CN115372151A - Spinal column loading system and method - Google Patents

Abstract

The invention relates to the technical field of medical equipment, in particular to a spinal column loading system and a spinal column loading method. In the device, a culture box contains a force loading mechanism of a spine, the force loading mechanism is provided with a culture dish, the culture dish is used for containing the spine, a storage box contains a storage container used for containing culture solution, a liquid inlet pump is respectively communicated with the storage container and the culture dish through a pipeline, one end of a liquid outlet pump is communicated with the bottom of the culture dish through a pipeline, the other end of the liquid outlet pump is communicated with an external waste liquid box or the storage container through a pipeline, in the force loading mechanism, a frame body comprises two guide pillars, a first flat plate, a second flat plate, a third flat plate and a fourth flat plate, and the upper end and the lower end of the spine are respectively fixed on a first mounting seat and a second mounting seat; the axial loading mechanism is used for applying acting force along the axial direction of the spine to the first mounting seat so as to axially load the spine; the circumferential loading mechanism is used for applying acting force along the circumferential direction of the spine to the first installation seat so as to circumferentially load the spine.

Description

Spinal column loading system and method

Technical Field

The invention relates to the technical field of medical equipment, in particular to a spinal column loading system and a spinal column loading method.

Background

Mechanical loading is critical to maintaining the structure and function of the bone. The bone tissue has adaptability to the mechanical environment, when the mechanical load on the bone tissue is reduced, bone loss occurs, the structural strength of the bone tissue is reduced, and thus the mechanical property of the whole bone is reduced.

In the related art, the spine is generally used as a subject to be studied for the study of mechanical properties. Most of the related art studies are axial loading of the spine, which is not conducive to study of the overall mechanics of the spine. Further, the related art is to accommodate the spine in a culture dish filled with a culture solution and apply an external force to the spine cultured in vitro to study the influence of the external force on the intervertebral disc tissue. Above-mentioned scheme needs the staff to hold the syringe and takes out the culture solution from storage container, then pours into the culture solution inlet port of culture dish into the culture solution in with the syringe, utilizes the syringe to take out the culture solution from the culture dish after the experiment is accomplished. Obviously, this solution may cause contamination of the culture solution during the transfer of the culture solution.

In view of the foregoing, there is a need for a spinal loading system and method that solves the above-mentioned problems.

Disclosure of Invention

The invention provides a spinal column loading system and a spinal column loading method, which can realize the research on the overall mechanical characteristics of a spinal column and avoid the pollution of a culture solution in the process of transferring the culture solution.

In a first aspect, embodiments of the present invention provide a spinal loading system, including:

the culture box is internally provided with a force loading mechanism with spines, the force loading mechanism is provided with a culture dish with an opening, the culture dish is used for containing the spines, and the culture box is used for providing a constant-temperature and constant-humidity environment;

a storage box, in which a storage container for containing a culture solution is accommodated, for providing an environment of a storage temperature of the culture solution;

the two ends of the liquid inlet pump are respectively communicated with the storage container and the culture dish through pipelines and are used for conveying the culture liquid in the storage container to the culture dish;

one end of the liquid outlet pump is communicated with the bottom of the culture dish through a pipeline, and the other end of the liquid outlet pump is communicated with an external waste liquid box or the storage container through a pipeline and is used for conveying the culture liquid in the culture dish into the waste liquid box or the storage container;

the force loading mechanism includes:

the frame body comprises two guide posts extending along the axial direction of a spine, and a first flat plate, a second flat plate, a third flat plate and a fourth flat plate which are sequentially arranged from top to bottom along the axial direction of the spine, wherein the first flat plate and the fourth flat plate are both fixed with the guide posts, the second flat plate and the third flat plate are fixedly connected, and the second flat plate and the third flat plate can both move upwards or downwards along the guide posts;

the axial loading mechanism is fixed on the first flat plate;

a circumferential loading mechanism disposed between the second plate and the third plate;

the upper end and the lower end of the spine are respectively fixed on a first mounting seat and a second mounting seat, the first mounting seat is rotatably connected with the third flat plate, the first mounting seat can rotate along the circumferential direction of the spine, and the second mounting seat is fixed with the fourth flat plate;

the axial loading mechanism is used for applying acting force along the axial direction of the spine to the first mounting seat so as to axially load the spine;

the circumferential loading mechanism is used for applying acting force along the circumferential direction of the spine to the first mounting seat so as to circumferentially load the spine.

In a second aspect, an embodiment of the present invention provides a loading method for a spinal column, which is based on the loading system for a spinal column according to any one of the above embodiments, and the method includes:

before the force loading mechanism carries out mechanical experiment on the spine, the culture solution in the storage container is conveyed into the culture dish through the liquid inlet pump;

after the mechanical experiment of the force loading mechanism on the spine is completed, the culture solution in the culture dish is conveyed to the waste liquid tank through the liquid outlet pump; or, in the process of carrying out the mechanical experiment of the spine, the culture solution in the culture dish is conveyed into the storage container through the liquid outlet pump;

wherein, the force loading mechanism carries out mechanics experiment to the backbone, includes:

when the force loading mechanism loads the spine axially, the axial loading mechanism is controlled to apply acting force along the axial direction of the spine to the first mounting seat so as to load the spine axially;

when the force loading mechanism carries out circumferential loading on the spine, acting force along the circumferential direction of the spine is applied to the first mounting seat by controlling the circumferential loading mechanism so as to carry out circumferential loading on the spine.

According to the scheme, the axial loading mechanism is arranged on the first flat plate, so that the acting force along the axial direction of the spine can be applied to the first mounting seat, and the spine is axially loaded; by providing a circumferential loading mechanism between the second plate and the third plate, it is possible to apply a force to the first mounting block in the circumferential direction of the spine to circumferentially load the spine. Therefore, the above solution allows to study the overall mechanical characteristics of the spinal column. And, the loading system of backbone is through setting up the incubator, the storage box, feed liquor pump and play liquid pump, just so can be before power loading mechanism exerts the force load to the backbone, utilize the feed liquor pump will be arranged in the culture solution of the storage container of storage box and carry the culture dish that is arranged in the culture dish, and after the mechanical experiment to the backbone is accomplished, utilize the culture solution of going out the liquid pump in with the culture dish to carry outside in the waste liquid case, or at the in-process that carries out the mechanical experiment of backbone, carry the storage container through the culture solution of going out the liquid pump in with the culture dish, so can avoid causing the pollution of culture solution at the in-process that shifts the culture solution.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained based on these drawings without creative efforts.

