CN115684021B - A microfluidic chip and detection device for turbidimetric detection - Google Patents

Abstract

The invention relates to the technical field of medical devices, and in particular refers to a microfluidic chip for turbidimetric detection, which includes: a base body, a blood collection tube cavity is provided on the base body, and a guide strip is provided on one side of the blood collection tube cavity, A plurality of observation chambers are provided at the bottom of the base body; the plurality of observation chambers are connected to the first channel through multiple channels, the first channel is connected to the second channel, the second channel is connected to the third channel, the third channel is connected to the blood collection tube cavity, and a third channel is opened on the front side of the base body. One air hole, the first air hole is connected to the first air channel, the first air channel is connected to the second air channel, and the second air channel is connected to multiple observation chambers through multiple air channels; the front and rear sides of the base body are respectively bonded to the front side cover and the rear The side cover and the front side cover are provided with a second air hole corresponding to the first air hole, and the first air hole is connected with the second air hole. When performing turbidimetric detection, the present invention only needs to vacuum through the pores to suck the tested sample into multiple observation chambers for detection, and the sample adding process is completed in one step.

Description

A microfluidic chip and detection device for turbidimetric detection

Technical field

The present invention relates to the technical field of medical devices, and in particular, to a microfluidic chip and a detection device used for turbidimetric detection.

Background technique

Microfluidic technology refers to the science and technology involved in systems that use microchannels to process or manipulate tiny fluids. It is an emerging interdisciplinary subject involving chemistry, fluid physics, microelectronics, new materials, biology and biomedical engineering. Microfluidic technology can concentrate the detection process on centimeter to micron-scale chips, miniaturizing and automating the entire detection. It is a promising technical direction in the field of in vitro diagnostics.

Turbidimetry is one of the main detection methods for in vitro diagnostics. The detection principle is that the analyte in the sample reacts specifically with the reagent to form a complex, and the complex polymerizes within a certain period of time to produce turbidity. When light passes through a solution, it can be absorbed by the compound. The greater the amount of the compound, the amount of light absorbed is positively correlated with the amount of the compound within a certain range. Therefore, by optically comparing the depth of color of a solution, the concentration of the analyte specifically bound to the reagent in the sample can be determined.

Chinese invention patent application CN106970026A discloses a biochip box and its operating method. Chinese utility model patent CN207198028U discloses a detection device for a biochemical instrument, and discloses diagnostic equipment and consumables that conventionally adopt turbidimetry. The consumables are cuvettes. , with a single cup or multiple cups. The test sample and the reagent are mixed manually or automatically through equipment and then added to the cuvette. The cuvette is used for turbidimetric detection between the light source and the detector. The cuvette only plays the role of containing the reaction solution and transmitting turbidimetry. Usually the pretreatment and preparation process of the reaction solution is complicated. Manual preparation is labor-intensive and easy to introduce human interference. If the equipment is automatically implemented, the design of the equipment is difficult, and the volume and floor space of the equipment are generally large.

Chinese invention patent application CN108169499A discloses a method for coagulation analysis based on a biochemical reagent disk. Biochemical detection is realized through a disk microfluidic chip. After the tested sample is added to the microfluidic chip, it is diluted with a preset liquid, and then the microfluidic chip Driven and rotated by the device, the diluted sample to be tested is thrown into the reaction chamber at the outer edge of the chip by centrifugal force, and reacts after mixing with the preset freeze-dried reagents in the chamber. During the rotation of the disk, each reaction chamber passes through the light source and detector inside the device for detection. The design of the disc chamber and flow channel in this solution is complex, and high-speed rotation places high demands on the dynamic balance of the disc; the testing equipment needs to drive the disc to rotate at high speed through a motor, which is large in size.

