CN115808421A - Agglutination state interpretation method - Google Patents
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Abstract
The invention provides an agglutination state interpretation method, wherein in the process that each layer of liquid storage layer is spanned by the thickness of parallel light, a camera is used for collecting two-dimensional overlook images of a plurality of frames of receiving pieces, and parts, aligned with a reaction cavity, in the two-dimensional overlook images are extracted and subjected to rasterization processing to obtain a plurality of two-dimensional visual units; acquiring a brightness detection value of each two-dimensional visual unit; analyzing the brightness detection value of each two-dimensional visual unit by taking a frame as a unit so as to judge whether the mixed solution is agglutinated or not; and if the mixed liquor is judged to be agglutinated, sequentially determining the brightness value data of each three-dimensional unit layer by taking the layer as a unit, respectively confirming each three-dimensional unit as an agglutinating point or a dispersing point according to the brightness value data of each three-dimensional unit, and judging the agglutinating state of the mixed liquor according to the number and the position of the confirmed agglutinating points. The invention not only can determine whether the mixed solution is agglutinated, but also can provide additional reference for the detection of the blood type antibody concentration of the sample.
Description
Technical Field
The invention relates to the field of biochemical detection, in particular to an agglutination state interpretation method.
Background
In the field of biochemical detection, a sample to be detected is often added to a selected reagent, and according to a change of the reagent added to the sample, an attribute of the sample, such as a blood type identification test, is obtained. However, the conventional blood grouping method can only determine whether there is any agglutination in the mixed solution, but cannot further know the agglutination states such as the number and the specification of the agglutination, and in fact, the blood group antibody concentration of the sample affects the agglutination state of the agglutination, for example, if the blood group antibody concentration of the sample is high, the mixed solution of the sample and the reagent is agglutinated, the number of the agglutination in the mixed solution is high, and the specification of the individual agglutination is also high. Thus, if the agglutination state of the mixed solution can be interpreted, not only can whether the mixed solution agglutinates be determined, but also an additional reference can be provided for the blood group antibody concentration detection of the sample.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides an agglutination state interpretation method, which can interpret the agglutination state of the mixed solution.
The invention adopts the following technical scheme.
A method of agglutination status interpretation, the method comprising:
establishing a reaction cavity model of a containing part according to a proportion, wherein the containing part is of a transparent cylindrical structure, and a mixed solution of a sample and a reagent is stored in a reaction cavity of the containing part;
uniformly dividing the reaction cavity model into a plurality of liquid storage layers along the vertical direction, and rasterizing the liquid storage layers along the horizontal direction to obtain a plurality of three-dimensional units;
the method comprises the following steps that parallel light generated by a light source is utilized to irradiate a containing part along the horizontal direction, the liquid storage layer at the topmost layer is taken as a starting point, the thickness of the parallel light is extended downwards, the thickness of the parallel light sequentially spans each liquid storage layer, the light path of the parallel light is perpendicular to one end, close to the light source, of a three-dimensional unit, and the width of the parallel light always spans the projection of each liquid storage layer on the width of the parallel light;
in the process that each layer of liquid storage layer is spanned by the thickness of parallel light, a camera is used for collecting two-dimensional overhead images of a plurality of frames of receiving pieces, the part of the two-dimensional overhead images, which is aligned with the reaction cavity, is extracted and is subjected to rasterization processing, and a plurality of two-dimensional visual units are obtained;
acquiring a brightness detection value of each two-dimensional visual unit;
analyzing the brightness detection value of each two-dimensional visual unit by taking a frame as a unit so as to judge whether the mixed liquid is agglutinated or not;
and if the mixed liquor is judged to be agglutinated, sequentially determining the brightness value data of each three-dimensional unit layer by taking the layer as a unit, respectively determining each three-dimensional unit as an agglutinating point or a dispersing point according to the brightness value data of the three-dimensional unit, and judging the agglutinating state of the mixed liquor according to the number and the position of the determined agglutinating points.
Further, the specification of the rasterization processing of each layer of liquid storage layer is consistent with the specification of the rasterization processing of each frame of two-dimensional overhead image.
Further, analyzing the brightness detection value of each two-dimensional visual unit in units of frames includes:
the average brightness value of the two-dimensional overhead image of each frame is obtained by the following steps:
in the formula:
n is the number of two-dimensional visual units of the jth frame of the two-dimensional overhead view image;
Further, analyzing the luminance detection values of the respective two-dimensional visual units in units of frames, further includes:
respectively obtaining the difference between the brightness detection value of each two-dimensional visual unit and the brightness average value of the two-dimensional overhead image to which the two-dimensional visual unit belongs;
if the absolute value of each difference is smaller than the set threshold, judging that the mixed solution is not agglutinated;
if the absolute value of the difference is larger than the set threshold, the mixed liquid is judged to be agglutinated.
Further, the brightness value data includes a brightness measurement value, a brightness calibration value, and a brightness actual value.