FIG. 1 is a schematic structural view of a spinal loading system (i.e., including a spinal semi-circulatory apparatus and a force loading mechanism) according to one embodiment of the present invention;

FIG. 2 is a schematic structural view of a spinal loading system (i.e., including a spinal total circulation device and a force loading mechanism) according to another embodiment of the present invention;

FIG. 3 is a schematic diagram of a structure of a culture dish according to an embodiment of the invention;

FIG. 4 is a schematic view of another embodiment of the present invention;

FIG. 5 is a schematic structural diagram of a force loading mechanism provided in accordance with one embodiment of the present invention;

FIG. 6 is an enlarged schematic view at A in FIG. 5;

FIG. 7 is a front view of the force loading mechanism of FIG. 5;

FIG. 8 is an enlarged schematic view at B of FIG. 7;

FIG. 9 is a schematic structural diagram at B of a force loading mechanism provided in accordance with another embodiment of the present invention;

FIG. 10 is a cross-sectional schematic view of the force loading mechanism of FIG. 5;

FIG. 11 is an enlarged schematic view at C of FIG. 10;

fig. 12 is an enlarged schematic view at D in fig. 10.

Reference numerals:

11-an incubator; 12-a storage box; 13-a liquid inlet pump; 14-a liquid outlet pump; 15-an anti-overflow pump; 16-a pipeline; 17-a flow sensor; 18-a pressure sensor; 19-waste liquid tank; 111-a liquid inlet interface; 112-liquid outlet interface; 113-an anti-spill interface; 121-a storage container;

21-a frame body; 22-axial loading mechanism; 23-a circumferential loading mechanism; 24-culture dish; 25-a first mount; 26-a second mount; 27-a mounting groove; 211-guide pillars; 212-a first plate; 213-a second plate; 214-a third plate; 215-fourth plate; 216-fifth plate; 221-a first output shaft; 222-a first connector; 223-a first force sensor; 231-a second output shaft; 232-a rotating assembly; 233-connecting components; 234-a second force sensor; 232 a-axis of rotation; 232 b-a fixed seat; 232 c-bearing; 233 a-a second connector; 233 b-a third connector; 233 c-a fourth connection; 241-liquid inlet; 242-a liquid outlet; 243-overflow prevention port; 244-a splash cover; 245-bone cement injection port; 246-a platen; 247-O-rings; 248-a silica gel pad; 249-fixed station.

Detailed Description

To make the objects, technical solutions and advantages of the embodiments of the present invention clearer and more complete, the technical solutions in the embodiments of the present invention will be described below with reference to the drawings in the embodiments of the present invention, it is obvious that the described embodiments are some, but not all embodiments of the present invention, and based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

Referring to fig. 1 and 2, one embodiment of the present invention provides a spinal loading system comprising:

the incubator 11 is internally provided with a force loading mechanism with spines, the force loading mechanism is provided with a culture dish 24 with an opening, the culture dish 24 is used for accommodating the spines, and the incubator 11 is used for providing a constant-temperature and constant-humidity environment;

a storage box 12, in which a storage container 121 for containing a culture solution is housed, the storage box 12 being used to provide an environment of a storage temperature of the culture solution;

a liquid inlet pump 13, both ends of which are respectively communicated with the storage container 121 and the culture dish 24 through a pipeline 16, for conveying the culture liquid in the storage container 121 to the culture dish 24;

a liquid outlet pump 14, one end of which is communicated with the bottom of the culture dish 24 through a pipeline 16, and the other end of which is communicated with an external waste liquid box 19 or a storage container 121 through a pipeline 16, for conveying the culture liquid in the culture dish 24 to the waste liquid box 19 or the storage container 121;

a force loading mechanism comprising:

the frame body 21 comprises two guide posts 211 extending along the axial direction of the spine, and a first flat plate 212, a second flat plate 213, a third flat plate 214 and a fourth flat plate 215 which are sequentially arranged from top to bottom along the axial direction of the spine, wherein the first flat plate 212 and the fourth flat plate 215 are both fixed with the guide posts 211, the second flat plate 213 and the third flat plate 214 are fixedly connected, and the second flat plate 213 and the third flat plate 214 can both move upwards or downwards along the guide posts 211;

an axial loading mechanism 22 fixed to the first plate 212;

a circumferential loading mechanism 23 provided between the second plate 213 and the third plate 214;

the upper end and the lower end of the spine are respectively fixed on the first mounting seat 25 and the second mounting seat 26, the first mounting seat 25 is rotatably connected with the third flat plate 214, the first mounting seat 25 can rotate along the circumferential direction of the spine, and the second mounting seat 26 is fixed with the fourth flat plate 215;

the axial loading mechanism 22 is used for applying a force along the axial direction of the spine to the first mounting seat 25 so as to axially load the spine;

the circumferential loading mechanism 23 is used to apply a force to the first mount 25 in the circumferential direction of the spine to circumferentially load the spine.

In this embodiment, by providing the axial loading mechanism 22 on the first plate 212, it is possible to apply a force to the first mounting seat 25 in the axial direction of the spine to axially load the spine; by providing a circumferential loading mechanism 23 between the second plate 213 and the third plate 214, it is possible to apply a force to the first mounting block 25 in the circumferential direction of the spine to circumferentially load the spine. Therefore, the above solution allows to study the overall mechanical characteristics of the spinal column. Moreover, the loading system of the spine is provided with the culture box 11, the storage box 12, the liquid inlet pump 13 and the liquid outlet pump 14, so that the culture solution in the storage container 121 in the storage box 12 can be conveyed to the culture dish 24 in the culture box 11 by the liquid inlet pump 13 before the force loading mechanism applies force load to the spine, and after the mechanical experiment on the spine is completed, the culture solution in the culture dish 24 is conveyed to the external waste liquid box 19 by the liquid outlet pump 14, or in the process of performing the mechanical experiment on the spine, the culture solution in the culture dish 24 is conveyed to the storage container 121 by the liquid outlet pump 14, so that the pollution of the culture solution caused in the process of transferring the culture solution can be avoided.

The components of the spinal loading system are described separately below.

Referring to FIG. 1, one embodiment of the present invention provides a spinal loading system that includes a semi-circulation device for spinal culture fluid and a force loading mechanism (i.e., a bi-directional loading device, wherein bi-directional is axial and circumferential, respectively).

Referring to fig. 2, another embodiment of the present invention provides a spinal loading system, which includes a device for complete circulation of spinal culture fluid and a force loading mechanism.

It will be appreciated that the difference between the loading system of figure 2 and the loading system of figure 1 is the means for circulating the culture solution through the spinal column.

