CN115444142A

CN115444142A - Preparation method and application of composite prebiotics

Info

Publication number: CN115444142A
Application number: CN202211232127.7A
Authority: CN
Inventors: 刘学志; 张�成
Original assignee: Hongzhi Guangzhou Biotechnology Co ltd
Current assignee: Hongzhi Guangzhou Biotechnology Co ltd
Priority date: 2022-10-08
Filing date: 2022-10-08
Publication date: 2022-12-09

Abstract

The invention discloses a preparation method and application of composite prebiotics, wherein fructo-oligosaccharide can proliferate beneficial bacteria in vivo, but the types of the beneficial bacteria proliferated by oligosaccharide of each component are different. The sucrose FOS can be proliferated by beneficial bacteria of the following types: the total number of the bifidobacterium adolescentis, the bifidobacterium infantis, the bifidobacterium longum, the bifidobacterium breve, the lactobacillus acidophilus, the lactobacillus plantarum and the lactobacillus salivarius is 7, wherein GF2 can fully proliferate the 7 beneficial bacteria, and GF2 is the most excellent component in sucrose FOS. The chain length of the chicory fructo-oligosaccharide ranges from 2 to 8, and the wide polymerization degree range can bring about a more durable proliferation process. Wherein, the 6-sugar, the 7-sugar and the 8-sugar have higher polymerization degree, so that beneficial bacteria in intestinal tracts can not be directly utilized, the beneficial bacteria at the tail segment in the colon can also obtain a nutrient source to be massively proliferated, and short-chain fatty acid generated by the metabolism of the beneficial bacteria can supplement energy required by the proliferation of epithelial cells of the segment of colon.

Description

Preparation method and application of composite prebiotics

Technical Field

The invention relates to the technical field of prebiotics, in particular to a preparation method and application of composite prebiotics.

Background

Prebiotics are ingredients that can be selectively fermented to specifically alter the composition and activity of the beneficial microflora in the gut to the health of the host, and can remain intact through the digestive tract into the large intestine. Prebiotics ingested from the external environment can be metabolized by bacteria with selective transporters and grow on the carbohydrates. Researches show that the prebiotics can influence intestinal flora, change stool habits, interact with absorbed mineral substances, interact with cell proliferation and peripheral anticancer tissues in the intestinal tract, regulate immune function, endocrine activity, fat metabolism and the like, and are very beneficial to human bodies.

Fructo-oligosaccharide is a typical prebiotic, has the effects of proliferating intestinal beneficial bacteria, balancing human microecology, relaxing bowel, improving organism immunity and the like, and is widely applied to health food and food industries: such as infant formula, dairy products, beverages, baked foods and the like.

There are two sources of fructooligosaccharides used in industry: fructo-oligosaccharide produced by using sucrose as a raw material and fructo-oligosaccharide produced by using chicory as a raw material. Wherein the polymerization degree of fructo-oligosaccharide from sucrose is 3-5, and the structural formula is represented as GFn (G is glucosyl, F is fructosyl, and n = 2-4). The sucrose FOS comprises the following components: the fructotriose GF2 with the polymerization degree of 3, the fructotetraose GF3 with the polymerization degree of 4, the fructopentaose GF4 with the polymerization degree of 5 and the molecular weight of 504-828, wherein the fructotriose GF2 component of fructo-oligosaccharide can be quickly utilized by beneficial bacteria in the large intestine of a human body due to low polymerization degree, and can quickly proliferate the beneficial bacteria in the large intestine, but intestinal side effects such as flatulence, laxation and the like easily caused by quick fermentation of micromolecule GF2 with low polymerization degree are great obstacles to the application of foods for infants and children. The polymerization degree of fructo-oligosaccharide from chicory is 2-8, and the fructo-oligosaccharide comprises two structural formulas, wherein one structural formula is GFn (G is glucosyl, F is fructosyl, n = 2-7), and the other structural formula is Fn (F is fructosyl, n = 2-8).