Further, the brightness measurement value of each three-dimensional stereo unit conforms to the following formula:
in the formula:
m is the number of two-dimensional overlooking images from the t-th layer of liquid storage layer;
and the ith three-dimensional unit of the tth liquid storage layer and the ith two-dimensional visual unit of each frame of two-dimensional overhead image from the tth liquid storage layer both accord with a position mapping relation.
Further, the brightness calibration value of any three-dimensional stereo unit of any liquid storage layer except the topmost liquid storage layer accords with the following formula:
in the formula:
the liquid storage layer of the (t-1) th layer is an upper layer adjacent to the liquid storage layer of the t-th layer, and the ith three-dimensional unit of the liquid storage layer of the t-th layer is aligned with the ith three-dimensional unit of the liquid storage layer of the (t-1) th layer;
the brightness calibration value of each three-dimensional stereo unit of the topmost liquid storage layer is equal to the respective brightness measurement value.
Further, the actual brightness value of any three-dimensional unit of any liquid storage layer except the topmost liquid storage layer conforms to the following formula:
in the formula:
x is the number of the condensation points which are positioned above the ith three-dimensional unit of the t-th layer of liquid storage layer and accord with the position mapping relationship with the three-dimensional unit;
y is the number of dispersed points which are positioned above the ith three-dimensional unit of the t-th layer of liquid storage layer and accord with the position mapping relationship with the three-dimensional unit;
and the actual brightness value of each three-dimensional unit of the topmost liquid storage layer is equal to the respective brightness calibration value.
Further, a two-dimensional coordinate system is established according to the position of the three-dimensional stereo unit on the light path and the actual brightness value of the three-dimensional stereo unit, a condensation point scattered light intensity attenuation line and a dispersion point scattered light intensity attenuation line are established in the two-dimensional coordinate system, and the three-dimensional stereo unit with the determined actual brightness value is substituted into the two-dimensional coordinate system according to the position of the three-dimensional stereo unit on the light path;
confirming a three-dimensional unit positioned outside the scattered light intensity attenuation line of the condensation point as a condensation point;
three-dimensional stereo cells located outside the scattered light intensity attenuation line of the dispersed point were confirmed as dispersed points.
Further, the interpretation of the agglutination state of the mixed solution includes:
according to the number of the coagulation points existing at the adjacent positions of the coagulation points, respectively endowing the coagulation points with weight values, combining a plurality of the coagulation points which are sequentially connected and have the weight values larger than a preset value into a mass center, combining the rest coagulation points with the mass centers respectively connected into coagulation groups, and calculating the number of the coagulation groups and the number of the coagulation points contained in each coagulation group.
The beneficial effects of the invention are as follows:
based on the Tyndall effect, for any three-dimensional unit, in the process that a liquid storage layer to which the three-dimensional unit belongs is crossed by the thickness of parallel light, if the three-dimensional unit is aggregated, the intensity of scattered light generated by the three-dimensional unit is greater than the intensity of scattered light generated when the three-dimensional unit is not aggregated, and correspondingly, the brightness detection value of a two-dimensional visual unit derived from the three-dimensional unit is also greater than the brightness detection value when the three-dimensional unit is not aggregated; then, acquiring a brightness detection value of each two-dimensional visual unit; then, analyzing the brightness detection value of each two-dimensional visual unit by taking a frame as a unit so as to judge whether the mixed liquid is agglutinated or not; and if the mixed liquor is judged to be agglutinated, sequentially determining the brightness value data of each three-dimensional unit layer by taking the layer as a unit, respectively confirming each three-dimensional unit as an agglutinating point or a dispersing point according to the brightness value data of each three-dimensional unit, and judging the agglutinating state of the mixed liquor according to the number and the position of the confirmed agglutinating points.
In summary, the present invention can interpret the agglutination status of the mixed solution, not only can determine whether the mixed solution is agglutinated, but also can provide an additional reference for the blood group antibody concentration detection of the sample.
Drawings
In order to more clearly illustrate the embodiments or prior art solutions of the present invention, the drawings used in the description of the embodiments or prior art will be briefly described below, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a flow chart of an agglutination status interpretation method according to the present embodiment;
fig. 2 is a schematic diagram of a hardware structure provided in this embodiment;
fig. 3 is a schematic diagram of analyzing a luminance detection value of a two-dimensional visual unit according to this embodiment;
FIG. 4 is a second schematic diagram illustrating the analysis of the brightness detection value of the two-dimensional visual unit according to the present embodiment;
fig. 5 is a schematic diagram of obtaining a luminance measurement value of a three-dimensional stereo unit according to the present embodiment;
fig. 6 is a schematic diagram of obtaining a brightness calibration value of a three-dimensional stereo unit according to the present embodiment;
fig. 7 is a schematic diagram of calculating an actual brightness value of a three-dimensional stereo unit according to this embodiment;
FIG. 8 is a schematic diagram of the present embodiment for identifying a three-dimensional stereo unit as a condensation point or a dispersion point;
FIG. 9 is a schematic view showing the judgment of the aggregation state of the mixed solution according to the present example.
