CN115656531A - Integrated pesticide residue standardized rapid detection box and detection method - Google Patents
Integrated pesticide residue standardized rapid detection box and detection method Download PDFInfo
- Publication number
- CN115656531A CN115656531A CN202211569190.XA CN202211569190A CN115656531A CN 115656531 A CN115656531 A CN 115656531A CN 202211569190 A CN202211569190 A CN 202211569190A CN 115656531 A CN115656531 A CN 115656531A
- Authority
- CN
- China
- Prior art keywords
- detection
- cabin
- sample
- reagent
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 283
- 239000000447 pesticide residue Substances 0.000 title claims abstract description 39
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 157
- 238000005070 sampling Methods 0.000 claims abstract description 47
- 238000000034 method Methods 0.000 claims abstract description 30
- 239000007788 liquid Substances 0.000 claims description 105
- 239000007853 buffer solution Substances 0.000 claims description 22
- 239000000243 solution Substances 0.000 claims description 18
- 238000012360 testing method Methods 0.000 claims description 12
- 239000000575 pesticide Substances 0.000 claims description 11
- 238000007789 sealing Methods 0.000 claims description 11
- 230000001681 protective effect Effects 0.000 claims description 7
- 238000007689 inspection Methods 0.000 claims description 4
- 238000004026 adhesive bonding Methods 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 238000007731 hot pressing Methods 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 238000002798 spectrophotometry method Methods 0.000 claims description 3
- 239000012086 standard solution Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 2
- 238000000605 extraction Methods 0.000 abstract description 18
- 230000008569 process Effects 0.000 abstract description 15
- 238000011534 incubation Methods 0.000 abstract description 5
- 238000002156 mixing Methods 0.000 abstract description 5
- 238000006243 chemical reaction Methods 0.000 abstract description 4
- 230000003287 optical effect Effects 0.000 abstract description 4
- 238000012546 transfer Methods 0.000 abstract description 3
- RAHZWNYVWXNFOC-UHFFFAOYSA-N Sulphur dioxide Chemical group O=S=O RAHZWNYVWXNFOC-UHFFFAOYSA-N 0.000 description 11
- 102000004190 Enzymes Human genes 0.000 description 8
- 108090000790 Enzymes Proteins 0.000 description 8
- 229940088598 enzyme Drugs 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 5
- 230000005764 inhibitory process Effects 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- KXDHJXZQYSOELW-UHFFFAOYSA-M Carbamate Chemical compound NC([O-])=O KXDHJXZQYSOELW-UHFFFAOYSA-M 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 230000010355 oscillation Effects 0.000 description 3
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 2
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 2
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 2
- 241001506047 Tremella Species 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 235000012055 fruits and vegetables Nutrition 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 239000004005 microsphere Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 102000003914 Cholinesterases Human genes 0.000 description 1
- 108090000322 Cholinesterases Proteins 0.000 description 1
- 101001018064 Homo sapiens Lysosomal-trafficking regulator Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 102100033472 Lysosomal-trafficking regulator Human genes 0.000 description 1
- 244000038561 Modiola caroliniana Species 0.000 description 1
- 235000010703 Modiola caroliniana Nutrition 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 1
- OIPILFWXSMYKGL-UHFFFAOYSA-N acetylcholine Chemical compound CC(=O)OCC[N+](C)(C)C OIPILFWXSMYKGL-UHFFFAOYSA-N 0.000 description 1
- 229960004373 acetylcholine Drugs 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229940048961 cholinesterase Drugs 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- YNLAOSYQHBDIKW-UHFFFAOYSA-M diethylaluminium chloride Chemical compound CC[Al](Cl)CC YNLAOSYQHBDIKW-UHFFFAOYSA-M 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000011536 extraction buffer Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000003317 immunochromatography Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000007830 nerve conduction Effects 0.000 description 1
- AFAIELJLZYUNPW-UHFFFAOYSA-N pararosaniline free base Chemical compound C1=CC(N)=CC=C1C(C=1C=CC(N)=CC=1)=C1C=CC(=N)C=C1 AFAIELJLZYUNPW-UHFFFAOYSA-N 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 239000002096 quantum dot Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Images
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention relates to a detection box and a detection method for integrated standardized and rapid detection of pesticide residue, which comprises a detection box body, wherein a sampling cabin, a sample detection cabin and a comparison cabin are arranged in the detection box body; the detection box and the detection method are simple in operation, and can complete all detection operations of sample addition, sample extraction, sample transfer, reagent addition, uniform mixing, incubation reaction and detection contrast in the detection box only by a few simple external operations, and detection can be completed without professional operators and professional experimental environments; and the detection process is located in the detection box, the detection environment is stable, the influence of human factors on the detection process is reduced, the stability of the detection process is ensured, a sample detection cabin and a comparison cabin are arranged in the detection box for comparison, whether pesticide residues exist in the sample detection cabin can be detected quickly during optical detection, and the detection efficiency is high.
