CN115104688A - A kind of liquid preparation of non-denatured type II collagen and preparation method thereof - Google Patents

Abstract

The invention discloses a liquid preparation of non-denatured type II collagen and a preparation method thereof, belonging to the technical field of food processing. The liquid preparation is a colloidal suspension or a suspension type solid beverage which can be prepared into the colloidal suspension by adopting water with the temperature of below 35 ℃; the colloidal suspension is uniform and transparent, the suspension stability is kept for at least 30 days, and the colloidal suspension can be kept stable under the condition of pH 3-7. The non-denatured type II collagen-containing suspension type solid beverage prepared by the invention can be prepared by cold water, avoids the denaturation of non-denatured type II collagen caused by high temperature, keeps the functional activity of the non-denatured type II collagen to the maximum extent, increases the application range of the non-denatured type II collagen in the field of foods, and fills the gap that tablets or capsules can be only produced aiming at non-denatured type II collagen products in the field of health foods without other types of products.

Description

A kind of liquid preparation of non-denatured type II collagen and preparation method thereof

technical field

The invention belongs to the technical field of food processing, and in particular relates to a liquid preparation of non-denatured type II collagen and a preparation method thereof.

Background technique

Type II collagen widely exists in animal cartilage tissue. Non-denatured type II collagen refers to type II collagen that can still retain the triple helix structure of macromolecular collagen after extraction. The structure is a triple helix consisting of three peptide chains, the amino acids in each chain are held together by peptide bonds, and the three peptide chains are held in the triple helix by hydrogen bonds, dipole-dipole bonds, ionic bonds, and van der Waals interactions. in the structure. However, the triple helix structure of non-denatured type II collagen is easily affected by internal or external factors. For example, if the ambient temperature is higher than 35°C, the hydrogen bonds between the three peptide chains of non-denatured type II collagen can be continuously weakened and the peptide chains The helicity of the denatured collagen decreases, gradually straightens and unwinds, the disorder of the entire protein structure increases, and the biological activity of denatured type II collagen is significantly reduced.

Nondenatured type II collagen is an integral collagen component of articular cartilage and plays a crucial role in the development and maturation of chondrocytes. At the same time, clinical trials have been reported in the pathological research of rheumatoid arthritis and osteoarthritis and oral treatment of rheumatoid arthritis, and it has great potential for development and application in the prevention and treatment of rheumatoid arthritis and osteoarthritis.

Although non-denatured type II collagen has high physiological activity, due to the specific dense fibrous structure of non-denatured type II collagen, it is insoluble in water and the denaturation temperature is low, which limits the use of non-denatured type II collagen in the field of food and health food. Applications.

In recent years, the research on non-denatured type II collagen has gradually increased, but most of them focus on the research of extraction process, and the application of non-denatured type II collagen which is insoluble in water is less. In the prior art, chewable or tablet-type products containing type II collagen are unfavorable for the elderly to take, and cannot be subsequently applied to cold-chain foods such as live bacteria-type yogurt, fruit juice, jelly, etc. Increase the functionality of foods to improve knee health.

Therefore, it is urgent to provide a colloidal suspension of non-denatured type II collagen, and a solid granular beverage that can be brewed with low temperature water and maintain the activity of type II collagen, filling the field of health food for non-denatured type II collagen products that can only be produced Tablets or capsules without blanks for other types of products.

SUMMARY OF THE INVENTION

In order to solve the above problems, the present invention proposes a liquid preparation of non-denatured type II collagen, which is a colloidal suspension or a suspension-type solid beverage that can be prepared into a colloidal suspension with water below 35°C.

The colloidal suspension is uniform and transparent, and the suspension stability can be maintained for at least 30 days, and can be kept stable under the condition of pH 3-7.

The colloidal suspension is composed of the following raw materials: 2-10 parts of non-denatured type II collagen powder, 1-5 parts of hydrophilic colloid, 1-3 parts of oligosaccharides, 1-5 parts of hydrolyzed type II collagen, the balance Make up to 100 servings with water.

The suspension-type solid beverage is composed of the following raw materials: 25-70 parts of the colloidal suspension of claim 2 to remove moisture; 10-20 parts of fish collagen peptides, 5-10 parts of sea buckthorn powder, and 0-10 parts of citric acid , 0-5 parts of vitamin C, 0-3 parts of sodium citrate, 0.1-1 part of turmeric, 0-1 part of essence.