Contents of the invention

In order to solve the technical problems in the prior art that the design of microfluidic chips and detection devices for turbidimetric detection is difficult, and the volume and floor space are large, an embodiment of the present invention provides a microfluidic chip and detection device for turbidimetric detection. Control chip, the microfluidic chip includes: a base body, a blood collection tube cavity is provided on the base body, guide strips are provided on both sides of the base body, and a plurality of observation chambers are provided at the bottom of the base body;

A plurality of the observation chambers are connected to a first channel through a plurality of channels, the first channel is connected to a second channel, the second channel is connected to a third channel, and the third channel is connected to the blood collection tube cavity,

A first air hole is provided on the front side of the base body, the first air hole is connected to a first air channel, the first air channel is connected to a second air channel, and the second air channel is connected to a plurality of the observations through multiple air channels. cavity; cavity

The front and back sides of the base body are respectively bonded to a front side cover and a rear side cover. The front side cover has a second air hole corresponding to the first air hole. When the front side cover is closed on the base body, The first pore is connected with the second pore;

Reagent balls and magnetic beads are respectively preset in the plurality of observation chambers.

In a preferred embodiment, a hollow puncture needle is provided in the lumen of the blood collection tube, and the puncture needle is connected to the third channel.

In a preferred embodiment, the second channel is provided with an infrared detection point for liquid inlet.

In a preferred embodiment, the plurality of observation cavities include a first observation cavity, a second observation cavity, a third observation cavity and a fourth observation cavity;

The first observation cavity is connected to the first channel through a fourth channel, the second observation cavity is connected to the first channel through a fifth channel, and the third observation cavity is connected to the first channel through a sixth channel. , the fourth observation cavity is connected to the first channel through a seventh channel.

In a preferred embodiment, the first observation chamber is connected to the second airway through a third airway, the second observation chamber is connected to the second airway through a fourth airway, and the third observation chamber is connected through The fifth airway is connected to the second airway, and the fourth observation chamber is connected to the second airway through the sixth airway.

In a preferred embodiment, the third airway is provided with a first infrared detection point, the fourth airway is provided with a second infrared detection point, the fifth airway is provided with a third infrared detection point, and the The sixth airway is set with a fourth infrared detection point.

Another embodiment of the present invention provides a detection device for turbidimetric detection. The detection device includes: a card slot front shell and a card slot rear shell. A microfluidic chip is inserted into the card slot front shell and the card slot. Between the groove and the rear shell,

The front shell of the card slot is provided with a pressure plate assembly. A return spring is provided between the front shell of the card slot and the pressure plate assembly. The pressure plate assembly is configured to move up and down along the front shell of the card slot and swing back and forth;

When the microfluidic chip moves downward between the front shell of the card slot and the back shell of the card slot, the microfluidic chip squeezes the pressure plate assembly and moves downward along the front shell of the card slot, and at the same time, the pressure plate The component swings toward the microfluidic chip and snaps onto the front side of the microfluidic chip;

When the microfluidic chip moves upward between the front shell of the card slot and the back shell of the card slot, the return spring pulls the pressure plate assembly to move upward along the front shell of the card slot, and at the same time, the pressure plate assembly moves back Swing toward the microfluidic chip to separate from the front side of the microfluidic chip.

In a preferred embodiment, the front housing of the card slot includes a guide groove, and the guide groove has an inclined slope.

The pressure plate assembly includes an air nozzle, a pressure plate is fixed above the air nozzle, the pressure plate includes a first section structure and a second section structure, the first section structure is fixed to the air nozzle, and the second section structure is fixed A guide post, the guide post is in contact with the guide groove;

A limiting post is fixed below the gas nozzle. When the microfluidic chip moves downward between the front shell of the card slot and the back shell of the card slot, the microfluidic chip squeezes the limiting column and drives the The pressure plate assembly moves downward along the front shell of the slot.

In a preferred embodiment, the air nozzle is provided with a ventilation channel, and a sealing ring is provided on the end face of the air nozzle connected to the ventilation channel;

When the pressure plate assembly is fastened to the front side of the microfluidic chip, the sealing ring abuts against the second air hole of the microfluidic chip.

