CN115670941A - Functional suspension modeling crystal ball and manufacturing method thereof - Google Patents

Abstract

The invention provides a functional suspension modeling crystal ball, which comprises: the main body structure and the active ingredient, the active ingredient is filled in the main body structure or attached outside the main body structure. In addition, the present invention provides a method for manufacturing a functional suspension-molded crystal ball, comprising: providing a mould, wherein the mould is provided with a forming hole; adding the crystal ball combined liquid into a forming hole of a mould; and forming the functional suspension modeling crystal ball by the crystal ball combination liquid in the molding cavity through the molding combination liquid, wherein the functional suspension modeling crystal ball comprises a main body structure and an active ingredient, and the active ingredient is filled in the main body structure or attached to the outside of the main body structure. The functional suspension modeling crystal ball has the advantage of protecting the active ingredients to avoid deterioration of the active ingredients due to contact with the environment.

Description

Functional suspended molding crystal ball and manufacturing method thereof

technical field

The invention relates to a suspended crystal ball and a manufacturing method thereof, in particular to a functional suspended crystal ball and a manufacturing method thereof.

Background technique

Cosmetics manufacturers usually make active ingredients into visible dosage forms, such as crystal balls, water-in-oil, or oil-in-water, in order to allow consumers to observe the existence of active ingredients with naked eyes or as a sales gimmick. However, most of the manufacturing methods in the prior art use micro-channel devices, which leads to high manufacturing costs.

For example, the Taiwan Utility Model Patent Publication No. M466662 proposed "a collagen microliposphere structure that promotes skin firmness, whiteness and translucence, and increases skin elasticity", which uses micro-channel and film pressurized injection technology to manufacture Lipogen collagen Proteolipospheres. Microchannel technology is to use water to squeeze out the oil droplets near the outlet through the oily solvent dissolved in lipids, forming small protrusions at the outlet of the channel, cutting off the vacuoles at the right time, and continuously producing collagen of uniform size Protein lipospheres; film pressurized injection technology uses a filter membrane with an appropriate pore size to obtain the best pressurized filtration pressure and flow rate to produce collagen lipospheres of uniform size.

For another example, the "Structural Color Material and Cosmetics Using It" proposed by Taiwan Invention Patent Publication No. 201400556, the structural color material is composed of resin particles with structural color, and the resin particles are in a tiny tubular flow channel, with a certain amount of color. The flow rate is injected into the fluid mixed with the water medium containing the water-soluble radical polymerization initiator and the radical polymerizable monomer, and after the fluid temperature reaches the preset temperature instantly, in the presence of the water-soluble radical polymerization initiator, It is obtained by reacting a part of the free radical polymerizable monomer so that the particle core of uniform resin particles is formed in the tiny tubular flow channel, and then using a reactor with a stirring device to polymerize the polymerization reaction solution containing the obtained particle core For the resin particles, the average particle diameter of the resin particles is in the range of 150 to 400 nm, and the absolute value of the zeta potential is in the range of 30 to 80 mV.

For another example, the "device for manufacturing particles of equal particle size" proposed by Taiwan Utility Model Patent Publication No. M421160 includes a base with a space, and the base is respectively provided with a first flow channel and a second flow connected to the space. channel, and the base is provided with an output flow channel that collects the first flow channel and the second flow channel. There is a push rod sliding in the space, and the push rod is provided with through holes corresponding to the first flow channel and the second flow channel; the push rod is driven to slide laterally in the space by the driving member, so that the through holes of the push rod are sequentially Circular alignment of the first flow channel and the second flow channel, so that the raw materials can be separated by gas to produce particles of equal particle size, and used for skin care products.

Therefore, it is indeed one of the problems that people in the technical field of the present invention actively solve for the problems existing in the visible dosage form cosmetics of the prior art.

Contents of the invention

An object of the present invention is to provide a suspended crystal ball, which can protect the active ingredient and prevent the active ingredient from deteriorating due to contact with the environment.

Another object of the present invention is to provide a suspended crystal ball, which can be suspended at the interface between two liquid layers, so that consumers can observe the active ingredient with naked eyes.

