CN115350321B

CN115350321B - Hydrogel dressing and preparation method thereof

Info

Publication number: CN115350321B
Application number: CN202211164608.9A
Authority: CN
Inventors: 牛长梅; 杨熙; 王丽阳
Original assignee: Suzhou Nuopu Regenerative Medicine Co ltd
Current assignee: Suzhou Nuopu Regenerative Medicine Co ltd
Priority date: 2022-09-23
Filing date: 2022-09-23
Publication date: 2023-10-20
Anticipated expiration: 2042-09-23
Also published as: CN115350321A

Abstract

The application relates to the field of biological medicine, and discloses a hydrogel dressing and a preparation method thereof, wherein the hydrogel dressing comprises the following components in percentage by mass: sodium alginate 0.5-5%, collagen 0.1-10%, glycerol 1-20%, carbomer 0.3-5%, polyvinyl alcohol 0.5-10%, antibacterial component 0.01-2%, and water 75-95%. The preparation method comprises the steps of uniformly mixing the components. The hydrogel dressing provided by the application has excellent film forming property and moisture retention property, and has remarkable capability of promoting wound healing rate.

Description

Hydrogel dressing and preparation method thereof

Technical Field

The application relates to the technical field of biological medicine, in particular to a hydrogel dressing and a preparation method thereof.

Background

The traditional dressing has the problems of dry wound, easy infection, easy adhesion, slow healing speed and the like in the aspect of wound care.

The hydrogel dressing has good water absorbability, can be repeatedly hydrated when contacting with a wound surface, and has the dual functions of providing water for the wound surface and absorbing seepage. The hydrogel dressing can promote better healing of wounds, absorb wound exudates, is not adhered to the wounds, reduces pain of patients, provides moist microenvironment for the wounds, and has good air permeability and biocompatibility.

Although there are many hydrogel dressings, the hydrogel dressings have poor application performance, but the field has been developed to be mature, and the aim of breaking through the hydrogel dressing with better performance is a problem to be solved at present.

In view of this, the present application has been made.

Disclosure of Invention

The application aims to provide a hydrogel dressing and a preparation method thereof.

The application is realized in the following way:

in a first aspect, the present application provides a hydrogel dressing, which comprises the following components in percentage by mass:

sodium alginate 0.5-5%, collagen 0.1-10%, glycerol 1-20%, carbomer 0.3-5%, polyvinyl alcohol 0.5-10%, antibacterial component 0.01-2% and deionized water 75-95%;

the antibacterial component is at least one selected from the group consisting of parabens, phenoxyethanol, p-hydroxyacetophenone and polyhexamethylene guanidine.

In an alternative embodiment, the hydrogel dressing composition comprises, in mass percent: 1 to 3 percent of sodium alginate, 0.5 to 5 percent of collagen, 3 to 10 percent of glycerol, 1 to 3 percent of carbomer, 0.5 to 5 percent of polyvinyl alcohol, 0.1 to 1 percent of antibacterial component and 80 to 90 percent of deionized water;

preferably, the composition also comprises 0.05 to 1 percent of gelatin by mass percent;

preferably, the composition also comprises polyethylene glycol 1-10% by mass percent;

preferably, the composition also comprises 0.1 to 5 percent of sodium hyaluronate by mass percent;

preferably, the composition also comprises 0.1 to 5 percent of propylene glycol according to mass percent;

preferably, the composition also comprises 0.1-5% of sodium carboxymethyl cellulose by mass percent.

In an alternative embodiment, the composition further comprises 0.1-5% of active ingredient, wherein the active ingredient is at least one selected from laminin, extracellular matrix and growth factor;

preferably, the active ingredient comprises 0.1-2% laminin and 0.1-5% extracellular matrix;

preferably, the active ingredient comprises a growth factor of 0.05-1%.

In an alternative embodiment, the antibacterial component is 0.02-0.2% of nipagin ester, 0.01-1% of phenoxyethanol, 0.2-1% of p-hydroxyacetophenone and 0.1-1% of polyhexamethylene guanidine.

In a second aspect, the present application provides a method of preparing a hydrogel dressing as in the previous embodiments, comprising: the components contained in the hydrogel dressing are uniformly mixed to prepare the hydrogel.

