CN115637266A - Reagent extraction box for nucleic acid detection system and use method thereof - Google Patents
Reagent extraction box for nucleic acid detection system and use method thereof Download PDFInfo
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- CN115637266A CN115637266A CN202211240655.7A CN202211240655A CN115637266A CN 115637266 A CN115637266 A CN 115637266A CN 202211240655 A CN202211240655 A CN 202211240655A CN 115637266 A CN115637266 A CN 115637266A
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Abstract
The invention discloses a reagent extraction box for a nucleic acid detection system and a use method thereof, wherein the reagent extraction box comprises: the storage fixing plate is provided with a plurality of storage holes, a plurality of storage tubes are sealed at the lower part and opened at the top, and each storage tube is coaxially arranged with the corresponding storage hole respectively, wherein the number of the storage tubes is at least 7, and the storage tubes are respectively a sample tube for containing a sample therein, a cracking storage tube for storing cracking liquid therein, a medium storage tube for storing a target capture medium solution therein, a washing storage tube for storing washing liquid therein, an elution storage tube for storing eluent therein, a stirring storage tube for placing a stirring sleeve therein, and a gun head tube for storing a liquid-transfering gun head therein; the invention can realize the purpose of nucleic acid extraction and detection, does not need personnel to intervene in the whole process, really achieves the purposes of adding a sample by a laboratory technician, automatically finishes the extraction and detection by an instrument, is connected seamlessly in the whole detection process, and can shorten the detection time to 0.5 to 1 hour.
Description
Technical Field
The invention belongs to the technical field of molecular diagnosis of medical instruments, and particularly relates to a reagent extraction kit for a nucleic acid detection system and a use method thereof.
Background
In the technical field of molecular diagnosis, nucleic acid detection is divided into two parts, wherein the first part is nucleic acid extraction and needs to be extracted in a matched nucleic acid extraction kit. The second part is nucleic acid amplification detection, and the extracted product needs to be transferred into an amplification tube through a manual liquid transfer device or an automatic liquid transfer device for nucleic acid amplification and detection.
The existing nucleic acid detection operation process is complex, the requirement on personnel is high, the extraction of nucleic acid to be detected is firstly completed, a sample to be detected is firstly added into a nucleic acid extraction kit manually, then the kit added with the sample is put into nucleic acid extraction equipment for nucleic acid extraction, after the extraction is completed, personnel are required to transfer a nucleic acid extract to a detection tube manually, then nucleic acid amplification detection is performed, before the nucleic acid amplification, a reagent needs to be added into a nucleic acid amplification tube (PCR tube) manually, and then the extracted nucleic acid is transferred into the nucleic acid amplification tube added with the reagent manually. And placing the nucleic acid amplification tube added with the reagent and the sample in a real-time fluorescent quantitative PCR instrument for nucleic acid amplification and detection. The whole detection process is tedious in operation, needs manual intervention, performs manual pipetting for 3 times, and respectively loads a nucleic acid kit, transfers a nucleic acid extract to a PCR tube, and loads a reagent to the PCR tube. 2 pieces of equipment, nucleic acid extractor and PCR instrument, were operated. And 2 times of different reagent consumables are loaded, namely the nucleic acid extraction kit and the PCR amplification tube.
The more the number of intervention times of the existing nucleic acid detecting personnel is, the more errors are easy to occur, and the extraction or detection result is abnormal. The whole detection process has certain requirements on the experimental environment and needs to be carried out in a laboratory, and the preparation process, the nucleic acid extraction process, the nucleic acid amplification process and the detection process of the sample need to be completed in independent spaces, so that cross contamination is prevented. The same person needs to enter and exit different independent spaces along with the detection progress, or each independent space is provided with a respective experimenter. The whole detection process, from the preparation of the sample, to the extraction of the nucleic acid, to the amplification and detection of the nucleic acid, is relatively long, and is expected to take 2-4 hours.
Disclosure of Invention
The invention aims to provide a reagent extraction box for a nucleic acid detection system and a using method thereof, and aims to solve the problems that the prior art has high requirements on laboratory sites, the workload of workers is busy, and the detection result duration is too long.
