CN115245174A - A kind of water-soluble compound potassium hydrogen persulfate disinfectant and preparation method thereof - Google Patents

Abstract

The invention relates to a water-soluble compound potassium hydrogen persulfate disinfectant, which comprises the following components in percentage by mass: 91-92.3% of oxidant and 7.7-9% of quaternary ammonium salt; the concentration of the aqueous solution of the compound disinfectant is 0.54-0.70% (W/V). The potassium hydrogen persulfate composite salt and the quaternary ammonium salt are compounded to have a synergistic effect, so that the ethanol-resistant microorganisms can be quickly and efficiently killed. By compounding the disinfectant, ethanol-resistant microorganisms can be effectively killed, the microbial control of the pharmaceutical environment is facilitated to be enhanced, and the pollution risk of the pharmaceutical environment is reduced.

Description

A kind of water-soluble compound potassium hydrogen persulfate disinfectant and preparation method thereof

technical field

The invention relates to a water-soluble compound potassium hydrogen persulfate disinfectant and a preparation method thereof, belonging to the technical field of disinfectants.

Background technique

Disinfectants are generally chemicals that kill potentially disease-causing microorganisms. Disinfectants can usually be divided into peroxide disinfectants, guanidine disinfectants, alcohol disinfectants, phenol disinfectants, iodine-containing disinfectants, chlorine-containing disinfectants, quaternary ammonium salt disinfectants and aldehydes according to their chemical composition. Disinfectants etc.

At present, the commonly used disinfectants in pharmaceutical companies mainly include chlorine-containing disinfectants, quaternary ammonium salt disinfectants, phenol disinfectants, alcohol disinfectants and peroxide disinfectants. Representative products of chlorine-containing disinfectants include 84 disinfectant and sporicide, which have a strong killing effect on various pathogenic microorganisms, but the presence of organic matter will affect its effect, and it is easily affected by reducing agents and the environment, and it is harmful to the respiratory tract. irritating. The representative product of quaternary ammonium salt disinfectant is Xinjieermi; its advantages are low bactericidal concentration, low toxicity and irritation, colorless solution, non-corrosiveness, low odor, good water solubility, strong surface activity and stable properties ; But the effect on non-enveloped virus is not good, and there are many incompatibility, and it is invalid to spores, which belongs to the middle-effect disinfectant. Representative products of phenolic disinfectants are acidic phenol, basic phenol, etc. They have low dilution concentration, narrow adaptability, strong odor, and high toxicity. They are not easy to decompose, and they are easy to cause environmental pollution after use. Alcohol disinfectants mainly include ethanol and isopropanol; among them, 75% ethanol has simple components, clear ingredients, non-corrosiveness, and no residual toxicity. It can be used for disinfection in clean environments and has become the most commonly used medium-efficiency disinfectant in pharmaceutical companies ; but its killing rate of spores and ethanol-resistant microorganisms is low. Peroxide disinfectant is a highly efficient and strong oxidizing disinfectant, and pharmaceutical companies mainly use hydrogen peroxide; this kind of disinfectant is effective, but it is extremely corrosive, skin irritating, and poor in stability. In pharmaceutical companies, alcohol disinfectants and peroxide disinfectants are often used alternately to ensure the disinfecting effect.

Compared with other peroxides, potassium persulfate can not only quickly and effectively kill various pathogenic microorganisms, but also has the advantages of low toxicity and environmental protection. It is harmless to humans and animals and will not pollute the environment. At present, the existing potassium persulfate compound salt disinfectant is usually composed of potassium persulfate, sodium chloride, pigments and the like. Its bactericidal mechanism is as follows: oxidation releases hypochlorous acid, which decomposes into new ecological oxygen and free chlorine, and sterilizes through oxidation. Chinese patent CN1545883A water-soluble compound potassium hydrogen persulfate powder disinfectant, CN101836659A compound potassium hydrogen persulfate disinfectant and CN102265904A compound potassium hydrogen persulfate disinfectant and manufacturing process thereof all take potassium hydrogen persulfate as the main disinfectant.

Peroxide disinfectants compounded with quaternary ammonium salt disinfectants include hydrogen peroxide, peracetic acid, ozone and sodium hypochlorite. Chinese patent CN112932995A a surface cleaning wipe and its production method, CN104206415A a kind of disinfectant water for cleaning CT machine and its preparation method, CN107396940A a kind of high-efficiency non-residue disinfectant and CN104094969A a preparation method of silkworm tool disinfectant , are all disinfectants formed by compounding quaternary ammonium salts and peroxides.

