CN115259556A - Proliferation and cultivation method of anaerobic granular sludge for papermaking wastewater - Google Patents
Proliferation and cultivation method of anaerobic granular sludge for papermaking wastewater Download PDFInfo
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F9/00—Multistage treatment of water, waste water or sewage
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/54—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using organic material
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- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/26—Nature of the water, waste water, sewage or sludge to be treated from the processing of plants or parts thereof
- C02F2103/28—Nature of the water, waste water, sewage or sludge to be treated from the processing of plants or parts thereof from the paper or cellulose industry
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- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
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Abstract
The invention discloses a proliferation and bacterium culture method of anaerobic granular sludge for papermaking wastewater, and relates to the technical field of anaerobic granular sludge. When the proliferation culture of the anaerobic granular sludge for the papermaking wastewater is carried out, the papermaking wastewater is flocculated by using a polyacrylamide flocculant to obtain flocculated papermaking wastewater, a nutrient solution is added into the flocculated papermaking wastewater to carry out anaerobic treatment to obtain an anaerobic treatment solution, nitrogen-containing carbon quantum dots are added into the anaerobic treatment solution to obtain active granular sludge, and a liquid culture medium is used for culturing the active granular sludge to obtain the anaerobic granular sludge for the papermaking wastewater. The proliferation and culture method of the anaerobic granular sludge for the papermaking wastewater has excellent decontamination performance.
Description
Technical Field
The invention relates to the technical field of anaerobic granular sludge, in particular to a proliferation and bacterium cultivation method of anaerobic granular sludge for papermaking wastewater.
Background
In China, straw, wood pulp and the like are mostly adopted as paper making raw materials. The pulping and papermaking production generally comprises the working procedures of pulping, pulp washing, bleaching and papermaking. The pulping in the paper industry includes alkaline pulping, chemi-mechanical pulping and mechanical pulping. The waste water has different generation and discharge of pollutants according to different pulping methods, different raw materials, different pulping yields, different paper making varieties and the existence of chemical recovery, but basically contains a large amount of suspended matters, BOD, COD and partial toxic substances and is treated by adopting a mode of combining a physicochemical method and a biological method.
The anaerobic digestion process is a wastewater treatment process with potential feasibility, which is successfully applied to the wastewater treatment in the fields of life, industry, agriculture and the like in recent years. Upflow Anaerobic Sludge Blanket (UASB) is one of the most competitive anaerobic digestion technologies in current wastewater treatment technologies, showing great potential in treating various kinds of wastewater with different properties, and nearly 80% of the world's anaerobic sewage treatment systems are based on the UASB technology. The UASB has the advantages of strong methane production capacity, low residual sludge yield and the like; another significant advantage of UASB is high pollutant removal efficiency, with Chemical Oxygen Demand (COD) removal rates mostly exceeding 90%. UASB is one of the most efficient and widely used anaerobic reactors, and has good settleability and biological activity.
Disclosure of Invention
The invention aims to provide a proliferation and bacterium cultivation method of anaerobic granular sludge for papermaking wastewater, which aims to solve the problems in the prior art.
A proliferation and bacterium culture method of anaerobic granular sludge for papermaking wastewater mainly comprises the following preparation steps: flocculation treatment, anaerobic treatment, granulation and multiplication culture.