The invention adopts the following technical scheme: a reagent extraction cartridge for a nucleic acid detection system, comprising:
a storage fixing plate horizontally arranged and provided with a plurality of storage holes,
a plurality of storage tubes which are positioned at the lower side of the storage fixing plate, the lower parts of the storage tubes are closed, the top parts of the storage tubes are opened, each storage tube is respectively arranged coaxially with the corresponding storage hole, the upper end opening of each storage tube is equal to the inner diameter of the corresponding storage hole and is integrally connected with the storage fixing plate and used for storing reagents required by each step of nucleic acid detection,
wherein, the memotron is provided with 7 at least, be used for the sample pipe that holds the sample in it respectively, the schizolysis memotron that has the lysate in it, the media memotron that has the target capture media solution in it, the washing memotron that has the washing liquid in it, the elution memotron that has the eluant in it, the stirring memotron that has placed the stirring cover in it, the rifle head pipe that has the liquid-transfering rifle head in it.
Furthermore, the washing storage tubes are provided with 2, and the two washing storage tubes respectively store a first washing liquid for removing protein and carbohydrate macromolecular substances and a second washing liquid for removing salts. .
Furthermore, the number of the washing storage pipes is 3, and the three washing storage pipes are respectively used for storing a first washing liquid for removing protein, a second washing liquid for removing saccharide macromolecular substances and a third washing liquid for removing salts.
Furthermore, the storage tube also comprises a detection tube for storing detection reagents, and the detection tube is used for eluting eluent containing nucleic acid in the storage tube and transferring the eluent into the detection tube to be detected.
Furthermore, the stirring sleeve is of a hollow tubular structure, the bottom of the stirring sleeve is closed, the top of the stirring sleeve is opened, an inner cavity of the stirring sleeve is used for enabling the magnetic rod to extend into the stirring sleeve from top to bottom, so that the target capture medium is adsorbed on the outer wall of the bottom of the stirring sleeve under the action of the magnetic rod, and the stirring sleeve is driven by a moving mechanism located at the upper part of the stirring sleeve to reciprocate up and down and stir the solution in which the stirring sleeve is located.
Further, the first section fixed connection of stirring cover has the earrings of level setting, the earrings are hollow annular structure, and the cover is established on the outer wall of stirring cover, the inner wall of earrings and the outer wall fixed connection of stirring cover, the earrings are used for the lower extreme of moving mechanism's manipulator to support the upside at the earrings, make the stirring cover the degree of depth of snatching at every turn the same, still be used for when the stirring cover is in the stirring storage tube, make the earrings take on the stirring storage tube, guarantee that the bottom of stirring cover does not touch the bottom of stirring storage tube, avoid the stirring cover to snatch the in-process by moving mechanism and produce the deformation.
Further, the storage tube still includes liquid sealing tube, and the intraductal storage of liquid sealing is used for carrying out the solution of sealing the pipe to each storage tube after extracting.
Further, the method also comprises the following steps: a supporting frame is arranged on the base plate,
the support frame includes:
a front side plate which is vertically arranged, the upper end of the front side plate is fixedly connected with the lower edge of the front side of the storage fixing plate,
a rear side plate which is vertically arranged, the upper end of the rear side plate is fixedly connected with the lower edge of the rear side of the storage fixing plate,
left side board and right side board, vertical setting, its upper end respectively with store the left side lower limb of fixed plate and right side lower limb fixed connection to mutually support with preceding curb plate and posterior lateral plate and form the framework, and then support storage fixed plate and each storage tube, when using, conveniently arrange the desktop in through the lower extreme of preceding curb plate, posterior lateral plate, left side board and right side board on, and guarantee that the level of storing the fixed plate is placed and the vertical of each storage tube is placed.
Furthermore, the lower ends of the front side plate and the rear side plate are provided with grooves from bottom to top, and the two grooves are used for grabbing and placing by testers.
