CN115281211B - Insecticidal composition and application thereof in preventing and controlling eggplant thistle - Google Patents
Insecticidal composition and application thereof in preventing and controlling eggplant thistle Download PDFInfo
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- CN115281211B CN115281211B CN202210754194.9A CN202210754194A CN115281211B CN 115281211 B CN115281211 B CN 115281211B CN 202210754194 A CN202210754194 A CN 202210754194A CN 115281211 B CN115281211 B CN 115281211B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
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Abstract
The invention relates to the technical field of pesticides, discloses an insecticidal composition and application thereof in preventing and controlling eggplant thistle, and in particular relates to application of an insecticidal composition of thiamethoxam and tolfenpyrad in preventing and controlling eggplant thrips. The insecticidal composition consists of thiamethoxam, tolfenpyrad and an auxiliary agent, wherein the weight ratio of the thiamethoxam to the tolfenpyrad is 3:1-1:1. The insecticidal composition can improve the use efficiency of pesticides, improve the effect of preventing and controlling eggplant thrips, has obvious synergistic effect, reduces the use amount of pesticides, reduces the medication cost of farmers, and is beneficial to the safety and environmental protection of environment and food.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to application of an insecticidal composition in preventing and controlling eggplant thistle.
Background
Thiamethoxam, a second generation nicotine high-efficiency low-toxicity pesticide, the chemical formula is C 8 H 10 ClN 5 O 3 S, has stomach toxicity, contact killing and systemic activity to pests, and is used for foliar spraying and soil root irrigation treatment. The pesticide is quickly absorbed by the plant after being applied and is transmitted to various parts of the plant, and has good prevention effect on piercing-sucking pests such as aphids, plant hoppers, leafhoppers, whiteflies and the like.
Tolfenpyrad, english name: tolfennpyrad of formula C 21 H 22 ClN 3 O 2 The pure product is white solid powder. The novel pyrazole heterocyclic insecticidal acaricide developed by Mitsubishi chemical company in Japan has the main action mechanism of preventing oxidative phosphorylation of insects, and also has the functions of killing eggs, inhibiting food intake, inhibiting oviposition and sterilizing.
Eggplant thrips are vegetable pests, about 6000 are known, and 336 are recorded in China. The thrips are small and hidden on the back of the leaves, and suck the juice on the tender stems and leaves, so that the leaves shrink. After the eggplant flowers, the eggplant is damaged by thrips, so that the pollination is poor, the fruits are malformed, and the eggplant falls off when the fruits are serious. The thrips are fast in breeding speed, adults can spawn in stems, leaves and flowers, and nymphs are good in concealment. The thrips are used for sucking the juice of heart leaves, tender stems and young fruits by adult and nymphs, so that the buds and leaves of the damaged plants are curled, the heart leaves can not be unfolded, and a cluster phenomenon occurs; the young fruits are deformed and drop fruits when serious. The damaged pericarp is rough and has marks and little fuzz, or has brown waves, or the pericarp is covered with 'scale' and is deformed. Therefore, when spraying the pesticide, if the spraying position is not right, the spraying time is not right, or the pesticide is misused, the factors can reduce the control effect, the pesticide resistance of pests is increased, the pests are exploded greatly, and the yield of eggplants is reduced or the quality of eggplants is poor.
Disclosure of Invention
The invention aims to provide an insecticidal composition and application thereof in preventing and controlling eggplant thistle.
The technical scheme of the invention is as follows:
the composition consists of thiamethoxam, tolfenpyrad and an auxiliary agent, and is prepared into water dispersible granules, wherein the weight ratio of the thiamethoxam to the tolfenpyrad is 3:1-1:1, and the preferable weight ratio is 3:1.
Further, the content of the effective active ingredients of thiamethoxam and tolfenpyrad is 20% -80%, the preferable content of the effective active ingredients is 40% -60%, and the optimal content of the effective active ingredients is 60%.
Furthermore, the insecticidal composition is applied to preventing and controlling eggplant thrips.
Further, the auxiliary agent is one or more selected from dispersing agents, wetting agents, binders, defoamers, PH regulators and fillers.
