CN115369024A - Gas intercommunication structure and card box - Google Patents

Abstract

The present invention relates to the field of biomedical technology. Embodiments of the present invention provide a gas communication structure and a cartridge. The second sealing film can seal the second gas tank so that the gas in the second gas tank is not separated from the outside world. The atmosphere is communicated, so that gas communication can be formed between the fifth air hole and the sixth air hole, and the air is pumped or inflated through the air pressure regulating device, so that the liquid in the cartridge can communicate between the chambers. Therefore, the following problems in the prior art are effectively solved: in the prior art, in the cartridge, the air pressure in the cartridge is adjusted by gas, so that the samples, reagents or liquids in the cartridge can flow , During this process, there is a technical problem of poor airtightness between the air pressure regulating device and the cartridge.

Description

A gas communication structure and cartridge

technical field

The invention relates to the field of biomedical technology, in particular to a gas communication structure and a cartridge.

Background technique

Nucleic acid detection plays a very important role in many fields of biochemical analysis, such as clinical medicine, forensic identification, genetic testing, etc., and has been widely used in biomedicine and other fields. In the prior art, the spin column method or magnetic bead method is usually used for nucleic acid extraction, coupled with subsequent detection steps such as nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biochips, so that the entire "from sample to result" Automated instrumentation is very difficult to implement. As far as the transfer of active ingredients in each step is concerned, manual transfer is often used in the prior art. Not only is the operation cumbersome, time-consuming and laborious, but it is also difficult to transfer the sample fully and efficiently. Manual operation can easily lead to unstable results. High difficulty.

The mainstream technology of molecular detection is fluorescent quantitative PCR technology. Since PCR technology has the characteristics of exponentially amplifying templates, the existing open consumables make the whole operation process easy to cause PCR aerosol pollution, which affects the purity of the extract and becomes a limitation of fluorescent quantitative PCR. An important factor for the further application of PCR technology in clinical practice.

In the prior art, in the cartridge, the air pressure in the cartridge is adjusted by gas, so that the sample, reagent or liquid in the cartridge can flow. During this process, the air pressure adjustment device and the cartridge There is a technical problem of poor airtightness between them.

Contents of the invention

The object of the present invention is: the present invention provides a gas communication structure and cartridge with simple structure and good sealing performance. , it can also realize that after the chamber is filled, the liquid is prevented from continuing to flow into the chamber, so as to process the liquid in the chamber, so as to meet the application of specific functional scenarios.

In order to achieve the above object, the present invention provides a gas communication structure, the gas communication structure includes:

second air tank;

The fifth air hole, the fifth air hole communicates with the second air groove and is used to connect the air pressure regulating device,

The sixth air hole, the sixth air hole communicates with the second air groove, and the sixth air hole is provided with a waterproof and breathable membrane I, which is used to communicate with the chamber that allows liquid to flow in;

The second air groove is provided with a second sealing film to form a second air chamber;

Wherein, the fifth air hole and the sixth air hole realize gas communication in the second air chamber.

In some embodiments of the present application, the waterproof and breathable membrane is welded to the second air groove around the sixth air hole. In order to facilitate the setting of the waterproof and breathable membrane, and to effectively prevent liquid from overflowing outside the second air groove, the waterproof and breathable membrane is welded to the second air groove by laser spot welding, and is located in the sixth air hole around.

In some embodiments of the present application, the fifth air hole is provided with a waterproof and breathable membrane II. The second waterproof and breathable membrane is to further prevent liquid from penetrating the first waterproof and breathable membrane from entering the fifth air hole from the sixth air hole.

In some embodiments of the present application, the first waterproof and breathable membrane and the second waterproof and breathable membrane are the same second waterproof and breathable membrane. The second waterproof and breathable membrane is attached to the second air groove, and is located above the fifth air hole and the sixth air hole, and is welded on the surface of the fifth air hole and the sixth air hole by laser spot welding , is convenient for processing and manufacturing, and can effectively ensure that the fifth air hole and the sixth air hole will not have liquid overflow.

In some embodiments of the present application, the second waterproof and breathable membrane covers the second air groove, and is welded to the second air groove around the fifth air hole and the sixth air hole.

The embodiment of the present application also provides a kind of card box, and described card box comprises:

The gas communication structure as described above;

Cartridge body;

Wherein, the second air groove, the fifth air hole and the sixth air hole are all arranged on the cartridge body, and the second sealing film is arranged on the surface of the cartridge body.

In some embodiments of the present application, the cartridge also includes:

The third air hole, the second air channel and the third air channel provided on the cartridge body;

Wherein, one end of the third air hole communicates with the air pressure regulating device; one end of the second air channel communicates with the other end of the third air hole; one end of the third air channel communicates with the second air channel. The other end is connected, and the other end of the third airway is connected to the fifth air hole;

The end of the third air hole communicating with the air pressure regulating device and the third air passage are located on the same surface of the cartridge body, and the second air passage and the third air passage are located on the same surface of the cartridge body. different sides of the body.

In some embodiments of the present application, the second air channel and the second air groove are located on the same surface of the cartridge body.

In some embodiments of the present application, the cartridge further includes a first sample mixing chamber provided on the cartridge body;

The first sample mixing chamber communicates with the sixth air hole; the first sample mixing chamber is provided with a first freeze-dried bead and a first mixing bead, and the first mixing bead moves the sample and the The first freeze-dried beads are mixed;

The first sample mixing chamber is also provided with a liquid inlet and a liquid outlet, the liquid inlet is connected to the second channel for the sample to enter the first sample mixing chamber, and the liquid outlet is connected to the second channel for the liquid to enter the first sample mixing chamber. The fourth channel of the output.

In some embodiments of the present application, the first freeze-dried beads include excipients, and the weight of the excipients accounts for 20%-60% of the total weight of the first freeze-dried beads.

Compared with the prior art, a gas communication structure according to the embodiment of the present invention has the following beneficial effects:

Embodiments of the present invention provide a gas communication structure with a simple structure and good sealing performance. The second sealing film can seal the second gas groove so that the gas in the second gas groove does not communicate with the outside atmosphere, thereby The gas communication can be formed between the fifth air hole and the sixth air hole, and the air can be pumped or inflated through the air pressure regulating device, so that the liquid can flow into or out of the chamber, and at the same time, under the action of the waterproof and breathable membrane one, the cavity can also be realized. After the chamber is filled, the liquid is prevented from continuing to flow into the chamber, so that the liquid in the chamber can be processed, so as to meet the application of specific functional scenarios.

Description of drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the drawings that need to be used in the embodiments or the description of the prior art. Obviously, the drawings in the following description are only the drawings of the present invention. For some embodiments, those of ordinary skill in the art can also obtain other drawings based on these drawings without paying creative efforts.

Fig. 1 is a schematic diagram of the axial side of a cartridge according to Embodiment 1 of the present invention;

Fig. 2 is the back view of a kind of cartridge of embodiment 1 of the present invention;

Fig. 3 is a positive exploded view of a cartridge according to Embodiment 1 of the present invention;

Figure 4 is an exploded view of a cartridge in Embodiment 1 of the present invention;

Fig. 5 is a schematic diagram of the forward structure of the cartridge body of a cartridge according to Embodiment 1 of the present invention;

Fig. 6 is a schematic structural view of the back of the cartridge body of a cartridge according to Embodiment 1 of the present invention;

Fig. 7 is a front structural schematic diagram of a cartridge body of a cartridge according to Embodiment 1 of the present invention;

8 is a schematic top view of a cartridge body of a cartridge according to Embodiment 1 of the present invention;

Fig. 9 is a sectional view of A-A in Fig. 8;

Fig. 10 is a structural schematic diagram of a siphon;

Figure 11 is a schematic front view of the first thimble valve;

Fig. 12 is a cross-sectional view of A-A in Fig. 11 in the non-working state of the first thimble valve;

Fig. 13 is a sectional view of A-A in Fig. 11 in the working state of the first thimble valve;

Fig. 14 is a schematic diagram of the front structure of a cartridge according to Embodiment 1 of the present invention;

Fig. 15 is a schematic structural view of the back side of a cartridge according to Embodiment 1 of the present invention.

In the picture:

100, cartridge body; 110, sample adding chamber; 111, sample adding chamber; 120, siphon tube; 121, siphon part; 1210, connecting part; 1211, first straight pipe part; 1214, the second bending part; 1215, the third straight pipe part; 1221, the inlet connection part; 1222, the outlet connection part; 130, the sample cracking chamber; 131, the baffle; 132, the sample cracking chamber; , sample filter chamber; 141, sample filter chamber; 151, first sample mixing chamber; 1511, first sample mixing chamber; 152, second sample mixing chamber; 1521, second sample mixing chamber; 1512, first ventilation Road; 1522, second airway;

161, the first PCR chamber; 1611, the first PCR chamber; 162, the second PCR chamber; 1621, the second PCR chamber; 170, the step; 1010, the first damping chamber; 1011, the first damping chamber; 1020, the second Two damping chambers; 1021, the second damping chamber;

201, the first air hole; 202, the second air hole; 203, the third air hole; 204, the fourth air hole; 205, the fifth air hole; 206, the sixth air hole; 207, the seventh air hole; 208, the eighth air hole; 209, Ninth air hole; 2010, tenth air hole; 210, first air channel; 211, first air channel; 220, second air channel; 221, second air channel; 230, third air channel; 231, third air channel 251, the first runner; 252, the second runner; 253, the third runner; 254, the fourth runner; 255, the fifth runner; 256, the sixth runner; 257, the seventh runner; 258, the eighth channel; 259, the ninth channel; 2510, the first channel; 2520, the second channel; 2530, the third channel; 2540, the fourth channel; 2550, the fifth channel; 2560, the sixth channel, 2570, the seventh channel; 2580, the eighth channel; 2590, the ninth channel;

300, box body sampling nozzle; 301, through hole; 302, slope surface; 310, elastic part;

400, cover; 401, steps;

510, the first thimble valve; 511, the valve cavity; 5111, the raised part; 5112, the flow hole; 5113, the opening; 512, the rubber pad; 520, the second thimble valve; 521, the second valve cavity; 522, the first Two silica gel pads; 530, the third thimble valve; 531, the third valve chamber; 532, the third silica gel pad; 540, the fourth thimble valve; 541, the fourth valve cavity; 542, the fourth silica gel pad; 550, the fifth Thimble valve; 551, fifth valve cavity; 552, fifth silicone pad; 560, sixth thimble valve; 561, sixth valve cavity; 562, sixth silicone pad; 570, seventh thimble valve; 571, seventh valve Cavity; 572, the seventh silica gel pad;

610, the first freeze-dried beads; 620, the second freeze-dried beads;

710, the first mixing beads; 720, the second mixing beads;

801, the first choke valve; 802, the second choke valve;

910, the first air chamber; 911, the first air chamber; 920, the second air chamber; 921, the second air chamber; 930, the third air chamber; 931, the third air chamber;

1200, the first sealing film; 1201, the main sealing film;

1300. The third sealing film;

1400. The second sealing film;

1510. The first waterproof and breathable membrane;

1520, the second waterproof and breathable membrane;

1530. The third waterproof and breathable membrane.

Detailed ways

The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the terms "upper", "lower", "left", "right", "top", "bottom" etc. Orientation or positional relationship is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation of the present invention.

It is to be understood that the terms "length", "width", "top", "bottom", "front", "rear", "left", "right", "vertical", "horizontal", "top" , "bottom", "inner", "outer" and other indicated orientations or positional relationships are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying No device or element must have a specific orientation, be constructed, and operate in a specific orientation and therefore should not be construed as limiting the invention.

In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be interpreted as indicating or implying relative importance or implicitly specifying the quantity of indicated technical features. Thus, a feature defined as "first" and "second" may explicitly or implicitly include one or more of these features. In the description of the present invention, "plurality" means two or more, unless otherwise specifically defined.

Example 1

Referring to Fig. 1, Fig. 1 is a schematic structural view of a cartridge according to Embodiment 1 of the present invention;

As shown in Figures 1 to 15, a cartridge according to a preferred embodiment of the present invention, as shown in Figures 1 to 4, includes a cartridge body 100, a first freeze-dried bead 610, a second freeze-dried bead 620, a A mixing bead 710, a second mixing bead 720, a filter membrane 1600, a first waterproof and breathable membrane 1510, a second waterproof and breathable membrane 1520, a third waterproof and breathable membrane 1530, a first sealing membrane 1200, a second sealing membrane 1400, The third sealing film 1300, the first silica gel pad, the second silica gel pad 522, the third silica gel pad 532, the fourth silica gel pad 542, the fifth silica gel pad 552, the sixth silica gel pad 562 and the seventh silica gel pad 572;

As shown in Fig. 5-Fig. 7, the cartridge body 100 is provided with a cover 400, a cartridge body sampling nozzle 300, a sample adding chamber 111, a siphon part 121, a sample cracking chamber 132, a first valve chamber, a sample filter chamber 141, Second valve chamber 521, first sample mixing chamber 1511, third valve chamber 531, second sample mixing chamber 1521, fourth valve chamber 541, fifth valve chamber 551, sixth valve chamber 561, seventh valve chamber 571 , the first PCR chamber 1611 and the second PCR chamber 1621;

Sample adding chamber 111, sample cracking chamber 132, first valve chamber, sample filter chamber 141, second valve chamber 521, first sample mixing chamber 1511, third valve chamber 531, second sample mixing chamber 1521, fourth valve chamber The cavity 541, the fifth valve cavity 551, the sixth valve cavity 561, the seventh valve cavity 571, the first PCR cavity 1611 and the second PCR cavity 1621 are all arranged on the first surface of the cartridge body 100, and all of them face the card. The first surface of the box body 100 is an open cavity. The siphon part 121 is a groove opening toward the first surface of the cartridge body 100 , and the surface of the cartridge body 100 away from the first surface of the cartridge body 100 is the second surface.

Wherein, the first PCR chamber 1611 and the second PCR chamber 1621 are through chambers;

The partial structure of the first surface of the cartridge body 100 is recessed downwards as a whole, and the downwardly recessed part forms a stepped portion 170, so that the first surface of the cartridge body 100 presents a ladder shape as a whole; the first PCR chamber 1611 and the second PCR chamber 1621 is provided on the stepped portion 170 .

The cover 400 is connected with the sampling nozzle 300 of the box body, and the cover 400 can be closed on the sampling nozzle 300 of the box body, so that the sampling nozzle 300 of the box body can be sealed to prevent foreign matter from entering the sample injection chamber through the sampling nozzle 300 of the box body 111, and maintain the air pressure of each chamber in the cartridge body 100. The cover 400 also plays a role in preventing the liquid from flowing out of the cartridge, thereby preventing the sample from seeping out of the cartridge, causing virus infection in the sample to inspectors or PCR amplification products to spread in the environment, causing detection accidents.

The box body sampling nozzle 300 is connected with the sample adding chamber 111, the sample adding chamber 111 is connected with one end of the siphon part 121, the sample cracking chamber 132 is connected with the other end of the siphon part 121, and the sample adding chamber 111 is connected with the siphon part 121. The sample cracking chamber 132 is connected;

The first valve chamber is arranged at the bottom of the sample cracking chamber 132 and communicates with the sample cracking chamber 132;

The bottom of the first valve cavity is provided with a first channel hole and three first protrusions 5111, and a first opening is provided on the side wall of the first valve cavity, and the first opening communicates with the sample lysis chamber, and the sample lysis chamber The sample flowing out of 132 flows into the first valve cavity through the first opening, and then flows out through the first flow channel hole. The three first protrusions 5111 are arranged around the first flow channel hole with equal intervals, and the sample can flow from the three first flow channel holes. The gaps between the protrusions 5111 flow into the first channel holes, and the bottom of the first silicone pads are in contact with the first protrusions 5111. When the first silica gel pads are squeezed, the first silica gel pads are deformed and blocked. Block the first channel hole so that the sample cannot flow into other chambers through the first channel hole.