The suspended solid beverage is composed of the following raw materials: 5-30 parts of hydrophilic colloids, 10-15 parts of non-denatured type II collagen powder, 0-15 parts of oligosaccharides, 10-20 parts of fish collagen peptides, and hydrolyzed II 10-20 parts of collagen type, 5-10 parts of sea buckthorn powder, 0-10 parts of citric acid, 0-5 parts of vitamin C, 0-3 parts of sodium citrate, 0.1-1 part of turmeric, 0-1 part of essence.

Described hydrophilic colloid is one or more in xanthan gum, carrageenan, gellan gum, konjac gum, pectin, sodium alginate or sodium carboxymethyl cellulose;

The oligosaccharide is one or more of inulin, fructo-oligosaccharide, malt-oligosaccharide and xylo-oligosaccharide.

A preparation method of colloidal suspension, comprising the following steps:

a. Ingredients: add non-denatured type II collagen powder, oligosaccharides, and hydrolyzed type II collagen in sterile water according to the proportions, shear and disperse at 3000-5000rpm for 10-60min to obtain a coarse dispersion;

b. High pressure homogenization: the coarse dispersion liquid enters the high pressure homogenizer, and circulates 2-5 times under the homogenization pressure of 20MPa-60MPa to obtain the dispersion liquid, and the temperature is controlled at 18-28℃;

c. Sol: Add the hydrophilic colloid to hot water above 80°C at a material-to-liquid ratio of 1:50-1:100, shear at 3000-8000rpm for 5-30min to fully swell, and cool to 30-40 ℃ to obtain a colloidal solution;

d. Mixing: mixing the dispersion liquid in step b and the colloidal solution in step c uniformly to obtain a mixed liquid;

e. High-pressure micro-jet homogenization: the mixed liquid enters the high-pressure micro-jet homogenizer, and is homogenized 1-3 times under the condition of 80-150MPa, and the temperature is controlled at 28-38°C to obtain a suspension;

f. Cooling and standing: the suspension is allowed to stand and cool to obtain a gel;

g. Shear gel: The gel is sheared and dispersed at 1000-5000 rpm to obtain a colloidal suspension.

A preparation method of a suspended solid beverage, comprising the following steps:

1) The colloidal suspension described in claim 1 is subjected to freeze-drying treatment, the pre-freezing temperature is -80°C to -20°C, the drying time is 24-72h, the pulverizing time is 5-15min, and the impact speed is ^10-20 times s- ^1. The pulverization temperature is controlled at 5-18°C, to obtain a colloidal suspension with an average particle size of less than 40 μm to remove moisture, that is, the lyophilized powder of the colloidal suspension of non-denatured type II collagen;

2) Weigh step 1) colloidal suspension freeze-dried powder and raw materials according to the proportion, and mix them in a step-by-step gradient from light to heavy according to the weight of the raw materials to make the mixing degree more uniform; Carry out sub-packaging to obtain a suspended solid beverage.

A preparation method of a suspended solid beverage, comprising the following steps:

1) Weigh raw fish collagen peptide, sea buckthorn powder, citric acid, vitamin C, sodium citrate, turmeric and essence by proportioning, add in the colloidal suspension according to claim 1 after fully mixing, stir to obtain mixed liquid;

2) Freeze-dry the mixed solution in step 1), the pre-freezing temperature is -60°C, the drying time is 24-72h, the pulverization time is 5-15min, the impact speed is 10-20 times·s ^-1 , and the pulverization temperature is controlled at 5-18 ℃, pulverized and subpackaged with a composite film in a sterile environment to obtain a suspension-type solid beverage.

A preparation method of a suspended solid beverage, comprising the following steps:

1) The non-denatured type II collagen powder and the hydrophilic colloid are mixed according to the mass ratio of 1:9-9:1 and pulverized. Non-denatured type II collagen/hydrocolloid colloid composite powder with an average particle size of less than 40 μm;

2) Weigh the non-denatured type II collagen/hydrocolloid colloid composite powder and the remaining raw materials according to the ratio, mix them in a step-by-step gradient according to the weight of the raw materials, and mix them thoroughly. Carry out sub-packaging to obtain a suspended solid beverage.

The beneficial effects of the present invention are:

1. The colloidal suspension containing non-denatured type II collagen prepared by the present invention is uniform and transparent, and has good suspension stability; the suspension-type solid beverage containing non-denatured type II collagen can be prepared with cold water below 35° C. The thermal denaturation of type II collagen caused by higher than 35°C has good instant solubility, suspension, refreshing taste, rich nutrition, excellent health care ingredients, and is beneficial to bone and joint health.

2. The colloidal suspension containing the non-denatured type II collagen prepared by the present invention can stably suspend the water-insoluble non-denatured type II collagen, uniform and transparent, and improves the suspension of the non-denatured type II collagen.