In an alternative embodiment, the method comprises: the antibacterial component comprises phenoxyethanol, and the preparation method comprises the following steps:

uniformly mixing glycerol and deionized water to obtain a first mixture;

uniformly mixing the first mixture with the antibacterial component to obtain a second mixture;

uniformly mixing the second mixture with carbomer to obtain a third mixture;

uniformly mixing the third mixture with sodium alginate and collagen to obtain a fourth mixture;

uniformly mixing the solution of polyvinyl alcohol with the fourth mixture to obtain a fifth mixture;

the pH of the fifth mixture is adjusted to 5.0-7.5.

In an alternative embodiment, the pH adjuster used to adjust the pH of the fifth mixture is triethanolamine.

In an alternative embodiment, the mixing of each step is carried out at 50 to 300 rpm.

In an alternative embodiment, the components of the hydrogel dressing further comprise 0.1-5% of active components in percentage by mass, wherein the active components are selected from at least one of laminin, extracellular matrix removal and growth factors;

in the preparation process, a sixth mixture is obtained after the pH value of the fifth mixture is regulated, and the sixth mixture is uniformly mixed with the active ingredients;

preferably, the active ingredient comprises a growth factor of 0.05% -1%.

In an alternative embodiment, adjusting the pH of the fifth mixture further comprises:

the resulting mixture is sterilized by irradiation or moist heat.

The application has the following beneficial effects:

according to the hydrogel dressing and the preparation method thereof, the hydrogel dressing is formed by selecting proper amount of various components, and has excellent film forming property and moisture retention property, and remarkable wound healing promoting capability.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a photograph of a hydrogel dressing prepared in example 15;

FIG. 2 is a photograph showing comparison of hydrogel dressings prepared in example 16 and example 1;

FIG. 3 is a diagram showing the preparation process of example 17;

FIG. 4 is a photograph of the hydrogel dressing prepared in example 17;

FIGS. 5 to 8 are photographs showing the appearance of the hydrogel dressing according to Experimental example 2 after film formation;

FIG. 9 is an external view showing how the hydrogel dressing prepared in example 1 was applied to an affected part for 3 days in Experimental example 3.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present application more clear, the technical solutions of the embodiments of the present application will be clearly and completely described below. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.

The hydrogel dressing and the preparation method thereof provided by the embodiment of the application are specifically described below.

The embodiment of the application provides a hydrogel dressing, which comprises the following components in percentage by mass:

sodium alginate 0.5-5%, collagen 0.1-10%, glycerol 1-20%, carbomer 0.3-5%, polyvinyl alcohol 0.5-10%, antibacterial component 0.01-2%, deionized water 75-95%;

Collagen has the effect of promoting skin healing, and sodium alginate has the effects of stopping bleeding and promoting wound healing; the glycerol has the moisturizing effect, the water holding capacity of the hydrogel dressing can be ensured, the proper amount of the glycerol in the components can be favorable for the hydrogel dressing to form a layer of moist film, the hydrogel dressing can be well attached to the skin, the wound surface is protected, the hydrogel dressing without the glycerol forms a layer of dry film on the skin, and the moisture can be separated from the skin after evaporation; carbomer is used as a thickening agent and is used for improving the viscosity of the hydrogel to ensure the adhesive capacity of the hydrogel on a wound surface; the polyvinyl alcohol also has the function of a thickening agent, but more importantly, the polyvinyl alcohol is added into the components, so that the film forming performance of the hydrogel can be improved, a layer of protective film can be formed on a wound by the hydrogel dressing, the wound is covered, the wound is protected, a wet microenvironment is provided for the wound, the wound healing is accelerated, and in addition, the polyvinyl alcohol also has the function of surface activity.

The application selects the glycerol as the humectant, and the glycerol has excellent moisturizing performance and can maintain the gel viscosity after irradiation, and the gel without glycerol has lower viscosity after sterilization in the same proportion, so that the gel solution is thinner and is not easy to be uniformly smeared at a wound; the gel of the application has moderate viscosity after irradiation sterilization and is easy to be smeared at a wound.

Therefore, the hydrogel dressing is formed by selecting proper amounts of various components, has excellent film forming property and moisture retention property, and has remarkable capability of promoting wound healing.

