CN115436617A - Dry analytical reagent for gamma-glutamyl transpeptidase detection - Google Patents

Abstract

A dry analytical reagent for detecting gamma-glutamyl transpeptidase comprises an upper support layer, a diffusion layer, a reagent layer, a support and a lower support layer from top to bottom in sequence, wherein the diffusion layer, the reagent layer and the support are arranged at the middle position between the upper support layer and the lower support layer from top to bottom in sequence, the pores at the two ends of the upper support layer and the lower support layer are bonded through the middle support layer, the middle positions of the upper support layer and the lower support layer are respectively provided with a sample adding hole and a test hole, and the porosity of the diffusion layer is 40-75%; the diffusion layer consists of high-crosslinked polymethyl methacrylate microspheres, an adhesive and a surfactant, and the particle size of the high-crosslinked polymethyl methacrylate microspheres is 5-50 mu m; the reagent layer comprises a surfactant, diglycine, L-gamma-glutamyl-3-carboxyl-p-nitroaniline, a buffer solution, a stabilizer and a water-soluble high polymer. The diffusion layer has the characteristic of isotropy, has good detection specificity, high accuracy and wide measurement range, meets the requirement of instant diagnosis and has great clinical application value.

Description

A dry analytical reagent for the detection of γ-glutamyl transpeptidase

technical field

The invention relates to a dry analysis reagent for detecting gamma-glutamyl transpeptidase, which belongs to the field of clinical diagnosis.

Background technique

γ-glutamyltransferase (GGT) is a peptidase that catalyzes γ-glutamyl transfer reactions. Serum GGT is a sensitive indicator of hepatobiliary diseases, and elevated serum GGT can be seen in hepatobiliary diseases caused by various causes. GGT clinical detection is divided into wet chemical method and dry chemical method, among which wet chemical method is a commonly used analysis method, wet chemical method reagent preparation cycle is long, requires professional operation, and the test results are easily affected by the level of operators, while dry chemical analysis The method has the advantages of fast detection speed, easy storage of detection reagents, no need to configure any liquid reagents, low detection threshold requirements, and strong adaptability to occasions. Short requirements have promoted the gradual development of emergency biochemical testing technology from traditional wet chemistry to dry biochemistry. The dry chemical analysis method has a fast test speed and a high level of test automation. It can perform individualized project combination tests on scattered samples anytime, anywhere, and is especially suitable for scenarios such as emergency departments, grassroots clinics, blood stations, physical examination institutions, and testing laboratories.

At present, the commonly used dry analysis reagents continue the coating technology of photosensitive film, and the diffusion layer, light blocking layer, reagent layer and other functional layers are coated on the support in sequence, and the analyte is analyzed by measuring the change of reflected optical density. concentration. The test results of this kind of multilayer film dry film are accurate, but the production process is complicated. The production of the diffusion layer requires the use of various organic solvents, which generates a large amount of organic waste gas, which is harmful to the environment and increases the danger of the production process. Another type of dry chemical analysis reagent consists of a plastic support layer, one end of which is a reaction zone including a sample layer and a reagent layer, and the reagent layer uses fabric as a reagent carrier, and sample addition and testing are performed on the upper part.

Patent CN201910219761.9 discloses an automatic hemofiltration dry-type photochemical microchannel liver function test card for three items, in which the hemofiltration membrane is a commercially available product and tested by transmission photometry, which cannot completely avoid substances in the sample to be tested interference. Patent CN201910236059.3 discloses reagents and diagnostic analysis methods for measuring γ-glutamyl transferase. The diagnostic analysis method steps are relatively cumbersome and still require personnel with certain professional knowledge to operate, and the precision of the detection results of the microfluidic chip is not high enough. ensure.

Contents of the invention

In order to overcome the disadvantages of the prior art, the present invention provides a dry analytical reagent for the detection of γ-glutamyl transpeptidase. The diffusion layer is made of highly cross-linked polymethyl methacrylate microspheres that do not swell when exposed to water and are impermeable. It is combined with a very small amount of specific adhesive to form a high-porosity diffusion layer, which can achieve efficient and rapid penetration and diffusion of the liquid to be analyzed containing γ-glutamyl transpeptidase. It can be evenly distributed, diffused and Rapid delivery of test samples containing analytes.