In a preferred embodiment, the detection device further includes a mixing component, a plurality of light sources and a plurality of detectors, the mixing component includes a progressive motor, and a plurality of permanent magnets located in the detection device;

When the pressure plate assembly is fastened to the front side of the microfluidic chip, multiple light sources are located on the front side of the microfluidic core and correspond to multiple observation chambers of the microfluidic chip, and multiple detectors are located on the microfluidic core. The back side of the microfluidic chip corresponds to the multiple observation chambers of the microfluidic chip;

Multiple permanent magnets are located at the bottom of the microfluidic core and correspond to multiple observation chambers of the microfluidic chip. The progressive motor drives the multiple permanent magnets to reciprocate and stir the reagent balls and magnetic beads in the multiple observation chambers.

The beneficial effects brought by the technical solutions provided by the embodiments of the present invention include at least:

The present invention proposes a microfluidic chip and a detection device for turbidimetric detection. When the microfluidic chip performs turbidimetric detection, it only needs to vacuum through the pores to suck the tested sample into multiple observation chambers. For testing, the sample addition process is completed in one step, greatly reducing the sample addition steps.

The present invention proposes a microfluidic chip and a detection device for turbidimetric detection, which adopt microfluidic technology. Multiple observation chambers inside the microfluidic chip are pre-set with reagent balls, which avoids the preparation of reagents and can be stored at room temperature. transport. Nephelometric measurement can be performed by directly inserting the blood collection tube into the microfluidic chip. There is no need to preprocess the sample and reagents to be tested, and the entire process of driving the sample to be tested, quantification of the reaction solution, mixing of the reagents, and detection is automatically completed. The microfluidic chip is a card-type design, and the liquid inside is driven by pneumatic pressure. The interface and equipment are simple, convenient and fast.

The invention proposes a microfluidic chip and a detection device for turbidimetric detection. Multiple observation chambers inside the microfluidic chip are pre-set with magnetic beads, and cooperate with the reciprocating permanent magnets of the detection device to achieve rapid mixing of reagents.

The present invention proposes a microfluidic chip and a detection device for turbidimetric detection. After the microfluidic chip is inserted into the detection device, it can automatically realize locking and gas path sealing. The structure is stable and reliable. The microfluidic chip and detection device are designed as The platform design is not specific to a certain testing item and is suitable for all testing items using transmission turbidimetric methodology.

Description of the drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can also be obtained based on these drawings without exerting creative efforts.

Figure 1 is an exploded view from the front side of a microfluidic chip used for turbidimetric detection in one embodiment of the present invention.

Figure 2 is an exploded view from the rear side of a microfluidic chip used for turbidimetric detection in one embodiment of the present invention.

FIG. 3 is a schematic diagram of the internal structure of a microfluidic chip used for turbidimetric detection in one embodiment of the present invention from the front side.

Figure 4 is a schematic three-dimensional structural diagram of a detection device for turbidimetry detection from the front side in one embodiment of the present invention.

Figure 5 is a schematic three-dimensional structural diagram of a detection device used for turbidimetric detection in an embodiment of the present invention from a rear side perspective.

Figure 6 is a schematic view from the front side of a detection device for turbidimetric detection in an open state with the pressure plate assembly in one embodiment of the present invention.

Fig. 7 is a cross-sectional view taken along line B-B in Fig. 6 .

Figure 8 is a schematic view of the front side of a detection device for turbidimetric detection in a closed state with the pressure plate assembly in one embodiment of the present invention.

Fig. 9 is a cross-sectional view taken along line A-A in Fig. 8 .

Figure 10 is a schematic diagram of the working state of a detection device used for turbidimetric detection in one embodiment of the present invention.

Figure 11 is a schematic diagram of the working state of a microfluidic chip used for turbidimetric detection in one embodiment of the present invention.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention.