A method of using a reagent extraction cartridge for a nucleic acid detection system, comprising a reagent extraction cartridge comprising:
a storage fixing plate horizontally arranged and provided with a plurality of storage holes,
a plurality of storage tubes which are positioned at the lower side of the storage fixing plate, the lower parts of the storage tubes are closed, the top parts of the storage tubes are opened, each storage tube is respectively arranged coaxially with the corresponding storage hole, the upper end opening of each storage tube is equal to the inner diameter of the corresponding storage hole and is integrally connected with the storage fixing plate and used for storing reagents required by each step of nucleic acid detection,
the storage tubes are at least 7, namely a sample tube for containing a sample, a cracking storage tube in which lysis solution is stored, a medium storage tube in which a target capture medium solution is stored, a washing storage tube in which washing solution is stored, an elution storage tube in which eluent is stored, a stirring storage tube in which a stirring sleeve is placed, and a gun head tube in which a liquid-transfering gun head is stored;
the using method comprises the following steps:
step S1: a manipulator is used for grabbing a liquid-transfer gun head in a gun head pipe, a preset amount of samples are sucked from the sample pipe, the sucked samples are placed into a cracking storage pipe,
step S2: after the existing pipette tip of the manipulator is discarded, a new pipette tip in the pipette tip tube is grabbed again, the solution of the target capture medium is sucked from the medium storage tube and is placed in the cracking storage tube,
and step S3: after the existing pipette head of the manipulator is discarded, the stirring sleeve in the stirring storage tube is grabbed, the stirring sleeve is driven to move up and down in the cracking storage tube to stir the sample so that the cells in the sample are cracked to obtain nucleic acid and adsorbed on the target capture medium, then the magnetic rod at the lower end of the manipulator is controlled to move downwards to stretch into the stirring sleeve so that the target capture medium is adsorbed on the outer wall at the lower end of the stirring sleeve under the action of the magnetic rod,
and step S4: the mechanical arm is used for moving the stirring sleeve absorbed with the target capture medium into the washing storage tube, the magnetic rod at the lower end of the mechanical arm is controlled to move upwards and withdraw from the inner cavity of the stirring sleeve, so that the target capture medium absorbed on the outer wall of the lower end of the stirring sleeve is released and enters the washing storage tube,
step S5: the stirring sleeve is controlled by a mechanical arm to move up and down in the washing storage pipe for stirring and washing, so that the washing liquid washes away proteins, saccharides and macromolecular substances and salts on the surface of the nucleic acid adsorbed by the target capture medium;
step S6: the magnetic rod and the stirring sleeve at the lower end of the manipulator are controlled to move the nucleic acid adsorbed by the target capture medium into the elution storage tube again, and the stirring sleeve moves up and down to stir and elute, so that the nucleic acid adsorbed on the target capture medium is eluted by the eluent,
step S7: and after the existing stirring sleeve of the manipulator is discarded, absorbing a new pipette tip in the pipette tip tube again, and absorbing eluent containing nucleic acid in the elution storage tube to place in the detection tube for detection.
The invention has the beneficial effects that: according to the invention, the nucleic acid extraction reagent, the PCR reaction solution, the pipette tip and the stirring sleeve are pre-packaged, the purpose of nucleic acid extraction and detection can be realized only by adding a sample by a client, personnel intervention is not required in the whole process, the sample is really added by a laboratory technician, and the extraction and detection purposes are automatically completed by an instrument; the invention realizes the transfer of the target capture medium to the PCR tube by the stirring sleeve and the magnetic rod, completes the amplification and detection of nucleic acid by the PCR tube, has seamless connection in the whole detection process, saves the process time of reagent preparation, sample addition and multiple consumable loading of an experimenter, can complete the detection without the insertion of different independent spaces by the same experimenter, and can shorten the detection time to 0.5 to 1 hour.
Drawings
FIG. 1 is a schematic view of the structure of the present invention;
FIG. 2 is a schematic view of the structure of the stirring sleeve of the present invention.
Wherein: 1. storing the fixed plate; 2. a storage tube; 3. a storage hole; 4. a support frame; 5. a front side plate; 6. a detection tube; 7. a gun head tube; 8. a right side plate; 9. a stirring sleeve 10 and an earring; 11. a sample tube; 12. a lysis storage tube; 13. a medium storage tube; 14. a stirring storage tube; 15. washing the storage tube; 16. and (4) eluting the storage tube.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.