3. The non-denatured type II collagen-containing suspension-type solid beverage prepared by the present invention has good flavor, instant solubility, good suspension stability, and has certain health-care ingredients, which is beneficial to bone and joint health.

4. The non-denatured type II collagen-containing suspended solid beverage prepared by the present invention can be prepared with cold water, which avoids the denaturation of non-denatured type II collagen caused by high temperature, maintains its functional activity to the greatest extent, and increases non-denatured type II collagen. The application range of protein in the food field fills the gap in the health food field that only tablets or capsules can be produced for non-denatured type II collagen products and no other types of products, and broadens the application range of non-denatured type II collagen.

Description of drawings

Fig. 1 is the preparation situation of the suspension-type solid beverage containing non-denatured type II collagen prepared in Example 2;

Fig. 2 is the preparation situation of the suspension type solid beverage containing non-denatured type II collagen prepared in Example 3;

Fig. 3 is the preparation situation of the suspension-type solid beverage containing non-denatured type II collagen prepared in Example 4;

Figures 4-a, 4-b, 4-c and 4-d respectively show the variation law of the backscattered light intensity with time of the non-denatured type II collagen-containing colloidal suspension and the control group measured by Turbiscan Lab in Example 5;

Fig. 5 is the change curve of TSI value with time of non-denatured type II collagen colloid suspension and control group in Example 5;

Fig. 6 is the particle size distribution of non-denatured type II collagen colloid suspension and control group in Example 5;

Figure 7 shows the state of the colloidal suspension containing non-denatured type II collagen in Example 5 before storage for 30 days

8 shows the state of the non-denatured type II collagen-containing colloid suspension in Example 5 after storage for 30 days.

Detailed ways

The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments:

Example 1

A kind of colloidal suspension of non-denatured type II collagen and preparation method thereof

Raw materials and proportions: 2-10 parts of non-denatured type II collagen powder, 1-5 parts of hydrophilic colloid, 1-3 parts of oligosaccharides, 1-5 parts of hydrolyzed type II collagen, and the rest to 100 parts with water .

A colloidal suspension of native type II collagen was prepared as follows:

a. Ingredients: add 30g of non-denatured type II collagen powder, 15g of inulin and 16g of hydrolyzed type II collagen into 1000mL sterile water, shear and disperse at 3000rpm for 15min to obtain a coarse dispersion.

b. High-pressure homogenization: the coarse dispersion liquid enters the high-pressure homogenizer, and is circulated twice under the homogenization pressure of 35MPa to obtain the dispersion liquid, and the temperature is controlled at 28°C;

c. Sol: add 10g xanthan gum to 1000mL sterile water: gellan gum: konjac gum=4:3:3 mixed colloid, add hot water at 80°C in a sterile environment, shear and disperse at 5000rpm for 10min , make it fully swollen, and cooled to 35 °C to obtain a colloidal solution;

d. Mixing: uniformly mix the dispersion obtained in step b and the colloidal solution obtained in step c at 35°C to obtain a mixed solution;

e. High-pressure micro-jet homogenization: homogenize the mixed solution in a high-pressure micro-jet homogenization machine for 2 times under the condition of 100MPa, and control the temperature at 30°C to obtain a suspension;

f. Cooling and standing: standing the mixed dispersion and cooling to obtain a hydrogel with a certain gel strength;

g. Shear gel: The gel is sheared and dispersed at 2000 rpm to obtain a colloidal suspension.

Example 2

A kind of suspension type solid beverage containing non-denatured type II collagen and preparation method thereof

Weigh 25 parts of lyophilized powder of colloidal suspension containing non-denatured type II collagen, 15 parts of inulin, 15 parts of fish collagen peptide, 20 parts of hydrolyzed type II collagen, 10 parts of sea buckthorn powder, 5 parts of citric acid, vitamins 2 parts of C, 2 parts of sodium citrate, 1 part of turmeric, 5 parts of essence.

The preparation method includes the preparation of lyophilized powder of colloidal suspension containing non-denatured type II collagen and the preparation of solid beverage. The preparation method is as follows:

1) Preparation of lyophilized powder of colloidal suspension containing non-denatured type II collagen

The colloidal suspension of non-denatured type II collagen was prepared in the same manner as in Example 1, and the colloidal suspension was pre-frozen in a -80° C. refrigerator for 3 hours, and dried by freeze drying equipment for 48 hours.