The cartridge body 100 is provided with a first flow channel 251 between the first valve cavity and the sample filter cavity 141, and the first valve cavity and the sample filter cavity 141 are connected through the first flow channel 251, that is, the first flow channel 251 and the first flow channel hole The other end of the first flow channel 251 is connected with the sample filter cavity 141, and the filter membrane is arranged in the sample filter cavity 141, and the filter membrane is used to filter out macromolecular impurities; the sample cracked in the sample cracking cavity 132, It flows into the first valve cavity through the first opening, enters the first flow channel 251 through the first flow channel hole, and then flows into the sample filter cavity 141; when the first silica gel pad is squeezed, the first silica gel pad is deformed and blocked. The first flow channel hole prevents the sample from flowing into the first flow channel 251 through the first flow channel hole, so that the sample cannot reach the sample filter cavity 141 .

The bottom of the second valve cavity 521 is provided with a second channel hole and three second protrusions, and a second opening is opened on the side wall of the second valve cavity 521, and the second opening communicates with the first sample mixing cavity, The sample flowing out of the sample filter cavity 141 flows out through the second flow channel hole, and the three second protrusions are arranged around the second flow channel hole with equal intervals, and the sample can flow into the space from the gap between the three second protrusions. In the second channel hole, the bottom of the second silica gel pad 522 is in contact with the second protrusion;

When the second silica gel pad 522 is squeezed, the second silica gel pad 522 is deformed and blocks the second channel hole, so that the sample cannot flow into other chambers through the second channel hole.

The first mixing beads 710 and the first freeze-dried beads 610 are located in the first sample mixing chamber 1511, driven by an external power device (such as a magnetic device), the first mixing beads 710 can be placed in the first sample mixing chamber 1511, on the one hand, the movement of the first mixing beads 710 can collide with the first freeze-dried beads 610, smashing the first freeze-dried beads 610, and accelerating the reconstitution of the first freeze-dried beads 610; on the other hand, The movement of the first mixing beads 710 makes the reconstituted first freeze-dried beads 610 directly and fully mix with the sample.

The cartridge body 100 is provided with a second flow channel 252 between the sample filter cavity 141 and the first sample mixing cavity 1511, and the second valve cavity 521 is arranged on the second flow channel 252, specifically: The first sample mixing chamber 1511 communicates with the second opening of the second valve chamber 521, and the second channel hole of the second valve chamber 521 communicates with the sample filter chamber 141 through the second flow channel 252, and the inside of the sample filter chamber 141 The filtered sample flows into the first sample mixing cavity 1511 through the second channel 252 through the second channel hole; when the second silica gel pad 522 is squeezed, the second silica gel pad 522 is deformed and blocks the first sample The second channel hole prevents the sample from flowing into the first sample mixing chamber 1511 through the second channel hole.

The bottom of the third valve cavity 531 is provided with a third channel hole and three third protrusions, the side wall of the third valve cavity 531 is provided with a third opening, the third opening communicates with the second sample mixing chamber, and the sample The sample flowing out of the filter cavity 141 flows into the next cavity through the third flow channel hole, and the three third protrusions are arranged around the third flow channel hole with equal intervals, and the sample can pass between the three third protrusions. The gap between the gaps flows into the third channel hole, and the bottom of the third silica gel pad 532 is in contact with the third protrusion;

When the third silica gel pad 532 is squeezed, the third silica gel pad 532 is deformed and blocks the third channel hole, so that the sample cannot flow into other chambers through the third channel hole.

The second mixing beads 720 and the second freeze-dried beads 620 are located in the second sample mixing chamber 1521. Driven by an external magnetic device, the second mixing beads 720 can move in the second sample mixing chamber 1521. On the one hand, The movement of the second mixing beads 720 can collide with the second freeze-drying beads 620, and the second freeze-drying beads 620 are smashed to accelerate the redissolving of the second freeze-drying beads 620. On the other hand, the second mixing beads 720 move The reconstituted second freeze-dried beads 620 are directly and thoroughly mixed with the sample.

The cartridge body 100 is provided with a third flow channel 253 between the sample filter chamber 141 and the second sample mixing chamber 1521, and the third valve chamber 531 is arranged on the third flow channel 253, specifically: the first The second sample mixing chamber 1521 communicates with the third opening of the third valve chamber 531, and the third channel hole of the third valve chamber 531 communicates with the sample filter chamber 141 through the third flow channel 253. After filtering in the sample filter chamber 141 The sample flows through the third flow channel 253 and enters the second sample mixing chamber 1521 through the third flow channel hole; when the third silica gel pad 532 is squeezed, the third silica gel pad 532 is deformed and blocks the third flow channel hole, so that the sample cannot flow into the second sample mixing chamber 1521 through the third channel hole.

Wherein, the second channel 252 and the third channel 253 communicate with each other. The second flow channel 252 and the third flow channel 253 can also be a flow channel whose first end is connected to the sample filter cavity 141. The cavity 1511 and the third end of the flow channel are realized by communicating with the second sample mixing cavity 1521 through the third valve cavity.

The bottom of the fourth valve cavity 541 is provided with a fourth channel hole and three fourth protrusions, and a fourth opening is opened on the side wall of the fourth valve cavity 541, and the fourth opening communicates with the first sample mixing cavity. The sample flowing out of the first sample mixing chamber 1511 flows into the next chamber through the fourth flow channel hole, and the three fourth protrusions are arranged around the fourth flow channel hole with equal intervals, and the sample can pass through the three fourth protrusions. The gap between the rising parts flows into the fourth channel hole, and the bottom of the fourth silica gel pad 542 is in contact with the fourth protrusion; when the fourth silica gel pad 542 is squeezed, the fourth silica gel pad 542 is deformed and blocked. Block the fourth channel hole so that the sample cannot flow into other chambers through the fourth channel hole.

The cartridge body 100 is provided with a fourth flow channel 254 between the first sample mixing chamber 1511 and the first PCR chamber 1611, the first sample mixing chamber 1511 communicates with one end of the fourth flow channel 254, and the first PCR chamber 1611 communicates with the other end of the fourth flow channel 254 , and the sample in the first sample mixing chamber 1511 flows into the first PCR chamber through the fourth flow channel 254 . The fourth valve chamber 541 is arranged on the fourth flow channel 254, and the fourth flow channel hole is in communication with the fourth flow channel 254; the sample mixed in the first sample mixing chamber 1511 flows into the first PCR through the fourth flow channel 254 inside cavity 1611;

When the fourth silica gel pad 542 is squeezed, the fourth silica gel pad 542 is deformed and blocks the fourth channel hole, so that the sample coming out of the first sample mixing chamber 1511 cannot flow into the first PCR through the fourth channel hole. Inside cavity 1611.

The bottom of the fifth valve chamber 551 is provided with a fifth channel hole and three fifth protrusions, and a fifth opening is opened on the side wall of the second valve chamber 521, and the fifth opening communicates with the second sample mixing chamber. The sample flowing out of the second sample mixing chamber 1521 flows into the next chamber through the fifth flow channel hole, and the three fifth protrusions are arranged around the fifth flow channel hole with equal intervals, and the sample can pass through the three fifth protrusions. The gap between them flows into the hole of the fifth flow channel, and the bottom of the fifth silicone pad is in contact with the fifth raised part; when the fifth silicone pad is squeezed, the fifth silicone pad is deformed and blocks the fifth flow channel hole, so that the sample cannot flow into other chambers through the fifth channel hole.

The cartridge body 100 is provided with a fifth flow channel 255 between the second sample mixing chamber 1521 and the second PCR chamber 1621, the second sample mixing chamber 1521 communicates with one end of the fifth flow channel 255, and the second PCR chamber 1621 communicates with one end of the fifth flow channel 255. The other end of the fifth flow channel 255 is connected, and the sample in the second sample mixing chamber 1521 flows into the second PCR chamber through the fifth flow channel 255 . The fifth valve chamber 551 is arranged on the fifth flow channel 255, and the fifth flow channel hole communicates with the fifth flow channel 255; the sample mixed in the second sample mixing chamber 1521 flows into the second PCR chamber through the fifth flow channel 255 within 1621;

When the fifth silica gel pad is squeezed, the fifth silica gel pad is deformed and blocks the fifth channel hole, so that the sample coming out of the second sample mixing chamber 1521 cannot flow into the second PCR chamber 1621 through the fifth channel hole .

The cartridge body 100 is provided with a first air groove 911, a first air hole 201, a second air hole 202 and a first air path 211, the first air hole 201 is arranged in the first air groove 911, and the second air hole 202 is arranged in the first air hole 202. On the gas groove 911 and the first gas path 211, the second gas hole 202 communicates with the first gas groove 911 and the first gas path 211, the first gas path 211 communicates with the upper part of the sample cracking chamber 132, and the first gas hole 201 is used for connecting Inflatable device or air pressure regulating device;

The first waterproof and gas-permeable membrane 1510 covers the first air groove 911 and is used to prevent the sample from leaking out of the first air groove 911 and to discharge the air in the sample. The first waterproof and breathable membrane 1510 is welded on the first air groove 911 by laser welding. In one embodiment, the first waterproof and gas-permeable membrane 1510 is welded on the groove wall of the first air groove 911 , and may also be welded on the surface of the top outer edge of the first air groove 911 . In another preferred embodiment, the first waterproof breathable membrane 1510 is welded around the first air hole 201, the second air hole 202, the ninth air hole 209, and the tenth air hole 2010 by laser welding; Welding near the outer periphery of the first air hole 201 can prevent the sample from seeping out or blocking the first air hole 201, so that the first air hole 201 cannot perform the operation of air intake or air outlet; The sample overflowing in the medium enters in the second air hole 202, even enters in the first air hole 201, causes the virus in the sample to leak, causes the problem of the medical accident of personnel infection; Welding on the ninth air hole 209 and the tenth air hole 2010 in order to make When a liquid sample enters the ninth air hole 209 or the tenth air hole 2010, it can be quickly blocked by the first waterproof gas-permeable membrane 1510, so that the sample stops entering the first PCR chamber or the second PCR chamber, and the first The waterproof breathable membrane 1510 is welded around the ninth air hole 209 and the tenth air hole, which can effectively increase the contact between the first waterproof breathable membrane 1510 and the ninth air hole 209 and the tenth air hole 2010. Being in contact with the first waterproof and gas-permeable membrane 1510 prevents the sample from entering the first air groove 911 and easily causing pollution events. Or directly weld a waterproof and breathable membrane separately around the ninth air hole 209, that is, the waterproof and breathable membrane is directly blocked on the ninth air hole 209, and another waterproof and breathable membrane is welded separately around the tenth air hole 2010, the waterproof and breathable membrane Directly blocking the tenth air hole 2010 can also realize the above functions.

In addition, the ninth air hole 209 and the tenth air hole 2010 can be set together in an independent air groove, and the air groove is covered and blocked by a waterproof breathable film, or the ninth air hole 209 is independently arranged in an air groove, The tenth air holes 2010 are independently arranged in an air groove, and are respectively covered and blocked by corresponding waterproof and breathable membranes. The first air hole 201 and the second air hole 202 are arranged in the same air groove to realize communication. At the same time, to realize communication between the first air hole 201 and the second air hole 202, a sealing film needs to be set on the first air groove for sealing, so that the air pump When the first air hole 201 is pumped or inflated, it can act on the sample lysis chamber 132 through the second air hole 202 .

In this embodiment, a second sealing film is provided on the outside of the first waterproof and gas-permeable film 1510 to seal the first air groove. When the first air hole 201 blows air in the external inflator, the air enters the first air groove 911 from the first air hole 201 , and then enters the first air path 211 through the second air hole 202 from the first air groove 911 , enter the sample cracking chamber 132 through the first air path 211, when the sample cracking chamber 132 is in a sealed state, the air entering the sample cracking chamber 132 from the first air hole 201 increases the air pressure in the sample cracking chamber, The sample in the sample cracking chamber 132 can be pressed into the sample filter chamber 141 from the sample cracking chamber 132 through the first valve chamber;

When the first air hole 201 is pumped by the external air pressure regulating device, the air in the sample cracking chamber 132 enters the first air groove 911 from the first air path 211 through the second air hole 202, and then passes through the first air groove 911. Withdrawing, since the air in the sample lysis chamber 132 is drawn out, the air pressure in the sample lysis chamber is reduced, so that the sample in the siphon part 121 or the sample addition chamber 111 is sucked into the sample lysis chamber 132 .

The cartridge body 100 is provided with a second air groove 921, a third air groove 931, a third air hole 203, a fourth air hole 204, a fifth air hole 205, a sixth air hole 206, a seventh air hole 207, an eighth air hole 208, a The second gas path 221 and the third gas path 231.

One end of the third air hole 203 communicates with the air pressure regulating device (such as an air pump) for blowing in air or extracting air, and the other end of the third air hole 203 communicates with the third air path 231 through the second air path 221, and the third air hole 203 One end communicated with the air pressure regulator and the third air path 231 are all located on the same face of the cartridge body 100, and the second air path 221 and the third air path 231 are positioned on the opposite face of the cartridge body 100; Gas path 221, and the second gas path 221 is located on the face opposite to the end that communicates with the third gas path 231 and the third air hole 203 with the air pressure regulator, the reasons are as follows:

1) When the air pump is directly connected to the third air circuit, it cannot be effectively sealed, and the air tightness requirements of the air pump cannot be guaranteed;

2) The periphery of the third gas path needs to be welded to achieve sealing, so there is a laser welding line, which will produce a raised feature with a height of 0.001-0.1mm on the cartridge body 100, and the plane where the cartridge body 100 is connected to the air pump Good flatness is required to ensure the airtightness of the contact, therefore, if the air pump is connected to the third air circuit, the seal with the air pump cannot be achieved, but through the second air circuit, the cartridge can be sealed with the air pump .

The second air path 221 communicates with the third air path 231 through the fourth air hole 204. Since the second air path and the third air path are not located on the same side of the cartridge body 100, the second air path is communicated with the second air path through the fourth air hole 204. The passage 221 and the third air passage 231 , so that air can flow from the fourth air hole 204 between the second air passage 221 and the third air passage 231 .

In this embodiment, one end of the third air hole communicated with the air pressure regulator and the third air path 231 are located on the first surface of the cartridge body 100, and the second air path is located on the second surface of the cartridge body 100, thus , the air pressure regulating device can be communicated with one end of the third air hole on the first surface of the cartridge body 100, so as to avoid the welding line of the second air path and the welding line of the third air path, thereby The airtightness of the connection between the air pressure regulating device and the cartridge is ensured.

The fifth air hole 205 is arranged on the third air channel 231 and the second air channel 921, the first air channel extends from the upper part of the first sample mixing chamber 1511, and the sixth air hole 206 is arranged on the second air channel 921 and the first air channel. on the road;

The second waterproof and breathable membrane 1520 is arranged on the second air groove 921, and the second waterproof and breathable membrane 1520 is used for the second air groove 921 to be waterproof and breathable, and can also discharge the air in the sample. In one embodiment, the second waterproof and breathable membrane 1520 is welded on the second air groove 921 . In another preferred embodiment, the second waterproof and breathable membrane 1520 is welded around the fifth air hole 205 and the sixth air hole 206 on the second air groove 921 . In this way, when the sample enters the sixth air hole 206, it is immediately blocked by the second waterproof and gas-permeable membrane 1520, thereby avoiding the risk of sample leakage. Optionally, a separate waterproof and gas-permeable membrane is directly welded on the sixth air hole 206, so that it can have better waterproof performance, so as to prevent the risk of sample leakage caused by liquid flowing out of the waterproof and gas-permeable membrane. Of course, welding the second waterproof and gas-permeable membrane around the fifth air hole 205 and the sixth air hole 206 is more effective, which can further avoid sample leakage.

Further, a second sealing film is provided on the outside of the second waterproof and gas-permeable film 1520 to seal the second air groove 921 , so that the gas communication between the fifth air hole 205 and the sixth air hole 206 can be realized. The first sample mixing chamber 1511 communicates with the third air hole 203 through the first air passage, the sixth air hole 206, the second air groove, the fifth air hole 205, the third air passage 231, the fourth air hole 204, and the second air passage in sequence. .

