CN115349636A - Whitening and spot-fading composition containing cubilose peptide and preparation method and application thereof - Google Patents

Abstract

The present invention relates to the technical field of food processing, in particular to a whitening composition containing bird's nest peptide and its preparation method and application, wherein the whitening composition includes the following raw materials by weight fraction: 0.1-0.3 parts of bird's nest peptide , 4-6 parts of fish collagen peptide, 1.5-4 parts of Cordyceps militaris, 2.5-5 parts of burdock root, 2-8 parts of Bao Le fruit powder, 0.5-3 parts of sea buckthorn fruit powder and 0.5-3 parts of stevia leaf. In the present invention, the whitening and lightening composition prepared by compounding fish collagen peptide, Cordyceps militaris, burdock root, Baole fruit powder, seabuckthorn fruit powder and stevia leaf with bird's nest peptide not only enriches the product types and efficacy of bird's nest, Moreover, it has the ability to essentially solve the problem of facial pigmentation deposition, remove free radicals and peroxides in the skin, inhibit tyrosinase activity, organize tyrosine hydroxylation, and reduce the generation of melanin, thereby effectively removing or It has the effect of lightening facial spots and whitening skin.

Description

A whitening composition containing bird's nest peptide and its preparation method and application

technical field

The invention relates to the technical field of food processing, in particular to a whitening composition containing bird's nest peptide and its preparation method and application.

Background technique

In modern society, there are generally high work pressure, fast pace of life, irregular diet, and long-term exposure to radiation such as mobile phones, computers, and tablets every day, which leads to skin problems such as yellowish and dark skin and obvious pigmentation in the social population, which have affected people's beauty. mood and quality of life.

At present, many female patients spend a lot of time, energy and money in order to cure skin problems such as pigmentation and whitening, but the treatment effect is often not very good. For example, many cosmetics contain a lot of heavy metal ions, long-term use will cause damage to human health; and some western medicine products may contain a large amount of hormones, heavy metals, etc., which have brought great harm to human health.

According to traditional medicine, the skin color of the face is closely related to the qi and blood of the five internal organs, and beauty must start from the whole. The diseases that affect the facial beauty of the human body mainly include freckles, chloasma, acne, skin pigmentation, etc. The cause of these symptoms is mainly due to the increased formation of melanin. major facilitation. Although it has become a part of women's daily life to protect and beautify the skin by using skin care products and cosmetics, more and more women realize that only by using skin care products and cosmetics can not really protect and beautify the skin, only from the inside , in order to have a ruddy complexion. Therefore, it has increasingly become the consensus of most women to take whitening and freckle-removing drinks to achieve beauty from the inside out.

Bird's nest is a nest made by mixing and coagulating the saliva and fluff secreted by swiftlets of the Swift family Swiftlet and a variety of swallows belonging to the same family. It is mainly produced in Southeast Asian countries and the South my country Sea islands. Bird's nest is sweet in taste and flat in nature, and has the effects of nourishing the lungs, nourishing the stomach, nourishing qi and nourishing the kidneys. At the same time, its effect of nourishing the middle and nourishing qi has a significant effect on physical weakness and post-illness conditioning. It also has obvious relieving effects on symptoms such as easy sweating, frequent urination, and frequent nocturia caused by qi deficiency in middle-aged and elderly people. The main nutrients of bird's nest include water-soluble protein, carbohydrates, trace elements (including calcium, magnesium, sodium and potassium) and amino acids that play an important role in the human body, such as aspartic acid and serine, among which sialic acid is the most potent in bird's nest. One of the components of value.

However, there is no whitening and spot-lightening product containing bird's nest in the prior art. Therefore, in response to the current market demand for whitening and spot-lightening drinks, a medicine-food homologous product with both nourishing and skin-whitening and spot-lightening effects of bird's nest is developed. Beverage is a technical problem that those skilled in the art need to solve urgently.

Contents of the invention

The purpose of the present invention is to provide a whitening composition containing bird's nest peptide and its product. The product is a whitening drink with the same source of medicine and food, which has the effect of effectively removing or lightening facial spots and whitening the skin.

The invention provides a whitening composition containing bird's nest peptide, which comprises the following raw materials in weight fraction:

Bird's nest peptide 0.1-0.3 parts, fish collagen peptide 4-6 parts, Cordyceps militaris 1.5-4 parts, burdock root 2.5-5 parts, Bao Le fruit powder 2-8 parts, seabuckthorn fruit powder 0.5-3 parts and stevia leaf 0.5- 3 copies.

Through actual preparation and research on its efficacy, the better technical solutions selected are as follows:

Bird's nest peptide 1mg/ml, Cordyceps militaris 20mg/ml, burdock root 30mg/ml, Baole fruit powder 30mg/ml, seabuckthorn fruit powder 10mg/ml, stevia leaf 5mg/ml;

Bird's nest peptide 2mg/ml, Cordyceps militaris 20mg/ml, burdock root 30mg/ml, Baole fruit powder 30mg/ml, seabuckthorn fruit powder 10mg/ml, stevia leaf 5mg/ml;

Bird's nest peptide 3mg/ml, Cordyceps militaris 20mg/ml, burdock root 30mg/ml, Baole fruit powder 30mg/ml, seabuckthorn fruit powder 10mg/ml, stevia leaf 5mg/ml;

Bird's nest peptide 1mg/ml, Cordyceps militaris 15mg/ml, burdock root 45mg/ml, Baole fruit powder 45mg/ml, seabuckthorn fruit powder 15mg/ml, stevia leaf 10mg/ml;