The invention discloses a reagent extraction box for a nucleic acid detection system, which comprises a storage fixing plate 1 and a plurality of storage tubes 2 as shown in figures 1 and 2.
Store 1 level setting of fixed plate, a plurality of storage holes 3 have been seted up on the storage fixed plate 1, a plurality of storage tubes 2 are located the downside of storing fixed plate 1, the lower part of a plurality of storage tubes 2 is sealed, open-top, a plurality of storage tubes 2 respectively with the 3 coaxial settings in storage hole that correspond, the upper end opening of each storage tube 2 equals with the internal diameter in storage hole 3 that corresponds and with storage fixed plate 1 body coupling, a plurality of storage tubes 2 are used for storing the required reagent of each step of nucleic acid testing.
The storage tube 2 is at least provided with 7, and 7 storage tubes are respectively a sample tube 11 used for containing a sample therein, a cracking storage tube 12 in which a cracking solution is stored, a medium storage tube 13 in which a target capture medium solution is stored, a washing storage tube 15 in which a washing solution is stored, an elution storage tube 16 in which an eluent is stored, a stirring storage tube 14 in which a stirring sleeve 9 is placed, and a pipette tip tube 7 in which a pipette tip is stored. The storage tube 2 further comprises a detection tube 6 for storing detection reagents, namely a PCR tube, and the detection tube 6 is used for transferring the eluent containing nucleic acids in the elution storage tube 16 into the detection tube 6 to wait for detection.
The number of the washing storage pipes 15 is 2, and the 2 washing storage pipes 15 respectively store a first washing liquid for removing protein and carbohydrate macromolecular substances and a second washing liquid for removing salts. The number of the washing storage pipes 15 can be 3, and the 3 washing storage pipes 15 respectively store a first washing solution for removing protein, a second washing solution for removing saccharide macromolecular substances and a third washing solution for removing salts. The storage tube 2 further comprises a detection tube 6 for storing detection reagents, and the detection tube 6 is used for eluting the eluent containing nucleic acid in the storage tube 16 and transferring the eluent into the detection tube 6 to wait for detection. The storage tube 2 still includes the liquid sealing tube, and the intraductal storage of liquid sealing is used for carrying out the solution of sealing the pipe to each storage tube 2 after extracting, owing to need seal each storage tube 2 after extracting, so store the solution that can play sealed effect through setting up and seal.
As shown in fig. 2, the stirring sleeve 9 is a hollow tubular structure, the bottom of the stirring sleeve 9 is closed, the top of the stirring sleeve 9 is open, the inner cavity of the stirring sleeve 9 is used for the magnetic rod to extend into the stirring sleeve from top to bottom, so that the target capturing medium is adsorbed on the outer wall of the bottom of the stirring sleeve 9 under the action of the magnetic rod, and the stirring sleeve 9 is driven by the moving mechanism located at the upper part of the stirring sleeve to reciprocate up and down and stir the solution in which the stirring sleeve 9 is located.
First section fixed connection of stirring cover 9 has earring 10 of level setting, earring 10 is hollow annular structure, and the cover is established on the outer wall of stirring cover 9, the inner wall of earring 10 and the outer wall fixed connection of stirring cover 9, earring 10 is used for the lower extreme of moving mechanism's manipulator to support the upside at earring 10, make the degree of depth that stirring cover 9 was snatched every time the same, earring 10 still is used for when stirring cover 9 is in stirring storage tube 14, make earring 10 take on stirring storage tube 14, guarantee that the bottom of stirring cover 9 does not touch the bottom of stirring storage tube 14, avoid stirring cover 9 to be snatched the in-process by moving mechanism and produce the deformation.
Present stirring cover 9 all is 8 antithetical couplet structures, and 8 stirring covers 9 link together promptly, consequently once must carry out the liquid mixing of 8 hole sites, need load by hand before the experiment moreover, and the stirring cover 9 of this application is located stirring storage tube 14 to for solitary pipe fitting, pre-packaged in the reagent box, do not need load by hand, only do the liquid mixing of a hole site once, avoid extravagant.