As optimization, the proliferation and culture method of the anaerobic granular sludge for the papermaking wastewater mainly comprises the following preparation steps:
(1) Flocculation treatment: adding a polyacrylamide flocculant into the papermaking wastewater until no obvious floccule is generated, removing the floccule, naturally standing for 20 to 24h to obtain flocculated papermaking wastewater;
(2) Anaerobic treatment: adding a nutrient solution with the mass of 0.1 to 0.2 times of that of the flocculated papermaking wastewater into the flocculated papermaking wastewater, sealing the mixture by using a polyethylene film, and standing the mixture for 30 to 36h at the temperature of 30 to 40 ℃ to obtain an anaerobic treatment solution;
(3) Granulating: adding nitrogen-containing carbon quantum dots with the mass 0.03 to 0.05 times of that of the anaerobic treatment liquid into the anaerobic treatment liquid, stirring at 30 to 40 ℃ and 300 to 500r/min for 40 to 50min, filtering, and drying at 10 to 20 ℃ and 1 to 10Pa for 6 to 8h to prepare active granular sludge;
(4) And (3) proliferation culture: placing the activated granular sludge in a liquid culture medium with the mass of 20-30 times that of the activated granular sludge, standing for 30-36h in an anaerobic environment at 30-40 ℃, filtering, and drying for 6-8h at 10-20 ℃ and 1-10Pa to prepare the anaerobic granular sludge for the papermaking wastewater.
Preferably, the papermaking wastewater in the step (1) is one or a mixture of waste liquor generated by cooking wood pulp, wastewater discharged by a beating machine and a refiner and papermaking machine wastewater.
Preferably, the solvent of the nutrient solution in the step (2) is water, and the composition comprises 20 to 30g/L glucose, 7 to 9g/L magnesium sulfate, 6 to 8g/L calcium chloride, 1 to 2g/L cobalt chloride, 1 to 2g/L ferric chloride, 0.03 to 0.05g/L zinc chloride, 0.08 to 0.1g/sodium chloride, 0.03 to 0.05g/L nickel chloride and 0.2 to 0.3mL/L hydrogen chloride.
As an optimization, the preparation method of the nitrogen-containing carbon quantum dot in the step (3) comprises the following steps: preheating a metal organic framework at 100-120 ℃ for 30-40min in a nitrogen atmosphere, raising the temperature to 250-300 ℃ at the speed of 5-6 ℃/min, standing for 60-80min, raising the temperature to 800-900 ℃, and standing for 2-3h to obtain the nitrogen-containing carbon quantum dot.
Preferably, the liquid medium solvent in the step (4) is water, and the components of the liquid medium solvent comprise 20% of polypeptone, 1% of tryptone, 0.5% of yeast extract, 0.5% of sodium chloride, 5% of beef extract, 0.04% of cysteine and 0.5% of hemin.
Preferably, the anaerobic granular sludge for papermaking wastewater prepared by the proliferation and cultivation method of the anaerobic granular sludge for papermaking wastewater is applied to pollution treatment of waste liquid generated by cooking wood pulp, wastewater discharged by a pulping machine and a refiner and papermaking machine wastewater.
Compared with the prior art, the invention has the following beneficial effects:
the invention carries out flocculation treatment, anaerobic treatment, granulation and enrichment culture in turn when the proliferation culture of the anaerobic granular sludge for the papermaking wastewater is carried out.
Firstly, flocculating treatment is carried out to remove suspended particles in the papermaking wastewater, anaerobic treatment is carried out to propagate anaerobic microorganisms in the residual papermaking wastewater, and targeted microorganism culture is carried out on the papermaking wastewater treatment, so that the microorganisms have good targeted decontamination on the papermaking wastewater.
Secondly, the nitrogen-containing carbon quantum dots obtained by carbonizing the zinc nitrate and the 2-methylimidazole after reaction are granulated, have better adsorption loading effect on microorganisms in the water body, and enable the microorganisms to proliferate on the nitrogen-containing carbon quantum dots, so that the anaerobic granular sludge for the papermaking wastewater has good decontamination effect; and (4) proliferation culture, wherein the liquid culture medium contains a large amount of nutrients, so that the proliferation of microorganisms is promoted, and the using effect is improved.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In order to more clearly illustrate the method provided by the invention, the following examples are used for detailed description, and the method for testing each index of the proliferation and cultivation method of anaerobic granular sludge for papermaking wastewater prepared in the following examples is as follows:
decontamination performance: the proliferation and cultivation method of the anaerobic granular sludge for the papermaking wastewater obtained in each embodiment and a comparative product take the same mass, the anaerobic granular sludge and the comparative product are placed in the same kind of papermaking wastewater with the same light absorption rate, the light absorption rate is measured again after the same time, and the habitual light absorption rate reduction rate is calculated.