Bird's nest peptide 1mg/ml, Cordyceps militaris 20mg/ml, burdock root 45mg/ml, Baole fruit powder 45mg/ml, seabuckthorn fruit powder 15mg/ml, stevia leaf 10mg/ml;

Bird's nest peptide 1mg/ml, Cordyceps militaris 40mg/ml, burdock root 45mg/ml, Baole fruit powder 45mg/ml, seabuckthorn fruit powder 15mg/ml, stevia leaf 10mg/ml;

Bird's nest peptide 1mg/ml, Cordyceps militaris 30mg/ml, burdock root 30mg/ml, Baole fruit powder 60mg/ml, seabuckthorn fruit powder 20mg/ml, stevia leaf 15mg/ml;

Bird's nest peptide 1mg/ml, Cordyceps militaris 30mg/ml, burdock root 45mg/ml, Baole fruit powder 60mg/ml, seabuckthorn fruit powder 20mg/ml, stevia leaf 15mg/ml;

Bird's nest peptide 1mg/ml, Cordyceps militaris 30mg/ml, burdock root 60mg/ml, Baole fruit powder 60mg/ml, seabuckthorn fruit powder 20mg/ml, stevia leaf 15mg/ml;

Bird's nest peptide 1mg/ml, fish collagen peptide 40mg/ml, Cordyceps militaris 20mg/ml, burdock root 30mg/ml, Baole fruit powder 30mg/ml, seabuckthorn fruit powder 10mg/ml, stevia leaf 5mg/ml;

Bird's nest peptide 1mg/ml, fish collagen peptide 50mg/ml, Cordyceps militaris 20mg/ml, burdock root 30mg/ml, Baole fruit powder 30mg/ml, seabuckthorn fruit powder 10mg/ml, stevia leaf 10mg/ml;

Bird's nest peptide 1mg/ml, fish collagen peptide 60mg/ml, Cordyceps militaris 20mg/ml, burdock root 30mg/ml, Baole fruit powder 30mg/ml, seabuckthorn fruit powder 10mg/ml, stevia leaf 15mg/ml;

Bird's nest peptide 1mg/ml, fish collagen peptide 50mg/ml, Cordyceps militaris 20mg/ml, burdock root 45mg/ml, Baole fruit powder 45mg/ml, seabuckthorn fruit powder 10mg/ml, stevia leaf 10mg/ml;

Bird's nest peptide 1mg/ml, fish collagen peptide 50mg/ml, Cordyceps militaris 20mg/ml, burdock root 45mg/ml, Baole fruit powder 45mg/ml, seabuckthorn fruit powder 15mg/ml, stevia leaf 10mg/ml;

Bird's nest peptide 1mg/ml, fish collagen peptide 50mg/ml, Cordyceps militaris 20mg/ml, burdock root 45mg/ml, Baole fruit powder 45mg/ml, seabuckthorn fruit powder 20mg/ml, stevia leaf 10mg/ml;

Bird's nest peptide 1mg/ml, fish collagen 50mg/ml, Cordyceps militaris 30mg/ml, burdock root 60mg/ml, Baole fruit powder 60mg/ml, seabuckthorn fruit powder 20mg/ml, stevia leaf 5mg/ml;

Bird's nest peptide 1mg/ml, fish collagen 50mg/ml, Cordyceps militaris 30mg/ml, burdock root 60mg/ml, Baole fruit powder 60mg/ml, seabuckthorn fruit powder 20mg/ml, stevia leaf 10mg/ml;

Bird's nest peptide 1mg/ml, fish collagen 50mg/ml, Cordyceps militaris 30mg/ml, burdock root 60mg/ml, Baole fruit powder 60mg/ml, seabuckthorn fruit powder 20mg/ml, stevia leaf 15mg/ml.

Preferably, as the technical solution, the content of sialic acid in the bird's nest peptide is 10-11%.

The bird’s nest peptide in this application selects the bird’s nest peptide prepared by the bird’s nest compound enzymatic hydrolysis process as a raw material, and controls the content of sialic acid in the bird’s nest peptide within the range of 10-11%, so as to maintain the beauty of the bird’s nest itself, improve immunity and delay aging, etc. effect.

The present invention also discloses a preparation method of the above-mentioned whitening and blemish-relieving composition, which includes the following steps:

S1. Separately pulverize Cordyceps militaris, burdock root and stevia leaves and decoct and extract them several times to obtain Cordyceps militaris extract, burdock root extract and stevia leaf extract;

S2. Mixing the Cordyceps militaris extract, the burdock root extract, the stevia leaf extract and the remaining raw materials by weight to obtain a whitening and spot-reducing composition.

In order to further improve the efficacy of the whitening composition, the present invention selects the method of decocting and extracting the active ingredients in Cordyceps militaris, burdock root and stevia leaf, because the active ingredients in Cordyceps militaris, burdock root and stevia leaf will be different. Therefore, the active ingredient can be obtained by decocting and extracting, which reduces the problem of solvent pollution and solvent residue caused by the use of chemical organic solvent extraction, and ensures the food safety of the composition.

The present invention does not strictly limit the specific times of decoction and extraction and the amount of water used. It is advisable to reduce production and processing costs under the premise of fully and effectively extracting active ingredients. Specifically, the decoction extraction method specifically includes the following steps:

Crush the raw materials to 40-60 mesh, add water to decoct and extract, filter the medicinal liquid after each decoction, keep the medicinal residues, and finally combine and filter the medicinal liquid obtained by each decoction to obtain the extract;

When decocting each time, the amount of water used is 15-30 times the quality of the raw material, and each time decocting until the medicinal liquid is lost by 1/3-1/2; the number of decoctions is 3-4 times.