Another object of the present invention is to propose a suspended crystal ball, which can be mixed with two liquids, and quickly forms an interface between the two liquid layers as the liquids are layered, so as to reduce the contact time with the environment.

Another object of the present invention is to provide a suspended crystal ball, which can be prepared not only by microchannel equipment but also by other non-microchannel equipment, thereby reducing the production cost.

Therefore, the present invention proposes a functional suspension molding crystal ball, which includes: a main structure and an active ingredient, wherein the active ingredient is filled in the main structure or attached to the outside of the main structure.

Preferably, the specific gravity of the functional suspended crystal ball is 0.95-1.

Preferably, the main structure contains sodium hyaluronate, polyacrylic acid, sodium alginate, xanthan gum, sclerotium gum, hydroxyethyl cellulose ( hydroxyethyl cellulose), and guar gum (guar gum).

Preferably, the active ingredient is a plant extract.

According to the present invention, the functional suspended shaped crystal ball is suspended in the interface between two liquid layers in normal state. When using, you can first shake the functional suspended crystal ball and the two liquid layers to mix, and then take out the functional suspended crystal ball; after that, put the functional suspended crystal ball and the two liquids to let the liquid layer and then form in The interface between the two liquid layers, thereby reducing the contact time with the environment, thereby avoiding the deterioration of the main structure or active ingredients. In addition, by filling the active ingredient in the main structure or adhering to the outside of the main structure, the active ingredient can be protected from deterioration due to contact with the environment.

On the other hand, the present invention proposes a method for manufacturing functional suspended molding crystal balls, which includes: providing a mold, the mold has a forming cavity; adding the crystal ball combination liquid into the forming cavity of the mold; The crystal ball combination liquid in the hole forms a functional suspension modeling crystal ball, and the functional suspension modeling crystal ball includes a main structure and an active ingredient, and the active ingredient is filled in the main structure or attached to the outside of the main structure.

Preferably, the crystal ball combination liquid contains solvent, sodium hyaluronate, polyacrylic acid, sodium alginate, sanxian gum, sclerotinia, hydroxyethyl cellulose, guar gum, and active ingredients.

Preferably, based on the total weight of the crystal ball combination liquid, the content of sodium hyaluronate is 0.01 to 5 wt%, the content of polyacrylic acid is 0.5 to 5 wt%, the content of sodium alginate is 0.5 to 5 wt%, and the content of Sanxian gum 0.5 to 5% by weight, 0.5 to 2% by weight of sclerotinia, 0.04 to 2% by weight of hydroxyethyl cellulose, 0.04 to 2% by weight of guar gum, and 0.05 to 5% by weight of active ingredient %, and the content of solvent is to make up to 100wt%.

Preferably, the pH value of the crystal ball combination liquid is pH4-8.

Preferably, the forming composition liquid contains solvent, calcium carbonate, calcium lactate, calcium chloride, barium chloride, and potassium chloride.

Preferably, based on the total weight of the forming combination liquid, the content of calcium carbonate is 0.5 to 2wt%, the content of calcium lactate is 0.5 to 2wt%, the content of calcium chloride is 0.5 to 2wt%, and the content of barium chloride is 0.5 to 2wt%, the content of potassium chloride is 0.5 to 2wt%, and the content of solvent is supplemented to 100wt%.

Preferably, the pH of the forming composition liquid is pH4-8.

Preferably, before adding the crystal ball combination liquid into the forming cavity, the forming cavity of the mold can be wetted with the forming combination liquid.

In yet another aspect, the present invention provides a composition, which comprises: a first liquid layer, a second liquid layer, and the above-mentioned functional suspended crystal balls, and there is formed between the first liquid layer and the second liquid layer The interface, while the functional floating shape crystal ball is suspended on the interface.

Preferably, the first liquid layer contains solvent, glycerin, active ingredients, and alcohols.

Preferably, the second liquid layer contains cyclopentasiloxane (cyclopentasiloxane), polydimethylsiloxane (polydimethylsiloxane), phenylpropyldimethylsiloxysilicate (phenylpropyldimethylsiloxysilicate), and cetearyl Cetearyl methicone.