The technical solution adopted by the present invention to solve its technical problems is:

A dry analysis reagent for the detection of γ-glutamyl transpeptidase, the dry analysis reagent includes an upper support layer, a diffusion layer, a reagent layer, a support and a lower support layer from top to bottom, the diffusion layer , the reagent layer and the support body are sequentially arranged in the middle position between the upper support layer and the lower support layer from top to bottom, and the pores at both ends of the upper support layer and the lower support layer are bonded through the middle support layer, so The upper support layer and the middle position of the lower support layer are respectively provided with a sampling hole and a test hole, and the diffusion layer is an isotropic porous diffusion membrane with a porosity of 40-75%; Composed of linked polymethyl methacrylate microspheres, adhesives and surfactants, the particle size of the highly cross-linked polymethyl methacrylate microspheres is 5-50 μm, and the adhesive concentrates on the highly cross-linked The area adjacent to the adjacent microspheres on the surface of polymethyl methacrylate microspheres, and highly cross-linked polymethyl methacrylate microspheres are bonded into a coherent three-dimensional lattice; the reagent layer includes surfactants, Diglycyl peptide, L-γ-glutamyl-3-carboxy-p-nitroaniline, buffer, stabilizer and water-soluble polymer.

The above-mentioned dry analytical reagent for the detection of γ-glutamyl transpeptidase, the highly cross-linked polymethyl methacrylate microspheres are prepared by suspension polymerization, and the cross-linking agent is selected from methacrylate, dimethyl One of ethylene glycol acrylate, urethane, divinylbenzene and N,N-methylenebisacrylamide or any combination of two or more, the mass of the crosslinking agent accounted for 5wt% to 30wt% of the mass.

The dry analytical reagent for the detection of the above gamma-glutamyl transpeptidase, the binder includes poly(butyl acrylate-styrene), poly(n-butyl acrylate-acrylic acid), polyethyl acrylate, poly(N -isopropylacrylamide), poly(ethyl acrylate-styrene), poly(butyl acrylate-methacrylic acid), poly-n-butyl methacrylate, poly(methyl methacrylate-butyl acrylate) or One of poly(vinyltoluene-tert-butylstyrene-methacrylic acid).

The above-mentioned dry analysis reagent for γ-glutamyl transpeptidase detection, the addition amount of the highly cross-linked polymethylmethacrylate microspheres per unit square meter is 20-500g, and the addition amount of the adhesive per unit square meter accounts for 1.5-4.5% of the added amount per unit square meter of the high cross-linked polymethyl methacrylate microspheres.

The above-mentioned dry analytical reagent for the detection of γ-glutamyl transpeptidase, the surfactant is a nonionic surfactant, including p-octylphenoxypolyoxyethylene ether, p-nonylphenoxypolyoxyethylene ether , polyoxyethylene sorbitan monolaurate or octyl glucoside.

In the above-mentioned dry analytical reagent for detecting γ-glutamyl transpeptidase, the surfactant is preferably a nonionic surfactant with a chain structure consisting of 8 to 15 oxyethylene or oxypropylene groups connected to each other.

The dry analytical reagent for the detection of the above gamma-glutamyl transpeptidase, the buffer is a pH buffer with a pH value in the range of 7.0 to 9.0, and the buffer contains tris (hydroxymethyl) aminomethane (tris ), phosphate, borate, lysine; N, N-bis(2-hydroxyethyl)glycine, N-2-hydroxyethylpiperazine-N'-2-hydroxypropane-3-sulfonic acid Sodium or potassium salt, sodium or potassium salt of N-2-hydroxyethylpiperazine-N'-3-sulfonic acid, N-[tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid Sodium or potassium salt and sodium or potassium salt of N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid, sodium salt of 1,4-piperazine-diethanesulfonic acid (PIPES) or Potassium salt and one of the acids, bases or salts combined with any of the above compounds.

The dry analytical reagent for the detection of the above gamma-glutamyl transpeptidase, the stabilizer is one or more of polysaccharides or disaccharides; the water-soluble polymer is gelatin, gelatin derivatives, agarose , dextran, polyvinyl alcohol, polyacrylamide, a mixture of one or more of hydrophilic cellulose derivatives.

The dry analytical reagent for the detection of γ-glutamyl transpeptidase above, the upper support, the lower support and the middle support are all transparent plastic substrates with a light transmittance of more than 80% under 200nm-900nm wavelength light , including one of polyethylene terephthalate, polycarbonate, polypropylene and polyethylene, with a thickness of 50-300 μm.