Regarding the application of the whitening and blemish-reducing composition, the present invention also provides a whitening and blemish-reducing solid beverage comprising the above-mentioned whitening and blemish-reducing composition, specifically, the whitening and blemish-reducing composition is sequentially concentrated and freeze-dried to obtain a whitening and blemish-reducing composition solid drink.

Preferably, as the technical solution, during the concentration, the whitening and blemish-reducing composition is concentrated to 0.9-1.1 times the total weight of raw materials.

In addition, the present invention also provides a whitening and blemish-reducing oral liquid comprising the above-mentioned whitening and blemish-reducing composition. Specifically, the whitening and blemish-reducing composition is filled, sealed, sterilized and packaged sequentially to obtain the whitening and blemish-reducing oral solution. liquid.

The present invention does not strictly limit the specific conditions of filling, sealing and sterilization. Specifically, during the filling, the filling temperature can be controlled to be ≥60°C; during the sealing, the sealing torque can be controlled to be 0.5-1.5Nm; During the above sterilization, the controllable temperature is 105-115°C and the time is 15-30min.

The role of each component in this application:

Fish collagen peptide: Deep-sea fish collagen peptide is preferred, which can supplement the collagen in the skin that decreases with age, restore the elasticity and tension of the skin, keep the skin moist and smooth, promote the proliferation of skin fibroblasts, and maintain Skeletal support of the skin. In addition, the deep-sea fish collagen peptide molecule is less than 1000D, which is easy to absorb. Oral and easy-to-absorb collagen peptide can specifically act on collagen fibers, such as Achilles tendon and dermis, thereby inhibiting skin aging and keeping the skin white and smooth.

Cordyceps militaris: Sugar, fat and protein are the three major nutritional elements of Cordyceps militaris. In addition, Cordyceps militaris also contains a variety of amino acids, trace elements and vitamins. The main active ingredients are cordycepin, adenosine and cordycepic acid (Mannitol), the content is basically the same as that of Cordyceps sinensis.

In this application, the lightening and whitening effect of Cordyceps militaris is reflected in the following aspects: (1) Cordyceps polysaccharide, the main chemical component of Cordyceps militaris, has a protective effect on skin photoaging, and can also affect the synthesis of collagen in skin fibroblasts , significantly increased the antioxidant capacity of skin fibroblasts; (2) Cordyceps militaris is a cellular immune regulator, but also has humoral immune regulation, and plays a multi-link regulation or inhibitory role in the immune pathological process of the body; (3) pupae Cordyceps has obvious anti-aging effect.

Burdock root: sweet in taste and cool in nature, it has the functions of clearing lung and relieving cough, clearing heat and detoxification, clearing liver and calming wind, softening hard masses and resolving stagnation, moistening intestines and laxative. Studies have shown that burdock root has antibacterial, anti-oxidant, hypoglycemic, hypolipidemic, vasodilation, improvement of atherosclerosis, anti-tumor, anti-mutation and immune regulation effects.

The lightening and whitening effect of burdock root in this application is reflected in the following aspects: (1) burdock root is rich in dietary fiber, which can increase the peristalsis of the intestinal tract, enhance the ability of eliminating toxins in the body, and achieve the effect of detoxification and beauty. At the same time, dietary fiber has the effect of absorbing sodium, and can be excreted with feces, so that the content of sodium in the body is reduced, thereby achieving the purpose of lowering blood pressure. Normal blood pressure helps to maintain the vitality of skin metabolism; (2) Burdock root The content of calcium is the highest among root vegetables. Calcium can ensure the normal metabolism and operation of each cell in the body, reduce physical fatigue, and keep the skin healthy; (3) The protein content in burdock root is high, and protein can Make blood vessels flexible, increase the function of dermal blood vessels to transport nutrients and eliminate metabolic waste, promote skin whitening and reduce pigmentation; (4) arctiin contained in burdock root can dilate blood vessels and enhance skin nutrition Supply and hydration.

Therefore, burdock root can clean up blood garbage, promote the metabolism of cells in the body, increase skin nutrition and water replenishment, prevent aging, make skin firm and delicate, and have the effect of removing yellow and reducing pigmentation.

Seabuckthorn: Seabuckthorn has the functions of improving immunity, protecting the liver, lowering cholesterol, relieving cough and asthma, invigorating stomach and eliminating food, improving eyesight and reducing inflammation. Seabuckthorn is rich in vitamin C, which can reduce skin pigmentation and skin aging caused by oxidative stress, and enhance the activity of skin cells. The fruit components contained in seabuckthorn can inhibit the inflammatory response of the skin. Other ingredients in seabuckthorn can help remove excess toxins and garbage in pores, reduce the deposition of melanin and excessive oil secretion, shrink pores, and refine the skin.

Bao Le Guo: Contains many nitrogen-containing amino acid derivatives that play an important role in the process of human nutrition metabolism, such as aspartic acid, glutamine, glycine, taurine, cysteine, glutamic acid and glycine; γ-aminobutyric acid is an important nerve medium in the human body; it contains essential trace elements such as zinc, copper, iron, etc.; it is rich in vitamins B1 and B2, niacin, vitamin B9 and vitamin C. Its main active substances are gallic acid, polyphenols, polysaccharides, etc., which have the functions of anti-ultraviolet damage, anti-oxidation, anti-aging, and enhancing immunity in the body. Not only can it inhibit the damage of oxidative stress to the skin, but the bioactive ingredients contained in it can resist the damage of photoaging to the skin, inhibit the increase of intracellular tyrosinase, block the formation and deposition of pigment, whiten the skin and effectively reduce the sun zone various skin problems.