The dispersing agent is selected from one or more of alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkylphenol polyoxyethylene ether formaldehyde condensate phosphate, methylene dinaphthyl sodium sulfonate, lignin sodium salt or calcium salt, melamine formaldehyde resin, alkyl naphthalene sulfonate, EO-PO block polyether, styrene phenol polyoxyethylene ether phosphoric acid, alkyl naphthalene formaldehyde condensate sulfonate, alkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether sulfonate, phenethyl phenol polyoxyethylene ether phosphate, alkyl polyoxyethylene ether sulfonate, polyoxyethylene polyoxypropylene ether block copolymer, fenvalerate, dodecyl polyoxyethylene ether phosphate, fatty alcohol polyoxyethylene ether phosphate, nonylphenol polyoxyethylene ether phosphate, naphthalene sulfonic formaldehyde condensate sodium salt block copolymer, comb-type polycarboxylate and sodium polycarboxylate; the wetting agent is one or more selected from sodium dodecyl benzene sulfonate, sodium diisobutyl naphthalene sulfonate, sorbitan fatty acid ester, sulfodocyl sodium salt, alkyl succinic acid sulfonate, phenethyl phenol polyoxyethylene ether phosphate, alkyl sulfate, alkyl sulfonate, alkyl naphthalene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, triphenyl ethylphenol polyoxypropylene polyoxyethylene block polymer, sodium dodecyl sulfate, styrene phenol formaldehyde resin propylene oxide block polyether, castor oil polyoxyethylene ether, fatty alcohol polyoxyethylene ether, alkyl glycoside and fatty alcohol polyoxyethylene ether sodium sulfonate; the binder is one or more selected from water, starch, polyvinylpyrrolidone, polyethylene glycol, glucose and polyvinyl alcohol; the PH regulator is one or more selected from glacial acetic acid, citric acid, light calcium, potassium dihydrogen phosphate and triethanolamine; the defoamer is one or more selected from organic silicone, fatty alcohol and fatty acid; the filler is selected from the group consisting of: white carbon black, calcined kaolin, ammonium sulfate, potassium sulfate, talcum powder, diatomite, starch and urea.
Further, the preparation method of the insecticidal composition comprises the steps of re-preparing wettable powder or water dispersible granules into water dispersible granules by the water dispersible granules, mixing the composition raw medicines with an auxiliary agent according to a certain proportion to form a mixture, and granulating by a fluidized bed granulation method, a spray granulation method or a disc granulation method to obtain the insecticidal composition. In the mixing process, the raw materials are crushed by air flow to ensure that the fineness of the powder particles meets the requirement, and the products are uniformly mixed by using a double-screw mixer and a coulter mixer for multiple times. The preparation has good fluidity, convenient use, no dust flying, safety and reliability.
Further, the dosage of the active ingredients of the insecticidal composition is 54-90 g/hectare.
The insecticidal composition is prepared into water dispersible granules and comprises the following components in percentage by weight: 10-60% of thiamethoxam, 5-40% of tolfenpyrad, 1-20% of dispersing agent, 1-10% of wetting agent, 1-20% of binder, 0-3% of defoaming agent, 0-4% of PH regulator and 100% of filler.
Compared with the prior art, the composition provided by the invention has the following beneficial effects: (1) Compared with a single dose, the composition has obvious synergy on eggplant thrips, and the control effect is obviously improved; (2) The pesticide is low in toxicity and high in efficiency, the pesticide dosage is reduced, the residual quantity of the pesticide on crops is reduced, and the safety interval period is short; (3) has quick and long-acting effects; (4) delay pest resistance; (5) has good control effect on thrips and nymphs.
Detailed Description
The invention is further illustrated by the following examples, which are given by way of percentage, but are not limited thereto.
Application example one
Example 1 60% thiamethoxam tolfenpyrad water dispersible granule
45.92% of 98% thiamethoxam crude drug, 15.31% of 98% of tolfenpyrad crude drug, 4% of sodium lignin sulfonate, 4% of EO-PO block polyether, 6% of sorbitan fatty acid ester, 0.2% of silicone defoamer, 1% of polyethylene glycol and 100% of calcined kaolin are added to prepare 60% thiamethoxam tolfenpyrad water dispersible granule.
Example 2 60% thiamethoxam tolfenpyrad water dispersible granule
45.92% of thiamethoxam original drug, 15.31% of tolfenpyrad original drug, 3% of alkylphenol polyoxyethylene ether formaldehyde condensate phosphate, 3% of alkyl polyoxyethylene ether sulfonate, 2% of alkyl succinic acid sulfonate, 5% of alkyl glycoside, 0.3% of fatty alcohol, 6% of starch and 100% of ammonium sulfate are added to prepare the 60% thiamethoxam tolfenpyrad water dispersible granule.
Example 3 60% thiamethoxam tolfenpyrad water dispersible granule
45.92% of 98% thiamethoxam crude drug, 15.31% of 98% of tolfenpyrad crude drug, 7% of alkylphenol ethoxylate formaldehyde condensate sulfate, 2% of sodium dodecyl benzene sulfonate, 3% of diisobutyl naphthalene sulfonate, 0.5% of fatty acid, 0.8% of polyvinylpyrrolidone, 3% of glucose, 2% of light calcium, 0.3% of citric acid and 100% of starch are added to prepare 60% thiamethoxam tolfenpyrad water dispersible granule.
