CN115283036A

CN115283036A - Variable interval adapter device applicable to eight-row pipettor

Info

Publication number: CN115283036A
Application number: CN202211219206.4A
Authority: CN
Inventors: 阔瀛; 朱小辉; 闫丽盈; 乔杰
Original assignee: Peking University Third Hospital Peking University Third Clinical Medical College
Current assignee: Peking University Third Hospital Peking University Third Clinical Medical College
Priority date: 2022-10-08
Filing date: 2022-10-08
Publication date: 2022-11-04

Abstract

The invention relates to a variable-interval adapter device applicable to an eight-row pipette, which comprises an adapter shell, a top adapter head, a hose, a slide rail and a bottom adapter head, wherein the top adapter head is fixedly arranged at the top of the adapter shell, the slide rail is fixedly arranged at the bottom of the adapter shell, and the hose is connected between the top adapter head and the bottom adapter head; the bottom adapter can be slidably arranged on the slide rail; the top adapter is used for being in adaptive connection with the conventional 0.5-10uL eight-row pipette tip; the bottom adapter is used for being in adaptive connection with the pipette head; the bottom adapter is provided with a plurality of bottom adapters, and the distance between the adjacent bottom adapters is adjusted by enabling the bottom adapters to slide on the sliding rails.

Description

Variable interval adapter device applicable to eight-row pipettor

Technical Field

The invention relates to the technical field of experimental instruments, in particular to a variable-interval adapting device applicable to an eight-gang-row liquid shifter.

Background

In the process of preparing high-throughput sequencing libraries, after the preparation of a single sample library is finished, conventionally, the concentration of each single sample library is obtained (single sample library quantification), and then a certain volume of the single sample library is absorbed according to the requirement of the mixed library to perform mixed library and subsequent pulling on-machine sequencing (table 1).

TABLE 1

Currently, most of the high-throughput library preparation operations are directly stored in 0.2mL eight-row tubes when the single-sample library preparation is completed, while the conventional library quantification operation needs to be completed in a 0.6mL high-transmittance centrifuge tube. This means that the operator needs to pipette 1 μ L of the single sample library one by one from 0.2mL of eight-row tube into a 0.6mL high transmittance centrifuge tube containing the quantification buffer, and then put the library into the quantification apparatus to quantify the single library sample. Such pipetting is not laborious to carry out with a small sample size. And once the sample size is large, such as: some laboratories build libraries at one time, the number of single sample libraries is generally more than or equal to 64, which means that the operator is hard to repeat the pipetting operation for 64 times.

Therefore, the applicant designs a variable-pitch adapter device suitable for a conventional 0.5-10 microliter eight-row pipette, so as to directly pipette liquid from 0.2mL eight-row tubes into a 0.6mL high-transmittance centrifuge tube at one time. In the design measurement process, the pipette device can simultaneously realize the pipetting into a 1.5mL centrifuge tube under the condition of satisfying the pipetting into a 0.6mL centrifuge tube when using a laboratory conventional centrifuge tube rack.

Disclosure of Invention

The invention aims to provide a variable-spacing adapting device applicable to a conventional 0.5-10 microliter eight-row liquid transfer device, and the technical problem to be solved at least comprises the requirement of directly transferring liquid from eight-row tubes to high-transmittance centrifugal tubes with different capacities at one time.

In order to achieve the purpose, the invention provides a variable-interval adapter device applicable to an eight-row pipette, which comprises an adapter shell, a top adapter head, a hose, a slide rail and a bottom adapter head, wherein the top adapter head is fixedly arranged at the top of the adapter shell, the slide rail is fixedly arranged at the bottom of the adapter shell, and the hose is connected between the top adapter head and the bottom adapter head; the bottom adapter can be slidably arranged on the slide rail; the top adapter is used for being in adaptive connection with the conventional 0.5-10uL eight-row pipette tip; the bottom adapter is used for being in adaptive connection with the pipette head; the bottom adapter is provided with a plurality of bottom adapters, and the distance between the adjacent bottom adapters is adjusted by enabling the bottom adapters to slide on the sliding rail.

Preferably, the adapter housing comprises an upper surface disposed at the top and a lower surface disposed at the bottom, the top adapter is fixedly disposed on the upper surface of the adapter housing, and the slide rail is fixedly disposed on the lower surface of the adapter housing.