Stevia leaf: It has pharmacological activities such as anti-oxidation, antibacterial, anti-virus, anti-tumor, and immune regulation. The stevioside contained in stevia leaves has good sweetness, no fat, no caries, and high safety. It not only plays a flavoring role in the formula, but also has an inhibitory effect on the oxidative stress that causes skin pigmentation and skin aging.

The whitening and lightening composition of the present invention has at least the following technical effects:

1. In the present invention, the whitening and lightening composition prepared by compounding fish collagen peptide, Cordyceps militaris, burdock root, Baole fruit powder, seabuckthorn fruit powder and stevia leaf with bird's nest peptide not only enriches the product types of bird's nest and Efficacy, and it can essentially solve the problem of facial pigmentation deposition, remove free radicals and peroxides in the skin, and inhibit tyrosinase activity, organize tyrosine hydroxylation, reduce melanin production, and effectively The effect of removing or lightening facial pigmentation and whitening the skin, and the composition of the present application has excellent anti-glycation effect, and has inhibitory effect on the excessive generation of AGEs in skin tissue, thereby maintaining the elasticity of the skin, delaying skin aging, and reducing the appearance of wrinkles. At the same time, because the increase of AGEs is inhibited, the secretion of melanin in the skin is reduced, which in turn plays the role of keeping the skin fair and clear, whitening and yellowing;

2. In the whitening composition and its products prepared by the present invention, whether it is bird’s nest peptide, or fish collagen peptide, or Cordyceps militaris extract, burdock root extract, Baole fruit powder, seabuckthorn fruit powder and stevia leaf extract All substances are small molecular substances, which are easy to be absorbed by the human body, and can better exert the effect of whitening and lightening spots;

3. The components of the whitening and blemish-reducing composition prepared by the present invention are synergistic, and have good cosmetic effects such as improving skin microcirculation, whitening and even skin tone.

Description of drawings

In order to more clearly illustrate the specific implementation of the present invention or the technical solutions in the prior art, the following will briefly introduce the accompanying drawings that need to be used in the specific implementation or description of the prior art. Obviously, the accompanying drawings in the following description The drawings show some implementations of the present invention, and those skilled in the art can obtain other drawings based on these drawings without any creative work.

Fig. 1 is the measurement of DPPH free radical scavenging ability of comparative example 1 of the present invention whitening solid beverage lyophilized sample;

Fig. 2 is the determination of the scavenging ability of ABTS+ free radicals by the freeze-dried sample of the whitening and lightening solid beverage of Comparative Example 1 of the present invention;

Fig. 3 is the ORAC antioxidant test result of the freeze-dried samples of whitening and lightening solid beverage samples of Example 1 of the present invention and Comparative Example 1;

Fig. 4 is the inhibitory effect of β-arbutin of the present invention to tyrosinase;

Fig. 5 is the inhibition of tyrosinase by the freeze-dried samples of the whitening and lightening solid beverage of Example 1 of the present invention and Comparative Example 1;

Fig. 6 is the inhibition rate of AGEs formation in the BSA-fructose reaction system of the freeze-dried samples of the whitening and lightening solid beverage of Example 1 of the present invention and Comparative Example 1;

Fig. 7 shows the inhibition rate of AGEs formation in the BSA-MGO model reaction system of the freeze-dried samples of the whitening and lightening solid beverage of Example 1 and Comparative Example 1 of the present invention.

Detailed ways

It should be pointed out that the following detailed description is exemplary and intended to provide further explanation to the present application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

It should be noted that the terminology used here is only for describing specific implementations, and is not intended to limit the exemplary implementations according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form also includes the plural form, and it should also be understood that when the terms "comprising" and/or "comprising" are used in this description, it indicates that there are Features, steps, operations, devices, components and/or combinations thereof.

The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

Example 1

Bird's nest peptide 0.2g, fish collagen peptide 5g, Cordyceps militaris 3g, burdock root 4g, Baole fruit powder 5g, seabuckthorn fruit powder 2g and stevia leaves 2g.

1. Preparation of whitening and lightening composition

Grind Cordyceps militaris, burdock root and stevia leaves to 40-60 mesh respectively, add water to decoct and extract 3 times, filter the medicinal liquid after each decoction, keep medicinal residues, and finally combine the medicinal liquid obtained each time Filter to obtain the extract; each decoction, the amount of water used is 20 times the quality of the raw material, and each decoction until the liquid medicine is lost by 1/2;

The extract of Cordyceps militaris, the extract of burdock root and the extract of stevia leaf are mixed with the remaining weight fraction of raw materials to obtain the composition for whitening and lightening spots.

2. Preparation of whitening and lightening solid drink

The whitening and blemish-reducing composition is concentrated to one time of the total weight of raw materials, and freeze-dried to obtain a whitening and blemish-reducing solid beverage.

3. Preparation of Whitening and Spotless Oral Liquid

The whitening composition is filled, sealed, sterilized, and packaged sequentially. When filling, the filling temperature is controlled to be ≥60°C; when sealing, the sealing torque is controlled to be 1.0Nm; The time is 20 minutes, and finally the oral liquid for whitening and blemishes is obtained.

Example 2

Bird's nest peptide 0.1g, fish collagen peptide 4g, Cordyceps militaris 1.5g, burdock root 2.5g, Baole fruit powder 2g, seabuckthorn fruit powder 0.5g and stevia leaf 0.5g.