The terms "first", "second", "third", "fourth", etc. (if present) in the description and claims of the present invention and the above-mentioned drawings are used to distinguish similar objects and are not necessarily used to Describe a specific order or sequence. It is to be understood that the figures so used are interchangeable under appropriate circumstances so that the embodiments of the invention described herein, for example, can be practiced in sequences other than those illustrated or described herein. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions, e.g., a process, method, system, product, or apparatus that encompasses a series of steps or units and need not be limited to those explicitly listed. Those steps or elements may instead include other steps or elements not expressly listed or inherent to the process, method, product or apparatus.

As shown in Figure 1, an exploded view from the front side of a microfluidic chip for turbidimetric detection in one embodiment of the present invention is shown. Figure 2 shows an exploded view of a microfluidic chip for turbidimetric detection in one embodiment of the present invention. An exploded view of the microfluidic chip from the rear side. Figure 3 shows a schematic diagram of the internal structure of a microfluidic chip used for turbidimetric detection in one embodiment of the present invention. According to the embodiment of the present invention, it is provided A microfluidic chip used for turbidimetric detection, including: a base body 1, the front and back sides of the base body 1 are respectively bonded with a front side cover 2 and a rear side cover 3.

The base 1 is provided with a blood collection tube cavity 101 for accommodating the blood collection tube. A handle 102 is provided above the base 1 . Guide bars 104 are provided on both sides of the base 1 . The base body 1 is also provided with a first positioning hole 103 and a front side cover positioning pin 124.

The front cover 2 is provided with a front cover positioning hole 201 and a second positioning hole 202. The front cover 2 is bonded to the front side of the base 1, and the front cover positioning pin 124 is fastened to the front cover positioning hole 201. Together. The first positioning hole 103 corresponds to the second positioning hole 202 . The rear side cover 3 is bonded to the rear side of the base body 1 .

According to the embodiment of the present invention, a plurality of observation cavities are provided at the bottom of the base 1 . The plurality of observation chambers are connected to the first channel 107 through multiple channels, the first channel 107 is connected to the second channel 122, the second channel 122 is connected to the third channel 106, and the third channel 106 is connected to the blood collection tube chamber 101. The second channel 122 is provided with a liquid infrared detection point 123. Reagent balls 4 and magnetic beads 5 are respectively preset in multiple observation chambers. The magnetic beads 5 can be attracted by permanent magnets.

A hollow puncture needle 7 is provided in the blood collection tube cavity 101. The hollow channel inside the puncture needle 7 is connected to the third channel 106. When the blood collection tube is inserted into the blood collection tube cavity 101, the puncture needle 7 pierces the sealing film of the blood collection tube and inserts it into the blood collection tube. Sampling is carried out through the hollow channel inside the puncture needle 7 .

In a specific embodiment, the plurality of observation cavities include a first observation cavity 109 , a second observation cavity 110 , a third observation cavity 111 and a fourth observation cavity 112 .

The first observation cavity 109 is connected to the first channel 107 through the fourth channel 113, the second observation cavity 110 is connected to the first channel 107 through the fifth channel 114, the third observation cavity 111 is connected to the first channel 107 through the sixth channel 115, and the fourth The observation cavity 112 communicates with the first channel 107 through the seventh channel 116 .

According to the embodiment of the present invention, a first air hole 105 is provided on the front side of the base body 1. The first air hole 105 is connected to the first air channel 121, the first air channel 121 is connected to the second air channel 108, and the second air channel 108 passes through multiple air channels. Connect multiple observation chambers.

In a specific embodiment, the first observation chamber 109 is connected to the second airway 108 through the third airway 117, the second observation chamber 110 is connected to the second airway 108 through the fourth airway 118, and the third observation chamber 111 is connected through the fifth airway 117. The airway 119 is connected to the second airway 108, and the fourth observation chamber 112 is connected to the second airway 108 through the sixth airway 120.

The filter element 6 is provided at a position where the second airway 108 is connected to multiple airways, that is, where the second airway 108 is connected to the third airway 117 , where the second airway 108 is connected to the fourth airway 118 , and where the second airway 108 is connected to the fourth airway 118 . The position where the fifth air channel 119 is connected and the position where the second air channel 108 is connected to the sixth air channel 120 are both provided with a filter element 6. The filter element 6 can pass gas but not liquid.

