CN115607651A - A kind of hypoglycemic traditional Chinese medicine composition - Google Patents

Abstract

The invention provides a traditional Chinese medicine composition for reducing blood sugar, which belongs to the technical field of traditional Chinese medicine preparations, and is prepared from 10-20 parts of rhizoma polygonati, 1-5 parts of liquorice, 5-10 parts of polygonatum odoratum, 10-20 parts of radix puerariae, 10-25 parts of Chinese yam, 5-25 parts of mulberry leaves, 5-10 parts of mulberry fruits, 5-10 parts of hawthorn, 10-25 parts of jerusalem artichoke, 5-25 parts of poria cocos and 1-5 parts of ginseng peptides. The auxiliary treatment of the non-insulin-dependent diabetes has satisfactory curative effect, can better assist in reducing blood sugar, and has no side effect in treatment.

Description

A kind of hypoglycemic traditional Chinese medicine composition

technical field

The invention belongs to the technical field of traditional Chinese medicine preparations, and in particular relates to a hypoglycemic traditional Chinese medicine composition.

Background technique

Diabetes mellitus is a group of clinical syndromes caused by the interaction of genetic and environmental factors. Diabetes has a very large prevalence worldwide and there is no very good way to treat it. Ginseng is one of the special and precious medicinal materials in my country. In recent years, more and more scholars have turned their attention to the effect of ginseng on lowering blood sugar. Although ginseng cannot replace insulin, it can relieve symptoms and play a very beneficial auxiliary role. Ginseng also has the effect of resisting thirst, deficiency of qi and yin, fatigue and hypoxia. However, long-term or excessive use of ginseng can cause many adverse effects.

Contents of the invention

The object of the present invention is to provide a kind of hypoglycemic traditional Chinese medicine composition, compatibility is reasonable, raw material source is wide, and preparation method is simple, and this hypoglycemic traditional Chinese medicine composition is by glucose-6-phosphatase (G-6-Pase), glucokinase ( GK) activity, has hypoglycemic effect.

A hypoglycemic traditional Chinese medicine composition, which is prepared from the following raw materials in parts by weight: 10-20 parts of Polygonatum, 1-5 parts of Licorice, 5-10 parts of Polygonatum Polygonatum, 10-20 parts of Pueraria lobata, 10-25 parts of Chinese yam, mulberry 5-25 parts of leaves, 5-10 parts of mulberries, 5-10 parts of hawthorn, 10-25 parts of Jerusalem artichoke, 5-25 parts of poria cocos and 1-5 parts of ginseng peptide.

The ginseng peptide is crude ginseng peptide, and the preparation process of the crude ginseng peptide is as follows: take a certain amount of ginseng slices, crush them, pass through a 40-mesh sieve to obtain ginseng powder, extract 8 times the amount of water for 3 times, each time for 12 hours, and filtrate three times Combine, concentrate the extract to 1/6 of the original volume, heat the concentrate to 60°C at a rate of 2°C/min, add a mixed enzyme of β-glucanase and pepsin with a mass ratio of 1:2.5, mix The mass ratio of enzyme to ginseng slices is 1:175 to obtain a mixed solution, adjust the pH value of the mixed solution to 5.5, enzymatically hydrolyze at 45°C for 50 minutes, add β-glucanase, the mass ratio of β-glucanase to ginseng powder 1:210, enzymatically hydrolyzed at 45°C for 50 minutes, then heated to boiling, kept for 8 minutes to inactivate the enzyme, naturally cooled to room temperature, adjusted to neutral pH, and freeze-dried to obtain crude ginseng peptide.