1. Preparation of whitening and lightening composition

Grind Cordyceps militaris, burdock root and stevia leaves to 40-60 mesh respectively, add water to decoct and extract 3 times, filter the medicinal liquid after each decoction, keep medicinal residues, and finally combine the medicinal liquid obtained each time Filter to obtain the extract; each decoction, the amount of water used is 15 times the quality of the raw material, and each decoction until the liquid medicine is lost by 1/2;

The extract of Cordyceps militaris, the extract of burdock root and the extract of stevia leaf are mixed with the remaining weight fraction of raw materials to obtain the composition for whitening and lightening spots.

2. Preparation of whitening and lightening solid drink

The whitening and blemish-reducing composition is concentrated to 1.0 times the total weight of raw materials, and freeze-dried to obtain a whitening and blemish-reducing solid beverage.

3. Preparation of Whitening and Spotless Oral Liquid

The whitening composition is filled, sealed, sterilized, and packaged sequentially. When filling, the filling temperature is controlled to be ≥60°C; when sealing, the sealing torque is controlled to be 1.0Nm; The time is 15 minutes, and finally the whitening and blemish-reducing oral liquid is obtained.

Example 3

Bird's nest peptide 0.3g, fish collagen peptide 6g, Cordyceps militaris 4g, burdock root 5g, Baole fruit powder 8g, seabuckthorn fruit powder 3g and stevia leaves 3g.

1. Preparation of whitening and lightening composition

Grind Cordyceps militaris, burdock root and stevia leaves to 40-60 mesh respectively, add water to decoct and extract 3 times, filter the medicinal liquid after each decoction, keep medicinal residues, and finally combine the medicinal liquid obtained each time Filtrate to obtain the extract; each decoction, the water used is 30 times the quality of the raw material, and each decoction until the liquid medicine is lost by 1/2;

The extract of Cordyceps militaris, the extract of burdock root and the extract of stevia leaf are mixed with the remaining weight fraction of raw materials to obtain the composition for whitening and lightening spots.

2. Preparation of whitening and lightening solid drink

The whitening and blemish-reducing composition is concentrated to 1.0 times the total weight of raw materials, and freeze-dried to obtain a whitening and blemish-reducing solid beverage.

3. Preparation of Whitening and Spotless Oral Liquid

The whitening composition is filled, sealed, sterilized, and packaged sequentially. When filling, control the filling temperature to ≥60°C; when sealing, control the sealing torque to 1.5Nm; The time is 30 minutes, and finally the oral liquid for whitening and blemishes is obtained.

Comparative example 1

Using the ratio of raw materials in Example 1, the whitening and lightening solid beverage is produced in batches by the enterprise.

In order to study the technical effect of the whitening and blemish-reducing product of the present invention, experiments on antioxidant performance, tyrosinase inhibition and anti-glycation were carried out on the whitening and blemish-reducing solid beverage.

Test Example 1 Antioxidation Experiment

1. Principle

Free radicals and active oxygen in skin tissue will be continuously produced in the process of cell metabolism. If they are not removed in time, they will affect the normal physiological process of the skin. In addition, ultraviolet radiation, environmental pollution and changes in nutritional status will form in the tissue. Oxidative stress causes changes in skin barrier function, abnormal pigment metabolism, and skin aging, manifested as lack of luster, deepened color, wrinkles, and premature aging. Natural substances with anti-oxidation and anti-tyrosinase activity can remove excess free radicals produced in tissues and cells, inhibit abnormal skin oxidative stress response through anti-oxidation, promote skin metabolism, inhibit pigment production, Increase skin elasticity and keep skin white and smooth.

2. Experimental method

2.1 Determination of DPPH free radical scavenging activity

Weigh 20 mg of DPPH, dissolve it in 95% ethanol, and dilute to 250 mL to prepare a 0.2 mmol/L DPPH solution. Prepare different concentrations of analytes, Vc, BHT solutions (0.2mg/ml, 0.4mg/ml, 0.8mg/ml, 1.2mg/ml, 1.6mg/ml, 2mg/ml), and dilute each sample with 0.2 Equal amounts (1ml) of mmol/L DPPH were mixed in a stoppered test tube, shaken well, reacted at room temperature in the dark for 60 minutes, and then measured the absorbance value at 517nm. Vc and BHT at the same concentration as the experimental samples were used as positive controls.

Free radical scavenging rate=( _AS -Ai)/As×100% (wherein As represents DPPH absorbance value, Ai represents DPPH absorbance value after adding test sample or antioxidant reference substance).

2.2 Determination of ABTS+ free radical scavenging ability

Prepare 7mmol/L ABTS aqueous solution, mix it with 2.45mmol/L potassium persulfate solution in equal volume, and place it in the dark at room temperature for 12h. The mixture was diluted with distilled water, and the absorbance measured at 734 nm was in the range of 0.7±0.03, which was used as ABTS+ for subsequent experiments. Mix 0.1ml of different concentrations of analyte, Vc, BHT solution (0.2mg/ml, 0.4mg/ml, 0.8mg/ml, 1.2mg/ml, 1.6mg/ml, 2mg/ml) with 0.9mL ABTS+, shake After a reaction at 30°C for 30 min, the absorbance was measured at 734 nm.

Free radical scavenging rate=(As-Ai)/As×100% (where As represents the absorbance value of ABTS, and Ai represents the absorbance value of ABTS after adding the sample or antioxidant).

2.3 Determination of antioxidant capacity index (ORAC)

Antioxidant capacity ORAC (also known as oxygen radical absorbance capacity, Oxygen Radical Absorbance Capacity) assay is a powerful tool for measuring the antioxidant capacity of biomolecules. Among many methods for evaluating antioxidant capacity, antioxidant capacity ORAC is recognized as a standard method for detecting antioxidant capacity, and is a standard evaluation method generally accepted at present.

