CN115253090A - Photodynamic therapy equipment at somatic cell level - Google Patents
Photodynamic therapy equipment at somatic cell level Download PDFInfo
- Publication number
- CN115253090A CN115253090A CN202211205896.8A CN202211205896A CN115253090A CN 115253090 A CN115253090 A CN 115253090A CN 202211205896 A CN202211205896 A CN 202211205896A CN 115253090 A CN115253090 A CN 115253090A
- Authority
- CN
- China
- Prior art keywords
- light
- sample
- scanning
- signal
- axis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002428 photodynamic therapy Methods 0.000 title claims abstract description 47
- 210000001082 somatic cell Anatomy 0.000 title claims abstract description 10
- 210000004027 cell Anatomy 0.000 claims abstract description 51
- 230000003044 adaptive effect Effects 0.000 claims abstract description 36
- 230000001225 therapeutic effect Effects 0.000 claims abstract description 31
- 238000011282 treatment Methods 0.000 claims abstract description 19
- 238000003384 imaging method Methods 0.000 claims abstract description 10
- 230000001678 irradiating effect Effects 0.000 claims abstract description 8
- 238000000034 method Methods 0.000 claims description 24
- 230000003287 optical effect Effects 0.000 claims description 21
- 230000008569 process Effects 0.000 claims description 15
- 201000010099 disease Diseases 0.000 claims description 13
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 13
- 230000005540 biological transmission Effects 0.000 claims description 9
- 230000001413 cellular effect Effects 0.000 claims description 4
- 230000000638 stimulation Effects 0.000 claims description 2
- 238000003325 tomography Methods 0.000 claims description 2
- 230000000392 somatic effect Effects 0.000 claims 5
- 231100000915 pathological change Toxicity 0.000 abstract description 13
- 230000036285 pathological change Effects 0.000 abstract description 13
- 230000006378 damage Effects 0.000 abstract description 5
- 238000012014 optical coherence tomography Methods 0.000 description 34
- 238000005516 engineering process Methods 0.000 description 14
- 206010028980 Neoplasm Diseases 0.000 description 7
- 230000003902 lesion Effects 0.000 description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 229910052760 oxygen Inorganic materials 0.000 description 6
- 239000001301 oxygen Substances 0.000 description 6
- 239000003504 photosensitizing agent Substances 0.000 description 6
- 230000001427 coherent effect Effects 0.000 description 3
- 239000003642 reactive oxygen metabolite Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 206010005003 Bladder cancer Diseases 0.000 description 2
- 208000003174 Brain Neoplasms Diseases 0.000 description 2
- 208000000461 Esophageal Neoplasms Diseases 0.000 description 2
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 2
- 206010030155 Oesophageal carcinoma Diseases 0.000 description 2
- 206010034972 Photosensitivity reaction Diseases 0.000 description 2
- 241001303601 Rosacea Species 0.000 description 2
- 208000007097 Urinary Bladder Neoplasms Diseases 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 230000030833 cell death Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 201000004101 esophageal cancer Diseases 0.000 description 2
- 201000005202 lung cancer Diseases 0.000 description 2
- 208000020816 lung neoplasm Diseases 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000036211 photosensitivity Effects 0.000 description 2
- 201000004700 rosacea Diseases 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 201000005112 urinary bladder cancer Diseases 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 208000024248 Vascular System injury Diseases 0.000 description 1
- 208000035868 Vascular inflammations Diseases 0.000 description 1
- 208000012339 Vascular injury Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 125000001475 halogen functional group Chemical group 0.000 description 1
- 230000013632 homeostatic process Effects 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000012634 optical imaging Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000002638 palliative care Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 208000017983 photosensitivity disease Diseases 0.000 description 1
- 231100000434 photosensitization Toxicity 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 238000001959 radiotherapy Methods 0.000 description 1
- 230000002207 retinal effect Effects 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N5/00—Radiation therapy
- A61N5/06—Radiation therapy using light
- A61N5/0613—Apparatus adapted for a specific treatment
- A61N5/062—Photodynamic therapy, i.e. excitation of an agent
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0062—Arrangements for scanning
- A61B5/0066—Optical coherence imaging
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N5/00—Radiation therapy
- A61N5/06—Radiation therapy using light
- A61N2005/063—Radiation therapy using light comprising light transmitting means, e.g. optical fibres
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- Radiology & Medical Imaging (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Heart & Thoracic Surgery (AREA)
- Surgery (AREA)
- Molecular Biology (AREA)
- Medical Informatics (AREA)
- Physics & Mathematics (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The application discloses a photodynamic therapy device at a somatic cell level, which is used for solving the technical problem that the precision of the existing photodynamic therapy is not enough to damage normal cells around pathological change tissues. The apparatus comprises: the photodynamic therapy system comprises a first light source and a focusing element, and the adaptive OCT system comprises a double-shaft scanning system, a wavefront corrector and a computer; the first light source is used for emitting a therapeutic light signal, and the adjustable focusing element is used for adjusting the focusing depth of the therapeutic light signal reaching the position to be treated of the sample under the control of the computer; the double-shaft scanning system is used for irradiating the treatment light signals to the position to be treated and scanning the sample through the self-adaptive OCT system to form three-dimensional image information of the position to be treated of the sample; the wave-front corrector is used for correcting the wave-front information of the therapeutic light signal in real time so as to focus the therapeutic light signal to a type of cells or a layer of cells at the position to be treated, and is used for enabling the OCT system to achieve cell-level imaging.