The ginseng peptide is a ginseng extract peptide, and the preparation process of the ginseng extract peptide is as follows:

① Take a certain amount of ginseng slices, crush them, and pass through a 40-mesh sieve to obtain ginseng powder. Extract 8 times the amount of water for 3 times, each time for 12 hours. Raise the temperature to 60°C at a rate of 2°C/min, add a mixed enzyme of β-glucanase and pepsin with a mass ratio of 1:2.5, and the mass ratio of the mixed enzyme to ginseng slices is 1:175 to obtain a mixed solution, adjust The pH value of the mixture is 5.5, enzymatic hydrolysis at 45°C for 50 minutes, adding β-glucanase, the mass ratio of β-glucanase to ginseng powder is 1:210, enzymatic hydrolysis at 45°C for 50 minutes, and then heat up to boiling. And keep it for 8 minutes to inactivate the enzyme, naturally cool to room temperature, adjust the pH value to be neutral, and freeze-dry to obtain the crude ginseng peptide;

② Mix the crude ginseng peptide, ethyl acetate and acetone according to the volume ratio of 2:1:1, add Na ₂ CO ₃ with a volume percentage concentration of 5% for extraction, take the upper layer, evaporate the organic solvent to dryness, and freeze-dry to obtain Ginseng Extract Peptides.

Through the above-mentioned design scheme, the present invention can bring the following beneficial effects: the hypoglycemic traditional Chinese medicine composition proposed by the present invention has reasonable compatibility, wide source of raw materials, and simple preparation method. ml, it shows a good effect on glucose consumption and cell survival rate, thereby playing a role in hypoglycemia, clinical application also shows that adjuvant treatment of non-insulin-dependent diabetes has a satisfactory curative effect, can better Auxiliary hypoglycemic, no side effects occurred during treatment.

Description of drawings

Fig. 1 is the effect diagram of the toxicity test of ginseng series and bovine bone peptide on liver cancer HepG2 cells in the embodiment of the present invention;

Fig. 2 is the effect diagram of toxicity test of two kinds of hypoglycemic products in the embodiment of the present invention to liver cancer HepG2 cell;

Fig. 3 is an effect diagram of the effect of ginseng series and bovine bone peptide on the glucose consumption in the culture medium of liver cancer HepG2 cells in the embodiment of the present invention;

Figure 4 is an effect diagram of the effect of two hypoglycemic products on the glucose consumption in the culture medium of liver cancer HepG2 cells in the embodiment of the present invention;

Figure 5 is an effect diagram of the effect of liver cancer HepG2 cells cultured by ginseng series and bovine bone peptide on glucose-6-phosphatase (G-6-Pase) in the embodiment of the present invention;

6 is an effect diagram of the effect of liver cancer HepG2 cells cultured by two hypoglycemic products on glucose-6-phosphatase (G-6-Pase) in the embodiment of the present invention;

Figure 7 is an effect diagram of the effect of liver cancer HepG2 cells cultured by ginseng series and bovine bone peptide on glucose-6-phosphatase glucokinase (GK) in the embodiment of the present invention;

Fig. 8 is a graph showing the effects of two kinds of hypoglycemic products on glucose-6-phosphatase glucokinase (GK) in cultured liver cancer HepG2 cells in the embodiment of the present invention.

detailed description

In order to enable those skilled in the art to better understand the technical solutions in the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described The embodiments are only some of the embodiments of the present invention, not all of them.

A hypoglycemic traditional Chinese medicine composition, which is prepared from the following raw materials in parts by weight: 10-20 parts of Polygonatum, 1-5 parts of Licorice, 5-10 parts of Polygonatum Polygonatum, 10-20 parts of Pueraria lobata, 10-25 parts of Chinese yam, mulberry 5-25 parts of leaves, 5-10 parts of mulberries, 5-10 parts of hawthorn, 10-25 parts of Jerusalem artichoke, 5-25 parts of poria cocos and 1-5 parts of ginseng peptide. The hypoglycemic traditional Chinese medicine composition provided by the invention has reasonable compatibility, wide sources of raw materials, simple preparation method, and hypoglycemic effect. Clinical application also shows that adjuvant treatment of non-insulin-dependent diabetes mellitus has satisfactory curative effect, can assist in lowering blood sugar, and no side effects have been seen in the treatment. It should be noted that, for the convenience of description, the hypoglycemic traditional Chinese medicine composition proposed in the present invention is named "Polygonatum Jerusalem artichoke and Ginseng Peptide".