Preferably, based on the total weight of the first liquid layer, the content of glycerin is 5 to 20 wt%, the content of active ingredients is 0.1 to 20 wt%, the content of alcohols is 1 to 10 wt%, and the content of solvent is supplemented to 100% by weight.

Preferably, based on the total weight of the second liquid layer, the content of cyclopentasiloxane is 5 to 40 wt%, the content of polydimethylsiloxane is 1 to 20 wt%, phenylpropyldimethylsiloxane The content of the base silicate is 1 to 20 wt%, and the content of the cetearyl polymethicone is 1 to 20 wt%.

Preferably, the specific gravity of the first liquid layer is 1 to 1.5.

Preferably, the specific gravity of the second liquid layer is 0.6 to 0.95.

Description of drawings

FIG. 1 is a schematic flowchart illustrating a manufacturing method according to an embodiment of the present invention.

FIG. 2 is a structural schematic diagram illustrating a mold used in an embodiment of the present invention.

Fig. 3 is a photograph showing the functional suspended crystal ball obtained in the embodiment of the present invention.

Fig. 4 is a photograph showing the interface between two liquid layers in which the functional suspended shaped crystal ball obtained by the embodiment of the present invention is suspended.

FIG. 5 is a schematic flow diagram illustrating a manufacturing method of another embodiment of the present invention;

Figure 6 is a histogram illustrating the effect of AMLE on the activity of B16F10 cells;

Fig. 7A is a photographic diagram illustrating the protective effects of AMLW and AMLE on DNA;

Figure 7B is a histogram illustrating the protective effects of AMLW and AMLE on DNA;

Figure 8 is a histogram illustrating the protective effect of the thorny onion extract on DNA;

Fig. 9 is a histogram illustrating the effect of thorny onion extract on the activity of B16F10 cells;

Fig. 10 is a histogram illustrating the inhibitory effect of the Allium japonicus extract on tyrosinase activity;

Fig. 11 is a photogram illustrating the performance of PCNA after being acted on by PSB;

Fig. 12 is a photo graph illustrating the expression of Lamin A protein in cells after being treated with PSBL.

Detailed ways

In order to make the above and/or other purposes, functions, and features of the present invention more obvious and understandable, the preferred implementation modes are specifically cited below, and are described in detail below:

Please refer to FIG. 1 , an embodiment of the present invention proposes a method for manufacturing functional suspended crystal balls. As shown in Figure 3, the prepared functional suspended crystal ball has a main structure and an active ingredient. The active ingredient is filled in the main structure or attached to the outside of the main structure to protect the active ingredient from deterioration caused by contact with the environment. As shown in FIG. 4 , the prepared functional suspended crystal balls can be combined with the first liquid layer and the second liquid layer to form a composition and suspended in the interface formed between the first liquid layer and the second liquid layer. Based on the total weight of the first liquid layer, the first liquid layer may contain 5 to 20 wt% of glycerin, 0.1 to 20 wt% of active ingredients, 1 to 10 wt% of alcohols (such as: propylene glycol (propyleneglycol), butylene glycol (butylene glycol) glycol), or a mixture thereof), and a solvent (such as water) supplemented to 100wt%, but not limited thereto; and the specific gravity of the first liquid layer may be 1 to 1.5, but not limited thereto. Based on the total weight of the second liquid layer, the second liquid layer may contain 5 to 40 wt% of cyclopentasiloxane, 1 to 20 wt% of polydimethylsiloxane, 1 to 20 wt% of phenylpropyldimethylsiloxane Siloxysilicate, and 1 to 20 wt% of cetearyl polymethicone, but not limited thereto; and the specific gravity of the second liquid layer is 0.6 to 0.95, but not limited thereto . When in use, the composition can be shaken first to mix the functional suspended crystal balls with the two liquid layers, and then the functional suspended crystal balls can be taken out; after that, the functional suspended crystal balls and the two liquids can be left to stand to make the two liquids layer again The first liquid layer and the second liquid layer are formed, and the functional suspended crystal ball is formed at the interface between the two liquid layers, thereby reducing the contact time of the functional suspended crystal ball with the environment, thereby avoiding the main structure or activity Ingredients spoiled. The detailed steps related to the manufacturing method of this embodiment are as follows.