Description
Technical Field
The application relates to the technical field of optical systems, in particular to photodynamic therapy equipment at a somatic cell level.
Background
Cancer is one of public health diseases in the world, is difficult to cure, has high death rate, and affects the life quality and survival rate of patients all over the world. In recent years, photodynamic therapy has been widely used in the field of treatment of other tumors such as lung cancer, bladder cancer, esophageal cancer, and brain cancer. Photodynamic therapy is a technique for diagnosing and treating diseases by utilizing the photodynamic effect, and the basis of the photodynamic effect is the photodynamic effect. The photodynamic effect is a photosensitization reaction accompanied with biological effect and involving oxygen molecules, the process is that a photosensitizer absorbed by tissues is excited by laser irradiation with specific wavelength, the excited photosensitizer transfers energy to surrounding oxygen to generate active oxygen substances with strong activity, such as singlet oxygen, hydroxyl free radicals and the like, and the active oxygen substances and adjacent biological macromolecules undergo oxidation reaction to further cause cell damage and death.
The active oxygen species include H2O2, O2-,. OH, 1O2, and the like. For normal living tissue, the reactive oxygen species is a pair of double-edged sword. The active oxygen substance with proper concentration is beneficial to cell proliferation and maintaining homeostasis, and can make the cell not invaded by other organisms by modifying protein structure. In cells, there is an antioxidant system that regulates the intracellular reactive oxygen species to maintain a suitable concentration. Once the balance of production and elimination of reactive oxygen species is broken, cellular components are oxidized, thereby triggering cell death, vascular injury, and inflammation and immune responses, ultimately eliminating the tumor, which is also the underlying mechanism by which photodynamic therapy kills cells.
In recent years, photodynamic therapy has been widely used in the field of treatment of other tumors such as lung cancer, bladder cancer, esophageal cancer, and brain cancer. Compared with conventional treatment means such as operation, chemotherapy, radiotherapy and the like, the photodynamic therapy has the advantages of small wound, low toxicity, good selectivity, good applicability, repeated treatment, palliative treatment, cooperative operation for improving the curative effect, elimination of hidden cancer focuses, protection of the appearance and important organ functions, short treatment time and the like. Furthermore, photodynamic therapy has also been successfully applied in different fields of non-malignant diseases, such as urology, immunology, ophthalmology, dentistry, dermatology, etc.
Optical Coherence Tomography (OCT) is an emerging Optical imaging technique. The method utilizes the basic principle of a weak coherent light interferometer to detect back reflection or several scattering signals of incident weak coherent light at different depth levels of the biological tissue, and a two-dimensional structural image of the biological tissue can be obtained by scanning. The OCT technology has become a breakthrough technology after X-CT and MRI technologies due to its characteristics of real-time, in vivo, non-invasive, high resolution, low cost, etc. Nowadays, the OCT technology has been applied to clinical diagnosis in ophthalmology, dentistry, and dermatology, and has a wide application prospect in biological tissue biopsy and imaging.
Light from a light source in an OCT system is split into two beams, one beam entering the reference arm and one beam entering the sample arm. The light entering the sample arm is irradiated on the sample, and the light can obtain a cross-sectional image of the sample to be detected only by penetrating the upper layer of the sample to be detected to reach the deep structure. When light reaches the deep position, the light intensity is attenuated to a certain degree, the light attenuation can limit the imaging depth of the OCT technology, the integral color sense of an OCT image of the detected sample is inconsistent, and the local contrast at the deep position is seriously reduced, so that the deep structure information of the detected sample is covered. Ideally, the beam emitted by the OCT system can be focused on a certain point of the sample without any influence. However, in actual transmission, turbulence, thermal bloom causes small changes in the light, resulting in distortion of the transmitted wavefront, and hence divergence of the beam, reducing the intensity of the beam delivered to this point.
Adaptive Optics (AO) is a technique for improving the performance of an optical system by correcting the distortion of the wavefront of a light wave caused by turbulence and thermal halo using deformable mirrors. Adaptive optics technology was initially applied in the astronomical field, adaptive optics was applied to vision studies until Junzhong Liang et al, the university of rochester vision science center, 1994, introduced hartmann cameras and adaptive optics technology into retinal imaging, the first adaptive optics imaging system for the human eye in the world was established, and high resolution single cell (cone) images of living human eyes were obtained for the first time. In 2005, the university of indiana optometry college, jungtae Rha et al, introduced an adaptive optics system into a spectral optical coherence tomography, greatly improved the imaging resolution of SD-OCT, and obtained clear images of individual cone-shaped cells in the 1 ° and 2.4 ° regions of the fundus fovea, respectively. Adaptive imaging techniques have been developed rapidly in recent years, because adaptive optics techniques can correct the phase difference of the human eye in real time to obtain images close to the diffraction limit level.