In addition, the position is the part of snatching of manipulator above earrings 10 of stirring cover 9 of this application, hugs closely the upside of earrings 10 through the inside profile modeling structure of manipulator, can guarantee the coaxial of stirring cover 9 and manipulator, and snatchs reliable and stable, can not appear swaying from side to side for the condition that the axis of manipulator and the axis of stirring cover 9 are not coincident. The stirring sleeve 9 is grabbed by adopting an internal clamping mode in the prior art, the left-right swinging problem exists, the coaxiality cannot be guaranteed, and meanwhile, due to the structure without the ear rings 10, the bottom of the stirring sleeve 9 is easy to damage in the grabbing process of the stirring sleeve 9.
The invention also includes: support frame 4, support frame 4 includes: preceding curb plate 5, posterior lateral plate, left side board and right side board 8, the vertical setting of preceding curb plate 5, the upper end of preceding curb plate 5 and the lower border fixed connection of the front side of storing fixed plate 1, the vertical setting of posterior lateral plate, the upper end of posterior lateral plate and the lower border fixed connection of the rear side of storing fixed plate 1.
Left side board and the vertical setting of right side board 8, the upper end of left side board and right side board 8 respectively with store fixed plate 1 under the left side border and the right side along fixed connection, and mutually support with preceding curb plate 5 and posterior lateral plate and form the framework, and then support storing fixed plate 1 and each storage tube 2, when using, conveniently arrange the desktop in through preceding curb plate 5, the posterior lateral plate, the lower extreme of left side board and right side board 8, and guarantee that the level of storing fixed plate 1 is placed and the vertical of each storage tube 2 is placed. The lower extreme of preceding curb plate 5 and posterior lateral plate is seted up the recess from bottom to top, and two recesses are used for the experimenter to snatch and place.
According to the invention, the nucleic acid extraction reagent, the PCR reaction solution, the pipette tip and the stirring sleeve 9 are pre-packaged, the purpose of nucleic acid extraction and detection can be realized only by adding a sample by a client, no personnel intervention is needed in the whole process, the purpose of adding the sample by a laboratory technician is really realized, and the purpose of extraction and detection is automatically completed by an instrument; the invention realizes the transfer of the target capture medium to the detection tube 6 by the stirring sleeve 9 and the magnetic rod, completes the amplification and detection of nucleic acid by the detection tube 6, has seamless connection in the whole detection process, saves the process time of reagent preparation, sample addition and multiple consumable loading of an experimenter, can complete the detection without inserting different independent spaces by the same experimenter, and can shorten the detection time to 0.5 to 1 hour, while the prior art needs at least 2 hours.
The prepackaged nucleic acid extraction reagent and the PCR amplification reaction solution do not need to be prepared by a laboratory technician on site; the storage tube 2 is internally pre-packaged with a liquid-transfering gun head, the liquid-transfering function is automatically realized through an instrument liquid-transfering machine, and the manual liquid-transfering of a laboratory technician is not needed; the stirring sleeve 9 is pre-packaged in the storage tube 2, the reaction liquid uniformly mixing function is automatically realized through an instrument stirring mechanism, and a laboratory technician is not required to load consumables of the stirring sleeve 9; reserving the sample tube 11, wherein an experimenter only needs to add a sample into the sample tube 11, the consumable is put into equipment, and the preparation of the sample is completed by the equipment without human intervention; the invention can satisfy the requirement of completing nucleic acid extraction and detection at one time without setting a plurality of independent spaces, and reduces the requirement on an experimental field.
The invention also discloses a use method of the reagent extraction box for the nucleic acid detection system, and the reagent extraction box comprises the following steps: a storage fixing plate 1 and a plurality of storage tubes 2.