The traditional disinfectant is a single component, which is often accompanied by the disadvantages of single antibacterial effect and easy tolerance, and the killing efficiency of bacterial spores and ethanol-resistant microorganisms is not high. Pharmaceutical companies often use ethanol and peroxide disinfectants to disinfect in turn. As a medium-effect disinfectant, ethanol is the most commonly used in pharmaceutical companies. It is used alternately with other disinfectants and the frequency of use is very high, which leads to the production of ethanol-resistant bacteria in the pharmaceutical environment; Moreover, other disinfectants, such as high-efficiency peroxide disinfectants, cannot completely disinfect ethanol-resistant bacteria, so ethanol-resistant bacteria have become the main risk of microbial contamination. The invention compound the peroxide disinfectant to provide a disinfectant with good disinfecting effect on ethanol-resistant bacteria.

SUMMARY OF THE INVENTION

The object of the present invention is to overcome above-mentioned weak point of prior art and provide a kind of water-soluble compound potassium hydrogen persulfate disinfectant and preparation method thereof, the disinfecting effect of this water-soluble compound potassium hydrogen persulfate disinfectant to ethanol-resistant bacteria Stablize.

In order to achieve the above object, the technical solution adopted by the present invention is: a water-soluble compound potassium hydrogen persulfate disinfectant, said disinfectant comprising the following components in mass percentage: oxidant 71.4%～92.3%, quaternary ammonium salts 7.7% ~28.6%.

As a preferred embodiment of the disinfectant of the present invention, the disinfectant includes the following components in mass percentage: 91%-92.3% of oxidizing agent, and 7.7%-9% of quaternary ammonium salts.

As a preferred embodiment of the disinfectant of the present invention, the oxidizing agent is potassium persulfate complex salt.

As a preferred embodiment of the disinfectant of the present invention, the quaternary ammonium salt is dodecyldimethylbenzyl ammonium chloride.

Potassium persulfate compound salt can undergo a chain reaction in water to release active substances such as free radicals and nascent oxygen [O]. These substances have the characteristics of strong penetrating power and small size, and can quickly diffuse to the surface of pathogen cells, making them The inactivation of functionally related enzymes such as transport on the cell membrane causes the content to flow out, and can also enter the cell interior, denature proteins, combine with metal ions in nucleic acids, break phosphodiester bonds, and interfere with the binding of DNA and RNA.

The target of quaternary ammonium salts is the cell membrane and condensate. Its high affinity can be adsorbed to the surface of the bacteria, and the hydrophobic base can be inserted into the lipid layer, destroying the cell wall and cell membrane permeability barrier, promoting the leakage of intracellular substances, and enhancing the persulfate concentration. Potassium hydrogen has a destructive effect on cell membranes and intracellular macromolecules such as enzymes, nucleic acids and ribosomes.

Potassium hydrogen persulfate compound salts and quaternary ammonium salts have different targets and can play a good synergistic effect. Chloride ions present in the quaternary ammonium salt can be oxidized into chlorine gas by the free radicals released by the potassium persulfate compound salt, and the chlorine gas is further hydrolyzed to generate hypochlorous acid and hypochlorite, which enhances the bactericidal ability of the present invention.

As a preferred embodiment of the disinfectant of the present invention, the disinfectant is used in the form of a 0.54-0.70% (W/V) aqueous solution.

As a preferred embodiment of the disinfectant of the present invention, the disinfectant is used in the form of a 0.54% (W/V) aqueous solution.

In the second aspect, the present invention also provides a preparation method of the above-mentioned disinfectant, which is obtained by mixing the oxidizing agent and the quaternary ammonium salt according to the ratio.

