CN115152835A - Method for preserving Dictyophora rubrovalvata by using melatonin - Google Patents

Abstract

The invention discloses a fresh-keeping method for Pseudomonas erythraea by utilizing melatonin, which comprises the following steps: removing the roots, removing the mud, and cutting off the outer epidermis of the fungus caps from the freshly picked Bamboo spp. The latter is put into sterile water for soaking, and after soaking is taken out and quickly removes the surface moisture of the rhizoma rhododendron by a fan; the rhododendron rhododendron treated in S2 is put into a sterile pool with a preservative. Soaking, the beneficial effects of the present invention are: by adding a preservative soaking under the traditional low-temperature, sealing and nitrogen-filled preservation methods, the preservation effect of Pseudomonas serrata can be improved, and at the same time, melatonin is widely present in the plant. Indole plant hormones, as an antioxidant, can directly scavenge reactive oxygen species, activate antioxidant enzymes, and delay the aging of fruits and vegetables by scavenging free radicals, inhibiting ethylene synthesis, inducing protein oxidative damage repair enzymes, and regulating energy metabolism. Tolerance to abiotic and biotic stresses.

Description

A kind of fresh-keeping method of using melatonin for Bamboo fungus rhododendron

technical field

The invention relates to the technical field of fresh-keeping of Bamboo spp., in particular to a fresh-keeping method for Bamboo scutellariae serrata using melatonin.

Background technique

Bamboo fungus Hongtuo belongs to the genus Bamboo fungus of the Phalloidinaceae family and belongs to porous edible fungi. It is rich in polysaccharides and amino acids, and has antioxidant, anticancer, and hypoglycemic effects. Fresh vegetables and fruits are very beneficial to human health. Therefore, With the upgrading of consumption concepts and the demand for fresh Bamboo fungus hongtuo products in the consumer market, the market sales ratio of fresh Bamboo fungus hongtuo products will increase day by day. Effective protection of tissues and fruit bodies with high water content, frequent occurrence of postharvest water loss, autolysis, and decay, which affects the value of commodities. The existing preservation methods are nitrogen-filled, sealed at low temperature and chemical agents, although they can play a certain role. However, it is impossible to accurately control the content of chemical agents during operation, which may easily lead to chemical residues, thus causing contamination to Bamboo fungus.

SUMMARY OF THE INVENTION

The object of the present invention is to provide a fresh-keeping method for Pseudomonas serrata using melatonin to solve the problems raised in the above-mentioned background technology.

In order to achieve the above object, the present invention provides the following technical solutions: a kind of method for preserving the freshness of Bamboo Radix with melatonin, comprising the following steps:

S1, remove the root, remove the mud, and cut off the outer epidermis of the fungus cap from the freshly plucked Radix Rhododendron;

S2, put the bamboo fungus rhododendron treated in S1 into sterile water for soaking, take out after soaking and quickly remove the surface moisture of the bamboo fungus rhododendron rhododendron through the blower;

S3, put into the aseptic pool with antistaling agent and soak the treated in S2, and seal the aseptic pool with plastic wrap, until reaching the soaking time, take out the bamboo fungus of the red leaf;

S4. Put the fished out rhododendron into a fresh-keeping bag and seal it, and store it in a dark and constant temperature environment.

Preferably, the time for soaking in sterile water is 10S-15S in the S2.

As preferably, the preservative in described S3 comprises the raw material of following weight portion:

10-12 parts of water, 8-10 parts of melatonin, 3-5 parts of natamycin, 5-8 parts of ascorbic acid extract, 1-2 parts of ammonium bicarbonate and 2-3 parts of carboxymethyl chitosan;

The preparation method of the fresh-keeping liquid comprises the following steps:

A1. Take a container of corresponding capacity according to the formula and carry out disinfection and sterilization treatment, then take water, and pour one-half of the weighed water into the container, then weigh the ascorbic acid extract according to the formula, and pour the ascorbic acid extract into the container. In, water and ascorbic acid extract are mixed by glass rod to obtain stock solution a;

A2. Weigh out the corresponding doses of melatonin and natamycin according to the formula. After the water in the container and the ascorbic acid extract are completely fused, add melatonin and natamycin to the inside of the container, and then add the remaining water in A1. One half is added into the container, and the container is heated. During the heating process, the raw liquid a1 is obtained by continuous mixing with a glass rod. After the temperature of a1 in the container reaches the design standard, the heating is stopped;

A3. Weigh ammonium bicarbonate and carboxymethyl chitosan according to the formula. After the temperature of a1 is cooled to normal temperature, add the remaining water, ammonium bicarbonate and carboxymethyl chitosan into the container, and stir and mix again. The preservative can be prepared after ammonium bicarbonate and carboxymethyl chitosan are completely dissolved in the stock solution a1 and filtered through a filter screen.