2) Preparation of solid beverages

Weigh the lyophilized powder of colloidal suspension containing non-denatured type II collagen and other required raw materials according to the ratio, mix the raw materials with close weight ratios first, and start from the lighter weight raw materials for step-by-step gradient mixing, and the mixing order is in turn For: turmeric, vitamin C, sodium citrate, citric acid, essence, sea buckthorn powder, inulin, fish collagen peptides, hydrolyzed type II collagen, colloidal suspension lyophilized powder containing non-denatured type II collagen; well mixed After homogenization, the composite film is used for sub-packaging under aseptic conditions to obtain a solid beverage containing non-denatured type II collagen with a specification of 5g/bag.

How to eat: Mix with 100mL of 25℃ water. The photos of the change of the mixing situation of the non-denatured type II collagen-containing suspension solid beverage at 0s, 10s, 20s and 30s are shown in Figure 1, and the colloid can be obtained within 30s after fully stirring. Suspension for drinking.

Example 3

A kind of suspension type solid beverage containing non-denatured type II collagen and preparation method thereof

Raw materials and proportions: 8 parts of non-denatured type II collagen, 12 parts of xanthan gum, 30 parts of inulin, 20 parts of fish collagen peptide, 20 parts of hydrolyzed type II collagen, 5 parts of sea buckthorn powder, and 5 parts of curcumin.

The preparation method includes the preparation of a colloidal suspension containing non-denatured type II collagen and the preparation of a solid beverage. The preparation method is as follows:

1) Preparation of colloidal suspension containing native type II collagen

Same as Example 1.

2) Preparation of solid beverages

Weigh other required raw materials other than non-denatured type II collagen powder according to the proportion, mix well and add them to the colloidal suspension containing non-denatured type II collagen, stir evenly, freeze-dry the mixture and pre-freeze it The temperature is -60°C, the drying time is 48h, and then pulverized after drying. The pulverization time is 10min, the impact speed is 20 times·s ^-1 , the pulverization temperature is 18°C, and it is passed through a 200-mesh sieve. A solid beverage containing non-denatured type II collagen was obtained.

Edible method: Use 100mL of 30 ℃ water to mix, and the photos of the change of the mixing situation of the non-denatured type II collagen suspension solid beverage at 0s, 10s, 20s and 30s are shown in Figure 2, and the colloid can be obtained within 30s of thorough stirring. Suspension for drinking.

Example 4

A kind of suspension type solid beverage containing non-denatured type II collagen and preparation method thereof

Raw materials and proportions: 8 parts of non-denatured type II collagen, 12 parts of xanthan gum, 30 parts of inulin, 20 parts of fish collagen peptide, 20 parts of hydrolyzed type II collagen, 5 parts of sea buckthorn powder, and 5 parts of curcumin.

The preparation method includes the preparation of non-denatured type II collagen/hydrocolloid colloid composite powder and the preparation of solid beverages, and the details are as follows:

1) Preparation method of non-denatured type II collagen/hydrocolloid colloid composite powder

Weigh non-denatured type II collagen and xanthan gum in a ratio of 2:3, mix them evenly, and pour into a high-energy nano-impact mill for grinding. The grinding time is 6h, the cooling temperature is 2°C, the machine power is 4kw, the speed is 400r/min, and the grinding medium Zirconia ceramic balls are ground to obtain non-denatured type II collagen/hydrocolloid colloid composite powder.

2) Preparation of solid beverages

Weigh the required raw materials according to the proportion, and proceed step by step in the order of sea buckthorn powder, curcumin, fish collagen peptide, hydrolyzed type II collagen, inulin, non-denatured type II collagen/hydrocolloid colloid composite powder Gradient mixing, after fully mixing and evenly mixing, under aseptic conditions, the composite membrane is used for sub-packaging to obtain a non-denatured type II collagen-containing solid beverage with a specification of 5g/bag.

Edible method: use 100mL of 10 ℃ water to mix, and the photos of the change of the mixing situation of the non-denatured type II collagen-containing suspension solid beverage at 0s, 10s, 20s and 30s are shown in Figure 3, and the colloid can be obtained within 30s of full stirring. Suspension for drinking.

Example 5

Study on the Stability and Storage of Colloidal Suspension of Non-denatured Type II Collagen

Taking the product obtained in Example 1 as the test sample, and setting three control groups at the same time, as shown in Table 1, the stability was tested by the Turbiscan Lab stability analyzer, and the transmission of the four samples was measured at 25 ° C respectively. Light and backscattered light intensity versus time. First, set the required test temperature, put the sample to be tested in the sample cell, the sample height is 0-42mm, equilibrate at this temperature for 30min, and then use the continuous scanning method to collect a set of data every 25s, continuously After scanning for 30 min, the curve of the intensity of transmitted light and backscattered light with time can be obtained. The transmitted light signal is almost 0 in the test result. Therefore, the variation law of backscattered light intensity with time is analyzed as shown in Figure 4-a to Figure 4-d, and the variation curve of TSI value with time within the specified measurement time is calculated and drawn as shown in Figure 4-d. 5 shown.