Description of reference numerals:
the device comprises a container 1, a light emitting member 21, a parallel light lens 22, a shading plate 23, a rotary actuating member 31, a primary pulley 32, a transmission belt 33, a secondary pulley 34, a position sensor 4 and a camera 5.
Detailed Description
The drawings are for illustrative purposes only and are not to be construed as limiting the patent; for a better understanding of the present embodiments, certain features of the drawings may be omitted, enlarged or reduced, and do not represent actual product dimensions.
It will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted. The technical solution of the present invention is further described with reference to the drawings and the embodiments.
Referring to fig. 1, fig. 1 is a flow chart of an agglutination status interpretation method according to the present invention.
The invention provides an agglutination state interpretation method, which comprises the following steps:
establishing a reaction cavity model of a containing part 1 according to a proportion, wherein the containing part 1 is of a transparent cylindrical structure, and a mixed solution of a sample and a reagent is stored in the reaction cavity of the containing part 1; it can be understood that, to facilitate the establishment of the reaction chamber model, the receptacle 1 is preferably a square cylindrical structure;
uniformly dividing the reaction cavity model into a plurality of liquid storage layers along the vertical direction, and carrying out rasterization treatment on each liquid storage layer along the horizontal direction to obtain a plurality of three-dimensional units;
the method comprises the following steps that parallel light generated by a light source is utilized to irradiate a containing part 1 along the horizontal direction, the liquid storage layer at the topmost layer is taken as a starting point, the thickness of the parallel light extends downwards, the thickness of the parallel light sequentially spans each liquid storage layer, the light path of the parallel light is perpendicular to one end, close to the light source, of a three-dimensional unit, and the width of the parallel light always spans the projection of each liquid storage layer on the width of the parallel light;
as an example, as shown in fig. 2, the light source includes a light emitting member 21 having a bar shape and extending in a vertical direction, a parallel light lens 22 disposed between the light emitting member 21 and the accommodating member 1, and a light shielding plate 23 disposed between the light emitting member 21 and the parallel light lens 22, wherein the light shielding plate 23 is connected to a lifting actuating mechanism, and the lifting actuating mechanism can drive the light shielding plate 23 to move in the vertical direction, so that the thickness of the parallel light generated by the light emitting member 21 sequentially spans each layer of liquid storage layer downward. In addition, the present example is further provided with a position sensor 4 acting on the light shielding plate 23, and the position sensor 4 can sense the position of the light shielding plate 23, so that for any two-dimensional overhead image, according to the liquid storage layer aligned with the top end of the light shielding plate 23 when the camera 5 acquires the two-dimensional overhead image, the liquid storage layer from which the two-dimensional overhead image originates, that is, the two-dimensional overhead image is the two-dimensional overhead image originating from the liquid storage layer aligned with the top end of the light shielding plate 23. The light emitting member 21 of this example can emit light uniformly, and the light intensities received by the liquid storage layers are made equal.
As an example, the lifting actuating mechanism includes a rotary actuating member 31, a primary pulley 32 connected to the rotary actuating member 31, and a secondary pulley 34 connected to the primary pulley 32 via a transmission belt 33, wherein the light shielding plate 23 is connected to the transmission belt 33, so that the rotary actuating member 31 can move the light shielding plate 23 in a vertical direction during rotation of the primary pulley 32.
In the process that each layer of liquid storage layer is spanned by the thickness of parallel light, a camera 5 is used for collecting two-dimensional overhead images of a plurality of frames of receiving pieces 1, the parts of the two-dimensional overhead images, which are aligned with the reaction cavity, are extracted and subjected to rasterization treatment, and a plurality of two-dimensional visual units are obtained; it should be noted that, for an arbitrary liquid storage layer, a plurality of frames of two-dimensional top-view images collected during the process of crossing the liquid storage layer by the parallel light are two-dimensional top-view images derived from the liquid storage layer, and in addition, a two-dimensional visual unit belonging to each frame of two-dimensional top-view image and conforming to a position mapping relationship with an arbitrary three-dimensional unit of the liquid storage layer is a two-dimensional visual unit derived from the three-dimensional unit.
The specification of the rasterization processing of each layer of liquid storage layer is consistent with the specification of the rasterization processing of each frame of two-dimensional overhead view image, and in the present example, the rasterization processing is performed on each layer of liquid storage layer and each frame of two-dimensional overhead view image according to the specification of the pixel point of the camera 5, so that each three-dimensional unit of each layer of liquid storage layer and each two-dimensional visual unit of each frame of two-dimensional overhead view image respectively accord with the position mapping relationship one by one.