Preferably, the number of the top adapter heads is equal to that of the bottom adapter heads.

Preferably, the number of the hoses is multiple, and each corresponding top adapter and bottom adapter are connected through one hose.

Preferably, the connection between the hose and the top adapter and the bottom adapter is airtight.

Preferably, the bottom adapter is slidably disposed on the slide rail through a slot.

Preferably, the top adapter head comprises a first adapter part and a second adapter part, the interiors of the first adapter part and the second adapter part are communicated, and the first adapter part is used for being in fit connection with a conventional 0.5-10uL eight-row pipette tip; the second adapting part is used for being connected with the hose in a sealing mode.

Preferably, the slide rail includes a first rail and a second rail, and the first rail and the second rail are disposed in parallel with each other.

Preferably, the bottom adapter comprises an upper adapter part and a lower adapter part, the interiors of the upper adapter part and the lower adapter part are communicated, and the upper adapter part is used for being connected with the hose in a sealing manner; the lower adapting part is used for being in adaptive connection with the pipette head.

Preferably, the clamping groove comprises a first clamping block and a second clamping block, an arc-shaped clamping portion is arranged at the butt joint of the first clamping block and the second clamping block, the first clamping block and the second clamping block clamp the upper adapting portion of the bottom adapting head in the arc-shaped clamping portion, and the bottom adapting head is connected with the clamping groove.

Advantageous effects

Compared with the prior art, the invention has the beneficial effects that:

the variable-spacing adapting device applicable to the eight-row pipette can directly pipette liquid from 0.2mL of eight-row tubes into a 0.6mL high-transmittance centrifugal tube at one time. In the design and measurement process, the conventional centrifuge tube rack in a laboratory is used, and the pipette capacity of the centrifuge tube with the volume of 1.5mL can be simultaneously satisfied under the condition that the pipette capacity of the centrifuge tube with the volume of 0.6mL is satisfied.

A conventional 0.5-10uL eight-row pipette is carried with the variable-interval adapter device designed by the application, and after a conventional 10uL gun head is inserted in an original shrinkage state, liquid suction from a 0.2mL eight-row centrifuge tube is completed, the interval is adjusted in a sliding manner, the operation of spitting liquid into a 0.6mL or 1.5mL centrifuge tube is completed, and the workload of operators is greatly reduced.

Drawings

The accompanying drawings are included to provide a further understanding of the present invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention.

Fig. 1 is a schematic diagram of a centrifuge tube rack conventionally used in a laboratory.

Fig. 2 is a schematic structural diagram of a conventional 0.5-10ul manual eight-gang pipette.

Fig. 3 is a schematic structural view of the variable pitch adapter in a first state.

Fig. 4 is a schematic structural view of the variable pitch adapter in a second state.

Fig. 5 is a schematic structural view of the variable pitch adapter in a third state.

Fig. 6 is a schematic diagram of a state change of the variable pitch adapter.

Fig. 7 is a schematic diagram of the state change of the bottom adapter moving to the left by different distances.

Fig. 8 is a schematic structural view of a conventional 0.5-10uL eight-gang pipette tip.

Fig. 9 is a schematic assembly of a conventional 0.5-10uL octal gang pipette tip and pipette tip.

Fig. 10 is a schematic structural view of the top adapter.

Fig. 11 is a simulation schematic of the assembly of a conventional 0.5-10uL eight gang pipette tip with a top adapter.

Fig. 12 is a schematic view of the arrangement design of the top adapter.

Fig. 13 is a front view of the variable pitch adapter.

Fig. 14 is a schematic structural view of the bottom adapter.

Fig. 15 is a schematic view of the assembly of the bottom adapter head with a pipette tip.

Fig. 16 is a front view, a bottom view and a partially enlarged schematic view of the variable pitch adapter.

Fig. 17 is a front view and a bottom view of the variable pitch adapter shown in fig. 16 after pitch adjustment is performed.

Fig. 18 is a top view of the card slot.

Fig. 19 is a side view of the card slot.

Fig. 20 is a top view of the slide rail.

Detailed Description

The present invention is described in more detail below to facilitate an understanding of the present invention.