Referring to fig. 1, in one embodiment of the present invention, the spinal column loading system comprises a circulation device (i.e. a semi-circulation device) including an incubator 11, a preservation chamber 12, an inlet pump 13 and an outlet pump 14, wherein:

the interior of the incubator 11 accommodates a force loading mechanism of a spine, the force loading mechanism is used for applying force load to the spine, the force loading mechanism is provided with a culture dish 24 with an opening, the culture dish 24 is used for accommodating the spine, and the incubator 11 is used for providing a constant temperature and constant humidity environment;

a storage container 121 for containing a culture solution is housed inside the storage box 12, and the storage box 12 is used for providing an environment of a storage temperature of the culture solution;

two ends of the liquid inlet pump 13 are respectively communicated with the storage container 121 and the culture dish 24 through a pipeline 16, and are used for conveying the culture liquid in the storage container 121 to the culture dish 24;

the two ends of the liquid outlet pump 14 are respectively communicated with the bottom of the culture dish 24 and the external waste liquid tank 19 through the pipelines 16, and are used for conveying the culture liquid in the culture dish 24 to the waste liquid tank 19.

In this embodiment, by providing the culture box 11, the storage box 12, the liquid inlet pump 13 and the liquid outlet pump 14, the culture solution in the storage container 121 in the storage box 12 can be conveyed to the culture dish 24 in the culture box 11 by the liquid inlet pump 13 before the force loading mechanism applies a force load to the spine, and after the mechanical experiment on the spine is completed, the culture solution in the culture dish 24 is conveyed to the external waste liquid tank 19 by the liquid outlet pump 14, so that the contamination of the culture solution caused in the process of transferring the culture solution can be avoided.

Referring to fig. 2, in one embodiment of the present invention, the spinal column loading system includes a circulation device (i.e., a full circulation device) including an incubator 11, a preservation chamber 12, an intake pump 13, an outflow pump 14, two flow sensors 17, and a pressure sensor 18, wherein:

the interior of the incubator 11 accommodates a force loading mechanism of a spine, the force loading mechanism is used for applying force load to the spine, the force loading mechanism is provided with a culture dish 24 with an opening, the culture dish 24 is used for accommodating the spine, and the incubator 11 is used for providing a constant temperature and constant humidity environment;

a storage container 121 for containing a culture solution is housed inside the storage box 12, and the storage box 12 is used for providing an environment of a storage temperature of the culture solution;

two ends of the liquid inlet pump 13 are respectively communicated with the storage container 121 and the culture dish 24 through pipelines 16, and are used for conveying the culture liquid in the storage container 121 to the culture dish 24;

two ends of the liquid outlet pump 14 are respectively communicated with the bottom of the culture dish 24 and the storage container 121 through the pipelines 16, and are used for conveying the culture liquid in the culture dish 24 to the storage container 121;

the two flow sensors 17 are respectively arranged on a pipeline 16 connected with the liquid inlet pump 13 and a pipeline 16 connected with the liquid outlet pump 14;

a pressure sensor 18 is arranged on the pipe 16 connected with the liquid inlet pump 13;

the ratio of the flow sensor 17 to the pressure sensor 18 is in a preset range by adjusting the liquid inlet pump 13 and the liquid outlet pump 14; wherein the values of both flow sensors 17 remain the same at all times.

In this embodiment, by providing the incubator 11, the storage chamber 12, the liquid inlet pump 13, the liquid outlet pump 14, two flow sensors 17 and one pressure sensor 18, the culture fluid in the storage container 121 in the storage chamber 12 can be delivered to the culture dish 24 in the incubator 11 by the liquid inlet pump 13 before the force loading mechanism applies a force load to the spinal column, and during the mechanical experiment of the spinal column by the force loading mechanism, the culture fluid in the culture dish 24 is delivered to the storage container 121 by the liquid outlet pump 14, and the liquid inlet pump 13 and the liquid outlet pump 14 are kept working simultaneously, so that the ratio of the flow sensors 17 to the pressure sensor 18 is within a preset range, wherein the values of the two flow sensors 17 are always kept the same. Therefore, the scheme can avoid the pollution of the culture solution in the process of transferring the culture solution.

It can be understood that the more the environment of the loading experiment on the ex-vivo spine is close to the actual situation, the more the obtained mechanical data is close to the real mechanical data of the in-vivo spine, and therefore, the force loading mechanism needs to be arranged in the incubator 11 in an environment capable of being constant in temperature and humidity. Similarly, the culture solution also needs to be in a certain temperature environment (for example, 4 ℃), and therefore the storage container 121 needs to be set in the storage box 12 that can provide an environment at which the culture solution is stored. In some embodiments, the holding tank 12 may be a refrigerator, and is not limited thereto.

To further ensure that the resulting mechanical data is closer to the true mechanical data of the in-vivo spine, changes in blood flow and blood pressure of a true in-vivo body can be simulated by providing two flow sensors 17 and one pressure sensor 18. It should be noted that, since both of the two quantities are variables, for convenience of control, the control variable is simplified to control the ratio of the flow sensor 17 and the pressure sensor 18, that is, the ratio of the two is controlled within a preset range, so as to approximately simulate the real environment. It should be further noted that, this solution only approximates the ratio of the flow rate and the pressure to the real environment, but cannot guarantee that the flow rate and the pressure are respectively approximated to the real environment.

To establish a crossover path between the storage vessel 121 and the culture dish 24, a line 16 may be provided; in order to ensure the conveying power of the culture solution, the liquid inlet pump 13 and the liquid outlet pump 14 may be arranged, that is, before the force loading mechanism applies force load to the spine, the culture solution in the storage container 121 in the storage box 12 is conveyed to the culture dish 24 in the culture box 11 by the liquid inlet pump 13, and during the mechanical experiment of the spine by the force loading mechanism, the culture solution in the culture dish 24 is conveyed to the storage container 121 by the liquid outlet pump 14, and the liquid inlet pump 13 and the liquid outlet pump 14 are kept working simultaneously, so that the ratio of the flow sensor 17 to the pressure sensor 18 is within a preset range. Therefore, the culture solution of the full-circulation device provided by the embodiment of the invention is not only used in a mechanical experiment (for example, the mechanical experiment can be 8 hours), that is, the activity of the culture solution can be kept at a high level through the full-circulation device, and even after one mechanical experiment, the culture solution can be continuously used in a subsequent mechanical experiment, so that the long-time and efficient use of the culture solution is further ensured on the basis of ensuring that the pollution of the culture solution in the transfer process of the culture solution is avoided.