In conclusion, the single sucrose fructo-oligosaccharide or chicory fructo-oligosaccharide has some defects, and a fructo-oligosaccharide prebiotic with all-round excellent effects needs to be developed to meet the demand.

Therefore, the person skilled in the art provides a preparation method and application of complex prebiotics to solve the problems in the background art.

Disclosure of Invention

The invention aims to provide a preparation method and application of a composite prebiotic to solve the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme:

the preparation method and the application of the composite prebiotics are characterized by comprising sucrose fructo-oligosaccharide and chicory fructo-oligosaccharide, wherein the weight ratio of the sucrose fructo-oligosaccharide to the chicory fructo-oligosaccharide is 1.

As a further scheme of the invention: the weight ratio of the sucrose fructo-oligosaccharide to the chicory fructo-oligosaccharide is 1.

As a still further scheme of the invention: the sucrose fructo-oligosaccharide contains kestose GF2 accounting for more than 70 percent of the pure sucrose fructo-oligosaccharide, and the sum of the contents of nystose GF3 and nystose GF4 accounting for less than 30 percent of the pure sucrose fructo-oligosaccharide.

As a still further scheme of the invention: in the chicory fructo-oligosaccharide, the sum of the contents of heptafructose F7, heptasucrose GF6, hexafructose F6 and hexasucrose GF5 accounts for more than 20 percent of the pure chicory fructo-oligosaccharide, and the sum of the contents of fructo-disaccharide F2, fructo triose F3, kestose GF2, tetrafructose F4, tetrafructose GF3, pentasaccharide F5 and pentasaccharide GF4 accounts for less than 80 percent of the pure chicory fructo-oligosaccharide.

A method for preparing fructo-oligosaccharide compound prebiotics comprises mixing sucrose fructo-oligosaccharide and herba Cichorii fructo-oligosaccharide at a certain proportion at room temperature, dissolving, filtering, sterilizing, and concentrating.

An application of fructo-oligosaccharide compound prebiotics in food or health product is provided.

A fructo-oligosaccharide compound prebiotics preparation is prepared by taking the fructo-oligosaccharide compound prebiotics as active ingredients and is suitable for oral administration.

As a still further scheme of the invention: the fructo-oligosaccharide compound prebiotics preparation is in a paste or powder shape.

Compared with the prior art, the invention has the beneficial effects that:

the fructo-oligosaccharide can proliferate beneficial bacteria in vivo, but the oligosaccharide of each component can proliferate beneficial bacteria in different types. The beneficial bacteria capable of being proliferated by the sucrose FOS comprise the following beneficial bacteria: the total number of the bifidobacterium adolescentis, the bifidobacterium infantis, the bifidobacterium longum, the bifidobacterium breve, the lactobacillus acidophilus, the lactobacillus plantarum and the lactobacillus salivarius is 7, wherein GF2 can fully proliferate the 7 beneficial bacteria, and GF2 is the most excellent component in sucrose FOS. The chain length range of the chicory fructo-oligosaccharide is 2-8, the polymerization degree range is wide, and a more durable proliferation process can be brought. Wherein, the 6-sugar, the 7-sugar and the 8-sugar have higher polymerization degree, so that beneficial bacteria in intestinal tracts can not be directly utilized, the beneficial bacteria at the tail end of colon can also obtain a nutrient source to be massively proliferated, and short-chain fatty acid generated by the metabolism of the beneficial bacteria can supplement energy required by the proliferation of the colon epithelial cells at the segment. However, chicory fructooligosaccharides have less GF 2. The sucrose fructo-oligosaccharide rich in GF2 is compounded with the chicory fructo-oligosaccharide rich in 6 sugar and 7 sugar to produce the compound fructo-oligosaccharide with all-round excellent effect,

the product has the following advantages: (1) The product is rich in GF2 content, and can comprehensively and rapidly proliferate 7 beneficial bacteria; (2) The product has reasonable GF2 content, and can reduce the side effects of flatulence, laxation and the like caused by excessively high fermentation speed; (3) The solubility and the palatability of the product are greatly improved, and the experience of consumers is improved; (4) The product has rich sugar 6 and 7, and can proliferate beneficial bacteria in colon.