Store 1 level setting of fixed plate, store and seted up a plurality of storage holes 3 on the fixed plate 1, a plurality of storage tubes 2 are located the downside of storing fixed plate 1, the lower part of a plurality of storage tubes 2 is sealed, open-top, a plurality of storage tubes 2 respectively with the 3 coaxial settings of storage hole that correspond, the upper end opening of each storage tube 2 equals with the internal diameter of the storage hole 3 that corresponds and with store fixed plate 1 an organic whole and be connected, a plurality of storage tubes 2 are used for the required reagent of each step of storage nucleic acid detection.
The storage tube 2 is at least provided with 7, and 7 storage tubes are respectively a sample tube 11 used for containing a sample therein, a cracking storage tube 12 in which a cracking solution is stored, a medium storage tube 13 in which a target capture medium solution is stored, a washing storage tube 15 in which a washing solution is stored, an elution storage tube 16 in which an eluent is stored, a stirring storage tube 14 in which a stirring sleeve 9 is placed, and a pipette tip tube 7 in which a pipette tip is stored. The storage tube 2 further comprises a detection tube 6 for storing detection reagents, namely a PCR tube, and the detection tube 6 is used for transferring the eluent containing nucleic acids in the elution storage tube 16 into the detection tube 6 to wait for detection.
Therefore, the use method of the reagent extraction kit for the nucleic acid detection system disclosed by the invention comprises the following steps:
step S1: a mechanical hand is used for grasping the pipette tip in the pipette tip tube 7, a predetermined amount of sample is sucked from the sample tube 11, the sucked sample is placed into the cracking storage tube 12,
step S2: after the existing pipette tip of the manipulator is discarded, a new pipette tip in the pipette tip tube 7 is grabbed again, the solution of the target capture medium is sucked from the medium storage tube 13 and is placed in the cracking storage tube 12,
and step S3: after the existing pipette tip of the manipulator is discarded, the stirring sleeve 9 in the stirring storage tube 14 is grabbed, the stirring sleeve 9 is driven to move up and down in the cracking storage tube 12 to stir the sample, so that the cells in the sample are cracked to obtain nucleic acid and are adsorbed on the target capture medium, then the magnetic rod at the lower end of the manipulator is controlled to move downwards to extend into the stirring sleeve 9, so that the target capture medium is adsorbed on the outer wall at the lower end of the stirring sleeve 9 under the action of the magnetic rod,
and step S4: the stirring sleeve 9 absorbed with the target capture media is moved into the washing storage tube 15 by the manipulator, a magnetic rod at the lower end of the manipulator is controlled to move upwards and withdraw from the inner cavity of the stirring sleeve 9, so that the target capture media absorbed on the outer wall of the lower end of the stirring sleeve 9 are released and enter the washing storage tube 15,
step S5: the stirring sleeve 9 is controlled by a manipulator to move up and down in the washing storage tube 15 for stirring and washing, so that the washing liquid washes away proteins, carbohydrate macromolecular substances and salts on the surface of the nucleic acid adsorbed by the target capture medium;
step S6: the magnetic rod at the lower end of the manipulator and the stirring sleeve 9 are controlled to move the nucleic acid adsorbed by the target capture medium into the elution storage tube 16 again, and the stirring sleeve 9 is driven to move up and down to stir and elute, so that the nucleic acid adsorbed on the target capture medium is eluted by the eluent,
step S7: after the existing stirring sleeve 9 of the manipulator is discarded, a new pipette tip in the pipette tip tube 7 is sucked again, and the eluent containing nucleic acid in the elution storage tube 16 is sucked and placed in the detection tube 6 for detection.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent replacements, improvements, etc. within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A reagent extraction cartridge for a nucleic acid detecting system, comprising:
a storage fixing plate (1) which is horizontally arranged and is provided with a plurality of storage holes (3),
a plurality of storage tubes (2) which are positioned at the lower side of the storage fixing plate (1), the lower parts of the storage tubes are closed, the top parts of the storage tubes are opened, each storage tube (2) is coaxially arranged with the corresponding storage hole (3), the upper end opening of each storage tube (2) has the same inner diameter with the corresponding storage hole (3) and is integrally connected with the storage fixing plate (1) and is used for storing reagents required by each step of nucleic acid detection,
wherein, the storage tube (2) is provided with 7 at least, be used for holding sample tube (11) of sample in it respectively, the schizolysis storage tube (12) of storage lysis liquid in it, the media storage tube (13) of storage target capture media solution in it, washing storage tube (15) of storage washing liquid in it, elution storage tube (16) of storage eluent in it, stirring storage tube (14) of placing stirring cover (9) in it, rifle head pipe (7) of storage liquid-transfering rifle head in it.
