a lycopene oil gel, a lycopene preparation and applications thereof.

Background technique

Lycopene is a functional natural fat-soluble pigment, which has some physiological effects such as anti-oxidation and anti-inflammation. The Food and Agriculture Organization of the United Nations, the Committee on Food Additives and the World Health Organization have identified tomato red as a type A nutrient, and it has been widely used in health food, medicine, cosmetics, agriculture and other fields. Lycopene is a typical isoprene compound. Due to the existence of its unsaturated bonds, it is easily degraded and lost due to the influence of external adverse conditions. Therefore, lycopene is the main health care product on the market and often comes in soft capsules And tablet etc. are the main ways of existence.

Since lycopene is insoluble in water, slightly soluble in edible oil, the solubility in various organic solvents is also at a low level. The lack of effective food-grade solvents increases the difficulty of enriching lycopene, thus affecting the development of high-load dosage forms.

Emulsion is a carrier system that is uniformly dispersed in the form of droplets in another immiscible liquid. For a long time, food emulsions have been used in the delivery of nutrients to enhance their stability, control their release during digestion, and ultimately increase their bioavailability to living organisms, providing support for the development of functional foods with desirable health benefits and sensory properties. An easy way. CN201510759680.X discloses a plant protein/soybean polysaccharide nanoemulsion embedding natural fat-soluble pigments. After lycopene is dissolved in oil, it is added to the mixed solution of plant acid-soluble protein and soybean polysaccharide, and the nano-emulsion is processed by high-pressure micro-jet nanoemulsion. The homogenizer is used for homogenizing under the pressure of 300-800 bar for 1-3 minutes, and the homogenized emulsion is heated at 80-90° C. for 0.5-1 hour to obtain a plant protein/soybean polysaccharide nanoemulsion embedding lycopene. However, the amount of lycopene embedded in this method is limited, only reaching 0.04-0.08g/100ml. CN201811637738.3 discloses the use of maltodextrin and concentrated whey protein as wall materials, rapeseed oil as the carrier of oil-soluble nutrients, and materials such as soybean lecithin and sorbitol, which are averaged by a high-pressure homogenizer, and reused Nano-liposome liquid is prepared by high-pressure micro-fluidic equipment, and then dried by vacuum spray drying technology to obtain microencapsulated nano-preparation. CN202210090802.0 discloses using gum arabic, maltodextrin, and sodium alginate as wall materials to obtain the core material lycopene through supercritical fluid extraction, and then mixing the core material and wall materials in proportion to ultra-high pressure micro-jet flow, homogeneous Simultaneous realization of emulsification, preparation of lycopene microemulsion, and spray drying to obtain lycopene microcapsules. However, these methods all have the problem that the lycopene load of the product is low.

Contents of the invention

The purpose of the present invention is to overcome the deficiencies of the existing products, aiming to provide a high-concentration lycopene oleogel, a preparation method of a high-load lycopene preparation and its application.

The primary purpose of the present invention is to provide a high-concentration lycopene oleogel. The lycopene oleogel is under the protection of an inert gas, and the lycopene crystals are formed into molten lycopene by using a high-temperature autoclave. It is prepared by mixing molten lycopene and oil evenly.

In one aspect of the present invention, the present invention provides a lycopene oleogel product and a preparation method, the method comprising placing lycopene crystals in a vacuum autoclave under the protection of an inert gas, and maintaining a pressure of 0.08 to - 0.1Mpa, hold for 10-20 minutes to obtain molten lycopene, add oil and stir evenly at a uniform speed, and cool rapidly to obtain lycopene oil gel, which contains 40% lycopene.

In one aspect of the present invention, the present invention provides a lycopene preparation, the particle size range of lycopene in the preparation is 200-400 nm, and the content of lycopene is 10-20 mg/mL.

One aspect of the present invention, the present invention provides a kind of preparation method of lycopene preparation, described method comprises the steps:

(1) Preheat the water-soluble emulsifier with a mass percentage of 2%~8% to 90~95°C to obtain liquid A;

(1) (2) Put the lycopene oleogel and oil into a fixed container, pass in an inert gas for protection, raise the temperature of the container to 175-185°C, stir rapidly, and keep it for 30-60 s, and finally obtain B Liquid; Preferably, the grease and the grease used for preparing lycopene oleogel are the same grease;

(3) Quickly add liquid B to liquid A, emulsify through high-speed shearing, and obtain lycopene coarse emulsion;

(4) Pass the coarse emulsion obtained in step (3) through a high-pressure homogenizer to obtain a lycopene fine emulsion;

(5) Sterilize the lycopene microemulsion obtained in step (4) and store it in a refrigerator at 4°C to obtain a lycopene preparation.

Preferably, the high-speed shear emulsification in step (3) in the preparation method of the lycopene preparation of the present invention is 10000-12000 r/min, 3-5 min.

Preferably, the high-pressure homogenizer in the step (4) of the preparation method of the lycopene preparation of the present invention is 70-90 MPa, 3 cycles.