Firstly, a mold (1) is provided, and the mold has a forming cavity (11) and two openings communicating with the forming cavity (11) (step S11). Generally speaking, the shape of the forming hole (11) can determine the shape of the functional suspended molding crystal ball. As shown in Figure 2, when the external shape of the forming hole (11) is heart-shaped, the external shape of the functional suspended molding crystal ball is of course heart-shaped. It is understandable that the shape of the forming cavity (11) can also be circular, triangular, or star-shaped, but not limited thereto.

Next, place one of the openings of the mold (1) in the forming composition liquid (3), and make the forming composition liquid (3) wet the forming cavity (11) of the mold (1) (step S12). It should be noted that the purpose of wetting the forming cavity (11) is to facilitate the removal of the subsequently obtained functional suspension shaped crystal balls from the forming cavity (11) without remaining in the mold (1). Based on the total weight of the forming combination liquid (3), the forming combination liquid (3) can contain 0.5 to 2wt% calcium carbonate, 0.5 to 2wt% calcium lactate, 0.5 to 2wt% calcium chloride, 0.5 to 2wt% chlorine Barium chloride, 0.5 to 2wt% potassium chloride, and a solvent (such as water) supplemented to 100wt%, but not limited thereto; and the pH value of the forming combination liquid (3) can be pH4 to 8, but not This is the limit.

Finally, add the crystal ball combination liquid (2) into the forming cavity (11) of the mold (1), so that the forming function of the crystal ball combination liquid (2) can pass through the forming cavity (11) Suspension modeling crystal ball (4) (steps S13, S14). Based on the total weight of the crystal ball combination liquid (2), the crystal ball combination liquid (2) may contain 0.01 to 5wt% sodium hyaluronate, 0.5 to 5wt% polyacrylic acid, 0.5 to 5wt% sodium alginate, 0.5 to 5wt% Sanxian gum, 0.5 to 2wt% sclerotin, 0.04 to 2wt% hydroxyethyl cellulose, 0.04 to 2wt% guar gum, 0.05 to 5wt% active ingredient, and a solvent that is supplemented to 100wt% (such as: water), but not limited thereto; and the pH value of the crystal ball combination liquid (2) may be pH 4 to 8, but not limited thereto. In addition, an active ingredient can be defined as an ingredient that has one of the functions of promoting skin protection and repair, anti-wrinkle, anti-oxidation, anti-inflammation, anti-aging, and whitening, and is preferably a plant extract. For example, the plant extract can be the Cinnamomum camphora germ tissue extract described in Taiwan Invention Patent Publication No. I697332, the orchid germ tissue extract described in Taiwan Invention Patent Publication No. 202021609, and the Chinese Taiwan Invention Patent Publication No. 202023527. Curculigo grass extract described above, longan flower extract described in Chinese invention patent publication number CN109276493A, or red quinoa extract described in Chinese invention patent publication number CN108852924A, but not limited thereto, and the full text of these documents is hereby incorporated for reference. It should be noted that since the molding combination liquid (3) can polymerize the crystal ball combination liquid (2) to form a functional suspended crystal ball (4) including the main structure and active ingredients, the main structure can contain sodium hyaluronate, polyacrylic acid , sodium alginate, sanxian gum, sclerotinia, hydroxyethyl cellulose, and guar gum, but not limited thereto. In addition, the specific gravity of the functional suspension molding crystal ball (4) may be 0.95 to 1, but not limited thereto.

Please also refer to FIG. 5 , another embodiment of the present invention proposes a method for manufacturing a functional suspended crystal ball. Unless otherwise specified, the meanings expressed by the terms of this embodiment are in principle the same as the meanings expressed by the terms of the previous embodiment. The detailed steps related to the manufacturing method of this embodiment are as follows.

First, a mold (1) is provided, which has a forming cavity (11) and an opening communicating with the forming cavity (11) (step S21).