Description
Technical Field
The invention relates to the technical field of pesticide residue detection, in particular to a detection box and a detection method for integrated standardized and rapid detection of pesticide residue.
Background
The rapid detection of pesticide residues in vegetables and fruits is a very important work in the practical management and control of food safety at present. The traditional method for rapidly detecting pesticide residues mostly adopts a method of standard GB/T5009.199 enzyme inhibition rate and an immunochromatography detection method of various markers (colloidal gold, fluorescent microspheres and quantum dot microspheres) which are developed in recent years. The pretreatment of the two methods is relatively simple, in a laboratory, the peel of fruits and vegetables or She Jiansui is taken to be 1cm square, a certain amount of the fruit and vegetable is weighed and put into an extraction tank, a specific extracting solution is added, after oscillation extraction, a certain amount of liquid to be detected is manually absorbed in a test tube to react with various reagents under different time and temperature conditions, and finally the test result is obtained by measuring and reading the value on equipment. The prior method for quickly detecting pesticide residues has the disadvantages that the dependence of pretreatment and detection processes on operators is still too heavy, the strict standardization of process control is difficult to realize, and the stability and reliability of results are not high.
Disclosure of Invention
The invention aims to solve the technical problem that aiming at the defects in the prior art, the invention provides the detection box and the detection method which are simple, convenient, stable, reliable, high in efficiency, independent of laboratories and professional experimenters, standardized and capable of realizing automatic detection, and meets the requirements of the agricultural production and food safety field on rapid and accurate detection of pesticide residues.
The technical scheme adopted by the invention for solving the technical problems is as follows: the integrated pesticide residue standardized rapid detection box comprises a detection box body, wherein a sampling cabin, a sample detection cabin and a comparison cabin are arranged in the detection box body; a drainage pipeline is arranged in the sampling cabin; a liquid inlet of the drainage pipeline extends downwards into the liquid in the sampling cabin, and a liquid outlet extends upwards to be communicated with the upper end of the sample detection cabin; the top of the sample detection cabin is provided with a connecting interface for connecting a negative pressure generator; the negative pressure generator drives the liquid in the sampling cabin to flow into the sample detection cabin along the drainage pipeline through the connecting interface; the detection box body is also internally provided with a first reagent adding component for adding a reagent into the sample detection cabin and a second reagent adding component for adding the same reagent into the contrast cabin; the outer side surface of the sample detection cabin and the outer side surface of the comparison cabin are both provided with light-transmitting light detection windows;
the integrated pesticide residue standardized rapid detection box comprises a first reagent adding assembly, a second reagent adding assembly and a detection module, wherein the first reagent adding assembly comprises a plurality of mutually independent first reagent tubes which are arranged in a sample detection cabin; the top of the first reagent tube is provided with a first protective cover, and the bottom of the first reagent tube is provided with a first drainage valve for preventing the reagent in the first reagent tube from flowing out; the first liquid discharge valve can be opened by external force to enable the reagent in the first reagent tube to flow into the sample detection cabin;
the integrated pesticide residue standardized rapid detection box comprises a first reagent adding assembly, a second reagent adding assembly, a control cabin and a detection module, wherein the first reagent adding assembly comprises a plurality of first reagent tubes which are arranged in the control cabin and are independent from each other; a second protective cover is arranged at the top of the second reagent tube, and a second liquid discharge valve for preventing the reagent in the second reagent tube from flowing out is arranged at the bottom of the second reagent tube; the second liquid discharge valve can be opened by external force to enable the reagent in the second reagent tube to flow into the control cabin;
the integrated pesticide residue standardized rapid detection box is characterized in that the liquid outlet of the drainage pipeline is positioned above the liquid level of the mixed liquid in the sample detection cabin;
the integrated pesticide residue standardized rapid detection box is characterized in that the detection box is also provided with sealing films for sealing the sampling cabin, the connecting interface, the first reagent adding component and the second reagent adding component; the sealing film is tightly attached to the outer edge of the detection box body through hot pressing or gluing;
the integrated pesticide residue standardized rapid detection box is characterized in that two optical detection windows are positioned below a detection box body;
the invention also discloses a detection method for the integrated standardized rapid detection of pesticide residue, wherein the detection box comprises a detection box body, a sampling cabin, a sample detection cabin and a comparison cabin are arranged in the detection box body, and buffer solutions without pesticide are arranged in the sampling cabin and the comparison cabin, wherein the buffer solution in the sampling cabin is an extracting solution, and the buffer solution in the comparison cabin is a comparison solution; the detection method comprises the following steps:
the invention relates to a detection method for integrated pesticide residue standardized rapid detection, wherein,
s10: adding a sample to be detected into the sampling cabin, and fully soaking the sample to be detected in the extracting solution in the sampling cabin to obtain a detection sample liquid of the sample to be detected;
s20: driving the detection sample liquid in the S10 to flow into the sample detection cabin along a drainage pipeline by using a power device;
s30: adding a reagent in a first reagent adding assembly and the same reagent in a second reagent adding assembly into the detection sample liquid in the sample detection chamber and the contrast liquid in the contrast chamber respectively to obtain a to-be-detected liquid and a standard liquid;
s40: applying a heat source not exceeding 110 ℃ to the bottom of the detection box, and simultaneously incubating the solution to be detected and the standard solution;
s50: simultaneously carrying out spectrophotometry detection on the liquid to be detected and the standard liquid, comparing the light detection result of the liquid to be detected with the light detection result of the standard liquid, and if the light detection result of the liquid to be detected is different from the light detection result of the standard liquid, determining that the liquid to be detected contains pesticide;
the invention relates to a detection method for integrated standardized rapid detection of pesticide residue, wherein the outer side surface of a sample detection cabin and the outer side surface of a comparison cabin are both provided with light-transmitting light detection windows; external light respectively performs spectrophotometric detection on the liquid to be detected and the standard liquid through the two light detection windows;
the invention relates to a detection method for integrated pesticide residue standardized rapid detection, wherein in S20, a power device is a negative pressure generator connected with a connecting interface at the top of a sample detection cabin; enabling a negative pressure generator to generate a negative pressure in a sample detection chamber, and enabling the detection sample liquid in the sampling chamber to automatically flow into the sample detection chamber;
the invention relates to a detection method for integrated pesticide residue standardized rapid detection, wherein buffer solutions in a sampling cabin and a control cabin are distilled water.
