CN115227804A - A kind of hair growth liquid for preventing seborrheic alopecia and promoting hair growth and preparation method thereof - Google Patents

Abstract

The invention discloses a hair growth liquid for preventing seborrheic alopecia and promoting hair growth and a preparation method thereof, belonging to the technical field of hair growth liquid and comprising the following raw materials in percentage by weight: 1-6% of helichrysum extract, 1-6% of biota orientalis extract, 0.005-0.03% of acetyl tetrapeptide-3,0.01-0.1% of myristoyl pentapeptide-17,0.005-0.025% of biotin 3 peptide-1,0.01-0.02% of fullerene derivative and the like; can promote the proliferation and differentiation of hair follicle cells, reduce the damage of dihydrotestosterone to the health of hair follicles, and provide sufficient nutrients for promoting keratin expression and synthesis. The addition of acetyl tetrapeptide-3 and biotin tripeptide-1 promotes the proliferation and differentiation of hair follicle cells; myristoyl pentapeptide-17 provides nutrients sufficient to promote keratin expression and synthesis, and promotes hair formation and growth.

Description

A kind of hair growth liquid for preventing seborrheic alopecia and promoting hair growth and preparation method thereof

technical field

The invention belongs to the technical field of daily chemical industry and washing and care, in particular to a hair growth liquid for preventing seborrheic alopecia and promoting hair growth and a preparation method thereof.

Background technique

Seborrheic alopecia (SA), also known as androgenetic alopecia, is the most common clinical hair loss disorder. According to the survey, more than 80% of hair loss patients are seborrheic alopecia or accompanying seborrheic alopecia. Epidemiological surveys show that it is increasing year by year, and it shows a younger trend, which not only hinders the appearance and affects the appearance, but also brings huge mental pressure and psychological burden to patients, which seriously affects the quality of life of patients.

The main clinical manifestations of male seborrheic alopecia are that the hairline of the forehead, especially the two temples, gradually moves up and the hair on the top of the head is thinning, and the hair on the forehead and the top of the head gradually falls off. Seborrheic alopecia is mainly characterized by diffuse alopecia, with progressive reduction and thinning of hair in the central area of the scalp, but no obvious changes in the forehead hairline. The disease is significantly associated with genetic factors and abnormal scalp androgen metabolism. Patients with a family history of the disease progress faster and tend to have early onset with age.

Seborrheic alopecia is mainly caused by abnormal androgen and its receptors, and it is also related to genetic and psychological factors; modern medicine believes that SA is an androgen-dependent autosomal dominant polygenic hereditary alopecia with excess androgen. Or increased sensitivity to androgen is the main factor in the occurrence of the disease. The occurrence of SA is directly related to the increased levels of testosterone (T) and its metabolites dihydrotestosterone (DHT) and androgen receptor (AR), while 5α-reductase (5α-R) plays an important role in it. In addition, long-term psychological imbalance, mental stress, eating disorders and bacterial infection are important factors that induce or aggravate the disease.

The inventors believe that to solve seborrheic alopecia systematically, three aspects need to be coordinated at the same time: one is to promote the proliferation and differentiation of hair follicle cells; the other is to reduce the damage of dihydrotestosterone (DHT) to the health of the hair follicle; Synthetic nutrients. The currently approved drugs are only aimed at a certain aspect of the influencing factors, and some hair growth nutrient solution products have the problem of unclear composition targets and weak pertinence.

At present, the main medical methods for the treatment of seborrheic alopecia are topical minoxidil and oral 5α-reductase (5α-R) inhibitor androsteroids such as finasteride and dutasteride, and finasteride. Amines are predominant.

Minoxidil is a non-specific drug for the treatment of hair loss, commonly used in concentrations of 20 and 50 mg/mL, and is the only topical drug approved by the FDA for the treatment of seborrheic alopecia. Its mechanisms for preventing and treating hair loss include: opening potassium ion channels, inhibiting calcium ion inflow, reducing the inhibition of epidermal growth factor on hair growth, promoting the proliferation and differentiation of hair follicle epithelial cells, up-regulating vascular endothelial growth factor and improving local blood supply, etc. However, it may cause facial and limb hirsutism during use, and the therapeutic effect gradually disappears after discontinuation. Some users experience scalp itching and dandruff adverse reactions, and there are also many users who grow small hair, and the hair cannot continue to thicken and grow.