Acquiring a brightness detection value of each two-dimensional visual unit;
analyzing the brightness detection value of each two-dimensional visual unit by taking a frame as a unit so as to judge whether the mixed solution is agglutinated or not;
preferably, the analyzing the brightness detection values of the respective two-dimensional visual units in units of frames includes:
the average brightness value of each frame of the two-dimensional overhead image is obtained by:
in the formula:
n is the number of two-dimensional visual units of the jth frame of the two-dimensional overhead view image;
Preferably, the analyzing the luminance detection values of the respective two-dimensional visual units in units of frames further includes:
respectively obtaining the difference value between the brightness detection value of each two-dimensional visual unit and the brightness average value of the two-dimensional overhead image to which the two-dimensional visual unit belongs;
if the absolute value of each difference is smaller than the set threshold value, judging that the mixed solution is not agglutinated;
if the absolute value of the difference is larger than the set threshold value, the mixed liquid is judged to be agglutinated.
In this example, the threshold value is set to 1/10 of the absolute value of the difference between the intensity of scattered light when agglutination occurs and the intensity of scattered light when agglutination does not occur in the three-dimensional volume cell closest to the light source, and the set threshold value can be derived through a plurality of experiments. As an example, the set threshold is 5.
If the mixed liquid is not agglutinated, the sample and the reagent are uniformly dispersed in the reaction cavity, in this case, in the process that any liquid storage layer is spanned by the thickness of the parallel light, the scattered light intensity generated by each three-dimensional stereo unit on the liquid storage layer is relatively close, so that the brightness detection value of each two-dimensional visual unit of each two-dimensional overhead image of each frame of the liquid storage layer is relatively close, and the absolute value of the difference value between the brightness detection value of each two-dimensional visual unit of each two-dimensional overhead image of each frame of the liquid storage layer and the brightness average value of the two-dimensional overhead image of the two-dimensional visual unit is smaller than the set threshold value.
As an example, as shown in fig. 3, the liquid storage layer is provided with 3 × 3 three-dimensional stereoscopic cells, the number of the two-dimensional overhead images derived from the liquid storage layer is 1, and in view of the fact that the specification of the rasterization process of each liquid storage layer is consistent with the specification of the rasterization process of each frame of two-dimensional overhead image, the two-dimensional overhead images derived from the liquid storage layer are also provided with 3 × 3 two-dimensional visual cells, the luminance detection values of the 9 two-dimensional visual cells are respectively 26, 23, 21, 27, 23, 20, 26, 22, and 20, so that the average luminance value of the two-dimensional overhead images is 23.1, and the absolute value of the difference between the luminance detection values of the 9 two-dimensional visual cells and the average luminance value of the two-dimensional overhead images is less than 5, so as to determine that the liquid storage layer is not agglomerated.
Referring to the above principle, if the absolute value of the difference between the brightness detection value of each two-dimensional visual unit and the brightness average value of the two-dimensional overhead image to which each two-dimensional visual unit belongs is smaller than the set threshold, it indicates that no aggregation has occurred in each three-dimensional stereoscopic unit of each liquid storage layer, and thus, it indicates that no aggregation has occurred in the mixed liquid.
On the contrary, if any liquid storage layer is aggregated, in the process that the liquid storage layer is crossed by the thickness of the parallel light, the scattered light intensity of the three-dimensional stereo unit of the liquid storage layer where the aggregation occurs is larger than that of the three-dimensional stereo unit where the aggregation does not occur, and in addition, because the probability that each three-dimensional stereo unit of the liquid storage layer is aggregated is extremely low, the difference of the scattered light intensities generated by the respective three-dimensional stereo units on the liquid storage layer is larger, and thus, the difference of the brightness detection values of the respective two-dimensional visual units of each frame of two-dimensional top view images derived from the liquid storage layer is larger, so that the absolute value of the difference between the brightness detection value of each two-dimensional visual unit of each frame of two-dimensional top view images derived from the liquid storage layer and the brightness average value of the two-dimensional top view images to which the two-dimensional visual units belong is larger than the set threshold value.
As an example, as shown in fig. 4, the liquid storage layer is provided with 3 × 3 three-dimensional stereoscopic units, the number of the two-dimensional overhead images derived from the liquid storage layer is 1, and in view of the fact that the specification of the rasterization process of each liquid storage layer is consistent with the specification of the rasterization process of each frame of two-dimensional overhead image, the two-dimensional overhead images derived from the liquid storage layer are also provided with 3 × 3 two-dimensional visual units, and the brightness detection values of the 9 two-dimensional visual units are respectively 26, 60, 17, 61, 51, 13, 59, 18, and 13, so that the average brightness value of the two-dimensional overhead images is not 35.3, and the absolute value of the difference between the brightness detection values of the 9 two-dimensional visual units and the average brightness value of the two-dimensional overhead images is greater than 5, so that it is determined that the liquid storage layer is not agglutinated, and the mixed liquid is agglutinated.