Preferably, the mass ratio of the oil phase to the water phase in the preparation method of the lycopene preparation of the present invention is 5:100 to 20:100; the mass ratio of the lycopene to the water phase is 1:100 to 4:100; the emulsifier and water The mass ratio is 2:100～8:100.

In one aspect of the present invention, the lycopene oleogel and the lycopene preparation of the present invention can be used in the preparation of food, medicine and health products. The lycopene preparation can be prepared as a liquid oral liquid preparation, and can also be further processed on the basis of the preparation, such as spray-dried to prepare a microcapsule powder. Of course, the corresponding foods, medicines, and health products containing lycopene oleogel and lycopene preparations of the present invention may also include auxiliary materials or components that are allowed to be added or used in foods and medicines.

Beneficial effect

In the present invention, under the protection of an inert gas, a high-concentration lycopene oleogel is prepared by using a vacuum autoclave, and then compounded with an emulsifier to prepare a stable lycopene oral preparation product. Among them, lycopene oleogel contains 40% lycopene, and the particle size range of lycopene oral preparations is 200-400 nm, and the lycopene content reaches 10-40 mg/mL. The product of the invention has the characteristics of small solution particle size, high bioavailability, stable product properties and the like.

Description of drawings

Fig. 1: The lycopene oleogel product of the present invention.

Figure 2: Particle size distribution of lycopene formulations according to the invention.

Fig. 3: Lycopene preparation product of the present invention.

Detailed ways

In order to better understand the present invention, the present invention will be further described below in conjunction with specific embodiments, but the examples do not limit the present invention in any form. Unless otherwise specified, the raw materials used in the examples of the present invention are conventionally purchased commercial raw materials.

Example 1

1.1 Preparation of lycopene oleogel

Under the protection of an inert gas, place lycopene crystals in a vacuum autoclave, keep the pressure at 0.08-0.1Mpa, and keep for 20 minutes to obtain molten lycopene, add canola oil and stir evenly at a uniform speed, and cool quickly to obtain The lycopene oleogel contains 40% lycopene.

1.2 Preparation of oral preparations of lycopene

(1) Preheat 95 mL of an emulsifier sodium sucrose fatty acid aqueous solution with a mass percentage of 2% to 90-95°C to obtain liquid A

(2) Mix 2.5 g lycopene oleogel and 2.5 g canola oil into a fixed container, raise the temperature to 175-185°C for 15 s under the protection of inert gas, and obtain liquid B.

(3) Liquid B was quickly added to liquid A, and emulsified by high-speed shear (10000 r/min, 3 min) to obtain lycopene coarse emulsion.

(4) Pass the coarse emulsion obtained in step (3) through a high-pressure homogenizer (70MPa, 3 cycles) to obtain a lycopene fine emulsion.

(5) Rapidly cooling the lycopene miniemulsion obtained in step (4) to obtain an oral preparation of lycopene. The content of lycopene is 10 mg/mL, and the average particle size of lycopene miniemulsion is 226 nm.

Example 2

2.1 Preparation of lycopene oleogel

Under the protection of an inert gas, place lycopene crystals in a vacuum autoclave, keep the pressure at 0.08-0.1Mpa, and keep for 20 minutes to obtain molten lycopene, add soybean oil and stir evenly at a uniform speed, and quickly cool to obtain tomato Lycopene oleogel, containing 40% lycopene in the lycopene oleogel.

2.2 Preparation of oral preparations of lycopene

(1) Preheat 90 mL of 4% sodium caseinate aqueous solution to 90-95°C to obtain liquid A

(2) Mix 5 g of lycopene oleogel and 5 g of soybean oil into a fixed container, raise the temperature to 175-185°C for 30 s under the protection of an inert gas, and obtain liquid B.

(3) Liquid B was quickly added to liquid A, and subjected to high-speed shear emulsification (10000 r/min, 3.5 min) to obtain a lycopene coarse emulsion.

(4) Pass the coarse emulsion obtained in step (3) through a high-pressure homogenizer (70MPa, 3 cycles) to obtain a lycopene fine emulsion.

(5) The lycopene microemulsion obtained in step (4) is sterilized and stored at 4°C to obtain an oral lycopene preparation. The content of lycopene is 20 mg/mL, and the average particle size of lycopene miniemulsion is 262 nm.

Example 3

3.1 Preparation of lycopene oleogel

Under the protection of an inert gas, place lycopene crystals in a vacuum autoclave, keep the pressure at 0.08-0.1Mpa, and keep for 20 minutes to obtain molten lycopene, add corn oil and stir evenly at a uniform speed, and quickly cool to obtain lycopene Vegetarian oil gel, containing 40% lycopene in the said lycopene oil gel.

3.2 Preparation of oral preparations of lycopene

(1) Preheat 85 mL of 6% polyglycerol fatty acid ester aqueous solution to 90-95°C to obtain liquid A

(2) Mix 7.5 g lycopene oleogel and 7.5 g corn oil into a fixed container, raise the temperature to 175-185°C for 45 s under the protection of inert gas, and obtain liquid B.

(3) Liquid B was quickly added to liquid A, and emulsified by high-speed shearing (120000 r/min, 4 min) to obtain lycopene coarse emulsion.