However, the above prior art has the following problems: 1. the three-dimensional imaging of the lesion tissue cannot be performed, and the change condition of the lesion tissue in the treatment process cannot be observed. 2. Light cannot be precisely irradiated to the diseased cell layer. 3. The accuracy of photodynamic therapy is not sufficient. The light in the existing photodynamic therapy can generate a divergence phenomenon when being irradiated to a sample, and can not focus the light on a single cell, thereby causing certain damage to normal cells around a pathological change tissue. 4. The photodynamic technology uses the photosensitivity of a photosensitizer, the photosensitizer needs to act on tissue cells, and certain influence is caused on normal cells around pathological change tissues, and light in the existing photodynamic therapy can generate a divergence phenomenon when being irradiated to the pathological change tissues and also can cause certain damage to the normal cells around the pathological change tissues.
Disclosure of Invention
The embodiment of the application provides a photodynamic therapy device at a somatic cell level, which is used for solving the technical problem that the conventional photodynamic therapy has insufficient precision and can damage normal cells around pathological change tissues.
Embodiments provide a photodynamic therapy device at a somatic cell level, the device comprising: an adaptive Optics Coherence Tomography (OCT) system for generating three-dimensional images at the cellular level of a sample, and a photodynamic therapy system for therapeutic light stimulation of a location to be treated on the sample; wherein the adaptive OCT system comprises a dual-axis scanning system, a wavefront corrector and a computer, the photodynamic therapy system comprises a first light source and a focusing element; the first light source is used for emitting a therapeutic light signal, and the adjustable focusing element is used for adjusting the focusing depth of the therapeutic light signal to the position to be treated of the sample under the control of the computer; the double-axis scanning system is used for irradiating the treatment light signals to the position to be treated and scanning a sample through the self-adaptive OCT system to form three-dimensional image information of the position to be treated; the wave-front corrector is used for correcting the wave-front information of the therapeutic light signal in real time so as to focus the therapeutic light signal to a type of cells or a layer of cells at the position to be treated, and is used for enabling the OCT system to achieve cell-level imaging.
In one or more embodiments of the present disclosure, the computer is configured to observe and measure the size, shape and number of nuclei of cells in the sample according to the generated three-dimensional image at the cell level, to determine disease information corresponding to a position to be treated of the sample, and to determine treatment light signal information corresponding to the sample based on the disease information.
In one or more embodiments of the present specification, the adaptive OCT system further includes: the second light source, the first coupler, the reflector and the second coupler; the double-axis scanning system comprises a first light source, a first coupler, a reflector, a second light source, a second coupler and a computer, wherein the first light source is used for emitting scanning light signals, the first coupler is used for dividing the scanning light signals into a first beam of light and a second beam of light, the double-axis scanning system is further used for scanning the position to be treated of a sample through the first beam of light to generate backscattered light with sample information, the reflector is used for reflecting the second beam of light to generate reflected light, the second coupler is used for receiving the backscattered light with the sample information and the reflected light and generating interference light signals based on the backscattered light with the sample information and the reflected light, and the computer is further used for generating the cell-level three-dimensional image based on the interference light signals.
In one or more embodiments of the present specification, the adaptive OCT system further includes: a first circulator and a second circulator; the first circulator is used for providing an optical transmission channel for the second beam of light and the reflected light; the second circulator is used for providing an optical transmission channel for the first light and the backscattered light with the sample information.
In one or more embodiments of the present description, the adaptive OCT system further includes: a first collimator and a second collimator; the first collimator is used for adjusting the second beam of light into a parallel beam of light; the second collimator is used for adjusting the first light beam into a parallel light beam.
In one or more embodiments of the present description, the photodynamic therapy system further comprises: a dichroic mirror; the dichroic mirror is used for receiving the parallel light beams corresponding to the first light beam and sending all the parallel light beams corresponding to the first light beam into the double-axis scanning system; and a dual-axis scanning system for reflecting the focused therapeutic light signal and sending the focused therapeutic light signal to the dual-axis scanning system; and the device is also used for receiving an optical signal returned after the biaxial scanning system irradiates the sample, wherein the optical signal returned by the sample contains the backscattered light with the sample information.
In one or more embodiments of the present description, the adaptive OCT system further includes: a signal receiving device; the signal receiving device is used for receiving the interference optical signal generated by the second coupler and converting the interference optical signal into a corresponding electrical signal.
In one or more embodiments of the present description, the adaptive OCT system further includes: a signal acquisition device; the signal acquisition device is used for acquiring the electric signal transmitted by the signal receiving device and transmitting the electric signal to a computer, and the computer is used for converting the electric signal into a digital signal and generating the cell-level three-dimensional image according to the digital signal.
In one or more embodiments of the present description, the adaptive OCT system further includes: a scanning lens; the scanning lens is used for irradiating the first beam of light transmitted by the double-axis scanning system to the sample and receiving backscattered light with sample information generated after the first beam of light is irradiated to the sample.