2. The reagent cartridge according to claim 1, wherein 2 washing storage tubes (15) are provided, and two washing storage tubes (15) store a first washing solution for removing proteins and carbohydrates from macromolecular substances and a second washing solution for removing salts from macromolecular substances.
3. The reagent cartridge according to claim 1, wherein 3 washing storage tubes (15) are provided, and a first washing solution for removing proteins, a second washing solution for removing sugar-like macromolecular substances, and a third washing solution for removing salts are stored in each of the three washing storage tubes (15).
4. The reagent extraction cartridge for a nucleic acid detecting system according to claim 2 or 3, wherein the storage tube (2) further comprises a detection tube (6) for storing a detection reagent, and the detection tube (6) is used for eluting the elution solution containing the nucleic acid in the storage tube (16) and transferring the elution solution into the detection tube (6) to wait for detection.
5. The reagent extraction cartridge for a nucleic acid detection system according to claim 4, wherein the stirring sleeve (9) is a hollow tubular structure, the bottom of the stirring sleeve is closed, the top of the stirring sleeve is open, the inner cavity of the stirring sleeve (9) is used for allowing a magnetic rod to extend into the stirring sleeve from top to bottom, so that the target capture medium is adsorbed on the outer wall of the bottom of the stirring sleeve (9) under the action of the magnetic rod, and the stirring sleeve (9) is used for reciprocating up and down under the driving of a moving mechanism located at the upper part of the stirring sleeve and stirring the solution in which the stirring sleeve (9) is located.
6. The reagent extraction cartridge for a nucleic acid detection system according to claim 5, wherein the upper half section of the stirring sleeve (9) is fixedly connected with a horizontally arranged ear ring (10), the ear ring (10) is of a hollow ring structure and is sleeved on the outer wall of the stirring sleeve (9), the inner wall of the ear ring (10) is fixedly connected with the outer wall of the stirring sleeve (9), the lower end of the manipulator of the ear ring (10) for the moving mechanism abuts against the upper side of the ear ring (10), so that the depth of the stirring sleeve (9) to be grasped each time is the same, and when the stirring sleeve (9) is in the stirring storage tube (14), the ear ring (10) is lapped on the stirring storage tube (14), the bottom of the stirring sleeve (9) is ensured not to touch the bottom of the stirring storage tube (14), and deformation of the stirring sleeve (9) in the process of being grasped by the moving mechanism is avoided.
7. The reagent cartridge according to claim 6, wherein the storage tubes (2) further comprise a liquid sealing tube in which a solution for sealing each storage tube (2) after completion of extraction is stored.
8. The reagent extraction cartridge for a nucleic acid detecting system according to claim 7, characterized by further comprising: a supporting frame (4),
the support frame (4) comprises:
a front side plate (5) which is vertically arranged, the upper end of the front side plate is fixedly connected with the lower edge of the front side of the storage fixing plate (1),
a rear side plate which is vertically arranged, the upper end of the rear side plate is fixedly connected with the lower edge of the rear side of the storage fixing plate (1),
left side board and right side board (8), vertical setting, its upper end respectively with store the left side of fixed plate (1) lower border and right side lower border fixed connection, and mutually support with preceding curb plate (5) and posterior lateral plate and form the framework, and then support storing fixed plate (1) and each storage tube (2), when using, conveniently through preceding curb plate (5), the posterior lateral plate, the lower extreme of left side board and right side board (8) is arranged in on the desktop, and guarantee that the level of storing fixed plate (1) is placed and the vertical of each storage tube (2) is placed.
9. The reagent extraction cassette for nucleic acid detecting system according to claim 8, wherein the lower ends of the front side plate (5) and the rear side plate are provided with grooves from bottom to top, and the two grooves are used for holding and placing by the tester.