The invention has the beneficial effects that: the integrated pesticide residue standardized rapid detection box and the detection method have ingenious design, and a sampling cabin, a sample detection cabin, a comparison cabin, a first reagent adding component and a second reagent adding component are arranged in the detection box; buffer solutions are preset in the sampling cabin and the comparison cabin, when in detection, a sample is placed in the sampling cabin to be fully soaked in the sampling cabin, a negative pressure generator is started to enable the sample detection cabin to be in negative pressure, liquid with the sample automatically flows into the sample detection cabin from the sampling cabin along a drainage pipeline, then a first reagent assembly and a second reagent assembly are started to respectively add the same reagent into the sample detection cabin and the comparison cabin, and after oscillation and mixing, spectrophotometric detection is respectively carried out on the liquid in the sample detection cabin and the liquid in the comparison cabin through light; the detection kit can complete all detection operations of sample addition, sample extraction, sample transfer, reagent addition, uniform mixing, incubation reaction and detection contrast in the detection kit only by a few simple external operations, and can complete detection without professional operators and professional experimental environments; and the detection process is located in the detection box, the detection environment is stable, the influence of human factors on the detection process is reduced, the stability of the detection process is ensured, a sample detection cabin and a comparison cabin are arranged in the detection box for comparison, whether pesticide residues exist in the sample detection cabin can be detected quickly during optical detection, and the detection efficiency is high.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the present invention will be further described with reference to the accompanying drawings and embodiments, wherein the drawings in the following description are only part of the embodiments of the present invention, and for those skilled in the art, other drawings can be obtained without inventive efforts according to the accompanying drawings:
FIG. 1 is a schematic structural diagram of an integrated standardized rapid pesticide residue detection kit according to a preferred embodiment of the present invention;
FIG. 2 is a schematic diagram of the structure of the sample extraction state of the integrated standardized rapid pesticide residue detection kit according to the preferred embodiment of the present invention;
FIG. 3 is a schematic diagram of a sample injection state of the integrated standardized rapid pesticide residue detection cartridge according to the preferred embodiment of the present invention;
FIG. 4 is a schematic diagram of the structure of the reagent added to the integrated standardized rapid pesticide residue detection kit according to the preferred embodiment of the present invention.
Detailed Description
The terms "first," "second," "third," and "fourth," etc. in the description and claims of the invention and in the accompanying drawings are used for distinguishing between different objects and not for describing a particular order. Furthermore, the terms "include" and "have," as well as any variations thereof, are intended to cover non-exclusive inclusions. For example, a process, method, system, article, or apparatus that comprises a list of steps or elements is not limited to only those steps or elements listed, but may alternatively include other steps or elements not listed, or inherent to such process, method, article, or apparatus.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the invention. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. It is explicitly and implicitly understood by one skilled in the art that the embodiments described herein can be combined with other embodiments.
"plurality" means two or more. "and/or" describes the association relationship of the associated objects, meaning that there may be three relationships, e.g., a and/or B, which may mean: a exists alone, A and B exist simultaneously, and B exists alone. The character "/" generally indicates that the former and latter associated objects are in an "or" relationship.
Also, the terms "upper, lower, front, rear, left, right, upper, lower, longitudinal" and the like, which denote orientation, are used with reference to the attitude and position of the device or apparatus in the present disclosure during normal use.
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without inventive step, are within the scope of the present invention.