Next, the crystal ball combination liquid (2) is added into the forming cavity (11) of the mold (1) through the opening (step S22). In addition, as mentioned above, in order to remove the subsequently obtained functional suspended crystal balls from the forming cavity (11) without remaining in the mold (1), before adding the crystal ball combination liquid (2), you can The molding cavity (11) of the mold (1) is wetted with the molding combination liquid.

Finally, add the forming combination liquid (3) to the crystal ball combination liquid (2) in the forming cavity (11) to penetrate the forming combination liquid (3) in the forming cavity (11) to make the crystal balls in the forming cavity (11) The combination solution (2) forms a functional suspended crystal ball (4) (steps S23, S24).

More preferably, the plant extract is soursop leaf extract. In a preferred embodiment, the preparation method of the soursop leaf extract comprises: first, washing the soursop leaves, drying them in the shade for about 3 to 5 days, and then cutting the soursop leaves into small pieces About 0.5cm ² size for extraction. Wherein, the extraction rate formula of the present invention is: (extract weight/sample original total weight)×100%. Next, soak the shredded soursop leaves. This step includes two implementations, wherein the first implementation is "ultrasonic low-temperature water extraction". Specifically, its implementation is : Weigh about 100g of soursop leaves, soak them in 900ml of deionized water at 4°C, and use ultrasonic vibration extraction equipment to continuously extract at low temperature with a total energy of 300-600w. In a preferred embodiment, the continuous extraction is shaken for 3 minutes and rested for 2 minutes for a total of 60 minutes. In another preferred embodiment, after the continuous extraction is completed, centrifuge at a high speed of 15,000 rpm for 30 minutes at a temperature of 4°C, collect the supernatant, and then filter it through a vacuum filter to obtain a clarified filtrate. The concentrator and freeze dryer concentrate the semi-finished extract to make the soursop leaf water extract. In yet another preferred embodiment, the freeze-dried product of the water extract of soursop leaves is stored at -80°C; the second embodiment is "ultrasonic low-temperature ethanol extraction method", specifically, the implementation method is: About 100g of soursop leaves were weighed and soaked in five times the volume of 95% ethanol, kept in the dark at 4°C for three days, and extracted continuously at low temperature with a total energy of 300-600w. In a preferred embodiment, the continuous extraction is shaken for 3 minutes and rested for 2 minutes for a total of 60 minutes. In another preferred embodiment, after the continuous extraction is completed, centrifuge at a high speed of 15,000 rpm for 30 minutes at a temperature of 4°C, collect the supernatant, and then filter it through a vacuum filter to obtain a clarified filtrate. The concentrator and freeze dryer will remove the solvent from the semi-finished extract to produce the ethanol extract of soursop leaves. In yet another preferred embodiment, the lyophilized product of the ethanol extract of soursop leaves is stored at -80°C. The water extract of soursop leaves is abbreviated as AMLW below: the ethanol extract of soursop leaves is abbreviated as AMLE.

As shown in Figure 6, it is the viability of B16F10 cells when the ethanol extract of soursop leaves acts on melanoma cells (B16F10 cells) at 50-1000 μg/ml. Black tumor cells (B16F10) cells have significant toxicity, and the cell viability is only 30%, which means that the ethanol extract of soursop leaves has the effect of poisoning and killing cancer cells, and can be used to prepare medicines, cosmetics, Raw materials for skin care products, body cleansing products, or fragrances.

As shown in Figures 7A to 7B, it is the DNA pUC119 test that has been irradiated by UVB. When the DNA is damaged, the DNA will break into a linear structure (linear-form, L-form) from a circular surrounding structure (supercoiled-form, S-form). ), using the difference in the moving speed of nucleic acids with different molecular weights in the colloidal pore size, the pUC19 DNA was induced by H ₂ O ₂ (1mM) and irradiated with UVB (20mJ/cm ² ). The position of the S-form, and when damaged, the DNA will appear in the position of the L-form. As shown in Figures 7A to 7B, it can be found that the control group is not damaged and can maintain a complete DNA pattern and present a circular structure. In the UVB damage group without drugs, the DNA structure is damaged and presents a linear structure, and AMLS is added first. Or AMLW can maintain a relatively complete DNA surrounding structure when it is irradiated by UVB. Therefore, it can be found that the extract of soursop leaves has the effect of protecting DNA, and the water extract of soursop leaves has a protective effect on DNA. The effect is better than ethanol extract.