The beneficial effects of the present invention are:

No organic solvent is used in the preparation process of the dry analysis reagent for γ-glutamyl transpeptidase detection of the present invention, and the production and preparation process is environmentally friendly; the diffusion layer is made of highly cross-linked polymethylmethacrylate that does not swell when exposed to water and is impermeable Ester microspheres, which are combined with a very small amount of specific binder to form a high-porosity diffusion layer, can achieve efficient and rapid penetration and diffusion of the liquid to be analyzed containing γ-glutamyl transpeptidase, which can be uniform inside Distribution, diffusion and rapid transfer of the sample containing the analyte; L-γ-glutamyl-3-carboxy-p-nitroaniline with good water solubility is used as the chromogenic substrate, and the test results are highly accurate and the measurement range is wide , to meet the needs of instant diagnosis and on-site diagnosis, and has great clinical application value.

Description of drawings

Fig. 1 is the structural representation of multilayer film dry chemical reagent sheet of the present invention;

Fig. 2 is the method of the present invention of embodiment 1 and utilizes the analytical diagram of the correlation of multiple detection samples of gamma-glutamyl carboxyl nitroaniline control method;

Fig. 3 is the method of the present invention of embodiment 2 and utilizes the analytical diagram of the multiple detection sample correlation of gamma-glutamyl carboxyl nitroaniline control method;

Fig. 4 is an analysis diagram of the correlation between the method of the present invention in Example 3 and the control method using γ-glutamylcarboxynitroaniline for multiple detection samples.

In the figure: 1, upper support layer; 1-1, sample injection hole; 2, diffusion layer; 3, reagent layer; 4, support body; 5, lower support layer; 5-1, test hole; 6, middle support layer .

detailed description

In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments It is a part of embodiments of the present invention, but not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

The dry analytical reagent of the present invention can measure γ-glutamyl transpeptidase in body fluid samples. The body fluid may be blood or urine, etc.; blood or urine may be used directly as a body fluid sample, or a sample that has undergone appropriate pretreatment may be used.

The dry analysis reagent of the present invention comprises an upper support layer 1, a diffusion layer 2, a reagent layer 3, a support 4 and a lower support layer 5 from top to bottom, and the diffusion layer, reagent layer and support are arranged in sequence from top to bottom In the middle position between the upper support layer and the lower support layer, the gaps at both ends of the upper support layer and the lower support layer are bonded by the middle support layer 6, and the upper support layer 1 and the lower support layer 5 are in the middle The position is respectively provided with a sampling hole 1-1 and a testing hole 5-1, and the liquid to be tested is dripped onto the diffusion layer from the sampling hole, and the liquid to be tested diffuses rapidly and uniformly in four directions through the diffusion layer and at the same time diffuses longitudinally to the reagent layer. Biochemical reactions occur in the reagent layer. The support is a structural layer that acts as a support and a light-transmitting carrier. The upper support layer, lower support layer and middle support layer are used to fix and protect the support distributed with the reagent layer and diffusion layer. Functional inner packaging structure.

The diffusion layer of the present invention selects highly cross-linked polymethyl methacrylate microspheres that do not swell when exposed to water and are impermeable, and are combined with a very small amount of specific adhesive to form a diffusion layer with a high-porosity structure. The surface of the linked polymethyl methacrylate microspheres and the adjacent area of the adjacent microspheres bond the highly cross-linked polymethyl methacrylate microspheres into a coherent three-dimensional lattice, and this three-dimensional lattice structure exhibits a high void Volume, control the particle size distribution of highly cross-linked polymethyl methacrylate microspheres and the amount of binder added, so as to form various uniform porous diffusion layers with different porosity and pore size distribution for rapid absorption and uniform distribution , metering and rapid delivery of multiple analytes to analyze substances of different molecular weight distributions and compositions in biological fluids.