Water-soluble compound potassium hydrogen persulfate disinfectant of the present invention (described disinfectant comprises the component of following mass percent: oxidizing agent 92.3%, quaternary ammonium salt 7.7%), is 0.54% (W/V) in compound disinfectant aqueous solution concentration ), that is, the final concentrations of potassium persulfate compound salt and quaternary ammonium salt are 0.503% and 0.042% (W/V) respectively, and the killing rate of ethanol-resistant bacillus can reach more than 99.999% within 3 minutes, which is fast And efficient aspect is superior to the disinfectant based on potassium monopersulfate compound salt, as CN107624785A a kind of low corrosivity potassium monopersulfate compound salt disinfectant (this disinfectant needs to kill 15 minutes under 5% concentration), CN1545883A water-soluble compound potassium hydrogen persulfate powder disinfectant (this disinfectant needs to be eliminated at 0.5% concentration for 5 minutes) and CN102265904A compound potassium hydrogen persulfate disinfectant and its manufacturing process (this disinfectant needs to be eliminated at 1% concentration) Kill 10 minutes), also be better than quaternary ammonium salt disinfectant, as CN104094969A a kind of preparation method of silkworm silkworm tool disinfectant (this disinfectant needs to kill 3 hours under 0.3% concentration of quaternary ammonium salt) and CN104206415A a kind of use Disinfectant water for cleaning CT machines and its preparation method (the disinfectant needs quaternary ammonium salt concentration of 5.0%).

Compared with the prior art, the beneficial effects of the present invention are: the water-soluble compound potassium hydrogen persulfate disinfectant of the present invention first compounded potassium hydrogen persulfate compound salt and quaternary ammonium salts, and through the synergistic effect of each component, It can quickly and effectively kill ethanol-resistant microorganisms, ensuring good microbial control in the pharmaceutical environment. It can not only reduce the concentration of disinfectant components, but also improve disinfection efficiency and broad-spectrum; the logarithm of killing ethanol-resistant bacteria within 3 minutes can be reduced Reach more than 5; under the interference of organic matter and higher temperature, the disinfecting effect on ethanol-resistant bacteria is stable; the free radical produced after the potassium persulfate compound salt of the present invention is dissolved in water has stronger oxidative disinfection effect, and can oxidize Chloride ions in the quaternary ammonium salt generate hypochlorous acid, which enhances the disinfection effect of the present invention and prolongs the disinfection time of the present invention. The invention has few components, simple preparation and convenient use.

Description of drawings

Fig. 1 is the investigation figure of dodecyl dimethyl benzyl ammonium chloride mass percentage in disinfectant.

Fig. 2 is the investigation diagram of compound disinfectant aqueous solution concentration.

Fig. 3 is the result chart of sterilization time test in embodiment 6.

Fig. 4 is the result chart of organic interfering substance test in embodiment 6.

FIG. 5 is a graph showing the results of the temperature test in Example 6.

Detailed ways

In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

Example 1

A water-soluble compound potassium hydrogen persulfate disinfectant comprises the following components in mass percentage: 71.4% of potassium hydrogen persulfate compound salt and 28.6% of dodecyl dimethyl benzyl ammonium chloride. It is used as an aqueous solution of 0.70% (W/V), and the logarithm of disinfecting ethanol-resistant bacillus within 3 minutes is 5.70.

Example 2

A water-soluble compound potassium hydrogen persulfate disinfectant comprises the following components in mass percentage: 92.3% of potassium hydrogen persulfate compound salt and 7.70% of dodecyl dimethyl benzyl chloride. It is used as an aqueous solution of 0.60% (W/V), and the logarithm of disinfecting ethanol-resistant bacillus within 3 minutes is 5.83.

Example 3

A water-soluble compound potassium hydrogen persulfate disinfectant comprises the following components in mass percentage: 76.9% of potassium hydrogen persulfate compound salt and 23.1% of dodecyl dimethyl benzyl chloride. It is used as an aqueous solution of 0.65% (W/V), and the logarithm of disinfecting ethanol-resistant bacillus within 3 minutes is 5.47.

Example 4

A water-soluble compound potassium hydrogen persulfate disinfectant comprises the following components in mass percentage: 91.24% of potassium hydrogen persulfate compound salt and 8.76% of dodecyl dimethyl benzyl chloride. It is used as an aqueous solution of 0.55% (W/V), and the logarithm of disinfecting ethanol-resistant bacillus within 3 minutes is 5.61.

Example 5

A water-soluble compound potassium hydrogen persulfate disinfectant comprises the following components in mass percentage: 92.3% of potassium hydrogen persulfate compound salt and 7.7% of dodecyl dimethyl benzyl chloride. It is used as an aqueous solution of 0.54% (W/V), and the logarithm of disinfecting ethanol-resistant bacillus within 3 minutes is 5.61.