Example 4 60% thiamethoxam tolfenpyrad water dispersible granule
45.92% of 98% thiamethoxam crude drug, 15.31% of 98% of tolfenpyrad crude drug, 3% of melamine formaldehyde resin, 5% of sodium polycarboxylate, 2% of phenethyl phenol polyoxyethylene ether phosphate, 4% of sodium dodecyl sulfate, 0.4% of fatty alcohol, 1% of polyvinyl alcohol, 0.5% of glacial acetic acid, 5% of white carbon black and 100% of starch are added to prepare the 60% thiamethoxam tolfenpyrad water dispersible granule.
Example 5 80% thiamethoxam tolfenpyrad water dispersible granule
61.22% of thiamethoxam raw material, 20.40% of tolfenpyrad raw material, 2% of dodecyl polyoxyethylene ether phosphate, 3% of sodium polycarboxylate, 3% of alkyl naphthalene sulfonate, 5% of castor oil polyoxyethylene ether, 0.2% of silicone defoamer and 100% of starch to prepare 80% thiamethoxam tolfenpyrad water dispersible granule.
Example 6 40% thiamethoxam tolfenpyrad water dispersible granule
The thiamethoxam and tolfenpyrad water dispersible granule is prepared from the raw material of 30.61% of thiamethoxam, 10.20% of tolfenpyrad, 4% of alkylphenol ethoxylate phosphate, 3% of fatty alcohol polyoxyethylene ether phosphate, 6% of sulfodocusate sodium salt, 3% of glucose, 0.1% of fatty acid, 1% of potassium dihydrogen phosphate, 2% of citric acid and 100% of talcum powder.
Example 7 30% thiamethoxam tolfenpyrad water dispersible granule
The thiamethoxam and tolfenpyrad water dispersible granule is prepared from 23.00% of thiamethoxam crude drug, 7.65% of tolfenpyrad crude drug, 3% of methylene dinaphthyl sulfonate, 2% of polyoxyethylene polyoxypropylene ether block copolymer, 8% of alkylphenol formaldehyde resin polyoxyethylene ether, 1% of silicone defoamer, 2% of polyvinylpyrrolidone, 1% of citric acid, 4% of ammonium sulfate and 100% of white carbon black.
Example 8 20% thiamethoxam tolfenpyrad Water dispersible granule
10.20 percent of thiamethoxam original drug, 10.20 percent of 98 percent of tolfenpyrad original drug, 5 percent of naphthalene sulfonic acid formaldehyde condensate sodium salt block copolymer, 4 percent of styrylphenol formaldehyde resin propylene oxide block polyether, 4 percent of glucose, 2 percent of water, 8 percent of diatomite, 20 percent of starch and 100 percent of kaolin are added to prepare the 20 percent thiamethoxam tolfenpyrad water dispersible granule.
Implementation example II:
tolfenpyrad, thiamethoxam and mixed pair eggplant thrips indoor combined toxicity determination experiment 1 purpose
The suitability of thiamethoxam and tolfenpyrad to eggplant thrips is determined and evaluated by determining and evaluating the toxicity of the thiamethoxam and tolfenpyrad to eggplant thrips in different mixing combinations, so that scientific basis is provided for research and development of thiamethoxam and tolfenpyrad mixtures.
2. Test design
2.1 Test agent
98% of thiamethoxam (deltamethrin); tolfenpyrad (indoxacarb) 98% of the original drug.
2.2 pharmaceutical formulation
The test agent thiamethoxam crude drug and tolfenpyrad crude drug are respectively prepared into 1.0X104 mg/L mother liquor by taking acetone as a solvent, and the mother liquor is placed in a refrigerator for standby. On the basis of the pre-test, acetone (film method and aqueous solution containing 0.1% tween 80 (leaf-dipping method diluted mother liquor, 5 concentrations per treatment were designed, respectively.
The ratio of the effective components of the two compounds is 7:1, 5:1, 3:1, 1:1 and 1:3 on the basis of the pre-test, and the effective components are respectively prepared into 1.0X10 4 mg/L mother liquor is placed in a refrigerator for standby. And diluting the mother solution with acetone (medicinal film method) and aqueous solution containing 0.1% Tween 80 (leaf soaking method), wherein each treatment has 5 concentrations, and 80 healthy and consistent palm thrips adults are treated at each concentration and divided into 4 repetitions, and the aqueous solution containing 0.1% Tween 80 is used as a control.
