CN115743912B

CN115743912B - Kit of re-soluble reagent

Info

Publication number: CN115743912B
Application number: CN202211544963.9A
Authority: CN
Inventors: 李保; 林宝宝; 郭永琪; 杨毅
Original assignee: Beijing Zijing Biotechnology Co ltd
Current assignee: Beijing Zijing Biotechnology Co ltd
Priority date: 2022-12-04
Filing date: 2022-12-04
Publication date: 2026-01-23
Anticipated expiration: 2042-12-04
Also published as: CN115743912A

Abstract

This invention provides a reagent kit for resolvable reagents, comprising a kit body having a receiving through-hole. A sealing element is detachably connected to a first end of the receiving through-hole, and a piston assembly is connectable to a second end of the receiving through-hole. A first reagent is placed in the space between the piston assembly and the sealing element. The piston assembly includes a piston, and a receiving element is connected to a stopper column within the receiving through-hole. The receiving element contains a second reagent. The kit also includes a push-down device capable of detaching the receiving element from the stopper column. After the receiving element detaches from the stopper column, the second reagent in the receiving element can be resolvated with the first reagent. This invention allows for the separate and independent sealing of the two reagents, facilitating storage, production, and transportation. Furthermore, the push-down device detaches the receiving element from the stopper column to resolvate the two reagents, eliminating the risk of reagent transfer to the external environment and ensuring the accuracy of the test results.

Description

Kit of re-soluble reagent

Technical Field

The invention belongs to the field of preparation kit design, and particularly relates to a kit for a re-soluble reagent.

Background

The biological preparation can well maintain the biological activity in the freeze-dried state, so the freeze-dried product of the reagent is widely applied. However, in the application process of the freeze-dried reagent, the freeze-dried reagent needs to be re-dissolved, namely, the freeze-dried reagent is packaged in a container, then a re-solution is added, and then the re-dissolved freeze-dried reagent is taken out and applied by a pipette. Therefore, designing a reagent kit of a re-dissolvable reagent which is convenient for re-dissolution operation, pollution risk in the operation process and easy to store, produce and transport is also an urgent requirement.

Disclosure of Invention

Therefore, the technical problem to be solved by the invention is to provide a reagent kit capable of redissolving the reagent, so as to overcome the defects that the accuracy of a detection result is reduced and the operation flow is complicated due to pollution caused by redissolving the freeze-dried reagent in a pipetting mode in the prior art.

In order to solve the problems, the invention provides a reagent kit capable of redissolving a reagent, which comprises a kit body, wherein the kit body is provided with a containing through hole, a plugging piece is detachably connected to a first end of the containing through hole, a piston assembly can be connected to a second end of the containing through hole, a first reagent is contained in a space between the piston assembly and the plugging piece, the piston assembly comprises a piston, a containing piece is connected to a plug of the piston in the containing through hole, a second reagent is contained in the containing piece, and the kit further comprises a pushing device, the pushing device can enable the containing piece to fall off from the plug, and after the containing piece is separated from the plug, the second reagent in the containing piece can be redissolved with the first reagent.

In some embodiments, the accommodating part comprises a first gasket and a second gasket, the first gasket and the second gasket are assembled on the plug at intervals along the axial direction of the piston, the first gasket is positioned at one side of the second gasket away from the first reagent, and the second reagent is accommodated in a gap between the first gasket and the second gasket.

In some embodiments, the piston is a plug, a blind bore is configured on an end of the plug facing the first reagent, and the first gasket and the second gasket are assembled with interference within the blind bore.

In some embodiments, the pushing down device comprises a lancet having a push-off position that pierces the plunger and disengages the receptacle from the plunger, the lancet being in the push-off position when reconstitution of the first and second reagents is desired.

In some embodiments, the pushing down device further comprises a push plate disposed on the tail of the lancet.

In some embodiments, the lancets are at least two, and at least two of the lancets are evenly spaced around the circumference of the piston.

In some embodiments, the end face of the boosting plate facing the piston is provided with a clearance retaining ring which is removed from the end face of the piston when it is desired to disengage the receiving element from the plug.

In some embodiments, the clearance retaining ring is disposed around the outside of the lancet and is connected to the thrust plate by a virtual cut.

In some embodiments, the first gasket is formed from a hard material.

In some embodiments, the first reagent is a liquid reagent and the second reagent is a dry powder reagent.

According to the reagent kit capable of redissolving the reagent, when the accommodating part is in an initial state, namely the accommodating part is connected to the plunger of the piston, the second reagent and the first reagent are respectively in independent spaces, so that the aim of respectively and independently sealing and storing two reagents to be redissolved is fulfilled, the two reagents are particularly easy to store, produce and transport in the state, when the two reagents are required to be redissolved, the accommodating part is separated from the plunger by the pushing device, the second reagent in the accommodating part is contacted with the first reagent along with the falling process of separating the accommodating part, the redissolving process does not have the transfer of the reagents in the external environment, the risk of pollution of the preparation is completely eradicated, the accuracy of a subsequent detection result is ensured, and the redissolving process is simple and convenient to operate.