Drawings

Fig. 1 is a schematic diagram of detection data and compounding results of a preparation method of a composite prebiotic and application thereof.

Detailed Description

The technical means, the creation characteristics, the achievement purposes and the effects of the invention are easy to understand.

Example 1

As shown in figure 1, the preparation method and the application of the composite prebiotics provided by the invention comprise sucrose fructo-oligosaccharide and chicory fructo-oligosaccharide, wherein chicory FOS is dissolved into a DS25% solution by warm water at 40 ℃, and then heated to evaporate water to DS75%. Taking a sucrose FOS slurry product (solid content DS 75%), and mixing the sucrose FOS syrup and the chicory FOS syrup according to the weight ratio of 1:3, dissolving, filtering, sterilizing and concentrating to prepare the composite GFO fructo-oligosaccharide (DS 75%).

Example 2

Chicory FOS was dissolved in warm water at 40 ℃ to a DS25% solution and then heated to evaporate water to DS75%. Taking a sucrose FOS slurry product (solid content DS 75%), and mixing the sucrose FOS syrup and the chicory FOS syrup according to the weight ratio of 1:2, dissolving, filtering, sterilizing, concentrating and spray drying to obtain the composite GFO fructo-oligosaccharide (the water content is less than or equal to 5%).

Example 3

Chicory FOS was dissolved in warm water at 40 ℃ to a DS25% solution and then heated to evaporate water to DS75%. Taking a sucrose FOS slurry product (solid content DS 75%), and mixing the sucrose FOS syrup and the chicory FOS syrup according to the weight ratio of 1:4, dissolving, filtering, sterilizing and concentrating to prepare the composite GFO fructo-oligosaccharide (DS 75%).

Example 4

Chicory FOS was dissolved in warm water at 40 ℃ to a DS25% solution and then heated to evaporate water to DS75%. Taking a sucrose FOS slurry product (solid content DS 75%), and mixing the sucrose FOS syrup and the chicory FOS syrup according to the weight ratio of 1:5, dissolving, filtering, sterilizing, concentrating and spray drying to obtain the composite GFO fructo-oligosaccharide (the water content is less than or equal to 5%).

Experimental example 1

The content of each component was measured by an ICS-500 ion chromatograph equipped with a quaternary gradient pump and a pulsed amperometric detector using the complex GFO fructo-oligosaccharide (DS 75%) prepared in example 1.

Experimental example 2

The efficacy of the present invention is demonstrated by clinical experimental data below.

Volunteers 1:28 year old female, no history, dietary habit: 1 cup of black tea in 1 day, light, cold constitution, no history of allergy; the product of the embodiment 1 of the invention is continuously taken for 3 days, 20mL of the product is taken at one time, and the taking modes are direct taking/mixing into breakfast porridge respectively; the administration time is morning fasting/breakfast; no reaction in the taking period, and the body feeling is better than that before taking.

Volunteers 2: 36-year-old normal women, dietary habits: cantonese, occasional eating of peppery food, hot constitution and no history of allergy; the product of example 1 of the invention was taken continuously for 5 days, and 10mL of the invention was taken with hot water every day; the administration time is after meal/morning fasting/after meal/breakfast respectively; the reaction condition of every day in the taking period is that the patient exhausts or occasionally exhausts, and defecates once or not, the mouth and tongue have dry feeling or the mouth and tongue have dry feeling; the product has good taste and moderate sweetness.

Volunteers 3: a28-year-old female has history of halitosis and constipation, cold constitution and no allergic history; the product of the embodiment 1 of the invention is continuously taken for 5 days, the invention is taken by 10/20/20/10/10mL each day, and the taking modes are respectively hot water taking/other/hot water taking/other; the daily reaction condition in the taking period is borborygmus exhaust/no/exhaust defecation; the product is thick in taste and texture and is relatively popular.