Thiamethoxam (a): 160. 80, 40, 20, 10mg/L
Tolfenpyrad (B): 80. 40, 20, 10, 5.0mg/L
A:B(7:1)：140、70、35、17.5、8.75mg/L
A:B(5:1)：116、58、29、14.5、7.25mg/L
A:B(3:1)：104、52、26、13、6.5mg/L
A:B(1:1)：96、48、24、12、6.0mg/L
A:B(1:3)：92、46、23、11.5、5.75mg/L
2.3 experiment repetition
The test agent was treated 4 times per concentration, 2 centrifuge tubes per repetition, 10 healthy and consistent thrips palmi adults in each centrifuge tube, i.e. 20 healthy and consistent thrips palmi adults per repetition.
3 test method
With reference to the biological standard methods NY/T1154.14-2008 and NY/T1154.7-2006, the leaf Guan Yaomo method was modified to 1 treatment with a leaf dipping time of 10s.
Preparing a medicine film centrifuge tube: adding 5mL of acetone diluted liquid medicine into a centrifuge tube, continuously rolling to enable the liquid medicine to be uniformly attached to the tube wall to form a uniform medicine film, pouring out the redundant liquid medicine, heating the needle tip of an insect needle by using an alcohol lamp after the medicine film is naturally dried, and then pricking a plurality of small holes on the tube cover and the bottom of the centrifuge tube, wherein the hole diameter is used for preventing thrips adults from escaping.
Leaf soaking: preparing fresh nontoxic purple cabbage leaves into leaf discs with the diameter of 2.0cm by using a puncher, immersing the leaf discs in the same concentration of medicine liquid diluted by using a Tween 80 aqueous solution containing 0.1% for 10s, naturally airing the leaf discs, and transferring 5 leaf discs with medicine into a medicine film centrifuge tube containing the same medicine liquid concentration. The adult thrips palmi is transferred into a centrifuge tube containing a drug membrane with a drug leaf dish by using a fluke device. In the blank control treatment, a drug film centrifuge tube is prepared by adopting the same amount of acetone without a medicament, the leaf is immersed by adopting the same amount of acetone without the medicament and 0.1% Tween 80 aqueous solution, and the rest treatment methods are consistent with the above-described methods. Each treatment was repeated 4 times, every 2 centrifuge tubes, 10 healthy identical thrips palmi adults in each centrifuge tube, i.e., 20 healthy identical thrips palmi adults in each centrifuge tube.
Finally, the centrifuge tubes are placed in an artificial constant temperature insect culturing room with temperature (25+/-1 ℃), illumination (light-dark ratio of 16:8) and humidity (60+/-5%) for culturing. After 48 hours, observation is carried out, the number of dead insects and the number of living insects are recorded, and the death rate and the corrected death rate are calculated.
4 data investigation and statistical analysis
4.1 investigation time and method
After 48h of treatment, the number of dead and alive insects per treatment was checked. Death criteria: the pen point of the zero-number writing brush is used for touching the insect body of the test insect, obvious poisoning symptoms are caused, and the death is caused by the failure of a normal crawler. Record raw data for each process all repetitions.
4.2 method for evaluating synergy
The mortality rate of the blank control is less than 5% without correction; the death rate of the blank control is 5% -20%, and the corrected death rate of each treatment is calculated by using an Abbott formula; the mortality rate of the blank control is more than 20%, and the test needs to be reworked.
The results of the test were calculated using DPS data processing software to calculate the virulence regression equations for the two single doses and five ratios of the test agents and the LC50, LC90 and 95% confidence limits, respectively, and all duplicate raw data for each treatment were recorded.
And calculating a co-toxicity coefficient (CTC) according to a grand cloud Peel method, and evaluating the synergistic effect of the mixed medicament according to the co-toxicity coefficient (CTC), wherein CTC is equal to or less than 80 and is antagonistic, CTC is equal to or less than 120 and is additive, and CTC is equal to or more than 120 and is synergistic. The co-toxicity coefficient (CTC) is calculated according to formulas (3), (4) and (5).
The co-toxicity coefficient (CTC) of each compounding combination was determined by the Sun & Johnson (1960) co-toxicity coefficient method (CTC) with reference to the biological standard method NY/T1154.7-2006. Namely CTC is less than or equal to 80 and is antagonistic, CTC 80 is additive, CTC 120 is synergistic, CTC is more than or equal to 120. The calculation formula of the co-toxicity coefficient is as follows
Formula (1): p= (K/N) ×100
Wherein: p is mortality (%);
k is the number of dead insects (head);
n is the total number of insects (head) processed.
Formula (2): p (P) 1 ＝[(P t -P 0 )/(1-P 0 )]×100
Wherein: p1 is corrected mortality (%);
pt is the mortality (%) of treatment;
p0 is blank mortality (%).
