CN115326716B - Immunodetection method and system - Google Patents
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- CN115326716B CN115326716B CN202211019708.2A CN202211019708A CN115326716B CN 115326716 B CN115326716 B CN 115326716B CN 202211019708 A CN202211019708 A CN 202211019708A CN 115326716 B CN115326716 B CN 115326716B
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- 238000000034 method Methods 0.000 title claims abstract description 21
- 239000002122 magnetic nanoparticle Substances 0.000 claims abstract description 39
- 238000003018 immunoassay Methods 0.000 claims abstract description 36
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 29
- 239000000243 solution Substances 0.000 claims abstract description 29
- 239000011259 mixed solution Substances 0.000 claims abstract description 26
- 230000005284 excitation Effects 0.000 claims abstract description 25
- 238000001514 detection method Methods 0.000 claims abstract description 23
- 239000007788 liquid Substances 0.000 claims description 14
- 230000003287 optical effect Effects 0.000 claims description 14
- 230000010287 polarization Effects 0.000 claims description 2
- 208000026278 immune system disease Diseases 0.000 claims 1
- 230000035945 sensitivity Effects 0.000 abstract description 6
- 239000002086 nanomaterial Substances 0.000 abstract description 2
- 230000000875 corresponding effect Effects 0.000 description 12
- 230000000694 effects Effects 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- 239000011553 magnetic fluid Substances 0.000 description 9
- 239000000427 antigen Substances 0.000 description 7
- 102000036639 antigens Human genes 0.000 description 7
- 108091007433 antigens Proteins 0.000 description 7
- 230000008859 change Effects 0.000 description 6
- 239000002245 particle Substances 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 230000005415 magnetization Effects 0.000 description 3
- 230000002596 correlated effect Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000008105 immune reaction Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000006249 magnetic particle Substances 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
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- 238000000576 coating method Methods 0.000 description 1
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- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000002096 quantum dot Substances 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000004154 testing of material Methods 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/21—Polarisation-affecting properties
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
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Abstract
The invention discloses an immunodetection method and an immunodetection system, and belongs to the field of nanomaterial testing. The method comprises the following steps: the incident light is reflected for a plurality of times in the functional magnetic nano particle immunoassay reagent under the excitation of the alternating-current magnetic field; converting the emergent light after multiple reflections into a voltage signal, and extracting a magneto-optical signal corresponding to the immunoassay reagent; mixing an immunoassay reagent with a solution to be tested, and enabling incident light to be reflected for multiple times in the mixed solution under the excitation of an alternating-current magnetic field; converting the emergent light after multiple reflections into a voltage signal, and extracting a magneto-optical signal corresponding to the mixed solution; and when the difference degree of the magneto-optical signals corresponding to the immunoassay reagent and the magneto-optical signals corresponding to the mixed solution exceeds a preset threshold value, judging that the solution to be detected contains target molecules to be detected. The invention can improve the lower limit of the detection concentration of the molecules to be detected and improve the precision and sensitivity of the immune detection; the method has the advantage of no-wash convenience, and is simpler to operate than the existing magneto-optical immunodetection method.
Description
Technical Field
The invention belongs to the technical field of nano material testing, and particularly relates to an immune detection method and system.
Background
Immunoassays use a variety of labels to determine antibodies or antigens. The most common methods are enzyme-linked immunosorbent assays, radioimmunoassays and real-time law polymerase chain reaction. Recently, magnetic immunoassays have received attention. The magnetic nanoparticles can be used as a label of a detected object in immunodetection due to the unique magnetic properties. The magnetic properties of the magnetic nanoparticles can be used to characterize the detection characteristics of the molecules to be detected that interact with them. The magnetic related quantities currently being widely studied are magnetic relaxation, remanent magnetization, ac susceptibility, saturation magnetization, brownian relaxation time, etc. The specific functional group is utilized in the solution to bind the molecule to be detected and the magnetic nano particle together, so that the magnetic information of the magnetic nano particle can be changed. The change in the magnetization response of the magnetic nanoparticles measured by the sensor is related to the concentration of the molecules to be measured.
The optical measurement method has the outstanding advantages of non-contact, high sensitivity, high precision and the like. The magneto-optical effect is a new method that can measure the magnetic information of magnetic nanoparticles. The magnetic field is applied to the magnetic fluid, and the magnetic nanoparticles are aggregated into chain-shaped clusters along the direction of the magnetic field, so that the anisotropic property is shown. Light propagates in the magnetized magnetic fluid, which produces a series of magneto-optical effects that result in a change in the magneto-optical response signal. The intensity of the magneto-optical response signal is related to the concentration information of the magnetic fluid, so that the immunodetection can be realized by detecting the intensity of the magneto-optical effect.
