CN115754324A - Micropore plate sampling equipment - Google Patents

Abstract

The invention discloses a micropore plate sample adding device which comprises a base plate, a micropore plate connecting frame, a micropore plate and a sample adding indicating plate, wherein a containing cavity is arranged in the base plate, the micropore plate connecting frame is arranged in the containing cavity, the micropore plate is arranged in the micropore plate connecting frame, the sample adding indicating plate is positioned right above the micropore plate, liquid transferring gun through holes which are the same as the number of micropores on the micropore plate and correspond to the positions of the micropores on the micropore plate are arranged on the sample adding indicating plate, a magnetic part is movably arranged at the top end inside the liquid transferring gun through holes, part of the magnetic part extends to the outside of the liquid transferring gun through holes, through the arranged sample adding indicating plate, when a sample is added into the micropores on the micropore plate through the sample adding indicating plate from the top, a gun body of the liquid transferring gun presses the magnetic part downwards, so that the magnetic part is contained in the through holes of the liquid transferring gun, the magnetic part in the through holes can not be seen from the outside of the sample adding indicating plate to protrude, and an operator can conveniently judge whether the sample is added on the micropore plate corresponding to play a role of auxiliary mark.

Description

Micropore plate sampling equipment

Technical Field

The invention relates to the technical field of medical inspection, in particular to a micropore plate sample adding device.

Background

In biological analysis and detection experiments, microplates are often used as containers for holding detected substances and reactants, and the microplates have different volumes and are 6-well plates, 12-well plates, 48-well plates, 96-well plates, 384-well plates and 1536-well plates, wherein the 96-well, 384-well and 1536-well plates are particularly suitable for high-throughput analysis and detection, such as the most basic enzyme-linked immunosorbent assay (ELI SA) and the most widely used Polymerase Chain Reaction (PCR) in immunology and molecular biology, and the microplates are required to be used as supporting containers for reaction. Reactants in the microplate, including various buffers, samples to be tested, etc., need to be added to the plate wells using a pipettor.

Since the sample loaded into the well is typically a colorless and transparent liquid, it is not easy to distinguish between wells that have already been loaded and wells that have not yet been loaded. For single sample application or when the sample application volume is large, the liquid in the plate hole can be observed from the side to distinguish the sample application; however, in the case where multiple samples need to be loaded into the same well, especially when the samples are loaded in small amounts, which is not enough to be judged by the volume difference, it is impossible to distinguish whether the samples are loaded in the wells by visual observation. To this end, the microplate is indicated by letters and numbers for each plate well (e.g., B07, which represents the plate well in row 2 and column 7 of the microplate) to facilitate identification by the operator during loading. This identification method requires the operator to distinguish whether the sample application is completed in each plate hole by memory, and the operation requires high concentration, and if the operation is disturbed or the continuous sample application process is interrupted, the target plate hole is likely to be missed or even mistakenly found; and the micropore arrangement on the board is comparatively inseparable, and does not have obvious sign between the adjacent hole, therefore the condition of mistake application often appears in the in-process of manual application of sample or big application of sample in batches, to the operation of the different single-hole sample of application of sample volume, the phenomenon that can't find the target pore appears more easily to lead to the experiment failure or the wrong experimental result appears.

Through retrieval, chinese patent application number 201811071755.5 discloses an immunoassay microplate sample adding auxiliary device, and relates to the technical field of medical examination. The sample adding auxiliary device of the immunoassay microplate comprises a supporting frame, a base and a microplate connecting seat, wherein the upper end of the supporting frame is provided with the microplate connecting seat capable of stably placing the microplate, a square grid plate with square grid grooves and the specification of the square grid plate is consistent with that of the micropores of the microplate, and the supporting frame is arranged on the base; and a control system of the sample adding auxiliary device of the microporous plate is designed. On arranging the micro-plate in auxiliary device during this application operation, can clearly accurate record and the micropore of sign application of sample to avoid the testing result deviation that the application of sample mistake leads to, can effectively improve application of sample efficiency, but the device structure is too complicated, and adopted a large amount of optical elements, not only manufacturing cost is too high, be unfavorable for promoting, too much optical elements breaks down easily moreover, cause the device the rate of utilization not high, maintenance and maintenance cost rise.