The disinfecting effect on ethanol-resistant bacteria is 3.7 times that of single-component potassium hydrogen persulfate compound salt, the disinfecting effect on ethanol-resistant bacteria is 3.4 times that of single-component quaternary ammonium salt, and the disinfecting effect on ethanol-resistant bacteria is 6.4 times that of 75% ethanol. The disinfecting effect of the invention on ethanol-resistant bacteria is better than that of compounding of quaternary ammonium salt and other peroxides.

Example 6

(1) Preparation of bacterial suspension

Wet a sterile cotton swab with TPS liquid medium, scrape the bacterial lawn and transfer it to an Erlenmeyer flask containing sterilized TPS medium, shake it for 20s to make it evenly dispersed, measure its OD600 value, and calculate it according to formula 1 to make it contain bacteria The amount is 10 ⁸ cfu/mL-5×10 ⁸ cfu/mL.

y=60.965x-10.333, r ² =0.9908 Formula 1

(2) Optimization of disinfectant formula

The mixed disinfectant powder of lauryl dimethyl benzyl ammonium chloride and potassium hydrogen persulfate compound salt of different mass percentages is prepared, and then configured into disinfectant aqueous solution.

Add 0.5mL of bacterial suspension and 2mL of disinfectant aqueous solution into 5mL EP tube respectively, mix well, and act for 3 minutes, absorb 0.25mL test solution and add it to 2.25mL D/E broth neutralizer, mix well, and act for 10 minutes .

Gradually dilute to an appropriate concentration, count viable bacteria, and calculate the kill logarithm and kill rate.

The mass percentages of oxidant dodecyldimethylbenzyl ammonium chloride in the compound disinfectant powder are respectively configured as 0%, 1.96%, 3.85%, 5.66%, 7.41%, 7.70%, 9.09%, 16.7%, 23.08%, 28.57% compound disinfectant powder, then configured into 0.50% (W/V) aqueous solution, measure the kill logarithm and killing rate of each concentration according to the above-mentioned test operation, investigate the most effective effect of dodecyl dimethyl benzyl ammonium chloride Appropriate mass percentage.

According to the optimum mass percentage of lauryl dimethyl benzyl ammonium chloride, the compound disinfectant powder is configured, and then it is configured into aqueous solutions of different concentrations, and the optimal compound disinfectant aqueous solution is screened with the evaluation index of killing logarithm and killing rate. Appropriate concentration.

Test result as shown in Figure 1, when dodecyl dimethyl benzyl ammonium chloride mass percentage is 7.7% and above in compound disinfectant aqueous solution, compound disinfectant aqueous solution kills logarithm all greater than 5, because dodecyl The toxicity of dimethyl benzyl ammonium chloride increases with concentration, so the optimum mass percentage of dodecyl dimethyl benzyl ammonium chloride is 7.7% (correspondingly, persulfate The optimum mass percentage of hydrogen potassium compound salt is 92.3%), and this disinfectant powder is made into the aqueous solution of 0.60% (W/V) to use. As shown in Figure 2, when compound disinfectant aqueous solution concentration reaches 0.54% (W/V), disinfecting index reaches more than 5.0, so this compound disinfectant powder is made into the aqueous solution of 0.54% (W/V) and uses.

(3) Bactericidal effect test

Prepare bacterium suspension, prepare compound disinfectant aqueous solution by the method in embodiment 5, use the aqueous solution of 0.54% (W/V) of one-component potassium hydrogen persulfate compound salt, one-component dodecyl dimethyl benzyl The 0.54% (W/V) aqueous solution of ammonium chloride was used as a control, and 75% ethanol, a commonly used disinfectant in the pharmaceutical environment, was added as a control to conduct a comparative study on the bactericidal effect.

Add 0.5mL of bacterial suspension and 2mL of initial concentration disinfectant to four 5mL EP tubes, so that the disinfectant in the mixed solution reaches the final concentration, and act for 3 minutes respectively.

Take 0.25mL of the sample solution of each action time and add it to 2.25mL D/E broth neutralizer, mix well, and act for 10min.

Draw 0.5mL sample solution, dilute to an appropriate concentration, and count viable bacteria.

At the same time, the diluted solution was used instead of the disinfectant as the control group, and a parallel test was carried out. The test results are shown in Table 1.