The front cover 2 has a second air hole 203 corresponding to the first air hole 105. When the front cover 2 is closed on the base 1, the first air hole 105 and the second air hole 203 are connected correspondingly.

The third airway 117 is set with a first infrared detection point, the fourth airway 118 is set with a second infrared detection point, the fifth airway 119 is set with a third infrared detection point, and the sixth airway 120 is set with a fourth infrared detection point.

In some preferred embodiments, freeze-dried reagent balls 4 are preset in the first observation chamber 109, the second observation chamber 110, the third observation chamber 111 and the fourth observation chamber 112. The reagent balls 4 may not be preset. Contrast.

The liquid infrared detection point 123, the first infrared detection point, the second infrared detection point, the third infrared detection point, and the fourth infrared detection point can detect whether there is liquid flowing through the infrared probe.

In some preferred embodiments, the materials of the base body 1, the front side cover 2, and the rear side cover 3 include but are not limited to PC, ABS, PMMA, and PP.

In some preferred embodiments, the front side cover 2 and the rear side cover 3 are bonded to the base 1 , and the bonding process includes but is not limited to hot pressing, bonding, ultrasonic welding, and laser welding.

In some preferred embodiments, multiple observation chambers can be designed as sample reaction chambers, negative control chambers, positive control chambers, baseline control chambers, blank control chambers, etc. according to requirements.

In some preferred embodiments, the volumes of multiple observation chambers can be determined as fixed volumes according to needs, thereby achieving quantification of the reaction system.

As shown in Figure 4, a schematic structural diagram of a front side view of a detection device for turbidimetric detection in one embodiment of the present invention is shown. Figure 5 shows a detection device for turbidimetric detection in one embodiment of the present invention. Schematic diagram of the rear perspective structure of the device. According to an embodiment of the present invention, a detection device for turbidimetric detection is provided, including: a slot front shell 8, a slot rear shell 9, a mixing assembly 13, and a plurality of The light source 12, a plurality of detectors 14, and the microfluidic chip are inserted between the card slot front shell 8 and the card slot rear shell 9 for turbidimetric detection.

The front housing 8 of the card slot is provided with a pressure plate assembly 10. A return spring 11 is provided between the front housing 8 and the pressure plate assembly 10. The pressure plate assembly 10 is configured to move up and down along the front shell 8 of the card slot and swing back and forth.

As shown in Figure 6, a schematic view of the front side of a detection device for turbidimetric detection in an open state with the pressure plate assembly in an embodiment of the present invention, Figure 7 shows a B-B cross-sectional view of Figure 6, Figure 8 shows the present invention In one embodiment, a schematic view of the front side of a pressure plate assembly of a detection device for turbidimetric detection in a closed state is shown in Figure 9, a cross-sectional view along line A-A of Figure 8. According to an embodiment of the present invention, the slot front shell 8 includes Guide groove 801, the guide groove 801 has an inclined slope.

The pressure plate assembly 10 includes a gas nozzle 1002, and a pressure plate 1001 is fixed above the gas nozzle 1002. The pressure plate 1001 includes a first segment structure 1008 and a second segment structure 1009. The first segment structure 1008 and the second segment structure 1009 form an "L" shaped structure.

The first segment structure 1008 is fixed to the air nozzle 1002. The end of the first segment structure 1008 is connected to the return spring 11, and is connected to the top of the slot front shell 8 through the return spring 11.

The second structure 1009 fixes the guide post 1007. When the microfluidic chip is inserted between the card slot front case 8 and the card slot rear case 9, the guide post 1007 is located on the side facing away from the front side of the base body 1 of the microfluidic chip.

The second segment structure 1009 fixes the positioning pin 1005. When the microfluidic chip is inserted between the card slot front shell 8 and the card slot rear shell 9, the positioning pin 1005 is located on the side facing the front side of the base body 1 of the microfluidic chip. The side of the second section structure 1009 facing the front side of the base body 1 of the microfluidic chip is flush with the end surface of the gas nozzle 1002 .