The seventh air hole 207 is arranged on the third air channel 231 and the third air channel 931, the second air channel extends from the upper part of the second sample mixing chamber 1521, and the eighth air hole 208 is arranged on the third air channel 931 and the second air channel . Since the second air passage and the third air groove are located on different surfaces of the cartridge body 100, to avoid the third air hole 203 from directly acting on the external sealing film when the external air pump is connected, causing the sealing film to Damaged under pressure, leading to the problem of sample leakage;

The third waterproof and gas-permeable membrane 1530 is arranged on the third air groove 931, and the third waterproof and gas-permeable membrane 1530 is used for the third air groove 931 to be waterproof and breathable, and can also discharge the air in the sample. In one embodiment, the third waterproof and breathable membrane 1530 is welded on the third air groove 931 . In another preferred embodiment, the third waterproof and breathable membrane 1530 is welded around the seventh air hole 207 and the eighth air hole 208 on the third air groove 931 . So that when the sample enters the eighth air hole, it is immediately blocked by the third waterproof and gas-permeable membrane 1530, thereby avoiding the risk of sample leakage. Optionally, the waterproof and gas-permeable membrane is directly welded on the eighth air hole, so that it can have better waterproof performance, so as to prevent the risk of sample leakage caused by liquid overflowing outside the waterproof and gas-permeable membrane. Of course, welding the third waterproof and gas-permeable membrane 1530 around the seventh air hole 207 and the eighth air hole 208 is more effective, which can further avoid sample leakage.

Further, a second sealing film is provided on the outside of the third waterproof and gas-permeable film 1530 to seal the third air groove 931 , so that gas communication between the seventh air hole 207 and the eighth air hole 208 can be realized. The second sample mixing chamber 1521 communicates with the third air hole 203 sequentially through the second air passage, the eighth air hole 208 , the third air groove, the seventh air hole 207 , the third air passage 231 , the fourth air hole 204 , and the second air passage.

The bottom of the sixth valve chamber 561 is provided with a sixth channel hole and three sixth protrusions, a sixth opening is opened on the side wall of the sixth valve chamber 561, and the three sixth protrusions are equally spaced along the first The six flow channel holes are arranged around, and the sample can flow into the sixth flow channel hole from the gap between the three sixth protrusions, and the bottom of the sixth silica gel pad is in contact with the sixth protrusion; when squeezing the sixth When the silica gel pad is removed, the sixth silica gel pad is deformed and blocks the third channel hole, so that the sample cannot flow into other chambers through the sixth channel hole.

The cartridge body 100 is provided with a sixth flow channel 256, a ninth air hole 209 and a seventh flow channel. One end of the sixth flow channel 256 communicates with the first PCR chamber 1611, and the other end of the sixth flow channel 256 communicates with the sixth flow channel 256. The passage holes are connected, one end of the seventh flow passage is connected with the sixth opening of the sixth valve chamber 561 , and the other end of the seventh flow passage is connected with the first air groove 911 through the ninth air hole 209 .

The bottom of the seventh valve cavity 571 is provided with a seventh channel hole and three seventh protrusions, a seventh opening is opened on the side wall of the seventh valve cavity 571, and the three seventh protrusions are equally spaced along the The seven flow channel holes are arranged around, and the sample can flow into the seventh flow channel hole from the gap between the three seventh protrusions, and the bottom of the seventh silica gel pad 572 is in contact with the seventh protrusion; When the seventh silica gel pad 572 is removed, the seventh silica gel pad 572 is deformed and blocks the third channel hole, so that the sample cannot flow into other chambers through the seventh channel hole.

The cartridge body 100 is provided with an eighth flow channel 258, a tenth air hole 2010 and a ninth flow channel 259, one end of the eighth flow channel 258 communicates with the second PCR cavity 1621, and the other end of the eighth flow channel 258 communicates with the seventh flow channel 258. The passage holes are connected, one end of the ninth flow passage 259 is connected with the seventh opening of the seventh valve cavity 571 , and the other end of the ninth flow passage 259 is connected with the first air groove 911 through the tenth air hole 2010 .

Wherein, the siphon portion 121, the first air passage 211, the third air passage 231, the seventh flow passage 257, and the ninth flow passage 259 are all grooves arranged on the first surface of the cartridge body 100;

The first flow path 251, the sixth flow path 256, the eighth flow path 258, the first air groove 911, the second air groove 921, the third air groove 931 and the second air path 221 are all arranged on the cartridge body 100 of the first Slots on both sides.

Parts of the second flow path 252, the third flow path 253, the fourth flow path 254, and the fifth flow path 255 are grooves arranged on the first face of the cartridge body 100, and other parts are grooves arranged on the cartridge body 100. The grooves on the second surface; for the same flow channel, the grooves on the first surface communicate with the grooves on the second surface through corresponding valve chambers, for example, for the fourth flow channel 254, The grooves on the first surface communicate with the grooves on the second surface through the fourth valve cavity 541 .

As shown in Figure 3, Figure 14 and 15, wherein, the first sealing film 1200 covers the first surface of the cartridge body 100, specifically, the first sealing film 1200 is welded to the first surface of the cartridge body 100, Used to seal the sample addition chamber 111, the sample cracking chamber 132, the first valve chamber, the sample filter chamber 141, the second valve chamber 521, the first sample mixing chamber 1511, the third valve chamber 531, the second sample mixing chamber 1521, Fourth valve cavity 541, fifth valve cavity 551, sixth valve cavity 561, seventh valve cavity 571 and other cavities, as well as sealed siphon part 121, first air passage 211, third air passage 231, seventh flow passage, The ninth flow channel and other grooves, thereby avoiding the leakage of the sample in the above-mentioned cavity or groove, causing the virus in the sample to infect the testing personnel or spread in the environment, causing the problem of detection accidents.

The sample adding chamber 111 and the first sealing film 1200 jointly form a closed space, that is, the sample adding chamber 110; the sample lysis chamber 132 and the first sealing film 1200 jointly form a closed space, that is, the sample lysis chamber 130; the sample filtering chamber 141 and the second A sealing film 1200 forms a closed space together, that is, the sample filter chamber 140; the first sample mixing chamber 1511 and the first sealing film 1200 jointly form a closed space, that is, the first sample mixing chamber 151; the second sample mixing chamber 1521 Together with the first sealing film 1200 , an airtight space is formed, that is, the second sample mixing chamber 152 .

As shown in FIG. 10 , the siphon part 121 and the first sealing film 1200 together form a closed space, that is, the siphon tube 120 .

The first air passage 211 and the first sealing film 1200 form the first air passage 210; the third air passage 231 and the first sealing film 1200 form the third air passage 230; the seventh flow passage and the first sealing film 1200 form the seventh channel 2570; the ninth channel 259 and the first sealing film 1200 form the ninth channel 2590; the first air passage and the first sealing film 1200 form the first air passage 1512; the second air passage and the first sealing film 1200 form the second air passage Road 1522.

Compared with other parts of the cartridge body 100, the upper surface of the stepped portion 170 is concave as a whole. In this embodiment, a third sealing film 1300 is separately provided to cover the surface of the stepped portion 170, specifically: the third sealing film 1300 Welded on the stepped portion 170 , the third sealing film 1300 is used to seal cavities such as the first PCR cavity 1611 and the second PCR cavity 1621 . The thickness of the third sealing film 1300 is 0.1 mm; the setting of the stepped portion 170 facilitates the processing and manufacturing of the cartridge, and also facilitates the opening of the first PCR cavity and the second PCR cavity with smaller thicknesses.

The first PCR cavity 1611 is sealed by the second sealing film 1400 and the third sealing film 1300, and jointly forms a closed space, that is, the first PCR chamber 161; the second PCR cavity 1621 is sealed by the second sealing film 1400 and the third sealing film 1300, and together form a closed space, that is, the second PCR chamber 162;

The second sealing film 1400 covers the second surface of the cartridge body 100 and is used to seal the first channel 251, the second channel 252, the third channel 253, the fourth channel 254, the fifth channel 255, the Grooves for the six flow passages 256, the eighth flow passage 258, the second air passage 221, etc.

The first channel 251 and the second sealing film 1400 form a first channel 2510; the second channel 252 and the second sealing film 1400 form a second channel 2520; the third channel 253 and the second sealing film 1400 form a third channel 2530, The fourth channel 254 and the second sealing film 1400 form the fourth channel 2540 , the fifth channel 255 and the second sealing film 1400 form the fifth channel 2550 , the sixth channel 256 and the second sealing film 1400 form the sixth channel 2560 , the eighth channel 258 and the sealing film 1400 form the eighth channel 2580, the second air channel 221 and the second sealing film 1400 form the second air channel 220;

The first waterproof and breathable membrane 1510 is arranged in the first air groove 911, and the first air chamber 910 is formed by sealing the second sealing membrane arranged on the outside; the second waterproof and breathable membrane 1520 is arranged in the second air groove 921, and passed The second sealing film arranged on the outside forms the second air chamber 920; the third waterproof and breathable film 1530 is arranged in the third air groove 931, and the third air chamber 930 is formed by the second sealing film arranged on the outside, and the second sealing The membrane 1400 covers the first waterproof and gas-permeable membrane 1510, the second waterproof and gas-permeable membrane 1520 and the third waterproof and gas-permeable membrane 1530, and is used to seal the first air chamber 910, the second air chamber 920 and the third air chamber 930, avoiding The samples in the first air chamber 910, the second air chamber 920 and the third air chamber 930 diffuse into the environment through the corresponding first waterproof and gas-permeable membrane 1510, the second waterproof and gas-permeable membrane 1520 and the third waterproof and gas-permeable membrane 1530, Infect the testing personnel, causing detection accidents; in addition, when the air circulates in the first air chamber 910, the second air chamber 920 or the third air chamber 930, the second sealing film can make the first air chamber 910, the second air chamber 920 and the outside of the third air chamber 930 are not connected to the atmosphere, so as to realize the adjustment of air pressure difference with other chambers.

Among them, the materials of the first waterproof and breathable membrane 1510, the second waterproof and breathable membrane 1520 and the third waterproof and breathable membrane 1530 can all be: nylon membrane, PTFE, polyethersulfone/poly or polyvinylidene fluoride, etc., which can play waterproof and breathable properties. Material.

The first air chamber 910 and the first airway are arranged on the opposite surface, and are connected through the second air hole 202, in order to avoid the direct connection between the air pump and the first airway, resulting in the process of inflating the latter after the air pump pumps air Especially in the process of pumping air, the sample enters the air pump through the first airway, and there is a greater risk of sample leakage. Through the structure of the cartridge in this embodiment, when the air pump is working, for example, when pumping air Even if the sample enters the first air channel, when it enters the first air chamber 910, it has been blocked by the first waterproof sealing film, so that the sample cannot enter the air pump through the first waterproof and gas-permeable film. The structure of the cartridge does not have the above-mentioned risk of sample leakage.

The thickness of the first sealing film 1200 and the thickness of the second sealing film 1400 were 0.1 mm.

In some embodiments, the first sealing film 1200 and the third sealing film 1300 can be an integral main sealing film 1201, and the thickness range of the first sealing film 1200, the second sealing film 1400 and the third sealing film 1300 is 0.1mm- 0.5mm;

The material of the first sealing film 1200, the second sealing film 1400 and the third sealing film 1300 can be polypropylene plastic sealing film or polyethylene plastic sealing film;

In this embodiment, it is preferably a polyethylene plastic sealing film (ie transparent PP film), and the selection is to match the main body of the cartridge. Since the main body of the cartridge in this embodiment is made of black PP material, if the laser welding process is to be used for sealing, the sealing film on the surface of this layer must also be the same kind of PP material, and the color is transparent, which can achieve the best effect of laser welding. Therefore, a transparent polyethylene plastic sealing film is used.

When using other materials for the main body of the cartridge, and in the case of non-laser welding process sealing, other forms of sealing film can also be used; each sealing film and the cartridge in this embodiment are sealed by laser welding process , and other processes can also be used for sealing, such as ultrasonic welding process, hot pressing process, etc.

As shown in Figures 11-13, the first valve cavity and the first silicone pad form the first needle valve 510, the second valve cavity 521 and the second silicone pad 522 form the second needle valve 520, the third valve cavity 531 and the third The silica gel pad 532 forms the third thimble valve 530, the fourth valve chamber 541 and the fourth silica gel pad 542 form the fourth thimble valve 540, the fifth valve chamber 551 and the fifth silica gel pad form the fifth thimble valve 550, and the sixth valve chamber 561 The sixth thimble valve 560 is formed with the sixth silica gel pad, and the seventh thimble valve 570 is formed by the seventh valve cavity 571 and the seventh silica gel pad 572 .

As shown in Figures 8 and 9, in the cartridge of this embodiment, the cartridge body sampling nozzle 300 is provided with a through hole 301, the through hole 301 communicates with the sample adding chamber 111, and the cover 400 is closed on the cartridge body sampling nozzle 300. above, it is used to block the through hole 301 on the cartridge sampling nozzle 300, so that the cartridge sampling nozzle 300 can be sealed to prevent foreign matter from entering the sample adding cavity 111 through the cartridge sampling nozzle 300, and to maintain the cartridge body The air pressure of each chamber in 100 also plays a role in preventing the liquid from flowing out of the cartridge, thereby avoiding the leakage of the sample from the cartridge, causing the virus infection in the sample to detect personnel or the amplification product to spread in the environment, resulting in detection The problem of accidents.

The cover 400 is provided with a step 401, and the step 401 and the through hole 301 are an interference fit. When the cover 400 covers the box body sampling nozzle 300, the cover 400 is matched with the through hole 301 of the box body sampling nozzle 300 through the step 401. relationship, so that the lid 400 can seal the box body sampling nozzle 300; when the inspector presses the lid 400 forcefully, after the lid 400 is closed on the box body sampling nozzle 300, it cannot be opened again, thereby preventing the sample from coming out of the box body. If the virus in the sample comes out of the sample injection nozzle 300, the virus in the sample will infect the testing personnel or spread in the environment, causing the problem of testing accidents.

Specifically: an elastic part 310 is arranged on the cartridge body sampling nozzle 300, and the end of the elastic part 310 is connected to the cover 400. When the cover 400 is not in use, it can stay on the cartridge body 100 through the connection of the elastic part 310. Avoid problems such as loss of the cover 400 , placing the cover 400 in other places after use, causing virus infection or contamination of the cover 400 leading to inaccurate test results and the like.

Wherein, the cover 400, the elastic part 310 and the cartridge body sample nozzle 300 are integrated, which can avoid the processing of the cartridge body 100, which affects the performance of the cartridge body 100, and is also convenient for processing and manufacturing, reducing the need for welding or other processes. A processing step for realizing the connection between the cover 400 , the elastic part 310 and the cartridge sampling nozzle 300 .

In some implementations, the cover and the box body sampling nozzle 300 are closed through a snap connection, specifically: the cover is provided with a first protrusion, the through hole 301 is provided with a first groove, and when the cover is closed When the box body sampling nozzle 300 is in the box, the first protrusion and the first groove cooperate with each other, so that the lid and the box body sampling nozzle 300 can be closed by snap connection; or, the lid is provided with a second groove , the through hole 301 is provided with a second protrusion, when the cover is closed in the box body sampling nozzle 300, the second protrusion and the second groove cooperate with each other, so as to realize the passage of the lid and the box body sampling nozzle 300 Snap connection for lid closure.

In some embodiments, the connection between the cover 400, the elastic part 310 and the sampling nozzle 300 of the box body can also be connected by means of welding or nested connection, wherein the connection between the cover 400 and the sampling nozzle 300 of the box body can be through The method of buckle is tightly closed; between the elastic part 310 and the cover 400 is rotatably connected, or when the elastic part 310 is rotatably connected to the box body sample nozzle 300, the gap between the cover 400 and the box body sample nozzle 300 Tight capping can be achieved by screwing, that is, when the elastic member and the lid 400 or the box body sampling nozzle 300 can achieve relative rotation, the lid and the box body sampling nozzle 300 can be screwed to achieve tightness. Covering, the way of screwing can be provided with external thread on the outer periphery of the cover, the through hole 301 of the box body sampling nozzle 300 is provided with an internal thread matching the external thread, between the cover and the box body sampling nozzle 300 Screw-in with internal and external threads.

The elastic part 310 and the cover 400 are rotatably connected, specifically: the cover 400 is provided with a slot in the axial direction, the elastic part 310 is provided with a sleeve, the sleeve is placed in the slot, and there is a gap between the sleeve and the slot Cooperate, so as to realize the rotatable connection between the elastic part 310 and the cover 400, or the first groove body is set on the elastic part 310, the first protruding ring is set on the outer periphery of the cover 400, and the first protruding ring is inserted into the second In a tank body, the first convex ring and the first tank body are in clearance fit, so that the rotatable connection between the elastic part 310 and the cover 400 is achieved; the implementation of the rotatable connection between the elastic part 310 and the cover 400 is not limited to the above-mentioned methods , can be set according to the actual situation, and any structure that can realize the rotatable connection between the elastic part 310 and the cover 400 can be used.