The highly cross-linked polymethyl methacrylate microspheres of the present invention are prepared by suspension polymerization technology. In order to meet the non-swelling and impermeability requirements of the polymer microspheres, it is necessary to add an appropriate cross-linking agent during the polymerization of the microspheres to obtain high-strength cross-linking. linking structure, the crosslinking agent in the present invention is preferably divinylbenzene, and the crosslinking agent addition is preferably 5wt%～2wt% of the monomer quality of methyl methacrylate MMA; The particle size of methyl methacrylate microspheres is 5-50 μm, the amount added per unit square meter is 20-500 g, preferably 50-300 g per unit square meter, and the amount added per unit square meter of adhesive accounts for The amount of methyl ester microspheres added is 1.5-4.5%, and the porosity of the control diffusion layer is 40-75%, which can form a large number of diffusion channels from hundreds of nanometers to several microns, and the relative molecular mass of γ-glutamyl transpeptidase At about 90kDa, the molecular size is tens of nanometers, the diffusion channel of the diffusion layer can allow the rapid distribution, metering and transmission of water, small molecular substances and γ-glutamyl transpeptidase, and prevent the passage of red blood cells and other macromolecular substances to form Effective filtering action.

The chromogenic substrate in the reagent layer of the present invention is selected from L-γ-glutamyl-3-carboxy-p-nitroaniline with good water solubility. The commonly used γ-glutamyl transpeptidase detection kits basically use L-γ-glutamyl-p-nitroaniline as the chromogenic substrate, but L-γ-glutamyl-p-nitroaniline exists in water-soluble Due to the disadvantages of poor performance and low color density, it is difficult to reach a saturated substrate concentration, and cannot show the maximum activity of L-γ-glutamyl transferase. After screening, the present invention replaces the substrate L-γ-glutamyl-3-carboxy-p-nitroaniline with good water solubility as the color development substrate. The amount of L-γ-glutamyl-3-carboxy-p-nitroaniline added per square meter is 0.01-1 g, preferably 0.02-0.5 g per square meter.

The addition amount of the surfactant in the reagent layer is 1-25g per unit square meter, preferably 2-15g per unit square meter; 0.1-10g per unit square meter of glycylidene, preferably 0.2-5g per unit square meter; stabilizer per unit square meter The addition amount is 0.2-10g; the water-soluble polymer is 1-50g per square meter, preferably 2-25g per square meter.

The preparation method of the dry analysis reagent of the present invention is specifically:

(1) Reagent layer The reagent solution is coated on the support, and the coating method can use known methods, preferably wire bar coating, knife coating, extrusion coating, and the wet film thickness of the reagent layer is preferably 50-500 μm, Dry in the range of 10°C-60°C, and the dry film thickness of the reagent layer is preferably 5-50 μm.

(2) The diffusion layer slurry is coated on the (1) reagent layer, and the coating method can use known methods, preferably wire bar coating, blade coating, extrusion coating, and the wet film thickness of the diffusion layer is preferably 60 -600μm, dry in the range of 10℃-60℃, the dry film thickness of the diffusion layer is preferably 20-200μm.

(3) Cut the materials prepared in step (2) into small pieces of 1.2 cm ² , use custom-made white PET plastic sheets as the upper support layer, lower support layer and middle support layer, fix and assemble them into dry analysis reagents.

γ-glutamyl transpeptidase detection of the present invention uses the dry analysis reagent detection method as follows:

Add about 10 μL of the liquid to be tested dropwise on the homogeneous porous diffusion layer, and incubate at about 37°C for 5 minutes. concentration distribution. GGT catalyzes the transfer of the glutamate moiety of L-γ-glutamyl-p-nitroaniline to glycylglycine, and simultaneously produces p-nitroaniline. The reflection density detection of the color change reaction produced by the dry analytical reagent is carried out from the support side by the rate method, and the concentration of GGT in the liquid to be tested is calculated using a pre-made calibration curve. The equation for the reaction of the target substance GGT with the corresponding substance in the dry analysis reagent is as follows:

The preparation process of the dry analysis reagent for gamma-glutamyl transpeptidase detection does not require organic solvents, is environmentally friendly, has good stability, is easy to store and keep, and has high detection accuracy and wide linear range.

The present invention will be further described below in conjunction with embodiment.

Example 1

On a colorless and transparent PET film support 4 with a thickness of 175 μm, the reagent layer coating liquid composed of the following Table 1 was coated and dried at a constant temperature of 40° C., so that the thickness after drying reached 15 μm. Next, the diffusion layer coating solution with the composition shown in Table 2 below was coated on the above coating layer and dried at a constant temperature of 35° C., so that the thickness after drying was 100 μm. Cut into small pieces of ^1.2 cm after drying, and then use white PET plastic sheets as the upper support layer 1, the lower support layer 5 and the middle support layer 6, which can be used for measurement after assembly.

