CN115463039A

CN115463039A - Polypeptide repairing anti-allergy freeze-dried powder and preparation method thereof

Info

Publication number: CN115463039A
Application number: CN202210726545.5A
Authority: CN
Inventors: 陈秀森
Original assignee: Shanghai Hesheng Cosmetic Co ltd
Current assignee: Shanghai Hesheng Cosmetic Co ltd
Priority date: 2022-06-23
Filing date: 2022-06-23
Publication date: 2022-12-13

Abstract

The invention discloses polypeptide repairing anti-allergy freeze-dried powder, which has the advantages that the freeze-dried powder prepared by adding an auxiliary agent, an antioxidant and a skin conditioner into the components of the freeze-dried powder has the effects of moisturizing and repairing, and is good in stability. The freeze-dried powder is strong in activity, can promote synthesis of collagen and elastin, protects neutral mitochondrial cells, improves antioxidant enzyme activity, realizes recovery of self-repairing capacity of skin, increases thickness of skin stratum corneum, enhances skin immune function, improves cell stress capacity, and is beneficial to helping skin recover compactness, softness, smoothness and elasticity. The addition of the polypeptide, the plant extract and the anti-allergy agent in the solvent components enables the solvent to have a powerful transdermal absorption effect on skin, has a fast absorption rate, can inhibit various inflammatory factors, prevents inflammatory reaction from proceeding, and further relieves skin itch. The composition can be used by mixing and shaking uniformly and applying on skin, and the lyophilized powder has the advantages of small dosage, easy mixing, convenient use, and wide application in sensitive skin.

Description

Polypeptide repairing anti-allergy freeze-dried powder and preparation method thereof

Technical Field

The invention relates to the field of preparation of freeze-dried powder, in particular to A61Q19/00, and especially relates to polypeptide repair anti-allergy freeze-dried powder and a preparation method thereof.

Background

Modern people often have inflammation due to irregular diet and work and rest, and the skin has poor tolerance, weak immunity and impaired barrier function, is easily affected by air pollution, ultraviolet irradiation and the like in the environment, causes symptoms of reddish skin, pruritus, repeated acne growth, red blood streak and the like, and further affects the personal spiritual appearance and quality. The freeze-dried powder is prepared by freezing the liquid medicine into a solid state in an aseptic environment and freeze-drying the liquid medicine, so that the biological activity of the substance can be well maintained, the transportation is facilitated, and the storage life of the product is prolonged. The activity of the functional substances contained in the composition can be realized by matching the composition with a specific solvent, so that the composition helps to relieve skin problems, repair damaged skin barriers and relieve anaphylactic reaction.

The patent discloses CN202010129650.1 a nourishing and repairing freeze-dried powder and a preparation method thereof, the nourishing and repairing freeze-dried powder comprises two parts, namely freeze-dried powder and a solvent, wherein the freeze-dried powder comprises mannitol, trehalose, ectoin, mixed peptide, disodium hydrogen phosphate, sodium dihydrogen phosphate and sodium chloride, the solvent comprises hyaluronic acid, polyglutamic acid, oat glucan and hexanediol, the obtained freeze-dried powder can restore the skin repairing capability, compact the skin and reduce fine wrinkles, but the active ingredients are absorbed by a human body at a low speed, the anti-allergy effect of the active ingredients is poor, and the applicability of the active ingredients to people with sensitive skin is low. Patent CN202110953338.9 discloses a preparation method and application of freeze-dried powder with sensitive skin barrier repair effect, wherein the freeze-dried powder comprises mannitol, oligopeptides 1, 3 and 5 and superoxide dismutase MSOD, a solvent comprises kava root and stem extract, centella asiatica, purslane, cactus, radix sophorae flavescentis, radix ophiopogonis extract and p-hydroxyacetophenone, and the repair effect of a single raw material is enhanced by utilizing the synergistic effect of three substances of plant extract, plant fermentation liquor and polypeptide.

Disclosure of Invention

In order to solve the problems, the invention provides a polypeptide repairing anti-allergy freeze-dried powder which comprises a freeze-dried powder component and a solvent component.

In some preferred embodiments, the raw materials for preparing the lyophilized powder components comprise: according to the mass percentage, 7 to 20 percent of auxiliary agent, 0.5 to 5 percent of antioxidant, 0.15 to 3 percent of skin conditioner and water are added to 100 percent.