The first embodiment is as follows:
the integrated pesticide residue standardized rapid detection box disclosed by the preferred embodiment of the invention is shown in figures 1-4 and comprises a detection box body 100, wherein the detection box body 100 is internally provided with a sampling cabin 101, a sample detection cabin 102 and a comparison cabin 103; it is worth to be noted that the sampling chamber is provided with an opening for the sample 10 to be tested to enter, and the sampling chamber 101 and the comparison chamber 103 are both provided with a buffer solution without pesticide, wherein the buffer solution in the sampling chamber 101 is an extracting solution 108, the buffer solution in the comparison solution 109 is a comparison solution 109, and preferably, the buffer solution is distilled water; a drainage pipeline 104 is arranged in the sampling cabin 101; a liquid inlet of the drainage pipeline 104 extends downwards into the liquid in the sampling cabin 101, and a liquid outlet extends upwards to be communicated with the upper end of the sample detection cabin 102; it is worth to explain that the liquid inlet end of the drainage pipeline is positioned at the bottom of the sampling cabin; the top of the sample detection chamber 102 is provided with a connection interface 105 for connecting a negative pressure generator (not shown); a negative pressure generator (not shown) drives the liquid in the sampling chamber 101 to flow into the sample detection chamber 102 along the drainage pipeline 104 through the connection interface 105; it is worth to be noted that, the negative pressure generator is the prior art, and the negative pressure generator can be replaced by an air pump and is used for generating negative pressure in the sample detection cabin, so that the liquid in the sampling cabin automatically flows into the sample detection cabin; the detection box body 100 is also internally provided with a first reagent adding component 200 for adding a reagent into the sample detection chamber 102 and a second reagent adding component 300 for adding the same reagent into the control chamber 103; the reagent in the first reagent adding assembly is the same as the reagent in the second reagent adding assembly; the outer side surface of the sample detection cabin and the outer side surface of the comparison cabin 103 are both provided with light-transmitting light detection windows 106; during detection, light rays simultaneously penetrate through the two optical detection windows to simultaneously perform spectrophotometric detection on the to-be-detected liquid 110 and the standard liquid 111, the photodetection result of the to-be-detected liquid 110 is compared with the photodetection result of the standard liquid 111, if the photodetection result of the to-be-detected liquid 110 is different from the photodetection result of the standard liquid 111, the to-be-detected liquid 110 contains pesticide, and the operation is simple.
The integrated pesticide residue standardized rapid detection box is ingenious in design, the detection process is standardized according to the GB/T5009.199 standard, and a sampling cabin, a sample detection cabin, a comparison cabin, a first reagent adding component and a second reagent adding component are arranged in the detection box; buffer solutions are preset in the sampling cabin and the comparison cabin, during detection, a sample to be detected is added into the sampling cabin, the sample to be detected is fully soaked in an extracting solution in the sampling cabin to obtain a detection sample liquid of the sample to be detected, a negative pressure generator is started to enable the sample detection cabin to be in a negative pressure state, the detection sample liquid in the sampling cabin automatically flows into the sample detection cabin from the sampling cabin along a drainage pipeline, then a first reagent assembly and a second reagent assembly are started to respectively add the same reagents into the sample detection cabin and the comparison cabin, after oscillation and mixing, spectrophotometric detection is respectively carried out on the liquid in the sample detection cabin and the liquid in the comparison cabin through light; the detection box can complete all detection operations of sample addition, sample extraction, sample transfer, reagent addition, uniform mixing, incubation reaction and detection contrast in the detection box only by a few simple external operations, and can complete detection without professional operators and professional experimental environments; and the detection process is located in the detection box, the detection environment is stable, the influence of human factors on the detection process is reduced, the stability of the detection process is ensured, the light detection result in the sample detection cabin in the detection box is compared with the light detection result in the comparison cabin, whether pesticide residues exist in the sample detection cabin can be detected quickly, and the detection efficiency is high.
Preferably, the first reagent adding assembly 200 comprises a plurality of mutually independent first reagent tubes 201 arranged in the sample detection chamber 102; the top of the first reagent tube 201 is provided with a first protective cover 202, and the bottom is provided with a first drainage valve 203 for preventing the reagent in the first reagent tube 201 from flowing out; the first drain valve 203 can be opened by an external force to allow the reagent in the first reagent tube 201 to flow into the sample detection chamber 102. Preferably, the first reagent assembly is located at the top of the sample detection chamber; when adding reagent toward the sample test under-deck, only need open first protective cover, rethread stirring rod or other pressure poles push away the intraductal first drainage valve of arbitrary first reagent downwards, make first drainage valve lapse, the intraductal reagent of first reagent flows into the sample test under self gravity in the under-deck, easy operation, safe and reliable.
Preferably, the second reagent adding assembly 300 comprises a plurality of second reagent tubes 301 which are independent from each other and are arranged in the control chamber 103; a second protective cover 302 is arranged at the top of the second reagent tube 301, and a second drain valve 303 for preventing the reagent in the second reagent tube 301 from flowing out is arranged at the bottom; the second drain valve 303 can be opened by an external force to allow the reagent in the second reagent tube 301 to flow into the control chamber 103; the second reagent adding assembly is positioned at the top of the sample detection cabin; the structure of the second reagent adding assembly can be the same as that of the first reagent adding assembly, the action principle is also the same, and it is worth to be noted that, when reagent is added each time, the first reagent adding assembly and the second reagent adding assembly are the same reagent added, and the amount of the reagent added each time is also the same, so that variable factors are reduced.