The magneto-optical immunodetection method commonly used at present comprises a magnetic fluorescent nano probe detection method and a magnetic particle chemiluminescence immunoassay method. The unbound fluorescent quantum dots in the magnetic fluorescent nano probe detection can generate certain fluorescent intensity to interfere with the detection of the conjugate, and magnetic field separation is needed. The magnetic particle chemiluminescent immunoassay requires washing after the antigen-antibody reaction is completed to remove substances not bound to the immune complex. The washing process will reduce the amount of the labeled analyte, resulting in an increase in the detection limit.
Disclosure of Invention
In order to meet the above defects or improvement demands of the prior art, the invention provides an immunodetection method and an immunodetection system, which aim to improve the concentration lower limit of molecules to be detected and realize magneto-optical immunodetection with higher precision and sensitivity.
To achieve the above object, according to one aspect of the present invention, there is provided an immunoassay method comprising:
S1, enabling incident light to be reflected for multiple times in a functional magnetic nanoparticle immunoassay reagent under the excitation of an alternating-current magnetic field;
S2, converting the emergent light after multiple reflections into a voltage signal, and extracting a magneto-optical signal corresponding to the immunoassay reagent;
s3, mixing an immunoassay reagent with the solution to be tested, and enabling incident light to be reflected for multiple times in the mixed solution under the excitation of an alternating-current magnetic field; the incident light and the alternating current magnetic field are the same as those in the step S1;
S4, converting the emergent light after multiple reflections into a voltage signal, and extracting a magneto-optical signal corresponding to the mixed solution;
s5, when the difference degree of the magneto-optical signals corresponding to the immunoassay reagent and the magneto-optical signals corresponding to the mixed solution exceeds a preset threshold, judging that the solution to be detected contains target molecules to be detected.
Further, the magneto-optical signal is a ratio of a second harmonic component to a direct current component in the voltage signal.
Further, the method includes forming horizontally polarized light from the incident light through the polarizer.
Further, the alternating excitation magnetic field is perpendicular to the propagation direction of the incident light.
Further, the incident light is a laser light having a wavelength of 520 nm.
According to another aspect of the present invention there is provided an immunoassay system comprising: the device comprises a laser, a polaroid, a coil, an optical liquid cavity, a photoelectric detector and a computer;
a laser for generating incident light;
A polarizing plate for changing a polarization direction of incident light;
A coil for generating an alternating excitation magnetic field;
the optical liquid cavity is used for containing a functional magnetic nano particle immunoassay reagent or a mixed solution of the immunoassay reagent and a solution to be tested, so that incident light is reflected for multiple times in the functional magnetic nano particle immunoassay reagent or the mixed solution under the excitation of an alternating-current magnetic field; the structure of the optical liquid cavity is a cube container with reflecting mirrors plated on the front and back surfaces;
The photoelectric detector is used for converting the emergent light after multiple reflections into a voltage signal;
The computer is used for extracting magneto-optical signals corresponding to the immunoassay reagent and the mixed solution; and when the difference degree of the magneto-optical signals corresponding to the immunoassay reagent and the magneto-optical signals corresponding to the mixed solution exceeds a preset threshold value, judging that the solution to be detected contains target molecules to be detected.
Further, the alternating excitation magnetic field generated by the coil is perpendicular to the propagation direction of the incident light generated by the laser.
In general, the above technical solution conceived by the present invention can achieve the following advantageous effects compared to the prior art.
(1) Compared with the alternating current sensor of the traditional detection scheme, the magneto-optical detection system can detect the concentration of the magnetic nanoparticles at least three orders of magnitude less in a short time, and the magneto-optical detection system can reflect light in magnetic fluid for multiple times on the basis of the existing magneto-optical effect, so that the optical path is increased, the magneto-optical signal intensity can be greatly improved, the lower limit of the detection concentration of molecules to be detected can be further improved, and the precision and sensitivity of immunodetection are improved.
(2) The magneto-optical signal is represented by the ratio of the amplitude of the second harmonic to the direct current component, and the experimental result shows that the second harmonic generated by pure water under the excitation of an alternating current magnetic field is negligible, so that the magneto-optical signal adopted by the method is irrelevant to the diamagnetism of the water, the interference of the water on the detection result can be ignored, and the mixed solution has the convenience of no washing; because the unbound object to be detected has no influence on the size of the magneto-optical signal, the mixed solution does not need to be cleaned after the antigen-antibody immunoreacts, and the detection can be directly carried out, and compared with the existing magneto-optical immunodetection method, the operation is simple.