In some preferred embodiments, the adjuvant is selected from one or more of mannitol, glycerol glucoside, inositol, sucrose, lactose, mannitol, trehalose, dextran, guava, maltose; preferably, the adjuvant is mannitol.

In some preferred embodiments, the antioxidant is selected from one or more of vitamin C, tocopherol, tetrahydromethylpyrimidine carboxylic acid, protein hydrolysates, amino acids, cysteine, lecithin, butylated hydroxyanisole, tert-butylhydroquinone; preferably, the antioxidant is a tetrahydro-methyl-pyrimidine-carboxylic acid.

Tetrahydromethylpyrimidine carboxylic acid, also known as ectoin (Ection), is highly hydrophilic and has long-lasting moisturizing effect. Meanwhile, the collagen peptide plays a chemical transmitter-like role in cells, can enhance the activity of fibroblasts, promote the synthesis of collagen, protect neutrophils, play a role in resisting inflammation, relieving, resisting aging and repairing damaged skin, improve the activity of antioxidase and protect Langerhans cells. In addition, the tetrahydro-methyl pyrimidine carboxylic acid can protect DNA from ultraviolet rays and visible light damage, and has the effect of sun protection.

In some preferred embodiments, the skin conditioning agent is selected from one or more of panthenol, collagen, allantoin, tripeptide-1 copper, hydrolyzed elastin, carnosine, dipotassium glycyrrhizinate, phytosterol oleate; preferably, the skin conditioning agent is the tripeptide-1 copper and carnosine.

Copper is a trace element required for maintaining body functions, has rich and complex functions, is an element required for the action of various cell enzymes, is applied to skin tissues, and has excellent functions of resisting oxidation, promoting collagen proliferation and assisting wound healing. Tripeptide-1 copper consisting of three amino acids and one copper ion can effectively promote the generation of collagen and elastin, make skin compact, fade fine wrinkles, promote angiogenesis, increase oxygen supply, show certain oxidation resistance, make skin tender, white and glossy, and stimulate the generation of glucose polyamine, thereby realizing the recovery of self-repairing capability of the skin, improving the skin stratum corneum and reducing the skin damage and loosening.

In some preferred embodiments, the tripeptide-1 copper to carnosine mass ratio is 1: (0.5-1.5); preferably, the mass ratio of the tripeptide-1 copper to the carnosine is 1:1.

in some preferred embodiments, the vehicle component is prepared from raw materials including: according to the mass percentage, 0.55 to 10.5 percent of humectant, 1.1 to 10 percent of polyol, 0.05 to 1 percent of preservative, 0.02 to 0.5 percent of polypeptide, 0.5 to 7 percent of plant extract, 0.2 to 2 percent of anti-allergy agent and water are added to 100 percent.

In some preferred embodiments, the humectant is selected from one or more of PEG castor oil, hydrolyzed glycosaminoglycans, sodium hyaluronate, glycerin, bis-diethoxydiethylene glycol cyclohexane, xylitol, sorbitol; preferably, the humectants are sodium hyaluronate and glycerin.

In some preferred embodiments, the mass ratio of sodium hyaluronate to glycerin is (0.05-0.5): (0.5 to 10); preferably, the mass ratio of the sodium hyaluronate to the glycerol is 0.15:5.

in some preferred embodiments, the polyol is selected from one or more of propylene glycol, glycerol, 1,2-hexanediol, 1,2-butanediol, polyoxyethylene sorbitol, pentanediol, ethylhexylglycerin; preferably, the polyol is 1,2-hexanediol, 1,2-butanediol.

In some preferred embodiments, the 1,2-hexanediol and 1,2-butanediol are present in a mass ratio of 1: (0.8-2); preferably, the mass ratio of 1,2-hexanediol to 1,2-butanediol is 1:1.

in some preferred embodiments, the preservative is selected from one or more of parabens, p-hydroxyacetophenone, imidazolidinyl urea, caprylyl hydroxamic acid, phenoxyethanol, benzoic acid, ethylparaben solutions; preferably, the preservative is p-hydroxyacetophenone.

In some preferred embodiments, the polypeptide is selected from one or more of acetyl tetrapeptide-2, fibronectin, palmitoyl tripeptide-1, hexapeptide-2, palmitoyl tetrapeptide-7, acetyl octapeptide-3, acetyl hexapeptide-8, palmitoyl pentapeptide-4, mung bean peptide, trehal firming peptide; preferably, the polypeptide is fibronectin.