In one or more embodiments of the present disclosure, the dual-axis scanning system is configured to scan the sample after the first beam of light is irradiated to the sample through the scanning lens, specifically: the biaxial scanning system is X, Y and is used for focusing the first beam of light on the sample through the scanning lens, and after a Y axis of the biaxial scanning system enters a first preset scanning position, an X axis of the biaxial scanning system moves transversely to complete scanning of a first preset scanning area of the sample; then, the double-shaft scanning system controls the Y shaft to move longitudinally to a second preset scanning position of the sample, and the X shaft of the double-shaft scanning system moves transversely after entering the second preset scanning position, so that the scanning of a second preset scanning area of the sample is completed; repeatedly executing the process until the Y axis of the double-axis scanning system longitudinally moves to the Nth preset scanning position of the sample, and then the X axis of the double-axis scanning system transversely moves to complete the scanning of the Nth preset scanning area of the sample; the first preset scanning position is arranged at the first end of the first preset scanning area, the second preset scanning position is arranged at the first end of the second preset scanning area, and the Nth preset scanning position is arranged at the first end of the Nth preset scanning area.
The photodynamic therapy equipment at the level of the somatic cells provided by the embodiment of the application has the following beneficial effects: by combining the adaptive OCT system with the photodynamic therapy system, the cell-level three-dimensional structure of the pathological change tissue can be observed, then light with different wavelengths is selected to treat the pathological change cells according to the condition of the pathological change tissue, and the cell-level disease treatment is realized.
Drawings
The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the application and together with the description serve to explain the application and not to limit the application. In the drawings:
fig. 1 is a schematic diagram of an architecture of a photodynamic therapy device at a somatic cell level according to an embodiment of the present application.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, the technical solutions of the present application will be described in detail and completely with reference to the following specific embodiments of the present application and the accompanying drawings. It should be apparent that the described embodiments are only some of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
The photodynamic therapy equipment provided by the embodiment of the application combines the self-adaptive OCT technology and the photodynamic technology, and the self-adaptive OCT technology can detect and analyze the internal structure of the sample tissue to obtain cell information. The photodynamic technology uses the photosensitivity of a photosensitizer, and light acts on pathological change tissues to carry out targeted treatment, kill pathological change cells and achieve the purpose of treatment. The self-adaptive OCT technology is combined with the photodynamic technology, so that light can directly act on cells of a pathological change part without using a photosensitizer, and normal cells around a pathological change tissue are protected to the maximum extent.
The technical solutions proposed in the embodiments of the present application are described in detail below with reference to the accompanying drawings.
Fig. 1 is a schematic diagram of an architecture of a photodynamic therapy device at a somatic cell level according to an embodiment of the present application. As shown in fig. 1, the photodynamic therapy device in the embodiment of the present application includes: the device comprises a second light source 1, a first coupler 2, a first circulator 3, a first collimator 4, a reflecting mirror 5, a second circulator 6, a second collimator 7, a dichroic mirror 8, a dual-axis scanning system 9, a wavefront corrector 10, a scanning lens 11, a second coupler 13, a signal receiving device 14, a signal acquisition device 15, a computer 16, a first light source 17 and a focusable element 18.
To illustrate the principle of the optical path system of the combination of the adaptive OCT system and the photodynamic therapy system, the simplest adaptive OCT system is taken as an example, and the adaptive OCT system used in the practical application includes, but is not limited to, the optical path described in the embodiment of the present application.
In one or more possible implementations of embodiments of the present application, the photodynamic therapy device comprises two subsystems consisting of the above-described devices: adaptive OCT systems, photodynamic therapy systems; the adaptive OCT system is used for generating a cell-level three-dimensional image of a sample, and the photodynamic therapy system is used for performing therapeutic photostimulation on a position to be treated on the sample.
Further, the adaptive OCT system includes: a second light source 1, a first coupler 2, a first circulator 3, a first collimator 4, a reflector 5, a second circulator 6, a second collimator 7, a dichroic mirror 8, a biaxial scanning system 9, a wavefront corrector 10, a scanning lens 11, a second coupler 13, a signal receiving device 14, a signal collecting device 15, and a computer 16, wherein the second light source 1 is configured to emit a scanning light signal, the first coupler 2 is configured to divide the scanning light signal into a first light and a second light (for convenience of description, the first light travels along an optical path corresponding to the second circulator 6, and the second light travels along an optical path corresponding to the first circulator 3), the first circulator 3 is configured to provide a light transmission channel for the second light, the first collimator 4 is configured to condition the second light into parallel light beams, the reflector 5 is configured to reflect the parallel light beams to generate reflected light, the second circulator 6 is configured to provide a light transmission channel for the first light, the second collimator 7 is used for adjusting the first light into a parallel light beam, the dichroic mirror 8 is used for sending the parallel light beam into the dual-axis scanning system 9, the dual-axis scanning system 9 transmits the light to the scanning lens 11, the first light irradiates the sample 12 through the scanning lens 11 to generate the back scattered light with the sample information, in addition, the wavefront corrector 10 is used for correcting the wavefront information of the first light in real time to improve the optical resolution, the second coupler 13 is used for receiving the back scattered light and the reflected light with the sample information and generating an interference light signal based on the back scattered light and the reflected light with the sample information, the signal receiving device 14 is used for receiving the interference light signal generated by the second coupler 13 and converting the interference light signal into a corresponding electrical signal, the signal collecting device 15 is used for collecting an electrical signal corresponding to the interference light signal, and transmitting the signal to a computer 16, wherein the computer 16 is used for converting the electric signal corresponding to the interference light signal into a digital signal and generating a cell-level three-dimensional image of the sample according to the digital signal.