In one embodiment of the present invention, the incubator 11 is filled with carbon dioxide gas, so that the environment of the incubator 11 can be more consistent with the real living environment of the spinal column in vivo.

In one embodiment of the present invention, the opening of the culture dish 24 is provided with a splash cover 244 (see FIG. 4), and the splash cover 244 is in a half-and-half design.

In this embodiment, since the culture solution may generate bubbles in the process of entering the culture dish 24, the bubbles rise to the liquid level of the culture solution and burst, and a part of the culture solution may splash out of the culture dish 24 from the opening of the culture dish 24, which may pollute the external environment of the culture dish 24, the splash cover 244 may be disposed at the opening, and the splash cover 244 may be further installed easily, so that the splash cover 244 is designed in half.

In one embodiment of the present invention, the side wall of the culture dish 24 is provided with a bone cement injection port 245, and the bone cement injection port 245 is used for injecting bone cement into the culture dish 24 at a position for fixing the spine to fix the spine.

In this embodiment, the bone cement is injected through the bone cement injection port 245 provided on the entire body of the culture dish 24, so that the spinal column can be effectively fixed. The embodiment of the invention does not adopt a threaded fastener in the related art, but adopts a bone cement fixing mode, so that the culture solution cannot be polluted by the threaded fastener.

Referring to fig. 10, each of the first and second mounting seats 25 and 26 is provided therein with a mounting groove 27 for receiving a spinal column, the mounting groove 27 is filled with bone cement, and upper and lower ends of the spinal column are fixed to the first and second mounting seats 25 and 26, respectively, by means of embedding the bone cement (i.e., injecting the bone cement through a bone cement injection port 245 provided on the circumference of the culture dish 24). It is understood that the first and second mounting seats 25 and 26 can be made of PP material, which does not contaminate the culture solution. Further, the fasteners used to secure the second mounting base 26 may be titanium alloy, so that the culture solution is not contaminated.

Referring to fig. 12, in an embodiment of the present invention, in order to facilitate the replacement of the second mounting seat 26, it is considered that a pressing plate 246 is disposed at the bottom inside the culture dish 24, and the pressing plate 246 can be fixed to a fixing table 249 by a fastener passing through a through hole (see fig. 4) formed at the bottom of the culture dish 24; to further ensure that the culture solution does not leak out of the through hole formed in the bottom of the culture dish 24, an O-ring 247 may be provided on the bottom of the pressure plate 246.

In one embodiment of the present invention, the half-cycle apparatus further comprises:

an anti-overflow pump 15, both ends of which are respectively communicated with the top of the culture dish 24 and the waste liquid tank 19 through a pipeline 16, for conveying the culture liquid reaching a preset height of the culture dish 24 into the waste liquid tank 19;

the operation of the liquid inlet pump 13 and the overflow preventing pump 15 is controlled to stop in response to the overflow preventing pump 15 delivering the culture liquid into the waste liquid tank 19.

In this embodiment, through setting up anti-overflow pump 15, can guarantee that the culture solution can not spill over from culture dish 24 to can enough guarantee that incubator 11 is not contaminated, can guarantee again that the liquid level of the culture solution in culture dish 24 is in predetermineeing the height, in order to do benefit to the cultivation of carrying out the separation backbone.

In one embodiment of the present invention, the full circulation apparatus further comprises:

an anti-overflow pump 15, both ends of which are respectively communicated with the top of the culture dish 24 and the waste liquid tank 19 through a pipeline 16, for conveying the culture liquid reaching a preset height of the culture dish 24 into the waste liquid tank 19;

in response to spill-proof pump 15 delivering culture solution to waste tank 19, spill-proof pump 15 is controlled to stop operating, and simultaneously effluent pump 14 is controlled to start operating.

In this embodiment, through setting up anti-overflow pump 15, can guarantee that the culture solution can not spill over from culture dish 24 to can enough guarantee that incubator 11 is not contaminated, can guarantee again that the liquid level of the culture solution in culture dish 24 is in predetermineeing the height, in order to do benefit to the cultivation of carrying out the separation backbone. When the anti-overflow pump 15 delivers the culture solution into the waste solution tank 19, the anti-overflow pump 15 needs to be controlled to stop working, and the effluent pump 14 needs to be controlled to start working at the same time, so that the full-circulation process of the culture solution is realized.

In one embodiment of the present invention, the top of the incubator 11 is provided with a liquid inlet port 111, a liquid outlet port 112 and an anti-overflow port 113, the pipe 16 of the liquid inlet pump 13 communicating with the culture dish 24 passes through the liquid inlet port 111, the pipe 16 of the liquid outlet pump 14 communicating with the culture dish 24 passes through the liquid outlet port 112, and the pipe 16 of the anti-overflow pump 15 communicating with the culture dish 24 passes through the anti-overflow port 113.

In this embodiment, the liquid inlet port 111, the liquid outlet port 112 and the anti-overflow port 113 are disposed at the top of the incubator 11, so as to facilitate the assembly and disassembly of the pipeline 16.

Of course, the liquid inlet port 111, the liquid outlet port 112, and the anti-overflow port 113 may not be provided, and are not limited herein.

In another embodiment of the present invention, the semi-circulation device further comprises:

a level sensor (not shown) disposed on the top of the culture dish 24;

and a control mechanism (not shown in the figure) which is electrically connected with the liquid inlet pump 13 and the liquid level sensor respectively and is used for controlling the liquid inlet pump 13 to stop working when the liquid level of the culture solution in the culture dish 24 reaches a preset height.

In this embodiment, in addition to the monitoring of the liquid level in the culture dish 24 by the overflow prevention pump 15, the monitoring of the liquid level in the culture dish 24 can be realized by providing a liquid level sensor in the culture dish 24.

In another embodiment of the present invention, the full circulation apparatus further comprises:

a level sensor (not shown) disposed on the top of the culture dish 24;

and a control mechanism (not shown in the figure) electrically connected with the liquid inlet pump 13 and the liquid level sensor respectively and used for controlling the liquid outlet pump 14 to start working when the liquid level of the culture liquid in the culture dish 24 reaches a preset height.

In this embodiment, in addition to the monitoring of the liquid level in the culture dish 24 by the overflow prevention pump 15, the monitoring of the liquid level in the culture dish 24 can be realized by providing a liquid level sensor in the culture dish 24.

It should be noted that, if the culture dish 24 is small in size, the liquid level sensor is difficult to be arranged on the top of the culture dish 24, so that it is more effective and reasonable to use the overflow prevention pump 15 to indirectly measure the liquid level of the culture solution in the culture dish 24 to reach the preset height.