Through retrieval, chinese patent application No. 202011612337.X discloses an auxiliary sample adding device of an immunoassay microplate, which mainly comprises: the sample adding device comprises a base plate, a frame body, a sample adding plate and a clamping mechanism, wherein the bottom of the frame body is fixedly connected with the base plate, the sample adding plate is connected with the frame body in a sliding mode and is arranged right above the base plate, and the holding mechanism is arranged at the bottom of the base plate. Operate through adding the appearance hole during the application sample, the application appearance hole after the application sample is the state of stretching out, and the operating personnel of being convenient for judges whether the sample has been added to its micropore board that corresponds, but accomplishes the back at the application sample, needs operating personnel to restore to the throne the telescope tube in the application sample hole one by one, need consume longer time, reduces application sample inspection efficiency.

Disclosure of Invention

1. Technical problem to be solved by the invention

Aiming at the defects of the prior art, the invention designs the microporous plate sample adding equipment, through designing the sample adding indicating plate, when a liquid transfer gun adds a sample into micropores on a microporous plate from the upper part through the sample adding indicating plate, the gun body of the liquid transfer gun presses a magnetic part downwards, so that the magnetic part is accommodated in a through hole of the liquid transfer gun, the magnetic part in the through hole of the liquid transfer gun cannot be protruded outside the sample adding indicating plate, an operator can conveniently judge whether the sample is added to the micropore on the microporous plate corresponding to the through hole of the liquid transfer gun, and the function of auxiliary marking is achieved.

2. Technical scheme

In order to solve the above technical problem, the present invention provides a microplate sampling device, comprising:

the bottom plate is internally provided with an accommodating cavity;

a microplate engagement frame mounted within the containment cavity;

the micro-porous plate is arranged in the micro-porous plate connecting frame;

the application of sample indicator board, the application of sample indicator board is located the micropore board directly over, be provided with on the application of sample indicator board with the same and position of micropore volume corresponds's pipetting gun via hole on the micropore board, the inside top activity of pipetting gun via hole is provided with magnetic part, the magnetic part extends to pipetting gun via hole outside, the inside bottom mounting in pipetting gun via hole has the electro-magnet, be provided with the elastic component between magnetic part and the electro-magnet, elastic component one end is fixed with the magnetic part, the elastic component other end is fixed with the electro-magnet, magnetic part and electro-magnet are loop configuration, magnetic part center department is equipped with first through-hole, electro-magnet center department is equipped with the second through-hole, first through-hole and second through-hole diameter are the same and the centre of a circle is located the same axis.

In a preferred embodiment of the present invention, in a natural state of the elastic member, an attractive force of a magnetic attractive force generated by energizing the electromagnet to the magnetic member is smaller than an elastic force of the elastic member.

As a preferable scheme of the invention, supporting rods are vertically arranged at four corners of the top of the bottom plate, sliding sleeves are arranged at four corners of the sample adding indicating plate, and the sample adding indicating plate is sleeved on the supporting rods in a sliding manner through the sliding sleeves.

As a preferable scheme of the invention, a limiting plate is fixedly arranged on the supporting rod close to the middle position, and the limiting plate is used for limiting the position of the sliding sleeve.

As a preferable scheme of the invention, the inner wall of the liquid-transferring gun through hole is inwards recessed to form a limiting groove, the outer side of the bottom end of the magnetic part is provided with a baffle plate, and the baffle plate is arranged in the limiting groove.

As a preferable scheme of the invention, the bottom plate is also provided with a limiting mechanism, the limiting mechanism comprises transverse plates and longitudinal plates which are arranged at the top end of the bottom plate in a transverse and longitudinal staggered manner, and fixing assemblies are arranged between the two ends of the transverse plates and the longitudinal plates and the bottom plate.

As a preferable scheme of the present invention, the outer walls of both sides of the bottom plate along the width direction are provided with first sliding grooves, and the inner sides of both ends of the transverse plate are provided with first sliding blocks slidably connected with the first sliding grooves.