It can be understood that the accuracy of the determination of the aggregation state is improved as the number of three-dimensional cells increases, and in other examples, the number of three-dimensional cells of each liquid storage layer is 50 × 50, 100 × 100, 300 × 300, and the like.
And if the mixed liquor is judged to be agglutinated, sequentially determining the brightness value data of each three-dimensional unit layer by taking the layer as a unit, wherein the brightness value data comprises a brightness measurement value, a brightness calibration value and a brightness actual value, respectively determining each three-dimensional unit as an agglutinating point or a dispersing point according to the brightness value data of the three-dimensional unit, and judging the agglutination state of the mixed liquor according to the determined number and position of the agglutinating points.
It can be understood that, for any liquid storage layer except for the topmost layer, the brightness value data of the liquid storage layer of the layer is determined after the brightness value data of the liquid storage layer of the layer is determined and the three-dimensional stereo units of the liquid storage layer of the layer are respectively confirmed as the condensation point or the dispersion point according to the brightness value data of the liquid storage layer of the layer.
Preferably, the brightness measurement value of each three-dimensional stereo unit conforms to the following formula:
in the formula:
m is the number of two-dimensional overlooking images from the t-th layer of liquid storage layer;
and the ith three-dimensional unit of the tth liquid storage layer and the ith two-dimensional visual unit of each frame of two-dimensional overhead image from the tth liquid storage layer both accord with a position mapping relation.
As an example, as shown in fig. 5, in the process that the upper, middle and lower parts of the liquid storage layer are spanned by the thickness of the parallel light, the kth light is collected by the camera 5 1 、k 2 、k 3 Frame a two-dimensional top view image, such that the number of two-dimensional top view images from the layer of liquid storage layer is 3, wherein 1 Frame, kth 2 Frame, kth 3 Ith of frame two-dimensional overlook image 1 Of a two-dimensional visual unitThe luminance detection values are 65, 66, 69, respectively, and are then compared with the k-th luminance detection values 1 Frame, kth 2 Frame, kth 3 Ith of frame two-dimensional overlook image 1 The ith two-dimensional visual unit conforms to the position mapping relation of the liquid storage layer 1 The brightness measurement for each three-dimensional volumetric cell was 66.7.
Preferably, for any liquid storage layer except the topmost liquid storage layer, the scattered light generated by any three-dimensional stereo unit of the liquid storage layer is superposed with the scattered light generated by another three-dimensional stereo unit which is positioned above the three-dimensional stereo unit and aligned with the three-dimensional stereo unit, so that the brightness detection value of the two-dimensional visual unit originated from the three-dimensional stereo unit is greater than the intensity of the scattered light actually generated by the three-dimensional stereo unit, and the brightness measurement value of the three-dimensional stereo unit is greater than the intensity of the scattered light actually generated by the three-dimensional stereo unit, and therefore, in order to eliminate the influence of another three-dimensional stereo unit on the intensity of the scattered light generated by the three-dimensional stereo unit, the brightness calibration value of any three-dimensional stereo unit of any liquid storage layer except the topmost liquid storage layer needs to be calibrated according to the following formula:
in the formula:
the liquid storage layer of the (t-1) th layer is an upper layer adjacent to the liquid storage layer of the t-th layer, and the ith three-dimensional unit of the liquid storage layer of the t-th layer is aligned with the ith three-dimensional unit of the liquid storage layer of the (t-1) th layer;
it can be understood that, in view of the fact that only air exists between the topmost liquid storage layer and the camera 5, and no other liquid storage layer exists, the luminance calibration values of the respective three-dimensional stereoscopic units of the topmost liquid storage layer are equal to the respective luminance measurement values.
As an example, as shown in FIG. 6, the t-th 1 Ith of liquid storage layer 1 The brightness measurement of each three-dimensional stereo cell was 66.7, th (t) 1 -1) th layer of liquid storage layer 1 The brightness measurement of each three-dimensional stereo unit is 57.2, th 1 Ith of liquid storage layer 1 The brightness calibration value of each three-dimensional stereo unit is 11.2.
Preferably, for any liquid storage layer except the topmost liquid storage layer, the scattered light generated by any three-dimensional stereo unit of the liquid storage layer is absorbed by another three-dimensional stereo unit which is positioned above the three-dimensional stereo unit and aligned with the three-dimensional stereo unit, so that the brightness calibration value of the three-dimensional stereo unit is smaller than the intensity of the scattered light actually generated by the three-dimensional stereo unit, and therefore, in order to eliminate the influence of the other three-dimensional stereo unit on the intensity of the scattered light generated by the three-dimensional stereo unit, the brightness calibration value of the three-dimensional stereo unit needs to be compensated, and in view of this, the actual brightness value of any three-dimensional stereo unit of any liquid storage layer except the topmost liquid storage layer conforms to the following formula:
in the formula:
x is the number of the condensation points which are positioned above the ith three-dimensional unit of the t-th liquid storage layer and conform to the position mapping relation with the three-dimensional unit;
y is the number of dispersed points which are positioned above the ith three-dimensional unit of the t-th layer of liquid storage layer and accord with the position mapping relationship with the three-dimensional unit;
the set light absorption value of the condensation point and the set light absorption value of the dispersion point can be obtained by derivation after multiple experiments; as an example, the set absorbance at the condensation point is 20 and the set absorbance at the dispersion point is 3.