Example 1
A proliferation and bacterium culture method of anaerobic granular sludge for papermaking wastewater mainly comprises the following preparation steps:
(1) Flocculation treatment: adding a polyacrylamide flocculant into the papermaking wastewater until no obvious floccule is generated, removing the floccule, and naturally standing for 20 hours to obtain flocculated papermaking wastewater;
(2) Anaerobic treatment: adding nutrient solution which is 0.1 time of the mass of the flocculated papermaking wastewater into the flocculated papermaking wastewater, sealing the flocculated papermaking wastewater by using a polyethylene film, and standing the flocculated papermaking wastewater for 36 hours at 30 ℃ to obtain anaerobic treatment solution; the solvent of the nutrient solution is water and comprises 20g/L glucose, 7g/L magnesium sulfate, 6g/L calcium chloride, 1g/L cobalt chloride, 1g/L ferric chloride, 0.03g/L zinc chloride, 0.08 g/L sodium chloride, 0.03g/L nickel chloride and 0.2mL/L hydrogen chloride;
(3) Granulating: zinc nitrate, 2-methylimidazole, pure water and absolute ethyl alcohol are mixed according to the mass ratio of 1:4:10:100, uniformly mixing, stirring and reacting for 5 hours at 30 ℃ and 300r/min, centrifugally separating, washing for 3 times by using absolute ethyl alcohol, and drying for 8 hours at 10 ℃ and 1Pa to prepare a metal organic framework; preheating a metal organic framework at 100 ℃ for 40min in a nitrogen atmosphere, heating to 250 ℃ at the speed of 5 ℃/min, standing for 80min, heating to 800 ℃, and standing for 3h to obtain nitrogen-containing carbon quantum dots; adding nitrogen-containing carbon quantum dots with the mass 0.03 times of that of the anaerobic treatment liquid into the anaerobic treatment liquid, stirring for 50min at 30 ℃ and 300r/min, filtering, and drying for 8h at 10 ℃ and 1Pa to prepare active granular sludge;
(4) And (3) proliferation culture: placing the activated granular sludge in a liquid culture medium with the mass 20 times that of the activated granular sludge, standing for 36h in an anaerobic environment at 30 ℃, filtering, and drying for 8h at 10 ℃ under 1Pa to prepare anaerobic granular sludge for papermaking wastewater; the liquid culture medium solvent is water, and comprises 20% of polyvalent peptone, 1% of tryptone, 0.5% of yeast extract, 0.5% of sodium chloride, 5% of beef extract, 0.04% of cysteine and 0.5% of hemin.
Example 2
A proliferation and bacterium cultivation method of anaerobic granular sludge for papermaking wastewater mainly comprises the following preparation steps:
(1) Flocculation treatment: adding a polyacrylamide flocculant into the papermaking wastewater until no obvious floccule is generated, removing the floccule, and naturally standing for 22 hours to obtain flocculated papermaking wastewater;
(2) Anaerobic treatment: adding nutrient solution which is 0.15 times of the mass of the flocculated papermaking wastewater into the flocculated papermaking wastewater, sealing the flocculated papermaking wastewater by using a polyethylene film, and standing the flocculated papermaking wastewater for 33 hours at 35 ℃ to obtain anaerobic treatment solution; the solvent of the nutrient solution is water, and the nutrient solution comprises 25g/L glucose, 8g/L magnesium sulfate, 7g/L calcium chloride, 1.5g/L cobalt chloride, 1.5g/L ferric chloride, 0.04g/L zinc chloride, 0.09 g/sodium chloride, 0.04g/L nickel chloride and 0.25mL/L hydrogen chloride;