Equation (3): ti= (S/T) ×100
Wherein: TI is the toxicity index measured by the test agent;
s is LC50 (mg/L) of the standard medicament;
t is LC50 (mg/L) of the test agent;
equation (4): tti=ti A ×P A +TI B ×P B
Wherein: TTI is the theoretical toxicity index of the mixture;
TIA is the virulence index of agent a;
PA is the percentage (%) of the a agent in the mix;
TIB is the virulence index of agent B;
PB is the percentage (%) of the B medicament in the mixture.
Equation (5): ctc= (ATI/TTI) ×100
Wherein: CTC is co-toxicity coefficient;
ATI is the actual measured toxicity index of the mixture;
TTI is the theoretical toxicity index of the mixture.
5 experimental results
TABLE 1 results of indoor Combined toxicity measurements of Difenoxanil and thiamethoxam in different ratios on adult thrips eggplant
As shown in the test results Table 1, the test agent thiamethoxam has good toxicity to the adult thrips palmi, the LC50 value is 39.5509mg/L, and the LC90 value is 194.5732mg/L; tolfenpyrad has higher toxicity, the LC50 value is 23.5162mg/L, and the LC90 value is 99.9215mg/L; the toxicity (LC 50) of the two medicaments to the adult thrips palmi is 40.0390, 31.0630, 26.9306, 24.2785 and 23.2567mg/L respectively, and the co-toxicity coefficient CTC is 91.02, 114.33, 125.47, 121.49 and 112.52 respectively. The thiamethoxam and tolfenpyrad have synergy of different degrees in the range of 3:1-1:1 of experimental design proportion, wherein the co-toxicity coefficient (CTC) of the thiamethoxam and tolfenpyrad is the largest, and the synergy is remarkable.
Application example three: example 1 field efficacy test for controlling eggplant thrips
1 purpose of test
The 60% thiamethoxam tolfenpyrad water dispersible granule developed by Shaanxi Mebang pharmaceutical industry group Co-Ltd is verified to have an effect of preventing and controlling eggplant thrips and safety to eggplants, the field use dosage and application technology are defined, and scientific basis is provided for pesticide registration.
2 basis of test
Pesticide registration field efficacy test quality management Specification (NY/T2885-2016) of agricultural industry Standard of the people's republic of China; pesticide field efficacy test criterion (I) (GB/T17980.13-2000).
3 experiment site
The test is arranged in a modern agricultural demonstration park of Juxin in Taigu district in Jinzhong, shanxi province.
4 selection of test subjects, crops and varieties
Test object: thrips palmi Thrips palmi Karny; test crop: eggplant (black eggplant king) Solanum melongena L, has good growth vigor.
5 test design and arrangement
5.1 dosage and numbering of the medicament
TABLE 2 test design of reagents for test
5.2 times and times of application
The initial application of the thrips is started, the medicament is weighed according to the dosage required by each treatment, and the conventional spraying of water is carried out, so that the spraying is required to be uniform and consistent, and the repeated spraying and the leakage spraying are avoided. The blank control was sprayed with water. At 20 days 6 and 2021, the drug was applied 1 time in the initial occurrence of thrips.
5.3 usage Capacity
The water consumption is 45L/mu.
5.4 investigation time and times
The cardinality survey is performed according to the protocol requirements:
first investigation: the number of insect population was investigated and recorded before administration on day 20, 6, 2021.
Second investigation: the number of live insects was recorded on day 21, 6, 2021.
Third investigation: the number of live insects was recorded at 2021, 6 and 23.
Fourth investigation: the number of live insects was recorded on day 27, 6, 2021.
Fifth investigation: the number of live insects was recorded at 2021, 6 and 30.
5.5 method for calculating efficacy
Wherein: PT 0-number of pre-drug insects in the drug treatment area;
PT 1-number of insects after drug treatment in the drug treatment area;
CK 0-predrug number in the control area;
CK 1-number of insects after drug administration in the blank control area;
the significance of the differences between the treatments was analyzed using the SPSS17.0 software DMRT method.
