CN115264134B

CN115264134B - One-way check valve structure and manufacturing process thereof

Info

Publication number: CN115264134B
Application number: CN202211197979.7A
Authority: CN
Inventors: 关国良; 陈巧玲; 李琦; 金诚; 朱子明
Original assignee: Changzhou Xianxu Medical Technology Co ltd
Current assignee: Changzhou Xianxu Medical Technology Co ltd
Priority date: 2022-09-29
Filing date: 2022-09-29
Publication date: 2023-04-07
Anticipated expiration: 2042-09-29
Also published as: CN115264134A

Abstract

The invention belongs to the technical field of one-way valves, and particularly relates to a one-way check valve structure and a manufacturing process thereof. This one-way check valve structure includes: the separation platform comprises a platform part, an inclined plane part and an extension part which are sequentially arranged from left to right, one end of the separation baffle is a fixed end fixed on the lower side of the left inner side wall of the back pressure bin, and the other end of the separation baffle is a movable end covered on the platform part of the separation platform so as to separate the middle flow channel from the main bin of the back pressure bin; when the fluid flows from left to right, the fluid pushes open the movable end of the separation baffle and then flows into the middle sample outlet channel through the main bin and the capillary channel; and when the fluid flows from right to left, the pressure in the back pressure chamber is increased to press the right end part of the separation baffle plate on the platform part of the separation platform, so that the fluid is prevented from entering the middle flow channel from the middle sample outlet channel.

Description

One-way check valve structure and manufacturing process thereof

Technical Field

The invention belongs to the technical field of one-way valves, and particularly relates to a one-way check valve structure and a manufacturing process thereof.

Background

The existing micro-fluidic valves are mainly divided into active flow regulating valves and passive flow regulating valves (also called active valves and passive valves). The active flow regulating valve (active valve) mainly realizes fluid control by accessing a control element outside a chip, such as accessing a pneumatic device, an electromagnetic device, a centrifugal device and the like to realize the flow control of microfluid. The active flow regulating valve can realize complex microfluidic control, but the external control element can greatly increase the volume and the manufacturing cost of the microfluidic chip. And therefore is not suitable for a microfluidic device requiring miniaturization and low cost.

The passive flow regulating valve (passive valve) mainly depends on the self structure or the mechanical deformation of materials to the external pressure to realize flow regulation, does not need external force and has a relatively simple structure, thereby having more advantages in a low-cost and integrated micro-fluidic system. Common passive flow control valves are membrane valves and fluid passage valves: the membrane valve is a switch which is provided with an elastic membrane between the control flow channel and the liquid flow channel and is controlled by the microfluid valve by utilizing the elastic deformation of the membrane and the relative change of the fluid pressure; classical fluid channel valves, such as tesla valves, achieve unidirectional flow control in a certain sense by designing a specific backflow angle in the fluid channel to increase the resistance to fluid backflow. At present, the passive flow regulating valves can not realize one-way valve control in the true sense, most of film valves are actually two-way valves, and the valve control capability of the film valves depends on the elastic force of the films. Fluid channel valves require their elaborate design and tooling without substantial partitioning of the fluid channels and reaction chambers. Particularly in special application scenarios, such as when heating and pressurizing of fluid are required, the passive flow valve is prone to fluid dissipation due to poor valve control capability, and the reaction system is difficult to stabilize.

In summary, the existing microfluidic valve cannot have the low-cost and precise flow control functions of the microfluidic, and it is necessary to develop a microfluidic valve with a novel structure to improve the flow control effect of the passive flow control valve, so as to achieve the low cost of the microfluidic instrument, and especially meet the requirement of microfluidic operation control in the application scenario of heating and pressurizing reaction.

Disclosure of Invention

The invention aims to provide a one-way check valve structure and a manufacturing process thereof.