In some preferred embodiments, the molecular weight of fibronectin is 30000-45000 Da; preferably, the molecular weight of fibronectin is 40000Da.

In some preferred embodiments, the plant extract is selected from one or more of Glycyrrhiza Glabra (Glycyrrhiza Glabra) root extract, seaweed (Sargassum fusiforme (harv.) switch) extract, centella Asiatica (Centella Asiatica) extract, polygonum Cuspidatum (Polygonum Cuspidatum) root extract, butcher's broom (Ruscus Aculeatus) root extract, scutellaria Baicalensis (Scutellaria Baicalensis) root extract, ginseng (Panax Ginseng) root extract, chrysanthemum (chrysanthlemum Morifolium) extract, tremella Fuciformis (Tremella Fuciformis) fruit body extract, crocus sativus (Crocus sativus l.) extract; preferably, the plant extracts are glycyrrhiza glabra root extract and centella asiatica extract.

In some preferred embodiments, the Glycyrrhiza Glabra root extract is a mixture of 1,2-1,2-butanediol, water, centella Asiatica (Centella Asiatica) extract, polygonum Cuspidatum (Polygonum Cuspidatum) root extract, scutellaria Baicalensis (Scutellaria baicaliensis) root extract, camellia Sinensis (Camellia Sinensis) leaf extract, glycyrrhiza Glabra (Glycyrrhiza Glabra) root extract, chamomila (Chamomila Recutita) flower extract, rosmarinus Officinalis (Rosmarinus Officinalis) leaf extract).

The glycyrrhiza glabra root extract in the application can comprehensively inhibit various inflammatory factors, quickly prevent quick chain physiological reaction of stage inflammation, relieve skin itch, diminish inflammation, resist allergy and pain, inhibit the damage of inflammatory mediators to normal cells, achieve the effects of soothing allergy, resisting allergy, diminishing inflammation and removing redness and simultaneously has the synergistic effects of resisting oxidation and moisturizing under the synergistic effect of combining seven plant extracts.

The centella asiatica extract selected in the application contains an alpha-resinol structure, and the main components of the centella asiatica extract are asiaticoside and madecassoside. Centella asiatica extract can tighten the portion of the epidermis that connects to the dermis, and also helps promote collagen formation in the dermis, making the skin tight, soft, smooth and elastic, and lightening fine lines and wrinkles. But also can help the injured tissue to heal, promote the close connection between the epidermis and the dermis, inhibit the increase of fat cells, and reduce the problems of skin edema, obesity and the like. In the application, the glycyrrhiza glabra root extract with a specific composition and the centella asiatica extract are mixed for use, under the coordination of other substances in a solvent component, the solubility is good, the stability is strong, the activity of the plant extract is high, the effect is obvious, the influence of freeze drying on the activity of the plant extract is avoided, when the plant extract is mixed with a freeze-dried powder component for use in the using process, the dissolving rate is high, the plant extract and the components of carnosine, tetrahydro-methylpyrimidine carboxylic acid, tripeptide-1 copper and the like are cooperated for acting, the anti-allergy and repair effects are jointly achieved, and the effect is fast.

In some preferred embodiments, the mass ratio of the glycyrrhiza glabra root extract to the centella asiatica extract is (0.5-5): (0.2 to 2); preferably, the mass ratio of the glycyrrhiza glabra root extract to the centella asiatica extract is 2:1.

in some preferred embodiments, the anti-sensitivity agent is selected from one or more of allantoin, gentian anti-irritant factor, dipotassium glycyrrhizinate, yarrow (Achillea alpina l.) extract, bisabolol, tea tree essential oil; preferably, the anti-sensitivity agent is gentian anti-irritant factor.

The second aspect of the invention provides a preparation process of polypeptide repair anti-allergy freeze-dried powder, which comprises the following steps:

(1) Preparing freeze-dried powder components;

(2) Preparation of solvent component.

In some preferred embodiments, the step (1) of preparing the lyophilized powder component specifically comprises the following steps:

s1, heating water to 100-120 ℃, and preserving heat for 20-50 min;

s2, cooling the water in the S1 to 35-60 ℃, sequentially adding the auxiliary agent, the antioxidant and the skin conditioner, and uniformly stirring to obtain a mixture.