Preferably, in order to enable the hydrogel dressing to have better performance, the hydrogel dressing comprises the following components in percentage by mass:

1 to 3 percent of sodium alginate, 0.5 to 5 percent of collagen, 3 to 10 percent of glycerol, 1 to 3 percent of carbomer, 0.5 to 5 percent of polyvinyl alcohol, 0.1 to 1 percent of antibacterial component and 80 to 90 percent of deionized water.

Preferably, the hydrogel dressing further comprises 0.05-1% of gelatin in percentage by mass.

Gelatin has excellent hemostatic property and good air permeability and water permeability, so that proper amount of gelatin is added into the hydrogel, and the gelatin, collagen and sodium alginate can be used for promoting the healing of wounds together so as to accelerate the healing of the wounds.

Preferably, the hydrogel dressing further comprises 1-10% of polyethylene glycol by mass percent.

Polyethylene glycol can block nerve, has good effect on indirect pain and persistent pain caused by nerve injury, and has certain promotion effect on wound healing. Polyethylene glycol, collagen and sodium alginate are used as matrix materials, and the substances act in different ways, so that the wound healing speed can be further accelerated.

Polyethylene glycol softens the polyethylene container, so that the container holding the hydrogel dressing cannot be the polyethylene container when polyethylene glycol is added to the hydrogel; in addition, polyethylene glycol also reduces the antibacterial effect of the parabens antibacterial agent, so that the parabens antibacterial agent cannot be added when polyethylene glycol is added to the hydrogel dressing, or the parabens preservative cannot be added when polyethylene glycol is added to the hydrogel dressing.

Preferably, the hydrogel dressing further comprises 0.1-5% of sodium hyaluronate by mass percent.

The sodium hyaluronate can promote proliferation and differentiation of epidermal cells and accelerate epidermal regeneration, so that wound healing is promoted, and the wound healing rate can be further improved by adding proper amount of sodium hyaluronate into the hydrogel dressing, and the sodium hyaluronate can also act as a humectant.

Preferably, the composition also comprises 0.1-5% of propylene glycol by mass percent. Propylene glycol also acts as a humectant.

Preferably, the hydrogel dressing further comprises 0.1-5% of sodium carboxymethyl cellulose by mass percent.

Sodium carboxymethylcellulose has a thickening and moisturizing effect similar to carbomers, and when added to a hydrogel dressing, the carbomers and polyvinyl alcohol can be suitably reduced.

Further, the hydrogel dressing comprises 0.1-5% of active ingredient by mass percent, wherein the active ingredient is at least one selected from laminin, extracellular matrix and growth factor.

The above active ingredients have effect in promoting cell proliferation and differentiation, and the hydrogel dressing can be added with appropriate amount of active ingredients to further increase wound healing rate.

Further, the active ingredients comprise 0.1 to 2 percent of laminin and 0.1 to 5 percent of extracellular matrix; the active component comprises 0.05-1% of growth factor.

Preferably, in order to further ensure the antibacterial effect of the hydrogel dressing, the antibacterial components in the hydrogel dressing are 0.02-0.2% of nipagin esters, 0.01-1% of phenoxyethanol, 0.2-1% of p-hydroxyacetophenone and 0.1-1% of polyhexamethylene guanidine.

The preparation method of the hydrogel dressing provided by the embodiment of the application comprises the following steps: the components contained in the hydrogel dressing are uniformly mixed to prepare the hydrogel.

The preparation method provided by the embodiment of the application is simple, and the hydrogel can be formed by uniformly mixing all the components.

In the existing hydrogel dressing preparation process, raw materials are directly mixed, however, the inventor researches that if the raw materials are directly mixed and stirred to prepare the hydrogel, foam and bubbles are generated in the process, and the raw materials which need to be heated and dissolved are unevenly dissolved, so that massive gel is easy to generate, and the product quality is seriously affected.

Preferably, in order to ensure the preparation efficiency of the hydrogel dressing and the quality of the prepared hydrogel dressing, the preparation method specifically comprises the following steps:

s1, preparing materials

Raw materials are prepared according to the proportion of each component of the hydrogel dressing, wherein the polyvinyl alcohol is dissolved by deionized water in advance, and the deionized water for dissolving the polyvinyl alcohol belongs to a part of 75-95% of deionized water in the hydrogel dressing.

To ensure uniform dispersion of the components, the following mixing steps are carried out at a rotation speed of 50 to 300rpm (e.g., 50rpm, 100rpm, 200rpm or 300 rpm).