In order to solve the above technical problems, the present invention provides a one-way check valve structure, comprising: the sample separation device comprises a middle flow passage, a separation platform and a middle sample outlet passage which are arranged from left to right, wherein the separation platform comprises a platform part, an inclined plane part and an extension part which are sequentially arranged from left to right, and the inclined plane of the inclined plane part extends to the upper right; the back pressure bin is positioned above the separating table, and the inclined plane part and the extending part of the separating table extend into the back pressure bin, wherein a main bin communicated with the middle flow channel is formed by the left inner side wall and the top wall of the back pressure bin and the inclined plane part of the separating table, capillary channels are formed between the top wall and the right side wall of the back pressure bin and the top surface and the right side surface of the extending part, and the capillary channels are communicated with the main bin and the middle sample outlet channel; the separation baffle is made of elastic materials, separates the middle flow channel from the main bin, and is fixed along two side edges of the fluid flowing direction, the left end of the separation baffle is positioned outside the projection of the back pressure bin, the right end of the separation baffle is positioned in the projection of the back pressure bin, and the end part of the separation baffle is covered on the platform part; when the fluid flows from left to right, the separating baffle is elastically deformed by the pressure of the fluid, namely, after the right end is pushed open, the fluid flows into the middle sample outlet channel through the main bin and the capillary channel; and when the fluid flows from right to left, the pressure in the back pressure chamber is increased so as to press the right end part of the separation baffle plate on the platform part of the separation platform and prevent the fluid from entering the middle flow channel from the middle sample outlet channel.

Furthermore, a backflow hole is sunken below the extension part of the separation table and is communicated with the middle sample outlet channel; the capillary channel includes: a transverse channel formed by the top wall of the back pressure bin and the top surface of the extension part; the vertical channel is formed by the right side wall of the back pressure bin and the right side surface of the extension part; wherein the vertical channel communicates the transverse channel and the return orifice.

Furthermore, the depth of the back pressure bin is not more than 1.5mm, the slope of the inclined plane part is not more than 45 degrees, the surface is rough, and the width of the capillary channel is not more than 0.5mm.

Further, the separation baffle is a latex film, a rubber film or a PDMS film.

Furthermore, the width of the reflux hole is not less than 0.5mm, and the height of the reflux hole is 0.5-1 mm higher than that of the middle sample outlet channel.

Further, the one-way check valve structure further includes: the fluid inlet, the inlet main channel and the sample processing chamber are sequentially communicated, and the sample processing chamber is communicated with the middle flow channel; and the heating reaction chamber, the outlet main flow channel and the fluid outlet are communicated in sequence, and the heating reaction chamber is communicated with the middle sample outlet channel.

Further, the one-way check valve structure further includes: the fluid inlet, the fluid outlet and the back pressure bin are all arranged on the upper plate; the lower plate is provided with an inlet main channel, a sample processing chamber, a middle flow channel, a separation table, a middle sample outlet channel, a heating reaction chamber and an outlet main flow channel; and two sides of the separation baffle along the fluid flowing direction are fixed between the upper plate and the lower plate.

In another aspect, the present invention further provides a manufacturing process of the above one-way check valve structure, including: processing an upper plate and a lower plate, wherein the upper plate is provided with a fluid inlet, a fluid outlet and a back pressure bin, and the lower plate is provided with an inlet main channel, a sample processing chamber, a middle flow channel, a separation table, a middle sample outlet channel, a heating reaction chamber and an outlet main channel; processing a separation baffle, and placing the separation baffle between the middle flow channel and the back pressure bin; and integrating the assembled upper plate, the assembled lower plate and the separating baffle.

Further, the method for processing the upper plate and the lower plate comprises the following steps: machine tool machining, injection molding or laser machining.

Further, the integration method comprises the following steps: adhesive material bonding, thermal bonding or super-liter bonding; wherein the thermal bonding conditions are as follows: the pressure is 0.065Mpa, the bonding temperature is 120 ℃, and the pressing time is 37min.

The invention has the beneficial effects that:

(1) The one-way check valve structure of the invention does not need to be externally connected with a control device, and the material is simple and easy to obtain, so the one-way check valve structure has the advantages of small volume, low price and simple manufacture, and is suitable for low-cost miniaturized microfluidic instrument equipment;

(2) Aiming at the problem that the existing passive valve cannot realize real one-way valve control, the invention constructs the back pressure cabin through structural design, realizes real one-way valve control, and greatly optimizes the flow control effect.