6 experimental results
Table 3 example 1 results of field efficacy test for controlling eggplant thrips
The test results are analyzed by adopting a DMRT method, and the analysis shows that: after 1d of the medicine, the difference between the control effect of treatment C and the control effect of treatment A is extremely remarkable, the difference between the control effect of treatment C and the control effect of treatment B is not remarkable, and the difference between the control effect of treatment A and the control effect of treatment B is not remarkable; the difference between the control effects of treatment C, treatment D and treatment E reaches a significant level, and the difference between the control effects of treatment A, B and treatment D, E does not reach a significant level. 3d after the medicine, the difference between the control effect of treatment C and the control effect of treatment A reaches an extremely remarkable level, and the difference between the control effect of treatment A and the control effect of treatment B reaches a remarkable level; the difference between the control effects of treatment C and treatment D is up to a significant level, the difference between the control effects of treatment A and treatment D, E is not up to a significant level, and the difference between the control effects of treatment B and treatment D, E is not up to a significant level. The prevention effect difference between treatments A, B, C reaches a very significant level after 7d of the medicine; the control effect difference between treatment C and D, E reaches a very significant level, the control effect difference between treatment B and D, E does not reach a significant level, and the control effect difference between treatment A and D, E reaches a very significant level. After the medicine is taken for 10 days, the control effect difference between the treatment C and the treatment A reaches an extremely remarkable level, the control effect difference between the treatment C and the treatment B reaches a remarkable level, and the treatment A and the treatment B also reach a remarkable level; the difference of the control effects of treatment C and D, E reaches a significant level, the difference of the control effects of treatment B and D, E does not reach a significant level, and the control effects of treatment A, D and E reach a significant level.
Application example four: example 2 field efficacy test for controlling eggplant thrips
Experimental purposes and experiments are according to the same application example III.
1 experiment site
The test was arranged in Haikou Longquanzhen, hainan province.
2 selection of test subjects, crops and varieties
Test object: thrips (thread palmi); test crop: eggplant (variety: purple long eggplant).
3 dosage and treatment number of the medicine
4 times and times of application
1 dose is applied in total on the 11 th and 6 th 2019 days.
5 usage capacity
Test water consumption: 30m2 per cell, the liquid medicine amount per cell is 2L of water and 667L/hectare.
6 investigation time, times and method
Basal investigation was performed before the drug (day 11, month 6); 5 times of investigation were performed 1d (day 7 of 11 months), 3d (day 9 of 11 months), 7d (day 13 of 11 months) and 10d (day 16 of 11 months) after the administration.
According to SOP-IT016-v2.0 pesticide field efficacy test for controlling vegetable thrips, 10 eggplants are selected in each district, 3 leaves are fixedly selected at the middle upper part of each eggplant, and 30 leaves are taken in total.
7 medicine effect calculating method
Calculating the rate of reduction of insect population and the prevention and treatment effect according to the formula (1) and the formula (2):
wherein: CK is the rate of reduction of the insect population in the blank control area; PT is the rate of reduction of the insect population in the treatment area with the agent.
8 experimental results
Table 4 example 2 results of field efficacy test for controlling eggplant thrips
Note that lowercase letters indicate differences between treatments at the 0.05 level and uppercase letters indicate differences between treatments at the 0.01 level.
As can be seen from table 4, on day 1 after the drug, the control effects of the three treatments of 60% thiamethoxam tolfenpyrad WG 54 g/ha, 72 g/ha, 90 g/ha were 64.84%, 78.72% and 82.22%, respectively, and the control agents of 25% thiamethoxam WG 75 g/ha and 30% tolfenpyrad SC76.5 g/ha were 64.70% and 62.28%, respectively. Analysis of variance shows that: the control effect of 60% thiamethoxam tolfenpyrad WG treatment 2 and treatment 3 is significantly higher than that of treatment 1 and two control agents; there was no significant difference between the control efficacy of treatment 1 and the two control agents.
On day 3 after the drug, the control effects of three treatments of 60% thiamethoxam tolfenpyrad WG 54 g/hectare, 72 g/hectare and 90 g/hectare are 76.50%, 82.58% and 88.47%, and the control agents of 25% thiamethoxam WG 75 g/hectare and 30% tolfenpyrad SC76.5 g/hectare are 76.78% and 73.68%, respectively. Analysis of variance shows that: the control effect of 60% thiamethoxam tolfenpyrad WG treatment 3 is significantly higher than that of treatment 1, treatment 2 and two control agents; treatment 2 had significantly higher control efficacy than the two control agents.
On day 7 after the drug, the control effects of three treatments of 60% thiamethoxam tolfenpyrad WG 54 g/hectare, 72 g/hectare and 90 g/hectare are 81.67%, 84.88% and 89.71%, and the control agents of 25% thiamethoxam WG 75 g/hectare and 30% tolfenpyrad SC76.5 g/hectare are 83.20% and 82.27%, respectively. Analysis of variance shows that: the control effect of 60% thiamethoxam tolfenpyrad WG treatment 3 is significantly higher than that of treatment 1, treatment 2 and two control agents; there was no significant difference between the control efficacy of treatment 1, treatment 2 and the two control agents.