(3) Granulating: zinc nitrate, 2-methylimidazole, pure water and absolute ethyl alcohol are mixed according to the mass ratio of 1:4.5:15:110, stirring and reacting for 4 hours at 35 ℃ and 400r/min, centrifugally separating, washing for 4 times by using absolute ethyl alcohol, and drying for 7 hours at 15 ℃ and 5Pa to prepare a metal organic framework; preheating a metal organic framework at 110 ℃ for 35min in a nitrogen atmosphere, heating to 280 ℃ at the speed of 5.5 ℃/min, standing for 70min, heating to 850 ℃, and standing for 2.5h to obtain nitrogen-containing carbon quantum dots; adding nitrogen-containing carbon quantum dots with the mass 0.04 times of that of the anaerobic treatment liquid into the anaerobic treatment liquid, stirring for 45min at 35 ℃ and 400r/min, filtering, and drying for 7h at 15 ℃ and 5Pa to obtain active granular sludge;
(4) And (3) proliferation culture: placing the activated granular sludge in a liquid culture medium with the mass of 25 times that of the activated granular sludge, standing for 33h at 35 ℃ in an anaerobic environment, filtering, and drying for 7h at 15 ℃ under 5Pa to prepare anaerobic granular sludge for papermaking wastewater; the liquid culture medium solvent is water, and comprises 20% of polyvalent peptone, 1% of tryptone, 0.5% of yeast extract, 0.5% of sodium chloride, 5% of beef extract, 0.04% of cysteine and 0.5% of hemin.
Example 3
A proliferation and bacterium cultivation method of anaerobic granular sludge for papermaking wastewater mainly comprises the following preparation steps:
(1) Flocculation treatment: adding a polyacrylamide flocculant into the papermaking wastewater until no obvious floccule is generated, removing the floccule, naturally standing for 24 hours to obtain flocculated papermaking wastewater;
(2) Anaerobic treatment: adding nutrient solution which is 0.2 time of the mass of the flocculated papermaking wastewater into the flocculated papermaking wastewater, sealing the flocculated papermaking wastewater by using a polyethylene film, and standing the flocculated papermaking wastewater for 30 hours at 40 ℃ to obtain anaerobic treatment solution; the solvent of the nutrient solution is water and comprises 30g/L glucose, 9g/L magnesium sulfate, 8g/L calcium chloride, 2g/L cobalt chloride, 2g/L ferric chloride, 0.05g/L zinc chloride, 0.1 g/L sodium chloride, 0.05g/L nickel chloride and 0.3mL/L hydrogen chloride;
(3) Granulating: zinc nitrate, 2-methylimidazole, pure water and absolute ethyl alcohol are mixed according to the mass ratio of 1:5:20:120, stirring and reacting for 3 hours at 40 ℃ and 500r/min, centrifugally separating, washing for 5 times by using absolute ethyl alcohol, and drying for 6 hours at 20 ℃ and 10Pa to obtain a metal organic framework; preheating a metal organic framework at 120 ℃ for 30min in a nitrogen atmosphere, heating to 300 ℃ at the speed of 6 ℃/min, standing for 60min, heating to 900 ℃, and standing for 2h to obtain nitrogen-containing carbon quantum dots; adding nitrogen-containing carbon quantum dots with the mass 0.05 time of that of the anaerobic treatment liquid into the anaerobic treatment liquid, stirring for 40min at 40 ℃ and 500r/min, filtering, and drying for 6h at 20 ℃ and 10Pa to obtain active granular sludge;
(4) And (3) proliferation culture: placing the activated granular sludge in a liquid culture medium with the mass 30 times that of the activated granular sludge, standing for 30h at 40 ℃ in an anaerobic environment, filtering, and drying for 6h at 20 ℃ under 10Pa to prepare anaerobic granular sludge for papermaking wastewater; the liquid culture medium solvent is water, and comprises 20% of polyvalent peptone, 1% of tryptone, 0.5% of yeast extract, 0.5% of sodium chloride, 5% of beef extract, 0.04% of cysteine and 0.5% of hemin.