(3) The invention simplifies the design of the three-layer structure of the traditional passive valve into a two-layer structure, obtains better valve control effect and greatly reduces the processing difficulty and cost.

(4) The invention optimizes the sealing principle of the one-way valve, and the valve control energy comes from the elasticity of the separating baffle, the gas pressure of the back pressure cabin and the capillary channel and the flow blocking effect of the backflow hole to jointly strengthen the backflow resistance of the micro-fluidic valve, so that the variety of available elastic film materials is increased.

(5) By changing the types of the elastic film materials and the depths of the back pressure bin and the backflow hole, the valve control capacity of the flow control valve can be adjusted, and diversified valve control requirements can be met.

Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and drawings.

In order to make the aforementioned and other objects, features and advantages of the present invention comprehensible, preferred embodiments accompanied with figures are described in detail below.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a cross-sectional view of a preferred embodiment of the one-way check valve construction of the present invention;

FIGS. 2 and 3 are partial cross-sectional views of preferred embodiments of the one-way check valve structure of the present invention;

FIG. 4 is a schematic forward flow diagram of a preferred embodiment of the one-way check valve construction of the present invention;

FIG. 5 is a non-return schematic view of a preferred embodiment of the one-way check valve construction of the present invention;

FIG. 6 is an assembly schematic of a preferred embodiment of the one-way check valve construction of the present invention;

fig. 7 is an opening schematic view of the separation damper of the preferred embodiment of the one-way check valve structure of the present invention.

In the figure:

the upper plate 100, the fluid inlet 110, the fluid outlet 120, the back pressure chamber 130, the main chamber 131, the capillary channel 132, the transverse channel 1321, and the vertical channel 1322;

a lower plate 200, an inlet main channel 210, a sample processing chamber 220, an intermediate flow channel 230, a partition 240, a platform 241, a bevel 242, an extension 243, a reflow hole 244, an intermediate sample outlet channel 250, a heating reaction chamber 260, and an outlet main channel 270;

separating baffle 300, fixed area 310.

Detailed Description

To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.

Example 1

As shown in fig. 1, 2, and 3, the present embodiment provides a one-way check valve structure, including: the sample separation device comprises a middle flow channel 230, a separation table 240 and a middle sample outlet channel 250 which are arranged from left to right, wherein the separation table 240 comprises a platform part 241, an inclined plane part 242 and an extension part 243 which are sequentially arranged from left to right, and the inclined plane of the inclined plane part 242 extends towards the upper right; the back pressure chamber 130 is located above the partition stage 240, and the inclined plane part 242 and the extension part 243 of the partition stage 240 extend into the back pressure chamber 130, wherein the main chamber 131 communicated with the middle flow channel 230 is formed by the left inner side wall and the top wall of the back pressure chamber 130 and the inclined plane part 242 of the partition stage 240, the capillary channel 132 is formed between the top wall and the right side wall of the back pressure chamber 130 and the top surface and the right side surface of the extension part 243, and the capillary channel 132 is communicated with the main chamber 131 and the middle sample outlet channel 250; a partition plate 300 made of an elastic material for partitioning the intermediate flow channel 230 and the main chamber 131, wherein the partition plate is fixed at two sides along the fluid flowing direction, the left end is located outside the projection of the back pressure chamber 130, the right end is located inside the projection of the back pressure chamber 130, and the end part is covered on the platform part 241; when the fluid flows from left to right, the fluid pressure elastically deforms the separation baffle 300 (see fig. 7), that is, after the right end is pushed open, the fluid flows into the middle sample outlet channel 250 through the main chamber 131 and the capillary channel 132; and when the fluid flows from right to left, the pressure in the back pressure chamber 130 is increased to press the right end of the separation baffle 300 against the platform 241 of the separation stage 240, thereby preventing the fluid from entering the intermediate flow channel 230 through the intermediate sample outlet channel 250.