And S3, taking the mixture sample obtained in the S2, detecting, filling the mixture sample into a 5mL penicillin bottle after the mixture sample is qualified, and covering a bottle stopper as required.

And S4, sequentially putting the penicillin bottles obtained in the step S3 into a freeze dryer, setting a freeze-drying temperature program, and starting freeze-drying.

S5, after the freeze-drying is finished, the inner plug is compressed, the penicillin bottle is taken out, and the gland is pressed, so that the components of the freeze-dried powder are obtained.

In some preferred embodiments, the lyophilization temperature program in S4 is: the first step is as follows: freezing at-35-45 ℃ for 3-4 h; the second step is that: vacuumizing until the vacuum degree is 30-50 mPa and maintaining for 0.8-1.5 h; the third step: maintaining the temperature at-25 to-32 ℃ for 0.8 to 1.5 hours; the fourth step: maintaining the temperature for 3.5 to 4.5 hours at the temperature of between 5 ℃ below zero and 15 ℃ below zero; the fifth step: maintaining the temperature at 5-15 ℃ for 3.5-4.5 h; and a sixth step: maintaining the temperature at 30-40 ℃ for 7.5-8.5 h.

The applicant can avoid the change of the form and structure of the components of the freeze-dried powder by setting the temperature of the freeze-drying program, thereby ensuring the efficacy performance and the storage stability of the freeze-dried powder. However, the applicant finds that the freeze drying process, particularly the setting of the vacuum degree in the freeze drying process, has obvious influence on the forming and pore structure of the components of the obtained freeze-dried powder. Under a specific temperature and time program, when the vacuum degree is set to be 30-50 mPa, the obtained freeze-dried powder component has uniform color, is easy to form and has compact pores, but when the vacuum degree is lower than 30mPa, obvious pores are formed on the surface of the freeze-dried powder component, and when the vacuum degree is higher than 50mPa, the freeze-dried powder component is found to be loose and difficult to form. It is presumed that under such temperature and time conditions, the surface layer of the frozen material is first dehydrated to form a dry shell having a dense structure, but when the vacuum pressure is low, the water molecules of the lower layer material have a high resistance to passage during sublimation, and the water molecules are accumulated on the dry surface layer and retained for a long time, so that the dried material is secondarily deliquesced, and the volume shrinkage and the appearance are not full. However, when the vacuum degree is too high, the components of the freeze-dried powder are scattered along with water molecules, the structure is loose, the components are further dissipated after the vacuum is released, and the formability is reduced.

Preferably, the lyophilization temperature program in S4 is: the first step is as follows: freezing at-40 deg.C for 4h; the second step is that: vacuumizing until the vacuum degree is 40mPa and maintaining for 1h; the third step: maintaining at-30 deg.C for 1h; the fourth step: maintaining at-10 deg.C for 4h; the fifth step: maintaining at 10 deg.C for 4h; and a sixth step: maintained at 35 ℃ for 8h.

In some preferred embodiments, the preparation of the vehicle component in step (2) specifically comprises the following steps:

s1, sequentially adding water, a humectant and a preservative into an emulsifying pot, starting stirring, heating to 70-95 ℃, and dissolving;

s2, when the temperature of the mixture obtained in the S1 is reduced to 45-50 ℃, the polypeptide, the plant extract and the anti-allergy agent are sequentially added and stirred uniformly.

And S3, when the temperature of the mixture obtained in the S2 is reduced to 30-40 ℃, filtering and discharging by using a filter cloth of 100-300 meshes.

Compared with the prior art, the invention has the following beneficial effects:

(1) The addition of the auxiliary agent, the antioxidant and the skin conditioner in the freeze-dried powder component enables the prepared freeze-dried powder component to have the effects of moisturizing and repairing, and the stability is good. The freeze-dried powder has strong component activity, can promote the synthesis of collagen and elastin, protect neutral mitochondrial cells, improve the activity of antioxidant enzyme, realize the recovery of the self-repairing capacity of skin, increase the thickness of the horny layer of the skin, enhance the immune function of the skin, resist external pressure, improve the stress capacity of cells, and is beneficial to helping the skin to recover compactness, softness, smoothness and elasticity.