The elastic part 310 is rotatably connected to the box body sampling nozzle 300, specifically: a second groove is arranged on the outer periphery of the box body sampling nozzle 300, a corresponding second convex ring is arranged on the elastic part 310, and the second groove body It is connected with the second protruding ring, and the second groove body and the second protruding ring are in a clearance fit, so as to realize the rotatable connection between the elastic part 310 and the box body sampling nozzle 300; or the elastic part 310 is provided with a third groove body , the outer periphery of the box body sampling nozzle 300 is provided with a corresponding third protruding ring, the third protruding ring is connected to the third groove body, and the third groove body and the third protruding ring are in clearance fit, thereby realizing the elastic part 310 and the box body adding The sample nozzle 300 is rotatably connected; the elastic part 310 and the box body sample nozzle 300 are rotatably connected. The structure of rotating connection can be used.

In some implementations, the cover 400 can be an independent cover 400 separated from the sampling nozzle 300 of the cartridge body, and the cover 400 is directly covered on the sampling nozzle 300 of the cartridge body through a buckle connection or a screwing manner. The upper part of the through hole 301 of the box body sampling nozzle 300 is provided with a slope surface 302. The slope surface 302 is convenient for the lid 400 to be closed on the one hand, and on the other hand, it is convenient for the sample to be added, and can completely fall into the sample along the slope surface 302. Add to chamber 110.

In the cartridge of the present embodiment, the sample adding chamber 110 is used to store the sample added by the cartridge sampling nozzle 300, wherein the sample adding chamber 110 is composed of a first sealing film 1200 and a sample adding chamber, and the siphon tube 120 is composed of a siphon part 121 Composed with the first sealing film 1200, since the bottom of the sample adding chamber communicates with the siphon part 121, the sample added to the cartridge sample adding nozzle 300 will directly enter the sample adding chamber 110 and the siphon tube 120, because the siphon part The structure of 121 is a U-shaped structure, and the sample stays in the siphon tube 120 and the sample adding chamber 110. By closing the cover 400 on the box body sampling nozzle 300, it is sealed so that the sample in the sample adding chamber 110 cannot be removed from the box body. Flow out from the sampling nozzle 300, and after the cover 400 is closed on the sampling nozzle 300 of the box body, each chamber in the cartridge body 100 is completely sealed.

Wherein, the bottom of the sample adding chamber 111 is provided with an inclined surface, which facilitates the collection of samples at the bottom of the sample adding chamber 111, so that the sample enters the siphon 120, and the inclined surface is a smooth transition structure, which can also reduce the liquid residual volume in the sample adding chamber .

Under the combined action of the sample adding chamber 110 and the siphon tube 120, the sample can stay in the sample adding chamber 110 and the siphon tube 120 stably without other forces, even when the first channel hole is not closed, the sample will not enter to the sample lysis chamber 130 to flow into the sample filter chamber 140 . Since the sample needs to react in the sample lysis chamber 130 , the sample must stay in the sample lysis chamber 130 and cannot directly enter the sample filter chamber 140 for filtration. Under the combined effect of the sample adding chamber 110 and the siphon tube 120, the sample will not directly enter the sample cracking chamber 130 due to capillary action, let alone enter the sample filtering chamber 140, therefore, there is no need to close the first channel hole, Furthermore, there is no need to control the first flow channel hole, and there is no need to control the first flow channel hole. Further, the operation of adding samples to the cartridge without being installed on the detection equipment can be directly performed after the cartridge is taken out separately. In this way, the convenience of adding samples is improved, and the requirements for sample addition steps are reduced.

The siphon tube 120 is a reciprocating and folding structure. Specifically, the siphon tube 120 includes an inlet connection part 1221, a first straight tube part 1211, a first curved part 1212, a second straight tube part 1213, a second curved part 1214, and a third straight tube part 1215. One end of the inlet connection part 1221 communicates with the bottom of the sample adding chamber 110, one end of the first straight pipe part 1211 communicates with the other end of the inlet connection part 1221, and the other end of the first straight pipe part 1211 It is connected with one end of the first curved part 1212, one end of the first curved part 1212 is connected with one end of the second straight pipe part 1213, and one end of the second straight pipe part 1213 is connected with one end of the second curved part 1214. The other end of the second bent portion 1214 communicates with one end of the third straight pipe portion 1215 , and the other end of the third straight pipe portion 1215 communicates with the outlet connecting portion 1222 .

The sample addition chamber 110, the inlet connection part 1221 and the first straight pipe part 1211 together form a first U-shaped structure. When the cartridge is placed vertically, the sample enters the sample addition chamber 110, the inlet connection part 1221 and the first straight pipe. In the part 1211, due to the existence of the first U-shaped structure, the problem that the sample directly enters the sample lysis chamber 130 and the sample filter chamber 140 is effectively avoided, resulting in detection failure.

The second straight pipe part 1213, the second curved part 1214 and the third straight pipe part 1215 jointly form a second U-shaped structure, and the second U-shaped structure can further prevent the sample from directly entering the sample lysis chamber 130 and the sample filter chamber 140, The problem that caused the detection to fail. Even if some samples pass over the first U-shaped structure, they will stay on the second U-shaped structure and cannot enter the sample lysis chamber 130 .

The first straight pipe part 1211 is provided with a first choke valve 801. When the first choke valve 801 is used to open, the sample can flow through the first straight pipe part 1211. When the first choke valve 801 is closed, the sample And/or the air is blocked by the first choke valve 801, unable to circulate; the first choke valve 801 can further prevent the sample and/or air from entering the sample lysis chamber 130, and can directly control the first choke valve 801 is closed, so as to more effectively avoid the problem that the sample directly enters the sample lysing chamber 130 and the sample filtering chamber 140, resulting in detection failure. Moreover, through the first choke valve 801, the cartridge can be placed horizontally instead of vertically, and the sample will not flow into the sample lysis chamber 130, which can effectively prevent the sample from spreading everywhere in the cartridge channel. affect the detection accuracy.

The third straight pipe part 1215 is provided with a second choke valve. When the second choke valve is opened, the sample can flow through the second straight pipe part 1213. When the second choke valve is closed, the sample and/or air Blocked by the second choke valve, it cannot communicate; under the action of the first choke valve 801, the second choke valve can further prevent the sample and/or air from directly entering the sample lysis chamber 130, and can be used as The spare choke valve, when the first choke valve 801 cannot work effectively, the second choke valve can work effectively to prevent the sample from directly entering the sample lysis chamber 130 .

In some embodiments, a U-shaped structure is used to connect the sample addition chamber 110 and the sample lysis chamber 130. Specifically, one end of the inlet connection part 1221 communicates with the bottom of the sample addition chamber 110, and the other end of the inlet connection part 1221 communicates with the bottom of the sample addition chamber 110. One end of the connecting part 1210 is connected, the other end of the connecting part 1210 is connected with one end of the outlet connecting part 1222, and the other end of the outlet connecting part 1222 is connected with the upper part of the sample cracking chamber 130, and the connecting part 1210 can be directly the first connecting pipe .

The communicating portion 1210 can also be a first straight pipe portion 1211 , a first curved portion 1212 , a second straight pipe portion 1213 , a second curved portion 1214 and a third straight pipe portion 1215 , thereby forming a double U-shaped structure in this embodiment. Both the first choke valve 801 and the second choke valve are arranged on the communication part 1210, and the communication part 1210 does not necessarily only have the first choke valve 801 and the second choke valve, and there may be other choke valves, In order to prevent the flow of the sample and/or air from entering the sample lysis chamber 130 , this embodiment only needs to provide at least one flow blocking valve on the communication portion 1210 .

In some embodiments, while the above-mentioned U-shaped structure is adopted, a first choke valve 801 is provided on the first straight pipe part 1211, and the first choke valve 801 is used to further prevent samples and/or air from directly entering In the sample lysing chamber 130 and the sample filtering chamber 140 , the problem of detection failure is caused. At the same time, the cartridge can be placed horizontally instead of vertically, and the sample will not flow into the sample lysis chamber 130, which can effectively prevent the sample from spreading everywhere in the channel of the cartridge and affect the accuracy of detection.

In some embodiments, using the first U-shaped structure and the second U-shaped structure in the sample adding chamber 110 and the sample lysis chamber 130 can effectively prevent the sample from directly entering the sample lysis chamber 130 and the sample filtration chamber 140. Detection accuracy.

In some embodiments, the siphon tube 120 in this embodiment is not used, and a communication channel is directly set between the sample adding chamber 111 and the sample lysis chamber 130 for communication. The flow of/or air in the communication channel can also avoid the problem that the sample directly enters the sample lysis chamber 130 and the sample filter chamber 140 , resulting in detection failure. At the same time, the cartridge can be placed horizontally instead of vertically, and the sample will not flow into the sample cracking chamber 130, which can effectively prevent the sample from spreading everywhere in the channel of the cartridge and affect the accuracy of detection.

In the cartridge of this embodiment, the sample lysis chamber 130 is used as a reaction place for the sample to be cracked. The sample lysis chamber 130 is composed of a sample lysis chamber 132 and a first sealing film 1200. The sample lysis chamber 130 is heated by the heating module of the device. Heating is carried out, and after the temperature reaches the temperature required for sample cracking, the sample cracks.

Wherein, the sample cracking chamber 132 is provided with a baffle 131, and the baffle 131 is vertically arranged in the middle of the sample cracking chamber 132, facing the junction of the siphon part 121 and the sample cracking chamber 132; in a specific embodiment, the baffle 131 is a strip-shaped plate, and its cross section is a long rectangle; the baffle plate 131 of the present invention can have the following effects:

1) The baffle 131 can act as a buffer to prevent the sample from splashing due to too fast flow rate when the sample enters the sample lysis chamber 130;

2) The multiple valves set on the cartridge need external force to realize their functions. When the valves are not evenly distributed on the cartridge, the overall force on the cartridge will be uneven. In order to balance the force on the entire cartridge , to prevent the cartridge from being bent and deformed, the baffle 131 can provide a stress-bearing part for the cartridge, and applying a force to the cartridge from the position of the baffle 131 can play a role in balancing the force applied to the cartridge.

3) During the pyrolysis process, due to the heating of the sample lysing chamber 130, the cavity itself will expand due to heat, and when a force is applied to the position of the baffle 131, it can also prevent excessive expansion of the sealing film.

In the cartridge of this embodiment, before the sample enters the sample cracking chamber 130 from the sample adding chamber 110 through the siphon tube 120, the first needle valve 510 needs to be closed so that the sample in the sample cracking chamber 130 will not flow into the sample filtering chamber 140 In order to ensure that the sample can be fully lysed in the sample lysing chamber 130, if some samples pass through the first needle valve 510 before being lysed, enter the first channel 2510 and flow into the sample filter chamber 140, it will cause detection Inaccurate results or even test failures;

Therefore, before the sample enters the sample cracking chamber 130 through the siphon tube 120 from the sample adding chamber 110, the first sealing membrane 1200, which is located at the position of the first thimble valve 510, needs to be extruded by an external force, so that the extruding force passes through the first thimble valve 510. A sealing film 1200 is transferred to the first silica gel pad, and the first silica gel pad is squeezed and deformed to block the first channel hole, so that the sample cannot flow through the first channel 2510 into the sample filter chamber 140 through the first channel hole.

Among them, the structure of the first thimble valve 510, the second thimble valve 520, the third thimble valve 530, the fourth thimble valve 540, the fifth thimble valve 550, the sixth thimble valve 560 and the seventh thimble valve 570 are the same. , Therefore, the following description, illustration and effect on the structure of the thimble valve, for the first thimble valve 510, the second thimble valve 520, the third thimble valve 530, the fourth thimble valve 540, the fifth thimble valve 550, the first thimble valve Both the six thimble valve 560 and the seventh thimble valve 570 are applicable.

The thimble valve includes a valve cavity 511 , a rubber pad 512 and at least one protrusion 5111 . The side of the valve chamber 511 is provided with an opening 5113 , and the bottom is provided with a channel hole 5112 . The glue pad 512 is located above the channel hole 5112 . The raised part 5111 is provided on the surface of the rubber pad 512 facing the valve chamber 511, and/or, is provided on the surface of the valve chamber 511 at a position corresponding to the rubber pad 512, so that the undeformed rubber pad 512 and the flow channel hole 5112 A gap is formed between them for the sample to flow in, and the rubber pad 512 is used to deform and block the flow channel hole 5112 when being squeezed.

The working principle of the thimble valve structure proposed in this application is: due to the existence of the raised part 5111, when the rubber pad 512 is not subjected to external force (not deformed), see Figures 11 to 13, the gap between the rubber pad 512 and the flow channel hole 5112 There is a gap between them, the liquid sample can flow into the valve cavity 511 from the opening 5113, and then flow into the channel hole 5112, so as to flow out of the needle valve structure along the channel. When the rubber pad 512 is subjected to a predetermined pressure, it will be deformed, and the rubber pad 512 will be deformed to block the flow channel hole 5112, so that the liquid cannot flow into or flow out of the flow channel hole 5112, that is, it cannot pass through the thimble valve structure, so the thimble valve structure can be activated. to block fluid flow. Due to the elasticity of the rubber pad 512, when the external force is removed, the rubber pad 512 returns to its original shape, and returns to the state where there is a gap between the rubber pad 512 and the flow channel hole 5112, and the liquid can flow through the thimble valve structure again.

The thimble valve proposed by the present invention has a simple structure and reliable fluid control effect, and the structure of the thimble valve can be controlled only by a pressing action, thereby effectively simplifying the operation of supporting instruments.

When the rubber pad 512 is deformed under the extrusion of external force, the rubber pad 512 enters the middle of the three protrusions 5111, blocks the flow channel hole 5112, and prevents the sample from flowing through the flow channel hole 5112 through the first channel 2510 into the sample Inside the filter chamber 140. Due to the elasticity of the rubber pad 512, when the external force acts, the rubber pad 512 has a restoring elastic force. When the external force is removed, the rubber pad 512 returns to its original shape and is placed on the top of the three protrusions 5111. The sample can be read from three The middle of each protrusion 5111 passes through the channel hole 5112 and flows into the sample filter chamber 140 through the first channel 2510 . That is, the thimble valve includes: the thimble valve includes a valve cavity and a rubber pad, and the bottom of the valve cavity is provided with flow channel holes and several raised parts;

The rubber pad is placed above the raised part, and the raised part supports the rubber pad, so that a gap can be formed between the undeformed rubber pad and the flow channel hole for the sample to flow in. The rubber pad is used to deform and block the flow when it is squeezed. Road hole.

Wherein, the gaps between the three protrusions 5111 can also be the same, or the three protrusions 5111 are provided with pores inside, and the pores can allow samples to pass through; the three protrusions 5111 can be directly replaced by convex rings, and the convex rings Several holes are arranged inside the top, and the holes can allow samples to pass through, all of which can realize the function of the first thimble valve 510 in this embodiment.

In addition, there can be at least two protrusions 5111, or one. When there is one protrusion 5111, the protrusions 5111 can push up the rubber pad 512 when the rubber pad is not deformed, so that it is not deformed. There is a gap between the rubber pad and the flow channel hole 5112 for the sample to flow in. When the rubber pad 512 is squeezed, it deforms and blocks the flow channel hole, which can also realize the function of a thimble valve.

The height of the raised portion in this embodiment is 0.2mm, the height of the entire thimble valve is 1.2mm, and the raised portion accounts for about 16.7% of the height of the entire thimble valve, the thickness of the rubber pad in this embodiment is 1mm, and the hardness is 40A . For example, when the height of the raised portion increases, a rubber pad with softer hardness can be selected; and when the height of the raised portion decreases, a rubber pad with higher hardness can be selected. According to the fact that the raised portion accounts for 5%-30% of the height of the entire thimble valve, a silicone pad with a hardness in the range of 60A-30A can be selected. The above description of the structure of the thimble valve, in the first thimble valve 510 , the second thimble valve 520 , the third thimble valve 530 , the fourth thimble valve 540 , the fifth thimble valve 550 , the sixth thimble valve 560 and the seventh thimble valve 570 It can be applied to other thimble valves.