As an example, as shown in FIG. 7, the t-th 1 Ith of liquid storage layer 1 The luminance calibration value of each three-dimensional solid cell is 11.2, the number of aggregation points located above the three-dimensional solid cell and aligned with the three-dimensional solid symbol is 2, the number of scattering points located above the three-dimensional solid cell and aligned with the three-dimensional solid symbol is 3, and the actual luminance value of the three-dimensional solid cell is 60.2.
It can be understood that, considering that only air exists between the topmost liquid storage layer and the camera 5, and no other liquid storage layer exists, the actual brightness value of each three-dimensional stereo unit of the topmost liquid storage layer is equal to the respective brightness calibration value.
Preferably, a two-dimensional coordinate system is established by the position of the three-dimensional stereo unit on the light path and the actual brightness value of the three-dimensional stereo unit, a condensation point scattered light intensity attenuation line and a dispersion point scattered light intensity attenuation line are established in the two-dimensional coordinate system, and the three-dimensional stereo unit with the determined actual brightness value is substituted into the two-dimensional coordinate system according to the position of the three-dimensional stereo unit on the light path;
confirming a three-dimensional unit positioned outside the scattered light intensity attenuation line of the condensation point as a condensation point;
three-dimensional stereo cells located outside the scattered light intensity attenuation line of the dispersed point were confirmed as dispersed points.
It should be noted that, the condensation point scattered light intensity attenuation line indicates that, if the light intensity of the light source is a first predetermined value, each three-dimensional unit on any optical path is assumed to be a condensation point, the relationship between the actual value of the brightness of each three-dimensional unit on the optical path and the position of each three-dimensional unit on the optical path is assumed, and the first predetermined value is smaller than the actual value of the light intensity of the light source, so that, for any condensation point, whether each three-dimensional unit located before the condensation point is also a condensation point or a three-dimensional unit located before the condensation point is a dispersion point, after the condensation point is substituted into the established two-dimensional coordinate system, the condensation point is located outside the condensation point scattered light intensity attenuation line. It can be understood that if a three-dimensional volume unit as a dispersion point exists before the agglutination point, the intensity of light actually received by the agglutination point is greater than the intensity of light expected to be received by the agglutination point when each three-dimensional volume unit before the agglutination point is an agglutination point, and correspondingly, the intensity of scattered light actually generated by the agglutination point is greater than the intensity of scattered light expected to be generated by the agglutination point when each three-dimensional volume unit before the agglutination point is an agglutination point, so that the agglutination point is positioned outside the attenuation line of the scattered light intensity of the agglutination point.
It should be noted that the scattered light intensity attenuation line at a scattering point indicates that, if the light intensity of the light source is a second predetermined value, assuming that each three-dimensional stereo unit on any optical path is a scattering point, the relationship between the actual brightness value of each three-dimensional stereo unit on the optical path and the position of each three-dimensional stereo unit on the optical path is, and the second predetermined value is greater than the actual light intensity value of the light source, so that, for any scattering point, whether each three-dimensional stereo unit located before the scattering point is also a scattering point, or a three-dimensional stereo unit existing as a condensation point before the scattering point, after the scattering point is substituted into the established two-dimensional coordinate system, the scattering point is located outside the scattered light intensity attenuation line at the scattering point. It can be understood that if a three-dimensional volume unit serving as a condensation point exists before the dispersion point, the intensity of light actually received by the dispersion point is smaller than the intensity of light expected to be received by the dispersion point when each three-dimensional volume unit before the dispersion point is a dispersion point, and accordingly, the intensity of scattered light actually generated by the dispersion point is smaller than the intensity of scattered light expected to be generated by the dispersion point when each three-dimensional volume unit before the dispersion point is a dispersion point, so that the dispersion point is located outside the attenuation line of the scattered light intensity of the dispersion point.
Wherein, the condensation point scattered light intensity attenuation line and the dispersion point scattered light intensity attenuation line can be obtained by derivation after a plurality of experiments.