As a preferable scheme of the present invention, the outer walls of both sides of the bottom plate along the length direction are provided with second sliding grooves, and the inner sides of both ends of the longitudinal plate are provided with second sliding blocks slidably connected with the second sliding grooves.

As a preferable scheme of the invention, the fixing component comprises a threaded rod, a knob and a pressing plate, the threaded rod is in threaded connection with two sides of the transverse plate and the longitudinal plate, the top end of the threaded rod is fixedly connected with the knob, and the bottom end of the threaded rod is fixedly connected with the pressing plate.

3. Advantageous effects

Compared with the prior art, the invention has the beneficial effects that:

(1) In the embodiment of the invention, through the arranged sample adding indicating plate, when the microporous plate is subjected to sample adding operation, a pipetting gun adds a sample into micropores on the microporous plate from the upper part through the sample adding indicating plate, when the sample is added, the gun head of the pipetting gun penetrates through the magnetic part and the electromagnet in the through hole of the pipetting gun, the gun body of the pipetting gun presses the magnetic part downwards, the electromagnet is in a powered-on state at the moment, the distance between the magnetic part and the electromagnet is reduced, the attraction of the electromagnet on the magnetic part is increased, the elasticity of the elastic part is overcome, the magnetic part is accommodated in the through hole of the pipetting gun, and the protrusion of the magnetic part in the through hole of the pipetting gun cannot be seen from the outside of the sample adding indicating plate, so that an operator can conveniently judge whether the sample is added to the micropore on the microporous plate corresponding to the through hole of the pipetting gun, the function of auxiliary marking is realized, the situation of repeated adding cannot be caused, the detection or experimental data are more accurate, and the operation is more convenient.

(2) In the embodiment of the invention, through the arranged elastic piece, after the sample adding of the microporous plate is finished, the magnetic piece needs to be reset, at the moment, the electromagnet is powered off, the magnetic attraction of the electromagnet disappears, and the elastic action of the elastic piece can push the magnetic piece to be quickly reset, so that the sample adding of the next microporous plate is convenient to use.

(3) In the embodiment of the invention, the transverse plate and the longitudinal plate can adjust the space size for fixing the micro-porous plates of other specifications on the bottom plate through the arranged limiting mechanism, when the micro-porous plates of other smaller specifications are selected, the micro-porous plate connecting frames of corresponding sizes are arranged, the positions of two ends of the micro-porous plates are limited through the transverse plate and the longitudinal plate, and the other two ends are attached to the inner wall of the accommodating cavity, so that the micro-porous plates of other specifications can be ensured to be in one-to-one correspondence with the positions of the liquid-transfering gun through holes on the sample-adding indicating plate, and the sample-adding indicating plate still plays a role of auxiliary marking.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic view of a split structure according to the present invention;

FIG. 3 is a schematic structural view of a portion of a sample addition indicating plate according to the present invention;

FIG. 4 is a schematic structural diagram of another embodiment of the base plate of the present invention;

fig. 5 is a schematic structural diagram of a fixing assembly according to another embodiment of the invention.

In the figure: 1. a base plate; 101. an accommodating chamber; 102. a first chute; 103. a second chute; 2. the micropore plate connecting frame; 3. a microporous plate; 4. a support bar; 401. a limiting plate; 5. a sample adding indicator board; 501. a sliding sleeve; 502. a pipette is passed through a hole; 5021. a limiting groove; 503. a magnetic member; 5031. a first through hole; 504. An electromagnet; 5041. a second through hole; 505. an elastic member; 506. a baffle plate; 6. a limiting mechanism; 601. A transverse plate; 6011. a first slider; 602. a longitudinal plate; 6021. a second slider; 603. a fixing component; 6031. A threaded rod; 6032. a knob; 6033. and pressing the plate.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.