At present, there are many synthetic steroidal and non-steroidal 5α-R inhibitors on the market. However, these synthetic inhibitors often have many adverse reactions, and it is difficult to meet the needs of the majority of patients. Finasteride is a steroid-selective inhibitor specific for type II 5α-reductase (5α-R), which can reduce the conversion of testosterone (T) into dihydrotestosterone (DHT), which binds more strongly to the receptor , thereby attenuating the effect of androgens on hair loss. Finasteride can continue to improve hair growth, but it has adverse effects such as abnormal sexual function, sperm reduction and gynecomastia.

In addition to drug treatment, there are also some complex plant ingredients and polypeptide complex ingredients hair growth liquid on the market. The function of the plant components of these hair growth liquids is described as promoting blood circulation rather than targeting specific substances. There are unclear targets for the action of the components, the synergistic mechanism of the plant components is unclear, the effect is slow, and the target components are targeted in the production and extraction technology. Not strong, quantitatively inaccurate defects.

5α-R inhibitors in plants have broad development prospects. In the past two years, a variety of plant extracts have been reported to have strong 5α-R inhibitory activities. Therefore, screening out 5α-R inhibitors with clear effect and significant activity from plants, which will be used in a new generation of seborrheic alopecia health products with a clear pharmacodynamic material basis, will bring good news to patients with seborrheic alopecia at home and abroad.

SUMMARY OF THE INVENTION

In order to solve the above technical problems, the present invention provides a hair growth liquid for preventing seborrheic alopecia and promoting hair growth and a preparation method thereof.

The object of the present invention can be realized through the following technical solutions:

A hair growth liquid for preventing seborrheic alopecia and promoting hair growth, comprising the following raw materials by weight: 0.005-0.03% of acetyl tetrapeptide-3, 0.01-0.1% of myristoyl pentapeptide-17, 0.005-0.025% of biological 3-Peptide-1, 1-6% Helichrysum extract, 1-6% Platycladus orientalis leaf extract, 0.2-2% estrogen Chinese medicine extract, 0.2-2% Panthenol, 0.2-0.5 % phenoxyethanol, 0.5-3% cornflower extract, 0.01-0.02% fullerene derivative, and the balance is alcohol-water buffer.

Acetyl Tetrapeptide-3:

The size of the hair follicle is determined by the size of the dermal papilla and extracellular matrix. Healthy dermal papillae produce extracellular matrix proteins such as collagen III and anchoring fibers such as laminin collagen VII, which strengthen the hair roots. If there is a problem with extracellular matrix turnover, hair can become brittle. If this cycle continues, the hair follicles will eventually shrink. Acetyl tetrapeptide-3 accelerates the synthesis of extracellular matrix proteins such as laminin, collagen III and collagen VII through the synthesis of fibroblasts, and directly acts on the tissue around the hair follicle to increase the volume and length of the hair follicle. Repairs the connective tissue between the epidermis and the dermis, and promotes the fixation of the hair in the follicle.

Myristoyl Pentapeptide-17:

The control of the hair growth cycle is related to the complex interaction between the two tissues, the hair bulge and the dermal papilla, which promote hair growth due to the nerves in the dermal papilla and the blood supply that nourishes the hair. Myristoyl Pentapeptide-17 is an effective hair growth-promoting pentapeptide that acts directly on keratin genes to promote keratin production.

Biotin 3 Peptide-1:

It is a tripeptide that combines vitamin H and Matrikine series signal peptide GHK. Biotin 3-peptide-1 increases the synthesis of extracellular matrix such as collagen IV and laminin 5, delays the aging of hair follicles, improves the structure of hair follicles, and helps to fix the hair in the dermal follicle to prevent hair loss. Activation of tissue repair gene expression is beneficial to the reconstruction and repair of skin structure. Promotes cell proliferation and differentiation and stimulates hair growth.