Drawings
FIG. 1 is a diagram of a magneto-optical immunodetection apparatus of the present invention;
FIG. 2 is a graph showing magneto-optical signals of magnetic nanoparticle solutions of different concentrations as a function of magnetic field;
FIG. 3 is a graph showing the change of magneto-optical signal with magnetic field at different reflection times;
Fig. 4 is a graph showing magneto-optical signals of magnetic nanoparticles of different particle diameters as a function of magnetic field.
Detailed Description
The present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention. In addition, the technical features of the embodiments of the present invention described below may be combined with each other as long as they do not collide with each other.
In order to solve the technical problems of low efficiency and low detection sensitivity of the traditional immunodetection method based on magneto-optical effect, the invention provides an immunodetection method based on an optical liquid cavity, which has the following overall thought: the ratio of the second harmonic amplitude of the magneto-optical response signal to the direct current component is improved by utilizing the repeated reflection of light in the optical liquid cavity, so that the concentration lower limit in immunodetection is reduced. Comparing the signals of the immunoassay reagent and the mixed solution, and if the difference value of the signals exceeds a threshold value, judging that the solution to be detected contains the target molecule. Due to the diamagnetism of water, certain harmonic interference can be generated when alternating current excitation is applied to the magnetic nanoparticle solution. However, the experimental result shows that the second harmonic wave generated by pure water under the excitation of an alternating magnetic field is negligible, so that the magneto-optical signal adopted by the method is irrelevant to the diamagnetism of water, the interference of water on the detection result can be ignored, and the mixed solution has the convenience of no-washing, so that the operation is simpler; the magnetic signal is detected by utilizing the light measurement technology, so that the detection sensitivity can be improved, and the rapid detection can be realized.
The method specifically comprises the following steps:
S1, coating magnetic nano particles with an antibody (antigen) of an object to be detected to form a magnetic nano particle immune complex, and then configuring a functional magnetic nano particle immune assay reagent. The incident light is reflected for a plurality of times in the functional magnetic nano particle immunoassay reagent under the excitation of the alternating-current magnetic field;
Specifically, a laser having a wavelength of 520nm at which the absorbance of light by water is small is used to generate laser light for detecting the concentration of the liquid. The wavelength of the magneto-optical signal with smaller water absorptivity and larger magneto-optical signal can be comprehensively considered, so that the loss caused by absorption when light passes through the magnetic fluid is reduced, and the magneto-optical signal is enhanced.
The magnetic nano particles under the action of the magnetic field show anisotropy, and the absorption coefficient and the scattering coefficient can be changed after light passes through the magnetic nano particle solution, so that the transmissivity of incident light is changed. The attenuation of light in conventional solutions satisfies the linear law of absorption, namely lambert's (j.h.lambert) law:
I＝I0exp(-αL)
Wherein, I 0 and I are respectively the incident light intensity and the emergent light intensity, L is the path of light passing through the solution, and alpha is the attenuation coefficient. According to the magneto-optical signal obtained through experiments in the invention, the absorption law is corrected, and the relationship between the emergent light intensity and the incident light intensity is obtained as follows:
I＝I0exp[-α(H)L]
wherein H is the excitation magnetic field. According to Taylor formula, there are
From the above formula, when the applied excitation magnetic field is sinusoidal ac field h=h 0 sin ωt, the outgoing optical signal contains various subharmonics, and changing parameters such as concentration of the magnetic nanoparticle solution and light intensity of the incident light can change the corresponding harmonic amplitude. The second harmonic is adopted in the invention because the amplitude of the second harmonic is the largest among the subharmonics, and the harmonic component is positively correlated with the path of light passing through the magnetic nanoparticle solution under the action of the magnetic field. The ratio of the second harmonic to the direct current component is used as a final magneto-optical signal, so that the difference of detection results caused by different incident light intensities can be eliminated.
In order to distinguish the magneto-optical effect generated by the transmittance change under the action of the magnetic field mentioned in the present invention from the conventional magneto-optical birefringence effect and magneto-optical faraday effect, the length of the liquid container used will be described. Magneto-optical signals obtained by magneto-optical birefringence effect are derived from phase differencesThe magneto-optical signal obtained by magneto-optical Faraday is derived from the rotation angle θ byAnd sine or cosine values of θ to calculate the phase difference and rotation angle.And the proportional relationship of θ to the path of light through the magnetic fluid is applicable in the range of 0 to pi/2, and thus the thickness of the liquid container is limited to the order of μm. The single-pass path of the optical liquid cavity in the invention is in the order of mm, so that the optical liquid cavity is not suitable for the traditional magneto-optical birefringence effect and magneto-optical Faraday effect, and the explanation is made through the change of the transmissivity.