To facilitate an understanding of the invention, the invention will now be described more fully hereinafter with reference to the accompanying drawings. Several embodiments of the invention are presented in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

Example 1:

referring to fig. 1-3, an embodiment of the invention provides a microplate sample loading device, including a base plate 1, a microplate adapter frame 2, a microplate 3, and a sample loading indicator plate 5, wherein the base plate 1 is internally provided with a receiving cavity 101, the microplate adapter frame 2 is installed in the receiving cavity 101, the microplate 3 is installed in the microplate adapter frame 2, the sample loading indicator plate 5 is located right above the microplate 3, the sample loading indicator plate 5 is provided with pipette via holes 502 having the same number and corresponding positions as the number of the micropores on the microplate 3, the top end of the interior of the pipette via hole 502 is movably provided with a magnetic member 503, a portion of the magnetic member 503 extends to the exterior of the pipette via hole 502, the bottom end of the interior of the pipette via hole 502 is fixed with an electromagnet 504, an elastic member 505 is arranged between the magnetic member 503 and the electromagnet 504, one end of the elastic member 505 is fixed with the magnetic member 503, the other end of the elastic member 505 is fixed with the electromagnet 504, the magnetic member 503 and the electromagnet 504 are both in an annular structure, the center of the magnetic member 503 is provided with a first through hole 5031, the center of the electromagnet 504 is provided with a second through hole 5041, and the first through hole 5031 is located on the same axis as the second through hole 5041.

In a natural state of the elastic member 505, the attraction force of the magnetic attraction force generated by the electromagnet 504 after being energized to the magnetic member 503 is smaller than the elastic force of the elastic member 505.

Specifically, when the sample adding operation is performed on the microporous plate, a pipette adds a sample into the micropores on the microporous plate 3 from the top through the sample adding indicating plate 5, when the sample is added, the tip of the pipette passes through the magnetic part 503 and the electromagnet 504 in the pipette via hole 502, the body of the pipette presses the magnetic part 503 downwards, at this time, the electromagnet 504 is in a powered-on state, the distance between the magnetic part 503 and the electromagnet 504 is reduced, the attraction of the electromagnet 504 to the magnetic part 503 is increased, the elasticity of the elastic part 505 is overcome, the magnetic part 503 is accommodated in the pipette via hole 502, the magnetic part 503 in the pipette via hole 502 cannot be seen from the outside of the sample adding indicating plate 5 to protrude, an operator can conveniently judge whether the sample is added to the micropore on the microporous plate 3 corresponding to the pipette via hole 502, the function of an auxiliary mark is achieved, the situation of repeated adding cannot be caused, the detection or experimental data are more accurate, and the operation is more convenient.

It should be noted that the elastic member 505 is made of a non-magnetic material, and the elastic member 505 may be, but is not limited to, a copper spring, a rubber spring, etc., and does not affect the magnetic attraction of the electromagnet 504, and after the loading of the microplate is completed, the magnetic member 503 needs to be reset, at this time, by turning off the electromagnet 504, the magnetic attraction of the electromagnet 504 disappears, and the elastic member 505 can push the magnetic member 503 to be reset quickly, so as to facilitate the loading and using of the next microplate 3.

Secondly, this micropore board application of sample equipment still includes the controller, and the electro-magnet 504 in every pipette via hole 502 all is connected with controller electrical connection, and this electrical connection mode is prior art, does not do this and does not describe here for a long time.

As an embodiment, please refer to fig. 1, four equal vertical bracing pieces 4 that are provided with in corner at 1 top of bottom plate, sliding sleeve 501 is all installed in four corners of application of sample indicating plate 5, application of sample indicating plate 5 slides through sliding sleeve 501 and cup joints on bracing piece 4, bracing piece 4 through setting up is used for playing the supporting role to application of sample indicating plate 5, application of sample indicating plate 5 slides through sliding sleeve 501 and cup joints in bracing piece 4, application of sample indicating plate 5's installation of being convenient for, after current 3 applications of micropore board are accomplished, through taking off application of sample indicating plate 5, be convenient for change micropore board 3.

Further, be close to intermediate position fixed mounting on the bracing piece 4 and have limiting plate 401, limiting plate 401 is used for restricting the position of sliding sleeve 501, and limiting plate 401 through the setting limits the height position of application of sample indicator board 5 for application of sample indicator board 5 is in reasonable position, can play the effect of supplementary mark with the body of a gun contact of pipetting gun.