During the experiment, after adding AAPH to start the fluorescence decay, the fluorescence changes in the reaction system of each sample were measured every two minutes until the fluorescence decay showed a baseline trend. The slower the fluorescence decay, the stronger the antioxidant capacity of the sample. By calculating the time required for each sample to resist fluorescence decay to half of the initial fluorescence intensity, the antioxidant capacity of each component of the formula and the antioxidant capacity of the two lyophilized powders of the finished product can be judged.

Solution preparation: 75mmol/l phosphate buffer solution, dilute 10 times with pure water for each experiment, and measure PH=7.4 with a pH meter; prepare fluorescein sodium concentrate with PBS, keep away from light and refrigerate, take 0.1ml with phosphoric acid when using Dilute the salt buffer solution to a 100ml volumetric flask, which is used as FL solution, and the final concentration is 63mmol/l; AAPH is ready-to-use and prepared with PBS, and the concentration is 12.8mmol/l; the sample solution is prepared with PBS, and the solid drink is freeze-dried The concentration of powder setting is 100μg/ml, 50μg/ml, 25μg/ml.

Reaction: The antioxidant capacity of the solid beverage freeze-dried powder produced in batches in the comparative example 1 and the laboratory solid beverage freeze-dried powder of Example 1 were measured respectively. Take 20 μl of the sample solution, add 20 μl PBS and 20 μl FL solution, and finally add 140 μl AAPH to start the reaction, measure the fluorescence intensity every 2 min at the excitation wavelength of 485 nm and emission wavelength of 538 nm until the fluorescence decay shows a baseline trend. Two groups of controls were set up: FL fluorescence natural attenuation control without AAPH added (AAPH-), and free radical action control (AAPH+) in the absence of antioxidants.

3 Experimental results

3.1 The scavenging ability of DPPH free radicals

Comparative Example 1 The whitening and lightening solid beverage (freeze-dried powder) produced in batch trial production was carried out with different concentration gradients (0.2, 0.4, 0.8, 1.2, 1.6, 2mg/ml), and the DPPH free radical scavenging experiment was carried out according to the above method.

The experimental results show that with the increase of the concentration, the ability of the whitening and lightening solid beverage to scavenge DPPH free radicals gradually increases. When the concentration reaches 2mg/ml, the free radical scavenging rate of the sample reaches (79.34±3.40)%, showing that the whitening and lightening solid beverage has a very significant ability to scavenge DPPH free radicals (Figure 1).

3.2ABTS+ free radical scavenging ability

Comparative Example 1 The whitening and lightening solid beverage (freeze-dried powder) produced in batch trial production was carried out with different concentration gradients (0.2, 0.4, 0.8, 1.2, 1.6, 2mg/ml), and the ABTS ⁺ free radical scavenging experiment was carried out according to the above method.

The experimental results show that with the increase of concentration, the ability of whitening and lightening solid beverage samples to scavenge ABTS ⁺ free radicals gradually increases. When the concentration reaches 2mg/ml, the free radical scavenging rate of the sample reaches (41.11±1.59)%, which shows that the whitening and lightening solid beverage has a certain ability to scavenge ABTS ⁺ free radicals (Figure 2).

3.3 Antioxidant Capacity Index (ORAC)

In the research on the antioxidant capacity of two kinds of whitening and blemish-lightening solid drink freeze-dried powders in Example 1 and Comparative Example 1, the two kinds of freeze-dried powders were formulated into solutions with three concentration gradients respectively, and there were 6 reaction systems in total. The antioxidant capacity of different concentrations of the same sample was tested separately.

The result shows that, in the three concentration reaction systems of embodiment 1 laboratory finished product whitening and blemish solid beverage freeze-dried powder and comparative example 1 batch trial production finished whitening and blemish solid beverage freeze-dried powder, when fluorescence intensity drops to half The time prolongs with the increase of the concentration, indicating that the antioxidant capacity of the two finished freeze-dried powders is enhanced with the increase of the concentration. The antioxidant capacity of the two lyophilized powder solutions of the same concentration was tested, and the results showed that there was no significant difference in the time required for the fluorescence intensity to drop to half in the reaction system of the two lyophilized powder solutions of the same concentration, indicating that the laboratory There was no significant difference (P>0.05) in the antioxidant capacity of the lyophilized powder and the batch trial-produced lyophilized powder (Figure 3, Table 1), indicating that the antioxidant capacity of the two finished lyophilized powders was consistent.

Table 1 The time required for the fluorescence intensity of the two kinds of freeze-dried powders of the finished product to decrease by half in the ORAC experiment

Test Example 2 Tyrosinase Inhibition Experiment

1. Experimental principle

Excessive deposition of melanin on the surface of the skin is the main cause of skin pigmentation, freckles, and chloasma. The tyrosinase present in the skin is an oxidase, which plays a key role in multiple reaction steps in the melanin synthesis process, and is the key enzyme and rate-limiting enzyme of melanin production. The natural substance for whitening and lightening spots of the present application can inhibit the production of melanin by inhibiting the activity of tyrosinase, and then play the role of lightening spots and whitening the skin.

2. Experimental method

2.1 Solution preparation

Prepare PBS solution (pH=6.8); Prepare 1mg/mL tyrosine solution and 200U/mL tyrosinase solution with PBS; 5mlPBS dilution).