S2, first mixing

Mixing glycerol and deionized water in a reaction kettle, and stirring for 5-10min (such as 5min, 8min and 10 min) to obtain a first mixture.

The sum of the deionized water used in this step and the deionized water dissolving the polyvinyl alcohol is the total amount of deionized water in the hydrogel dressing.

If sodium hyaluronate and propylene glycol are included in the composition, sodium hyaluronate and propylene glycol are mixed with glycerol and deionized water in this step.

S3, mixing for the second time

Adding antibacterial components including phenoxyethanol into a reaction kettle, and stirring for 5-10min (such as 5min, 8min and 10 min) to uniformly mix the phenoxyethanol with the first mixture in the reaction kettle to obtain a second mixture.

The inventor finds that the phenoxyethanol is added before carbomer, so that the foam generated by stirring when carbomer is added in the next step can be effectively avoided.

S4, third mixing

Adding carbomer into the reaction kettle, and stirring for 4-6h (4 h, 5h and 6 h) to uniformly mix the carbomer with the second mixture in the reaction kettle to obtain a third mixture.

Because carbomer has obvious thickening effect, if the carbomer is added later, the solute in the solution is more, the carbomer is not easy to dissolve, and the carbomer can be ensured to be dissolved faster by adding the carbomer when the solute in the solution is less, and the components can be dispersed more uniformly later.

If sodium carboxymethylcellulose is included in the composition, it is added to the reaction vessel along with carbomer in this step.

S5, fourth mixing

Adding sodium alginate and collagen into the reaction kettle, and stirring for 4-6h (such as 4h, 5h or 6 h) to uniformly mix the sodium alginate, the collagen and the third mixture to obtain a fourth mixture.

Sodium alginate is better dissolved than carbomer, the viscosity of the solution is higher after the carbomer is added into the solution, and the carbomer is not easy to dissolve if the carbomer is added after the sodium alginate.

If the components comprise gelatin or polyethylene glycol, the gelatin or polyethylene glycol, sodium alginate and collagen are added into a reaction kettle together, and the components are mixed in the step.

S6, mixing for the fifth time

Adding the prepared polyvinyl alcohol solution into the reaction kettle, and stirring for 1-2h to uniformly mix the polyvinyl alcohol solution with the fourth mixture to obtain a fifth mixture.

The polyvinyl alcohol is added in the form of a solution, so that the polyvinyl alcohol can be prevented from being insoluble or unevenly dissolved in the process of directly adding the polyvinyl alcohol into a reaction kettle at room temperature to prepare gel; the polyvinyl alcohol is added in the form of solution, so that the prepared hydrogel has good uniformity, and the whole preparation process period is shortened.

S7, pH adjustment

And adding triethanolamine into the fifth mixture to adjust the pH of the mixed system to 5.0-7.5 so that the pH of the hydrogel dressing is closer to that of a human body, thereby ensuring that the hydrogel dressing has better effect of promoting wound healing.

If the pH of the fifth mixture itself is in the range of 5.0 to 7.5, it is not necessary to add triethanolamine thereto to adjust the pH, and in this case, whether to add triethanolamine to adjust the pH may be selected according to the production requirements.

If the components also include active components, the step S7 is performed after:

s8, sixth mixing

Adding the active component into the reaction kettle, and stirring for 10-30min to uniformly mix the active component with the fifth mixture to obtain a sixth mixture.

S8, sterilizing

The fifth mixture or the sixth mixture is sterilized using irradiation or steam.

Preferably, the parameters of irradiation sterilization are 25-40KGy (e.g., 25KGy, 30KGy, or 40 KGy);

preferably, the parameters of steam sterilization may be generally 121 ℃,20min;

both radiation sterilization and damp heat sterilization are effective sterilization modes.

The features and capabilities of the present application are described in further detail below in connection with the examples.

Example 1

The embodiment of the application provides a hydrogel dressing and a preparation method thereof.

The hydrogel dressing comprises the following components:

sodium alginate 2%, collagen 1%, carbomer 1%, polyvinyl alcohol 1%, phenoxyethanol 0.5%, glycerol 8%, polyethylene glycol 1%, sodium hyaluronate 1%, laminin 0.5%, extracellular matrix removal 0.5%, growth factor 0.2% and the balance deionized water.