Referring to fig. 3 and 4, the apparatus for circulating the culture solution of the spine is used for circulating the culture solution to the culture dish 24 disposed on the force loading mechanism, so as to avoid the problem that the culture solution may be contaminated by using a syringe in the related art. Wherein, when utilizing the complete circulating device of the culture solution of backbone to carry out the circulation of culture solution to culture dish 24, can utilize feed liquor pump 13 will be located the culture solution in storage container 121 and carry the inlet 241 of culture dish 24, utilize out liquid pump 14 to carry the culture solution in culture dish 24 to storage container 121 through liquid outlet 242 simultaneously, can also utilize anti-overflow pump 15 will reach the culture solution of the predetermined height of culture dish 24 and carry outside waste liquid case 19 through anti-overflow mouth 243. In some embodiments, the liquid inlet 241 and the liquid outlet 242 are located at the bottom of the culture dish 24, and the overflow prevention port 243 is located at the top of the culture dish 24.

Referring to FIG. 12, in one embodiment of the present invention, the culture dish 24 is disposed on a fixing stand 249, and the fixing stand 249 is fixed on the fourth plate 215, so as to facilitate the cleaning of the fixing stand 249. Since the culture dish 24 is typically made of glass, in order to prevent the bottom of the culture dish 24 from being broken when the axial loading device is used for axially loading the spine, in some embodiments, a silicone pad 248 may be disposed at the bottom of the culture dish 24 and the fixing table 249.

The force loading mechanism is described below with reference to the drawings.

Referring to fig. 5, 7 and 10, the force loading mechanism of the spine includes an axial loading device and a circumferential loading device of the spine, wherein the axial loading device and the circumferential loading device share a frame 21 portion of the force loading mechanism.

In one embodiment of the present invention, the force loading mechanism of the spine comprises a frame 21, an axial loading mechanism 22 and a circumferential loading mechanism 23, wherein:

the frame body 21 comprises two guide posts 211 extending along the axial direction of the spine, and a first flat plate 212, a second flat plate 213, a third flat plate 214 and a fourth flat plate 215 which are sequentially arranged from top to bottom along the axial direction of the spine, wherein the first flat plate 212 and the fourth flat plate 215 are both fixed with the guide posts 211, the second flat plate 213 and the third flat plate 214 are fixedly connected, and the second flat plate 213 and the third flat plate 214 can both move up or down along the guide posts 211;

the axial loading mechanism 22 is fixed to the first plate 212;

the circumferential loading mechanism 23 is disposed between the second flat plate 213 and the third flat plate 214;

the frame body 21 is provided with a culture dish 24 with an opening, the culture dish 24 is used for accommodating a spine, the upper end and the lower end of the spine are respectively fixed on a first mounting seat 25 and a second mounting seat 26, the first mounting seat 25 is rotationally connected with a third flat plate 214, the first mounting seat 25 can rotate along the circumferential direction of the spine, and the second mounting seat 26 is fixed with a fourth flat plate 215;

the axial loading mechanism 22 is used for applying a force along the axial direction of the spine to the first mounting seat 25 so as to axially load the spine;

the circumferential loading mechanism 23 is used to apply a force to the first mount 25 in the circumferential direction of the spine to circumferentially load the spine.

In this embodiment, by providing the axial loading mechanism 22 on the first flat plate 212, a force along the axial direction of the spine can be applied to the first mounting seat 25 to axially load the spine; by providing a circumferential loading mechanism 23 between the second plate 213 and the third plate 214, it is possible to apply a force to the first mounting block 25 in the circumferential direction of the spine to circumferentially load the spine. Thus, the above described solution allows to study the overall mechanical properties of the spine.

In the related art, the spine is generally used as a subject to be studied for the study of mechanical properties. For example, patent publication No. CN113057595A discloses an in vivo loading device for spinal motion segments, which axially loads the spine by means of compression springs. For another example, patent publication No. CN214572028U discloses an in vitro culture loading device for an angle-adjustable spinal motion segment, which simulates the natural flexion and extension angles of a cervical vertebral motion unit and studies the influence of different flexion and extension angles and different acting forces (i.e., lateral loading) on the cervical intervertebral disc.

For patients with lumbar diseases, rotational reduction of lumbar vertebrae in sitting position (see patent publication No. CN 204033550U) is one of the commonly used methods of chinese medicine treatment. That is, the rotary lumbar reduction requires the patient to sit on a chair, the doctor to sit on another chair behind the patient, and an assistant to fix the lower limbs of the patient, and the doctor and the assistant cooperate to complete the operation of the method. In this context, the above-mentioned related art obviously fails to study the change of the spine under different rotational forces (i.e., circumferential loading).

However, according to the technical solution provided by the present invention, by providing the circumferential loading mechanism 23 on the frame 21, the acting force along the circumferential direction of the spine can be applied to the first mounting seat 25 to circumferentially load the spine, so that the variation of the spine under different rotational acting force loads can be studied.

In one embodiment of the present invention, two fifth flat plates 216 are fixed between the second flat plate 213 and the third flat plate 214, the fifth flat plate 216 is perpendicular to the second flat plate 213 or the third flat plate 214, and the circumferential loading mechanism 23 is fixed to one of the fifth flat plates 216.

In this embodiment, by fixing the two fifth flat plates 216 between the second flat plate 213 and the third flat plate 214, not only the fixed connection of the second flat plate 213 and the third flat plate 214 can be realized, but also the stability of the up and down movement of the second flat plate 213 and the third flat plate 214 along the guide post 211 can be ensured. Of course, the fixed connection between the second plate 213 and the third plate 214 can be realized by other methods, which are not limited herein.

It should be noted that the second flat plate 213 and the third flat plate 214 are provided for mounting the circumferential loading mechanism 23, and if only axial loading on the spinal column is considered, the second flat plate 213 may be omitted, i.e. the first output shaft 221 of the axial loading mechanism 22 may be directly connected with the third flat plate 214 to achieve compression and tension on the third flat plate 214.

Further, the axial urging mechanism 22 is located above the circumferential urging mechanism 23 in the height direction, and is not simply provided in view of the compactness of the overall structure of the force urging mechanism.

In addition to the two fifth flat plates 216 provided on the second flat plate 213 and the third flat plate 214, in order to further consider the compactness of the overall structure, the circumferential loading mechanism 23 is fixed to one of the fifth flat plates 216, instead of selectively fixing the circumferential loading mechanism 23 to the second flat plate 213 or the third flat plate 214.