The guide post 1007 abuts the guide groove 801. When the pressure plate assembly 10 moves up and down along the front housing 8 of the slot, the guide post 1007 slides along the surface of the guide groove 801. Since the guide groove 801 has an inclined slope, when the guide post 1007 slides to the guide When the groove 801 is inclined, the pressure plate assembly 10 is driven to swing as a whole.

The limiting post 1006 is fixed below the air nozzle 1002. In a specific embodiment, the connecting component 1010 is fixed below the air nozzle 1002, and the connecting component 1010 fixes the limiting post 1006.

When the microfluidic chip moves downward between the front shell 8 of the card slot and the rear shell 9 of the card slot, the guide bar 104 of the microfluidic chip squeezes the limiting post 1006 and drives the pressure plate assembly 10 along the front shell 8 of the card slot. downward movement.

The air nozzle 1002 is provided with a ventilation channel 1003, and the ventilation channel 1003 is connected to a sealing ring 1004 provided on the end face of the air nozzle 1002.

With reference to Figures 6 to 9, when the microfluidic chip moves downward (presses the microfluidic chip downward) between the card slot front shell 8 and the card slot rear shell 9, the guide bar 104 of the microfluidic chip Squeeze the limiting column 1006 and push the limiting column 1006 to move downward, so that the entire microfluidic chip pressing plate assembly 10 moves downward along the front housing 8 of the card slot, and at the same time, the guide column 1007 slides to the inclined slope of the guide groove 801 , the pressure plate assembly 10 is driven to swing as a whole, so that the pressure plate assembly 10 swings toward the microfluidic chip as a whole, and is fastened to the front side of the microfluidic chip, and the pressure plate assembly 10 is in a closed state (as shown in Figure 9).

When the pressure plate assembly 10 is fastened to the front side of the microfluidic chip, the sealing ring 1004 abuts the second air hole 203 of the microfluidic chip, allowing the first air hole 105 of the base body 1 to pass between the second air hole 203 and the air nozzle 1002 The ventilation channel 1003 is connected to evacuate the interior of the base body 1 of the microfluidic chip.

When the pressure plate assembly 10 is fastened to the front side of the microfluidic chip, the positioning pin 1005 passes through the second positioning hole 202 of the microfluidic chip and is inserted into the first positioning hole 103 to prevent the microfluidic chip from moving upward away from the pressure plate assembly.

When the microfluidic chip moves upward (lifts the microfluidic chip upward) between the front housing 8 of the card slot and the rear housing 9 of the card slot, the return spring 11 pulls the first section 1008 of the pressure plate 1001 of the pressure plate assembly 10 , so that the entire pressure plate assembly 10 moves upward along the front shell 8 of the card slot, and at the same time, when the guide column 1007 slides to the inclined slope of the guide groove 801, the entire pressure plate assembly 10 is driven to swing, so that the entire pressure plate assembly 10 swings away from the microfluidic chip, and The front side of the microfluidic chip is separated, and the pressure plate assembly 10 is in an open state (as shown in Figure 7).

In the embodiment of the present invention, when the microfluidic chip is not inserted into the detection device, the pressure plate assembly 10 is pulled to the upper limit position by the return spring 11. Under the elastic force of the return spring 11, the pressure plate assembly 10 is in an open state.

In a preferred embodiment, the air nozzle 1002 is made of soft material, including but not limited to silicone, nitrile rubber, and fluorine rubber. The ventilation channel 1003 is connected to an external air source through an adapter for vacuuming.

As shown in Figure 10, a schematic diagram of the working state of a detection device for turbidimetric detection in one embodiment of the present invention is shown. According to the embodiment of the present invention, the mixing assembly 13 includes a progressive motor 1301 and a plurality of components located on the detection device. Permanent Magnet 1305.

The progressive motor 1301 drives multiple permanent magnets 1305 to reciprocate. Specifically, the mixing assembly 13 also includes a crank 1302, a connecting rod 1303 and a slider 1304. The multiple permanent magnets 1305 are installed on the slider 1304 and are implemented through a crank rocker mechanism. Multiple permanent magnets 1305 reciprocate.