As preferably, the preparation method of described ascorbic acid extract comprises the following steps:

B1. Peel the lemons, and put the skins into the debittering solution for debittering treatment to obtain the skin extracts, crush the lemon pulp with a juicer, and squeeze out the lemon juice in the pulp through filter paper, Mix the leather extract with lemon juice to obtain a stock solution;

B2. The vitamin extract is added to the stock solution, and the ascorbic acid extract can be obtained through stirring, filtration and separation processing.

As preferably, the preparation method of described melatonin comprises the steps:

C1. Take the plant stem diameter for cleaning, pulverize the cleaned stem diameter, then pour the crushed stem diameter into a centrifuge and add an appropriate amount of hot water, and let it stand after centrifugal processing by the centrifuge;

C2, take out the supernatant liquid sent by centrifuge and filter, add propylene glycol to the supernatant liquid after filtering and carry out extraction process, obtain extract;

C3. Pour the extract into a beaker for heating, so that the propylene glycol in the extract is evaporated to obtain melatonin crystals, and then the melatonin crystals are dried and ground to obtain the melatonin.

Preferably, the time for soaking in the antistaling agent of S. rhododendron in the S3 is 30-35 minutes.

Preferably, the concentration of nitrogen in the S4 fresh-keeping bag is 75%, the concentration of oxygen is 5%, and the concentration of carbon dioxide is 10%.

Preferably, the storage temperature in the S4 is 1-3°C.

Preferably, the concentration of the natamycin is 3.00ml/ml, and the concentration of the ascorbic acid extract is 7.00ml/ml.

Preferably, the temperature for heating a1 in the A2 is 30-35°C.

Compared with the prior art, the beneficial effects of the present invention are: by increasing the preservative soaking under the traditional low-temperature, sealing and nitrogen-filled preservation methods, the preservation effect of P. Indole phytohormones existing in plants, as an antioxidant, can directly scavenge reactive oxygen species, activate antioxidant enzymes, and delay fruits and vegetables by scavenging free radicals, inhibiting ethylene synthesis, inducing protein oxidative damage repair enzymes and regulating energy metabolism. senescence, thereby increasing the tolerance of fruits and vegetables to abiotic and biotic stresses.

Detailed ways

The invention provides a technical scheme: a fresh-keeping method for Pseudomonas erythraea by utilizing melatonin, comprising the following steps:

S1, remove the root, remove the mud, and cut off the outer epidermis of the fungus cap from the freshly plucked Radix Rhododendron;

S2, put the bamboo fungus rhododendron treated in S1 into sterile water for soaking, take out after soaking and quickly remove the surface moisture of the bamboo fungus rhododendron rhododendron through the blower;

S3, put into the aseptic pool with antistaling agent and soak the treated in S2, and seal the aseptic pool with plastic wrap, until reaching the soaking time, take out the bamboo fungus of the red leaf;

S4. Put the fished out rhododendron into a fresh-keeping bag and seal it, and store it in a dark and constant temperature environment.

Preferably, the time for soaking in sterile water is 10S-15S in the S2.

As preferably, the preservative in described S3 comprises the raw material of following weight portion:

10-12 parts of water, 8-10 parts of melatonin, 3-5 parts of natamycin, 5-8 parts of ascorbic acid extract, 1-2 parts of ammonium bicarbonate and 2-3 parts of carboxymethyl chitosan;

The preparation method of the fresh-keeping liquid comprises the following steps:

A1. Take a container of corresponding capacity according to the formula and carry out disinfection and sterilization treatment, then take water, and pour one-half of the weighed water into the container, then weigh the ascorbic acid extract according to the formula, and pour the ascorbic acid extract into the container. In, water and ascorbic acid extract are mixed by glass rod to obtain stock solution a;

A2. Weigh out the corresponding doses of melatonin and natamycin according to the formula. After the water in the container and the ascorbic acid extract are completely fused, add melatonin and natamycin to the inside of the container, and then add the remaining water in A1. One half is added into the container, and the container is heated. During the heating process, the raw liquid a1 is obtained by continuous mixing with a glass rod. After the temperature of a1 in the container reaches the design standard, the heating is stopped;

A3. Weigh ammonium bicarbonate and carboxymethyl chitosan according to the formula. After the temperature of a1 is cooled to normal temperature, add the remaining water, ammonium bicarbonate and carboxymethyl chitosan into the container, and stir and mix again. The preservative can be prepared after ammonium bicarbonate and carboxymethyl chitosan are completely dissolved in the stock solution a1 and filtered through a filter screen.

As preferably, the preparation method of described ascorbic acid extract comprises the following steps:

B1. Peel the lemons, and put the skins into the debittering solution for debittering treatment to obtain the skin extracts, crush the lemon pulp with a juicer, and squeeze out the lemon juice in the pulp through filter paper, Mix the leather extract with lemon juice to obtain a stock solution;

B2. The vitamin extract is added to the original solution, and the ascorbic acid extract can be obtained through stirring, filtration and separation processing; the present invention uses the ascorbic acid extract extracted from lemon, which has the functions of protein synthesis and immune function maintenance.

As preferably, the preparation method of described melatonin comprises the steps:

C1. Take the plant stem diameter for cleaning, pulverize the cleaned stem diameter, then pour the crushed stem diameter into a centrifuge and add an appropriate amount of hot water, and let it stand after centrifugal processing by the centrifuge;

C2, take out the supernatant liquid sent by centrifuge and filter, add propylene glycol to the supernatant liquid after filtering and carry out extraction process, obtain extract;

C3, pour the extract into a beaker and heat it, so that the propylene glycol in the extract is evaporated to obtain melatonin crystals, and then the melatonin crystals are dried and ground to obtain the melatonin; the present invention obtains the melatonin by As an antioxidant, melatonin can directly scavenge reactive oxygen species, activate antioxidant enzymes, and delay the aging of fruits and vegetables by scavenging free radicals, inhibiting ethylene synthesis, inducing protein oxidative damage repair enzymes, and regulating energy metabolism, thereby improving fruits and vegetables. Tolerance to biotic stress.

Preferably, the time for soaking in the antistaling agent of S. rhododendron in the S3 is 30-35 minutes.

Preferably, the concentration of nitrogen in the S4 fresh-keeping bag is 75%, the concentration of oxygen is 5%, and the concentration of carbon dioxide is 10%.

Preferably, the storage temperature in the S4 is 1-3°C.

Preferably, the concentration of the natamycin is 3.00ml/ml, and the concentration of the ascorbic acid extract is 7.00ml/ml.

Preferably, the temperature for heating a1 in the A2 is 30-35°C.

Embodiment 1, a kind of fresh-keeping method of utilizing melatonin to Bamboo radish, each component counted by its weight is: 10 parts of water, 8 parts of melatonin, 3 parts of natamycin, 5 parts of ascorbic acid extract parts, 1 part of ammonium bicarbonate and 2 parts of carboxymethyl chitosan.

The preparation method of the fresh agent comprises the following steps:

A1. Take a container of corresponding capacity according to the formula and carry out disinfection and sterilization treatment, then weigh 10 parts of water, and pour one half of the weighed water into the container, then weigh 5 parts of ascorbic acid extract according to the formula, and put the ascorbic acid The extract is poured into the container, and the water and the ascorbic acid extract are mixed with a glass rod to obtain the stock solution a;

A2. Weigh 8 parts of melatonin and 3 parts of natamycin according to the formula. After the water in the container and the ascorbic acid extract are completely fused, add melatonin and natamycin to the interior, and then add the remaining water in A1. One-half of the liquid is added to the inside of the container, and the container is heated. During the heating process, the original solution a1 is obtained by continuous mixing with a glass rod, and the heating is stopped after the temperature of a1 in the container reaches 30 °C;

A3. Weigh 1 part of ammonium bicarbonate and 2 parts of carboxymethyl chitosan according to the formula. After the temperature of a1 is cooled to normal temperature, add the remaining water, ammonium bicarbonate and carboxymethyl chitosan into the container, and then add it again. Stir and mix, and after the ammonium bicarbonate and carboxymethyl chitosan are completely dissolved in the stock solution a1, the preservative can be obtained by filtering through a filter screen;

A fresh-keeping method for Pseudomonas erythraea using melatonin, comprising the following steps:

S1, remove the root, remove the mud, and cut off the outer epidermis of the fungus cap from the freshly plucked Radix Rhododendron;

S2, put the Bamboo fungus rhododendron treated in S1 into sterile water for immersion for 10S, take out after soaking and quickly remove the surface water of Bamboo fungus orientalis by fan;

S3, put into the aseptic pool with preservatives and soak for 30 minutes after the treatment in S2, and seal the aseptic pool with plastic wrap, until reaching the soaking time, take out the bamboo fungus of the red leaf;

S4, put the fished out Radix Rhododendron into a fresh-keeping bag with a nitrogen concentration of 75%, an oxygen concentration of 5%, and a carbon dioxide concentration of 10%, and seal it, and store it in a dark and constant temperature environment, And the storage temperature is 1 ℃.

Embodiment 2, a kind of preservation method of utilizing melatonin to Bamboo fungus rhododendron, each component is counted according to its weight part as: 12 parts of water, 10 parts of melatonin, 5 parts of natamycin, 8 parts of ascorbic acid extract parts, 2 parts of ammonium bicarbonate and 3 parts of carboxymethyl chitosan.

The preparation method of the fresh agent comprises the following steps:

A1. Take a container of corresponding capacity according to the formula and carry out disinfection and sterilization treatment, then weigh 10 parts of water, and pour one half of the weighed water into the container, then weigh 8 parts of ascorbic acid extract according to the formula, and put the ascorbic acid The extract is poured into the container, and the water and the ascorbic acid extract are mixed with a glass rod to obtain the stock solution a;

A2. Weigh 10 parts of melatonin and 5 parts of natamycin according to the formula. After the water in the container and the ascorbic acid extract are completely fused, add melatonin and natamycin to the inside of the container, and then add the remaining water in A1. One-half of the liquid is added into the container, and the container is heated. During the heating process, the glass rod is continuously mixed to obtain the original solution a1, and the heating is stopped after the temperature of a1 in the container reaches 35 °C;

A3. Weigh 2 parts of ammonium bicarbonate and 3 parts of carboxymethyl chitosan according to the formula. After the temperature of a1 is cooled to normal temperature, add the remaining water, ammonium bicarbonate and carboxymethyl chitosan into the container, and then add it again. Stir and mix, and after the ammonium bicarbonate and carboxymethyl chitosan are completely dissolved in the stock solution a1, the preservative can be obtained by filtering through a filter screen;

A fresh-keeping method for Pseudomonas erythraea using melatonin, comprising the following steps:

S1, remove the root, remove the mud, and cut off the outer epidermis of the fungus cap from the freshly plucked Radix Rhododendron;

S2, put the Bamboo fungus rhododendron treated in S1 into sterile water for immersion for 15S, take out after soaking and quickly remove the surface moisture of Bamboo fungus orientalis by blower;

S3, put into the aseptic pool with preservatives and soak for 35 minutes after the treatment in S2, and seal the aseptic pool with plastic wrap, until reaching the soaking time, take out the bamboo fungus of the red leaf;

S4, put the fished out Radix Rhododendron into a fresh-keeping bag with a nitrogen concentration of 75%, an oxygen concentration of 5%, and a carbon dioxide concentration of 10%, and seal it, and store it in a dark and constant temperature environment, And the storage temperature is 3 ℃.

Performance Testing

In embodiment 1 to 2, utilize melatonin to the verification experiment of the preservation method of Bamboo fungus rhododendron

1. Test grouping

Test Group 1-2: Preservatives prepared in Examples 1 to 2

Control group: traditional nitrogen-filled sealing with low temperature and chemical agents.

2. Test method

Select the fresh Radix Radix et Rhizoma that is picked at the same time, divide into three groups in equal amounts after its cleaning, and be divided into experimental group 1, experimental group 2 and control group, carry out preservation treatment to experimental group 1 by the mode in embodiment 1, pass The mode in the embodiment 2 is carried out to the experimental group 2 to carry out the fresh-keeping treatment, the control group is carried out the fresh-keeping treatment by the mode of nitrogen-filled sealed low temperature and chemical agent, every day regularly to the external appearance, decay rate, respiration intensity, C. Dialdehyde content, browning index, energy level, superoxide anion production rate, H ₂ O ₂ content and antioxidant enzymes were observed and measured.

3. Test results

The test results are shown in the following table:

The test results of the herbal lavender repair lotion and the control group in Examples 1 to 2 below are shown below.

From the test results data in the above table, it can be seen that the effect of the preservative in the experimental group is far better than that of the traditional nitrogen-filled sealing at low temperature and the preservation effect of chemical agents.

Further, in the above-mentioned test, the external appearance, decay rate, respiration intensity, malondialdehyde content, browning index, energy level, superoxide anion of P. The performance of the speed, H ₂ O ₂ content and antioxidant enzymes was very good. The appearance, decay rate, respiration intensity, malondialdehyde content, browning index, energy level of P. , the production rate of superoxide anion, the content of H ₂ O ₂ and the performance of antioxidant enzymes were all lower than those of experimental group 1 and experimental group 2.

In the Present Invention Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, Modifications, substitutions and alterations, the scope of the invention is defined by the appended claims and their equivalents.

Claims (10)

1. a fresh-keeping method utilizing melatonin to Rhododendron chinensis, is characterized in that, comprises the following steps: S1, remove the root, remove the mud, and cut off the outer epidermis of the fungus cap from the freshly plucked Radix Rhododendron; S2, put the bamboo fungus rhododendron treated in S1 into sterile water for soaking, take out after soaking and quickly remove the surface moisture of the bamboo fungus rhododendron rhododendron through the blower; S3, put into the aseptic pool with antistaling agent and soak the treated in S2, and seal the aseptic pool with plastic wrap, until reaching the soaking time, take out the bamboo fungus of the red leaf; S4. Put the fished out rhododendron into a fresh-keeping bag and seal it, and store it in a dark and constant temperature environment. 2. a kind of fresh-keeping method utilizing melatonin according to claim 1, is characterized in that: in described S2, the time that the bamboo fungus rhododendron is soaked in sterile water is 10S-15S. 3. a kind of fresh-keeping method utilizing melatonin according to claim 1 is characterized in that: the preservative in described S3 comprises the raw material of following weight portion: 10-12 parts of water, 8-10 parts of melatonin, 3-5 parts of natamycin, 5-8 parts of ascorbic acid extract, 1-2 parts of ammonium bicarbonate and 2-3 parts of carboxymethyl chitosan; The preparation method of the fresh-keeping liquid comprises the following steps: A1. Take a container of corresponding capacity according to the formula and carry out disinfection and sterilization treatment, then take water, and pour one-half of the weighed water into the container, then weigh the ascorbic acid extract according to the formula, and pour the ascorbic acid extract into the container. In, water and ascorbic acid extract are mixed by glass rod to obtain stock solution a; A2. Weigh out the corresponding doses of melatonin and natamycin according to the formula. After the water in the container and the ascorbic acid extract are completely fused, add melatonin and natamycin to the inside of the container, and then add the remaining water in A1. One half is added into the container, and the container is heated. During the heating process, the raw liquid a1 is obtained by continuous mixing with a glass rod. After the temperature of a1 in the container reaches the design standard, the heating is stopped; A3. Weigh ammonium bicarbonate and carboxymethyl chitosan according to the formula. After the temperature of a1 is cooled to normal temperature, add the remaining water, ammonium bicarbonate and carboxymethyl chitosan into the container, and stir and mix again. The preservative can be prepared after ammonium bicarbonate and carboxymethyl chitosan are completely dissolved in the stock solution a1 and filtered through a filter screen. 4. a kind of preservation method utilizing melatonin according to claim 3 is characterized in that: the preparation method of described ascorbic acid extract comprises the following steps: B1. Peel the lemons, and put the skins into the debittering solution for debittering treatment to obtain the skin extracts, crush the lemon pulp with a juicer, and squeeze out the lemon juice in the pulp through filter paper, Mix the leather extract with lemon juice to obtain a stock solution; B2. The vitamin extract is added to the stock solution, and the ascorbic acid extract can be obtained through stirring, filtration and separation processing. 5. a kind of preservation method utilizing melatonin according to claim 3 is characterized in that: the preparation method of described melatonin comprises the steps: C1. Take the plant stem diameter for cleaning, pulverize the cleaned stem diameter, then pour the crushed stem diameter into a centrifuge and add an appropriate amount of hot water, and let it stand after centrifugal processing by the centrifuge; C2, take out the supernatant liquid sent by centrifuge and filter, add propylene glycol to the supernatant liquid after filtering and carry out extraction process, obtain extract; C3. Pour the extract into a beaker for heating, so that the propylene glycol in the extract is evaporated to obtain melatonin crystals, and then the melatonin crystals are dried and ground to obtain the melatonin. 6. a kind of fresh-keeping method utilizing melatonin according to claim 1, is characterized in that: in described S3, the time for soaking in preservative is 30-35 minutes. 7. a kind of fresh-keeping method utilizing melatonin according to claim 1 to Bamboo fungus rhododendron is characterized in that: in the described S4 fresh-keeping bag, the concentration of nitrogen is 75%, the concentration of oxygen is 5%, the concentration of carbon dioxide is 5%. The concentration is 10%. 8. a kind of fresh-keeping method of utilizing melatonin to bamboo fungus rhododendron according to claim 1, is characterized in that: the storage temperature in described S4 is 1-3 ℃. 9. a kind of fresh-keeping method utilizing melatonin according to claim 3 is characterized in that: the concentration of described natamycin is 3.00ml/ml, and the concentration of described ascorbic acid extract is 7.00ml/ml. 10 . The fresh-keeping method for Pseudomonas serrata using melatonin according to claim 3 , wherein the temperature for heating a1 in the A2 is 30-35° C. 11 .