More preferably, the plant extract is the thorn stem extract. In a preferred embodiment, the preparation method of the thorny onion stem extract is the same as that of the soursop leaf extract, so I won’t go into details here, and the “ultrasonic low-temperature water extraction method” is used to prepare An aqueous extract of the scallion stalk; the ethanol extract of the scallion stalk is prepared by the "ultrasonic low-temperature ethanol extraction method". In another preferred embodiment, the plant extract is the leaf extract of Allium japonicus. In yet another preferred embodiment, the preparation method of the thorn leaf extract is the same as the preparation method of the soursop leaf extract, and will not be repeated here, wherein the "ultrasonic low-temperature water extraction method" is used to prepare Aqueous extract of thorny allium leaves; use the "ultrasonic low-temperature ethanol extraction method" to make thorny allium leaf ethanol extract. The thorny allium leaf water extract is abbreviated as ZLLW below: the thorny allium leaf ethanol extract is abbreviated as ZLLE;

As shown in Figure 8, it is an experiment of the DNA protection effect of the extract of the thorn onion, in which: in the group that was only damaged by H2O2, FeSO4 and UVB, the DNA structure was damaged, showing a large number of linear structures, while the DNA structure was damaged by the thorn The group of onion stem water extracts can maintain the integrity of DNA and present a circular winding structure, which has an excellent DNA protection effect.

As shown in Figure 9, it is the viability of B16F10 cells when the Allium pilaris extract acts on melanoma cells (B16F10 cells) with 20～1000 μ g/ml, wherein: provide control group, its B16F10 cell activity is 100%, when adding Under the action of ZLLE at a concentration of 100 μg/ml and above, the survival rate of B16F10 cells was lower than 80%; when ZLSE was added at a concentration of 250 μg/ml and above, the survival rate of B16F10 cells was lower than 80%, showing that it has a positive effect on B16F10 cells toxicity. Indicates that the ethanol extract of thorny onion leaves or ethanol extract of thorny onion stems has the effect of poisoning and killing cancer cells, and can be used as raw materials for the preparation of medicines, cosmetics, skin care products, body cleaning products, or fragrances for the treatment of skin cancer .

As shown in Figure 10, ZLSE, ZLLE, and ZLLW significantly promote the ability of tyrosinase, leading to an increase in melanin production, and the increase rate tends to increase with the increase in concentration, so it is speculated that ZLSE, ZLLE, and ZLLW have the ability to promote melanin production It can be used to prepare compositions for promoting melanin production or strengthening hair roots, and the prepared compositions can be used as raw materials for products such as self-tanning preparations, scalp care products, shampoos, oral preparations, food or hair tonic.