The preparation method comprises the following steps:

dissolving polyvinyl alcohol by using part of deionized water to obtain a polyvinyl alcohol solution;

starting a stirring device of the reaction kettle, and setting the rotating speed to be 100rpm;

mixing and stirring glycerol, sodium hyaluronate and the rest deionized water in a reaction kettle for 5min to uniformly mix the components;

adding phenoxyethanol into a reaction kettle, mixing and stirring for 5min to uniformly mix the components;

adding carbomer and sodium carboxymethyl cellulose into a reaction kettle, mixing and stirring for 5 hours to uniformly mix the components;

adding sodium alginate, collagen and gelatin into a reaction kettle, mixing and stirring for 5 hours to uniformly mix the components;

adding a pre-prepared polyvinyl alcohol solution into a reaction kettle, mixing and stirring for 1h to uniformly mix the components;

a small amount of triethanolamine was slowly added to the reaction vessel to bring the pH of the mixture in the reaction vessel to about 6.5.

Adding laminin and extracellular matrix removal into a reaction kettle, mixing and stirring for 30min to uniformly mix the components;

and (3) uniformly mixing to obtain a mixture, and carrying out irradiation sterilization, wherein the irradiation parameter is 25KGy, so as to obtain the hydrogel dressing.

Example 2

The hydrogel dressing comprises the following components:

sodium alginate 0.5%, collagen 10%, glycerol 1%, carbomer 5%, polyvinyl alcohol 1%, nipagin ester 0.02%, polyhexamethylene guanidine 0.1%, sodium hyaluronate 0.1%, sodium carboxymethylcellulose 0.1%, laminin 0.1% and the balance water.

The preparation method is the same as in example 1.

Example 3

The hydrogel dressing comprises the following components:

sodium alginate 5%, collagen 0.1%, glycerol 15%, carbomer 1%, polyvinyl alcohol 1%, phenoxyethanol 0.5%, glycerol 1%, gelatin 0.1%, polyethylene glycol 1%, sodium hyaluronate 0.1%, extracellular matrix removal 0.1% and the balance water.

The preparation method is the same as in example 1.

Example 4

The hydrogel dressing comprises the following components:

sodium alginate 1%, collagen 2%, glycerol 1%, carbomer 2.5%, polyvinyl alcohol 10%, polyhexamethylene guanidine 0.5%, sodium carboxymethylcellulose 0.1%, extracellular matrix 0.1% and the balance water.

The preparation method is the same as in example 1.

Example 5

The hydrogel dressing comprises the following components:

sodium alginate 1.5%, collagen 5%, glycerol 2%, carbomer 0.3%, polyvinyl alcohol 1%, phenoxyethanol 0.01%, p-hydroxyacetophenone 0.2%, polyethylene glycol 1%, extracellular matrix removal 0.1% and the balance water.

The preparation method is the same as in example 1.

Example 6

The hydrogel dressing comprises the following components:

sodium alginate 0.5%, collagen 0.5%, glycerol 15%, carbomer 2%, polyvinyl alcohol 1%, nipagin ester 0.02%, sodium carboxymethylcellulose 0.1%, extracellular matrix removed 0.1% and the balance water.

The preparation method is the same as in example 1.

Example 7

The hydrogel dressing comprises the following components:

sodium alginate 0.5%, collagen 0.5%, glycerol 2%, carbomer 1%, polyvinyl alcohol 10%, phenoxyethanol 0.01%, polyhexamethylene guanidine 0.1%, p-hydroxyacetophenone 0.2%, polyethylene glycol 1%, sodium carboxymethylcellulose 0.1%, extracellular matrix removal 0.1% and the balance water.

The preparation method is the same as in example 1.

Example 8

The hydrogel dressing comprises the following components:

sodium alginate 2.5%, collagen 0.5%, glycerol 5%, carbomer 0.3%, polyvinyl alcohol 1%, phenoxyethanol 0.01%, polyhexamethylene guanidine 0.1%, p-hydroxyacetophenone 0.2%, glycerol 5%, polyethylene glycol 10%, extracellular matrix removal 0.1% and the balance water.

The preparation method is the same as in example 1.

Example 9

The hydrogel dressing comprises the following components:

sodium alginate 2%, collagen 2%, glycerol 5%, carbomer 0.5%, polyvinyl alcohol 2%, phenoxyethanol 0.01%, p-hydroxyacetophenone 0.2%, polyethylene glycol 4%, sodium carboxymethylcellulose 5%, extracellular matrix 0.1% and the balance water.