(2) The addition of the polypeptide, the plant extract and the anti-allergy agent in the solvent components in the application enables the solvent to have a powerful transdermal absorption effect on skin, is high in absorption rate, can inhibit various inflammatory factors, prevents inflammatory reaction from proceeding, further relieves skin itch, induces regeneration of cells of various nutrient substances, really solves the symptoms of skin water deficiency, aging and sensitivity from a cell layer, and achieves the effects of moisturizing, allergy relieving, inflammation diminishing and redness removing. And through adding multiple plant extracts in the solvent component, the efficacy activity of the freeze-dried powder is effectively maintained, the dosage of nutrient substances is reduced, and through the synergistic effect of multiple components, the stability is good, and meanwhile, the freeze-dried powder is quickly dissolved, so that the obtained freeze-dried powder solution is transparent and stable.

(3) The freeze-dried powder solution in the application is a double-dosage form, and the freeze-dried powder solution is mixed and shaken uniformly to be smeared on skin during use, so that the use amount of the freeze-dried powder is less, the freeze-dried powder is easy to mix, the use is convenient, and the freeze-dried powder can be widely used for sensitive skin.

Detailed Description

Example 1:

1. a polypeptide repairing anti-allergy lyophilized powder comprises lyophilized powder component and solvent component.

The freeze-dried powder comprises the following raw materials: according to the mass percentage, 12% of auxiliary agent, 3% of antioxidant, 1% of skin conditioner and water are added to 100%.

The auxiliary agent is mannitol.

The antioxidant is tetrahydromethylpyrimidine carboxylic acid (Hua Xi biosome).

The skin conditioner is tripeptide-1 copper (Zhejiang surge peptide Biotechnology, inc.) and carnosine (Zhejiang surge peptide Biotechnology, inc.).

The mass ratio of the tripeptide-1 copper to the carnosine is 1:1.

the preparation raw materials of the solvent component comprise: according to the mass percentage, 5.15 percent of humectant, 6 percent of polyalcohol, 0.5 percent of preservative, 0.2 percent of polypeptide, 3 percent of plant extract, 1 percent of anti-allergy agent and water are added to 100 percent.

The humectant is sodium hyaluronate (Hua Xi biology) and glycerin.

The mass ratio of the sodium hyaluronate to the glycerin is 0.15:5.

the polyol was 1,2-hexanediol, 1,2-butanediol (available from DAICEL CORPORATION).

The mass ratio of 1,2-hexanediol to 1,2-butanediol is 1:1.

the preservative is p-hydroxyacetophenone.

The polypeptide is fibronectin.

The molecular weight of fibronectin is 40000Da (Shanghai Tuyuan New Material Co., ltd.).

The plant extracts are Glycyrrhiza glabra root extract and centella asiatica extract (Shanghai Gajia leaf industry Co., ltd.).

The Glycyrrhiza Glabra root extract is a mixture of butylene glycol, water, centella Asiatica (Centella Asiatica) extract, polygonum Cuspidatum (Polygonum Cuspidatum) root extract, scutellaria Baicalensis (Scutellaria Baicalensis) root extract, camellia Sinensis (Camellia Sinensis) leaf extract, glycyrrhiza Glabra (Glycyrrhiza Glabra) root extract, matricaria Chamomilla (Chamomilla Recutita) flower extract, and Rosmarinus Officinalis (Rosmarinus Officinalis) leaf extract (Calyang, chemical engineering (China)).

The mass ratio of the glycyrrhiza glabra root extract to the centella asiatica extract is 2:1.

the anti-allergy agent is gentian anti-irritant factor (shanghai furui trade ltd).

2. A preparation process of polypeptide repairing anti-allergy freeze-dried powder comprises the following steps:

(1) Preparing freeze-dried powder components;

(2) Preparation of solvent component.

The preparation of the freeze-dried powder component in the step (1) comprises the following steps:

s1, heating water to 120 ℃, and preserving heat for 30min;

s2, cooling the water in the S1 to 45 ℃, sequentially adding the auxiliary agent, the antioxidant and the skin conditioner, and uniformly stirring to obtain a mixture.

And S3, taking the mixture sample obtained in the step S2, detecting, filling the mixture sample into a 5mL penicillin bottle after the mixture sample is qualified, and covering the bottle stopper as required.

The freeze-drying temperature program in the S4 is as follows: the first step is as follows: freezing at-40 deg.C for 4h; the second step: vacuumizing until the vacuum degree is 40mPa and maintaining for 1h; the third step: maintaining at-30 deg.C for 1h; the fourth step: maintaining at-10 deg.C for 4h; the fifth step: maintaining at 10 deg.C for 4h; and a sixth step: maintained at 35 ℃ for 8h.