10. A method of using a reagent extraction cartridge for a nucleic acid detection system, comprising:
a storage fixing plate (1) which is horizontally arranged and is provided with a plurality of storage holes (3),
a plurality of storage tubes (2) which are positioned at the lower side of the storage fixing plate (1), the lower parts of the storage tubes are closed, the top parts of the storage tubes are opened, each storage tube (2) is coaxially arranged with the corresponding storage hole (3), the upper end opening of each storage tube (2) has the same inner diameter with the corresponding storage hole (3) and is integrally connected with the storage fixing plate (1) and is used for storing reagents required by each step of nucleic acid detection,
the device comprises a storage tube (2), a sample tube (11), a cracking storage tube (12), a medium storage tube (13), a washing storage tube (15), an elution storage tube (16), a stirring storage tube (14) and a pipette tip tube (7), wherein the storage tube (2) is at least provided with 7 storage tubes, the sample tube (11) is used for containing samples, the cracking storage tube (12) is stored with cracking liquid, the medium storage tube (13) is stored with target capture medium solution, the washing storage tube (15) is stored with washing liquid, the elution storage tube (16) is stored with eluent, the stirring storage tube (14) is placed with a stirring sleeve (9), and the pipette tip tube (7) is stored with pipette tips;
the using method comprises the following steps:
step S1: a mechanical hand is used for grabbing the pipette head in the pipette head tube (7), a preset amount of samples are sucked from the sample tube (11), the sucked samples are placed into a cracking storage tube (12),
step S2: after the existing pipette tip of the manipulator is discarded, a new pipette tip in the pipette tip tube (7) is grabbed again, the solution of the target capture medium is sucked from the medium storage tube (13) and is placed in the cracking storage tube (12),
and step S3: after the existing pipette tip of the manipulator is discarded, the stirring sleeve (9) in the stirring storage tube (14) is grabbed, the stirring sleeve (9) is driven to move up and down in the cracking storage tube (12) to stir the sample so that the cells in the sample are cracked into nucleic acid and adsorbed on the target capture medium, then the magnetic rod at the lower end of the manipulator is controlled to move down and stretch into the stirring sleeve (9) so that the target capture medium is adsorbed on the outer wall at the lower end of the stirring sleeve (9) under the action of the magnetic rod,
and step S4: the mechanical arm is utilized to move the stirring sleeve (9) adsorbed with the target capture medium into the washing storage tube (15), the magnetic rod at the lower end of the mechanical arm is controlled to move upwards to evacuate out of the inner cavity of the stirring sleeve (9), so that the target capture medium adsorbed on the outer wall of the lower end of the stirring sleeve (9) is released and enters the washing storage tube (15),
step S5: the stirring sleeve (9) is controlled by a manipulator to move up and down in the washing storage tube (15) for stirring and washing, so that the washing liquid washes away proteins, saccharides and macromolecular substances and salts on the surface of the nucleic acid adsorbed by the target capture medium;
step S6: the magnetic rod and the stirring sleeve (9) at the lower end of the manipulator are controlled to move the nucleic acid adsorbed by the target capture medium into the elution storage tube (16) again, and the stirring sleeve (9) moves up and down to stir and elute, so that the elution solution elutes the nucleic acid adsorbed on the target capture medium,
step S7: after the existing stirring sleeve (9) of the manipulator is discarded, a new liquid-transfer gun head in the gun head tube (7) is sucked again, and eluent containing nucleic acid in the elution storage tube (16) is sucked and placed in the detection tube (6) to wait for detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211240655.7A CN115637266A (en) | 2022-10-11 | 2022-10-11 | Reagent extraction box for nucleic acid detection system and use method thereof |
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| CN202211240655.7A CN115637266A (en) | 2022-10-11 | 2022-10-11 | Reagent extraction box for nucleic acid detection system and use method thereof |
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| CN202211240655.7A Pending CN115637266A (en) | 2022-10-11 | 2022-10-11 | Reagent extraction box for nucleic acid detection system and use method thereof |
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| CN (1) | CN115637266A (en) |
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