The concentrations of the two experimental samples of the whitening and lightening solid beverage freeze-dried powder for the laboratory and the enterprise are set to 200mg/ml, 150mg/ml, 100mg/ml, 50mg/ml, and 25mg/ml respectively. β-arbutin was used as a positive control, and the concentration of β-arbutin was set to 2.0mg/ml, 1.5mg/ml, 1.0mg/ml, 0.5mg/ml, 0.25mg/ml.

2.2 Detection of tyrosinase activity

The reaction system was prepared according to Table 2, and placed in a water bath at 30°C for 30 minutes. 100 μL of each reaction tube was added to a 96-well plate, and the absorbance at 492 nm was measured with a microplate reader. The relative inhibition rate of the sample to tyrosinase was calculated according to the following formula.

In the formula, A ₁ is the absorbance of sample 1 in the reaction group; A ₂ is the absorbance of sample 2 without substrate in the reaction group; A _0-1 is the absorbance of reaction tube _0-1 without inhibitor; Substrate control absorbance.

Table 2 Tyrosinase inhibition reaction system

3. Experimental results

The tyrosinase activity inhibition experiment of β-arbutin and two freeze-dried powders of the finished product was carried out.

The results showed that the tyrosinase inhibition rates of the reference substance β-arbutin and the two freeze-dried powders of the finished product all showed a strengthening trend with the increase of the concentration (Fig. 4, 5). And when the concentration of the two kinds of freeze-dried powders of the finished product reaches 200mg/ml, the tyrosinase inhibition rate of the finished freeze-dried powder in the laboratory is (89.11 ± 4.51)%, and the tyrosinase inhibition rate of the finished freeze-dried powder of the enterprise is (93.57±4.33)%, indicating that the two freeze-dried powders of the finished product all have strong tyrosinase inhibitory activity. In addition, comparing the tyrosinase inhibition rates of the two lyophilized powders of various concentrations of finished products, no significant difference was found, indicating that the chemical resistance of the lyophilized powders of the two finished products is consistent.

Test Example 3 Anti-glycation experiment

1. Principle

Advanced glycation endproducts (AGEs) formed during protein non-enzymatic glycosylation are closely related to skin elasticity, color and other appearance indicators. Human skin tissue contains a large amount of collagen and elastin, which are distributed in the skin tissue in a fibrous form, forming the skeleton structure of the skin, playing an important supporting role in the skin tissue, and maintaining the strength and elasticity of the skin. has an irreplaceable role. When the glycation end products (AGEs) in the skin tissue increase, AGEs can form a cross-linking reaction with some proteins, deforming the support structure in the skin tissue, which not only affects its normal function, but also affects the adhesion of skin cells and cell growth process. In addition, the deposition of the complex formed by the cross-linking reaction between AGEs and the above-mentioned proteins in the cell matrix not only reduces the permeability of connective tissue, but also weakens the diffusion ability of nutrients and metabolic waste in the body, and increases the hardness of skin tissue, thus Decreases skin elasticity, eventually leading to skin aging and wrinkles.

In addition, in the case of factors such as ultraviolet radiation, AGEs present in skin tissue can induce skin oxidative stress, and oxidative stress can also increase the production of AGEs in skin tissue. Excessive AGEs lead to increased secretion of melanin, causing skin pigmentation.

Anti-glycation substances have an inhibitory effect on the excessive production of AGEs in skin tissue, thereby maintaining skin elasticity, delaying skin aging, and reducing wrinkles. At the same time, because the increase of AGEs is inhibited, the secretion of skin melanin is reduced, which can keep the skin fair and clear, and play the role of whitening and yellowing.

2. Experimental method

2.1 BSA-fructose model reaction system

A blank solution containing 10 mg/ml BSA and two lyophilized powders of Example 1 and Comparative Example 1 (concentrations of 0.25, 0.5, 1, and 2 mg/ml) was incubated at 37° C. for 7 days as a blank control. For the experimental groups, each reaction mixture contained 10 mg/ml BSA and 100 mM D-fructose (with or without sample solution).

All reaction mixtures were prepared in triplicate under sterile conditions and incubated at 37°C for 7 days. After incubation, perform intrinsic fluorescence measurement on a microplate reader with an excitation wavelength of 360 nm and an emission wavelength of 430 nm, (the characteristic fluorescence wavelength of BSA-derived AGEs).

Calculation: For all measured data, the background absorbance generated by the sample (with the corresponding concentration) was subtracted. All data were compared to a negative control containing only BSA and D-fructose, and the level of AGEs inhibition was calculated using the following formula:

Inhibition rate (%)=[1-(fluorescence intensity of treatment solution/fluorescence intensity of negative control solution)]×100

The positive control substance of the experiment was quercetin (50 μg/ml).

2.2 BSA-MGO model reaction system

A blank solution containing 10 mg/ml BSA and two lyophilized powders of Example 1 and Comparative Example 1 (concentrations of 0.25, 0.5, 1, and 2 mg/ml) was incubated at 37° C. for 7 days as a blank control. For the experimental groups, each reaction mixture contained 10 mg/ml BSA and 100 mM MGO (with or without sample solution).

All reaction mixtures were prepared in triplicate under sterile conditions and incubated at 37°C for 7 days. After incubation, perform intrinsic fluorescence measurement on a microplate reader with an excitation wavelength of 360 nm and an emission wavelength of 430 nm, (the characteristic fluorescence wavelength of BSA-derived AGEs).

Calculation: For all measured data, the background absorbance generated by the sample (with the corresponding concentration) was subtracted. All data were compared to negative controls containing only BSA and MGO, and the level of AGEs inhibition was calculated using the following formula:

Inhibition rate (%)=[1-(fluorescence intensity of treatment solution/fluorescence intensity of negative control solution)]×100

The positive control substance of the experiment was quercetin (50 μg/ml).