In the above process, the first circulator 3 is also used to provide a light transmission channel for the reflected light of the second light, and the second circulator 6 is also used to provide a light transmission channel for the backscattered light with the sample information.
Still further, a photodynamic therapy system comprises: a first light source 17 and an adjustable focusing element 18, wherein the first light source 17 is used for emitting a therapeutic light signal, the adjustable focusing element 18 is used for adjusting the focusing depth of the therapeutic light signal under the control of the computer 16, the focused therapeutic light signal is focused to the position to be treated of the sample under the correction of the wavefront corrector, the position to be treated is determined by the computer 16 according to the cell-level three-dimensional image, and the wavelength of the therapeutic light signal is determined by the computer 16 according to the disease information corresponding to the position to be treated of the sample. In one example of the present application, the aforementioned site to be treated is a type of cell or a layer of cells of the sample 12.
In one or more possible implementations of the embodiments of the present application, the photodynamic therapy system further uses a dual-axis scanning system 9 and a wavefront corrector 10 in the adaptive OCT system, where the dual-axis scanning system 9 is used for transmitting an optical signal for treating a to-be-treated position of the sample 12, and the wavefront corrector 10 is used for correcting wavefront information of the treatment optical signal, so that the treatment optical signal acts on a type of cell or a layer of cells of the sample 12.
In the above process, the dichroic mirror 8 needs to send the therapeutic light signal into the biaxial scanning system 9, and also needs to receive the light signal returned after the light irradiates the sample, the returned light signal contains the backscattered light with the sample information, and the backscattered light with the sample information enters the first collimator 4 through the dichroic mirror 8.
In one or more possible implementations of the embodiment of the present application, the dual-axis scanning system 9 scans the sample 12 by irradiating a first beam of light through the scanning lens 11, specifically: the biaxial scanning system 9 is a X, Y biaxial scanning system, a first beam of light is focused on the sample 12 through the scanning lens 11, and after the Y axis of the biaxial scanning system 9 enters a first preset scanning position, the X axis of the biaxial scanning system 9 moves transversely to complete the scanning process of a first preset scanning area of the sample 12; then, the double-axis scanning system 9 controls the Y axis to move longitudinally to a second preset scanning position of the sample 12, and after entering the second preset scanning position, the X axis of the double-axis scanning system 9 moves transversely, so as to complete the scanning process of the second preset scanning area of the sample 12; repeating the above process until the Y axis is controlled by the dual-axis scanning system 9 to move longitudinally to the Nth preset scanning position of the sample 12, and then the X axis of the dual-axis scanning system 9 moves transversely to complete the scanning process of the Nth preset scanning area of the sample 12; the first preset scanning position is at the first end of the first preset scanning area, the second preset scanning position is at the first end of the second preset scanning area, … …, the nth preset scanning position is at the first end of the nth preset scanning area, it should be noted that the first preset scanning position, the second preset scanning position, … …, and the nth preset scanning position are at the same end of the preset scanning area, and the first preset scanning area, the second preset scanning area, and the nth preset scanning area constitute the scanning area of the sample 12.
In one or more possible implementations of the embodiments of the present application, the computer 16 determines the treatment light signal information corresponding to the sample 12 based on the disease information, specifically, the computer 16 determines the treatment light signal to be 630nm red light when the disease is a tumor; and, when the disease is type II rosacea, the therapeutic light signal is determined to be 633nm red light, that is, 630nm red light for tumor therapy and 633nm red light for type II rosacea therapy, and the second light source 1 in the embodiment of the present application employs a near-infrared broad-spectrum weak coherent light source having a central wavelength of 1050nm and a width wavelength range of 3dB in the range of 1000-1100nm.
The photodynamic therapy system in the embodiments of the present application is further described below by the propagation of light:
after being irradiated into the first coupler 2, the light of the second light source 1 of the adaptive OCT system is divided into two beams, namely a first beam and a second beam. The second light beam enters the first circulator 3 from the channel 22 of the first circulator 3 and then enters the first collimator 4 from the channel 24 of the first circulator 3, the first collimator 4 converts the light beam into a parallel beam and irradiates the light beam onto the reflector 5, the light beam irradiating the reflector 5 is reflected to generate reflected light, the reflected light enters the first circulator 4 from the channel 24 of the first circulator 3 through the first collimator 4, and then enters the second coupler 13 from the channel 23 of the first circulator 3 after running to the channel 23 of the first circulator 4 in the first circulator 4. The first light enters the second circulator 6 through a channel 19 of the second circulator 6, then enters a second collimator 7 through a channel 21 of the second circulator 6, the second collimator 7 converts the light into parallel light beams and irradiates the parallel light beams to the dichroic mirror 8, at this time, all the first light irradiating the dichroic mirror 8 enters the biaxial scanning system 9 through the dichroic mirror 8, the biaxial scanning system 9 transmits the first light to the wavefront corrector 10, the wavefront corrector 10 corrects wavefront information of the first light, and then transmits the corrected first light to the scanning lens 11 in sequence, and irradiates the sample 12 through the scanning lens 11 to obtain backscattered light with sample information, after the backscattered light with the sample information passes through the scanning lens 11, the wavefront corrector 10, the biaxial scanning system 9, the dichroic mirror 8 and the second collimator 7 in sequence, the backscattered light with the sample information enters the second coupler 13 through a channel 20 of the second circulator 6 through a channel 21 of the second circulator 6, and then interferes with the second light (i.e., the reflected light of the second light) of the reflecting mirror 5 in the second coupler 13 to form interference light, the reflected light is converted into an electrical signal corresponding to a digital signal acquisition device 14, and then is displayed on a computer 14 according to a digital signal acquisition device 14, and then is displayed on a computer 14.