On the 10 th day after the medicine, on the 3 rd day after the medicine, the control effects of three treatments of 60% thiamethoxam tolfenpyrad WG 54 g/hectare, 72 g/hectare and 90 g/hectare are 79.91%, 83.43% and 86.79%, respectively, and the control effects of treatments of the control medicines of 25% thiamethoxam WG 75 g/hectare and 30% tolfenpyrad SC76.5 g/hectare are 81.70% and 80.91%, respectively. Analysis of variance shows that: the control effect of 60% thiamethoxam tolfenpyrad WG treatment 3 is significantly higher than that of treatment 1, treatment 2 and two control agents; there was no significant difference between treatment 1 and the two control agents.
In a word, experiments show that 60% thiamethoxam tolfenpyrad WG has good control effect on eggplant thrips and has a longer lasting period. The method is safe to crops in the dosage range of test use, and can be popularized and applied in production. The recommended application amount is 54-90 g/hectare.
Application example five: example 3 field efficacy test for controlling eggplant thrips
The experimental purpose, experimental basis, medicament design and dosage are the same as the application example IV.
1 experiment site
The test was arranged in the Wuqing district of Tianjin city.
2 selection of test subjects, crops and varieties
Test object: thrips palmi (Thri ps palmi Karny); test crop: the eggplant is of the type "Tianjin fast garden eggplant".
3 times and times of application
The drug was administered 1 time throughout the trial period on day 22 of 10 months 2019.
4 usage capacity
50 liters of pesticide liquid is sprayed per mu, and the spraying liquid amount per hectare is 750 liters.
5 investigation time and times
The number of live larvae of asparagus caterpillar on cabbage eggplant plants was investigated and marked on day 1, day 3, day 7 and day 10 after the drug. A total of 5 surveys.
6 drug effect calculation method
And performing variance analysis on the control effect data of each cell by using a DMRT method, and comparing the significance of the difference between the average control effects of the medicament treatment by using an SSR method. The control effect (%) was calculated according to the following respective methods.
CK0: pre-drug ostium base for blank control zone, CK1: number of live insects after the drug in the blank control area;
PT0: pre-drug ostium base, PT1: number of living insects after the treatment of the medicine.
7 experimental results
Table 5 example 3 results of field efficacy test for controlling eggplant thrips
As can be seen from Table 5, the spraying treatment of example 3 with a dosage of 120-150 ml/ha or an active ingredient content of 72-90 g has better quick-acting insecticidal activity on thrips on eggplant, and the control effect on 1 st and 3 rd days after the treatment is respectively above 75% and 82%, which is superior to or equivalent to the corresponding dosage treatment effect of the control medicament; the lasting period can be maintained for more than 10 days; the prevention effect on the 7 th and 10 th days after the medicine reaches more than 88% and 85% respectively, which is superior or equivalent to the prevention effect of the corresponding dose treatment of the two control medicines. All the medicaments are treated correspondingly, so that the eggplant plant pesticide is safe to eggplant crops and has no phytotoxicity phenomenon.
Application example six: the experimental purpose, experimental basis, medicament design and dosage and the method for calculating the efficacy of the field test for preventing and treating the thrips of the eggplant are the same as those of the application example III.
1 experiment site
The test was set up in the Wu point city of Hubei province.
2 selection of test subjects, crops and varieties
Test object: thrips palmi; test crop: eggplant is of the type Solanum torvum.
3 times and times of application
The medicine is applied 1 time in 2021, 8 and 16 days.
4 usage capacity
50 liters of pesticide liquid is sprayed per mu, and the spraying liquid amount per hectare is 750 liters.
5 investigation time and times
The number of the investigation before the medicine is counted, and the control effect investigation is carried out 1 day, 3 days, 7 days and 10 days after the medicine. A total of 5 surveys.
6 drug effect calculation method
7 experimental results
Table 6 example 4 results of field efficacy test for controlling eggplant thrips
From table 6, it can be seen that the 60% thiamethoxam tolfenpyrad water dispersible granule of example 4 has excellent effect of controlling eggplant thrips and is safe for crops. In the recommended production practice, 60% thiamethoxam tolfenpyrad water dispersible granule is used for spraying treatment, the recommended dosage of the preparation is 6g/667 square meter-10 g/667 square meter, namely the dosage of the active ingredient is 54g/hm2-90 g/hm2, the dosage of the liquid medicine is 50L/667 square meter, and the preparation is applied for 1 time in the initial stage of thrips occurrence.