As shown in FIG. 1 and FIG. 2, the centrifuge tube rack conventionally used in laboratories comprises 0.5/0.6mL centrifuge tubes and 1.5mL centrifuge tubes, the corresponding opening distance L of the 0.5/0.6mL centrifuge tubes is 7.88 + -0.01 mm, the aperture R is 8.21 + -0.05 mm, and the distance M required to be satisfied between tip heads during pipetting is 16.10 + -0.05 mm; the corresponding opening distance L of a 1.5mL centrifuge tube is 4.58 +/-0.05 mm, the aperture R is 11.49 +/-0.05 mm, and the distance M required to be met between tip heads during liquid transfer is 16.10 +/-0.05 mm.

A conventional 0.5-10uL eight-row pipette is carried with the variable-interval adapter device designed by the application, and after a conventional 10uL pipette head is inserted in an original shrinkage state, liquid suction from a 0.2mL eight-row centrifuge tube is completed, the interval is adjusted in a sliding manner, and the operation of spitting liquid into a 0.6mL or 1.5mL centrifuge tube is completed.

As shown in fig. 3 to 20, the variable-pitch adapter device for an eight-gang pipette according to the present invention includes an adapter housing 1, a top adapter 2, a hose 3, a slide rail 4 and a bottom adapter 5, wherein the top adapter 2 is fixedly disposed on the top of the adapter housing 1, the slide rail 4 is fixedly disposed on the bottom of the adapter housing 1, and the hose 3 is connected between the top adapter 2 and the bottom adapter 5; the bottom adapter 5 is slidably arranged on the slide rail 4; the top adapter 2 is used for being in adaptive connection with a conventional 0.5-10uL eight-gang liquid transfer device gun tip 7; the bottom adapter 5 is used for being in adaptive connection with the pipette tip 8; the bottom adapter 5 is provided with a plurality of bottom adapters, and the distance between the adjacent bottom adapters 5 is adjusted by sliding the bottom adapter 5 on the sliding rail 4.

Preferably, the adapter housing 1 comprises an upper surface disposed at the top and a lower surface disposed at the bottom, the top adapter 2 is fixedly disposed on the upper surface of the adapter housing 1, and the sliding rail 4 is fixedly disposed on the lower surface of the adapter housing 1.

Preferably, the number of the top adapter 2 is equal to the number of the bottom adapter 5, and the number of the top adapter 2 is equal to the number of the bottom adapter 2.

Preferably, the number of the hoses 3 is multiple, and each corresponding top adapter 2 and bottom adapter 5 are connected through one hose 3.

Preferably, the connection between the hose 3 and the top adapter 2 and the bottom adapter 5 is airtight.

Preferably, the bottom adapter 5 is slidably disposed on the slide rail 4 through a slot 6.

Preferably, the top adapter 2 comprises a first adapter 21 and a second adapter 22, the interiors of the first adapter 21 and the second adapter 22 are communicated, and the first adapter 21 is used for being matched and connected with a conventional 0.5-10uL eight-row pipette tip 7; the second adapter part 22 is used for sealing connection with the hose 3.

Preferably, the sliding rail 4 comprises a first rail 41 and a second rail 42, and the first rail 41 and the second rail 42 are arranged in parallel.

Preferably, the bottom adapter 5 comprises an upper adapter part 51 and a lower adapter part 52, the interiors of the upper adapter part 51 and the lower adapter part 52 are communicated, and the upper adapter part 51 is used for being connected with the hose 3 in a sealing way; the lower adapting part 52 is used for being adaptively connected with the pipette tip 8.

Preferably, the clamping groove 6 includes a first clamping block 61 and a second clamping block 62, an arc-shaped clamping portion is disposed at a butt joint of the first clamping block 61 and the second clamping block 62, and the first clamping block 61 and the second clamping block 62 clamp the upper adapting portion 51 of the bottom adapting head 5 in the arc-shaped clamping portion, so as to connect the bottom adapting head 5 and the clamping groove 6.

As shown in fig. 5, if the distance H to be satisfied between tip heads (i.e. pipette tips 8) during pipetting is 16.10 ± 0.05mm, the distance between adjacent bottom adapter heads 5 needs to be adjusted by sliding the bottom adapter heads 5 on the slide rail 4. As shown in FIG. 7, one of the bottom adapter heads is shown to be moved 4.0mm, 5.08mm, 16.1mm and 22.116mm to the left, respectively.