As an example, as shown in FIG. 8, a three-dimensional solid unit a is arranged on any optical path 11 、a 12 、a 13 Establishing a two-dimensional coordinate system, wherein the position of the three-dimensional unit on the light path is used as an abscissa, the actual brightness value of the three-dimensional unit is used as an ordinate, a condensation point scattered light intensity attenuation line and a dispersion point scattered light intensity attenuation line are established in the two-dimensional coordinate system, and each three-dimensional unit on the light path is substituted into the two-dimensional coordinate system, wherein the three-dimensional unit a 11 、a 12 A three-dimensional unit a positioned outside the scattered light intensity attenuation line of the agglutination point 3 Is positioned outside the attenuation line of scattered light intensity of the dispersed point, and then a three-dimensional stereo unit a is formed 11 、a 12 Confirming as a condensation point, and placing the three-dimensional stereo unit a 13 The dot was confirmed as a scatter dot.
Preferably, the reading of the agglutination state of the mixed solution includes:
according to the number of the condensation points stored at the adjacent positions of the condensation points, a weight value is respectively given to the condensation points, a plurality of condensation points which are sequentially connected and have weight values larger than a preset value are combined into a mass center, the rest condensation points are respectively combined with the mass centers connected with each other into condensation clusters, and the number of the condensation clusters and the number of the condensation points contained in each condensation cluster are calculated.
The number of aggregation points existing at positions adjacent to any one of the aggregation points is the number of adjacent aggregation points existing at six ends of the aggregation point. It can be understood that, for any aggregation point having a weight value greater than a predetermined value, if the weight values of the aggregation points connected to the aggregation point are all less than or equal to the predetermined value, only the aggregation point is set as a centroid, and the number of the aggregation points of the centroid is 1. In addition, it should be noted that, for any aggregation point with a weight value less than or equal to the predetermined value, if the aggregation point has more than two centroids connected to it, the aggregation point and the centroids with the largest number of aggregation points and connected to it are combined into an aggregation group. In addition, it should be noted that, for any aggregation point with a weight value less than or equal to a predetermined value, if the aggregation point does not have a connected centroid, the aggregation point is regarded as a virtual center, and a plurality of virtual centers connected in sequence are combined into an aggregation group.
As an example, as shown in FIG. 9, the reaction chamber model is divided into 6 liquid storage layers, each of which is provided with 5X 5 three-dimensional solid units, wherein the agglutination points b 22 、c 22 、e 22 Has adjacent coagulation points at all six ends, thus, the coagulation point b 22 、b 23 、d 22 The weight value of (2) is set to 6, and the coagulation points b which are sequentially connected and have a weight value of more than 5 are set 22 、b 23 Merge into centroid A 1 The agglutination point e with a weight of more than 5 22 Is set as the center of mass A 2 Will be in contact with the centroid A 1 Linked agglutination sites a 22 、a 23 、b 12 、b 21 、b 23 、b 32 、c 12 、c 21 、c 23 、c 32 、d 22 And a center of mass A 1 Are combined into a congealed group A [1] Will be in contact with the centroid A 2 Linked agglutination sites e 12 、e 21 、e 23 、e 32 、f 22 And centroid A 2 Are combined into a condensation group A [2] Furthermore, in view of the point of aggregation f 51 、f 52 、f 53 All the weight values of (a) are less than 5, and the agglutination point f 51 、f 52 、f 53 There is no centroid connected to it, so that the coagulation points f are respectively located 51 、f 52 、f 53 Treating as a deficient heart, and treating thisThree virtual centers are combined into a congealing group A [3] Finally, calculating the number of the agglutination groups and the number of the agglutination points contained in each agglutination group to obtain that the number of the agglutination groups of the reaction chamber model is 3, and obtaining an agglutination group A [1] The number of the included aggregation points is 12, and the aggregation group A [2] Containing 6 number of agglutinating points, agglutinating group A [3] The number of the containing aggregation points was 3.
According to the principle, if the number of agglutination groups of the mixed solution is large, the number of agglutination points contained in each agglutination group is also large, so that the blood type antibody concentration of the sample is proved to be large; on the contrary, if the number of the agglutination groups of the mixed solution is small, the number of the agglutination points contained in each agglutination group is also small, so that the blood type antibody concentration of the sample is proved to be small.
It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.
Claims (10)
1. A method for interpreting agglutination conditions, the method comprising:
establishing a reaction cavity model of a containing part according to a proportion, wherein the containing part is of a transparent cylindrical structure, and a mixed solution of a sample and a reagent is stored in a reaction cavity of the containing part;
uniformly dividing the reaction cavity model into a plurality of liquid storage layers along the vertical direction, and carrying out rasterization treatment on each liquid storage layer along the horizontal direction to obtain a plurality of three-dimensional units;
the method comprises the following steps that parallel light generated by a light source is utilized to irradiate a containing part along the horizontal direction, the liquid storage layer at the topmost layer is taken as a starting point, the thickness of the parallel light is extended downwards, the thickness of the parallel light sequentially spans each liquid storage layer, the light path of the parallel light is perpendicular to one end, close to the light source, of a three-dimensional unit, and the width of the parallel light always spans the projection of each liquid storage layer on the width of the parallel light;
in the process that each layer of liquid storage layer is spanned by the thickness of parallel light, a camera is used for collecting two-dimensional overhead images of a plurality of frames of receiving pieces, the part of the two-dimensional overhead images, which is aligned with the reaction cavity, is extracted and is subjected to rasterization processing, and a plurality of two-dimensional visual units are obtained;
acquiring a brightness detection value of each two-dimensional visual unit;
analyzing the brightness detection value of each two-dimensional visual unit by taking a frame as a unit so as to judge whether the mixed liquid is agglutinated or not;
and if the mixed liquor is judged to be agglutinated, sequentially determining the brightness value data of each three-dimensional unit layer by taking the layer as a unit, respectively determining each three-dimensional unit as an agglutinating point or a dispersing point according to the brightness value data of the three-dimensional unit, and judging the agglutinating state of the mixed liquor according to the number and the position of the determined agglutinating points.