As an embodiment, referring to fig. 3, a limit groove 5021 is formed by the inner wall of the pipette via hole 502 being recessed inwards, a baffle 506 is disposed outside the bottom end of the magnetic member 503, the baffle 506 is disposed in the limit groove 5021, the baffle 506 and the limit groove 5021 are configured to limit the range of motion of the magnetic member 503, and the magnetic member 503 is prevented from being disengaged from the pipette via hole 502.

Compared with the prior art, the microplate sample adding device provided by the embodiment of the invention has the advantages that the sample adding indicating plate 5 is sleeved on the supporting rod 4, the sample adding indicating plate 5 is installed, so that the pipette through holes 502 on the sample adding indicating plate 5 correspond to the micropores on the microplate 3 one by one, then a sample is added into the micropores on the microplate 3 through the sample adding indicating plate 5 from the top by the pipette, when the sample is added, the tip of the pipette passes through the magnetic part 503 and the electromagnet 504 in the pipette through hole 502, the body of the pipette pushes the magnetic part 503 downwards, at this time, the electromagnet 504 is in a power-on state, the distance between the magnetic part 503 and the electromagnet 504 is reduced, the attraction of the electromagnet 504 to the magnetic part 503 is increased, the elasticity of the elastic part 505 is overcome, the magnetic part 503 is accommodated in the pipette through hole 502, the magnetic part 503 in the pipette through hole 502 cannot be seen from the outside of the sample adding indicating plate 5, the magnetic part 503 in the pipette through the attraction of the microplate through hole 502, the attraction of the magnetic part 503 can not be added, the attraction of the microplate 3 corresponding to be detected, and the attraction of the electromagnet can be more convenient and more convenient for the operator to use, and more convenient for the detection of the electromagnet for resetting of the electromagnet under the attraction of the magnetic part 503.

Example 2:

the positioning device is basically the same as that in the embodiment 1, and is different in that a limiting mechanism 6 is further disposed on the bottom plate 1, please refer to fig. 4 and 5, the limiting mechanism 6 includes transverse plates 601 and longitudinal plates 602 disposed at the top end of the bottom plate 1 in a transversely and longitudinally staggered manner, the transverse plates 601 and the longitudinal plates 602 can adjust the space size for fixing the micro plates 3 of other specifications on the bottom plate 1, when selecting the micro plates 3 of other smaller specifications, micro plate connecting frames 2 of corresponding sizes are provided, the transverse plates 601 and the longitudinal plates 602 are used for limiting the positions of the two ends of the micro plates 3, and the other two ends are still attached to the inner wall of the accommodating cavity 101, so that the micro plates 3 of other specifications to be replaced can be ensured to be in one-to-one correspondence with the positions of the pipette through holes 502 of the pipette gun on the sample adding indicating plate 5, and the sample adding indicating plate 5 still plays a role of auxiliary marking.

The outer walls of two sides of the bottom plate 1 in the width direction are provided with first sliding grooves 102, the inner sides of two ends of the transverse plate 601 are provided with first sliding blocks 6011 which are slidably connected with the first sliding grooves 102, the stability of the transverse plate 601 in moving adjustment is improved, the outer walls of two sides of the bottom plate 1 in the length direction are provided with second sliding grooves 103, the inner sides of two ends of the vertical plate 602 are provided with second sliding blocks 6021 which are slidably connected with the second sliding grooves 103, and the stability of moving adjustment of the vertical plate 602 is improved.

In addition, a fixing component 603 is arranged between the two ends of the transverse plate 601 and the longitudinal plate 602 and the bottom plate 1, the fixing component 603 includes a threaded rod 6031, a knob 6032 and a pressing plate 6033, the threaded rod 6031 is in threaded connection with the two sides of the transverse plate 601 and the longitudinal plate 602, the top end of the threaded rod 6031 is fixedly connected with the knob 6032, the bottom end of the threaded rod 6031 is fixedly connected with the pressing plate 6033, the bottoms of the transverse plate 601 and the longitudinal plate 602 are both provided with accommodating cavities for accommodating the pressing plate 6033, and after the transverse plate 601 and the longitudinal plate 602 are adjusted to a proper fixing position of the microporous plate 3 of the current specification, an operator rotates the knob 6032 to drive the threaded rod 6031 to rotate, so that the pressing plate 6033 moves downwards and presses against the bottom plate 1, the transverse plate 601 and the longitudinal plate 602 are fixed in position, thereby avoiding sample adding operation of the microporous plate 3 and position deviation of the microporous plate 3, and affecting the sample adding effect.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (9)