Comparative example 1
A proliferation and bacterium culture method of anaerobic granular sludge for papermaking wastewater mainly comprises the following preparation steps:
(1) Flocculation treatment: adding a polyacrylamide flocculant into the papermaking wastewater until no obvious floccule is generated, removing the floccule, naturally standing for 22h to obtain flocculated papermaking wastewater;
(2) Anaerobic treatment: adding nutrient solution which is 0.15 times of the mass of the flocculated papermaking wastewater into the flocculated papermaking wastewater, sealing the flocculated papermaking wastewater by using a polyethylene film, and standing the flocculated papermaking wastewater for 33 hours at 35 ℃ to obtain anaerobic treatment solution; the solvent of the nutrient solution is water, and the nutrient solution comprises 25g/L glucose, 8g/L magnesium sulfate, 7g/L calcium chloride, 1.5g/L cobalt chloride, 1.5g/L ferric chloride, 0.04g/L zinc chloride, 0.09 g/L sodium chloride, 0.04g/L nickel chloride and 0.25mL/L hydrogen chloride;
(3) Granulating: zinc nitrate, terephthalic acid, pure water and absolute ethyl alcohol are mixed according to the mass ratio of 1:4.5:15:110, stirring and reacting for 4 hours at 35 ℃ and 400r/min, centrifugally separating, washing for 4 times by using absolute ethyl alcohol, and drying for 7 hours at 15 ℃ and 5Pa to prepare a metal organic framework; preheating a metal organic framework at 110 ℃ for 35min in a nitrogen atmosphere, heating to 280 ℃ at the speed of 5.5 ℃/min, standing for 70min, heating to 850 ℃, and standing for 2.5h to obtain nitrogen-containing carbon quantum dots; adding carbon quantum dots with the mass 0.04 time of that of the anaerobic treatment liquid into the anaerobic treatment liquid, stirring for 45min at 35 ℃ and 400r/min, filtering, and drying for 7h at 15 ℃ and 5Pa to obtain activated granular sludge;
(4) And (3) proliferation culture: placing the activated granular sludge in a liquid culture medium with the mass of 25 times that of the activated granular sludge, standing for 33h at 35 ℃ in an anaerobic environment, filtering, and drying for 7h at 15 ℃ under 5Pa to prepare anaerobic granular sludge for papermaking wastewater; the liquid culture medium solvent is water, and comprises 20% of polyvalent peptone, 1% of tryptone, 0.5% of yeast extract, 0.5% of sodium chloride, 5% of beef extract, 0.04% of cysteine and 0.5% of hemin.
Examples of effects
The following table 2 shows the results of performance analysis of the decontamination performance of the anaerobic granular sludge for papermaking wastewater prepared by the proliferation culture method of the anaerobic granular sludge for papermaking wastewater in examples 1 to 3 of the present invention and in comparative example 1:
| rate of decrease in absorption | Rate of decrease in absorption | ||
| Example 1 | 18.5% | Example 3 | 18.9% |
| Example 2 | 18.8% | Comparative example 1 | 7.2% |
TABLE 1
From the comparison of the experimental data of examples 1, 2 and 3 and comparative example 1 in table 1, it can be seen that the light absorption reduction rate of examples 1, 2 and 3 is high compared with that of comparative example 1, which indicates that the nitrogen-containing carbon quantum dots obtained by carbonizing the zinc nitrate and 2-methylimidazole after reaction have a better adsorption loading effect on microorganisms in the water body and allow the microorganisms to proliferate on the nitrogen-containing carbon quantum dots, so that the anaerobic granular sludge for papermaking wastewater has a good decontamination effect.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (7)
1. A proliferation and bacterium cultivation method of anaerobic granular sludge for papermaking wastewater mainly comprises the following preparation steps: flocculation treatment, anaerobic treatment, granulation and proliferation culture;
mainly comprises the following preparation steps:
(1) Flocculation treatment: adding a polyacrylamide flocculant into the papermaking wastewater until no obvious floccule is generated, removing the floccule, naturally standing for 20 to 24h to obtain flocculated papermaking wastewater;
(2) Anaerobic treatment: adding a nutrient solution with the mass of 0.1 to 0.2 times of that of the flocculated papermaking wastewater into the flocculated papermaking wastewater, sealing the mixture by using a polyethylene film, and standing the mixture for 30 to 36h at the temperature of 30 to 40 ℃ to obtain an anaerobic treatment solution;
(3) Granulating: adding nitrogen-containing carbon quantum dots with the mass 0.03 to 0.05 times of that of the anaerobic treatment liquid into the anaerobic treatment liquid, stirring at 30 to 40 ℃ and 300 to 500r/min for 40 to 50min, filtering, and drying at 10 to 20 ℃ and 1 to 10Pa for 6 to 8h to prepare active granular sludge;
(4) And (3) proliferation culture: placing the activated granular sludge in a liquid culture medium with the mass of 20-30 times that of the activated granular sludge, standing for 30-36h in an anaerobic environment at 30-40 ℃, filtering, and drying for 6-8h at 10-20 ℃ and 1-10Pa to prepare the anaerobic granular sludge for the papermaking wastewater.