The thimble valve in this embodiment has good sealing performance, and can realize automatic reset, and will not react with the samples and reagents in the cartridge, causing test errors.

In some embodiments, the structure of the thimble valve can also be: a flow channel hole 5112 is provided at the bottom of the valve chamber 511, three protrusions 5111 are arranged on the rubber pad 512, and the three protrusions 5111 are arranged along the bottom surface of the rubber pad 512. The center of the rubber pad 512 is arranged around a certain gap, and the rubber pad 512 is arranged in the valve cavity 511, and the center of the central flow channel hole 5112 on the bottom surface of the rubber pad 512 corresponds to the center;

When the external force does not squeeze the rubber pad 512, that is, when the thimble valve is open, the sample flows through the gap between the three raised parts 5111, or directly flows through the bottom of the three raised parts 5111, and flows into the flow channel hole 5112, flows into the first channel 2510 through the channel hole 5112;

When the rubber pad 512 is squeezed by an external force, the rubber pad 512 is deformed, and the three first protrusions are against the bottom of the valve cavity, and the center of the rubber pad 512 blocks the flow channel hole 5112, so that the sample cannot flow from the flow channel hole 5112 to the first valve chamber. Inside a channel 2510 is the closed state of the first thimble valve.

When the external force is removed to squeeze the rubber pad 512, under the action of the three protrusions 5111 and the elasticity of the rubber pad 512 itself, the rubber pad 512 returns to its original shape, that is, the thimble valve returns to the open state for the next use.

In this embodiment, there can be one, two or four protrusions 5111, all of which can be realized. The protrusions 5111 mainly support the rubber pad 512 out of the gap for the sample to enter the flow channel hole 5112. When the rubber pad 512 is squeezed, the rubber pad 512 can seal the channel hole 5112 .

In some embodiments, the structure of the thimble valve can also be as follows: the bottom of the valve cavity 511 is provided with a flow channel hole 5112 and a convex ring, and several holes are provided on the periphery of the rubber pad 512, and the sample can pass through the peripheral holes on the rubber pad 512. The hole enters the flow channel hole 5112;

When the rubber pad 512 is squeezed by external force, the rubber pad 512 is deformed, and the center of the rubber pad 512 blocks the flow channel hole 5112, so that the sample cannot flow from the flow channel hole 5112 into the first channel 2510, which is the closed state of the thimble valve.

When removing the external force to squeeze the rubber pad 512, under the action of the elasticity of the collar and the rubber pad 512, the rubber pad 512 returns to its original shape, that is, the thimble valve returns to the open state for the next use.

In this method, a protrusion that matches the flow channel hole 5112 can be further provided at the center of the rubber pad 512. In the closed state of the thimble valve, the protrusion is inserted to block the flow channel hole 5112, which can more effectively The flow channel hole 5112 is blocked to prevent the sample from flowing into the flow channel hole 5112.

In some embodiments, instead of using the thimble valve in this embodiment to control, the method used can be to set a choke valve at the bottom of the sample lysis chamber 132, or to set the valve chamber 511 and the flow channel hole 5112 as well, and then A magnetic part is provided, and a sealing layer is set on the magnetic part; it is controlled by an external magnetic device, and when sealing is required, the sealing layer of the magnetic part is held against the flow channel hole 5112 by means of the external magnetic device in a manner of opposite sex repelling each other, Thus, the flow channel hole 5112 forms a seal, preventing the sample from entering the flow channel hole 5112 .

The functions to be realized by the thimble valve are: in the closed state, the flow channel hole 5112 is sealed to prevent the sample from flowing into the sample filter chamber 140 through the flow channel hole 5112 through the first channel 2510; 5112 is opened to allow the sample to flow into the sample filter chamber 140 through the flow channel hole 5112 through the first channel 2510 . It can be known from this embodiment and some other above-mentioned implementation manners that the thimble valve may also be other valves or switches capable of realizing the above-mentioned functions.

Wherein, the rubber pad 512 can also use natural rubber pads, EPDM rubber pads, and nitrile rubber pads, which have elasticity and inertness and are not easy to absorb nucleic acids. The rubber pad used in this embodiment is a silicone pad, which avoids the impact on the test due to the problem of the cartridge during the test.

In the cartridge of this embodiment, the sample filter chamber 141 is used to filter macromolecular impurities, mainly to filter out macromolecules exceeding 200 nm in the sample, so as to prevent macromolecular impurities from blocking the subsequent second channel 2520, third channel 2530, and second channel 2530. One or more of the four channels 2540, the fifth channel 2550, the sixth channel 2560, and the seventh channel 2570 result in reduced test efficiency, and if any of the flow channels are blocked, it may cause the test to fail.

The filter membrane can be made of polyester or nylon filter membrane, which can realize macromolecular filtration. The filter membrane is preferably a nylon filter membrane. The nylon filter membrane is cheap and can better achieve the filtering effect, effectively reducing the cost of the cartridge , especially in the batch production process; and it is found through PCR experiments that the use of polyester or nylon filter membranes has little influence on the test results.

In the cartridge in this embodiment, the first sample mixing chamber 1511 and the second sample mixing chamber 1521 can be cavities with the same shape and size, or cavities with different structural shapes and sizes. The first sample mixing chamber 151 is composed of a first sample mixing chamber 1511 and a first sealing film 1200 , and the second sample mixing chamber 152 is composed of a second sample mixing chamber 1521 and a first sealing film 1200 .

The first mixing beads 710 and the first freeze-drying beads 610 are located in the first sample mixing chamber 151, and the second mixing beads 720 and the second freeze-drying beads 620 are located in the second sample mixing chamber 152; the first freeze-drying beads 610 and the second freeze-dried beads 620 are nucleic acid amplification reaction reagents set according to the actual target to be measured. Generally, according to different targets, the first freeze-dried beads 610 and the second freeze-dried beads 620 are different freeze-dried beads, so that the cartridge can simultaneously measure a plurality of different targets for determination, which improves the detection function and detection efficiency of the cartridge .

The working mode and process of the first sample mixing chamber 151 and the second sample mixing chamber 152 are the same, but are determined for different targets. Therefore, taking the first sample mixing chamber 151 as an example, the first freeze-dried beads 610 are After the sample enters, it will be redissolved, and then the first mixing bead 710 is controlled to move up and down, so that the reconstituted freeze-dried bead powder and the sample are fully mixed and shaken, so as to realize the stable amplification of subsequent nucleic acids;

The first lyophilized beads 610 are placed in the first sample mixing chamber 151 instead of powdered reagents, because if the powdered reagents are used, when the sample enters the first sample mixing chamber 151, the powder The reconstitution effect of the lyophilized reagent is poor, and the production process is difficult to control, and the powder is easy to enter other rooms during transportation or placement, resulting in poor nucleic acid amplification and even test failure. If the reagent is freeze-dried in situ, the production process of the cartridge needs to put the entire cartridge into the freeze-drying chamber for freeze-drying, so that the production efficiency of the cartridge is relatively low, and the reconstitution effect of the in-situ freeze-dried reagent is to be Less effective in reconstitution than freeze-dried beads.

The main components of the first lyophilized beads 610 and the second lyophilized beads 620 not only include primers, probes, reverse transcriptase, hot start enzymes, dNTPs, enhancers, surfactants, salts, preservatives and other essential components of nucleic acid detection reagents . At the same time, substances such as excipients and protective agents must also be included. Excipients are usually inert substances, including sucrose, glucose, trehalose, melezitose, dextran and mannitol, etc. The amount of excipients should be appropriate, if the amount is too small, it cannot be molded, and if the amount is too large, it is difficult to redissolve. In the cartridge of this embodiment, since the first freeze-dried beads 610 and the first mixing beads 710 are stored in the first sample mixing chamber 151 at the same time, the hardness of the first freeze-dried beads 610 needs to have special requirements. If the amount of excipient is too small, it is easy to be broken by the impact of the first mixing beads 710 during transportation, which affects the performance of the subsequent reagent test. Too much excipient will increase the reconstitution time of the first freeze-dried beads 610 and the mixing time of the first mixing beads 710 , and the same is true for the second freeze-dried beads 620 . The weight of the excipient of the first freeze-dried beads 610 used in this embodiment accounts for the total weight of the first freeze-dried beads 610 and the excipient of the second freeze-dried beads 620 accounts for 20% of the total weight of the second freeze-dried beads 620. %-60%, which can effectively prevent the first freeze-dried beads 610 from being broken by the impact of the first mixing beads 710 during transportation, and will not increase the reconstitution time of the first freeze-dried beads 610 too much And the time for the first mixing beads 710 to mix.

Wherein, the design of the above-mentioned sample mixing chamber also adopts the smallest possible volume to ensure that there is space for the mixing beads and freeze-dried beads to be loaded at the same time. Its purpose is to:

1. The volume of the sample mixing chamber should not be too large to avoid the collision between the mixing beads and the freeze-dried beads during transportation. Because the volume of the sample mixing chamber is too large, the space for the two balls to move is also large, so that The mixing beads can accumulate greater kinetic energy to hit the lyophilized beads, making the lyophilized beads more susceptible to damage.

2. The volume of the sample mixing chamber should not be too small to ensure that the mixing beads have enough space for up and down movement, so that the sample and the reagent after reconstitution of the freeze-dried beads can be effectively mixed and moved.

The first freeze-dried bead 610 has a stable structure and can stay in the sample mixing chamber, and when the sample enters, because the first freeze-dried bead 610 has a porous structure inside, the contact between the sample and the first freeze-dried bead 610 The increased area enhances the reconstitution effect, and is easy to control, and due to the shape of the first freeze-dried beads 610, there will be no problem of powdery reagents entering other chambers. The second lyophilized beads 620 are the same as the first lyophilized beads 610 .

Under the action of an external magnet, the first mixing beads 710 can move in the first sample mixing chamber 151, and can actually move at various angles, so that the reconstituted first freeze-dried beads 610 and the sample can be mixed and shaken. In order to speed up the reconstitution of the first freeze-dried beads 610, so that the first freeze-dried beads 610 and the sample can be fully mixed, so as to ensure a high consistency of the detection results.

The first mixing beads 710 and the second mixing beads 720 can be round beads made of iron beads or alloys or magnetic beads, etc., which can move in the current corresponding sample mixing chamber under the action of magnetic force so that the first One lyophilized bead 610 or the second lyophilized bead 620 is smashed and reconstituted, and the first lyophilized bead 610 or the second lyophilized bead 620 is mixed and stirred, and its shape does not have to be spherical, and it can also be Structures such as polyhedrons, that is, the first mixing bead 710 can be the first mixing element, and the second mixing bead 720 can be the second mixing element.

In the first sample mixing chamber 151, when the sample enters the first sample mixing chamber 151, the first freeze-dried beads 610 are redissolved and mixed with the sample, and the first mixing beads 710 are controlled by an external magnet. Moving up and down, while knocking on the first freeze-dried beads 610, the reagents and samples reconstituted in the first freeze-dried beads 610 in the first sample mixing chamber 151 are mixed and stirred, and the first freeze-dried beads are fully and efficiently 610, the reconstituted reagent is fully mixed with the sample, so that after the subsequent sample mixed with the reagent enters the first PCR chamber 161, the PCR reaction can be performed, and the result with high consistency can be obtained, thereby effectively improving the follow-up process. The reliability of the detection of the sample (CV will be relatively small).

After the first freeze-dried beads 610 are reconstituted, the concentration of the reconstituted sample must be uniform to ensure that the reagent reaction system entering the first PCR chamber 161 is highly consistent each time. Put the first mixing beads 710 and the first freeze-drying beads 610 in the first sample mixing chamber 151; through the shaking and stirring of the first mixing beads 710, the first freeze-drying beads 610 are rapidly reconstituted, and at the same time The concentration of the sample after reconstitution of the first freeze-dried beads 610 can be realized to be uniform, thereby ensuring that the consistency of the reagent reaction system in the first PCR chamber 161 is kept within a relatively high range, and the upper and lower mixing beads 710 are used to Shaking and stirring can quickly mix the first freeze-dried beads 610 and the sample, thereby improving the reconstitution efficiency of the first freeze-dried beads 610 and improving the mixing efficiency of the first freeze-dried beads 610 and the sample.

The second sample mixing chamber 152 is the same as the first sample mixing chamber 151, and can quickly realize the rapid mixing of the sample and the PCR reaction reagent, so that the subsequent sample mixed with the reagent enters the first PCR chamber 161 for PCR. The reaction can obtain a result with high consistency; thus, the reliability of the PCR reaction in the second PCR chamber 162 is improved.

The first PCR chamber 161 is composed of the second sealing film 1400, the first PCR chamber 1611 and the third sealing film 1300, and the second PCR chamber 162 is composed of the second sealing film 1400, the second PCR chamber 1621 and the third sealing film 1300; The temperature of the first PCR chamber 161 and the second PCR chamber 162 is raised and lowered, and then the corresponding amplified nucleic acid is detected by the optical detection device, so as to obtain the detection result.

The first PCR chamber 161 corresponds to the first sample mixing chamber 151, which is determined by the reagents of the first freeze-dried beads 610 in the first sample mixing chamber 151, and the first freeze-dried beads 610 are specific for the desired amplification. Nucleic acid is set, the first PCR chamber 161 in this embodiment is used to detect COVID-19, and the corresponding first freeze-dried beads 610 are also enzymes and other corresponding reagents formulated according to COVID-19. The second PCR chamber 162 is used for determining FluA/FluB, and the corresponding second lyophilized beads 620 are also enzymes and other reagents formulated according to FluA/FluB. Wherein, both the first PCR chamber 161 and the second PCR chamber 162 can realize the detection of multiple targets in one PCR chamber; in order to avoid interference between the targets, it is best not to detect more than three targets in each PCR chamber. ; The first PCR chamber 161 and the second PCR chamber 162 in this embodiment realize the detection of multiple targets with high sensitivity.

When the liquid to be detected is full of the first PCR chamber 161 and the fourth channel 2540 is full of liquid, the fourth thimble valve 540 and the sixth thimble valve 560 are closed at this time. Since there is liquid in the fourth channel 2540, the fourth The thimble valve 540 will cause excessive pressure in the first PCR chamber 161 and the fourth channel 2540, and the above pressure will directly act on the second sealing film 1400 and the third sealing film 1300; Any one or both of the second sealing film 1400 and the third sealing film 1300 will cause liquid leakage, which will lead to detection failure; and if any one or both of the second sealing film 1400 and the third sealing film 1300 are supported If it is broken, it will cause samples with virus to enter the environment, and even cause the testing personnel to be infected;

In addition, since the first PCR chamber 161 needs to be tested by heating and cooling, when the first PCR chamber 161 and the fourth channel 2540 are full of samples, during the heating and cooling process, due to the principle of thermal expansion and contraction, the first PCR chamber 161 and the fourth channel 2540 are filled with samples. The pressure in the PCR chamber 161 increases, squeezing the third sealing film 1300, which may easily cause the third sealing film 1300 to be broken, resulting in liquid leakage, which in turn leads to detection failure, and causes samples with viruses to enter the environment , and even caused the problem of testing personnel being infected.

In view of the above-mentioned existing risks and problems, the cartridge body 100 is provided with a first damping chamber 1010, and the first damping chamber 1010 communicates with the upper part of the first PCR chamber 1611; On the second side, the first damping chamber 1010 and the second sealing film 1400 jointly form the first damping chamber 1011; when the sample fills the first PCR chamber 161 and the fourth channel 2540, the fourth needle valve 540 is closed, and the sample can enter into the first damping chamber 1011, thereby avoiding the problem of excessive pressure in the first PCR chamber 161 and the fourth passage 2540, and then effectively solving the problem of excessive pressure in the first PCR chamber 161 and the fourth passage 2540, resulting in Either one or both of the second sealing film 1400 and the third sealing film 1300 are broken, causing liquid leakage, which in turn leads to detection failure and virus-containing samples entering the environment, causing the problem of infection of test personnel;

And, the first damping chamber communicates with the first PCR chamber; when the temperature of the first PCR chamber filled with the liquid to be detected is raised and lowered, since the first PCR chamber is in a closed state under the control of the valve, the liquid cannot flow out from the valve. , when the temperature rises, it will cause excessive pressure in the first PCR chamber, and the first damping chamber connected with the first PCR chamber can allow the thermally expanded liquid to flow into it, thereby avoiding the problem of excessive pressure in the first PCR chamber, and then being able to It effectively solves the problem that the first PCR chamber is broken due to excessive pressure in the first PCR chamber, resulting in liquid leakage, which in turn leads to detection failure and virus-containing samples entering the environment, causing the detection personnel to be infected. When the temperature is lowered, the pressure in the first PCR chamber decreases, and the liquid in the first damping chamber flows into the first PCR chamber under the action of pressure at this time, so that good contact can be maintained between the heating and cooling device and the first PCR chamber , to avoid the problem of poor contact between the heating and cooling device and the first PCR chamber after cooling down due to the volume expansion of the first PCR chamber when the temperature is raised.