The analysis method of ginseng series and its products for hypoglycemic, adopts low temperature extraction combined with enzymatic hydrolysis to obtain ginseng crude peptides, and the ginseng crude peptides are further extracted and decolorized to obtain ginseng extract peptides and ginseng decolorized peptides, each ginseng series of crude peptides and their products, Inonotus obliquus was tested for its effect on the viability of human liver cancer cells (HepG2) by the methyl thiazolyl tetrazolium (MTT) test, and the effect of ginseng crude peptide series and its products on glucose-6-phosphatase (G-6-Pase) , The effect of glucokinase (GK) activity.

The analysis method of ginseng series and hypoglycemic products, the specific process is as follows:

1. Preliminary preparation

Preparation of Ginseng Series Samples

Preparation of ginseng samples: take a certain amount of ginseng slices, crush them, and pass through a 40-mesh sieve to obtain ginseng powder; 8 times the amount of water extraction for 3 times, each time for 12 hours, combine the three filtrates, and freeze-dry to obtain ginseng samples;

Preparation of crude ginseng peptide samples: take a certain amount of ginseng slices, crush them, pass through a 40-mesh sieve to obtain ginseng powder, extract 8 times the amount of water for 3 times, each time for 12 hours, combine the three filtrates, and concentrate the extract to 1/2 of the original volume. 6. Heat the concentrated solution to 60°C at a rate of 2°C/min, add a mixed enzyme of β-glucanase and pepsin with a mass ratio of 1:2.5, and the mass ratio of the mixed enzyme to ginseng slices is 1:175, Get the mixed solution, adjust the pH value of the mixed solution to 5.5, enzymatically hydrolyze at 45°C for 50 minutes, add β-glucanase, the mass ratio of β-glucanase to ginseng powder is 1:210, and enzymolyze at 45°C for 50 minutes Afterwards, the temperature was raised to boiling, and kept for 8 minutes to inactivate the enzyme, naturally cooled to room temperature, adjusted to neutral pH, and freeze-dried to obtain the crude ginseng peptide.

Preparation of ginseng extracted peptide samples: mix the crude ginseng peptide, ethyl acetate, and acetone at a volume ratio of 2:1:1, add 5% Na ₂ CO ₃ for extraction, take the upper layer, and evaporate the organic solvent to dryness Freeze-dry to obtain the ginseng extract peptide.

Preparation of ginseng decolorizing peptide samples: add 4% H ₂ O ₂ to decolorize crude ginseng peptide at 60° C. for 60 minutes, freeze-dry to obtain ginseng decolorizing peptide.

Preparation of Polygonatum Polygonatum and Ginseng Peptides for Hypoglycemic Products

Prepare Polygonatum Jerusalem artichoke ginseng peptide to the required concentration, precisely weigh 1mg of Polygonatum Jerusalem artichoke ginseng peptide, add 1ml of cell culture medium to dissolve completely, pass through 0.2mm filter membrane to obtain 1mg/ml mother liquor, and obtain Polygonatum Jerusalem artichoke ginseng peptide sample.

Preparation of Inonotus obliquus for comparison of hypoglycemic products

Prepare the Inonotus obliquus to the desired concentration, precisely weigh 1 mg of Inonotus obliquus, add 1 ml of cell culture medium to completely dissolve it, and pass through a 0.2 mm filter to obtain a 1 mg/ml mother liquor, which is the sample of Inonotus obliquus.

Bovine bone peptide is commercially available.

2. Experimental analysis

HepG2 cells in the logarithmic growth phase were inoculated into 96-well culture plates at ⁴ ×10 cells/well, 200 μl per well, cultured in a 37°C, 5% _CO2 incubator for 24 hours, discarded the supernatant, and added concentrations of 12.5μg/ml, 25μg/ml, 50μg/ml, 100μg/ml, 200μg/ml sample solution, 5 duplicate wells in each group, discard the culture solution after 24h of culture, add MTT (5mg/ml) 100μl to each well, 4h Then add 100 μl dimethyl sulfoxide (DMSO), shake for 10 min, and detect the absorbance at 490 nm; calculate the cell viability of each group of samples with different concentrations.