3. Experimental results

3.1 Anti-glycation effect of BSA-fructose reaction system

Whitening and blemish-relieving solid beverage finished freeze-dried powder (Example 1 and Comparative Example 1) were subjected to the same experiment according to the above-mentioned method.

The results showed that both the finished freeze-dried samples prepared by the laboratory and the enterprise showed good anti-glycation effect, and the anti-glycation effect tended to be significantly enhanced with the increase of the concentration gradient. When the concentrations of the two reached 2mg/ml respectively, the inhibition rates of AGEs formation in the freeze-dried samples of finished products prepared by the laboratory and those prepared by enterprises were (70.02 ± 0.06)% and (80.90 ± 0.08)% respectively, All showed significant inhibitory effects on the formation of AGEs, indicating that although there are differences in the inhibitory effects of the various components of the whitening and lightening solid beverage on the formation of AGEs, the finished products after the formulation have significantly enhanced inhibitory effects on the formation of AGEs, and the effect is much greater than that of each single component. The role of product components. At the same time, comparing the AGEs formation inhibition rate of the finished freeze-dried samples prepared by the laboratory and the finished freeze-dried samples prepared by the enterprise, there was no significant difference between the two, indicating that the formula, process and quality of the laboratory have strong stability (Figure 6 ).

3.2 Anti-glycation effect of BSA-MGO model reaction system

The finished lyophilized powder of whitening and blemish oral liquid (samples prepared by laboratories and enterprises) was subjected to the same anti-glycation experiment as above.

The results show that both the freeze-dried samples of the laboratory and the finished product of the enterprise have shown good anti-glycation effect, and the anti-glycation effect is obviously enhanced with the increase of the concentration gradient. When the concentrations of the two reached 2mg/ml respectively, the AGEs formation inhibition rates of the finished freeze-dried samples prepared by the laboratory and those prepared by the enterprise were (86.89±0.04)% and (89.75±0.01)%, respectively, both Shows significant inhibition of AGEs formation. The experimental results show that although there are differences in the inhibitory effects on the formation of AGEs in the BSA-MGO model reaction system of each component of the Whitening and Banban Oral Liquid, the effect of the finished product after formulation is significantly enhanced, and the effect is much greater than that of each single product component on the AGEs. Inhibition of AGEs formation. At the same time, comparing the AGEs formation inhibition rate of the finished product freeze-dried samples prepared by the laboratory and the freeze-dried samples of the finished products prepared by the enterprise, there is no significant difference in the effect of the two, indicating that the formula, process and quality of the laboratory have strong stability ( Figure 7).

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present invention, rather than limiting them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: It is still possible to modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the technical solutions of the various embodiments of the present invention. scope.

Claims (10)

1. A whitening composition that contains bird's nest peptide, is characterized in that, comprises the raw material of following weight fraction: Bird's nest peptide 0.1-0.3 parts, fish collagen peptide 4-6 parts, Cordyceps militaris 1.5-4 parts, burdock root 2.5-5 parts, Bao Le fruit powder 2-8 parts, seabuckthorn fruit powder 0.5-3 parts and stevia leaf 0.5- 3 copies. 2. The whitening and blemish-reducing composition according to claim 1, characterized in that the content of sialic acid in the bird's nest peptide is 10-11%. 3. The preparation method of the whitening and blemish-relieving composition according to any one of claims 1-2, characterized in that, comprising the following steps: S1. Separately pulverize Cordyceps militaris, burdock root and stevia leaves and decoct and extract them several times to obtain Cordyceps militaris extract, burdock root extract and stevia leaf extract; S2. Mixing the Cordyceps militaris extract, the burdock root extract, the stevia leaf extract and the remaining raw materials by weight to obtain a whitening and spot-reducing composition. 4. preparation method according to claim 3, is characterized in that, the method for described decoction extraction specifically comprises the following steps: Crush the raw materials to 40-60 mesh, add water to decoct and extract, filter the medicinal liquid after each decoction, keep the medicinal residues, and finally combine and filter the medicinal liquid obtained by each decoction to obtain the extract; When decocting each time, the amount of water used is 15-30 times the quality of the raw material, and each time decocting until the loss of 1/3-1/2 of the liquid medicine; The number of times of the decocting is 3-4 times. 5. A whitening and blemish-reducing solid drink, characterized in that it comprises the whitening and blemish-reducing composition according to any one of claims 1-2. 6. The preparation method of the whitening and blemish-reducing solid beverage according to claim 5, characterized in that the whitening and blemish-reducing composition is sequentially concentrated and freeze-dried to obtain the whitening and blemish-reducing solid beverage. 7. The preparation method according to claim 6, characterized in that, during the concentration, the whitening and blemish-reducing composition is concentrated to 0.9-1.1 times the total weight of raw materials. 8. An oral liquid for whitening and blemishes, characterized in that it comprises the composition for whitening and blemishes according to any one of claims 1-2. 9. The preparation method of the whitening and spot-lightening oral liquid according to claim 8, characterized in that the whitening and spot-lightening oral liquid is sequentially filled, sealed, sterilized and packaged to obtain the whitening and spot-lightening oral liquid. 10. The preparation method according to claim 9, characterized in that, during the filling, the filling temperature is controlled to be ≥60°C; When sealing, control the sealing torque to be 0.5-1.5Nm; During the sterilization, the temperature is controlled to be 105-115° C., and the time is 15-30 minutes.

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