In an embodiment of the present invention, the axial loading mechanism 22 includes a first output shaft 221, the first output shaft 221 is located on an axial center line of the spine, and the first output shaft 221 can extend and contract up and down along the axial direction of the spine to drive the first mounting seat 25 to move up and down by the extension and contraction of the first output shaft 221;

the circumferential loading mechanism 23 includes a second output shaft 231, and the second output shaft 231 can be extended and retracted back and forth along a direction perpendicular to the axial direction of the spine, so that the first mounting seat 25 is driven to rotate by the extension and retraction of the second output shaft 231.

In the present embodiment, by providing the first output shaft 221 and the second output shaft 231, it is possible to conveniently achieve the axial loading of the first mounting seat 25 by the axial loading mechanism 22 along the spinal column and the circumferential loading of the first mounting seat 25 by the circumferential loading mechanism 23 along the spinal column.

Of course, the axial loading mechanism 22 may not include the first output shaft 221, and the circumferential loading mechanism 23 may not include the second output shaft 231, which is not limited herein. For example, by manually controlling the axial loading and circumferential rotation of the first mount 25, and then fixed in position.

In addition, the first output shaft 221 is located on the axial center line of the spine, so that the axial loading effect on the spine can be ensured.

Of course, the first output shaft 221 may not be located on the axial center line of the spine, and is not particularly limited herein.

In one embodiment of the present invention, the axial loading mechanism 22 and the circumferential loading mechanism 23 are both linear servo motors, so that the axial loading control and the circumferential loading control of the spine can be conveniently realized.

Of course, the axial loading mechanism 22 and the circumferential loading mechanism 23 may be hydraulic mechanisms or pneumatic mechanisms, and the specific types of the axial loading mechanism 22 and the circumferential loading mechanism 23 are not limited herein.

In one embodiment of the present invention, the axial loading means further comprises a first connector 222, the first connector 222 being threadedly secured to the first output shaft 221 and the first force sensor 223, respectively.

Since the axial loading mechanism 22 and the first force sensor 223 are standard components, they generally cannot be directly fixedly connected, and in order to facilitate the fixed connection of the two, they can be fixedly screwed to the first output shaft 221 and the first force sensor 223 by means of the first connecting member 222.

Of course, the first connection member 222 may not be provided, that is, the first output shaft 221 and the first force sensor 223 may be directly fixed by a secondary processing method (for example, the end portion of the first output shaft 221 and the first force sensor 223 are processed with a thread structure capable of matching with each other), which is not limited herein.

Referring to fig. 6, 8 and 11, in an embodiment of the present invention, the force loading mechanism further includes a rotating assembly 232, the rotating assembly 232 includes a rotating shaft 232a, a fixing seat 232b and a bearing 232c, the rotating shaft 232a is disposed through the fixing seat 232b and the bearing 232c, the fixing seat 232b fixes the bearing 232c on the third flat plate 214, an upper end of the rotating shaft 232a is movably connected to the second output shaft 231, and a lower end of the rotating shaft 232a is fixed to the first mounting seat 25.

In the present embodiment, the rotation assembly 232 is provided to achieve the rotation connection between the first mounting seat 25 and the third plate 214.

Of course, the rotational connection between the first mounting seat 25 and the third plate 214 may be other manners, and is not limited herein.

With continued reference to fig. 6, 8, and 11, in an embodiment of the present invention, the force loading mechanism further includes a connecting component 233, the connecting component 233 includes a second connecting component 233a, a third connecting component 233b, and a fourth connecting component 233c, which are sequentially connected, one end of the second connecting component 233a is fixed to the second output shaft 231, the other end is rotatably connected to the third connecting component 233b, the third connecting component 233b is slidably connected to the fourth connecting component 233c, the fourth connecting component 233c is fixedly connected to the rotating shaft 232a, and an axial direction of the fourth connecting component 233c is perpendicular to an axial direction of the rotating shaft 232 a.

In the present embodiment, by providing the connection member 233, the connection member 233 can be made to have a rotational degree of freedom and a sliding degree of freedom, so that the rotary shaft 232a can swing back and forth in its circumferential direction.

Of course, the sliding freedom may be omitted, that is, the connection assembly 233 includes only the second connection member 233a and the fourth connection member 233c connected in sequence, one end of the second connection member 233a is fixed to the second output shaft 231, the other end is rotatably connected to the fourth connection member 233c, the fourth connection member 233c is fixedly connected to the rotation shaft 232a, and the axial direction of the fourth connection member 233c is perpendicular to the axial direction of the rotation shaft 232 a. Compared with the scheme, the scheme with the omitted sliding freedom degree has small swing amplitude and is not beneficial to realizing the circumferential loading effect on the spine, namely the scheme with the rotating freedom degree and the sliding freedom degree can realize the better circumferential loading effect on the spine.

In the related art, the spine is generally used as a research object to research the mechanical correlation, and some previous patents of the inventor disclose technical solutions for applying axial stress (i.e. performing axial loading) to the spine.

For example, patent publication No. CN113057595A discloses an in vivo loading device for spinal motion segments, which axially loads the spine by means of compression springs. However, this solution makes it difficult to achieve a continuous constant force loading of the spine in the axial direction when creep of the spine occurs.

For another example, patent publication No. CN109468360A discloses a tension-compression integrated loading device for spinal motion segments, which loads the spine axially by mounting weights. Although the scheme can realize the continuous constant force loading on the spine in the axial direction, the loading mode of the mode is discrete loading (namely, the staged constant force loading is realized by replacing weights with different masses), and the continuous variable force loading cannot be realized, namely, the influence caused by creep deformation cannot be effectively eliminated.

Further, since the foot part continuously applies axial and continuous variable force to the spine during walking, it is necessary to improve the axial loading device of the spine in order to research more mechanical characteristics of the whole spine.

In order to solve the technical problem, the inventor discovers in the development process that: the axial loading mechanism 22, the first force sensor 223 and the control mechanism cooperate with each other to realize axial continuous constant force loading and axial continuous variable force loading on the spine, so that the influence caused by creep can be effectively eliminated. That is, an axial control algorithm is added, rather than simply manually adjusting the axial pressure (e.g., replacing a weight of a different mass).