When the pressure plate assembly 10 is fastened to the front side of the microfluidic chip, the plurality of light sources 12 are located on the front side of the microfluidic chip and correspond to the multiple observation chambers of the microfluidic chip. The plurality of detectors 14 are located on the front side of the microfluidic chip. The back side of the core and corresponds to the multiple observation chambers of the microfluidic chip.

Furthermore, the front housing 8 of the card slot is provided with a plurality of openings, so that the plurality of light sources 12 pass through the front housing 8 of the card slot in sequence and illuminate multiple observation cavities. The card slot back shell 9 is provided with a plurality of openings, so that the plurality of detectors 14 receive the light passing through the multiple observation cavities that pass through the card slot back shell 9 in sequence.

When the pressure plate assembly 10 is fastened to the front side of the microfluidic chip, a plurality of permanent magnets 1305 are located at the bottom of the microfluidic core and correspond to multiple observation cavities of the microfluidic chip. The progressive motor 1301 rotates and drives the crank 1302 to rotate. The crank 1302 drives the connecting rod 1303 to swing, thereby driving the slider 1304 to reciprocate in a linear motion, causing multiple permanent magnets 1305 to reciprocate. The multiple permanent magnets 1305 attract multiple components of the microfluidic chip respectively. The magnetic beads 5 in the observation chamber move back and forth in a straight line, stirring the reagent balls and magnetic beads in multiple observation chambers to achieve uniform mixing of the reagents.

As shown in Figure 11, a schematic diagram of the working state of a microfluidic chip used for turbidimetric detection in one embodiment of the present invention is shown. When the pressure plate assembly 10 is fastened on the front side of the microfluidic chip, the air nozzle 1002 passes through the ventilation The channel 1003 is evacuated, and the sample to be measured in the blood collection tube is moved along the third channel 106, the second channel 122, the first channel 107, and multiple channels (the fourth channel 113, the fifth channel 114, the sixth channel 115, the seventh channel Channel 116) is drawn into multiple observation chambers (shown by the solid arrows in Figure 11).

The gas in the multiple observation chambers flows along multiple airways (the third airway 117 , the fourth airway 118 , the fifth airway 119 , and the sixth airway 120 ), as well as the second airway 108 and the first airway 121 , the first air hole 105 is extracted by the air nozzle 1002 (shown by the dotted arrow in Figure 11). Since the filter element 6 can pass air but cannot pass liquid, the liquid is blocked to the filter element after filling multiple observation chambers. Then the mixing component 13 mixes the reagents, and after the mixing is completed, the light source 12 and the detector 14 are started for turbidity observation.

The present invention proposes a microfluidic chip and a detection device for turbidimetric detection. When the microfluidic chip performs turbidimetric detection, it only needs to vacuum through the pores to suck the tested sample into multiple observation chambers. For testing, the sample addition process is completed in one step, greatly reducing the sample addition steps.

Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention, but not to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: The technical solutions described in the foregoing embodiments can still be modified, or some or all of the technical features can be equivalently replaced; and these modifications or substitutions do not deviate from the essence of the corresponding technical solutions from the technical solutions of the embodiments of the present invention. scope.

Claims (8)

1. A detection device for nephelometry detection, the detection device comprising: the microfluidic chip is inserted between the clamping groove front shell and the clamping groove rear shell for turbidimetry detection;

the microfluidic chip includes: the device comprises a matrix, wherein a blood collection tube cavity is formed in the matrix, guide strips are arranged on two sides of the matrix, and a plurality of observation cavities are formed in the bottom of the matrix;

the plurality of observation cavities are communicated with a first channel through a plurality of channels, the first channel is communicated with a second channel, the second channel is communicated with a third channel, the third channel is communicated with the blood sampling tube cavity,