The preparation method is the same as in example 1.

Example 10

The hydrogel dressing comprises the following components:

sodium alginate 2%, collagen 2%, glycerol 1%, carbomer 0.3%, polyvinyl alcohol 2%, phenoxyethanol 0.01%, polyhexamethylene guanidine 0.1%, p-hydroxyacetophenone 0.2%, polyethylene glycol 1%, sodium hyaluronate 5%, sodium carboxymethylcellulose 5%, extracellular matrix 0.1% and the balance water.

The preparation method is the same as in example 1.

Example 11

The hydrogel dressing comprises the following components:

sodium alginate 2%, collagen 2%, propylene glycol 1%, glycerol 1%, carbomer 0.3%, polyvinyl alcohol 2%, p-hydroxyacetophenone 0.2%, polyethylene glycol 1%, gelatin 4%, sodium hyaluronate 1%, sodium carboxymethylcellulose 1%, extracellular matrix 0.1% and the balance water.

The preparation method is the same as in example 1.

Example 12

The same ingredients as in example 1 were prepared, with the exception that the following ingredients were used in amounts:

sodium alginate 3%, collagen 2%, carbomer 1.5%, polyvinyl alcohol 3%, phenoxyethanol 0.5%, glycerol 5%, polyethylene glycol 2%, sodium hyaluronate 1%, laminin 0.5%, extracellular matrix 0.5% and the balance deionized water.

Example 13

sodium alginate 1%, collagen 4%, carbomer 2%, polyvinyl alcohol 0.5%, phenoxyethanol 0.5%, glycerol 7%, polyethylene glycol 5%, sodium hyaluronate 1%, laminin 0.5%, extracellular matrix 0.5%, growth factor 0.1% and the balance deionized water.

Example 14

sodium alginate 1.5%, collagen 2%, carbomer 1%, polyvinyl alcohol 3%, phenoxyethanol 0.2%, glycerol 86%, polyethylene glycol 1%, sodium hyaluronate 2%, laminin 0.5%, extracellular matrix 0.5%, growth factor 0.1% and the balance deionized water.

Example 15

This embodiment is substantially the same as embodiment 1, except that:

the individual components are mixed directly.

The mixing process is carried out with bubble generation, resulting in poor uniformity of the hydrogel dressing produced. As shown in FIG. 1, FIG. 1 is a photograph of a hydrogel obtained in this example, which has lumps and is uneven.

Example 16

This embodiment is substantially the same as embodiment 1, except that:

in the preparation process, the polyvinyl alcohol is not prepared into solution in advance, but is directly added into a reaction kettle to be mixed with other materials.

As shown in fig. 2, the hydrogel a prepared in this example was extruded in a block shape and was not uniform; the uniformly dispersed hydrogel b prepared in comparative example 1 illustrates that it is difficult to uniformly disperse polyvinyl alcohol directly into a reaction kettle at normal temperature.

Example 17

This embodiment is substantially the same as embodiment 1, except that:

the order of addition of phenoxyethanol and carbomer was reversed.

As shown in fig. 3, more foam was generated after adding a carbomer, and b, although foam was lost after adding phenoxyethanol, this resulted in the formation of lumps during the mixing process, and further resulted in poor uniformity of the resulting gel, as shown in fig. 4, and fig. 4 is a photograph of the hydrogel material obtained in this example, with lumps in the gel.

Comparative example 1

This comparative example is substantially the same as example 1, except that no glycerol is contained in the components.

Comparative example 2

This comparative example is substantially the same as example 1 except that the polyvinyl alcohol is not contained in the component.

Experimental example 1

The viscosities of example 1 and the hydrogel dressings prepared in comparative example 1 were tested as shown in table 1.

TABLE 1 viscosity statistics

Group of	viscosity/mPas
		Comparative example 1	5560
Example 1	22750

From the above table, the viscosity of the hydrogel dressing without glycerol is significantly less.

Experimental example 2

The hydrogel dressings prepared in example 1, comparative example 1 and comparative example 2 were applied to a human hand, and the external appearance thereof was as shown in fig. 5 to 8.