The first damping chamber 1011 is a channel structure. One end of the first damping chamber 1011 communicates with the first PCR chamber 161 and the other end is closed. When the first PCR chamber 161 is full, there is a space reserved in the first damping chamber 1011 . Specifically, the first damping chamber 1011 is a groove arranged on the cartridge, which is sealed by welding a sealing film on the surface to form a sealed channel structure, and an internal passage can also be dug out on the cartridge as the first damping chamber 1011. Wherein, the gap enables the liquid to continue to flow into the first damping chamber 1011 when the first PCR chamber 161 is heated, so as to relieve the pressure in the first PCR chamber 161 , thereby avoiding the problem of bursting the first PCR chamber 161 .

Wherein, the second end of the first damping chamber 1011 is higher than the first PCR chamber 161 .

Wherein, the first damping chamber 1011 is arranged parallel to the sixth channel 2560; the first damping chamber 1011 is located above the first PCR chamber 161, and the end of the first damping chamber 1011 is bent upward.

The setting of the first damping chamber 1011 also makes the selection of the valve more flexible and the selection range is wider, which solves the problem that the pressure of the first PCR chamber 161 will increase due to the liquid flow when the valve is closed. Taking the preferred thimble valve in this embodiment as an example, the thimble valve has a simple structure and a reliable fluid control effect. It can flexibly switch flow channels on the opposite surface of the cartridge, and the structure of the thimble valve can be controlled only by pressing, which can be effective. The advantage of simplifying the operation of supporting instruments; but when it is closed, it will increase the pressure of the first PCR chamber 161, and the setting of the first damping chamber 1011 can effectively solve this problem.

Similarly, the above-mentioned problems also exist in the second PCR chamber 162 and the fifth channel 2550. Therefore, the cartridge body 100 is provided with a second damping chamber 1020, and the second damping chamber realizes the same function as the above-mentioned first damping chamber. The chamber 1020 communicates with the upper part of the second PCR chamber 1621; the second damping chamber 1020 is arranged on the second surface of the cartridge body 100, and the second damping chamber 1020 and the second sealing film 1400 jointly form the second damping chamber 1021; when When the sample is full of the second PCR chamber 162 and the fifth channel 2550, after closing the fifth thimble valve 550 and the seventh thimble valve 570, the sample will enter the second damping chamber 1021, thereby avoiding the second PCR chamber 162 and the fifth passage. The problem of excessive pressure in the channel 2550 can effectively solve the problem that any one or both of the second sealing film 1400 and the third sealing film 1300 are caused by the excessive pressure in the second PCR chamber 162 and the fifth channel 2550. If it is broken, it will cause leakage, which will lead to the failure of the test and the entry of the sample with the virus into the environment, causing the problem of infection of the test personnel.

When the second PCR chamber 162 that is full of liquid to be detected is heated and lowered, because the second PCR chamber 162 is in a closed state under the control of the valve, the liquid cannot flow out from the valve, and the pressure in the second PCR chamber 162 will be caused when the temperature rises. is too large, and the second damping chamber communicated with the second PCR chamber 162 can allow the thermally expanded liquid to flow into it, thereby avoiding the problem of excessive pressure in the second PCR chamber, and then effectively solving the problem caused by the second PCR chamber The pressure inside 162 is too high, causing the second PCR chamber 162 to be burst, resulting in liquid leakage, which in turn leads to detection failure and virus-containing samples entering the environment, causing the detection personnel to be infected. When cooling down, the pressure in the second PCR chamber 162 becomes smaller, and at this moment, the liquid in the second damping chamber flows into the second PCR chamber 162 under the effect of pressure, so that the heating and cooling device and the second PCR chamber 162 can be maintained between the second PCR chamber 162. Good contact avoids the problem of poor contact between the heating and cooling device and the second PCR chamber 162 after cooling down due to volume expansion of the second PCR chamber when the temperature is raised.

Wherein, the first damping chamber 1011 communicates with the sixth channel, that is, the first damping chamber 1010 communicates with the sixth flow channel, and the first damping chamber 1011 communicates with the sixth channel.

The first damping chamber 1011 and the second damping chamber 1021 are channel structures; the first damping chamber 1011 is basically parallel to the sixth channel, and the second damping chamber 1021 is basically parallel to the eighth channel.

The sample adding chamber 111 is located at the upper right of the first surface of the cartridge body 100, the sample adding chamber 111, the siphon part 121 and the sample cracking chamber 132 are arranged in a row from right to left, and the sample filter chamber 141 is located at the lower right of the cartridge body 100 , and located directly below the siphon part 121;

The position of the first sample mixing chamber 1511 corresponds to that of the first PCR chamber 1611, and the position of the second sample mixing chamber 1521 corresponds to that of the second PCR chamber 1621; the first sample mixing chamber 1511 is located in the middle of the cartridge body 100, The first PCR chamber 1611 is located at the bottom left of the cartridge body 100 , the second sample mixing chamber 1521 is located above the first sample mixing chamber 1511 , and the second PCR chamber 1621 is located above the first PCR chamber 1611 . Through the above arrangement, the interference problem caused by air bubbles can be reduced during the flow of the sample in the cartridge body 100 .

The cartridge of the present embodiment, concrete working steps are as follows:

The first step: add the sample into the sample adding chamber 110 through the sampling nozzle 300 of the box body, and then press the lid 400, the lid 400 is closed on the sampling nozzle 300 of the box body, and the cartridge is sealed through the lid 400; ensure that the lid 400 After being pressed, it cannot be opened again.

Due to the effects of the siphon 120 between the sample adding chamber 110 and the sample lysing chamber 130, the first choke valve 801 and the second choke valve, the sample will stay in the siphon 120 and will not flow into the sample lysing chamber due to capillary action 130 in;

Step 2: Insert the cartridge into the testing device and fix it on the testing device. The first air pump of the detection equipment is connected with the first air hole 201, and the second air pump of the detection equipment is connected with the third air hole 203; wherein, the first air pump and the second air pump can be the same air pump, and work through different working ends.

The central rods that push the drive of the testing equipment squeeze the first thimble valve 510, the second thimble valve 520, the third thimble valve 530, the fourth thimble valve 540, the fifth thimble valve 550, the sixth thimble valve 560 and the first thimble valve respectively. Seven thimble valves 570, so that the first thimble valve 510, the second thimble valve 520, the third thimble valve 530, the fourth thimble valve 540, the fifth thimble valve 550, the sixth thimble valve 560 and the seventh thimble valve 570 are in a closed state , so that all channels in the cartridge are closed.

The third step: drive the first air pump, extract the air in the sample cracking chamber 130 through the first air hole 201, the first air hole 201 and the second air hole 202 communicate through the first air chamber 910, therefore, the sample staying in the siphon tube 120 will is sucked into the sample lysis chamber 130.

Step 4: start the heating module of the detection device, and heat the sample in the sample cracking chamber 130 to crack the sample;

Step 5: Release the central rod corresponding to the first thimble valve 510, the second thimble valve 520 and the third thimble valve 530 of the detection device; make the first thimble valve 510, the second thimble valve 520 and the third thimble valve 530 in an open state .

The second air pump of the driving detection device draws air into the cartridge through the third air hole 203; the third air hole 203 communicates with the fourth air hole 204 through the second air channel 220; after the third air hole 203 extracts air, it passes through the third air channel 230, the second air chamber 920 and the third air chamber 930, so that the air in the first sample mixing chamber 151 and the second sample mixing chamber 152 is sucked out, resulting in the first sample mixing chamber 151 and the second sample mixing chamber The air pressure at 152 changes, so that the lysed sample will flow into the sample filter chamber 140 through the first valve cavity, flow through the first channel 2510, and enter the first sample mixing chamber 151 and the second sample filter chamber 140 after filtering through the filter membrane. Two sample mixing chambers 1521.

Wherein, the sample entering the first sample mixing chamber 151 and the second sample mixing chamber 152 is filled with irregular order, may be filled one after the other, and may be filled at the same time; when the first sample mixing chamber 151 and the second sample mixing chamber After any chamber in the chamber 152 is full first, because the second waterproof gas-permeable membrane 1520 is arranged on the second gas chamber 920, and the third waterproof gas-permeable membrane 1530 is arranged on the third gas chamber 930, the first sample that is filled earlier is mixed. Under the action of the second waterproof gas-permeable membrane 1520 or the third waterproof gas-permeable membrane 1530, the chamber 151 or the second sample mixing chamber 152 cannot continue to enter the sample, so that the sample enters another sample mixing chamber until the two sample mixing chambers are blocked. At the same time, due to the existence of the second waterproof gas-permeable membrane 1520 and the third waterproof gas-permeable membrane 1530, the air on the upper layer of the sample is discharged, avoiding the formation of air bubbles in the first sample mixing chamber 151 and the second sample mixing chamber 152, affecting Subsequent detection of nucleic acid amplification reaction in the PCR cavity;

For example: when the sample fills the first sample mixing chamber 151, the sample continues to enter the sixth air hole 206 and after contacting the second waterproof and gas-permeable membrane 1520, the second waterproof and gas-permeable membrane 1520 will absorb and block the sixth air hole 206, The first sample mixing chamber 1511 cannot continue to flow into the sample. At this time, the sample will continue to fill the second sample mixing chamber 152. After filling the second sample mixing chamber 152, the sample will continue to enter the eighth air hole 208 and contact the third sample mixing chamber. Behind the waterproof and gas-permeable membrane 1530, the third waterproof and gas-permeable membrane 1530 will absorb and block the eighth air hole 208, so that the second sample mixing chamber 1521 cannot continue to flow into the sample, so that the sample is filled with the first sample mixing chamber 151 and mixed with the second sample. Room 152.

Step 6: After the sample flows into the first sample mixing chamber 151 and the second sample mixing chamber 152, it will be in contact with the first freeze-dried beads 610 and the second freeze-dried beads 620, and the first freeze-dried beads 610 and the second freeze-dried beads Freeze-dried beads 620 were quickly reconstituted;

Drive the central rod of the detection device corresponding to the first thimble valve 510, the second thimble valve 520, and the third thimble valve 530, and squeeze the first thimble valve 510, the second thimble valve 520, and the third thimble valve 530, so that the first thimble valve The valve 510, the second thimble valve 520, and the third thimble valve 530 are in a closed state; wherein, the second thimble valve 520 is closed for the purpose of the first mixing beads 710 in the first sample mixing chamber 151 for the sample and the first frozen During the mixing process of the reagents in the dry beads 610, they will not flow into the sample filter chamber 140 again, which avoids causing the first sample to The problem that the mixing effect of the sample in the mixing chamber 151 and the reagent of the first freeze-dried beads 610 is reduced; in the same way, the closing of the third thimble valve is also to avoid the flow of some samples in the second sample mixing chamber 152 back into the sample. In the filter chamber 140 , the mixing effect of the sample in the second sample mixing chamber 152 and the reagent of the second freeze-dried beads 620 is reduced.

Drive the electromagnet of the first mixing bead 710 and the second mixing bead 720, the electromagnet periodically generates magnetic force to attract the first mixing bead 710 to move upward and cancels the magnetic force to make the first mixing bead 710 go down due to its own gravity Movement, reciprocating control of the electromagnet makes the first mixing beads 710 move up and down in the first sample mixing chamber 151, and the first freeze-dried beads 610 are mixed with the sample evenly, so that the reconstituted beads in the first freeze-dried beads 610 Reagents and samples are completely mixed;

Similarly, the second mixing beads 720 also perform the same up-and-down movement in the second sample mixing chamber 152, and the second freeze-dried beads 620 are evenly mixed with the sample, so that the reconstituted reagent and sample in the second freeze-dried beads 620 Mix thoroughly.

Step 7: Drive the central rod of the detection equipment corresponding to the fourth thimble valve 540, the fifth thimble valve 550, the sixth thimble valve 560 and the seventh thimble valve 570, so that the fourth thimble valve 540, the fifth thimble valve 550, the The six thimble valves 560 and the seventh thimble valve 570 are in an open state;

The second air pump of the detection device pushes air into the cartridge through the third air hole 203, which will push the reacted sample in the first sample mixing chamber 151 into the first PCR chamber 161, and the second sample mixing chamber The samples in 152 are pushed into the second PCR chamber 162;

The same as the above-mentioned process of entering the first sample mixing chamber 151 and the second sample mixing chamber 152, the filling order of the first PCR chamber 161 and the second PCR chamber 162 has no rules, when the first PCR chamber 161 and the second PCR chamber 162 After any one of the PCR chambers is full, the other PCR chamber will be filled immediately, and it is also possible that the first PCR chamber 161 and the second PCR chamber 162 are full at the same time. Since the first air chamber 910 has a welded first waterproof and gas-permeable membrane 1510, when the sample enters the ninth air hole 209 through the seventh channel 2570, the first waterproof and gas-permeable membrane 1510 will absorb and block the ninth air hole 209, thereby making The first PCR chamber 161 cannot continue to enter the sample, and then the sample will continue to enter the second PCR chamber 162. When the sample enters the tenth air hole 2010 through the ninth channel 2590, the first waterproof and gas-permeable membrane 1510 will be absorbed and blocked. The tenth air hole 2010 is closed, so that the second PCR chamber 162 cannot continue to enter the sample, thereby realizing that the first PCR chamber 161 and the second PCR chamber 162 are all filled; at the same time, due to the first waterproof and gas-permeable membrane 1510, the air in the upper layer of the sample is discharged, avoiding the formation of air bubbles in the first PCR chamber 161 and the second PCR chamber 162, which affects the detection of the nucleic acid amplification reaction;

Step 8: Release the central rod of the detection device corresponding to the fourth thimble valve 540, the fifth thimble valve 550, the sixth thimble valve 560 and the seventh thimble valve 570, so that the fourth thimble valve 540, the fifth thimble valve 550, the The six thimble valves 560 and the seventh thimble valve 570 are in a closed state;

The first PCR chamber 161 and the second PCR chamber 162 are heated and cooled by the detection equipment, and the amplification result is detected by the optical detection module to obtain the detection result.

The cartridge of this embodiment has the following advantages:

(1) The cartridge of this embodiment realizes the integration process of sample lysis, nucleic acid amplification and detection, and realizes the automatic process from adding the sample to obtaining the detection result, which minimizes the process of human contact and reduces the The probability of infection of testers is more convenient and quick, and personnel without professional training can easily use it, which reduces the requirements for testers and has high reliability and accuracy of test results.

(2) In the cartridge of the present embodiment, the first sealing film 1200, the second sealing film 1400 and the third sealing film 1300 are fully enclosed and pollution-free to the cartridge, thereby ensuring the safety of the cartridge, and its The operation is simple, and there is no need to carry out testing in a special PCR laboratory, which reduces the requirements for the testing environment, and avoids the problem of virus leakage of samples caused by improper operation and infection of test personnel.

(3) In the cartridge of this embodiment, through the first mixing beads 710 moving up and down in the first sample mixing chamber 151 to accelerate the nucleic acid amplification reaction, the samples and nucleic acid detection reagents can be fully mixed, ensuring the accuracy of detection. consistency.

(4) The cartridge of this embodiment only adopts the thermal cracking method to process the sample and remove the complicated nucleic acid extraction steps, which can effectively shorten the total duration of nucleic acid detection, making the total duration of nucleic acid detection less than one hour, Even 45 minutes can complete the high-sensitivity detection of multiple targets in one sample at the same time.