2. The method for proliferating and cultivating the anaerobic granular sludge for the papermaking wastewater as claimed in claim 1, wherein the papermaking wastewater in the step (1) is one or more of waste liquor generated by cooking wood pulp, wastewater discharged from a pulping machine and a refiner and wastewater discharged from a papermaking machine.
3. The method for proliferating the anaerobic granular sludge for papermaking wastewater according to claim 2, wherein a solvent of the nutrient solution in the step (2) is water, and the components of the solvent include 20 to 30g/L glucose, 7 to 9g/L magnesium sulfate, 6 to 8g/L calcium chloride, 1 to 2g/L cobalt chloride, 1 to 2g/L ferric chloride, 0.03 to 0.05g/L zinc chloride, 0.08 to 0.1g/L sodium chloride, 0.03 to 0.05g/L nickel chloride and 0.2 to 0.3mL/L hydrogen chloride.
4. The proliferation and cultivation method of anaerobic granular sludge for papermaking wastewater according to claim 3, wherein the preparation method of the nitrogen-containing carbon quantum dots in the step (3) comprises the following steps: preheating a metal organic framework at 100 to 120 ℃ for 30 to 40min in a nitrogen atmosphere, raising the temperature to 250 to 300 ℃ at the speed of 5 to 6 ℃/min, standing for 60 to 80min, raising the temperature to 800 to 900 ℃, and standing for 2 to 3h to prepare the nitrogen-containing carbon quantum dot.
5. The proliferation and cultivation method of anaerobic granular sludge for papermaking wastewater according to claim 4, characterized in that the preparation method of the metal organic framework comprises the following steps: zinc nitrate, 2-methylimidazole, pure water and absolute ethyl alcohol are mixed according to the mass ratio of 1:4:10:100 to 1:5:20:120, stirring and reacting for 3 to 5 hours at the temperature of 30 to 40 ℃ and at the speed of 300 to 500r/min, centrifugally separating, washing for 3 to 5 times by using absolute ethyl alcohol, and drying for 6 to 8 hours at the temperature of 10 to 20 ℃ and at the pressure of 1 to 10Pa to prepare the nano-composite material.
6. The method for proliferating and cultivating the anaerobic granular sludge for the paper-making wastewater according to claim 5, wherein the liquid culture medium solvent in the step (4) is water, and the composition of the liquid culture medium solvent comprises 20% of polypeptone, 1% of tryptone, 0.5% of yeast extract, 0.5% of sodium chloride, 5% of beef extract, 0.04% of cysteine and 0.5% of hemin.
7. The method for proliferating and cultivating the anaerobic granular sludge for the papermaking wastewater according to claim 6, wherein the anaerobic granular sludge for the papermaking wastewater, which is prepared by the method for proliferating and cultivating the anaerobic granular sludge for the papermaking wastewater, is applied to pollution treatment of waste liquor generated by cooking wood pulp, waste water discharged from a pulping machine and a refiner and paper machine waste water.
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