Fig. 5 is a photograph of the hydrogel dressing prepared in example 1 applied to the back of a human hand after evaporation of water, and fig. 6 is a photograph of the dressing of example 1 torn off after film formation. As can be seen from fig. 3 and 4, the hydrogel dressing prepared in the embodiment of the present application is a wet film after being formed into a film, is fully transparent, provides an excellent wet environment for wound healing, and is obviously more beneficial to wound healing.

Fig. 7 is a photograph of the hydrogel dressing prepared in comparative example 1 applied to the back of a human hand after evaporation of water, and fig. 8 is a photograph of the hydrogel dressing prepared in comparative example 2 applied to the back of a human hand after evaporation of water. It can be seen from fig. 7 that the film without glycerol forms a dry film, which is clearly detrimental to wound healing. As can be seen from fig. 8, it is difficult to form a complete film after evaporation of the water after application of the hydrogel dressing without polyvinyl alcohol.

Experimental example 3

The hydrogel dressing prepared in example 1 was applied to a wound site, and the wound was photographed after 0 and 3 days, as shown in fig. 9.

As can be seen from fig. 9, the hydrogel dressing prepared in example 1 has a remarkable promoting effect on wound healing.

In summary, the hydrogel dressing and the preparation method thereof provided by the application form the hydrogel dressing by selecting proper amount of various components, and the hydrogel dressing has excellent film forming property and moisture retention property and has remarkable capability of promoting wound healing.

In the preferred embodiment of the application, a special preparation method is adopted, so that foam generation in the preparation process is avoided, the quality of the prepared hydrogel dressing can be improved, meanwhile, as no foam is generated in the preparation process, the preparation process can be ensured to have shorter time, the production efficiency of enterprises is ensured, and the economic benefit of the enterprises is further ensured.

The above is only a preferred embodiment of the present application, and is not intended to limit the present application, but various modifications and variations can be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. The preparation method of the hydrogel dressing is characterized by comprising the following components in percentage by mass:

0.5-5% of sodium alginate, 0.1-10% of collagen, 1-20% of glycerol, 0.3-5% of carbomer, 0.5-10% of polyvinyl alcohol, 0.01-2% of antibacterial component and 75-95% of deionized water;

the antibacterial component is phenoxyethanol;

the preparation method comprises the following steps: uniformly mixing the glycerol and deionized water to obtain a first mixture;

uniformly mixing the second mixture with the carbomer to obtain a third mixture;

uniformly mixing the third mixture with the sodium alginate and the collagen to obtain a fourth mixture;

uniformly mixing the solution of the polyvinyl alcohol with the fourth mixture to obtain a fifth mixture;

and adjusting the pH value of the fifth mixture to 5.0-7.5.

2. The method of claim 1, wherein the hydrogel dressing composition comprises, in mass percent: 1-3% of sodium alginate, 0.5-5% of collagen, 3-10% of glycerol, 1-3% of carbomer, 0.5-5% of polyvinyl alcohol, 0.1-1% of antibacterial components and 80-90% of deionized water.

3. The preparation method of claim 2, further comprising 0.01-2% of gelatin by mass percent.

4. The preparation method of claim 2, further comprising 1-10% of polyethylene glycol by mass percent.

5. The preparation method of claim 2, further comprising 0.1-5% by mass of sodium hyaluronate.

6. The preparation method of claim 2, further comprising 0.1-5% by mass of propylene glycol.

7. The preparation method of claim 2, further comprising 0.1-5% by mass of sodium carboxymethylcellulose.

8. The preparation method according to claim 1, further comprising 0.1-5% by mass of an active ingredient selected from at least one of laminin, extracellular matrix and growth factor.

9. The method of claim 8, wherein the active ingredient comprises 0.05% -1% growth factor.

10. The method according to claim 1, wherein the pH adjuster used for adjusting the pH of the fifth mixture is triethanolamine.

11. The method of claim 1, wherein the mixing in each step is performed at 50 to 300 rpm.

12. The preparation method of claim 1, wherein the components of the hydrogel dressing further comprise 0.1-5% by mass of an active ingredient selected from at least one of laminin, extracellular matrix and growth factor;

in the preparation process, a sixth mixture is obtained after the pH of the fifth mixture is regulated, and the sixth mixture is uniformly mixed with the active ingredient.

13. The method of preparing according to claim 1, further comprising, after adjusting the pH of the fifth mixture:

the resulting mixture is sterilized by irradiation or moist heat.