(4) Pass the coarse emulsion obtained in step (3) through a high-pressure homogenizer (80MPa, 3 cycles) to obtain a lycopene fine emulsion.

(5) Sterilize the lycopene microemulsion obtained in step (4) and store it in a refrigerator at 4°C to obtain an oral lycopene preparation. The content of lycopene is 30 mg/mL, and the average particle size of lycopene miniemulsion is 312 nm.

Example 4

3.1 Preparation of lycopene oleogel

Under the protection of an inert gas, place lycopene crystals in a vacuum autoclave, keep the pressure at 0.08-0.1Mpa, and keep for 20 minutes to obtain molten lycopene, add canola oil and stir evenly at a uniform speed, and cool quickly to obtain The lycopene oleogel contains 40% lycopene.

3.2 Preparation of oral preparations of lycopene

(1) Preheat 80 mL of 8% sucrose fatty acid sodium aqueous solution to 90-95°C to obtain liquid A

(2) Mix 10 g lycopene oleogel and 10 g canola oil into a fixed container, raise the temperature to 175-185°C for 60 s under the protection of inert gas, and obtain liquid B.

(3) Liquid B was quickly added to liquid A, and emulsified by high-speed shear (12000 r/min, 5 min) to obtain a lycopene coarse emulsion.

(4) Pass the coarse emulsion obtained in step (3) through a high-pressure homogenizer (90MPa, 3 cycles) to obtain a lycopene fine emulsion.

(5) Sterilize the lycopene microemulsion obtained in step (4) and store it in a refrigerator at 4°C to obtain an oral lycopene preparation. The content of lycopene is 40 mg/mL, and the average particle size of lycopene miniemulsion is 345 nm.

The above content is a further detailed description of the present invention in conjunction with specific embodiments. It cannot be determined that the specific implementation of the present invention is limited to these descriptions. For those of ordinary skill in the technical field of the present invention, without departing from the concept of the present invention. Several simple inferences or substitutions can also be made, all of which should be deemed to belong to the protection scope determined by the claims submitted in the present invention.

Claims (10)

1. A lycopene oleogel, characterized in that, the lycopene is under the protection of an inert gas, and the lycopene crystals are processed by a vacuum autoclave to form a molten lycopene, and the lycopene is added and stirred evenly at a uniform speed Rapid cooling to obtain a stable and uniform lycopene oil gel. 2. The preparation method of lycopene oleogel according to claim 1, characterized in that, the method comprises placing lycopene crystals in a vacuum autoclave under the protection of an inert gas, and maintaining a pressure of 0.08-- 0.1 Mpa, hold for 10-20 min to obtain molten lycopene, add oil and stir evenly at a uniform speed, and cool rapidly to obtain lycopene oleogel, which contains 40% lycopene. 3. A lycopene preparation, characterized in that the particle size of lycopene in the preparation ranges from 200 to 400 nm, and the content of lycopene is 10 to 40 mg/mL. 4. according to the preparation method of the described lycopene preparation of claim 3, it is characterized in that, described method comprises the steps: Preheat the water-soluble emulsifier with a mass percentage of 2%~8% to 90~95°C to obtain liquid A; Put lycopene oleogel and oil into a fixed container, pass in an inert gas for protection, raise the temperature of the container to 175-185°C, stir rapidly, and keep it for 30-60 s, and finally obtain liquid B; preferably, the Said grease is the same grease as the grease used to prepare lycopene oleogel; Quickly add liquid B to liquid A, emulsify through high-speed shearing, and obtain lycopene coarse emulsion; The coarse emulsion obtained in step (3) is subjected to high-pressure dynamic micro-fluidization to obtain lycopene fine emulsion; The lycopene microemulsion obtained in step (4) was sterilized and stored in a refrigerator at 4°C to obtain a lycopene preparation. 5. The method according to claim 4, wherein the water-soluble emulsifier in the step (1) is selected from mono- and diglyceride fatty acid esters and derivatives thereof, polyglycerol fatty acid esters and derivatives thereof Phospholipids and their derivatives, modified phospholipids, sodium caseinate, soybean protein isolate, starch, modified starch, monoglycerides of fatty acids, sucrose fatty acid esters, sorbitan fatty acid esters, propylene glycol fatty acid esters one or more of . 6. according to the described method of claim 2 or 4, it is characterized in that, described grease is medium-chain fatty acid triglycerides, corn oil, soybean oil, olive oil, sunflower seed oil, canola oil, sesame oil one or more. 7. The method according to claim 4, characterized in that the high-speed shear emulsification in the step (3) is 10000-12000 r/min, 3-5 min. 8 . The method according to claim 4 , characterized in that, the high-pressure dynamic micro-jet in the step (4) is 70-90 MPa, 3 cycles. 9. The method according to claim 4, characterized in that, the mass ratio of the oil phase to the water phase of the method is 5:100～20:100; the mass ratio of lycopene to the water phase is 1:100～4 :100; the mass ratio of emulsifier to water is 2:100～8:100. 10. The application of the lycopene oleogel according to claim 1 and the lycopene preparation according to claim 3 in the preparation of food, medicine and health products.

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