Table 1 Reagent Layer Recipe

Reagent Coating amount/m² gelatin 10g Diglycine 1.8g L-γ-glutamyl-3-carboxy-p-nitroaniline 200mg PIPES buffer pH7.7 Triton X-100 1.5g sucrose 1.2g

Table 2 Diffusion layer formulation

Material Coating amount/m² Highly cross-linked polymethylmethacrylate microspheres (10μm) 150g Poly(n-butyl acrylate-acrylic acid) emulsion 3g Surfactant 1.5g

For 25 clinical serum samples, the method of the present invention was used to analyze the correlation of multiple detection samples using the modified γ-glutamylcarboxynitroaniline control method. The results are shown in Fig. 2, and the present invention can obtain a good correlation compared with the benchmark reference method.

Example 2

On a colorless and transparent PET film support 4 with a thickness of 175 μm, the reagent layer coating solution composed in Table 3 below was coated and dried at a constant temperature of 37° C., so that the thickness after drying reached 10 μm. Next, the diffusion layer coating liquid with the composition shown in Table 4 below was coated on the above coating layer and dried at a constant temperature of 40° C., so that the thickness after drying was 150 μm. Cut into small pieces of ^1.2 cm after drying, and then use white PET plastic sheets as the upper support layer 1, the lower support layer 5 and the middle support layer 6, which can be used for measurement after assembly.

Table 3 Reagent Layer Recipe

Reagent Coating amount/m² gelatin 10g Diglycine 3.5g L-γ-glutamyl-3-carboxy-p-nitroaniline 150mg PIPES buffer pH8.0 Triton X-100 1.5g sucrose 2.0g

Table 4 Diffusion Layer Recipe

Material Coating amount/m² Highly cross-linked polymethyl methacrylate microspheres (30μm) 100g Poly(butyl acrylate-styrene) emulsion 1.5g Surfactant 1g

For 25 clinical serum samples, the method of the present invention was used to analyze the correlation of multiple detection samples using the modified γ-glutamylcarboxynitroaniline control method. The results are shown in Fig. 3. Compared with the reference method, the present invention can obtain a good correlation.

Example 3

On a colorless and transparent PET film support 4 with a thickness of 175 μm, the reagent layer coating liquid composed in Table 5 below was coated and dried at a constant temperature of 40° C., so that the thickness after drying reached 20 μm. Next, the diffusion layer coating solution with the composition shown in Table 6 below was coated on the above coating layer and dried at a constant temperature of 45° C., so that the thickness after drying was 100 μm. Cut into small pieces of ^1.2 cm after drying, and then use white PET plastic sheets as the upper support layer 1, the lower support layer 5 and the middle support layer 6, which can be used for measurement after assembly.

Table 5 Reagent Layer Recipe

Reagent Coating amount/m² gelatin 10g Diglycine 1.0g L-γ-glutamyl-3-carboxy-p-nitroaniline 100mg Tris buffer pH7.8 Triton X-100 1.5g sucrose 0.6g

Table 6 Diffusion layer formulation

Material Coating amount/m² Highly cross-linked polymethyl methacrylate microspheres (45μm) 150g Poly(butyl acrylate-methacrylic acid) emulsion 4g Surfactant 2g

For 25 clinical serum samples, the method of the present invention was used to analyze the correlation of multiple detection samples using the modified γ-glutamylcarboxynitroaniline control method. The results are shown in Fig. 4. Compared with the benchmark reference method, the present invention can obtain good correlation.

The above-mentioned embodiments are only to illustrate the technical concept and characteristics of the present invention, and its purpose is to enable those of ordinary skill in the art to understand the content of the present invention and based on the facts, and cannot limit the protection scope of the present invention. All equivalent replacements or improvements made according to the essence of the content of the present invention shall be included within the scope of the claims of the present invention.