In this embodiment, it is preferable that the separation stage 240 is recessed with a backflow hole 244 below the extension portion 243, and is communicated with the middle sample outlet channel 250; the capillary channel 132 includes: a transverse channel 1321 formed by the top wall of the counter-pressure compartment 130 and the top surface of the extension 243; a vertical channel 1322 formed by the right side wall of the counter pressure compartment 130 and the right side face of the extension 243; wherein the vertical passage 1322 communicates the lateral passage 1321 and the return orifice 244.

In this embodiment, the return orifice 244 may provide some resistance to fluid flowing in the opposite direction; for example, when the fluid in the intermediate sample outlet 250 flows into the return hole 244, the fluid may flow back when encountering the hole wall of the return hole 244, thereby forming a turbulent flow to block the fluid flowing through the intermediate sample outlet 250.

In the present embodiment, the micro-fluid has a characteristic that the influence of the capillary phenomenon is greater than the surface tension of the condensed water droplets in the micro-channel, and the micro-fluid liquid will form a water film on the rough surface of the inclined surface portion 242 and flow out from the fine capillary channel 132.

In this embodiment, in order to achieve the above effect, the depth of the back pressure chamber 130 is not greater than 1.5mm, the slope of the slope 242 is not greater than 45 degrees and the surface is rough, and the width of the capillary channel 132 is not greater than 0.5mm.

In this embodiment, the separation barrier 300 may be, but is not limited to, a latex film or a rubber film.

In this embodiment, preferably, when the liquid flows back, as shown in fig. 5, the backflow hole 244 may be configured to block the backflow, and the width of the backflow hole 244 is not less than 0.5mm and the height of the backflow hole 244 is 0.5 to 1mm higher than the middle sample outlet channel 250.

In this embodiment, the height of the return hole 244 is higher than that of the intermediate sampling passage 250, and a liquid level difference is formed, thereby further preventing the liquid in the intermediate sampling passage 250 from returning.

In one application scenario, the one-way check valve structure further includes: the fluid inlet 110, the inlet main channel 210 and the sample processing chamber 220 are communicated in sequence, and the sample processing chamber 220 is communicated with the intermediate flow channel 230; and a heating reaction chamber 260, an outlet main channel 270, and a fluid outlet 120, which are sequentially communicated, and the heating reaction chamber 260 is communicated with the intermediate sample outlet channel 250.

In this embodiment, optionally, as shown in fig. 4, the fluid enters through the fluid inlet 110, and the pre-loaded reagents (including but not limited to a small amount of water, dry chemical reagent powder, dry biological reagent powder, etc.) contained in the sample processing chamber 220 can pre-process the entering sample, and the possible pre-processing effects include but not limited to sample dilution, DNA extraction, sterilization, etc. After sample pretreatment, the separation baffle 300 is pushed open by the fluid control pushing to pass through the back pressure chamber 130 and the capillary channel 132, and then reaches the heating reaction chamber 260 through the intermediate sample outlet channel 250. At this time, the fluid outlet 120 is closed, the sample is heated and reacted in the heating reaction chamber 260, and at this time, the working state of the one-way check valve structure is as shown in fig. 5, and the elasticity of the partition baffle 300, the gas pressure of the counter pressure bin 130 and the capillary channel 132, and the flow blocking effect of the backflow hole 244 jointly enhance the backflow resistance of the one-way check valve structure, so that the requirements of the application scenario can be met.

In this embodiment, as shown in fig. 6, as an alternative assembly manner of the one-way check valve structure, the one-way check valve structure further includes: the upper plate 100, the fluid inlet 110, the fluid outlet 120 and the back pressure chamber 130 are all arranged on the upper plate 100; a lower plate 200, on which the inlet main channel 210, the sample processing chamber 220, the intermediate flow channel 230, the partition stage 240, the intermediate sample outlet channel 250, the heating reaction chamber 260, and the outlet main flow channel 270 are disposed; and both side edges of the separation baffle 300 in the fluid flow direction are fixed between the upper plate 100 and the lower plate 200; wherein the fluid inlet 110 communicates with the inlet main channel 210 and the outlet main channel 270 communicates with the fluid outlet 120 when the upper plate 100 and the lower plate 200 are connected; referring to fig. 7, the width of the partition board 300 is larger than that of the back pressure compartment 130, so that the fixing zones 310 on both sides of the partition board 300 can be clamped between the upper board 100 and the lower board 200.