In the above process, the computer 16 transmits a control command back to the wavefront corrector 10 according to an index such as image sharpness, and the wavefront corrector 10 corrects the wavefront information of the light transmitted from the dual-axis scanning system 9 according to the control command given by the computer 16.
Further, the first light source 17 of the photodynamic therapy system emits light with wavelength for treating corresponding disease, that is, a therapeutic light signal, the therapeutic light signal enters the focus-adjustable element 18 for focusing and then is transmitted to the dichroic mirror 8 of the adaptive OCT system, the dichroic mirror 8 reflects the therapeutic light signal, the reflected light enters the dual-axis scanning system 9, the dual-axis scanning system 9 emits the light, and then the light sequentially enters the wavefront corrector 10 and the scanning lens 11, and is irradiated to the sample 12 by the scanning lens 11.
In the foregoing process, the wavefront corrector 10, the first light source 17 and the adjustable focus element 18 are all controlled by the computer 16: the computer 16 controls the wavelength of the therapeutic light signal emitted by the first light source 17 to be suitable for treating the corresponding pathological cells; the focusing element 18 is controlled to adjust the focusing depth of the therapeutic light signal, the wavefront corrector 10 is controlled to correct the wavefront information of the received light, and the therapeutic light signal is focused on a certain type of cells or a certain layer of cells of the lesion.
In addition, the application method of the photodynamic therapy device in the embodiment of the application is as follows: the self-adaptive OCT system obtains a cell-level three-dimensional image of the scanning position of the sample 12, finds out the lesion position according to the cell-level three-dimensional image, gradually and accurately positions the scanning position to the lesion position, irradiates a treatment light signal emitted by the first light source 17 to the lesion position and kills lesion cells. The presence of diseased cells in the tissue of the sample 12 has a range and depth, and the illumination position and depth of focus of the first light source 17 is adjusted by the adjustable focus element 18 to reduce damage to normal tissue cells.
The embodiments in the present application are described in a progressive manner, and the same and similar parts among the embodiments can be referred to each other, and each embodiment focuses on the differences from the other embodiments.
It should also be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising a … …" does not exclude the presence of another identical element in a process, method, article, or apparatus that comprises the element.
The above description is only an example of the present application and is not intended to limit the present application. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the scope of the claims of the present application.
Claims (10)
1. Photodynamic therapy device at the level of somatic cells, characterized in that it comprises: an adaptive Optics Coherence Tomography (OCT) system for generating three-dimensional images at the cellular level of a sample, and a photodynamic therapy system for therapeutic light stimulation of a location to be treated on the sample; wherein,
the adaptive OCT system comprises a dual-axis scanning system, a wavefront corrector and a computer, wherein the photodynamic therapy system comprises a first light source and a focusing element;
the first light source is used for emitting a therapeutic light signal, and the adjustable focusing element is used for adjusting the focusing depth of the therapeutic light signal to the position to be treated of the sample under the control of the computer;
the double-axis scanning system is used for irradiating the treatment light signals to the position to be treated and scanning the position to be treated of the sample through the self-adaptive OCT system to form three-dimensional image information of the position to be treated of the sample;
the wavefront corrector is used for correcting the wavefront information of the therapeutic light signal in real time so as to enable the therapeutic light signal to be focused on a type of cells or a layer of cells at the position to be treated, and is used for improving the imaging resolution of the adaptive OCT system to obtain the cell-level three-dimensional image information of the position to be treated of the sample.
2. The apparatus of claim 1, wherein the computer is configured to observe and measure the size, shape and number of nuclei of the cells in the sample according to the generated three-dimensional image at the cellular level, to determine disease information corresponding to a location to be treated of the sample, and to determine treatment light signal information corresponding to the sample based on the disease information.
3. Photodynamic therapy device according to claim 1, characterized in that said adaptive OCT system further comprises: a second light source, a first coupler, a reflector, and a second coupler;
the double-axis scanning system comprises a first light source, a first coupler, a reflector, a second light source, a second coupler and a computer, wherein the first light source is used for emitting scanning light signals, the first coupler is used for dividing the scanning light signals into a first beam of light and a second beam of light, the double-axis scanning system is further used for scanning the position to be treated of a sample through the first beam of light to generate backscattered light with sample information, the reflector is used for reflecting the second beam of light to generate reflected light, the second coupler is used for receiving the backscattered light with the sample information and the reflected light and generating interference light signals based on the backscattered light with the sample information and the reflected light, and the computer is further used for generating the cell-level three-dimensional image based on the interference light signals.
4. Photodynamic therapy device at the somatic level according to claim 3, characterized in that the adaptive OCT system further comprises: a first circulator and a second circulator;
the first circulator is used for providing a light transmission channel for the second beam of light and the reflected light;
the second circulator is used for providing an optical transmission channel for the first light and the backscattered light with the sample information.