In the variable-pitch adapting device applicable to the eight-gang pipette, preferably, through holes are formed in the first fixture block 61 and the second fixture block 62, and the first rail 41 and the second rail 42 are inserted into the through holes, so that the clamping groove 6 is slidably arranged on the slide rail 4.

In the variable-interval adapting device applicable to the eight-row liquid transfer device, the gun tip 7 of the conventional 0.5-10uL eight-row liquid transfer device needs to be ensured to be tightly inserted into the first adapting part 21 of the top adapting head 2; the lower adapter part 52 of the bottom adapter 5 can be tightly inserted into the pipette tip 8 while ensuring that the upper adapter part 51 is used for air tightness with the hose 3 and the second adapter part 22 is used for air tightness with the hose 3.

Preferably, the first block 61 and the second block 62 can be connected by bolts, and the upper adapting portion 51 of the bottom adapting head 5 is clamped in the arc-shaped clamping portion by tightening the bolts, so that the bottom adapting head 5 is connected with the clamping groove 6.

The foregoing describes preferred embodiments of the present invention, but is not intended to limit the invention thereto. Modifications and variations of the embodiments disclosed herein may be made by those skilled in the art without departing from the scope and spirit of the invention.

Claims

1. The variable-interval adapting device suitable for the eight-row liquid transfer device is characterized by comprising an adapter shell, a top adapting head, a hose, a sliding rail and a bottom adapting head, wherein the top adapting head is fixedly arranged at the top of the adapter shell, the sliding rail is fixedly arranged at the bottom of the adapter shell, and the hose is connected between the top adapting head and the bottom adapting head; the bottom adapter can be slidably arranged on the slide rail; the top adapter is used for being in adaptive connection with the conventional 0.5-10uL eight-row pipette tip; the bottom adapter is used for being in adaptive connection with the pipette head; the bottom adapter is provided with a plurality of bottom adapters, and the distance between the adjacent bottom adapters is adjusted by enabling the bottom adapters to slide on the sliding rails.

2. The device according to claim 1, wherein the adapter housing comprises an upper surface disposed at the top and a lower surface disposed at the bottom, the top adapter is fixedly disposed on the upper surface of the adapter housing, and the slide rail is fixedly disposed on the lower surface of the adapter housing.

3. The variable spacing adapter device for eight-gang pipettors according to claim 1, wherein a plurality of top adapter heads are provided, and the number of top adapter heads is equal to the number of bottom adapter heads.

4. The variable spacing adapter device for an eight-gang pipette as claimed in claim 1, wherein the number of hoses is plural, and each corresponding top adapter and bottom adapter is connected by one hose.

5. The variable spacing adapter device for an eight gang pipette as defined in claim 1, wherein the connection between the flexible tube and the top adapter and the bottom adapter is an airtight connection.

6. The variable spacing adapter device for an eight-gang pipette as claimed in claim 1, wherein the bottom adapter head is slidably disposed on the slide rail through a slot.

7. The variable spacing adapter device for an eight-gang pipette as defined in claim 1, wherein the top adapter head comprises a first adapter part and a second adapter part, the first adapter part and the second adapter part are communicated with each other, and the first adapter part is used for being matched and connected with a conventional 0.5-10uL eight-gang pipette tip; the second adapting part is used for being connected with the hose in a sealing mode.

8. The device according to claim 1, wherein the slide rail comprises a first rail and a second rail, and the first rail and the second rail are arranged in parallel.

9. The eight gang pipette adapted variable spacing adapter device of claim 1, wherein the bottom adapter head comprises an upper adapter part and a lower adapter part, the interiors of the upper adapter part and the lower adapter part are communicated, and the upper adapter part is used for being in sealing connection with the hose; the lower adapting part is used for being in adaptive connection with the pipette tip.

10. The variable-spacing adapting device applicable to the eight-gang pipette as claimed in claim 6, wherein the clamping groove comprises a first clamping block and a second clamping block, an arc-shaped clamping portion is arranged at the joint of the first clamping block and the second clamping block, and the first clamping block and the second clamping block clamp the upper adapting portion of the bottom adapting head in the arc-shaped clamping portion to realize the connection between the bottom adapting head and the clamping groove.