Wash the medium-well plate of the kit first, fill each well with washing solution, let it stand for 1 min, shake off the washing solution in the well, pat dry on filter paper, and wash 5 times in this way. Add 50 μL of standard products in the kit of glucokinase (GK) and glucose-6-phosphatase (G-6-Pase) of different concentrations in the designated standard wells, add 10 μL of ultrasonically disrupted cell samples into the designated wells of samples, and then Add 40 μL of the sample diluent in the glucokinase (GK) and glucose-6-phosphatase (G-6-Pase) kit, and use it as a blank control. In addition to the blank control wells, horseradish peroxidase (HRP) and 100 μL of labeled detection antibody were added to the wells, and the wells were sealed with a sealing film, and reacted in water at 37°C for 60 min. Discard the liquid and pat dry, then add washing solution, let it stand for 1min, discard the washing solution, pat dry, repeat this method 5 times. Add 50 μL each of the substrates A and B in the glucokinase (GK) and glucose-6-phosphatase (G-6-Pase) kits to each well, and incubate at 37°C in the dark for 15 minutes. Add 50 μL of stop solution in the glucokinase (GK) and glucose-6-phosphatase (G-6-Pase) kits to each well, measure the absorbance value at 450 nm with a microplate reader within 15 minutes, and measure glucose according to the ELISA instructions. Kinase (GK), glucose-6-phosphatase (G-6-Pase) activity.

Steps:

1. Adding samples of standard products: set standard product wells and sample wells, and add 50 μL of standard products in the kit of glucokinase (GK) and glucose-6-phosphatase (G-6-Pase) of different concentrations to each of the standard product wells;

2. Addition: set up blank control wells (no samples and enzyme-labeled reagents are added to the blank control wells, and the rest of the steps are the same), and sample wells to be tested. Add 40 μL of the sample diluent in the glucokinase (GK) and glucose-6-phosphatase (G-6-Pase) kit to the wells of the sample to be tested on the enzyme-labeled plate, and then add 10 μL of the sample to be tested. Add the sample to the bottom of the microplate well, shake gently to mix;

3. Add enzyme: Add 100 μL of enzyme-labeled reagent to each well, except the blank control well;

4. Incubation: Seal the plate with a sealing film and incubate at 37°C for 60 minutes;

5. Dosing: Dilute 20 times concentrated washing liquid with distilled water 20 times before use;

6. Washing: Carefully peel off the sealing film, discard the liquid, shake dry, fill each well with washing liquid, let it stand for 30 seconds, then discard, repeat this 5 times, and pat dry;

7. Color development: First add 50 μL of chromogenic agent A in the glucokinase (GK) and glucose-6-phosphatase (G-6-Pase) kit to each well, then add 50 μL of chromogenic agent B, shake and mix gently , 37°C for 15 minutes in the dark;

8. Termination: Add 50 μL of the stop solution in the glucokinase (GK) and glucose-6-phosphatase (G-6-Pase) kit to each well to stop the reaction (the blue color turns yellow immediately);

9. Determination: Set the blank control well to zero, and measure the absorbance (OD value) of each well in sequence at a wavelength of 450 nm. The determination should be carried out within 15 minutes after adding the stop solution in the glucokinase (GK) and glucose-6-phosphatase (G-6-Pase) kit.

HepG2 cells in the logarithmic growth phase were inoculated into 96-well culture plates at ⁴ ×10 cells/well, 200 μl per well, cultured in a 37°C, 5% _CO2 incubator for 24 hours, discarded the supernatant, and added concentrations of 12.5μg/ml, 25μg/ml, 50μg/ml, 100μg/ml, 200μg/ml sample solution, 5 duplicate wells in each group, discard the culture solution after 24h of culture, add MTT (5mg/ml) 100μl to each well, 4h Then add 100 μl DMSO, shake for 10 min, detect the absorbance at 490 nm, and calculate the cell viability of each group of samples with different concentrations.

HepG2 cells in the logarithmic growth phase were inoculated in a 12-well culture plate at ⁵ ×10 cells/well, 1 ml per well, cultured in a 37°C, 5% CO ₂ incubator for 24 hours, discarded the supernatant, and added 1 μg/ml LPS Stimulate the cells for 24 hours, set up a blank control group, an LPS model group, and a sample solution group with a concentration of 25 μg/ml, 50 μg/ml, and 100 μg/ml, respectively, and each group has 3 duplicate wells. Glucose consumption and glucokinase (GK), glucose-6-phosphatase (G-6-Pase) activity.

The experimental results are expressed as x±s, and SPSS 21.0 statistical software is used for paired t test and one-way analysis of variance. When P<0.05, there is a statistical difference.

Embodiment one

Analysis methods for ginseng series and hypoglycemic products, including:

Step 1. Preliminary preparation

Take a certain amount of ginseng slices, crush them, pass through a 40-mesh sieve to obtain ginseng powder, extract 8 times the amount of water for 3 times, each time for 12 hours, take the filtrate (preserved at low temperature), combine the three filtrates, and freeze-dry to obtain ginseng. Sample, yield 11.4%;

Take a certain amount of ginseng slices, crush them, and pass through a 40-mesh sieve to obtain ginseng powder, extract 8 times the amount of water for 3 times, each time for 12 hours, take the filtrate (preserved at low temperature), combine the three filtrates, and concentrate the extract to 1% of the original volume. /6. The first enzymatic hydrolysis: heat the concentrated solution to 60°C at a rate of 2°C/min, add a mixed enzyme of β-glucanase and pepsin with a mass ratio of 1:2.5, and the mass ratio of the mixed enzyme to ginseng slices is 1:175 to obtain a mixed solution, adjust the pH value of the mixed solution to 5.5, enzymatically hydrolyze at 45°C for 50 minutes, add β-glucanase and ginseng powder at a mass ratio of 1:210, and heat up to Boil and keep for 8 minutes to inactivate the enzyme, naturally cool to room temperature, adjust the pH value to be neutral, and freeze-dry to obtain the crude ginseng peptide with a yield of 10.48%; mix the crude ginseng peptide, ethyl acetate, and acetone (2: 1:1, V/V/V), adding Na ₂ CO ₃ (5%, V/V) for extraction, taking the upper layer, evaporating the organic solvent and then freeze-drying to obtain the ginseng extract peptide with a yield of 8.38%; at 60 The ginseng crude peptide was decolorized by adding H ₂ O ₂ (4%, V/V) for 60 min at ℃, and freeze-dried to obtain the ginseng decolorized peptide with a yield of 4.61%;

Bovine bone peptide is commercially available.

Step 2, preparing hypoglycemic products

Polygonatum Jerusalem artichoke ginseng peptide and Inonotus obliquus are directly prepared to the required concentration, and 1 mg of Polygonatum Jerusalem artichoke ginseng peptide is precisely weighed, and 1 ml of cell culture medium is added to completely dissolve it and pass through a 0.2 mm filter membrane to obtain a 1 mg/ml mother liquor; Add 1ml of cell culture medium to dissolve 1mg of Inonotus obliquus and pass through a 0.2mm filter to obtain 1mg/ml mother liquor; two samples of hypoglycemic products are obtained.

Step 3. Toxicity test of ginseng series and bovine bone peptide on liver cancer HepG2 cells

As shown in Figure 1, when the concentration of ginseng series is 12.5 μg/ml, all cells except garden ginseng cells have significant inhibitory effect on proliferation; when the concentration is 25 μg/ml, except garden ginseng and ginseng crude peptides are Cells have a significant proliferation effect (P<0.05). At 100 μg/ml, the effect of ginseng crude peptide on cell proliferation was significantly higher than that of garden ginseng, ginseng decolorized peptide, and ginseng extract peptide; when the concentration was 200 μg/ml, ginseng crude peptide There was a significant proliferation effect (P<0.05). The viability of the cultured cells is good, and there is no inhibitory effect on the proliferation of the liver cancer HepG2 cells, so it can be used to culture the liver cancer HepG2 cells for the study of hypoglycemic experiments.

Step 4. Effect of hypoglycemic products on the viability of liver cancer HepG2 cells

As shown in Figure 2, when the concentration is 12.5-25μg/ml, the two hypoglycemic products have no significant effect on the viability of the cells, and when the concentration is 50-200μg/ml, both have a significant effect on the cells Proliferation (P<0.05); at 50 μg/ml, the effect of Polygonatum artichoke and ginseng peptide on cell proliferation was higher than that of Inonotus obliquus. The cell viability of the cultured cells is good, and has no inhibitory effect on the proliferation of the liver cancer HepG2 cells, so it can be used to culture the liver cancer HepG2 cells for the study of hypoglycemic experiments.

Step 5. Effect of ginseng series and bovine bone peptide on glucose consumption in culture medium of liver cancer HepG2 cells

As shown in Figure 3, the hypoglycemic effect was judged by measuring the glucose consumption in the cell culture medium, and the more glucose consumption in the cell culture medium, the better the hypoglycemic effect of the cells. Compared with the blank control group, the consumption of glucose in the model group was significantly increased (P<0.05). At 25 μg/ml, the release of glucose consumption in each administration group was significantly increased compared with the model group (P<0.05); at 50μg/ml, except for garden ginseng, other groups can significantly increase the consumption of glucose compared with the model group (P<0.05), there is no difference among the groups; at 100μg/ml , compared with the model group, the five administration groups could significantly increase the consumption of glucose (P<0.05). Ginseng in the garden.

Step 6. Effect of hypoglycemic products on glucose consumption in culture medium of liver cancer HepG2 cells

As shown in Figure 4, compared with the blank control group, the consumption of glucose in the model group has a significant increase (P<0.05), and at 25-100 μg/ml, each group has a significant increase compared with the model group ( P<0.05), at 25μg/ml, Jerusalem artichoke ginseng peptide was superior to Inonotus obliquus (P<0.05); at 50-100μg/ml, there was no statistical difference between the two.

Step 7. Effects of ginseng series and bovine bone peptide on glucose-6-phosphatase (G-6-Pase) in cultured liver cancer HepG2 cells

As shown in Figure 5, after the cultured liver cancer HepG2 cells were broken and treated, the enzyme activity of the liver cancer HepG2 cells to glucose-6-phosphatase was determined, because glucose-6-phosphatase has a decomposition effect on glucose-6-phosphate, resulting in Produce glucose, so the lower the enzyme activity, the better the hypoglycemic effect. Compared with the blank control group, the model group had no significant decrease. Compared with the model group, at 25-100 μg/ml, the G-6-Pase activity of garden ginseng was significantly increased (P<0.05), and other ginseng groups had reduce.

Step 8. Effect of HepG2 cells cultured by hypoglycemic products on glucose-6-phosphatase (G-6-Pase)

As shown in Figure 6, after the cultured liver cancer HepG2 cells were broken and treated, the enzyme activity of the liver cancer HepG2 cells to glucose-6-phosphatase was determined, because glucose-6-phosphatase has a decomposition effect on glucose-6-phosphate, resulting in Produce glucose, so the lower the enzyme activity, the better the hypoglycemic effect. Compared with the blank control group, the model group had no significant decrease (P<0.05). Compared with the model group, the G-6-Pase activity was significantly increased (P<0.05) at 25 μg/ml. At 100μg/ml, Polygonatum artichoke ginseng peptide and Inonotus obliquus both significantly reduced the activity of G-6-Pase (P<0.05).

Step 9. Effects of HepG2 cells cultured with ginseng series and bovine bone peptide on glucose-6-phosphatase glucokinase (GK)

As shown in Figure 7, after the cultured liver cancer HepG2 cells were crushed, the enzyme activity of the liver cancer HepG2 cells to glucokinase was measured. Because glucokinase has a decomposing effect on glucose, the higher the enzyme activity, the better the hypoglycemic effect. Compared with the blank control group, GK in the model group was significantly increased (P<0.05). Compared with the blank control group, TNF-α was significantly increased in the model group (P<0.05). Compared with the model group, 25 μg At 50 μg/ml, the GK activity of the ginseng, ginseng crude peptide, and bovine osseopeptide groups increased significantly (P<0.05), and the effect of ginseng was the best (P<0.05); Sexually increased GK activity (P<0.05), and bovine bone peptide was better than garden ginseng (P<0.05); at 100μg/ml, garden ginseng had the best effect (P<0.05).

Step ten, the effect of hepatoma HepG2 cells cultured by hypoglycemic products on glucose-6-phosphatase glucokinase (GK)

As shown in Figure 8, after the cultured liver cancer HepG2 cells were crushed, the enzymatic activity of the liver cancer HepG2 cells on glucokinase was measured. Because glucokinase can decompose glucose, the higher the enzyme activity, the better the hypoglycemic effect. Compared with the blank control group, GK in the model group was significantly increased (P<0.05). At 25-100 μg/ml, the GK activity of Rhizoma Polygonatum and Jerusalem artichoke ginseng peptide was significantly higher than that of Inonotus obliquus (P<0.05).

Claims (3)

1. A hypoglycemic Chinese medicine composition, characterized in that the hypoglycemic Chinese medicine composition is prepared from the following raw materials in parts by weight: Polygonatum 10-20 parts, Radix Glycyrrhiza 1-5 parts, Polygonatum Polygonatum 5-10 parts, Radix Puerariae 10-20 parts, 10-25 parts of yam, 5-25 parts of mulberry leaves, 5-10 parts of mulberry fruit, 5-10 parts of hawthorn, 10-25 parts of Jerusalem artichoke, 5-25 parts of poria cocos and 1-5 parts of ginseng peptide. 2. The hypoglycemic Chinese medicine composition according to claim 1, characterized in that: the ginseng peptide is crude ginseng peptide, and the preparation process of the crude ginseng peptide is as follows: take a certain amount of ginseng slices, pulverize them, and pass through a 40-mesh sieve to obtain ginseng Powder, 8 times the amount of water leaching 3 times, each time for 12 hours, the three filtrates were combined, the extract was concentrated to 1/6 of the original volume, the concentrated solution was heated to 60 °C at a rate of 2 °C/min, and the mass ratio was 1:2.5 mixed enzyme of β-glucanase and pepsin, the mass ratio of mixed enzyme to ginseng slices is 1:175 to obtain a mixed solution, adjust the pH value of the mixed solution to 5.5, enzymatically hydrolyze at 45°C for 50 minutes, add β -Glucanase, the mass ratio of β-glucanase to ginseng powder is 1:210, heat up to boiling at 45°C for 50 minutes, keep for 8 minutes to inactivate the enzyme, cool naturally to room temperature, and adjust the pH The value is neutral, and freeze-dried to obtain the crude ginseng peptide. 3. The hypoglycemic traditional Chinese medicine composition according to claim 1, characterized in that: the ginseng peptide is a ginseng extract peptide, and the preparation process of the ginseng extract peptide is as follows: ① Take a certain amount of ginseng slices, crush them, and pass through a 40-mesh sieve to obtain ginseng powder. Extract 8 times the amount of water for 3 times, each time for 12 hours. Raise the temperature to 60°C at a rate of 2°C/min, add a mixed enzyme of β-glucanase and pepsin with a mass ratio of 1:2.5, and the mass ratio of the mixed enzyme to ginseng slices is 1:175 to obtain a mixed solution, adjust The pH value of the mixture is 5.5, enzymatic hydrolysis at 45°C for 50 minutes, adding β-glucanase, the mass ratio of β-glucanase to ginseng powder is 1:210, enzymatic hydrolysis at 45°C for 50 minutes, and then heat up to boiling. And keep it for 8 minutes to inactivate the enzyme, naturally cool to room temperature, adjust the pH value to be neutral, and freeze-dry to obtain the crude ginseng peptide; ② Mix the crude ginseng peptide, ethyl acetate and acetone according to the volume ratio of 2:1:1, add Na ₂ CO ₃ with a volume percentage concentration of 5% for extraction, take the upper layer, evaporate the organic solvent to dryness, and freeze-dry to obtain Ginseng Extract Peptides.

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