(5) The existing method is to extract and amplify the nucleic acid through the sample. In this process, the sample is subjected to repeated washing steps, which can easily lead to the loss of nucleic acid, and each washing will cause about 10% to 20% loss. Nucleic acid is lost, and nucleic acid amplification testing is performed at the end, which may lead to inaccurate final test results; the cartridge in this example uses pyrolysis to process samples and is suitable for analysis of nasopharyngeal swabs and cervical/vaginal swabs For relatively clean sample types, there is no need to wash the nucleic acid amplification process, which avoids the loss of nucleic acid during the washing process.

(6) The cartridge of this embodiment can be sampled outside the testing equipment, which is convenient and fast, and is convenient for storage and transportation. The storage and transportation are all under normal temperature conditions, and no cold chain is required.

(7) In the cartridge of this embodiment, the lid 400 cannot be opened again after being closed, so as to avoid the virus leakage of the sample and cause the test personnel to be infected.

(8) In the cartridge of the present embodiment, reagents are only arranged in the first sample mixing chamber 151 and the second sample mixing chamber 152, and the reagents are the first freeze-dried beads 610 and the second freeze-dried beads of freeze-dried components. The beads 620, without liquid reagents, the first freeze-dried beads 610 and the second freeze-dried beads 620 can be stored at room temperature for a long time, so that the cartridge of this embodiment can be stored at room temperature.

(9) The cartridge of this embodiment controls the flow of the sample in the cartridge by driving the sample flow through the air pump, and uses the size design of each chamber to quantify, which simplifies the requirements for driving the air pump and enhances the consistency of detection sex.

(10) In the cartridge of the present embodiment, the thickness of the cartridge body 100 is greater than or equal to 1mm, wherein the thickness of the stepped portion 170 is less than 1mm, and the thickness of other regions is 2mm, which can be beneficial in the heat cycle process. Exchange speed, at the same time, if the cassette is placed vertically, if there is air overflow during the PCR sample reaction, due to the gravity factor, the air will go to the top of the PCR chamber, which will not affect the optical detection.

(11) In the cartridge of this embodiment, a filter membrane is provided in the sample filter chamber 140 for filtering large particles in the sample. When the sample flows through the sample filter chamber 140, if there are air bubbles in the sample, the air bubbles will stay above the sample filter chamber due to buoyancy and will not flow into the next chamber, while the remaining samples will flow into the next chamber, The large particles and air bubbles in the sample stay in the sample filter chamber 140 , thereby effectively preventing the large particles and air bubbles in the sample from clogging the flow channels and chambers, especially the first PCR chamber 161 and the second PCR chamber 162 .

(12) Compared with the existing kits, the cartridge of this embodiment has a simpler structure, and its detection effect is also better than that of the existing kits. The simplicity of the structure can effectively reduce the cost , easy to process and produce.

(13) In the cartridge of the present embodiment, the sample flows in the same direction from the sample adding chamber 110, the sample cracking chamber 130, the sample filtering chamber 140, the first sample mixing chamber 151 to the first PCR chamber 161 in sequence, This avoids the problem that the sample flows back and forth at the channel, resulting in increased detection errors.

In some embodiments, the sample addition chamber 110, the sample lysing chamber 130, the sample filtration chamber 140, the first sample mixing chamber 151, the second sample mixing chamber 152, the first PCR chamber 161, the second PCR chamber 162 and corresponding communication In addition to the structure in embodiment 1, the channels, air passages and air chambers can also be sealed by opening a cavity on the cartridge body 100 as in embodiment 1, such as the sample lysing chamber 132. The corresponding sample cracking chamber 132 sealing film is welded above it to seal to form the sample cracking chamber 130; the first sample mixing chamber 151 is sealed by welding the corresponding first sample mixing chamber 151 sealing film above it to form the first sample This mixing chamber 151 etc.;

Or, the sample adding chamber 110, the sample cracking chamber 130, the sample filtering chamber 140, the first sample mixing chamber 151, the second sample mixing chamber 152, the first PCR chamber 161 and the second PCR chamber 162 can also be composed of the sample adding chamber 111, the outer cover corresponding to the sample lysis chamber 132, the sample filter chamber 141, the first sample mixing chamber 1511, the second sample mixing chamber 1521, the first PCR chamber 1611 and the second PCR chamber 1621 and capable of sealing function or The combination of the cover and the body is formed;

Furthermore, the sample adding chamber 110, the sample cracking chamber 130, the sample filtering chamber 140, the first sample mixing chamber 151, the second sample mixing chamber 152, the first PCR chamber 161 and the second PCR chamber 162 can also be directly arranged on In the cartridge body 100, the first PCR chamber 161 and the second PCR chamber 162 offer windows for optical devices to detect;

Those skilled in the art can clearly understand the sample addition chamber 110, sample lysis chamber 130, sample filtration chamber 140, first sample mixing chamber 151, second sample mixing chamber 152, first PCR chamber 161, second PCR chamber 162, the sample adding chamber 110, the sample cracking chamber 130, the sample filtering chamber 140, the first sample mixing chamber 151, the second sample mixing chamber 152, the first PCR chamber 161, the second The PCR chamber 162 can be more easily observed through the first sealing film 1200 , the second sealing film 1400 and the third sealing film 1300 , which will not be repeated here.

The channel, airway and air chamber in Embodiment 1 can also be arranged inside the cartridge body 100, or through the above-mentioned sample adding chamber 110, sample cracking chamber 130, sample filtering chamber 140, first sample mixing chamber 151, The second sample mixing chamber 152 , the first PCR chamber 161 , and the second PCR chamber 162 are constructed in a manner that is clearly understood by those skilled in the art, and will not be repeated here.

Among them, the structure of the first thimble valve 510, the second thimble valve 520, the third thimble valve 530, the fourth thimble valve 540, the fifth thimble valve 550, the sixth thimble valve 560 and the seventh thimble valve 570 in Embodiment 1 There can be many kinds, and it can also be directly replaced by other valves that can also be opened and closed. The valve 520 only needs to be the fourth valve to realize opening and closing, the third thimble valve 530 only needs to be the third valve to realize opening and closing, and the fourth thimble valve 540 only needs to be the third valve to realize The fourth valve that opens and closes is sufficient, the fifth thimble valve 550 only needs to be the fifth valve that realizes opening and closing, the sixth thimble valve 560 only needs to be the sixth valve that realizes opening and closing, and the seventh valve. The thimble valve 570 only needs to be the seventh valve to realize opening and closing, which can be clearly understood by those skilled in the art, and will not be repeated here.

Among them, in Embodiment 1, the first air hole 201 is connected with the first air pump corresponding to the detection device, the third air hole 203 is connected with the second air pump of the detection device, and the sample is driven between the chambers by pumping or pushing air. and the flow in the channel; in other embodiments, only the third air hole 203 is used to drive the sample to flow between the chambers and the channel, and the first air pump can also be used to pump or push the first air hole 201 way to control the cartridge in other embodiments to achieve detection; the first air hole 201 and/or the third air hole 203 can be arranged in other positions, as long as it can push the sample from the sample lysis chamber 130 into the first PCR chamber 161 and the second PCR chamber 162, that is, the cartridge can be driven by a pneumatic module, and the pneumatic module includes the first air hole 201 connected to the air pump and/or the third air hole 203 and the ninth air hole 209 connected to the air pump , the tenth air hole, so that the pneumatic module is used to drive the sample to flow between the sample lysis chamber 130, the first sample mixing chamber 151 and the first PCR chamber 161; in addition, the pneumatic module may also include the above-mentioned first air hole 201 , the second air hole 202, the third air hole 203, the fourth air hole 204, the fifth air hole 205, the sixth air hole 206, the seventh air hole 207, the eighth air hole 208, the ninth air hole 209 and the tenth air hole, by using the pneumatic module It can play the role of driving the sample to flow between the sample lysis chamber 130, the first sample mixing chamber 151 and the first PCR chamber 161, and in the above-mentioned embodiment can play the role of driving the sample in the sample adding chamber 110, the siphon, the sample Lysis chamber 130, sample filter chamber 140, first sample mixing chamber, first PCR chamber and sample addition chamber 110, siphon, sample lysis chamber 130, sample filter chamber 140, second sample mixing chamber 152, second The communication between the PCR chambers 162 can be clearly understood by those skilled in the art from the above working steps, and will not be repeated here.

Example 2

A detection device according to another preferred embodiment of the present invention includes the above-mentioned cartridge, and supporting instruments used in conjunction with the cartridge;

The instrument mainly includes: a frame and set on the frame: the first air pump corresponding to the first air hole 201, the second air pump corresponding to the third air hole 203, the first central rod corresponding to the first thimble valve 510, The second central rod corresponding to the second thimble valve 520, the third central rod corresponding to the third thimble valve 530, the fourth central rod corresponding to the fourth thimble valve 540, and the fifth central rod corresponding to the fifth thimble valve 550 , the sixth central rod corresponding to the sixth thimble valve 560, the seventh central rod corresponding to the seventh thimble valve 570, and the corresponding position in the sample lysis chamber 130 are used to heat the sample in the sample lysis chamber 130 The cracking heating module is provided with a first magnetic attraction module at a position corresponding to the first sample mixing chamber 151, which is used to provide a magnetic attraction force, and control the attraction or release of the first mixing beads 710, corresponding to the second sample mixing chamber 152 There is a second magnetic attraction module at the position, which is used to provide magnetic attraction, control the attraction or release of the second mixing beads 720, and the first temperature control module and the first optical detection module are arranged at the corresponding position of the first PCR chamber 1611 And a second temperature control module and a second optical detection module are arranged at positions corresponding to the second PCR cavity 1621;

The first air pump and the second air pump can be realized by other air pressure regulating devices.

When the first thimble valve 510, the second thimble valve 520, the third thimble valve 530, the fourth thimble valve 540, the fifth thimble valve 550, the sixth thimble valve 560 and the seventh thimble valve 570 are replaced by other valves, the In the embodiment, the first thimble valve 510 is the first valve; the second thimble valve 520 is the second valve; the third thimble valve 530 is the third valve; the fourth thimble valve 540 is the fourth valve; the fifth thimble valve 550 is The fifth valve; the sixth thimble valve 560 is the sixth valve; the seventh thimble valve 570 is the seventh valve; the corresponding drive module can be preferably used, that is, the first drive module corresponding to the first valve, and the second valve corresponding to the second valve. The second driving module, the third driving module corresponding to the third valve, the fourth driving module corresponding to the fourth valve, the fifth driving module corresponding to the fifth valve, the sixth driving module corresponding to the sixth valve, and the sixth driving module corresponding to the seventh valve Seven drive modules.

There are installation slots on the frame, the installation slots are set vertically, and the cartridges are inserted vertically into the installation slots. When the cartridges are set vertically, it can effectively avoid the generation of air bubbles during the flow process between the sample channel and each chamber. , so as to avoid the interference of air bubbles, thereby effectively improving the accuracy of detection.

Wherein, the heating module can be an electric heater, which can heat the sample cracking chamber 130, so that the sample cracks;

The first magnetic attraction module and the second magnetic attraction module can be electromagnets, and the electromagnets drive the first mixing beads 710 or the second mixing beads 720 to move up and down, so that the samples in the first sample mixing chamber 151 and the first freeze-dried After the beads 610, the sample in the second sample mixing chamber 152 and the second freeze-dried beads 620 are fully shaken and mixed, the nucleic acid amplification reaction occurs in the first PCR chamber 161 and the second PCR chamber 162, and the electromagnet is powered on and off to drive The first mixing bead 710 or the second mixing bead 720 moves up and down, which is easy to operate and can effectively improve the mixing of the sample and the reagent of the freeze-dried beads in the first sample mixing chamber 151 and the second sample mixing chamber 152 degree, so that the results of the nucleic acid amplification reactions in the first sample PCR chamber 151 and the second PCR chamber 152 can maintain high consistency.

The working process of the detection equipment is as follows:

The first step: add the sample into the sample adding chamber 110 through the sampling nozzle 300 of the box body, and then press the lid 400, the lid 400 is closed on the sampling nozzle 300 of the box body, and the cartridge is sealed through the lid 400; ensure that the lid 400 After being pressed, it cannot be opened again.

Due to the effects of the siphon 120 between the sample adding chamber 110 and the sample lysing chamber 130, the first choke valve 801 and the second choke valve, the sample will stay in the siphon 120 and will not flow into the sample lysing chamber due to capillary action 130 in;

Step 2: Insert the cartridge vertically into the installation groove of the testing device; the first air pump communicates with the first air hole 201, and the second air pump communicates with the third air hole 203;

Push the first central rod to squeeze the first thimble valve 510, the second central rod to squeeze the second thimble valve 520, the third central rod to squeeze the third thimble valve 530, the fourth central rod to squeeze the fourth thimble valve 540, the The fifth center rod squeezes the fifth thimble valve 550, the sixth center rod squeezes the sixth thimble valve 560, and the seventh center rod squeezes the seventh thimble valve 570, so that the first thimble valve 510, the second thimble valve 520, the third The thimble valve 530 , the fourth thimble valve 540 , the fifth thimble valve 550 , the sixth thimble valve 560 and the seventh thimble valve 570 are in the closed state, so that all channels in the cartridge are in the closed state.

The third step: drive the first air pump, extract the air in the sample cracking chamber 130 through the first air hole 201, the first air hole 201 and the second air hole 202 communicate through the first air chamber 910, therefore, the sample staying in the siphon tube 120 will is sucked into the sample lysis chamber 130.

Step 4: start the heating module of the detection device, and heat the sample in the sample cracking chamber 130 to crack the sample;

Step 5: Release the first center rod, the second center rod and the third center rod of the detection device; make the first thimble valve 510 , the second thimble valve 520 and the third thimble valve 530 open.

On the one hand, under the action of gravity, the sample will flow through the first valve cavity, and then flow through the first channel 2510 into the sample filter chamber 140;

On the other hand, drive the second air pump to draw air in the cartridge through the third air hole 203; the third air hole 203 communicates with the fourth air hole 204 through the second air channel 220; 230, the second air chamber 920 and the third air chamber 930, so that the air in the first sample mixing chamber 151 and the second sample mixing chamber 152 is sucked out, causing the first sample mixing chamber 151 and the second sample mixing chamber The air pressure of the chamber 152 changes, so that the lysed sample will be sucked through the sample filter chamber 140 , filtered through the filter membrane, and then entered into the first sample mixing chamber 151 and the second sample mixing chamber 1521 .

Wherein, the sample enters into the first sample mixing chamber 151 and the second sample mixing chamber 152 to be filled with no rules, may be one after the other, and may be filled at the same time; when the first sample mixing chamber 151 and the second sample mixing chamber After any chamber in 152 is filled first, because the second waterproof gas-permeable membrane 1520 is arranged on the second gas chamber 920, the third waterproof gas-permeable membrane 1530 is arranged on the third gas chamber 930, makes the first sample mixing chamber full of first 151 or the second sample mixing chamber 152, under the action of the second waterproof and gas-permeable membrane 1520 or the third waterproof and gas-permeable membrane 1530, the sample cannot continue to enter, so that the sample enters another sample mixing chamber until the two sample mixing chambers are full Simultaneously, due to the existence of the second waterproof gas-permeable membrane 1520 and the third waterproof gas-permeable membrane 1530, the air on the upper layer of the sample is discharged, avoiding the formation of air bubbles in the first sample mixing chamber 151 and the second sample mixing chamber 152, affecting nucleic acid Detection of amplification reactions;

For example: when the sample fills the first sample mixing chamber 151, the sample continues to enter the sixth air hole 206 and after contacting the second waterproof and gas-permeable membrane 1520, the second waterproof and gas-permeable membrane 1520 will absorb and block the sixth air hole 206, The first sample mixing chamber 1511 cannot continue to flow into the sample. At this time, the sample will continue to fill the second sample mixing chamber 152. After filling the second sample mixing chamber 152, the sample will continue to enter the eighth air hole 208 and contact the third sample mixing chamber. Behind the waterproof and gas-permeable membrane 1530, the third waterproof and gas-permeable membrane 1530 will absorb and block the eighth air hole 208, so that the second sample mixing chamber 1521 cannot continue to flow into the sample, so that the sample is filled with the first sample mixing chamber 151 and mixed with the second sample. Room 152.