Drawings

FIG. 1 is a schematic structural view of a reagent kit for reconstitutable reagents according to an embodiment of the present invention, with the container in an initial state;

FIG. 2 shows a condition in which the container of the reagent-reconstitutable cartridge of FIG. 1 is pushed away from the plunger by the pushing-down device and falls off, in which the second reagent will follow the container to the underlying first reagent;

Fig. 3 is a schematic diagram showing a state of redissolving the first reagent and the second reagent after the container falls to the first reagent.

The reference numerals are expressed as:

1. the kit comprises a kit body, 11 parts of accommodating through holes, 2 parts of blocking parts, 31 parts of pistons, 4 parts of pushing-down devices, 41 parts of puncture needles, 42 parts of boosting plates, 43 parts of clearance retaining rings, 51 parts of first gaskets, 52 parts of second gaskets, 101 parts of first reagents, 102 parts of second reagents.

Detailed Description

Referring to fig. 1 to 3 in combination, according to an embodiment of the present invention, there is provided a reagent kit including a kit body 1, the kit body 1 having a receiving through hole 11, a blocking member 2 detachably connected to a first end of the receiving through hole 11, a piston assembly connected to a second end of the receiving through hole 11, a first reagent 101 disposed in a space between the piston assembly and the blocking member 2, the piston assembly including a piston 31, a second reagent 102 disposed in the receiving through hole 11 and connected to a receiving member (not labeled in the drawing) disposed in the receiving member, and a pushing device 4 capable of releasing the receiving member from the blocking member, wherein the second reagent 102 disposed in the receiving member is capable of being re-dissolved with the first reagent 101 after the receiving member is released from the blocking member.

In this technical scheme, when the holding member is in the initial state, that is, the holding member is connected to the plunger of the piston 31, the second reagent 102 and the first reagent 101 are respectively in independent spaces, so as to achieve the purpose of separately and independently sealing and storing two reagents to be reconstituted, in this state, the holding member is easy to store, produce and transport, when two reagents are required to be reconstituted, the holding member is separated from the plug by the pushing device 4, and the second reagent 102 in the holding member contacts with the first reagent 101 to complete the reconstitution along with the falling process of separating the holding member, the transfer of the reagent in the external environment is avoided in the reconstitution process, the risk of pollution of the preparation is completely eradicated, the accuracy of the subsequent detection result is ensured, the reconstitution flow is simple, and the operation is convenient.

In a specific embodiment, the accommodating member includes a first gasket 51 and a second gasket 52, the first gasket 51 and the second gasket 52 are assembled on the plug at intervals along the axial direction of the piston 31, the first gasket 51 is located on one side of the second gasket 52 away from the first reagent 101, and the second reagent 102 is accommodated in a gap between the first gasket 51 and the second gasket 52, it can be understood that after the first gasket 51 and the second gasket 52 are assembled on the plug, the second reagent 102 should be stored in a sealed manner to ensure that the two reagents do not come into contact when the reagent kit is changed in position, for example, upside down. In a preferred embodiment, the piston 31 is a rubber plug, one end of the plug facing the first reagent 101 is configured with a blind hole cavity, the first gasket 51 and the second gasket 52 are assembled in the blind hole cavity in an interference manner, and by adopting the interference assembly manner of the first gasket 51 and the second gasket 52, the independent sealing storage of the second reagent 102 can be ensured by utilizing the deformability and the tightness of the rubber plug, meanwhile, the structure can easily ensure the separation between the accommodating part and the plug under the action of the pushing-down device 4, and after the accommodating part is separated, as the second reagent 102 is accommodated in the gap position between the first gasket 51 and the second gasket 52, the first reagent 101 and the second reagent 102 are fully contacted and re-dissolved through the gap, so that the contact area is larger, and the re-dissolution efficiency can be improved. It should be noted that, the thicknesses of the first gasket 51 and the second gasket 52 in the accommodating member are relatively small (the thicknesses in the drawing are only examples), and they are at the bottom of the kit body 1 after falling, and do not occupy the formed mixed solvent disadvantageously.

As a preferred embodiment of the pushing down device 4, the pushing down device 4 comprises a lancet 41, wherein the lancet 41 is provided with a piercing piston 31 and is separated from a pushing-off position of the plug, the lancet 41 is positioned at the pushing-off position when the first reagent 101 and the second reagent 102 need to be dissolved again, and the containing piece is separated from the plug when the lancet 41 is positioned at the pushing-off position, so that the purpose of applying force to the containing piece by the pushing down device 4 is achieved.

In some embodiments, the pushing device 4 further includes a pushing plate 42, where the pushing plate 42 is disposed at the tail of the lancet 41, that is, at the end of the lancet 41 away from the piston 31, and in a specific use process, an operator can apply a finger to the pushing plate 42 to make the lancet 41 pierce the piston 31 and further push the accommodating element, and in other application conditions, a corresponding pushing rod can also apply a force to the pushing plate 42 to achieve the purpose of mechanical operation.