The preparation of the solvent component in the step (2) comprises the following steps:

s1, sequentially adding water, a humectant and a preservative into an emulsifying pot, starting stirring, heating to 85 ℃, and dissolving;

s2, when the temperature of the mixture obtained in the S1 is reduced to 45 ℃, the polypeptide, the plant extract and the anti-allergy agent are sequentially added and stirred uniformly.

And S3, when the temperature of the mixture obtained in the S2 is reduced to 40 ℃, filtering and discharging by using a filter cloth of 200 meshes.

Example 2:

1. a polypeptide repairing anti-allergy freeze-dried powder is different from the freeze-dried powder in example 1 in that:

the freeze-drying temperature program in the S4 is as follows: the first step is as follows: freezing at-40 deg.C for 4h; the second step is that: vacuumizing until the vacuum degree is 45mPa and maintaining for 1h; the third step: maintaining at-30 deg.C for 1h; the fourth step: maintaining at-10 deg.C for 4h; the fifth step: maintaining at 10 deg.C for 4h; and a sixth step: maintained at 35 ℃ for 8h.

2. A method for preparing polypeptide repairing anti-allergy freeze-dried powder, which is the same as the example 1.

Comparative example 1:

1. the difference between the polypeptide repair anti-allergy freeze-dried powder and the freeze-dried powder in the embodiment 1 is as follows:

the lyophilization temperature program in the S4 comprises the following steps: the first step is as follows: freezing at-40 deg.C for 4h; the second step is that: vacuumizing until the vacuum degree is 60mPa and maintaining for 1h; the third step: maintaining at-30 deg.C for 1h; the fourth step: maintaining at-10 deg.C for 4h; the fifth step: maintaining at 10 deg.C for 4h; and a sixth step: maintained at 35 ℃ for 8h.

Comparative example 2:

the plant extract is glycyrrhiza glabra root extract.

The Glycyrrhiza Glabra root extract is a mixture of 1,2-1,2-butanediol, water, centella Asiatica (Centella Asiatica) extract, polygonum Cuspidatum (Polygonum Cuspidatum) root extract, scutellaria Baicalensis (Scutellaria Baicalensis) root extract, camellia Sinensis (Camellia Sinensis) leaf extract, glycyrrhiza Glabra (Glycyrrhiza Glabra) root extract, chamomilla Recutita (Chamomilla Recutita) flower extract, and Rosmarinus Officinalis (Rosmarinus Officinalis Officnalis) leaf extract (Calyan, co., ltd., korea).

The palmitoyl tripeptide-1 is purchased from Xianxinlu Biotechnology Limited.

And (3) performance testing:

1. physical and chemical properties: the lyophilized powder obtained in example 1 was tested for its composition and solvent content according to the standards of Shanghai and cosmetics Co., ltd.

TABLE 1 sanitation index

TABLE 2 sensory indices

2. Cold-hot cycle stability: the lyophilized powder and solvent obtained in examples and comparative examples were circulated at low temperature (-8 + -2) ° C, 40 + -1) ° C and high temperature for 3 times, each circulation was maintained for 24h, the low temperature was maintained for 12h, and the state of the essence before and after circulation was observed by eye. After the temperature is restored to the room temperature, no obvious character difference with that before the test is recorded as A; a small turbidity was observed as B and a partial turbidity was observed and even a precipitate appeared as C.

3. Solubility: 2g (before freeze-drying) of the freeze-dried powder components obtained in the examples and the comparative examples and 5g of the solvent components are dissolved, and the appearance of the solution is observed.

	Example 1	Example 2	Comparative example 1	Comparative example 2
					Lyophilized powder component	Class A	Class A	Class C	Class A
Solvent component	Class A	Class A	Class A	Class B
					Solubility in water	Is transparent and uniform	Is transparent and uniform	Is opaque and non-uniform	Is opaque and non-uniform

4. And (3) testing the efficacy: a using sample is prepared by mixing 2g (before freeze-drying) freeze-dried powder component obtained in example 1 with 5g of solvent component, 20 females between 30 and 50 years old are selected, the testing time is one week, an experimenter only uses the sample without other skin care products within one week, the experimenter uses the sample once in the morning and at night, and the smearing range is the whole face. And (5) judging a result: the sense, feeling and vision of the experimenter are used for judgment.