More preferably, the plant extract is the growth point tissue extract of peony stamens. In a preferred embodiment, the extract of growing point tissue of peony stamens comprises aqueous extract of growing point tissue of peony stamens or ethanol extract of growing point tissues of peony stamens. In another preferred embodiment, the preparation method of the water-extracted extract of peony stamen growth point tissue comprises: taking fresh peony flower stamens, freezing and solidifying the peony stamen tissue with liquid nitrogen at -195.79°C (77K) rapidly, and freezing The grinder grinds the peony stamen tissue, and grinds the peony stamen tissue into powder; extracts the pure water and the peony stamen tissue powder with a weight ratio of 9:1, and soaks the peony stamen tissue powder in pure water, at a temperature of 4°C to 50 Soak for 72-120 hours at ℃; extract the mixture of soaked peony flower stamen powder and pure water under the temperature condition of -4℃～25℃, with ultrasonic energy of 500-800w for continuous and rapid extraction , in which, shake continuously for 5 minutes and rest for 2 minutes until the total shaking time reaches 60-120 minutes; centrifuge at a high speed of 15,000g for 10 minutes under the temperature condition of -20°C to remove the bottom sediment and collect the supernatant ; After the supernatant is filtered with a microporous structure membrane with a pore size of 0.22 to 0.45 μM, it is filtered by a vacuum screening device to obtain a clarified filtrate; the clarified filter is obtained by using a decompression concentrator and a vacuum freeze dryer. Liquid freeze-dried to obtain the peony stamen growing point tissue extract (PSB). In yet another preferred embodiment, the preparation method of the ethanol extract of peony stamen growth point tissue comprises: taking fresh peony flower stamens, freezing and solidifying the peony stamen tissue with liquid nitrogen at -195.79°C (77K) rapidly, and freezing The grinder grinds the peony stamen tissue, and grinds the peony stamen tissue into powder; extracts the alcohol solution (containing 50% to 75% by weight of ethanol) and the peony stamen tissue powder with a weight ratio of 9:1, and soaks the peony stamen tissue powder in In the alcohol solution (containing 50% by weight to 75% by weight of ethanol), soak for 72 to 120 hours at a temperature of 4°C to 50°C; weight % ethanol) mixture, under the temperature condition of -4 ° C ~ 25 ° C, the ultrasonic energy with a total energy of 500 ~ 800w is continuously and rapidly oscillating to extract, wherein, the mode of shaking for 5 minutes and resting for 2 minutes is continuously oscillating until The total shaking time is 60-120 minutes; centrifuge at a high speed of 15,000g for 10 minutes under the temperature condition of -20°C to remove the bottom sediment and collect the supernatant; the supernatant is made into a microporous structure with a pore size of 0.22-0.45μm After filtering the membrane, use a vacuum screener to filter to obtain a clear filtrate; use a vacuum concentrator and a vacuum freeze dryer to freeze-dry the clarified filtrate to obtain a peony stamen growth point tissue extract (PSB). Hereinafter, the peony stamen growth point tissue extract is referred to as PSB for short.

PCNA (proliferating cell nuclear antigen) is a proliferating cell nuclear antigen that can act as a DNA clamp to help DNA polymerase activity (DNA pol) and then recruit FEN1 (Flap endonuclease 1) to remove the overhanging 5' end, which is DNA repair The final step involves DNA ligase, the enzyme that holds the final DNA strands together in phosphodiester bonds. There are a number of mechanisms to repair damaged duplexes. As shown in FIG. 11 , in the control group without UVB damage, the performance of PCNA was quantitative (1.0), but after UVB irradiation, its performance was significantly reduced (0.2). The cells treated with peony stamen growth point tissue extract (PSB) can significantly improve the expression of PCNA (0.8). It shows that the growth point tissue extract of peony stamen can effectively regulate and repair skin cells from UV damage.

More preferably, the active ingredient is the compound microcrystalline capsule composition of peony stamens. In a preferred embodiment, the preparation method of the peony stamen composite microcrystalline capsule composition comprises: mixing the peony stamen growth point tissue extract (PSB) with carrier materials, and dispersing the carrier materials to form a colloidal complex. Specifically, the carrier material is dissolved in a solvent, and the solvent is removed by a rotary decompression concentrator to form a lipid film, and then the peony stamen growth point tissue extract (PSB) is added to the lipid film to disperse the lipid into a colloidal complex , the obtained colloid complex is the peony stamen composite microcrystalline capsule composition. Hereinafter, the peony stamen compound microcrystalline capsule composition is referred to as PSBL for short.