Helichrysum Extract:

Also known as permanent flower, it is native to Europe, West Asia and North America, and is also cultivated in Xinjiang and Shandong in my country. It is a medicinal herb of the Compositae family. The active ingredient in its extract is helichrysum flavonoids, and the total flavonoid content is very high. The substance has a strong inhibitory effect on the activity of 5α-R reductase in the 5α-R reductase in vitro test. In addition, the reduction reaction of 5α-R is inhibited, so that testosterone (T) cannot be reduced to dihydrotestosterone (DHT), and the inhibition and toxicity of androgens to hair follicles are reduced.

Arborvitae Leaf Extract:

It is an evergreen tree of Cypressaceae, which is a special medicinal plant in China. The active ingredients in its extract are thuja flavonoids and quercetin. The substance has a strong inhibitory effect on the activity of 5α-R reductase in the 5α-R reductase in vitro test. In addition, the reduction reaction of 5α-R is inhibited, so that testosterone (T) cannot be reduced to dihydrotestosterone (DHT), and the inhibition and toxicity of androgens to hair follicles are reduced.

Sophora Radix Extract:

The active ingredient in its extract is Sophora flavanone. Its role is to improve capillary microcirculation, has estrogen-like effects (androgen antagonism), and also has certain 5α-R reductase inhibitory properties.

Astragalus Extract:

The active ingredients in the extract are astragalus flavonoids, verbasco isoflavones and astragaloside. Improve capillary microcirculation, have estrogen-like effects (androgen antagonism), and also have certain 5α-R reductase inhibition.

Ligustrum lucidum extract:

The active ingredients in its extract are lucidum flavonoids and oleanolic acid. Improve capillary microcirculation, have estrogen-like effects (androgen antagonism), and also have certain 5α-R reductase inhibition.

Panthenol:

Also known as provitamin B5, penetrating moisturizer, moisturizing the stratum corneum, opening skin channels and helping absorb other active ingredients. Stimulates epidermal cell growth, inhibits inflammation, and protects mucous membranes. Provides nourishment for hair growth.

Further, described fullerene derivative comprises the following steps to prepare:

Step S1, adding carbon 60 to the three-necked flask, slowly adding liquid bromine, magnetic stirring at room temperature for 72-96h, vacuum filtration after the reaction, the extracted bromine vapor is absorbed with anhydrous sodium sulfite solution, and the filter cake is After standing at room temperature for 24h, vacuum drying at 60°C for 10h to obtain modified fullerene, and the dosage ratio of carbon 60 and liquid bromine is controlled to be 400-500mg:100mL;

Step S2, adding the modified fullerene into anhydrous ethanol, stirring magnetically for 30 minutes, adding 13% aqueous sodium hydroxide solution dropwise, stirring at a constant speed at room temperature and reacting for 6 hours, after the reaction, add concentrated hydrochloric acid dropwise to adjust pH, until pH= 5-5.6, centrifuge, collect the supernatant, spin the supernatant at 45°C, extract the spin-evaporated product with acetone, repeat three times to obtain a crude product, purify, obtain a fullerene derivative, and control the modification to enrich Lenene, absolute ethanol and aqueous sodium hydroxide solution 200-250mg: 25-30mL: 40mL.

In step S1, carbon 60 is modified by liquid bromine to prepare modified fullerene, which is bromofullerene, and then in step S2, bromofullerene, ethanol and sodium hydroxide are used as raw materials to prepare fullerene Derivatives, which are amphiphilic fullerene derivatives with hydroxyl and ethoxy groups, can be used as a drug on the one hand, and the amphiphilicity enables it to smoothly pass through the hydrophilic and lipophilic regions of the cell membrane and interact with the organism. On the other hand, it increases the solubility of trace essential oils in oil-soluble drugs such as oriental orientalis leaf extract in vivo, and effectively improves the effect of drugs.

Further, the helichrysum extract, the oriental orientalis leaf extract, the estrogenic Chinese medicine extract and the cornflower extract are prepared by the following steps:

Helichrysum, oriental orientalis leaves, estrogen-based Chinese herbal medicines and cornflower were placed in an oven, dried at 40-45 ° C for 2 hours, and then the volume fraction of 50% ethanol aqueous solution was used as the extraction solvent, according to the ratio of material to liquid 1: : The ratio of 10 was added into a sealed container, soaked at 60 ° C for 2 hours, then kept warm and ultrasonicated at 40 Hz for 2 hours, and filtered to obtain Helichrysum extract, Platycladus orientalis leaf extract, estrogenic Chinese medicine extract and cornflower respectively. Extract.

Further, the estrogenic Chinese herbal medicine is prepared by mixing any one or more of Astragalus, Sophora flavescens and Ligustrum lucidum in any proportion.

Further, the alcohol-water buffer is prepared by mixing 1,3-propanediol and deionized water in a weight ratio of 1:50-60.

A preparation method of a hair growth liquid for preventing seborrheic alopecia and promoting hair growth, comprising the steps of:

Acetyl tetrapeptide-3, myristoyl pentapeptide-17, biotin 3-peptide-1, Helichrysum extract, Platycladus orientalis leaf extract, estrogenic Chinese medicine extract, panthenol, phenoxyethanol, arrowroot The chrysanthemum extract and the fullerene derivative are mixed and shaken by vortex for 15-30 minutes, and then an alcohol-water buffer is added to prepare a hair growth solution for preventing seborrheic alopecia and promoting hair growth.

Beneficial effects of the present invention:

The hair growth liquid for preventing seborrheic alopecia and promoting hair growth prepared by the invention can promote the proliferation and differentiation of hair follicle cells, reduce the damage of dihydrotestosterone (DHT) to the health of hair follicles, and provide sufficient nutrients for promoting keratin expression and synthesis. Additives acetyl tetrapeptide-3 and biotin tripeptide-1 promote the proliferation and differentiation of hair follicle cells; Helichrysum extract and Arborvitae leaf extract inhibit 5α-R reduction reaction and reduce the damage of dihydrotestosterone (DHT) to hair follicle health , to eliminate the inhibition of hair follicle growth; myristoyl pentapeptide-17 provides sufficient nutrients to promote keratin expression and synthesis, and promote hair formation and growth.

Helichrysum extract and Arborvitae leaf extract can effectively inhibit the 5α-R reduction reaction, reduce the damage of dihydrotestosterone (DHT) to the health of hair follicles, and eliminate the inhibition of hair follicle growth. There are no adverse effects of androgens on the body.

Polypeptides are added in the formula of the present invention, which has the following effects:

a. Strong activity: high activity, low dosage can be effective.

b. The effect is gentle and precise: Different from hormones and injection products, the important feature of peptides is that the effect is moderate but precise.

c. High safety: Due to the homology of peptides and body substances, it is guaranteed that it has high compatibility on the skin and avoids the potential safety hazards caused by injection.

d. High absorbability: Compared with collagen, the molecular weight of polypeptide is smaller, and it can penetrate into the skin to play a role.

e. Quality controllability: Each active polypeptide used is a single substance. Compared with plant extracts, its quality is easier to control, and the purity can reach more than 98%.

Moreover, the solvent of the present invention is an alcohol-water buffer, and the solvent has good compatibility with each component, is not greasy, and can also be used in combination with minoxidil liniment. The hair growth liquid prepared by the present invention is used when Apply to the hair root and scalp, 2-5mL each time, 1-2 times a day, massage to absorb.

Detailed ways

The technical solutions in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

Example 1

Described fullerene derivative comprises the following steps to prepare:

Step S1, add carbon 60 into the three-necked flask, slowly drop liquid bromine, stir magnetically for 72h at room temperature, filter under reduced pressure after the reaction, absorb the extracted bromine vapor with anhydrous sodium sulfite solution, and filter the cake at room temperature After standing for 24h, vacuum drying at 60°C for 10h to prepare modified fullerene, and the dosage ratio of carbon 60 and liquid bromine is controlled to be 400mg:100mL;

Step S2, adding the modified fullerene into anhydrous ethanol, stirring magnetically for 30 minutes, adding 13% aqueous sodium hydroxide solution dropwise, stirring at a constant speed at room temperature and reacting for 6 hours, after the reaction, add concentrated hydrochloric acid dropwise to adjust pH, until pH= 5. Centrifuge, collect the supernatant, spin the supernatant at 45°C, extract the spin-evaporated product with acetone, repeat three times to obtain the crude product, purify, obtain the fullerene derivative, and control the modification of the fullerene , anhydrous ethanol and sodium hydroxide aqueous solution 200mg: 25mL: 40mL.

Example 2

Described fullerene derivative comprises the following steps to prepare:

Step S1, add carbon 60 into the three-necked flask, slowly add liquid bromine, magnetic stirring at room temperature for 80h, after the reaction is completed, filter under reduced pressure, absorb the extracted bromine vapor with anhydrous sodium sulfite solution, and filter the cake at room temperature After standing for 24h, vacuum-drying at 60°C for 10h to obtain modified fullerene, and the dosage ratio of carbon 60 and liquid bromine was controlled to be 450mg:100mL;

Step S2, adding the modified fullerene into anhydrous ethanol, stirring magnetically for 30 minutes, adding 13% aqueous sodium hydroxide solution dropwise, stirring at a constant speed at room temperature and reacting for 6 hours, after the reaction, add concentrated hydrochloric acid dropwise to adjust pH, until pH= 5.2, centrifuge, collect the supernatant, spin the supernatant at 45°C, extract the product of the spin-evaporation with acetone, repeat three times to obtain the crude product, purify, obtain the fullerene derivative, and control the modification of the fullerene , anhydrous ethanol and sodium hydroxide aqueous solution 220mg: 28mL: 40mL.

Example 3

Described fullerene derivative comprises the following steps to prepare:

Step S1, add carbon 60 into the three-necked flask, slowly add liquid bromine dropwise, magnetically stir at room temperature for 96h, filter under reduced pressure after the reaction, absorb the extracted bromine vapor with anhydrous sodium sulfite solution, and filter the cake at room temperature After standing for 24h, vacuum-drying at 60°C for 10h to prepare modified fullerene, and the dosage ratio of carbon 60 and liquid bromine was controlled to be 500mg:100mL;

Step S2, adding the modified fullerene into anhydrous ethanol, stirring magnetically for 30 minutes, adding 13% aqueous sodium hydroxide solution dropwise, stirring at a constant speed at room temperature and reacting for 6 hours, after the reaction, add concentrated hydrochloric acid dropwise to adjust pH, until pH= 5.6, centrifuge, collect the supernatant, spin the supernatant at 45°C, extract the spin-evaporated product with acetone, repeat three times to obtain the crude product, purify, obtain the fullerene derivative, and control the modification of the fullerene , anhydrous ethanol and sodium hydroxide aqueous solution 250mg:30mL:40mL.

Example 4

A hair growth liquid for preventing seborrheic alopecia and promoting hair growth, comprising the following raw materials by weight percentage: 0.005% of acetyl tetrapeptide-3, 0.01% of myristoyl pentapeptide-17, 0.005% of biotin 3-peptide-1, 1% Helichrysum extract, 1% Arborvitae leaf extract, 0.2% Astragalus extract, 0.2% panthenol, 0.2% phenoxyethanol, 0.5% cornflower extract, 0.01% rich lerene derivatives, and the balance is alcohol-water buffer.

The hair growth liquid is made by the following steps:

Acetyl Tetrapeptide-3, Myristoyl Pentapeptide-17, Biotin 3 Peptide-1, Helichrysum Extract, Arborvitae Leaf Extract, Astragalus Extract, Panthenol, Phenoxyethanol, Cornflower Extract After mixing with the fullerene derivative, the solution was vortexed for 15 minutes, and then an alcohol-water buffer was added to prepare a hair growth solution for preventing seborrheic alopecia and promoting hair growth.

Example 5

A hair growth liquid for preventing seborrheic alopecia and promoting hair growth, comprising the following raw materials by weight percentage: 0.01% of acetyl tetrapeptide-3, 0.05% of myristoyl pentapeptide-17, 0.015% of biotin 3-peptide-1, 3% Helichrysum extract, 3% Arborvitae leaf extract, 1% Ligustrum lucidum extract, 1% Panthenol, 0.3% Phenoxyethanol, 1.5% Cornflower extract, 0.01% fullerene derivatives, and the balance is alcohol-water buffer.

The hair growth liquid is made by the following steps:

Acetyl tetrapeptide-3, myristoyl pentapeptide-17, biotin 3-peptide-1, Helichrysum extract, Arborvitae leaf extract, Ligustrum lucidum extract, Panthenol, Phenoxyethanol, Cornflower The extract and the fullerene derivative were mixed and vortexed for 20 minutes, and then an alcohol-water buffer was added to prepare a hair growth solution for preventing seborrheic alopecia and promoting hair growth.

Example 6

A hair growth liquid for preventing seborrheic alopecia and promoting hair growth, comprising the following raw materials by weight percentage: 0.03% acetyl tetrapeptide-3, 0.1% myristoyl pentapeptide-17, 0.025% biotin 3 peptide-1, 6% Helichrysum extract, 6% Arborvitae leaf extract, 2% Sophora flavescens extract, 2% Panthenol, 0.5% Phenoxyethanol, 3% Cornflower extract, 0.02% Fullerene derivatives, the balance is alcohol-water buffer.

The hair growth liquid is made by the following steps:

Extract Acetyl Tetrapeptide-3, Myristoyl Pentapeptide-17, Biotin 3 Peptide-1, Helichrysum Extract, Arborvitae Extract, Sophora Radix Extract, Panthenol, Phenoxyethanol, Cornflower Extract After mixing with the fullerene derivative and vortexing for 30 min, the alcohol-water buffer was added to prepare a hair growth solution for preventing seborrheic alopecia and promoting hair growth.

Comparative Example 1

Compared with Example 4, no fullerene derivative was added in this comparative example.

Comparative Example 2

Compared with Example 4, the formula of this comparative example is adjusted to: 1% Helichrysum extract, 1% Platycladus orientalis leaf extract, 0.2% Astragalus extract, 0.2% Panthenol, 0.2% Phenoxy Ethanol, 0.5% cornflower extract, 0.01% fullerene derivatives, and the balance is alcohol-water buffer.

Comparative Example 3

This comparative example is a commercially available topical minoxidil hair growth solution.

Comparative Example 4

This comparative example is finasteride tablets, 5mg x 10 tablets/box, which is prepared into a 2% solution with a volume fraction of 75% ethanol before the experiment.

Comparative Example 5

Compared with Example 4, this comparative example does not add immortelle extract in the formula.

Comparative Example 6

Compared with Example 4, this comparative example does not add oriental orientalis leaf extract in the formula.

Comparative Example 7

Compared with Example 4, this comparative example does not add astragalus extract in the formula.

The hair growth solutions prepared in Examples 4-6 and Comparative Examples 1-3 were used to evaluate the efficacy of hair growth through animal experiments on C57BL/6 mice, and the results are shown in Table 1 below;

Experimental process: 110 C57BL/6 mice, half male and half, 6 weeks old, numbered according to the weight of the mice, and then randomly divided into Example 1 group, Example 2 group, Example 3 group, implementation Example 4 group, Example 5 group, Example 6 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, Minoxidil group, 10 in each group. After being anesthetized with ether, it was melted with a rosin/paraffin mixture (1:1) and then applied to the back of the mouse. After it was solidified and hardened, it was removed. From the second day after depilation, apply the corresponding hair growth liquid to the depilation area, twice a day, 2mL each time, for 30 days. During the period, the newly grown hairs on the back of the mice and the parts where the samples were not smeared were observed. Every time the image analyzer was used for the newly grown hairs at the smeared sample sites and the newly grown hairs at the non-smeared sample sites. The ratios of the two before and after were compared, and the situation of each mouse was calculated separately and the average value was calculated.