Preferably, the number of the lancets 41 is at least two, the at least two lancets 41 are uniformly spaced around the circumference of the piston 31, and the at least two lancets 41 can uniformly apply force to the circumference of the accommodating member, so that the accommodating member can be ensured to be smoothly separated from and fall off.

In a possible embodiment, the pushing down means 4 may be provided independently of the cartridge body 1, and the pushing down means 4 may be taken only when pushing down the receiving member is required, which is obviously cumbersome, and in a preferred embodiment the end surface of the pushing up plate 42 facing the piston 31 is provided with a clearance retaining ring 43, the clearance retaining ring 43 being removed from the end surface of the piston 31 when the receiving member is required to be separated from the plug, in particular the clearance retaining ring 43 may have a collar shape, which is supported between the pushing up plate 42 and the end surface of the cartridge body 1 to prevent the pushing up plate 42 from being moved to the receiving member side in the axial direction of the cartridge body 1, which is obviously achieved when the first reagent 101 and the second reagent 102 are not required to be re-dissolved, and when the two are required to be re-dissolved, the clearance retaining ring 43 is removed to release the restriction of the axial movement of the pushing up plate 42.

In some embodiments, a clearance ring 43 is disposed around the outside of lancet 41 and is connected to thrust plate 42 by a virtual cut that provides a virtual connection between clearance ring 43 and thrust plate 42 using a virtual cut device to allow easy pulling away of the clearance ring 43 when it is desired to remove the constraint.

In some embodiments, first pad 51 is formed of a hard material, such as a hard plastic as described above, e.g., pp, pc, etc., to ensure that it is not pierced by lancet 41.

As a preferred solution, the first reagent 101 is a liquid reagent, and the second reagent 102 is a dry powder reagent, specifically a solid lyophilized reagent, that is, the second reagent 102 is stored in the accommodating part, so that the assembling of the accommodating part and the plug can be facilitated.

It will be readily appreciated by those skilled in the art that the above advantageous ways can be freely combined and superimposed without conflict.

The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the invention. The foregoing is merely a preferred embodiment of the present invention, and it should be noted that it will be apparent to those skilled in the art that modifications and variations can be made without departing from the technical principles of the present invention, and these modifications and variations should also be regarded as the scope of the invention.

Claims

1. A reagent kit for resolvable reagents, characterized in that it comprises a reagent kit body (1), the reagent kit body (1) having a receiving through hole (11), a sealing member (2) being detachably connected to a first end of the receiving through hole (11), a piston assembly being connectable to a second end of the receiving through hole (11), a first reagent (101) being placed in the space between the piston assembly and the sealing member (2), the piston assembly comprising a piston (31), a receiving member being connected to a plug in the receiving through hole (11), the receiving member containing a second reagent (102), and the reagent kit further comprising a pushing device (4), the pushing device (4) being capable of pushing the receiving member... The second reagent (102) in the container can be re-dissolved with the first reagent (101) after the container is detached from the stopper. The container includes a first gasket (51) and a second gasket (52). The first gasket (51) and the second gasket (52) are assembled on the stopper at intervals along the axial direction of the piston (31). The first gasket (51) is located on the side of the second gasket (52) away from the first reagent (101). The second reagent (102) is contained in the gap between the first gasket (51) and the second gasket (52). The first gasket (51) and the second gasket (52) are independent and not connected to each other.

2. The kit according to claim 1, wherein the piston (31) is a rubber stopper, a blind hole cavity is constructed on one end of the stopper facing the first reagent (101), and the first gasket (51) and the second gasket (52) are interference-fitted into the blind hole cavity.

3. The kit according to claim 1, wherein the push-down device (4) includes a piercing needle (41) having a push-away position that pierces the piston (31) and disengages the receiving member from the plug, and the piercing needle (41) is in the push-away position when it is necessary to reconstitute the first reagent (101) and the second reagent (102).

4. The kit according to claim 3, wherein the pushing device (4) further includes a pusher plate (42) disposed at the tail of the puncture needle (41).

5. The kit according to claim 4, wherein the puncture needle (41) is at least two, and the at least two puncture needles (41) are evenly spaced around the piston (31) in the circumferential direction.

6. The kit according to claim 4, wherein the booster plate (42) is provided with a gap retaining ring (43) on the end face facing the piston (31), the gap retaining ring (43) being removed from the end face of the piston (31) when it is necessary to disengage the receiving member from the plunger.

7. The kit according to claim 6, wherein the gap retaining ring (43) is disposed around the outside of the puncture needle (41) and is connected to the push plate (42) through a slit.

8. The kit according to any one of claims 1 to 7, wherein the first pad (51) is formed of a rigid material.

9. The kit according to claim 1, wherein the first reagent (101) is a liquid reagent and the second reagent (102) is a dry powder reagent.