In this embodiment, the one-way check valve structure may be applied to a microfluidic chip.

Example 2

On the basis of the embodiment, this embodiment 2 provides a manufacturing process of the one-way check valve structure as described in embodiment 1, including: processing an upper plate 100 provided with a fluid inlet 110, a fluid outlet 120 and a counter pressure compartment 130 and a lower plate 200 provided with an inlet main channel 210, a sample processing chamber 220, a middle flow channel 230, a partition stage 240, a middle outlet channel 250, a heating reaction chamber 260, an outlet main channel 270; a separation baffle 300 is processed and is placed between the middle runner 230 and the back pressure bin 130; the assembled upper plate 100, lower plate 200 and partition plate 300 are integrated.

In this embodiment, the method of processing the upper and

lower plates

100 and 200 may optionally include: machine tool machining, injection molding or laser machining. But not limited to, machining with acrylic sheet material on a CNC machine or laser cutter.

In this embodiment, optionally, the integration method includes: adhesive material bonding, thermal bonding or super-liter bonding; wherein the thermal bonding conditions are as follows: the pressure is 0.065Mpa, the bonding temperature is 120 ℃, and the pressing time is 37min.

In summary, the one-way check valve structure of the present invention has the following advantages:

(1) The one-way check valve structure of the invention does not need external control device materials, and is simple and easy to obtain, thus having the advantages of small volume, low price and simple manufacture and being suitable for the micro-fluidic instrument equipment with low cost and miniaturization.

(2) Aiming at the problem that the existing passive valve cannot realize real one-way valve control, the invention constructs the back pressure cabin through structural design, realizes the real one-way valve control, and greatly optimizes the flow control effect.

(4) The invention optimizes the sealing principle of the one-way valve, integrates the advantages of two designs of the passive film valve and the passive channel valve, greatly enhances the valve control effect and expands the available range of film materials. The valve control capacity of the flow control valve can be adjusted by changing the types of elastic film materials, the depth of the pressurizing cavity and the area of the flow distribution table, and the diversified valve control requirements can be met.

(5) The invention provides an application scenario, and solves the problem of fluid control in heating reaction in a low-cost microfluidic instrument.

The components selected for use in the present application (components not illustrated for specific structures) are all common standard components or components known to those skilled in the art, and the structure and principle thereof can be known to those skilled in the art through technical manuals or through routine experimentation.

In the description of the embodiments of the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In light of the foregoing description of the preferred embodiment of the present invention, many modifications and variations will be apparent to those skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.

Claims

1. A one-way check valve structure, comprising:

the device comprises a middle flow channel (230), a separation table (240) and a middle sample outlet channel (250), wherein the middle flow channel, the separation table (240) and the middle sample outlet channel are arranged from left to right, the separation table (240) comprises a platform part (241), an inclined plane part (242) and an extension part (243) which are sequentially arranged from left to right, and the inclined plane of the inclined plane part (242) extends towards the upper right;

the back pressure bin (130) is positioned above the separating table (240), and the inclined plane part (242) and the extending part (243) of the separating table (240) extend into the back pressure bin (130), wherein the left inner side wall and the top wall of the back pressure bin (130) and the inclined plane part (242) of the separating table (240) form a main bin (131) communicated with the middle flow channel (230), capillary channels (132) are formed between the top wall and the right side wall of the back pressure bin (130) and the top surface and the right side surface of the extending part (243), and the capillary channels (132) are communicated with the main bin (131) and the middle sample outlet channel (250);

a separation baffle (300) which is made of elastic material, separates the middle flow passage (230) and the main chamber (131), and is fixed along two side edges of the fluid flowing direction, the left end is positioned outside the projection of the back pressure chamber (130), the right end is positioned in the projection of the back pressure chamber (130), and the end part is covered on the platform part (241); wherein