When the horizontal polarized light is repeatedly reflected in the magnetic fluid under the excitation of the alternating-current magnetic field, the distance of the light passing through the magnetic fluid increases along with the increase of the reflection times, and the magneto-optical signal is positively correlated with the distance of the light passing through the magnetic fluid, so that the magneto-optical signal can be greatly improved. The distance of light passing through can also be increased through the mode that increases the cell thickness, but the volume of cell and the volume of consumed solution all can increase, and the cost improves, and is unfavorable for miniaturization and integration. Preferably, the alternating excitation magnetic field is perpendicular to the propagation direction of the incident light, and a stronger signal can be obtained.
S2, converting the emergent light after multiple reflections into a voltage signal, and extracting a magneto-optical signal corresponding to the immunoassay reagent;
the direct current component V 0 and the second harmonic amplitude V 2f are obtained through processing of the voltage signals, wherein f represents the excitation frequency of the alternating current magnetic field. The magneto-optical signal s=v 2f/V0 used for analysis can eliminate the influence caused by the different light intensities of the incident light. The magneto-optical signals mentioned in the invention all refer to the ratio of the amplitude of the second harmonic in the photovoltage to the direct current component.
S3, mixing an immunoassay reagent with an antigen (antibody) to be detected, and forming an antigen-antibody-magnetic nanoparticle conjugate after immune reaction, so that incident light is reflected for multiple times in a mixed solution under the excitation of an alternating-current magnetic field; the incident light and the alternating current magnetic field are the same as those in the step S1;
S4, converting the emergent light after multiple reflections into a voltage signal, and extracting a magneto-optical signal corresponding to the mixed solution;
S5, the magneto-optical signal of the immunological identification reagent is only related to the magnetic nano-particles, and the magneto-optical signal of the mixed solution contains the total characteristic information of the unbound magnetic nano-particles and the conjugate which generates immune reaction with the antigen (antibody) to be detected. The magneto-optical signal is related to the total particle size of the magnetic nanoparticles and their conjugates. Therefore, when the difference degree of the magneto-optical signal corresponding to the immunoassay reagent and the magneto-optical signal corresponding to the mixed solution exceeds the preset threshold value, the solution to be detected is judged to contain the target molecule to be detected
If the difference between the magneto-optical signal s 1 of the functional magnetic nanoparticle solution and the magneto-optical signal s 2 of the mixed solution exceeds a threshold value, the solution to be detected on the surface contains target molecules. The specific antibody (antigen) on the surface of the functional magnetic nano particle is changed, so that the detection of different kinds of antigens (antibodies) can be realized
The magneto-optical signal is only related to the particles containing the magnetic nano particles, and the unbound antigen (antibody) to be detected does not influence the detection result, so that the immunodetection method provided by the invention is free from cleaning.
Example one:
Magneto-optical signals of different concentrations of magnetic nanoparticle solutions were compared. As shown in FIG. 2, the magnetic nanoparticle solution concentration is 100 μg/ml, 10 μg/ml, 1 μg/ml and distilled water, respectively, and the magneto-optical signal is changed along with the amplitude of the AC excitation magnetic field. It can be seen that when the alternating excitation field is the same in magnitude, the magneto-optical signal increases with increasing concentration of the magnetic nanoparticle solution.
Example two:
Magneto-optical signals of different reflection times are compared. As shown in fig. 3, n is the number of times light passes through the magnetic nanoparticle solution, and the relationship between n and the number of reflections m is: n=2m+1. It can be seen that as the number of reflections increases, so does the magneto-optical signal.
Example three:
Magneto-optical signals of magnetic nanoparticles of the same volume fraction and different particle diameters were compared. As shown in FIG. 4, the experimental results of the magnetic nanoparticles having particle diameters of 30nm, 20nm and 10nm, respectively, were compared with distilled water. It can be seen that as the particle size increases, the magneto-optical signal also increases.
Therefore, the magneto-optical immunodetection method based on the optical liquid cavity can indeed improve the intensity of magneto-optical signals on the basis of magneto-optical immunodetection, and improves the lower concentration limit of detection.