Serial number	Product effect	Number of people
			1	Feeling the facial skin to have obvious firming feeling	11
2	The sense of fine lines on the skin is obviously improved	5
			3	Feel more elastic to the skin	2
4	Feel more water tender	2

Claims

1. The polypeptide repairing anti-allergy freeze-dried powder is characterized by comprising the following components in parts by weight:

the freeze-dried powder comprises the following raw materials: according to the mass percentage, 7 to 20 percent of auxiliary agent, 0.5 to 5 percent of antioxidant, 0.15 to 3 percent of skin conditioner and water are added to 100 percent;

the preparation raw materials of the solvent component comprise: according to the mass percentage, 0.55 to 10.5 percent of humectant, 1.1 to 10 percent of polyatomic alcohol, 0.05 to 1 percent of preservative, 0.02 to 0.5 percent of polypeptide, 0.5 to 7 percent of plant extract, 0.2 to 2 percent of anti-allergic agent and water are added to 100 percent.

2. The polypeptide-repairing anti-allergy lyophilized powder of claim 1, wherein the adjuvant is one or more selected from mannitol, glycerol glucoside, inositol, sucrose, lactose, mannitol, trehalose, dextran, gellan gum and maltose.

3. The polypeptide-repairing anti-allergy lyophilized powder of claim 1, wherein the antioxidant is selected from one or more of vitamin C, tocopherol, tetrahydro-methyl-pyrimidine-carboxylic acid, protein hydrolysate, amino acid, cysteine, lecithin, butyl hydroxy anisole, and tert-butyl hydroquinone.

4. The polypeptide-repairing anti-allergy freeze-dried powder of claim 1 or 3, wherein the antioxidant is tetrahydro-methyl-pyrimidine-carboxylic acid.

5. The polypeptide-repairing, anti-allergy lyophilized powder of claim 1, wherein said skin conditioning agent is selected from one or more of panthenol, collagen, allantoin, tripeptide-1 copper, hydrolyzed elastin, carnosine, dipotassium glycyrrhizinate, and phytosterol oleate.

6. The polypeptide-repairing antiallergic lyophilized powder of claim 1, wherein the polyol is one or more selected from the group consisting of propylene glycol, glycerol, 1,2-hexanediol, 1,1,2-butanediol, polyoxyethylene sorbitol, pentanediol, and ethylhexylglycerin.

7. The polypeptide-repairing antiallergic lyophilized powder of claim 1, wherein the polypeptide is selected from one or more of acetyl tetrapeptide-2, fibronectin, palmitoyl tripeptide-1, hexapeptide-2, palmitoyl tetrapeptide-7, acetyl octapeptide-3, acetyl hexapeptide-8, palmitoyl pentapeptide-4, mung bean peptide, and algal firming peptide.

8. The polypeptide-repairing anti-allergy freeze-dried powder of claim 1, wherein the plant extract is one or more selected from a group consisting of a glycyrrhiza glabra root extract, a seaweed extract, a centella asiatica extract, a polygonum cuspidatum root extract, a butea frondosa root extract, a scutellaria baicalensis root extract, a ginseng root extract, a chrysanthemum extract, a tremella fuciformis fruiting body extract and a saffron extract.

9. The polypeptide repair anti-allergy lyophilized powder of claim 1, wherein the anti-allergy agent is selected from one or more of allantoin, gentian anti-irritant factor, dipotassium glycyrrhizinate, achillea L extract, bisabolol, and tea tree essential oil.

10. A method for preparing polypeptide repair anti-allergy freeze-dried powder according to any one of claims 1 to 9, which comprises the following steps:

(1) Preparing freeze-dried powder components;

(2) Preparing a solvent component;

s1, heating water to 100-120 ℃, and preserving heat for 20-50 min;

s2, cooling the water in the S1 to 35-60 ℃, sequentially adding the auxiliary agent, the antioxidant and the skin conditioner, and uniformly stirring to obtain a mixture;

s3, detecting the mixture sample obtained in the step S2, filling the mixture sample into a 5mL penicillin bottle after the mixture sample is qualified, and covering a bottle stopper according to requirements;

s4, sequentially putting the penicillin bottles obtained in the S3 into a freeze dryer, setting a freeze-drying temperature program, and starting freeze-drying;