Table 1

group disinfect logarithm kill rate One-component potassium hydrogen persulfate compound salt 1.51 96.909% One-component dodecyl dimethyl benzyl ammonium chloride 1.65 97.761% 75% ethanol 0.87 86.510% Compound potassium hydrogen persulfate compound salt 5.61 99.999%

As can be seen from Table 1, the single-component potassium hydrogen persulfate compound salt, the single-component dodecyl dimethyl benzyl ammonium chloride and 75% ethanol are far below 5.0 for the disinfect logarithm of ethanol-resistant bacteria . Compared with the single component of the same concentration, the compound potassium hydrogen persulfate disinfectant of the present invention has the highest killing logarithm, and the killing rate is as high as 99.999%, and can efficiently kill ethanol-resistant bacteria.

(4) Sterilization time test

Prepare bacterial suspension, prepare disinfectant by the method for embodiment 5.

Add 0.5mL bacterial suspension and 2mL initial concentration of disinfectant to four 5mL EP tubes to make the disinfectant in the mixed solution reach the final concentration, and act for 1min, 2min, and 3min respectively.

Take 0.25mL of the sample solution of each action time and add it to 2.25mL D/E broth neutralizer, mix well, and act for 10min.

Draw 0.5mL sample solution, dilute to an appropriate concentration, and count viable bacteria.

At the same time, the diluted solution was used instead of the disinfectant as a control group for parallel experiments.

As shown in Figure 3, the kill logarithm of 2 minutes and 3 minutes of sterilization time reaches more than 5.0, so the sterilization time is selected as 2-3 minutes. The action time of this compound disinfectant is lower than the disinfectant specification requirement of 5 minutes, which can quickly disinfect ethanol-resistant bacteria .

(5) Test of organic interfering substances

Prepare disinfectant and bacterial suspension according to the above method, put sterile milk, bacterial suspension and disinfectant in a constant temperature water bath at (20±2)°C, and let it stand for 5 minutes.

Test group: Add 0.25mL of bacterial suspension and 0.25mL of sterile milk into a 5mL EP tube, mix well, and make a bacterial suspension containing 50% organic interfering substances. Then add 2mL of the disinfectant of Example 5, mix evenly, and according to the bactericidal test result, the action time is 3 minutes. Draw 0.25mL of the above liquid into a 5mL EP tube containing 2.25mL of neutralizing agent, and act for 10 minutes.

Positive control group: Add 0.5mL of bacterial suspension and 2mL of the disinfectant of Example 5 to a 5mL EP tube, mix well, and after 3 minutes of action, absorb 0.25mL of the above liquid and add it to a 5mL EP tube containing 2.25mL of neutralizing agent. 10 minutes.

Negative control group: add 0.5mL bacterial suspension and 2mL TPS diluent to a 5mL EP tube, mix well, and act for 3 minutes, then draw 0.25mL of the above liquid into a 5mL EP tube containing 2.25mL neutralizer, and act for 10 minutes.

The above-mentioned three groups of sample liquids were taken for gradient dilution and coating, cultured at 37°C for 24 hours, the number of colonies was counted, and the killing rate and logarithmic value of killing were calculated.

In the test group, the 50% organic interfering substance bacterial suspension was replaced with 25% organic interfering substance bacterial suspension, and then the above test was carried out.

As shown in Figure 4, the disinfect logarithm of the compound disinfectant was greater than 5.0 when 50% and 25% milk acted. According to the significance analysis, P was greater than 0.01, so the organic matter interference was not significant.

(6) Temperature test

Prepare bacterial suspension and disinfectant as above.

Take two 5mL EP tubes, add 2mL of the disinfectant of Example 5 and 2mL of TPS solution respectively as test group and control group, and then add 2.25mL neutralizer into the above-mentioned EP tubes respectively.

Place the EP tube in a constant temperature water bath at (40±1)°C and let it stand for 3 minutes.

Carry out the bactericidal test according to the suspension quantitative method, dilute to a suitable gradient, carry out the counting of live bacteria culture, count the test results, and calculate the average killing logarithm value and killing rate.

Carry out a parallel test at each temperature, and repeat the above test at (30±1)°C.

As shown in Figure 5, at higher temperatures of 30°C and 40°C, the disinfect logarithm of the compound disinfectant can still maintain more than 5.0, and there is no significant change (P>0.01), so the compound disinfectant at higher temperatures The bactericidal effect is more stable.