It will be readily appreciated by those skilled in the art that the foregoing description is merely a preferred embodiment of the invention and is not intended to limit the invention, but any modifications, equivalents, improvements or alternatives falling within the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (6)
1. An immunoassay method for the detection of an immune disorder, characterized by comprising the following steps:
S1, enabling incident light to be reflected for multiple times in a functional magnetic nanoparticle immunoassay reagent under the excitation of an alternating-current magnetic field;
S2, converting the emergent light after multiple reflections into a voltage signal, and extracting a magneto-optical signal corresponding to the immunoassay reagent;
s3, mixing an immunoassay reagent with the solution to be tested, and enabling incident light to be reflected for multiple times in the mixed solution under the excitation of an alternating-current magnetic field; the incident light and the alternating current magnetic field are the same as those in the step S1;
S4, converting the emergent light after multiple reflections into a voltage signal, and extracting a magneto-optical signal corresponding to the mixed solution;
S5, when the difference degree of the magneto-optical signal corresponding to the immunoassay reagent and the magneto-optical signal corresponding to the mixed solution exceeds a preset threshold, determining that the solution to be detected contains target molecules to be detected, wherein the magneto-optical signal is the ratio of a second harmonic component to a direct current component in the voltage signal.
2. The method of claim 1, further comprising passing the incident light through a polarizer to form polarized light in a horizontal direction.
3. An immunoassay according to claim 1 or 2, wherein the alternating magnetic field is perpendicular to the direction of propagation of the incident light.
4. The method of claim 1, wherein the incident light is a laser light having a wavelength of 520 nm.
5. An immunoassay system, comprising: the device comprises a laser, a polaroid, a coil, an optical liquid cavity, a photoelectric detector and a computer;
a laser for generating incident light;
A polarizing plate for changing a polarization direction of incident light;
A coil for generating an alternating excitation magnetic field;
the optical liquid cavity is used for containing a functional magnetic nano particle immunoassay reagent or a mixed solution of the immunoassay reagent and a solution to be tested, so that incident light is reflected for multiple times in the functional magnetic nano particle immunoassay reagent or the mixed solution under the excitation of an alternating-current magnetic field; the structure of the optical liquid cavity is a cube container with reflecting mirrors plated on the front and back surfaces;
The photoelectric detector is used for converting the emergent light after multiple reflections into a voltage signal;
the computer is used for extracting magneto-optical signals corresponding to the immunoassay reagent and the mixed solution; when the difference degree of the magneto-optical signal corresponding to the immunoassay reagent and the magneto-optical signal corresponding to the mixed solution exceeds a preset threshold, determining that the solution to be detected contains target molecules to be detected, wherein the magneto-optical signal is the ratio of a second harmonic component to a direct current component in a voltage signal.
6. The immunodetection system of claim 5, wherein the alternating excitation magnetic field generated by the coil is perpendicular to the direction of propagation of the incident light generated by the laser.
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| CN202211019708.2A CN115326716B (en) | 2022-08-24 | 2022-08-24 | Immunodetection method and system |
| PCT/CN2022/138911 WO2024040802A1 (en) | 2022-08-24 | 2022-12-14 | Immunoasssay method and system |
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| JPS62118255A (en) * | 1985-11-19 | 1987-05-29 | Toshimitsu Musha | Detection of immunological reaction by using magnetic field |
| US5238811A (en) * | 1988-04-26 | 1993-08-24 | Nippon Telegraph And Telephone Corporation | Laser magnetic immunoassay method and apparatus therefor and superparamagnetic material-labeled body and method for the manufacture of same |
| CN1184482C (en) * | 2002-12-30 | 2005-01-12 | 上海交通大学 | Magnetic separated immunoreaction optical inspecting device and method |
| US7639359B2 (en) * | 2006-10-23 | 2009-12-29 | UChicagoArgonne, LLC | Magneto-optic biosensor using bio-functionalized magnetized nanoparticles |
| US9874564B2 (en) * | 2011-03-22 | 2018-01-23 | The General Hospital Corporation | Detection of targets using magnetic resonance |
| CN106596473A (en) * | 2016-10-27 | 2017-04-26 | 中国科学院电子学研究所 | Optic detector based on electric control magnet enrichment-separation and total internal reflection magnetic imaging |
| US11313793B2 (en) * | 2017-12-15 | 2022-04-26 | Politecnico Di Milano | Opto-magnetic sensor device and molecular recognition system |
| US10161856B1 (en) * | 2018-01-19 | 2018-12-25 | Ping-Chieh Wu | Magneto-optical bio-detection devices having high sensitivity |
| CN115326716B (en) * | 2022-08-24 | 2024-11-19 | 华中科技大学 | Immunodetection method and system |
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