Step 6: After the sample flows into the first sample mixing chamber 151 and the second sample mixing chamber 152, it will be in contact with the first freeze-dried beads 610 and the second freeze-dried beads 620, and the first freeze-dried beads 610 and the second freeze-dried beads Freeze-dried beads 620 were quickly reconstituted;

Drive the first central rod to squeeze the first thimble valve 510, the second central rod to squeeze the second thimble valve 520, and the third central rod to squeeze the third thimble valve 530, so that the first thimble valve 510, the second thimble valve 520 , the third thimble valve 530 is in a closed state;

Drive the first magnetic attraction module and the second magnetic attraction module, the first magnetic attraction module and the second magnetic attraction module are periodically powered on and off, the magnetic force is generated by energizing to absorb the first mixing beads 710 to move upward, and the magnetic force is canceled by turning off the power so that The first mixing bead 710 moves downward due to its own gravity, and the electromagnet is controlled reciprocally so that the first mixing bead 710 moves up and down in the first sample mixing chamber 151, and the first freeze-dried bead 610 is mixed with the sample evenly, thereby allowing the reconstituted reagent in the first lyophilized bead 610 to be thoroughly mixed with the sample;

Similarly, the second mixing beads 720 also perform the same up-and-down movement in the second sample mixing chamber 152, and the second freeze-dried beads 620 are evenly mixed with the sample, so that the reconstituted reagent and sample in the second freeze-dried beads 620 Mix completely.

Step 7: Release the fourth central rod, the fifth central rod, the sixth central rod and the seventh central rod, so that the fourth thimble valve 540, the fifth thimble valve 550, the sixth thimble valve 560 and the seventh thimble valve 570 are in open state;

Push air into the cartridge through the third air hole 203 by the second air pump, the reacted sample in the first sample mixing chamber 151 will be pushed into the first PCR chamber 161, and the reacted sample in the second sample mixing chamber 1521 The sample is pushed into the second PCR chamber 162;

The same as the above-mentioned process of entering the first sample mixing chamber 151 and the second sample mixing chamber 152, the filling order of the first PCR chamber 161 and the second PCR chamber 162 has no rules, when the first PCR chamber 161 and the second PCR chamber 162 After any one of the PCR chambers is full, the other PCR chamber will be filled immediately, and it is also possible that the first PCR chamber 161 and the second PCR chamber 162 are full at the same time. Since the first air chamber 910 has a welded first waterproof and gas-permeable membrane 1510, when the sample enters the ninth air hole 209 through the seventh channel 2570, the first waterproof and gas-permeable membrane 1510 will absorb and block the ninth air hole 209, thereby making The first PCR chamber 161 cannot continue to enter the sample, and then the sample will continue to enter the second PCR chamber 162. When the sample enters the tenth air hole 2010 through the ninth channel 2590, the first waterproof and gas-permeable membrane 1510 will be absorbed and blocked. The tenth air hole 2010 is closed, so that the second PCR chamber 162 cannot continue to enter the sample, thereby realizing that the first PCR chamber 161 and the second PCR chamber 162 are all filled; at the same time, due to the first waterproof and gas-permeable membrane 1510, the air in the upper layer of the sample is discharged, avoiding the formation of air bubbles in the first PCR chamber 161 and the second PCR chamber 162, which affects the detection of the nucleic acid amplification reaction;

Step 8: Drive the fourth center rod, the fifth center rod, the sixth top center rod and the seventh center rod, so that the fourth thimble valve 540, the fifth thimble valve 550, the sixth thimble valve 560 and the seventh thimble valve 570 is closed;

The first temperature control module performs a heating and cooling cycle on the first PCR chamber 161, and the second temperature control module performs a heating and cooling cycle on the second PCR chamber 162 respectively, and the amplification result is detected by the first optical detection module. In a PCR chamber 161, the detection result is obtained; in the second PCR chamber 162, the detection result is obtained by detecting the amplification result through the second optical detection module.

Wherein, the detection equipment also includes a controller and a display, the controller is a host, and the controller is electrically connected to the first air pump, the second air pump, the first central rod, the second central rod, the third central rod, the fourth central rod, the fifth Central pole, sixth central pole, seventh central pole, heating module, first magnetic attraction module, second magnetic attraction module, first temperature control module, second temperature control module, first optical detection module and second optical detection module;

The controller is used to control the first air pump, the second air pump, the first central pole, the second central pole, the third central pole, the fourth central pole, the fifth central pole, the sixth central pole, the seventh central pole, the heating module, The first magnetic attraction module, the second magnetic attraction module, the first temperature control module, the second temperature control module, the first optical detection module and the second optical detection module complete the above working process.

The display is electrically connected to the controller for displaying that the controller controls the first air pump, the second air pump, the first central rod, the second central rod, the third central rod, the fourth central rod, the fifth central rod, and the sixth central rod , the seventh central rod, the heating module, the first magnetic attraction module, the second magnetic attraction module, the first temperature control module, the second temperature control module, the first optical detection module and the second optical detection module complete the above steps, And when a fault occurs in any step, the problem of this step will be displayed and a prompt message will be issued.

The detection equipment proposed by the present invention can realize the automatic processing of nucleic acid amplification and detection, the detection result is more accurate, and only a small number of manual operation steps are required, and the operation is simple, safe and convenient. Using the device for detection, the detection time is less than one hour, and the manual operation does not exceed 2 minutes, and it can be used to detect pathogen genome targets of various human clinical samples, and has wide applicability. The storage and transportation of the device of this application are all under normal temperature conditions, no cold chain is needed, and the economy is high. The cartridge of the device of the present invention is completely closed during the detection process, and its detection result is completely consistent with the conventional method, and can be operated without professional training, which is safe and convenient.

Wherein, the first air pump and the second air pump in Embodiments 1 and 2 can also be realized by using one air pump through two output ends;

In addition, the effect of the cartridge and the description of related components can be obtained directly from Embodiment 1 and other embodiments, and those skilled in the art can clearly understand it, and will not repeat it here.

Based on Embodiment 1 and Embodiment 2, a gas communication structure is also provided in the embodiment of the present application, and the gas communication structure includes:

Second air tank 921;

The fifth air hole 205, the fifth air hole 205 communicates with the second air groove 921, and is used to connect the air pressure regulating device;

The sixth air hole 206, the sixth air hole 206 is connected to the second air groove 921, and the sixth air hole 206 is provided with a waterproof and breathable membrane I, which is used to communicate with the chamber that allows liquid to flow in;

A second sealing film 1400 is disposed on the second air groove 921 to form a second air chamber 920;

Wherein, the fifth air hole 205 and the sixth air hole 206 realize gas communication in the second air chamber 920 .

The second sealing film 1400 can seal the second gas groove 921, so that the gas in the second gas groove 921 does not communicate with the outside atmosphere, so that the gas communication can be formed between the fifth gas hole 205 and the sixth gas hole 206, through The air pressure regulating device draws air or inflates air, so that the liquid in the cartridge can communicate between the chambers.

Wherein, the chamber communicated with the sixth air hole 206 is the first sample mixing chamber 151 in this embodiment, and it can also be the sample cracking chamber 130, the second sample mixing chamber 152, the second sample mixing chamber 151 in other embodiments. A PCR chamber 161 and a second PCR chamber 162;

The waterproof breathable film on the sixth air hole 206 can effectively prevent liquid from overflowing into the second air groove 921, and at the same time, the second sealing film 1400 can further effectively prevent the liquid from overflowing outside the cartridge, while the second sealing film The setting of 1400 can realize gas communication between the fifth air hole 205 and the sixth air hole 206 .

Further, the waterproof and gas-permeable membrane is welded on the second air groove 921 around the sixth air hole 206 . In order to facilitate the installation of the waterproof and gas-permeable membrane and effectively prevent liquid from overflowing out of the second air groove 921 , the waterproof and gas-permeable membrane is welded on the second air groove 921 by laser spot welding and located around the sixth air hole 206 .

Further, the fifth air hole 205 is provided with a second waterproof and breathable membrane. The second waterproof and breathable membrane is to further prevent liquid from entering the fifth air hole 205 from the sixth air hole 206 after penetrating the first waterproof and breathable membrane.

Further, the first waterproof and breathable membrane and the second waterproof and breathable membrane are the same second waterproof and breathable membrane 1520 . The second waterproof and breathable membrane is attached on the second air groove 921, and is located above the fifth air hole 205 and the sixth air hole 206, and is welded on the surface of the fifth air hole 205 and the sixth air hole 206 by laser spot welding, which is convenient for processing manufacturing, and can effectively ensure that the fifth air hole 205 and the sixth air hole 206 will not have liquid overflow.

Further, the second waterproof and breathable membrane covers the second air groove 921 and is welded to the second air groove 921 around the fifth air hole 205 and the sixth air hole 206 .

A kind of cartridge is also provided in the embodiment of the present application, and the cartridge comprises:

The gas communication structure as above;

Cartridge body 100;

Wherein, the second air groove 921 , the fifth air hole 205 and the sixth air hole 206 are all set on the cartridge body 100 , and the second sealing film 1400 is installed on the surface of the cartridge body 100 .

The cartridge body 100 can provide support for the second air groove 921, the fifth air hole 205 and the sixth air hole 206, the second air groove 921 is a groove body opened on the cartridge body 100, the fifth air hole 205 and the sixth air hole 206 They are all holes on the cartridge body 100 , which have been described in detail in Embodiment 1 and will not be repeated here.

Further, it also includes:

The third air hole 203, the second air channel 220 and the third air channel 230 provided on the cartridge body 100;

Wherein, one end of the third air hole 203 communicates with the air pressure regulator; one end of the second air channel 220 communicates with the other end of the third air hole 203; one end of the third air channel 230 communicates with the other end of the second air channel 220, The other end of the third air channel 230 communicates with the fifth air hole 205;

One end of the third air hole 203 communicating with the air pressure regulator is located on the same surface of the cartridge body 100 as the third air channel 230 , and the second air channel 220 and the third air channel 230 are located on different surfaces of the cartridge body 100 .

Specifically, the cartridge body 100 is provided with a second air passage 221 and a third air passage 231, and the second air passage 221 and the third air passage 231 are respectively located on opposite surfaces, that is, the second air passage 221 is located on the cartridge body 100. On the second surface of the cartridge body 100 , the third air passage 231 is located on the first surface of the cartridge body 100 .

The second air passage 221 is welded with the second sealing film 1400 on its surface for sealing to form the second air passage 220 , and the third air passage 231 forms the third air passage 230 by welding the main sealing film or the first sealing film on its surface.

The second air path 221 and the third air path 231 are communicated through the fourth air hole. Since the second air path 221 and the third air path 231 are not located on the same side of the cartridge body 100, the second air path is connected through the fourth air hole. The passage 221 and the third air passage 231, so that the air can flow between the fourth air hole and the second air passage 221 and the third air passage 231.

The air pressure regulating device is an air pump or the like, which pumps or inflates the second air channel 220 to change the pressure in the second air chamber 920 .

Further, the second air groove 921, the fifth air hole 205 and the sixth air hole 206 are all arranged on the cartridge body 100, and the sealing film on the second air channel 220 and the sealing film of the second air chamber 920 are all in the same piece The second sealing film 1400, wherein the second sealing film 1400 is bonded on the second surface of the cartridge body 100, specifically welded and sealed on the peripheral side of the second air passage 221 and the peripheral side of the second air groove 921 , so as to form the second air channel 220 and the second air chamber 920 that are not in contact with the outside atmosphere, thereby avoiding the problem of leakage of the liquid with the sample.

The second air passage 220 and the third air passage 230 are arranged on different surfaces of the cartridge body 100, so that the second air passage 220 and the third air passage 230 are provided, and at the same time, there is enough position to set the air pressure adjustment device, Such as air pumps, etc., in addition, the third air passage 230 will not be affected by the installation of the air pressure adjustment device. If the air pressure adjustment device is directly installed on the third air passage 230, when inflating or pumping air, it will directly act On the third airway 230, it is easy to cause the third airway 230 to be crushed under the direct action of the change of air pressure, causing the cartridge to be damaged and unusable. Transition through the second airway 220 can effectively avoid Or when pumping air, it directly acts on the third air passage 230, which will easily cause the third air passage 230 to be crushed under the direct action of the air pressure change, causing the cartridge to be damaged and unusable.

Further, the second air channel 220 and the second air groove 921 are located on the same surface of the cartridge body 100 . The air pressure regulating device is not located on the same surface of the cartridge body 100 as the second air groove 921, and enough space is reserved for the installation of the air pressure regulating device, so that the installation of the air pressure regulating device can be facilitated, and the installation of the air pressure regulating device can be avoided. , affecting the second air groove 921.

Further, the cartridge also includes a first sample mixing chamber 151 disposed on the cartridge body 100;

The first sample mixing chamber 151 communicates with the sixth air hole 206; the first sample mixing chamber 151 is provided with a first freeze-dried bead 610 and a first mixing bead 710, and the first mixing bead 710 moves the sample and the first mixing bead 710 A freeze-dried beads 610 for mixing;

The first sample mixing chamber 151 is also provided with a liquid inlet and a liquid outlet, the liquid inlet is connected to the second channel 2520 for the sample to enter the first sample mixing chamber 151, and the liquid outlet is connected to the fourth channel for the liquid output 2540.

Specifically, the first freeze-dried beads include excipients, and the weight of the excipients accounts for 20%-60% of the total weight of the first freeze-dried beads.

Under the action of the air pressure regulating device, the gas communication structure can not only guide the flow of the liquid in the connected chamber, but also prevent the liquid from continuing to flow into the chamber after the chamber is filled, so as to process the liquid in the chamber , so as to meet the application of specific functional scenarios.

The above is only a preferred embodiment of the present invention, it should be pointed out that for those of ordinary skill in the art, without departing from the technical principles of the present invention, some improvements and replacements can also be made, and these improvements and replacements are also possible. It should be regarded as the protection scope of the present invention.

Claims (10)

1. A gas interworking structure, said gas interworking structure comprising:

a second gas tank;

a fifth air hole communicated with the second air groove and used for connecting the air pressure adjusting device,

the sixth air hole is communicated with the second air groove, and a first waterproof breathable film is arranged on the sixth air hole and is used for communicating a chamber allowing liquid to flow in;

a second sealing film is arranged on the second air groove to form a second air chamber;

and the fifth air hole and the sixth air hole are communicated with each other in the second air chamber.

2. The gas intercommunication structure of claim 1, wherein the waterproof gas permeable membrane is welded to the second gas tank around the sixth gas hole.

3. The gas communication structure according to claim 2, wherein a second waterproof and breathable membrane is disposed over the fifth vent.

4. The gas communication structure of claim 3, wherein the first waterproof gas permeable membrane and the second waterproof gas permeable membrane are a same second waterproof gas permeable membrane.

5. The gas communication structure according to claim 4, wherein said second waterproof and breathable membrane covers said second gas groove and is welded to said second gas groove around said fifth gas hole and said sixth gas hole.

6. A cartridge, characterized in that the cartridge comprises:

the gas communication structure of any of claims 1-5;

a card box body;

the second air groove, the fifth air hole and the sixth air hole are formed in the card box body, and the second sealing film is arranged on the surface of the card box body.

7. The cartridge of claim 6, further comprising:

the third air hole, the second air passage and the third air passage are arranged on the card box body;

one end of the third air hole is communicated with the air pressure adjusting device; one end of the second air passage is communicated with the other end of the third air hole; one end of the third air passage is communicated with the other end of the second air passage, and the other end of the third air passage is communicated with the fifth air hole;

the third air hole and one end communicated with the air pressure adjusting device are located on the same face of the card box body through the third air passage, and the second air passage and the third air passage are located on different faces of the card box body.

8. The cartridge of claim 7, wherein the second air channel and the second air slot are located on the same face of the cartridge body.

9. The cartridge of claim 7, further comprising a first sample mixing chamber disposed on the cartridge body;

the first sample mixing chamber is communicated with the sixth air hole; a first freeze-drying bead and a first mixing bead are arranged in the first sample mixing chamber, and the first mixing bead is used for mixing a sample and the first freeze-drying bead uniformly through movement;

the first sample mixing chamber is also provided with a liquid inlet and a liquid outlet, the liquid inlet is communicated with a second channel for the sample to enter the first sample mixing chamber, and the liquid outlet is communicated with a fourth channel for the liquid to be output.

10. The cartridge of claim 9, wherein the first lyophilized bead comprises an excipient in an amount of 20% -60% by weight of the total weight of the first lyophilized bead.

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