Claims (6)
1. An application of an insecticidal composition in preventing and controlling eggplant thistle, which is characterized in that: the insecticidal composition consists of thiamethoxam and tolfenpyrad, and an auxiliary agent, and is prepared into 60% thiamethoxam and tolfenpyrad water dispersible granules, wherein the weight ratio of the thiamethoxam to tolfenpyrad is 3:1;
the auxiliary agent is one or more selected from dispersing agents, wetting agents, binders, defoamers, pH regulators and fillers;
the dispersing agent is one or more selected from alkylphenol ethoxylate formaldehyde condensate sulfate, alkylphenol ethoxylate formaldehyde condensate phosphate, lignin sodium salt or calcium salt, melamine formaldehyde resin, EO-PO block polyether, alkyl polyoxyethylene ether sulfonate and sodium polycarboxylate;
the wetting agent is one or more selected from sodium dodecyl benzene sulfonate, sodium diisobutyl naphthalene sulfonate, sorbitan fatty acid ester, alkyl succinic acid sulfonate, sodium dodecyl sulfate of phenethyl phenol polyoxyethylene ether phosphate and alkyl glycoside;
the binder is one or more selected from polyvinylpyrrolidone, polyethylene glycol, glucose and polyvinyl alcohol;
the pH regulator is one or more selected from glacial acetic acid, citric acid and light calcium carbonate;
the defoamer is one or more selected from organic silicone, fatty alcohol and fatty acid;
the filler is selected from the group consisting of: white carbon black, calcined kaolin, ammonium sulfate and starch.
2. The use according to claim 1, characterized in that: the insecticidal composition comprises the following components in parts by weight: 45.92% of 98% thiamethoxam crude drug, 15.31% of 98% of tolfenpyrad crude drug, 4% of sodium lignin sulfonate, 4% of EO-PO block polyether, 6% of sorbitan fatty acid ester, 0.2% of silicone defoamer, 1% of polyethylene glycol and 100% of calcined kaolin are added to prepare 60% thiamethoxam tolfenpyrad water dispersible granule.
3. The use according to claim 1, characterized in that: the insecticidal composition comprises the following components in parts by weight: 45.92% of thiamethoxam original drug, 15.31% of tolfenpyrad original drug, 3% of alkylphenol polyoxyethylene ether formaldehyde condensate phosphate, 3% of alkyl polyoxyethylene ether sulfonate, 2% of alkyl succinic acid sulfonate, 5% of alkyl glycoside, 0.3% of fatty alcohol, 6% of starch and 100% of ammonium sulfate are added to prepare the 60% thiamethoxam tolfenpyrad water dispersible granule.
4. The use according to claim 1, characterized in that: the insecticidal composition comprises the following components in parts by weight: 45.92% of 98% thiamethoxam crude drug, 15.31% of 98% of tolfenpyrad crude drug, 7% of alkylphenol ethoxylate formaldehyde condensate sulfate, 2% of sodium dodecyl benzene sulfonate, 3% of diisobutyl naphthalene sulfonate, 0.5% of fatty acid, 0.8% of polyvinylpyrrolidone, 3% of glucose, 2% of light calcium, 0.3% of citric acid and 100% of starch are added to prepare 60% thiamethoxam tolfenpyrad water dispersible granule.
5. The use according to claim 1, characterized in that: the insecticidal composition comprises the following components in parts by weight: 45.92% of 98% thiamethoxam crude drug, 15.31% of 98% of tolfenpyrad crude drug, 3% of melamine formaldehyde resin, 5% of sodium polycarboxylate, 2% of phenethyl phenol polyoxyethylene ether phosphate, 4% of sodium dodecyl sulfate, 0.4% of fatty alcohol, 1% of polyvinyl alcohol, 0.5% of glacial acetic acid, 5% of white carbon black and 100% of starch are added to prepare the 60% thiamethoxam tolfenpyrad water dispersible granule.
6. Use according to any one of claims 1 to 5, characterized in that: the dosage of the effective components of the 60% thiamethoxam tolfenpyrad water dispersible granule is 54-90 g/hectare.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101658178A (en) * | 2009-07-22 | 2010-03-03 | 深圳诺普信农化股份有限公司 | Synergistic insecticidal composition |
| CN102657176A (en) * | 2012-04-12 | 2012-09-12 | 广西田园生化股份有限公司 | Tolfenpxrad-containing smoke agent |
| CN103621532A (en) * | 2012-08-27 | 2014-03-12 | 南京华洲药业有限公司 | Synergistic insecticidal composition containing thiamethoxam and tolfenpyrad and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101658178A (en) * | 2009-07-22 | 2010-03-03 | 深圳诺普信农化股份有限公司 | Synergistic insecticidal composition |
| CN102657176A (en) * | 2012-04-12 | 2012-09-12 | 广西田园生化股份有限公司 | Tolfenpxrad-containing smoke agent |
| CN103621532A (en) * | 2012-08-27 | 2014-03-12 | 南京华洲药业有限公司 | Synergistic insecticidal composition containing thiamethoxam and tolfenpyrad and application thereof |
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