5. Photodynamic therapy device at the somatic level according to claim 3, characterized in that the adaptive OCT system further comprises: a first collimator and a second collimator;
the first collimator is used for adjusting the second beam of light into a parallel beam of light;
the second collimator is used for adjusting the first light beam into a parallel light beam.
6. Photodynamic therapy device according to claim 3, characterized in that said photodynamic therapy system further comprises: a dichroic mirror;
the dichroic mirror is used for receiving the parallel light beams corresponding to the first light beam and sending all the parallel light beams corresponding to the first light beam into the double-axis scanning system;
and a dual-axis scanning system for reflecting the focused therapeutic light signal and sending the focused therapeutic light signal to the dual-axis scanning system;
and the device is also used for receiving an optical signal returned after the biaxial scanning system irradiates the sample, wherein the optical signal returned by the sample contains the backscattered light with the sample information.
7. Photodynamic therapy device at the somatic level according to claim 3, characterized in that the adaptive OCT system further comprises: a signal receiving device;
the signal receiving device is used for receiving the interference optical signal generated by the second coupler and converting the interference optical signal into a corresponding electrical signal.
8. Photodynamic therapy device at the somatic level according to claim 7, characterized in that the adaptive OCT system further comprises: a signal acquisition device;
the signal acquisition device is used for acquiring the electric signal transmitted by the signal receiving device and transmitting the electric signal to a computer, and the computer is used for converting the electric signal into a digital signal and generating the cell-level three-dimensional image according to the digital signal.
9. Photodynamic therapy device at the somatic level according to claim 3, characterized in that the adaptive OCT system further comprises: a scanning lens;
the scanning lens is used for irradiating the first beam of light transmitted by the double-axis scanning system to the sample and receiving backscattered light with sample information generated after the first beam of light is irradiated to the sample.
10. The apparatus according to claim 9, wherein said dual-axis scanning system is configured to scan after said first beam of light is directed to said sample through said scanning lens, and comprises:
the biaxial scanning system is X, Y and is used for focusing the first beam of light on the sample through the scanning lens, and after a Y axis of the biaxial scanning system enters a first preset scanning position, an X axis of the biaxial scanning system moves transversely to complete scanning of a first preset scanning area of the sample;
then, the Y axis of the double-axis scanning system longitudinally moves to a second preset scanning position of the sample, and the X axis of the double-axis scanning system transversely moves to complete scanning of a second preset scanning area of the sample;
repeatedly executing the process until the Y axis of the double-axis scanning system longitudinally moves to the Nth preset scanning position of the sample, and then the X axis of the double-axis scanning system transversely moves to complete the scanning of the Nth preset scanning area of the sample;
the first preset scanning position is arranged at the first end of the first preset scanning area, the second preset scanning position is arranged at the first end of the second preset scanning area, and the Nth preset scanning position is arranged at the first end of the Nth preset scanning area.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211205896.8A CN115253090A (en) | 2022-09-30 | 2022-09-30 | Photodynamic therapy equipment at somatic cell level |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211205896.8A CN115253090A (en) | 2022-09-30 | 2022-09-30 | Photodynamic therapy equipment at somatic cell level |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115253090A true CN115253090A (en) | 2022-11-01 |
Family
ID=83757972
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211205896.8A Pending CN115253090A (en) | 2022-09-30 | 2022-09-30 | Photodynamic therapy equipment at somatic cell level |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115253090A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116548899A (en) * | 2023-04-19 | 2023-08-08 | 之江实验室 | Optical endoscopic diagnosis and treatment system and electronic device |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006005088A1 (en) * | 2004-07-13 | 2006-01-19 | Technische Universität Wien | Device for photodynamically treating diseases of the tissue and/or organs of living things |
| US20060058682A1 (en) * | 2002-06-12 | 2006-03-16 | Miller Donald T | Method and apparatus for improving both lateral and axial resolution in ophthalmoscopy |
| US20070073364A1 (en) * | 2005-09-29 | 2007-03-29 | Siemens Aktiengesellschaft | Combined OCT catheter device and method for combined optical coherence tomography (OCT) diagnosis and photodynamic therapy (PDT) |
| US20120330291A1 (en) * | 2011-06-24 | 2012-12-27 | The Regents Of The University Of California | Nonlinear optical photodynamic therapy (nlo-pdt) of the cornea |
| CN106455972A (en) * | 2014-04-28 | 2017-02-22 | 西北大学 | Apparatus, method and system for functional optical coherence tomography |