Claims (9)

1. A dry analysis reagent for gamma-glutamyl transpeptidase detection, the dry analysis reagent comprises an upper support layer (1), a diffusion layer (2), a reagent layer (3), A support (4) and a lower support layer (5), the diffusion layer, the reagent layer and the support are sequentially arranged in the middle between the upper support layer and the lower support layer from top to bottom, and the upper support layer and the two ends of the lower support layer are bonded through the middle support layer (6), and the middle positions of the upper support layer (1) and the lower support layer (5) are respectively provided with a sample hole (1-1) and a test hole (5-1), characterized in that: the diffusion layer is an isotropic porous diffusion membrane with a porosity of 40-75%; the diffusion layer is made of highly cross-linked polymethyl methacrylate microspheres, Composed of an adhesive and a surfactant, the particle diameter of the highly cross-linked polymethyl methacrylate microspheres is 5-50 μm, and the adhesive is concentrated on the surface of the highly cross-linked polymethyl methacrylate microspheres and Adjacent areas of adjacent microspheres, and highly cross-linked polymethyl methacrylate microspheres bonded into a coherent three-dimensional lattice; - Glutamyl-3-carboxy-p-nitroaniline, buffer, stabilizer and water-soluble polymer. 2. The dry analytical reagent for γ-glutamyl transpeptidase detection according to claim 1, characterized in that: the highly cross-linked polymethyl methacrylate microspheres are prepared by suspension polymerization, cross-linked The coupling agent is selected from one of methacrylate, ethylene glycol dimethacrylate, urethane, divinylbenzene and N,N-methylenebisacrylamide, or any combination of two or more. The mass of the coupling agent accounts for 5wt%-30wt% of the mass of the methyl methacrylate MMA monomer. 3. The dry analytical reagent for gamma-glutamyl transpeptidase detection according to claim 2, characterized in that: the adhesive comprises poly(butyl acrylate-styrene), poly(n-butyl acrylate -acrylic acid), polyethyl acrylate, poly(N-isopropylacrylamide), poly(ethyl acrylate-styrene), poly(butyl acrylate-methacrylic acid), poly-n-butyl methacrylate, poly (methyl methacrylate-butyl acrylate) or poly(vinyltoluene-tert-butylstyrene-methacrylic acid). 4. The dry analytical reagent for γ-glutamyl transpeptidase detection according to claim 3, characterized in that: the amount of highly cross-linked polymethylmethacrylate microspheres per unit square meter is 20-500g, The added amount per unit square meter of the adhesive accounts for 1.5-4.5% of the added amount per unit square meter of the highly cross-linked polymethyl methacrylate microspheres. 5. The dry analytical reagent for gamma-glutamyl transpeptidase detection according to claim 4, characterized in that: the surfactant is a nonionic surfactant, including p-octylphenoxypolyoxyethylene Ether, p-nonylphenoxy polyoxyethylene ether, polyoxyethylene sorbitan monolaurate or octyl glucoside. 6. The dry analytical reagent for gamma-glutamyl transpeptidase detection according to claim 5, characterized in that: the surfactant is preferably composed of 8 to 15 oxyethylene or oxypropylene groups connected to each other Composed of nonionic surfactants with a chain structure. 7. The dry analytical reagent for detecting γ-glutamyl transpeptidase according to claim 6, characterized in that: the buffer is a pH buffer with a pH value in the range of 7.0 to 9.0, and the buffer is Contains tris (hydroxymethyl) aminomethane (tris), phosphate, borate, lysine; N, N-bis (2-hydroxyethyl) glycine, N-2-hydroxyethylpiperazine-N Sodium or potassium salt of '-2-hydroxypropane-3-sulfonic acid, sodium or potassium salt of N-2-hydroxyethylpiperazine-N'-3-sulfonic acid, N-[tri(hydroxymethyl )Methyl]-3-aminopropanesulfonic acid sodium or potassium salt and N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid sodium or potassium salt, 1,4-piperazine - Sodium or potassium salt of diethanesulfonic acid (PIPES) and one of the acids, bases or salts in combination with any of the above compounds. 8. The dry analytical reagent for γ-glutamyl transpeptidase detection according to claim 7, characterized in that: the stabilizer is one or more of polysaccharides or disaccharides; the water-soluble high The molecular polymer is one or more mixtures of gelatin, gelatin derivatives, agarose, dextran, polyvinyl alcohol, polyacrylamide and hydrophilic cellulose derivatives. 9. The dry analytical reagent for γ-glutamyl transpeptidase detection according to claim 8, characterized in that: the upper support (1), the lower support (5) and the middle support (6) Both are transparent plastic substrates with a light transmittance of more than 80% under 200nm-900nm wavelength light, including one of polyethylene terephthalate, polycarbonate, polypropylene and polyethylene, and its thickness is 50-300μm.

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