When the fluid flows from left to right, the separating baffle (300) is elastically deformed by the pressure of the fluid, namely, after the right end is pushed open, the fluid flows into the middle sample outlet channel (250) through the main bin (131) and the capillary channel (132); and

when the fluid flows from right to left, the pressure in the back pressure chamber (130) is increased to press the right end of the partition board (300) against the platform part (241) of the partition table (240) to prevent the fluid from entering the intermediate flow channel (230) from the intermediate sample outlet channel (250).

2. The one-way check valve structure of claim 1,

the separation table (240) is provided with a backflow hole (244) which is sunken below the extension part (243) and is communicated with the middle sample outlet channel (250);

the capillary channel (132) comprises:

a transverse channel (1321) formed by the top wall of the counter-pressure compartment (130) and the top surface of the extension (243);

a vertical channel (1322) formed by the right side wall of the counter-pressure cabin (130) and the right side face of the extension (243); wherein

The vertical channel (1322) communicates the transverse channel (1321) with the return hole (244).

3. The one-way check valve structure of claim 2,

the depth of the back pressure bin (130) is not more than 1.5mm, the slope of the inclined plane part (242) is not more than 45 degrees, the surface is rough, and the width of the capillary channel (132) is not more than 0.5mm.

4. The one-way check valve structure of claim 3,

the separation baffle (300) is a latex film, a rubber film or a PDMS film.

5. The one-way check valve structure of claim 3,

the width of the backflow hole (244) is not less than 0.5mm, and the height of the backflow hole is 0.5-1 mm higher than that of the middle sample outlet channel (250).

6. The one-way check valve structure of claim 3,

the one-way check valve structure further includes:

the fluid inlet (110), the inlet main channel (210) and the sample processing chamber (220) are communicated in sequence, and the sample processing chamber (220) is communicated with the intermediate flow channel (230); and

a heating reaction chamber (260), an outlet main channel (270), and a fluid outlet (120) which are communicated in sequence, wherein the heating reaction chamber (260) is communicated with the middle sample outlet channel (250).

7. The one-way check valve structure of claim 6,

the one-way check valve structure further includes:

the upper plate (100), the fluid inlet (110), the fluid outlet (120) and the back pressure bin (130) are all arranged on the upper plate (100);

the lower plate (200), the inlet main channel (210), the sample processing chamber (220), the middle flow channel (230), the separating table (240), the middle sample outlet channel (250), the heating reaction chamber (260) and the outlet main flow channel (270) are all arranged on the lower plate (200); and

the two sides of the separation baffle (300) along the flowing direction of the fluid are fixed between the upper plate (100) and the lower plate (200).

8. A process for making a one-way check valve structure as claimed in any one of claims 1 to 7, comprising:

processing an upper plate (100) and a lower plate (200), wherein the upper plate is provided with a fluid inlet (110), a fluid outlet (120) and a counter pressure chamber (130), and the lower plate (200) is provided with an inlet main channel (210), a sample processing chamber (220), a middle flow channel (230), a separation table (240), a middle sample outlet channel (250), a heating reaction chamber (260) and an outlet main channel (270);

a processing separation baffle (300) is arranged between the middle runner (230) and the back pressure bin (130);

the assembled upper plate (100), lower plate (200) and separating baffle (300) are integrated.

9. The manufacturing process according to claim 8,

the method for processing the upper plate (100) and the lower plate (200) comprises the following steps: machine tool machining, injection molding or laser machining.

10. The manufacturing process according to claim 8,

the integration method comprises the following steps: adhesive material bonding, thermal bonding or super-elevated bonding; wherein

The thermal bonding conditions were: the pressure is 0.065Mpa, the bonding temperature is 120 ℃, and the pressing time is 37min.