Referring to fig. 7 and 9, in an embodiment of the present invention, the force loading mechanism further includes:

a first force sensor 223 having one end fixed to the first output shaft 221 and the other end fixed to the second plate 213;

a second force sensor 234 having one end fixed to the second output shaft 231 and the other end fixed to the second link 233 a;

control means (not shown in the drawings) electrically connected to the axial loading means 22, the circumferential loading means 23, the first force sensor 223 and the second force sensor 234, respectively;

the control mechanism controls the axial loading mechanism 22 to carry out axial continuous constant force loading and axial continuous variable force loading on the spine;

the circumferential loading mechanism 23 is controlled by the control mechanism to carry out circumferential continuous constant force loading and circumferential continuous variable force loading on the spine.

In the embodiment, the axial loading mechanism 22, the first force sensor 223 and the control mechanism cooperate to jointly realize axial continuous constant force loading and axial continuous variable force loading on the spine, so that the influence caused by creep can be effectively eliminated; through the cooperation of the circumferential loading mechanism 23, the second force sensor 234 and the control mechanism, the circumferential continuous constant force loading and the circumferential continuous variable force loading on the spine are realized together, so that the change of the spine under different rotation acting force loads can be researched.

It can be understood that the chip of the control mechanism is preset with an associated axial control algorithm and a circumferential control algorithm, and the position offset of the first output shaft 221 and the second output shaft 231 is adaptively changed by acquiring the current acting force detected by the first force sensor 223 and the second force sensor 234, so that the axial continuous constant force loading and the axial continuous variable force loading as well as the circumferential continuous constant force loading and the circumferential continuous variable force loading on the spine can be realized.

When the axial and circumferential loading of the spine reaches a certain time, the spine may creep, and at this time, the offset of the positions of the first output shaft 221 and the second output shaft 231 needs to be adaptively changed by acquiring the current acting force detected by the first force sensor 223 and the second force sensor 234, so as to ensure the axial loading and circumferential loading effect on the spine. However, it should be noted that, since the force information may be distorted due to the loading environment, the state of the spine, and other factors, the data collected by the first force sensor 223 and the second force sensor 234 needs to be filtered online to achieve the purpose of reducing the influence of the environmental noise, so as to obtain the true contact force information.

In some embodiments, the present invention may use kalman filter based force sensing information filtering to perform the estimation of the force signal. Kalman filtering is a method for optimally estimating the state of a system from linear system state equations. Because the estimation process is realized in an iterative calculation mode, only process noise, measurement noise and the system state at the current moment need to be considered in the estimation process, and integrally collected data does not need to be stored, so that the method is suitable for the requirement of acquiring force sensing information in real time in the research.

The following describes the axial control algorithm and the circumferential control algorithm of the control mechanism.

In one embodiment of the invention, the control mechanism is configured to perform the following operations:

s11, acquiring a current acting force detected by the first force sensor 223 in the current period;

s12, determining the theoretical position of the first output shaft 221 in the current period based on the theoretical acting force in the current period and a preset coefficient;

s13, keeping the theoretical position unchanged to realize axial continuous constant force loading;

s14, obtaining a position difference value of the current period based on the coefficient and the difference value of the current acting force and the theoretical acting force of the current period;

s15, correcting the theoretical position based on the position difference value to correct the theoretical acting force to the current acting force;

and S16, taking the current acting force as the theoretical acting force of the next period, and executing the steps S11, S12, S14 and S15, thereby realizing axial continuous variable force loading.

In the present embodiment, the axial loading mechanism 22 (e.g., the motor) can be simplified to a spring model, i.e., F = kx, where F is an elastic force (i.e., a force in the present embodiment), k is an elastic coefficient (i.e., a coefficient in the present embodiment), and x is a deformation amount (i.e., a position of the first output shaft 221 in the present embodiment). Therefore, by means of a preset theoretical acting force and a preset coefficient, a theoretical position of the first output shaft 221 can be obtained, and the theoretical position is kept unchanged, so that axial continuous constant force loading is realized; through the current acting force detected by the first force sensor 223, the preset theoretical acting force and the preset coefficient, a position difference value expected to be corrected at the current circumference of the first output shaft 221 can be obtained, so that the theoretical position of the first output shaft 221 can be corrected based on the position difference value to correct the theoretical acting force to the current acting force, and therefore axial continuous variable force loading is achieved.

For example, the current acting force is 9.7N, the theoretical acting force is 10N, and the current acting force detected by the first force sensor 223 can be corrected to 10N by the above axial control algorithm, so as to implement axial continuous constant force loading; for another example, the current acting force is 10.3N, the theoretical acting force is 10N, and the current acting force detected by the first force sensor 223 can be corrected to 10N by the above-mentioned axial control algorithm, so as to implement the axial continuous constant force loading.

For example, the current acting force is 9.7N, the theoretical acting force is 10N, and the current acting force detected by the first force sensor 223 can be corrected to 9.7N by the above axial control algorithm, so as to realize the axial continuous variable force loading; for another example, the current acting force is 10.3N, the theoretical acting force is 10N, and the current acting force detected by the first force sensor 223 can be corrected to 10.3N by the above axial control algorithm, so as to realize axial continuous variable force loading.

In one embodiment of the invention, the control mechanism is configured to perform the following operations:

s21, acquiring the current acting force detected by the second force sensor 234 in the current period;

s22, determining the theoretical position of the second output shaft 231 in the current period based on the theoretical acting force in the current period and a preset coefficient;

s23, keeping the theoretical position unchanged to realize circumferential continuous constant force loading;

s24, obtaining a position difference value of the current period based on the coefficient and the difference value of the current acting force and the theoretical acting force of the current period;

s25, correcting the theoretical position based on the position difference value to correct the theoretical acting force to the current acting force;

and S26, taking the current acting force as the theoretical acting force of the next period, and executing the steps S21, S22, S24 and S25, thereby realizing circumferential continuous variable force loading.

In the present embodiment, the circumferential loading mechanism 23 (e.g., a motor) can be simplified to a spring model, i.e., F = kx, where F is an elastic force (i.e., an acting force in the present embodiment), k is an elastic coefficient (i.e., a coefficient in the present embodiment), and x is a deformation amount (i.e., a position of the second output shaft 231 in the present embodiment). Therefore, by means of a preset theoretical acting force and a preset coefficient, a theoretical position of the second output shaft 231 can be obtained, and the theoretical position is kept unchanged, so that circumferential continuous constant force loading is realized; through the current acting force detected by the second force sensor 234, the preset theoretical acting force and the preset coefficient, a position difference value expected to be corrected at the current circumference of the second output shaft 231 can be obtained, so that the theoretical position of the second output shaft 231 can be corrected based on the position difference value to correct the theoretical acting force to the current acting force, and therefore circumferential continuous variable force loading is achieved.

For examples in the circumferential loading device, reference may be made to or by examples in the axial loading device, and details are not described here.