the front side of the matrix is provided with a first air hole, the first air hole is communicated with a first air passage, the first air passage is communicated with a second air passage, and the second air passage is communicated with a plurality of observation cavities through a plurality of air passages;

the front side cover plate and the rear side cover plate are respectively bonded on the front side and the rear side of the substrate, the front side cover plate is provided with a second air hole corresponding to the first air hole, and when the front side cover plate is covered on the substrate, the first air hole is communicated with the second air hole;

reagent balls and magnetic beads are respectively preset in the plurality of observation cavities;

the clamping groove front shell is provided with a pressing plate assembly, a return spring is arranged between the clamping groove front shell and the pressing plate assembly, and the pressing plate assembly is configured to move up and down along the clamping groove front shell and swing back and forth;

the clamping groove front shell comprises a guide groove which is provided with an inclined plane,

the pressing plate assembly comprises an air tap, a pressing plate is fixed above the air tap, the pressing plate comprises a first section structure and a second section structure, the first section structure is fixed with the air tap, the second section structure is fixed with a guide post, and the guide post is abutted with the guide groove;

a limiting column is fixed below the air tap, and when the microfluidic chip moves downwards between the clamping groove front shell and the clamping groove rear shell, the guide strip of the microfluidic chip extrudes the limiting column to drive the pressing plate assembly to move downwards along the clamping groove front shell;

when the microfluidic chip moves downwards between the clamping groove front shell and the clamping groove rear shell, the microfluidic chip extrudes the pressing plate assembly to move downwards along the clamping groove front shell, and meanwhile the pressing plate assembly swings towards the microfluidic chip and is buckled on the front side of the microfluidic chip;

when the microfluidic chip moves upwards between the clamping groove front shell and the clamping groove rear shell, the return spring pulls the pressing plate assembly to move upwards along the clamping groove front shell, and meanwhile the pressing plate assembly swings back to the microfluidic chip and is separated from the front side of the microfluidic chip.

2. The device according to claim 1, wherein a puncture needle having a hollow interior is provided in the blood collection lumen, and the puncture needle communicates with the third channel.

3. The microfluidic chip for nephelometry detection of claim 1, wherein the second channel is provided with a liquid inlet infrared detection point.

4. The detection apparatus according to claim 1, wherein the plurality of observation chambers includes a first observation chamber, a second observation chamber, a third observation chamber, and a fourth observation chamber;

the first observation cavity is communicated with the first channel through a fourth channel, the second observation cavity is communicated with the first channel through a fifth channel, the third observation cavity is communicated with the first channel through a sixth channel, and the fourth observation cavity is communicated with the first channel through a seventh channel.

5. The device of claim 4, wherein the first viewing chamber is in communication with the second airway via a third airway, the second viewing chamber is in communication with the second airway via a fourth airway, the third viewing chamber is in communication with the second airway via a fifth airway, and the fourth viewing chamber is in communication with the second airway via a sixth airway.

6. The test device of claim 5, wherein the third air path provides a first infrared test point, the fourth air path provides a second infrared test point, the fifth air path provides a third infrared test point, and the sixth air path provides a fourth infrared test point.

7. The detection device for nephelometry detection according to claim 1, wherein the air tap is provided with an air channel, and the air channel is communicated to the end face of the air tap to be provided with a sealing ring;

when the pressing plate component is buckled on the front side of the microfluidic chip, the sealing ring is abutted to the second air hole of the microfluidic chip.

8. The detection apparatus for nephelometry detection of claim 1, further comprising a blending assembly, a plurality of light sources, and a plurality of detectors, the blending assembly comprising a stepper motor, and a plurality of permanent magnets positioned at the detection apparatus;

when the pressing plate component is buckled on the front side of the microfluidic chip, the light sources are positioned on the front side of the microfluidic chip and correspond to the observation cavities of the microfluidic chip, and the detectors are positioned on the rear side of the microfluidic chip and correspond to the observation cavities of the microfluidic chip;

the stepping motor drives the permanent magnets to reciprocate to stir the reagent balls and the magnetic beads in the observation cavities.