(7) Investigation on the combined effect of compound components

In order to investigate whether the combination of two components in the compound disinfectant has a synergistic effect, according to the preferred disinfectant powder formula ratio, prepare 0.54% (W/V) compound disinfectant aqueous solution and a single-component aqueous solution of the same concentration ( 0.042% (W/V) dodecyl dimethyl benzyl ammonium chloride aqueous solution and 0.503% (W/V) potassium hydrogen persulfate complex salt solution), and adopt the two-fold dilution method to measure the single The minimum inhibitory concentration (MIC) of the component aqueous solution and the fractional inhibitory concentration (FIC) when used in combination.

Take 10mL of the above-mentioned antibacterial solution of different concentrations and add it to a sterile petri dish, then take 10mL of TSA medium and add it to the above-mentioned petri dish, shake the plate gently while adding, so that the antibacterial solution and the medium are evenly mixed, and wait for it to completely solidify before use .

Take 200 μL of the bacterial suspension and add it to the solidified medium, spread the bacterial suspension evenly with a triangular spreader, and place it in a 37°C incubator for 24 hours. After the culture is completed, the growth of the bacteria is observed, and the minimum corresponding concentration in the culture medium that is completely sterile is the minimum inhibitory concentration (MIC) of the antibacterial drug.

Based on the MICs of the two kinds of antibacterial substances measured in the above test, two kinds of antibacterial solutions of 4MIC were first prepared, and then antibacterial solutions of 2MIC, 1MIC, 1/2MIC, and 1/4MIC were prepared respectively by the double dilution method, and the concentrations of each The two antibacterial solutions are used in combination, that is, take 5mL of the two antibacterial solutions and 10mL TSA medium respectively and add them to a sterile petri dish, shake well, inoculate X2 bacteria after solidification, and cultivate them in a 37°C incubator for 24 hours, and observe the Growth status, the minimum corresponding concentration of sterile growth is the MIC size of the combined effect.

See formula 2 for the calculation formula of graded inhibitory concentration.

The criteria for judging FIC are as follows: when FIC≤0.5, the two antibacterial drugs used in combination exert synergistic effect; when 0.5<FIC≤1.0, there is an additive effect; antagonism. The test results are shown in Table 2.

Table 2

Note: "-" stands for sterile growth, "+" stands for bacterial growth

The minimum inhibitory concentrations of dodecyl dimethyl benzyl ammonium chloride and potassium hydrogen persulfate compound salt were measured to be 0.0525g/L and 2.52g/L respectively. The combination effect test results are shown in Table 2, wherein MIC _A and MIC _B are respectively the MIC values of potassium hydrogen persulfate compound salt and dodecyl dimethyl benzyl ammonium chloride, obtained from Table 2, for ethanol-resistant microorganisms In other words, the MIC value of the joint action of potassium persulfate compound salt and dodecyl dimethyl benzyl ammonium chloride is 1/8MIC _A + 1/4MIC _B , which are 1/8 and 1/8 of the MIC value when used alone. /4, thus the FIC value after the two can be calculated is 0.375, and according to the judgment standard, after potassium persulfate compound salt and dodecyl dimethyl benzyl ammonium chloride are used in combination, the resistance to Ethanol microbes exhibit synergy.

Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that The technical solution of the present invention can be modified or equivalently replaced without departing from the spirit and scope of the technical solution of the present invention.

Claims (7)

1. The water-soluble compound potassium hydrogen persulfate disinfectant is characterized by comprising the following components in percentage by mass: 71.4 to 92.3 percent of oxidant and 7.7 to 28.6 percent of quaternary ammonium salt.

2. The disinfectant as set forth in claim 1, wherein the disinfectant comprises the following components in percentage by mass: 91 to 92.3 percent of oxidant and 7.7 to 9 percent of quaternary ammonium salt.

3. The disinfectant of claim 1 or 2 wherein said oxidizing agent is oxone complex salt.

4. The disinfectant of claim 1 or 2, wherein said quaternary ammonium salt is dodecyl dimethyl benzyl ammonium chloride.

5. Disinfectant according to claim 1 or 2, characterized in that it is formulated for use as an aqueous solution of 0.54-0.70% (W/V).

6. The disinfectant of claim 5, wherein said disinfectant is formulated for use as a 0.54% (W/V) aqueous solution.

7. The disinfectant according to claims 1 to 6, wherein said disinfectant is prepared by mixing an oxidant and a quaternary ammonium salt in a ratio.

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