The fixing method may be a screw connection, or may be other fixing methods, and is not limited herein.

In addition, an embodiment of the present invention further provides a loading method for a spinal column, which is based on the loading system for a spinal column mentioned in any one of the above embodiments, and the method includes:

before the force loading mechanism performs a mechanical experiment on the spine, the culture solution in the storage container 121 is conveyed into the culture dish 24 through the liquid inlet pump 13;

after the mechanical experiment of the force loading mechanism on the spine is completed, the culture solution in the culture dish 24 is conveyed into the waste solution tank 19 through the liquid outlet pump 14; or, in the process of carrying out the mechanical experiment of the spine, the culture solution in the culture dish 24 is conveyed to the storage container 121 through the liquid outlet pump 14;

wherein, power loading mechanism carries out mechanics experiment to the backbone, includes:

when the force loading mechanism loads the spine axially, the axial loading mechanism 22 is controlled to apply acting force to the first mounting seat 25 along the axial direction of the spine so as to load the spine axially;

when the force loading mechanism loads the spine in the circumferential direction, the circumferential loading mechanism 23 is controlled to apply a force to the first mounting seat 25 in the circumferential direction of the spine so as to load the spine in the circumferential direction.

It should be noted that the method and the spinal loading system in the above embodiments are implemented based on the same inventive concept, so that the two have the same beneficial effects, and the beneficial effects of the using method are not described in detail herein.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one of 8230, and" comprising "does not exclude the presence of additional like elements in a process, method, article, or apparatus comprising the element.

Finally, it is to be noted that: the above description is only a preferred embodiment of the present invention, and is only used to illustrate the technical solutions of the present invention, and not to limit the protection scope of the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention shall fall within the protection scope of the present invention.

Claims (10)

1. A spinal loading system, comprising:

the culture box is internally provided with a force loading mechanism with spines, the force loading mechanism is provided with a culture dish with an opening, the culture dish is used for containing the spines, and the culture box is used for providing a constant-temperature and constant-humidity environment;

a storage box, in which a storage container for containing a culture solution is accommodated, for providing an environment of a storage temperature of the culture solution;

the two ends of the liquid inlet pump are respectively communicated with the storage container and the culture dish through pipelines and are used for conveying the culture solution in the storage container to the culture dish;

one end of the liquid outlet pump is communicated with the bottom of the culture dish through a pipeline, and the other end of the liquid outlet pump is communicated with an external waste liquid box or the storage container through a pipeline and is used for conveying the culture liquid in the culture dish into the waste liquid box or the storage container;

the force loading mechanism comprises:

the frame body comprises two guide pillars extending along the axial direction of a spine, and a first flat plate, a second flat plate, a third flat plate and a fourth flat plate which are sequentially arranged from top to bottom along the axial direction of the spine, wherein the first flat plate and the fourth flat plate are both fixed with the guide pillars, the second flat plate and the third flat plate are fixedly connected, and the second flat plate and the third flat plate can both move upwards or downwards along the guide pillars;

the axial loading mechanism is fixed on the first flat plate;

a circumferential loading mechanism disposed between the second plate and the third plate;

the upper end and the lower end of the spine are respectively fixed on a first mounting seat and a second mounting seat, the first mounting seat is rotatably connected with the third flat plate, the first mounting seat can rotate along the circumferential direction of the spine, and the second mounting seat is fixed with the fourth flat plate;

the axial loading mechanism is used for applying acting force along the axial direction of the spine to the first mounting seat so as to axially load the spine;

the circumferential loading mechanism is used for applying acting force along the circumferential direction of the spine to the first mounting seat so as to circumferentially load the spine.

2. The spinal loading system of claim 1, wherein two fifth plates are secured between the third plate and the fourth plate, the fifth plates being perpendicular to the third plate or the fourth plate, the circumferential loading mechanism being secured to one of the fifth plates.

3. The spinal loading system according to claim 1, wherein the axial loading mechanism comprises a first output shaft, the first output shaft is located on an axial center line of the spinal column, and the first output shaft can be extended and retracted up and down along the axial direction of the spinal column so as to drive the first mounting seat to move up and down through the extension and retraction of the first output shaft;

the circumferential loading mechanism comprises a second output shaft which can stretch back and forth along the direction perpendicular to the axial direction of the spine so as to drive the first mounting seat to rotate through the stretching of the second output shaft.

4. The spinal loading system of claim 3, wherein the axial loading mechanism and the circumferential loading mechanism are both linear servomotors.

5. The spinal loading system of claim 1 wherein the incubator is vented with carbon dioxide gas.

6. The spinal loading system of claim 1, wherein the opening of the culture dish is provided with a splash cover, the splash cover being of a half-and-half design.

7. The spinal loading system according to claim 1, wherein the side wall of the culture dish is provided with a bone cement injection port for injecting bone cement into the culture dish at a position of the fixation spine to fix the spine.

8. A spinal loading system according to any one of claims 1 to 7, further comprising:

and the two ends of the anti-overflow pump are respectively communicated with the top of the culture dish and the waste liquid tank through pipelines, and the anti-overflow pump is used for conveying the culture liquid with the preset height of the culture dish into the waste liquid tank.

9. A spinal loading system according to any one of claims 1 to 7, further comprising:

the liquid level sensor is arranged at the top of the culture dish;

and the control mechanism is respectively electrically connected with the liquid inlet pump, the liquid outlet pump and the liquid level sensor and is used for controlling the liquid inlet pump to stop working or controlling the liquid outlet pump to start working when the liquid level of the culture solution in the culture dish reaches a preset height.

10. A spinal loading method based on the spinal loading system of any one of claims 1-9, the method comprising:

before the force loading mechanism carries out mechanical experiment on the spine, the culture solution in the storage container is conveyed into the culture dish through the liquid inlet pump;

after the mechanical experiment of the force loading mechanism on the spine is completed, the culture solution in the culture dish is conveyed to the waste liquid tank through the liquid outlet pump; or, in the process of carrying out the mechanical experiment of the spine, the culture solution in the culture dish is conveyed into the storage container through the liquid outlet pump;

wherein the force loading mechanism performs mechanical experiments on the spine, comprising:

when the force loading mechanism carries out axial loading on the spine, the axial loading mechanism is controlled to apply acting force along the axial direction of the spine to the first installation seat so as to carry out axial loading on the spine;

when the force loading mechanism carries out circumferential loading on the spine, acting force along the circumferential direction of the spine is applied to the first mounting seat by controlling the circumferential loading mechanism so as to carry out circumferential loading on the spine.

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