It is worth mentioning that the first drain valve and the second drain valve can be controllably opened under the action of pressure, mechanical thrust or electromagnetic force.
Adding the reagent in the first reagent adding component 200 and the same reagent in the second reagent adding component 300 into the detection sample liquid in the sample detection chamber 102 and the contrast liquid 109 in the contrast chamber 103 respectively to obtain a to-be-detected liquid 110 and a standard liquid 111; simultaneously carrying out spectrophotometric detection on the liquid to be detected 110 and the standard liquid 111, comparing the light detection result of the liquid to be detected 110 with the light detection result of the standard liquid 111, and if the light detection result of the liquid to be detected 110 is different from the light detection result of the standard liquid 111, determining that the liquid to be detected 110 contains pesticide;
preferably, before the spectrophotometric detection, a heat source not higher than 110 ℃ can be applied to the bottom of the detection box, and the solution to be detected and the standard solution are incubated to accelerate the reaction.
Preferably, the liquid outlet of the drainage tube 104 is located above the liquid level of the mixed liquid in the sample detection chamber 102, so that the liquid in the extraction chamber can flow into the sample detection chamber in a single direction, and the liquid in the extraction chamber can flow into the sample detection chamber smoothly.
Preferably, the detection box is further provided with a sealing film 107 for sealing the sampling chamber 101, the connection interface 105, the first reagent adding assembly 200 and the second reagent adding assembly 300; the sealing film 107 is tightly attached to the outer edge of the detection box body 100 by hot pressing or gluing; because the top in sample cabin is equipped with the opening, sample test cabin accessible connection interface and external connection, it is sealed with sample cabin, connection interface, first reagent interpolation subassembly and second reagent interpolation subassembly through the seal membrane, can guarantee that the detection box is in encapsulated situation all the time when not using, can effectively prevent in impurity such as external gas, dust gets into the detection box, influences the internal environment, and the testing result is inaccurate when leading to detecting.
Preferably, the sealing film 107 is also provided with an easy-to-tear opening, so that the sealing film can be opened without using an external tool during use, and the operation is convenient.
Preferably, the two light inspection windows 106 are located below the main body 100 of the detection box, so that the user can directly observe the light inspection result in the sample detection chamber and the light inspection structure in the comparison chamber.
Example two:
the invention discloses a detection method for detecting organophosphorus and carbamate pesticides in Chinese cabbage, which is applied to a detection box in the first embodiment and comprises the following steps:
the detection box is pre-filled with 5 ml of buffer solution for extraction in the extraction cabin, 2ml of buffer solution for comparison in the comparison cabin, the detection cabin is empty, and three reagent tubes in the first reagent adding assembly and the second reagent adding assembly above the sample detection cabin and the comparison cabin are pre-filled with three reagents for detecting pesticide residues used for testing, namely an enzyme reagent, a color developing agent and a substrate respectively.
Sample treatment: sampling Chinese cabbage according to a standard to obtain a sample for detection, transferring the sample to an extraction cabin of a detection box, and soaking the sample to be detected in a buffer solution; and horizontally shaking the detection box for 1 minute to fully soak the sample to be detected in the buffer solution to obtain the detection sample liquid of the sample to be detected.
Sample introduction: and applying negative pressure to the connection interface at the upper part of the sample detection chamber, wherein the negative pressure is formed in the sample detection chamber, and the air pressure in the extraction chamber is greater than that in the sample detection chamber, so that the detection sample liquid in the extraction chamber flows into the sample detection chamber along the drainage pipeline.
Adding a reagent: opening first liquid discharge valves of two reagent pipes of the enzyme reagent and the color developing agent in the first reagent adding assembly, adding the enzyme reagent and the color developing agent into the sample detection cabin to be mixed with the detection sample liquid, simultaneously opening second liquid discharge valves of two reagent pipes of the enzyme reagent and the color developing agent in the second reagent adding assembly, and adding the enzyme reagent and the color developing agent into the control cabin to be mixed with the control liquid; the cartridge was shaken horizontally for 30 seconds while incubating for 10 minutes. After the incubation is finished, a first liquid discharge valve of a substrate reagent tube in the first reagent adding assembly is opened, the substrate reagent is added into the sample detection cabin to obtain the liquid to be detected, meanwhile, a second liquid discharge valve of the substrate reagent tube in the second reagent adding assembly is opened, and the substrate reagent is added into the control cabin to obtain the standard liquid.
Sample detection: and after the horizontal shaking detection box is uniformly mixed for 30 seconds, performing countdown photometric change detection on a photometric window of the sample detection cabin and a photometric window of the comparison cabin for 3 minutes by light.
Control reference: the light detection result of the comparison cabin provides reference comparison for the light detection result of the sample detection cabin, and the enzyme inhibition rate of the sample to be detected can be obtained through calculation so as to determine whether pesticide residues exist in the sample to be detected.
The principle is as follows: under certain conditions, organophosphorus and carbamate pesticides have an inhibiting effect on the normal function of cholinesterase, and the inhibition rate is positively correlated with the concentration of the pesticides. Normally, enzyme catalyzes the hydrolysis of nerve conduction metabolites (acetylcholine), the hydrolysis product reacts with a color developing agent to generate a yellow substance, a spectrophotometer is used for measuring the change value of absorbance along with time at 412nm, the inhibition rate is calculated, and whether organophosphorus or carbamate pesticides exist in a sample can be judged through the inhibition rate.
The process of "measuring the change in absorbance with time at 412nm with a spectrophotometer" in the above example is generally referred to as "photodetection" operation in the process of measuring the pesticide residue.
Example three:
the invention discloses a detection method for detecting sulfur dioxide residue in tremella, which is applied to a detection box in the first embodiment and comprises the following steps:
9.5 ml of extraction buffer solution is preloaded in the extraction cabin of the detection box, 2ml of comparison buffer solution is preloaded in the comparison cabin, the sample detection cabin is empty, and two reagent tubes of a first reagent adding assembly and a second reagent adding assembly above the sample detection cabin and the comparison cabin are respectively preloaded with two reagents for sulfur dioxide rapid detection used for testing: reagent A and reagent B.
Sample treatment: 0.5g of dried tremella sample is weighed according to the standard, and then transferred to an extraction cabin of a sample detection box, so that the sample to be detected is soaked in a buffer solution in the extraction cabin. And horizontally shaking the detection box for 5 minutes to fully soak the sample to be detected in the buffer solution to obtain the detection sample liquid of the sample to be detected.
Sample introduction: and applying negative pressure to the connection interface at the upper part of the sample detection chamber, wherein the negative pressure is formed in the sample detection chamber, the air pressure in the extraction chamber is greater than the air pressure in the sample detection chamber, and the detection sample liquid in the extraction chamber quantitatively flows into the sample detection chamber along the drainage pipeline by 2ml under the action of atmospheric pressure.
Adding a reagent: opening a first liquid discharge valve of a reagent A reagent pipe in the first reagent adding assembly, adding the reagent A into the sample detection cabin to be mixed with the detection sample liquid, and simultaneously opening a second liquid discharge valve of a reagent A reagent pipe in the second reagent adding assembly, adding the reagent A into the contrast cabin to be mixed with the contrast liquid; the cartridge was shaken horizontally for 30 seconds while incubating for 10 minutes. After the incubation is finished, opening a first liquid discharge valve of a reagent B reagent tube in the first reagent adding assembly, adding the reagent B into the sample detection cabin to obtain a liquid to be detected, simultaneously opening a second liquid discharge valve of a reagent B reagent tube in the second reagent adding assembly, and adding the reagent B into the control cabin to obtain a standard liquid; shake and mix for 30 seconds.
Sample detection: and after standing for 10 minutes, carrying out absorbance detection on the light detection window of the sample detection cabin and the light detection window of the comparison cabin through light rays.
Control reference: the light detection result of the comparison cabin provides reference comparison for the light detection result of the sample detection cabin, and the sulfur dioxide concentration of the detected sample can be obtained through calculation so as to determine whether sulfur dioxide residue exists in the sample to be detected.
The principle is as follows: the method comprises the steps of reacting sulfite with sodium tetrachloromercuric oxide to generate a stable complex, reacting with formaldehyde and pararosaniline hydrochloride to generate a mauve complex, measuring absorbance at 550nm by using a spectrophotometer, and quantifying the concentration of sulfur dioxide by adopting a standard curve method.
The hydrochloric pararosaniline ultraviolet spectrophotometric method is a classic sulfur dioxide determination method, and is listed as the 1 st method by the national food standard method of China. However, this method is not suitable for samples containing large amounts of volatile fatty acids or partially colored samples.
The procedure of "measuring the absorbance at 550nm with a spectrophotometer" in the above example is generally referred to as a "photometric" operation of the procedure of measuring the sulfur dioxide residue.
It will be understood that modifications and variations can be made by persons skilled in the art in light of the above teachings and all such modifications and variations are intended to be included within the scope of the invention as defined in the appended claims.
Claims (10)
1. The integrated pesticide residue standardized rapid detection box comprises a detection box body, and is characterized in that a sampling cabin, a sample detection cabin and a comparison cabin are arranged in the detection box body; a drainage pipeline is arranged in the sampling cabin; the liquid inlet of the drainage pipeline extends downwards into the liquid in the sampling cabin, and the liquid outlet extends upwards to be communicated with the upper end of the sample detection cabin; the top of the sample detection cabin is provided with a connecting interface for connecting a negative pressure generator; the negative pressure generator drives the liquid in the sampling cabin to flow into the sample detection cabin along the drainage pipeline through the connecting interface; the detection box body is also internally provided with a first reagent adding component for adding a reagent into the sample detection cabin and a second reagent adding component for adding the same reagent into the contrast cabin; and the outer side surface of the sample detection cabin and the outer side surface of the comparison cabin are both provided with light-transmitting light detection windows.
2. The cartridge of claim 1, wherein the first reagent adding assembly includes a plurality of independent first reagent tubes disposed in the sample detection compartment; the top of the first reagent tube is provided with a first protective cover, and the bottom of the first reagent tube is provided with a first drainage valve for preventing the reagent in the first reagent tube from flowing out; the first drainage valve can be opened by an external force to enable the reagent in the first reagent tube to flow into the sample detection cabin.
3. The cartridge according to claim 1 or 2, wherein the second reagent adding member includes a plurality of second reagent tubes provided in the control chamber independently of each other; the top of the second reagent tube is provided with a second protective cover, and the bottom of the second reagent tube is provided with a second liquid discharge valve for preventing the reagent in the second reagent tube from flowing out; the second drain valve can be opened by external force to make the reagent in the second reagent tube flow into the control cabin.
4. The testing cassette of claim 3, wherein the outlet of the drainage tube is positioned above the level of the mixed liquid in the sample testing compartment.
5. The testing cassette of claim 4, wherein said testing cassette further comprises sealing films for sealing said sampling compartment, said connection interface, said first reagent adding assembly and said second reagent adding assembly; the sealing membrane is closely attached to the outer edge of the detection box body through hot pressing or gluing.
6. The cartridge of claim 5, wherein both of the light inspection windows are located below the cartridge body.
7. An integrated pesticide residue standardized rapid detection method, which is characterized in that the integrated pesticide residue standardized rapid detection method is applied to the detection box of any one of claims 1 to 6, and comprises a detection box body, wherein a sampling cabin, a sample detection cabin and a control cabin are arranged in the detection box body, and buffer solutions without pesticides are arranged in the sampling cabin and the control cabin, wherein the buffer solution in the sampling cabin is an extracting solution, and the buffer solution in the control cabin is a control solution; the detection method comprises the following steps:
s10: adding a sample to be detected into the sampling cabin, and fully soaking the sample to be detected in the extracting solution in the sampling cabin to obtain a detection sample liquid of the sample to be detected;
s20: driving the detection sample liquid in the S10 to flow into the sample detection cabin along a drainage pipeline by using a power device;
s30: adding a reagent in a first reagent adding assembly and the same reagent in a second reagent adding assembly into the detection sample liquid in the sample detection chamber and the contrast liquid in the contrast chamber respectively to obtain a to-be-detected liquid and a standard liquid;
s40: applying a heat source not exceeding 110 ℃ to the bottom of the detection box, and simultaneously incubating the solution to be detected and the standard solution;
s50: and simultaneously carrying out spectrophotometry detection on the liquid to be detected and the standard liquid, comparing the light detection result of the liquid to be detected with the light detection result of the standard liquid, and if the light detection result of the liquid to be detected is different from the light detection result of the standard liquid, determining that the liquid to be detected contains pesticide.
8. The integrated pesticide residue standardized rapid detection method as claimed in claim 7, wherein the outer side surface of the sample detection chamber and the outer side surface of the control chamber are both provided with light-transmitting light detection windows; and external light respectively performs spectrophotometric detection on the liquid to be detected and the standard liquid through the two light detection windows.
9. The method for detecting the standardized and rapid detection of the pesticide residue as claimed in the claim 8, wherein in the step S20, the power device is a negative pressure generator connected with a connection interface at the top of the sample detection cabin; and generating negative pressure in the sample detection chamber through the negative pressure generator, and automatically flowing the detection sample liquid in the sampling chamber into the sample detection chamber.