| CN108335732A (en) * | 2017-12-28 | 2018-07-27 | 西安中科微光影像技术有限公司 | A kind of the case recommendation method and its system of OCT images |
| RU187692U1 (en) * | 2018-12-13 | 2019-03-14 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Тамбовский государственный технический университет" (ФГБОУ ВО "ТГТУ") | Device for endoscopic optical coherence tomography with wavefront correction |
| CN110292359A (en) * | 2019-07-09 | 2019-10-01 | 浙江大学 | A method and device for label-free all-optical neuromodulation and imaging |
| CN110681070A (en) * | 2019-10-31 | 2020-01-14 | 福建师范大学 | A light source and control method for photodynamic therapy that can be individually regulated |
| CN110812704A (en) * | 2019-10-31 | 2020-02-21 | 中国医学科学院生物医学工程研究所 | Optical fiber adapter and OCT imaging system for in-vivo tissue photodynamic therapy |
| CN115024696A (en) * | 2022-08-12 | 2022-09-09 | 北京心联光电科技有限公司 | OCT imaging method, system and equipment |
-
2022
- 2022-09-30 CN CN202211205896.8A patent/CN115253090A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060058682A1 (en) * | 2002-06-12 | 2006-03-16 | Miller Donald T | Method and apparatus for improving both lateral and axial resolution in ophthalmoscopy |
| WO2006005088A1 (en) * | 2004-07-13 | 2006-01-19 | Technische Universität Wien | Device for photodynamically treating diseases of the tissue and/or organs of living things |
| US20070073364A1 (en) * | 2005-09-29 | 2007-03-29 | Siemens Aktiengesellschaft | Combined OCT catheter device and method for combined optical coherence tomography (OCT) diagnosis and photodynamic therapy (PDT) |
| US20120330291A1 (en) * | 2011-06-24 | 2012-12-27 | The Regents Of The University Of California | Nonlinear optical photodynamic therapy (nlo-pdt) of the cornea |
| CN106455972A (en) * | 2014-04-28 | 2017-02-22 | 西北大学 | Apparatus, method and system for functional optical coherence tomography |
| CN108335732A (en) * | 2017-12-28 | 2018-07-27 | 西安中科微光影像技术有限公司 | A kind of the case recommendation method and its system of OCT images |
| RU187692U1 (en) * | 2018-12-13 | 2019-03-14 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Тамбовский государственный технический университет" (ФГБОУ ВО "ТГТУ") | Device for endoscopic optical coherence tomography with wavefront correction |
| CN110292359A (en) * | 2019-07-09 | 2019-10-01 | 浙江大学 | A method and device for label-free all-optical neuromodulation and imaging |
| CN110681070A (en) * | 2019-10-31 | 2020-01-14 | 福建师范大学 | A light source and control method for photodynamic therapy that can be individually regulated |
| CN110812704A (en) * | 2019-10-31 | 2020-02-21 | 中国医学科学院生物医学工程研究所 | Optical fiber adapter and OCT imaging system for in-vivo tissue photodynamic therapy |
| CN115024696A (en) * | 2022-08-12 | 2022-09-09 | 北京心联光电科技有限公司 | OCT imaging method, system and equipment |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116548899A (en) * | 2023-04-19 | 2023-08-08 | 之江实验室 | Optical endoscopic diagnosis and treatment system and electronic device |
| CN116548899B (en) * | 2023-04-19 | 2024-02-06 | 之江实验室 | Optical endoscopic diagnosis and treatment systems and electronic devices |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5692988B2 (en) | Acoustic wave measuring device | |
| KR102117132B1 (en) | Dual modality imaging system for coregistered functional and anatomical mapping | |
| CA2788219C (en) | Optical coherence tomographic system for ophthalmic surgery | |
| Tian et al. | Novel photoacoustic microscopy and optical coherence tomography dual-modality chorioretinal imaging in living rabbit eyes | |
| CN104027068B (en) | A real-time multimodal photoacoustic human eye imaging system and its imaging method | |
| US20080119832A1 (en) | Multi-Modal Scanning Confocal Adaptive-Optic Macroscope System and Associated Methods | |
| US20140276361A1 (en) | Systems and methods for treating glaucoma | |
| EP2994038B1 (en) | Photoacoustic imaging and measurement of a structure at the human eye fundus | |
| US7828438B1 (en) | Method and apparatus for early diagnosis of Alzheimer's using non-invasive eye tomography by Terahertz | |
| JP2018514251A (en) | Multi-depth optical coherence tomography (OCT) system, method, and laser eye surgery system including the system | |
| WO2014145684A1 (en) | Systems and methods for treating glaucoma | |
| US9918628B2 (en) | Accommodation function evaluation apparatus | |
| Sheikh et al. | Clinical translation of a novel photoacoustic imaging system for examining the temporal artery | |
| CN108567410B (en) | Confocal synchronous imaging system for optical coherence tomography and point scanning | |
| US20170311804A1 (en) | Diffuse acoustic confocal imager | |
| KR101610837B1 (en) | Photoacoustic imaging system for diagnosis animals of the ophthalmic diseases | |
| CN115253090A (en) | Photodynamic therapy equipment at somatic cell level | |
| KR20160001890A (en) | Anti-aging skin theragnostic system using ultrasound and OCT | |
| CN113616934A (en) | Laser accurate irradiation system and method for photodynamic therapy | |
| JP2019118457A (en) | Photoacoustic apparatus, and information processing apparatus and method | |
| CN111281332A (en) | An ophthalmic multifunctional anterior segment imaging device based on a slit lamp platform | |
| CN110314034A (en) | A kind of corneal cross-linking device of achievable individual character treatment | |
| CN115399729A (en) | Visual function detection equipment and method at somatic cell level | |
| CN115517669A (en) | Ion channel detection equipment at somatic cell level | |
| US20130128277A1 (en) | Arrangement and method for interferometry |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |