CN115176608B - Rapid seedling method for dendrobium candidum stem node induced buds - Google Patents
Rapid seedling method for dendrobium candidum stem node induced buds Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N39/00—Biocides, pest repellants or attractants, or plant growth regulators containing aryloxy- or arylthio-aliphatic or cycloaliphatic compounds, containing the group or, e.g. phenoxyethylamine, phenylthio-acetonitrile, phenoxyacetone
- A01N39/02—Aryloxy-carboxylic acids; Derivatives thereof
- A01N39/04—Aryloxy-acetic acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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Abstract
The invention belongs to the technical field of bioengineering, and discloses a rapid seedling method for dendrobium candidum stem node induced buds, which comprises the steps of selecting a dendrobium candidum plant which is free of plant diseases and insect pests, enters a flowering period, is strong in plant growth, has a height of 30-40 cm and has 10-15 internodes, preparing a medicament, scratching, cutting, dipping, smearing and sealing the dendrobium candidum stem node by applying a plant biological promotion technology, and obtaining a root bud integrated plant on the dendrobium candidum stem node, and flowering on a mother plant the next year. The invention improves the propagation coefficient of the dendrobium candidum, has the advantages of high germination rate, quick seedling formation, good quality of small seedlings, strong growth vigor, shortened reproductive period, flowering in the next year and the like, can obtain the dendrobium candidum seedlings with high quality and uniformity in a short time, and provides an effective way for the breeding, seedling cultivation, standardization, commercial production and species protection of the dendrobium candidum and the sustainable utilization of orchid plants.
Description
Technical Field
The invention belongs to the technical field of bioengineering, and particularly relates to a rapid seedling method for dendrobium candidum stem node induction buds.
Background
At present, dendrobium candidum (Dendrobium officinale Kimura et migo.) belongs to a perennial epiphyte of Dendrobium (Dendrobium SW.) in Orchidaceae, is grown on rocks with the altitude of 1600 meters and the mountain area with semi-yin dampness, is warm and moist and environment with semi-yin and semi-yang, is not cold-resistant, has the effects of nourishing yin, clearing heat, benefiting stomach, promoting salivation, moistening lung, relieving cough, improving digestive system and the like, is often used for treating various diseases such as body fluid impairment due to heat, dry mouth, polydipsia, deficiency heat after illness, weak eyesight and the like, is a traditional rare Chinese medicinal material in China, and has high medicinal value and market prospect.
The dendrobium candidum breeding foundation research is lack, blind introduction and seed source confusion, and in the multi-generation tissue culture propagation process, the phenomena of serious variety degradation and vitrification are caused by continuous subculture, so that the variety breeding and seedling recovery are required to be carried out.
Under the natural environment condition, different cultivation modes are adopted, the dendrobium candidum produces various genetic variation types, the specificity of gardening characters and agronomic characters is obvious, the resource quality is good and uneven, the difference of medicinal components is large, the requirements of consumers on high-quality dendrobium candidum products cannot be met, and the dendrobium candidum good varieties suitable for local habitat and climate characteristics, namely the development excavation of local seed sources, are urgently required to be cultivated in production.
In recent years, a dendrobium nobile research and development team in the Guizhou forest sciences relies on projects such as "Guizhou dendrobium nobile plant germplasm resource library" and "dendrobium candidum imitative wild test cultivation test planting base", a seed source, a epiphytic tree seed, a binding planting season, a binding planting height, an altitude, a binding planting belt type, a binding planting moss, a forest land cleaning or not, fertilization, watering, drug administration and other different test designs are carried out on dendrobium candidum cultivated by the epiphytic tree, and the dendrobium nobile cultivation method aims at cultivating dendrobium candidum excellent varieties suitable for different planting modes.
The breeding and the good variety cultivation of the dendrobium candidum are the primary tasks of the current industry development, the dendrobium candidum is an orchid epiphyte which is slow in growth and low in self-reproductive capacity, seeds are difficult to reproduce and can germinate only by symbiotic with fungi, and the traditional modes of dividing plants, cutting and the like have low reproduction coefficients, usually 1-3, so that the development of asexual rapid reproduction related technology is carried out by using the bred good dendrobium candidum branches as materials, the growth period is short, the blooming is advanced, the requirement of high yield is met, the rapid reproduction of the good variety is achieved, a quality-controllable breeding technology system is formed, and the good variety is provided for the industrialized development of the dendrobium candidum.
Through the above analysis, the problems and defects existing in the prior art are as follows:
(1) Dendrobium officinale seeds are difficult to reproduce, and the traditional modes of dividing plants, cutting and the like have low reproduction coefficients.
(2) The dendrobium candidum breeding foundation research is lack, the seed is introduced blindly, the seed source is disordered, and in the multi-generation tissue culture propagation process, the variety degradation and vitrification phenomenon are serious due to continuous subculture.
(3) Under the natural environment condition, the dendrobium candidum has various genetic variation types, obvious gardening character and agronomic character specificity, good resource quality and high medicinal effect component difference, and cannot meet the requirements of consumers on high-quality dendrobium candidum products.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention provides a rapid seedling method of dendrobium candidum stem node induced buds, and particularly relates to application of a mixed medicament with higher biological promotion of dendrobium candidum bud seedling.
The invention discloses a rapid seedling method of dendrobium candidum stem node induced buds, which comprises the following steps: through screening of good plants of dendrobium candidum, artificial incision and other treatments are carried out among each section, and a mixed reagent I and a mixed reagent II for inducing bud germination and rooting are respectively applied, so that a large number of healthy and uniform seedlings are obtained in a short time.
Further, the rapid seedling method of the dendrobium candidum stem node induction bud comprises the following steps:
step one, establishing a dendrobium candidum culture object: selecting a strong plant of dendrobium candidum without plant diseases and insect pests which grows in a pot plant as a test material, and carrying out pretreatment before experiments;
step two, stem segment treatment: selecting a dendrobium candidum stem with a certain number of internodes on a dendrobium candidum plant which grows robustly, flushing the dendrobium candidum stem with a certain number of internodes by using a shower nozzle, sterilizing the dendrobium candidum plant with alcohol, cutting a notch with a certain depth at each internode of the stem by using a sterile scalpel, and cutting the notch by adopting the same method at the position of the opposite side below the stem to apply mixed medicament;
step three, induction bud stage: in order to obtain more dendrobium candidum sprouts, a dropper is used for sucking a mixed medicament I of 6-benzyl aminopurine (6-BA) and sialon (TDZ) with a certain concentration, the mixed medicament I is dripped on a notch between each node, paraffin is used for sealing, and the dendrobium candidum sprouts are cultured for 20-30 days and differentiated into radicle-free buds;
step four, induction rooting stage: in order to obtain dendrobium candidum plants which grow independently, a dropper is used for sucking a mixed medicament II of 2, 4-dichlorophenoxyacetic acid (2, 4-D) and alpha-naphthylacetic acid (NAA) with a certain concentration, the mixed medicament II is dripped on an opposite side incision of each internode, paraffin is used for sealing, and the whole plants with 2 to 3 roots are grown after 10 to 20 days of culture;
hardening seedlings: when dendrobium candidum seedlings grow to a certain height, peeling the rooted seedlings from the base, soaking the rooted seedlings in thiophanate-methyl solution with a certain dilution for a period of time, taking the rooted seedlings out of a greenhouse by forceps, and airing the rooted seedlings in the greenhouse to avoid root rot;
step six, transplanting: when the root is dehydrated and whitened, planting the dendrobium candidum into a seedling raising tray which is sterilized in advance and is a mixed matrix composed of sawdust and perlite, and normally growing the dendrobium candidum under proper conditions; after transplanting survival, the growing season is more in watering and applying, the thin fertilizer is applied frequently, the pest management and protection are enhanced, and the dendrobium candidum flowers in the next year, so that the flowering time is shortened, and the requirements of commercial good seedlings are met in a short time.
Further, the rapid seedling method of the dendrobium candidum stem node induction bud further comprises the following steps:
separating seedling by peeling rhizome, planting on seedling tray containing matrix, and obtaining a large number of regular and uniform flowering plants of Dendrobium officinale under certain cultivation environment conditions in short time.
Further, the establishing of the dendrobium candidum culture object in the step one includes:
selecting a dendrobium candidum plant which is cultivated in a pot, grows well and has strong growth vigor, high content of medicinal components and no plant diseases and insect pests as a mother plant, placing the dendrobium candidum plant into a greenhouse with 70 percent sunshade, picking terminal buds of all branches of the dendrobium candidum within 30 to 40 days before flower bud differentiation, removing top end advantages, inhibiting high growth, and obtaining the culture material.
Further, the processing of the stem segment in the second step includes:
for the screened dendrobium candidum plants, dendrobium candidum stems with the height of 20-30 cm and 10-15 internodes are selected, the dendrobium candidum stems are washed for 5min by a shower nozzle, each internode is sterilized by dipping a cotton swab in 75% alcohol, a notch is formed between the internodes by using a sterile scalpel, the depth is 1mm, and the notch with the same depth is also formed under the opposite side by the same method.
Further, the mass ratio of the 6-benzyl aminopurine to the sialon in the third step is 1:1, wherein the concentration of the mixed medicament I is 1.0mg/L; culturing for 20-30 days, and differentiating 1-2 strains on the node.
Further, the mass ratio of 2,4 dichlorophenoxyacetic acid to alpha-naphthylacetic acid in the fourth step is 1:1, wherein the concentration of the mixed medicament II is 0.5mg/L; culturing for 10-20 days to obtain a complete plant with 2-3 roots and 3-4 leaves.
Further, the seedling hardening in the fifth step includes:
when the dendrobium candidum seedlings grow to 8-10 cm and have 4-6 roots, the rooted seedlings are peeled off from the base, are soaked in 1500 times of 50% thiophanate-methyl wettable powder for 3-5 min, and are dried in a greenhouse for 2-3 days.
Further, the transplanting in the step six includes:
after the root is dehydrated and whitened, planting the plant to be sterilized by 50% carbendazim 500 times liquid in advance, wherein the volume ratio of sawdust to perlite is 3:1, planting 120 plants in each seedling tray of the mixed matrix, watering enough root fixing water, placing the seedling tray in a greenhouse, keeping the temperature of 20-28 ℃, the humidity of 60% and the sun-shading and ventilation conditions of 70%, and enabling dendrobium candidum to grow normally, wherein the transplanting survival rate of the seedlings reaches more than 98%; the flowers which are diluted 1000 times are more when the plant is watered once in 10-15 days in the growing season, the thin fertilizer is applied frequently, and the management and the protection of the plant diseases and insect pests are enhanced.
The invention further aims to provide a mixed medicament for promoting the sprouting and rooting of the dendrobium candidum, which is used for implementing the rapid seedling method of the dendrobium candidum stem node induced buds, wherein the mixed medicament for promoting the sprouting and rooting of the dendrobium candidum comprises a mixed medicament I and a mixed medicament II.
Wherein the mixed medicament I is prepared from 6-benzyl aminopurine and sialon according to the weight ratio of 1:1, the concentration is 1.0mg/L, and the dendrobium officinale bud induction agent is used in the dendrobium officinale bud induction stage;
the mixed medicament II is prepared from 2,4 dichlorophenoxyacetic acid and alpha-naphthylacetic acid according to the proportion of 1:1, the concentration is 0.5mg/L, and the dendrobium officinale root is used for inducing rooting.
In combination with the above technical solution and the technical problems to be solved, please analyze the following aspects to provide the following advantages and positive effects:
first, aiming at the technical problems in the prior art and the difficulty in solving the problems, the technical problems solved by the technical proposal of the invention are analyzed in detail and deeply by tightly combining the technical proposal to be protected, the results and data in the research and development process, and the like, and some technical effects brought after the problems are solved have creative technical effects. The specific description is as follows:
the dendrobium candidum has the advantages of medium and medium plants, she Er columns, long needle-like shape, compact plant type, inflorescence growing 3-5 flowers, upright stem, red, cylindrical shape, similar thickness of the stem up and down, obvious black knots, concave knots, slight fatness among knots, high polysaccharide content, thick sticky and greasy feeling after chewing, less residues, difficult lodging, low temperature resistance of-4 ℃ and the like, and adopts plant biological promotion technology, namely a rapid seedling forming method combining scratch and incision among the knots with co-stimulation of the mixed medicament I and the mixed medicament II, a large number of root buds are germinated on the knots in a short period of time, so that robust and high-quality seedlings flowering in the next year are obtained, the requirements of breeding and commercialized markets are met, and the dendrobium candidum has great value in scientific research and development.
Secondly, the technical scheme is regarded as a whole or from the perspective of products, and the technical scheme to be protected has the following technical effects and advantages:
the invention develops the application of the mixed reagent I and the mixed reagent II for promoting the bred dendrobium candidum stem node induced bud seedling with strong growth vigor, high content of medicinal components and high stress resistance.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments of the present invention will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a flowchart of a rapid seedling method of dendrobium candidum stem node induction buds provided by an embodiment of the invention;
fig. 2 to 3 are schematic diagrams of induction roots and buds of dendrobium candidum stem nodes provided by the embodiment of the invention.
Detailed Description
The present invention will be described in further detail with reference to the following examples in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
Aiming at the problems existing in the prior art, the invention provides a rapid seedling method of dendrobium candidum stem node induced buds, and the invention is described in detail below with reference to the accompanying drawings.
1. The embodiments are explained. In order to fully understand how the invention may be embodied by those skilled in the art, this section is an illustrative embodiment in which the claims are presented for purposes of illustration.
As shown in fig. 1, the rapid seedling method for dendrobium candidum stem node induction buds provided by the embodiment of the invention comprises the following steps:
s101, establishing a dendrobium candidum culture object: selecting a strong plant of dendrobium candidum without plant diseases and insect pests in a potted plant as a test material, and carrying out pretreatment before experiments;
s102, stem segment treatment: selecting dendrobium candidum stems from the dendrobium candidum plants which grow robustly, washing, sterilizing, scoring incisions at each internode of the stems, and treating opposite side deflection by adopting the same method;
s103, induction bud stage: sucking a mixed medicament I consisting of 6-benzyl aminopurine and sialon by using a dropper, dripping the mixed medicament I on a notch of each internode, sealing the notch by using paraffin, culturing for a period of time, and differentiating into small buds;
s104, induction rooting phase: a mixed reagent II consisting of 2,4 dichlorophenoxyacetic acid and alpha-naphthylacetic acid is sucked by a dropper, and is dripped on a notch at the lower side of the opposite side, sealed and cultivated to grow into a complete plant;
s105, hardening: when dendrobium candidum seedlings grow to a certain height, peeling the rooted seedlings from the base, soaking the rooted seedlings in thiophanate-methyl solution with a certain dilution, taking out the dendrobium candidum seedlings, and airing the dendrobium candidum seedlings in a greenhouse;
s106, transplanting: and (3) planting the dendrobium candidum into a seedling raising tray which is subjected to disinfection treatment in advance and is composed of saw dust and perlite after the root is dehydrated and whitened, planting, managing and flowering the dendrobium candidum in the next year.
Schematic diagrams of induction roots and buds of dendrobium candidum stems provided by the embodiment of the invention are shown in figures 2-3.
Example 1
The invention develops the application of the mixed medicament for promoting the screened dendrobium candidum stem node induced bud seedling with strong growth vigor, high content of medicinal components and high stress resistance. Selecting a dendrobium candidum plant which is free of plant diseases and insect pests, enters a flowering period, is strong in plant growth, has a height of 30-40 cm and has 10-15 internodes, and is subjected to the operation steps of preparation of a medicament, internode scratch incision, dipping, smearing, sealing and the like by applying a plant biological promotion technology, so that a root and bud integrated plant is obtained on a stem node of the dendrobium candidum, flowers on a mother plant the next year, then, by rhizome stripping, separated seedlings are planted on a seedling raising tray containing a matrix, certain cultivation environmental conditions are given in a short time, and a large number of uniform dendrobium candidum flowering plants can be obtained.
The rapid seedling method of dendrobium candidum stem node induced buds provided by the embodiment of the invention comprises the following steps:
(1) Establishment of dendrobium candidum culture objects: selecting a dendrobium candidum plant which is cultivated in a pot, grows well and has strong growth vigor, high content of medicinal components and no plant diseases and insect pests as a mother plant, placing the dendrobium candidum plant into a greenhouse with 70 percent sunshade, picking terminal buds of all branches of the dendrobium candidum within 30 to 40 days before flower bud differentiation, removing top end advantages, inhibiting high growth, and obtaining the culture material.
(2) Stem segment treatment: for the obtained dendrobium candidum plants, dendrobium candidum stems with the height of 20-30 cm and 10-15 internodes are selected, the dendrobium candidum stems are washed for 5min by a shower nozzle, each internode is sterilized by dipping a cotton swab in 75% alcohol, a notch is formed between the stem nodes by a sterile scalpel, the depth is 1mm, and the notch with the same depth is also formed under the opposite side by the same method.
(3) Induction bud stage: in order to obtain more dendrobium candidum induced buds, a dropper is used for sucking a mixed solution (hereinafter referred to as mixed medicament I) of 1.0mg/L of 6-benzyl aminopurine (6-BA) and sialon (TDZ) (the mass ratio is 1:1), the mixed solution is dripped on a notch between each section of dendrobium candidum stem, paraffin is used for sealing, and the culture is carried out for 20-30 days, so that the number of differentiated buds on the sections reaches 1-2 strains.
(4) Induction rooting stage: in order to obtain dendrobium candidum plants which grow independently, a dropper is used for sucking mixed liquid (hereinafter referred to as mixed reagent II) of 0.5mg/L of 2, 4-dichlorophenoxyacetic acid (2, 4-D) and alpha-naphthylacetic acid (alpha-NAA) (the mass ratio is 1:1), the mixed liquid is dripped on a notch between each section on the opposite side, paraffin is used for sealing, and the whole plant with 2-3 roots and 3-4 leaves is grown after 10-20 days of culture.
(5) Hardening seedlings and transplanting: when the dendrobium candidum seedlings grow to 8-10 cm and have 4-6 roots, the rooting seedlings are gently peeled off from the base by hands, placed in 1500 times of 50% thiophanate-methyl wettable powder for soaking for 3-5 minutes, taken out by forceps and dried in a greenhouse for 2-3 days, planted into seedling trays which are sterilized by 500 times of 50% carbendazim and are prepared from saw dust and perlite (volume ratio is 3:1) in advance, planted with 120 plants, poured with root fixing water, placed in the greenhouse, kept at 20-28 ℃ and 60% humidity and 70% sunshade and ventilation conditions, and the dendrobium candidum can normally grow, and the transplanting survival rate of the seedlings reaches more than 98%. The growing season is usually 10-15 days, more flowers are watered once and diluted 1000 times, thin fertilizer is applied frequently, the pest management and protection are enhanced, and the dendrobium candidum can bloom in the next year.
The beneficial effects of the invention are as follows: the application of the biological promotion dendrobium candidum bud and root induction mixed medicament I and mixed medicament II is an efficient propagation method for obtaining a large number of dendrobium candidum buds to form seedlings, improves the propagation coefficient of the dendrobium candidum, has the advantages of high germination rate, quick seedling formation, good quality of small seedlings, strong growth vigor, shortened reproduction period, flowering in the next year and the like, can obtain high-quality and tidy dendrobium candidum seedlings in a short time, and provides an effective way for the breeding, seedling cultivation, standardization, commercial production and species protection of the dendrobium candidum and the sustainable utilization of orchid plants.
Example 2
The rapid seedling method of dendrobium candidum stem node induced buds provided by the embodiment of the invention comprises the following steps:
(1) Establishing a dendrobium candidum culture object: and selecting a strong plant of dendrobium candidum without plant diseases and insect pests in a potted plant as a test material, and carrying out pretreatment before experiments.
(2) Stem segment treatment: on a strong dendrobium candidum plant, a dendrobium candidum stem with a certain height and a certain number of internodes is selected, the dendrobium candidum stem is washed by a shower nozzle, the dendrobium candidum is sterilized by alcohol, a notch with a certain depth is formed at each internode of the stem by a sterile scalpel, and the opposite side is treated by the same method.
(3) Induction bud stage: in order to obtain more dendrobium candidum sprouts, a dropper is used for sucking 6-benzyl aminopurine (6-BA) and sialon (TDZ) (the mass ratio is 1:1) mixed medicament I with a certain concentration, the mixture is dripped on a notch between each section, paraffin is used for sealing, and the mixture is cultured for a period of time to differentiate into small buds.
(4) Induction rooting stage: in order to obtain dendrobium candidum plants which grow independently, 2, 4-dichlorophenoxyacetic acid (2, 4-D) and alpha-naphthylacetic acid (NAA) (the mass ratio is 1:1) mixed agent II with a certain concentration are sucked by a dropper, the mixed agent II is dripped on a notch on the opposite side and is sealed by paraffin, and the whole plant is grown after a period of culture.
(5) Hardening seedlings and transplanting: when dendrobium candidum seedlings grow to a certain height, lightly stripping the rooted seedlings from the base by hands, soaking the rooted seedlings in thiophanate-methyl solution with a certain dilution, taking out the rooted seedlings from a greenhouse by forceps, airing the rooted seedlings in the greenhouse, planting the rooted seedlings into seedling trays which are subjected to disinfection treatment in advance and are formed by saw dust and perlite (volume ratio is 3:1), planting 120 plants on each tray, watering enough rooting water, and placing the seedling trays in the greenhouse. The dendrobium candidum can grow normally with the transplanting survival rate of the seedlings reaching more than 98 percent by keeping the temperature of 20-28 ℃ and the humidity of 60 percent and shading and ventilating at 70 percent, the 1000 times diluted flowers are more, the thin fertilizer is applied frequently after 10-15 days in the growing season, the pest management and protection are enhanced, and the dendrobium candidum can bloom in the next year.
2. Application example. In order to prove the inventive and technical value of the technical solution of the present invention, this section is an application example on specific products or related technologies of the claim technical solution.
The application of the biological promotion dendrobium candidum bud and root induction mixed medicament provided by the embodiment of the invention is an efficient propagation method for obtaining a large number of dendrobium candidum buds, improves the propagation coefficient of dendrobium candidum, has the advantages of high germination rate, quick seedling formation, good quality of small seedlings, shortened vegetative growth period, flowering in the next year and the like, can obtain dendrobium candidum seedlings with high quality and uniformity in a short time, and provides an effective way for dendrobium candidum breeding, seedling cultivation, standardization, commercial production and species protection and sustainable utilization of orchid plants.
3. Evidence of the effect of the examples. The embodiment of the invention has a great advantage in the research and development or use process, and has the following description in combination with data, charts and the like of the test process.
TABLE 1 Dendrobium officinale Mixed preparation I and Mixed preparation II induce bud and root germination rates (see Table below)
As can be seen from Table 1, for 52 internodes of selected Dendrobium officinale, 1mm is cut at one side of the internode, mixed agent I is implemented and treated, after 15 days, small buds with the length of about 1cm are differentiated on each node, after 22 days, statistics is carried out, the small buds reach 15-20 cm, the number of the small buds is 87, the differentiation rate of the induced buds can reach 167%, namely, 1-2 buds can be induced on average in each node; also, 1mm is cut at the opposite side and is treated by a mixed medicament II, roots with the length of about 6 cm to 10cm are differentiated on each section after 8 days, the root length is 12 cm to 18cm after 18 days, the number of 193 roots is counted, the differentiation rate of the induced roots can reach 371%, namely, 2 to 3 roots or more can be induced on average per section; under the condition that the control group is carried out without a notch or a mixed medicament, only 11 buds are induced among 52 internodes, the buds induce rooting of 23 buds, and the induction rate of the buds and roots is less than 50%.
The foregoing is merely illustrative of specific embodiments of the present invention, and the scope of the invention is not limited thereto, but any modifications, equivalents, improvements and alternatives falling within the spirit and principles of the present invention will be apparent to those skilled in the art within the scope of the present invention.
Claims (8)
1. The rapid seedling method of the dendrobium candidum stem node induced buds is characterized by comprising the following steps of:
step one, establishing a dendrobium candidum culture object: selecting a strong plant of dendrobium candidum without plant diseases and insect pests which grows in a pot plant as a test material, and carrying out pretreatment before experiments;
step two, stem segment treatment: selecting a dendrobium candidum stem with a certain number of internodes on a dendrobium candidum plant which grows robustly, flushing the dendrobium candidum stem with a certain number of internodes by using a shower nozzle, sterilizing the dendrobium candidum plant with alcohol, drawing a notch with a certain depth at each internode of the stem by using a sterile scalpel, and treating the opposite side downward by adopting the same method;
step three, induction bud stage: in order to obtain more dendrobium candidum sprouts, a dropper is used for sucking a mixed medicament I of 6-benzyl aminopurine and sialon with a certain concentration, the mixed medicament I is dripped on a notch between each node, paraffin is used for sealing, and the mixed medicament I is cultured for a period of time and differentiated into small buds;
step four, induction rooting stage: in order to obtain an independently grown dendrobium candidum plant, a dropper is used for sucking mixed reagent II of 2, 4-dichlorophenoxyacetic acid and alpha-naphthylacetic acid with a certain concentration, the mixed reagent II is dripped on a notch on the opposite side, a paraffin is used for sealing, and a period of time is used for culturing to grow a complete plant;
hardening seedlings: when dendrobium candidum seedlings grow to a certain height, peeling the rooted seedlings from the base, soaking the rooted seedlings in thiophanate-methyl solution with a certain dilution, taking the rooted seedlings out of the greenhouse by forceps, and airing the rooted seedlings in the greenhouse;
step six, transplanting: planting the dendrobium candidum into a seedling tray which is sterilized in advance and is a mixed matrix composed of sawdust and perlite after the root is dehydrated and whitened; after the seedlings are transplanted to survive, the growing season is that more flowers are poured, the thin fertilizer is applied frequently, the pest management and protection are enhanced, and the dendrobium candidum flowers in the next year.
2. The method for rapid seedling formation of dendrobium candidum stem node-induced buds as claimed in claim 1, wherein the establishment of the dendrobium candidum culture object in the step one comprises:
selecting a dendrobium candidum plant which is cultivated in a pot, grows well and has strong growth vigor, high content of medicinal components and no plant diseases and insect pests as a mother plant, placing the dendrobium candidum plant into a greenhouse with 70 percent sunshade, picking terminal buds of all branches of the dendrobium candidum within 30 to 40 days before flower bud differentiation, removing top end advantages, inhibiting high growth, and obtaining the culture material.
3. The rapid seedling method of dendrobium candidum stem node induced buds of claim 1, wherein the stem segment treatment in the second step comprises:
for the obtained dendrobium candidum plants, dendrobium candidum stems with the height of 20-30 cm and 10-15 internodes are selected, the dendrobium candidum stems are washed for 5min by a shower nozzle, each internode is sterilized by dipping a cotton swab in 75% alcohol, a notch is formed in each internode by a sterile scalpel, the depth is 1mm, and the notch with the same depth is formed in the position of the opposite side in a similar method.
4. The rapid seedling method of dendrobium candidum stem node induction buds of claim 1, wherein the mass ratio of 6-benzyl aminopurine to sialon in the third step is 1:1, wherein the concentration of the mixed medicament I is 1.0mg/L; culturing for 20-30 days, and differentiating 1-2 strains on the node.
5. The rapid seedling method of dendrobium candidum stem node induction buds of claim 1, wherein the mass ratio of 2,4 dichlorophenoxyacetic acid to alpha-naphthylacetic acid in the fourth step is 1:1, wherein the concentration of the mixed medicament II is 0.5mg/L; culturing for 10-20 days to obtain a complete plant with 2-3 roots and 3-4 leaves.
6. The method for rapid seedling formation of dendrobium candidum stem node-induced buds as claimed in claim 1, wherein the seedling hardening in the fifth step comprises:
when the dendrobium candidum seedlings grow to 8-10 cm and have 4-6 roots, the rooted seedlings are peeled off from the base, are soaked in 1500 times of 50% thiophanate-methyl wettable powder for 3-5 min, and are dried in a greenhouse for 2-3 days.
7. The method for rapid seedling formation of dendrobium candidum stem node-induced buds as claimed in claim 1, wherein the transplanting in the step six comprises:
after the root is dehydrated and whitened, planting the plant to be sterilized by 50% carbendazim 500 times liquid in advance, wherein the volume ratio of sawdust to perlite is 3:1, planting 120 plants in each seedling tray of a mixed matrix, watering enough root fixing water, placing the seedling tray in a greenhouse, keeping the temperature of 20-28 ℃, 60% humidity and 70% sun-shading and ventilation conditions, and enabling dendrobium candidum to grow normally, wherein the transplanting survival rate of the seedlings reaches more than 98%; the flowers which are diluted 1000 times are more when the plant is watered once in 10-15 days in the growing season, the thin fertilizer is applied frequently, and the management and the protection of the plant diseases and insect pests are enhanced.
8. A mixed medicament for promoting dendrobium candidum induced buds and roots for implementing the rapid seedling method of dendrobium candidum stem node induced buds according to any one of claims 1-7, which is characterized in that the mixed medicament for promoting dendrobium candidum buds and roots comprises a mixed medicament I and a mixed medicament II;
wherein the mixed medicament I is prepared from 6-benzyl aminopurine and sialon according to the weight ratio of 1:1, the concentration is 1.0mg/L, and the dendrobium officinale bud induction agent is used in the dendrobium officinale bud induction stage;
the mixed medicament II is prepared from 2,4 dichlorophenoxyacetic acid and alpha-naphthylacetic acid according to the proportion of 1:1, the concentration is 0.5mg/L, and the dendrobium officinale rooting agent is used for the induction rooting stage of dendrobium officinale.
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| CN103155871A (en) * | 2013-03-07 | 2013-06-19 | 华中科技大学 | Dendrobium officinale sprout rapid propagation method with high efficiency |
| CN106106103A (en) * | 2016-07-11 | 2016-11-16 | 广西壮族自治区林业科学研究院 | Scribbling sketch Herba Dendrobii old stem cuttage breeding method |
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| CN103155871A (en) * | 2013-03-07 | 2013-06-19 | 华中科技大学 | Dendrobium officinale sprout rapid propagation method with high efficiency |
| CN106106103A (en) * | 2016-07-11 | 2016-11-16 | 广西壮族自治区林业科学研究院 | Scribbling sketch Herba Dendrobii old stem cuttage breeding method |
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