Volunteers 4:38 year old female with a history of constipation, dietary habits: much eating, hot constitution and no allergic history; the product of the embodiment 1 of the invention is continuously taken for 5 days, the invention is respectively taken for 20/10/10/10/10mL once a day, and the taking modes are respectively direct taking/hot water taking/direct taking; the reaction condition of each day in the taking period is slight exhaust; the sweetness is suitable and acceptable.

Volunteers 5:27 year old women with a history of diarrhea and irritable bowel syndrome, dietary habits: bland, cold constitution with phlegm-dampness, with a history of allergy; after the product of the embodiment 1 of the invention is continuously taken for 6 days, the reaction conditions of dry mouth/borborygmus, dry mouth, increased water intake, warmer hands and feet, dry mouth, long life, exhaustion/acne and early-menstrual lower abdomen cold pain are respectively relieved; the mouthfeel of the invention is acceptable.

Volunteers 6: 37-year-old normal women, dietary habits: the people often eat spicy food, the constitution is cold, and no allergic history exists; the product of the invention in the example 1 is taken continuously for 6 days, and 10mL of the product is taken with hot water at 2 pm every day; the reaction condition of each day in the taking period is none/no/micro abdominal distension/no respectively; the taste of the invention is better than that before taking, and the taste of the invention is acceptable, and the smell of flushing water is more comfortable.

Volunteers 7:29 years old women have history of constipation and acute gastroenteritis, have a slightly hot constitution and no allergic history; the product of the embodiment 1 of the invention is continuously taken for 2 days and 2 times a day, 10/10/20/20mL of the invention is respectively taken with hot water each time, the taking time is respectively after breakfast satiety/empty stomach in the morning, and the reaction condition of each day in the taking period is no/little excessive internal heat/no; the taste of the product was perceived as normal.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. The preparation method of the composite prebiotics is characterized by comprising sucrose fructo-oligosaccharide and chicory fructo-oligosaccharide, wherein the weight ratio of the sucrose fructo-oligosaccharide to the chicory fructo-oligosaccharide is 2-5.

2. The preparation method and the application of the composite prebiotics of claim 1 are characterized in that the weight ratio of the sucrose oligofructose to the chicory oligofructose is 2.

3. The method for preparing composite prebiotics according to any of claims 1 or 2, wherein the sucrose oligofructose has a content of kestose GF2 of more than 70% of the pure sucrose oligofructose and the sum of the contents of nystose GF3 and kestose GF4 of less than 30% of the pure sucrose oligofructose.

4. The method for preparing complex prebiotics according to any of claims 1 or 2, wherein the total content of fructoheptaose F7, heptaose GF6, hexaose F6 and hexaose GF5 in the chicory fructo-oligosaccharides accounts for more than 20% of the pure chicory fructo-oligosaccharides, and the total content of fructodisaccharide F2, fructotriose F3, kestose GF2, tetraose F4, tetraose GF3, pentaose F5 and pentaose GF4 accounts for less than 80% of the pure chicory fructo-oligosaccharides.

5. A preparation method for preparing the composite prebiotics of claim 1 is characterized in that the prebiotics is prepared by mixing sucrose fructo-oligosaccharide and chicory fructo-oligosaccharide in proportion at room temperature, dissolving, filtering, sterilizing and concentrating.

6. The method for preparing the composite prebiotic of claim 1, which is applied to food or health care products.

7. A fructooligosaccharide complex prebiotic formulation characterised in that it is formulated for oral administration using a fructooligosaccharide complex prebiotic according to any one of claims 1 to 4 as active ingredient.

8. The complex fructooligosaccharide prebiotic formulation of claim 7 wherein the complex fructooligosaccharide prebiotic formulation is in the form of a syrup or powder.