10. The method for detecting the standardized and rapid detection of the pesticide residue as claimed in claim 9, wherein the buffer solution in the sampling chamber and the buffer solution in the control chamber are distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211569190.XA CN115656531B (en) | 2022-12-08 | 2022-12-08 | Integrated pesticide residue standardized rapid detection box and detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211569190.XA CN115656531B (en) | 2022-12-08 | 2022-12-08 | Integrated pesticide residue standardized rapid detection box and detection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115656531A true CN115656531A (en) | 2023-01-31 |
| CN115656531B CN115656531B (en) | 2023-03-31 |
Family
ID=85019727
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211569190.XA Active CN115656531B (en) | 2022-12-08 | 2022-12-08 | Integrated pesticide residue standardized rapid detection box and detection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115656531B (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102735563A (en) * | 2011-04-12 | 2012-10-17 | 精工爱普生株式会社 | Detection device |
| US20140093975A1 (en) * | 2011-03-25 | 2014-04-03 | Shenzhen Mindray Bio-Medical Electronics Co., Ltd. | Automatic analyzer and sample analysis method |
| CN104793004A (en) * | 2015-04-24 | 2015-07-22 | 杭州霆科生物科技有限公司 | Pesticide residue detection micro-fluidic chip with pre-stored reaction reagent |
| CN109001195A (en) * | 2018-09-13 | 2018-12-14 | 常州常检诺食品检测中心有限公司 | A kind of vegetable pesticide residue detection method |
| CN209803109U (en) * | 2019-01-21 | 2019-12-17 | 海南出入境检验检疫局检验检疫技术中心 | Portable pesticide residue short-term test appearance |
| CN210604395U (en) * | 2019-07-26 | 2020-05-22 | 中华人民共和国阿克苏海关 | Multi-component pesticide residue detection device in agricultural products |
| CN111272748A (en) * | 2020-03-09 | 2020-06-12 | 佛山市铬维科技有限公司 | Pesticide residue detection device |
| CN213986206U (en) * | 2020-12-04 | 2021-08-17 | 广州市来利洪饼业有限公司 | Wheat flour pesticide residue rapid detection equipment |
| CN215525546U (en) * | 2021-08-23 | 2022-01-14 | 湖北久华食安生物技术有限公司 | Injection type pesticide residue rapid detection kit |
-
2022
- 2022-12-08 CN CN202211569190.XA patent/CN115656531B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140093975A1 (en) * | 2011-03-25 | 2014-04-03 | Shenzhen Mindray Bio-Medical Electronics Co., Ltd. | Automatic analyzer and sample analysis method |
| CN102735563A (en) * | 2011-04-12 | 2012-10-17 | 精工爱普生株式会社 | Detection device |
| CN104793004A (en) * | 2015-04-24 | 2015-07-22 | 杭州霆科生物科技有限公司 | Pesticide residue detection micro-fluidic chip with pre-stored reaction reagent |
| CN109001195A (en) * | 2018-09-13 | 2018-12-14 | 常州常检诺食品检测中心有限公司 | A kind of vegetable pesticide residue detection method |
| CN209803109U (en) * | 2019-01-21 | 2019-12-17 | 海南出入境检验检疫局检验检疫技术中心 | Portable pesticide residue short-term test appearance |
| CN210604395U (en) * | 2019-07-26 | 2020-05-22 | 中华人民共和国阿克苏海关 | Multi-component pesticide residue detection device in agricultural products |
| CN111272748A (en) * | 2020-03-09 | 2020-06-12 | 佛山市铬维科技有限公司 | Pesticide residue detection device |
| CN213986206U (en) * | 2020-12-04 | 2021-08-17 | 广州市来利洪饼业有限公司 | Wheat flour pesticide residue rapid detection equipment |
| CN215525546U (en) * | 2021-08-23 | 2022-01-14 | 湖北久华食安生物技术有限公司 | Injection type pesticide residue rapid detection kit |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115656531B (en) | 2023-03-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108375559A (en) | Cardiac troponin kit and preparation based on micro-fluidic chip and detection method | |
| JP3051649B2 (en) | Apparatus and method for non-contamination treatment of reaction chain | |
| KR100562179B1 (en) | Analyte concentration measurement method using hollow frustum | |
| KR100562178B1 (en) | Hollow frustum reagent test device | |
| CN206696174U (en) | A kind of high flux garden stuff pesticide residue fast detector | |
| JP2015515632A (en) | Biochemical assay cartridge | |
| EP0753583A4 (en) | Method of quantitative analysis of cholesterol | |
| Tiffany et al. | Clinical evaluation of kinetic enzymatic fixed-time and integral analysis of serum triglycerides | |
| CN110218769B (en) | Integrated multi-linked test strip for LAMP or RT-LAMP | |
| CN204666516U (en) | A kind of Detecting Pesticide micro-fluidic chip of pre-stored reaction reagent | |
| CN115656531B (en) | Integrated pesticide residue standardized rapid detection box and detection method | |
| ATE231612T1 (en) | FAST RESPONSIVE TEST DEVICE | |
| Kricka et al. | [33] Photographic detection of chemiluminescent and bioluminescent reactions | |
| CN109917123A (en) | A kind of residual detection device of agriculture based on DELFIA and detection method | |
| CN108548931A (en) | Free thyroxine fT4 kits and preparation and detection method are detected based on micro-fluidic chip | |
| US5063024A (en) | Method and apparatus for immunological determinations | |
| CN218381981U (en) | Sample pretreatment device for portable methanol rapid detection | |
| CN111537723A (en) | Test strip and method for detecting chicken doped in beef and mutton | |
| CN215678418U (en) | Immunochromatographic detection device | |
| CN110426366A (en) | Meat adulteration ratio detection method based on visualization olfactory sensor and near-infrared | |
| CN212964985U (en) | Immunochromatographic detection device | |
| US4319884A (en) | Automatic colorimetric analyzer | |
| JPH08313535A (en) | Reagent container | |
| CN210136235U (en) | A whole blood sample detection device | |
| CN210199120U (en) | Pesticide residue detection chip integrating enzyme inhibition method and immune array |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |