CN115467092A - Preparation method of supported dimethyloxalglycine nanofiber - Google Patents

Abstract

The invention discloses a method for preparing nanofibers loaded with dimethyloxalylglycine. The preparation steps are as follows: (1) adding polycaprolactone and dimethyloxalylglycine to a mixture of dimethylformamide and tetrahydrofuran Stirring in a solvent for 8-14 hours at a temperature of 20-25 degrees Celsius to obtain a uniform and stable solution; (2) Electrospinning the prepared solution to obtain a nanofiber membrane. The invention utilizes the combination of dimethyloxalylglycine and nanofibers to obtain materials beneficial to wound healing; and the method is low in cost, clean, green and efficient.

Description

A preparation method of loading dimethyloxalylglycine nanofibers

technical field

The invention relates to a method for preparing nanofibers, in particular to a method for preparing nanofibers loaded with dimethyloxalylglycine.

Background technique

The physiological microenvironment of refractory wounds is complex. A variety of different nanofibers have been prepared for different wound repairs, such as oriented nanofibers, drug-loaded nanofibers, high-function and core-shell nanofibers. However, traditional nanofiber dressings have limited promotion of wound healing, and most functional nanofibers only play a single role in wound repair (such as antibacterial, reducing body fluid loss, promoting repair, etc.), which is difficult to meet clinical needs.

Wound healing is a complex dynamic process involving cell migration, proliferation, differentiation, release of various cytokines, synthesis and remodeling of extracellular matrix (ECM). Therefore, it is of great significance to prepare repair materials with high bioactivity and rapid repair of refractory wounds.

Contents of the invention

In order to solve the problems of the prior art, the present invention provides a preparation method for loading dimethyloxalylglycine nanofibers, the nanofiber membrane dressing is based on a mixture of polycaprolactone and dimethyloxalylglycine , prepared by electrospinning technology to obtain nano-core fibers loaded with dimethyloxalylglycine. This nanofibrous membrane dressing is beneficial for the healing of chronic ischemic wounds.

The technical solution adopted by the present invention to solve the problems of the technologies described above is:

A preparation method for loading dimethyloxalylglycine nanofibers, comprising the steps of:

(1) Add polycaprolactone (PCL) and dimethyloxalylglycine (DMOG) to a mixed solvent of dimethylformamide and tetrahydrofuran, and then place it in a magnetic stirrer at 20-25 degrees Celsius Stir at high temperature for 8-14 hours to obtain a uniform and stable solution;

(2) Pour the prepared solution into a syringe needle tube, fix it on an electrospinning device, and perform electrospinning to obtain a nanofiber membrane.

Based on the above technical solution, preferably, in step (2), the electrospinning voltage is 6-10kv, and the ejection flow rate is 0.5-0.7ml/h.

Based on the above technical solution, preferably, in step (1), the mass ratio of polycaprolactone to dimethyloxalylglycine is 0.9002:0.3850.

Based on the above technical scheme, preferably, in step (1), the volume ratio of dimethylformamide to tetrahydrofuran is 3:1.

Based on the above technical scheme, preferably, in step (1), the ratio of the mixed solvent of polycaprolactone, dimethylformamide and tetrahydrofuran is 0.9002g: 4ml.

Based on the above technical solution, preferably, in step (2), the rotating speed of the receiving drum is 500-600r/min.

Based on the above technical solution, preferably, it also includes: (3) placing the obtained fiber film in an oven to dry and remove the solvent to obtain the final sample.

Based on the above technical solution, preferably, the drying temperature is 36-38 degrees Celsius, and the drying time is 22-26 hours.

Based on the above technical scheme, preferably, in step (1), the molecular weight of the polycaprolactone is 80000; the molecular weight of the dimethylglycine is 175.14; the molecular weight of the dimethylformamide is 73.09 ; The molecular weight of the dimethylformamide is 72.11.

The present invention also relates to the protection of the loaded dimethyloxalylglycine nanofiber prepared by the above method.

Electrospun nanofibers are widely used in wound dressings and functional materials due to their biomimetic nanofiber structure, high specific surface area, large volume ratio, and high porosity. Polycaprolactone is a very important synthetic polymer material. Polycaprolactone is a linear polyester with a low melting point. It can be dissolved in various organic solvents to form a solution-type spinning solution, and can be heated to 80-160°C A molten spinning solution is formed. As a biodegradable and biocompatible material, polycaprolactone has been applied in the repair research of many tissues. Dimethyloxalylglycine is a proline hydroxylase inhibitor and an agonist of hypoxia-inducible factor 1α, which can inhibit the degradation of intracellular hypoxia-inducible factor 1α, stabilize the expression of hypoxia-inducible factor 1α, and regulate cell The transcription and expression of internal target genes such as heme oxygenase 1, vascular endothelial growth factor, and erythropoietin are increased, and the expression of inflammatory factors is inhibited to exert their protective effect. The invention utilizes dimethyloxalylglycine to have the function of promoting angiogenesis and wound healing under hypoxic conditions, and is beneficial to treating chronic refractory wounds.

Beneficial effects of the present invention:

The preparation method of the nanofiber loaded with dimethyloxalylglycine provided by the present invention has no similar preparation method in existing products. The present invention combines dimethyloxalylglycine and polycaprolactone nanofibers to prepare a nanofiber material having the advantages of the above two substances, which is an effective therapeutic dressing for chronic ischemic wounds and is beneficial to wound healing , and at the same time, the method is clean, green, efficient and low in cost.

Description of drawings

Fig. 1 is the transmission electron micrograph of the loaded dimethyloxalylglycine nanofiber prepared in embodiment 1;

Fig. 2 is the contact angle of the loaded dimethyloxalylglycine nanofiber film prepared in embodiment 1;

Fig. 3 is the diameter-to-aperture ratio of the loaded dimethyloxalylglycine nanofiber membrane prepared in Example 1.

detailed description

The following non-limiting examples can enable those skilled in the art to understand the present invention more fully, but do not limit the present invention in any way.

Example 1

(1) Take 0.9002g polycaprolactone and 0.3850g dimethyloxalylglycine respectively with a balance, and pour them into a mixed solvent of 3ml dimethylformamide and 1ml tetrahydrofuran;

(2) At room temperature, place it on a magnetic stirrer and stir overnight to obtain a uniform and stable solution;

(3) Pour the configured solution into the needle tube of a 5ml syringe, fix it on the electrospinning device, set the voltage to 8kv, the flow rate to 0.6ml/h, and the rotating speed of the receiving drum to 550r/min, and perform electrospinning to obtain a nanofiber film;

(4) The obtained fiber film was placed in an oven at 37 degrees Celsius, dried for 24 hours, and the solvent was removed to obtain the final sample.

Fig. 1 is the transmission electron micrograph of the loaded dimethyloxalylglycine nanofiber prepared in embodiment 1; As shown in Fig. 1, the resulting product mainly exists in the form of continuous smooth fibers, and the diameters are mostly concentrated in the range of 1-2 μm. Randomly stacked into a non-woven form.

Fig. 2 is the contact angle of the loaded dimethyloxalylglycine nanofiber membrane prepared in Example 1; as shown in Fig. 2, the contact angle of the obtained product is 106.8 degrees.

Fig. 3 is the diameter-to-aperture ratio of the loaded dimethyloxalylglycine nanofiber membrane prepared in Example 1. As shown in Figure 3, 16 positions of the obtained product were randomly selected for comparison of the diameter and the adjacent space (aperture), and the ratio of fiber pore diameter to diameter was uniform.

Claims (10)

1. a preparation method of loading dimethyloxalylglycine nanofiber, is characterized in that, comprises the steps: (1) Add polycaprolactone and dimethyloxalylglycine to a mixed solvent of dimethylformamide and tetrahydrofuran, place it in a magnetic stirrer, and stir for 8-14 hours at a temperature of 20-25 degrees Celsius. hours, to obtain a uniform and stable solution; (2) Electrospinning the configured solution to obtain a nanofiber membrane. 2. the preparation method of loading dimethyloxalylglycine nanofiber according to claim 1 is characterized in that, in step (2), the voltage of electrospinning is 6-10kv, and ejection flow rate is 0.5-0.7 ml/h. 3. the preparation method of load dimethyloxalylglycine nanofiber according to claim 1, is characterized in that, in step (1), the quality of described polycaprolactone and dimethyloxalylglycine The ratio is 0.9002:0.3850. 4. the preparation method of loaded dimethyloxalylglycine nanofiber according to claim 1, is characterized in that, in step (1), the volume ratio of described dimethylformamide and tetrahydrofuran is 3:1. 5. the preparation method of load dimethyloxalylglycine nanofiber according to claim 1 is characterized in that, in step (1), the ratio of the mixed solvent of polycaprolactone and dimethylformamide and tetrahydrofuran It is 0.9002g:4ml. 6. The preparation method of loaded dimethyloxalylglycine nanofibers according to claim 1, characterized in that, in step (2), the rotating speed of the receiving drum is 500-600r/min. 7. The preparation method of loaded dimethyloxalylglycine nanofibers according to claim 1, further comprising: (3) placing the obtained fiber film in an oven to dry to obtain the final sample. 8. The preparation method of loaded dimethyloxalylglycine nanofibers according to claim 1, characterized in that, the drying temperature is 36-38 degrees Celsius, and the drying time is 22-26 hours. 9. The preparation method of loaded dimethyloxalylglycine nanofibers according to claim 1, characterized in that in step (1), the molecular weight of the polycaprolactone is 80,000. 10. The loaded dimethyloxalylglycine nanofiber prepared by the method according to any one of claims 1-9.

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