Table 1

It can be seen from Table 1 above that the hair growth liquid prepared in Examples 4-6 has a remarkable curative effect, and various active ingredients in the hair growth liquid have synergistic ability in promoting hair growth.

Clinical Trials

According to the results of animal experiments, a potential hair growth solution was selected for clinical trials, and the hair growth solutions prepared in Examples 4-6 and Comparative Examples 1-3 were clinically tested to clinically test the effect of the hair growth solution of the present invention on promoting hair growth. The subjects were 125 people aged 20-55 with alopecia areata, alopecia totalis or baldness, including 63 males and 62 females. The test personnel were randomly divided into 5 groups, 25 people in each group, respectively using Examples 4-6 and minoxidil hair growth liquid, the use method is to take about 2-5 mL of hair growth liquid, 1-2 times a day, apply to hair loss Partially, once a day in the morning and evening, massage and absorb, and use it continuously for two months. Efficacy evaluation criteria are: 1) Marked effect: stop hair loss, black hair grows back in the hairless area; 2) Effective: hair loss is significantly improved, and black hair grows back in some hairless areas; 3) Slight effect: hair loss is improved, hairless area Veil grows; 4) Ineffective: No improvement or reduction in hair loss, no new hair grows in the hairless area. The results are shown in Table 2 below:

Table 2

effective efficient small effect invalid Efficiency % Example 4 18 2 3 1 75 Example 5 20 3 1 1 80 Example 6 twenty two 1 1 1 88 Comparative Example 1 15 4 3 3 60 Comparative Example 2 8 8 5 4 32 Comparative Example 3 16 5 3 1 64

It can be seen from the above table 2 that the hair growth solution prepared by the present invention has remarkable effect when used in clinic and has high practicability.

The hair growth liquid prepared by Example 5 and Comparative Examples 4-7 was tested for the hair growth status of C57BL6 mice, and the results were shown in Table 3 below:

laboratory animal

7-week-old C57BL6BL6 mice, male, SPF grade, 15-20g, 70 mice. The temperature was 22-25°C, normal light, and the animals were fed with standard pelleted rat feed.

(1) Experimental grouping

70 mice were adaptively fed for 1 week in an air-conditioned room, with free access to water and natural light, and then randomly divided into a normal group (smeared with 75% ethanol by volume) and a model group (smeared with 100 μL of 0.05% testosterone solution) ) Example 5 group, comparative example 4 group, comparative example 5 group, comparative example 6 group, comparative example 7 group, a total of 6 groups, 10 in each group.

(2) Establishment of animal models

One day before the experiment, the mice were anesthetized with ether, heated and melted with a rosin/paraffin mixture (1:1), and then applied to an area of about 2 cm×2.5 cm on the back of C57BL6 mice. The resting period enters the growing period.

(3) Drug treatment

On the second day of depilation, except for the normal group, 100 μL of 0.05% testosterone solution (0.05g of testosterone dissolved in 100mL of 75% ethanol solution) was applied to the back of the mice in the other groups except the normal group, once a day for 28 consecutive days. Testosterone solution treatment After 30 minutes, apply 100 μL of hair tonic solution to the hair removal area in the same way, once a day for 28 consecutive days.

After depilation, the skin of 7-week-old mice turned pink, and the hair entered the telogen phase. The time when the skin color of the mice changed from pink to black and the time of long hair were observed and recorded every day.

table 3

The table arranges the model group adjustments below the normal group

As can be seen from the above table, the embodiment of the present invention is compared with the normal group and the finasteride control group (Comparative Example 4), the mouse skin discoloration time is shorter, and the hair growth time is also greatly shortened, which can effectively control the effect of testosterone on hair follicles. Negative Effects.

The above content is only an example and description of the concept of the present invention. Those skilled in the art can make various modifications or supplements to the described specific embodiments or replace them in a similar manner, as long as they do not deviate from the concept of the invention. Or beyond the scope defined by the claims, all belong to the protection scope of the present invention.

Claims (6)

1. A hair growth liquid for preventing seborrheic alopecia and promoting hair growth, characterized in that it comprises the following raw materials by weight: 0.005-0.03% acetyl tetrapeptide-3, 0.01-0.1% myristoyl pentapeptide-17, 0.005-0.025% Biotin 3-Peptide-1, 1-6% Helichrysum extract, 1-6% Platycladus orientalis leaf extract, 0.2-2% estrogen Chinese medicine extract, 0.2-2% Panthenol, 0.2-0.5% phenoxyethanol, 0.5-3% cornflower extract, 0.01-0.02% fullerene derivative, and the balance is alcohol-water buffer. 2. a kind of hair growth liquid that prevents seborrheic alopecia and promotes hair growth according to claim 1, is characterized in that, described fullerene derivative comprises the following steps to make: Step S1, add carbon 60 into the three-necked flask, slowly add liquid bromine dropwise, stir magnetically for 72-96 hours at room temperature, filter under reduced pressure after the reaction, put the filter cake at room temperature for 24 hours, and then vacuum dry it at 60 °C for 10 hours , the modified fullerene is prepared, and the dosage ratio of carbon 60 and liquid bromine is controlled to be 400-500 mg: 100 mL; Step S2, adding the modified fullerene into anhydrous ethanol, stirring magnetically for 30 minutes, adding 13% aqueous sodium hydroxide solution dropwise, stirring at a constant speed at room temperature and reacting for 6 hours, after the reaction, add concentrated hydrochloric acid dropwise to adjust pH, until pH= 5-5.6, centrifuge, collect the supernatant, spin the supernatant at 45°C, extract the spin-evaporated product with acetone, repeat three times to obtain a crude product, purify, obtain a fullerene derivative, and control the modification to enrich Lenene, absolute ethanol and aqueous sodium hydroxide solution 200-250mg: 25-30mL: 40mL. 3. a kind of hair growth liquid for preventing seborrheic alopecia and promoting hair growth according to claim 1, is characterized in that, described immortelle extract, arborvitae leaf extract, estrogen class Chinese medicine extract and cornflower extract The product includes the following steps: Helichrysum, oriental orientalis leaves, estrogen-based Chinese herbal medicines and cornflower were placed in an oven, dried at 40-45 ° C for 2 hours, and then the volume fraction of 50% ethanol aqueous solution was used as the extraction solvent, according to the ratio of material to liquid 1: : The ratio of 10 was added into a sealed container, soaked at 60 ° C for 2 hours, then kept warm and ultrasonicated at 40 Hz for 2 hours, and filtered to obtain Helichrysum extract, Platycladus orientalis leaf extract, estrogenic Chinese medicine extract and cornflower respectively. Extract. 4. a kind of hair growth liquid that prevents seborrheic alopecia and promotes hair growth according to claim 3, is characterized in that, described estrogen class Chinese herbal medicine is any one or more in Astragalus, Sophora flavescens and Ligustrum lucidum. Mixed in any proportion. 5. a kind of hair growth liquid that prevents seborrheic alopecia and promotes hair growth according to claim 1, is characterized in that, described alcoholic water buffer is 1,3-propylene glycol and deionized water according to the weight of 1: 50-60 than mixed. 6. a kind of preparation method of preventing seborrheic alopecia and promoting the hair growth liquid of hair growth according to claim 1, is characterized in that, comprises the steps: Acetyl tetrapeptide-3, myristoyl pentapeptide-17, biotin 3-peptide-1, Helichrysum extract, Platycladus orientalis leaf extract, estrogenic Chinese medicine extract, panthenol, phenoxyethanol, arrowroot The chrysanthemum extract and the fullerene derivative are mixed and shaken by vortex for 15-30 minutes, and then an alcohol-water buffer is added to prepare a hair growth solution for preventing seborrheic alopecia and promoting hair growth.