Mutations in the lamin A gene can impair DNA repair function and destabilize the genome, leading to premature aging. The anti-aging mechanism found that Lamin A protein can stimulate the longevity gene SIRT1, so that cells can self-protect, self-renew and delay aging. To evaluate the performance of coated peony stamen microcapsules (PSBL) to regulate the expression of anti-aging protein Lamin A, thereby delaying cellular senescence. Human skin keratinized cells (HaCaT cells) (1.5×105cell/mL) were cultured in a 10cm-diameter dish (dish), cultured in a 37°C and 5% CO2 incubator for at least 24 hours, and added coated peony flower capsule (PSBL), placed in an incubator for 24 hours. When the reaction time is up, use RIPA buffer for protein extraction, and then perform SDS-PAGE electrophoresis until the target protein is separated. Add 0.5% skimmed milk for 2 minutes, then add level 1 antibody to react for 10 minutes, wash with 0.1% phosphate buffered saline (PBST) for 3 times, then add level 2 antibody for 10 minutes, then wash with 0.1% PBST for 3 times . Finally, the ECL luminescence sensing reagent was added, and a luminescence camera system was used to take pictures, and image J was used to quantitatively analyze protein expression. As shown in Figure 12, after the human skin keratinized cells (HaCaT cells) were treated with coated peony stamen microcapsules (PSBL), the expression level of Lamin A protein in the cells was analyzed. Cytoskeletal protein β-actin was used as a quantitative control group. The results showed that compared with the control group (Lamin A was 1.0), the expression of Lamin A increased to 3.8 after the cells were treated with coated peony stamen microcapsules (PSBL), indicating that the coated peony stamen microcapsules (PSBL) could activate Lamin A protein, it is speculated that it has anti-aging effect.

But the above-mentioned person is only the preferred embodiment of the present invention, but can not limit the patent protection scope of the present invention with this; Modifications still fall within the scope of patent protection of the present invention.

Claims (10)

1. A functional suspended molding crystal ball, characterized in that it comprises: main structure; and The active ingredient is filled in the main structure or attached to the outside of the main structure. 2. The functional suspended molding crystal ball as claimed in claim 1, characterized in that: the main structure contains sodium hyaluronate, polyacrylic acid, sodium alginate, sanxian gum, sclerotin, hydroxyethyl cellulose, and guar gum. 3. The functional suspended crystal ball according to claim 1, wherein the active ingredient is a plant extract. 4. A method for manufacturing a functional suspended crystal ball, characterized in that it comprises: providing a mold having a forming cavity; adding the crystal ball combination liquid into the forming cavity of the mould; and Through the forming combination liquid, the crystal ball combination liquid in the forming cavity forms the functional suspension molding crystal ball, and the functional suspension molding crystal ball includes a main structure and an active ingredient, and the active ingredient is filled in the main structure or attached to the outside the main structure. 5. The manufacturing method according to claim 4, further comprising: before the step of adding the crystal ball combination liquid: Wet the molding cavities of the mold with the molding composition. 6. The manufacturing method according to claim 4, characterized in that: the forming combination liquid contains 0.5 to 2wt% calcium carbonate, 0.5 to 2wt% calcium lactate, 0.5 to 2wt% calcium chloride according to its total weight , 0.5 to 2wt% of barium chloride, 0.5 to 2wt% of potassium chloride, and a solvent to make up to 100wt%. 7. The manufacturing method according to claim 4, characterized in that: the crystal ball combination liquid contains 0.01 to 5wt% sodium hyaluronate, 0.5 to 5wt% polyacrylic acid, and 0.5 to 5wt% alginic acid based on its total weight Sodium, 0.5 to 5wt% sanxian gum, 0.5 to 2wt% sclerotinia, 0.04 to 2wt% hydroxyethylcellulose, 0.04 to 2wt% guar gum, 0.05 to 5wt% active ingredient, and Make up to 100 wt% solvent. 8. A composition, characterized in that, comprising: first liquid layer; a second liquid layer; and The functional suspended crystal ball according to any one of claims 1 to 3; An interface is formed between the first liquid layer and the second liquid layer, and the functional suspended crystal ball is suspended on the interface. 9. The composition according to claim 8, wherein the first liquid layer contains 5 to 20 wt% of glycerol, 0.1 to 20 wt% of active ingredients, 1 to 10 wt% of alcohols, And make up to 100wt% solvent, and the second liquid layer contains 5 to 40wt% cyclopentasiloxane, 1 to 20wt% polydimethylsiloxane, 1 to 20wt% benzene according to its total weight Propyldimethylsiloxysilicate, and 1 to 20% by weight of cetearyl methicone. 10. The composition according to claim 9, wherein the specific gravity of the functional suspended crystal ball is 0.95 to 1, the specific gravity of the first liquid layer is 1 to 1.5, and the specific gravity of the second liquid layer is 0.6 to 0.95.

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