2. The agglutination status interpretation method according to claim 1, wherein the specifications of the rasterization process of each layer of liquid storage layer are in accordance with the specifications of the rasterization process of each frame of two-dimensional top view image.
3. The agglutination state interpretation method according to claim 1, wherein the analyzing the brightness detection value of each two-dimensional visual unit in units of frames comprises:
the average brightness value of the two-dimensional overhead image of each frame is obtained by the following steps:
in the formula:
n is the number of two-dimensional visual units of the jth frame of the two-dimensional overhead view image;
4. The agglutination status interpretation method according to claim 3, wherein the luminance detection value of each two-dimensional visual unit is analyzed in units of frames, further comprising:
respectively obtaining the difference value between the brightness detection value of each two-dimensional visual unit and the brightness average value of the two-dimensional overhead image to which the two-dimensional visual unit belongs;
if the absolute value of each difference is smaller than the set threshold, judging that the mixed solution is not agglutinated;
if the absolute value of the difference is larger than the set threshold, the mixed liquid is judged to be agglutinated.
5. The agglutination state interpretation method according to claim 1, wherein said brightness value data comprises a brightness measurement value, a brightness calibration value and a brightness actual value.
6. An agglutination status interpretation method according to claim 5, wherein the brightness measurement value of each three-dimensional stereo unit is in accordance with the following formula:
in the formula:
m is the number of two-dimensional overlook images from the t-th liquid storage layer;
and the ith three-dimensional unit of the tth liquid storage layer and the ith two-dimensional visual unit of each frame of two-dimensional overhead image from the tth liquid storage layer both accord with a position mapping relation.
7. The agglutination state interpretation method according to claim 5, wherein the calibration value of the brightness of any three-dimensional stereo unit of any liquid storage layer except the topmost liquid storage layer conforms to the following formula:
in the formula:
wherein, the (t-1) th layer of liquid storage layer is the upper layer adjacent to the t-th layer of liquid storage layer, and the ith three-dimensional unit of the t-th layer of liquid storage layer is aligned with the ith three-dimensional unit of the (t-1) th layer of liquid storage layer;
and the brightness calibration value of each three-dimensional stereo unit of the topmost liquid storage layer is equal to the respective brightness measurement value.
8. The agglutination status reading method according to claim 5, wherein the brightness of any three-dimensional stereo unit of any liquid storage layer is actually the same as that of the topmost liquid storage layerThe values conform to the following formula:
in the formula:
x is the number of the condensation points which are positioned above the ith three-dimensional unit of the t-th liquid storage layer and conform to the position mapping relation with the three-dimensional unit;
y is the number of dispersed points which are positioned above the ith three-dimensional unit of the t-th layer of liquid storage layer and accord with the position mapping relationship with the three-dimensional unit;
and the actual brightness value of each three-dimensional unit of the topmost liquid storage layer is equal to the respective brightness calibration value.
9. The method according to claim 5, wherein a two-dimensional coordinate system is established based on the positions of the three-dimensional solid cells on the optical path and the actual values of the brightness of the three-dimensional solid cells, and wherein an attenuation line of the scattered light intensity at the condensation point and an attenuation line of the scattered light intensity at the dispersion point are established in the two-dimensional coordinate system, and the three-dimensional solid cells for which the actual values of the brightness have been determined are substituted into the two-dimensional coordinate system according to the positions of the three-dimensional solid cells on the optical path;
confirming a three-dimensional unit positioned outside the scattered light intensity attenuation line of the condensation point as a condensation point;
three-dimensional stereo cells located outside the scattered light intensity attenuation line of the dispersed point were confirmed as dispersed points.
10. The method according to claim 5, wherein the step of determining the agglutination state of the mixed solution comprises:
according to the number of the coagulation points existing at the adjacent positions of the coagulation points, respectively endowing the coagulation points with weight values, combining a plurality of the coagulation points which are sequentially connected and have the weight values larger than a preset value into a mass center, combining the rest coagulation points with the mass centers respectively connected into coagulation groups, and calculating the number of the coagulation groups and the number of the coagulation points contained in each coagulation group.
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