1. A microplate sample application device, comprising:

the device comprises a bottom plate (1), wherein an accommodating cavity (101) is formed in the bottom plate (1);

the micro-porous plate connecting frame (2) is arranged in the accommodating cavity (101);

the micro-porous plate (3), the said micro-porous plate (3) is mounted in the micro-porous plate links up the frame (2);

sample application indicator plate (5), sample application indicator plate (5) are located micropore board (3) directly over, be provided with on sample application indicator plate (5) with the same and position of micropore quantity corresponds pipetting gun via hole (502) on micropore board (3), the inside top activity of pipetting gun via hole (502) is provided with magnetic part (503), magnetic part (503) part extends to pipetting gun via hole (502) outside, the inside bottom mounting of pipetting gun via hole (502) has electro-magnet (504), be provided with elastic part (505) between magnetic part (503) and electro-magnet (504), elastic part (505) one end is fixed with magnetic part (503), the elastic part (505) other end is fixed with electro-magnet (504), magnetic part (503) is the loop configuration with electro-magnet (504), magnetic part (503) center department is equipped with first through-hole (5031), electro-magnet (504) center department is equipped with second through-hole (50341), first through-hole (5031) and second through-hole (5041) diameter are the same and are located the same axis centre of a circle 5041.

2. The microplate sample application device according to claim 1, wherein in a natural state of the elastic member (505), an attractive force of a magnetic attractive force generated after the electromagnet (504) is energized to the magnetic member (503) is smaller than an elastic force of the elastic member (505).

3. The microplate sample adding device according to claim 1, wherein support rods (4) are vertically arranged at four corners of the top of the bottom plate (1), sliding sleeves (501) are arranged at four corners of the sample adding indicating plate (5), and the sample adding indicating plate (5) is slidably sleeved on the support rods (4) through the sliding sleeves (501).

4. The microplate sample application device according to claim 3, wherein a position limiting plate (401) is fixedly mounted on the support rod (4) near the middle position, and the position limiting plate (401) is used for limiting the position of the sliding sleeve (501).

5. The microplate sample adding device according to claim 1, wherein the inner wall of the pipette gun via hole (502) is recessed inwards to form a limiting groove (5021), a baffle (506) is arranged on the outer side of the bottom end of the magnetic member (503), and the baffle (506) is placed in the limiting groove (5021).

6. The microplate sample adding device according to claim 1, wherein the base plate (1) is further provided with a limiting mechanism (6), the limiting mechanism (6) comprises a transverse plate (601) and a longitudinal plate (602) which are arranged in a transversely-longitudinally staggered manner at the top end of the base plate (1), and fixing components (603) are arranged between the two ends of the transverse plate (601) and the longitudinal plate (602) and the base plate (1).

7. The microplate sample adding device according to claim 6, wherein the outer walls of two sides of the bottom plate (1) in the width direction are provided with first sliding grooves (102), and the inner sides of two ends of the transverse plate (601) are provided with first sliding blocks (6011) slidably connected with the first sliding grooves (102).

8. The microplate sampling device as claimed in claim 6, wherein the outer walls of two sides of the bottom plate (1) along the length direction are provided with second sliding grooves (103), and the inner sides of two ends of the longitudinal plate (602) are provided with second sliding blocks (6021) slidably connected with the second sliding grooves (103).

9. The microplate sample adding device according to claim 6, wherein the fixing assembly (603) comprises a threaded rod (6031), a knob (6032) and a pressing plate (6033), the threaded rod (6031) is in threaded connection with two sides of the transverse plate (601) and the longitudinal plate (602), the top end of the threaded rod (6031) is fixedly connected with the knob (6032), and the bottom end of the threaded rod (6031) is fixedly connected with the pressing plate (6033).

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