It can be seen from the figure that there is a significant difference in the stability of the samples in the experimental group and the control group. The change trend of the three ΔBS values in the control group is similar with the passage of time, that is, precipitation at the bottom, large fluctuation in the middle, and clarification at the top, it can be seen that the stability of the samples in the control group is not good. In comparison, the untreated non-denatured type II collagen had the worst stability, and the ΔBS image showed a more obvious bulge at the bottom, indicating more sedimentation, and a clear concave peak at the top, indicating that the top was clear, and again It is proved that the untreated non-denatured type II collagen has extremely poor solubility in water, which is not conducive to use as a liquid preparation (as shown in Figure 4-a). Even if it is made into a suspension, its dispersibility cannot be changed well (as shown in Figure 4-b), and precipitation and stratification will still occur; for the non-denatured type II collagen treated by the above method, a convex peak occurs. Shifting to the right (as shown in Figure 4-c) indicates that the dispersibility of the sample is enhanced and the sedimentation is reduced. This method is effective in improving the suspension of non-denatured type II collagen, but the upper layer will still be clarified. The data of the experimental group showed that there was no obvious bulge at the bottom of the ΔBS image of the treated non-denatured type II collagen colloid suspension, no significant change in ΔBS in the middle, no obvious delamination at the top, and good uniformity and stability (as shown in Figure 4). -d). Finally, in order to characterize the overall stability of the sample, the relative stability of each system can be compared with the turbiscan stability index (TSI) defined by Turbiscan Easysoft software. The results are shown in Figure 5; the smaller the TSI value, the more stable the system is. , TSI results are consistent with the above analysis, indicating the uniqueness and effectiveness of this method.

Table 1 Setting method of experimental group and control group

Similarly, taking the product obtained in Example 1 as the experimental group, as shown in Table 1, three samples were prepared as the control group, and the particle size distribution was measured by a static light scattering particle size analyzer. The results are shown in Figure 6. The results show that this method can The particle size of the system is significantly reduced, and the particle size distribution of the experimental group is in a “skinny” single-peak shape, which is more conducive to the dispersion stability of the system, while the control group has a larger particle size and a wider peak area, which is not conducive to the stability of the system. stable, consistent with the above results.

The samples of the experimental group were placed in citric acid solution with pH 3.8 and pH 5, and in Brix sucrose aqueous solution with a soluble solid content of 10°, respectively, and stored at 35° C. under dark conditions for 30 days. Samples were taken for inspection before and after storage. The test item is sensory analysis, and the test results are shown in Table 2.

Table 2 Stability test results of colloidal suspensions containing non-denatured type II collagen

Turbiscan Lab stability and particle size distribution test results show that the colloidal suspension has good stability and particle size distribution. Taking the state pictures of the non-denatured type II collagen colloidal suspension before and after storage (shown in Figure 7 and Figure 8) as a control, the stability test results show that the colloidal suspension can be in low acid, high acid and medium. It can be stored for 30 days in a sexual environment and below 35°C in the dark, and it still maintains good stability.

Claims (10)

1. A liquid preparation of non-denatured type II collagen, characterized in that the liquid preparation is a colloidal suspension or a suspension-type solid beverage that can be prepared into a colloidal suspension with water below 35°C. 2. the liquid preparation of a kind of non-denatured type II collagen according to claim 1, is characterized in that, described colloid suspension, uniform and transparent, suspension stability can keep at least 30 days, all can keep under pH3-7 condition Stablize. 3. the liquid preparation of a kind of non-denatured type II collagen according to claim 1, is characterized in that, described colloidal suspension is made up of following raw materials: 2-10 parts of non-denatured type II collagen powder, 1-5 parts of Hydrocolloids, 1-3 parts of oligosaccharides, 1-5 parts of hydrolyzed type II collagen, and the rest to 100 parts with water. 4. the liquid preparation of a kind of non-denatured type II collagen according to claim 1, is characterized in that, described suspension type solid beverage is made up of following raw material: the product 25-70 of the described colloidal suspension of claim 2 that removes moisture parts; 10-20 parts of fish collagen peptides, 5-10 parts of sea buckthorn powder, 0-10 parts of citric acid, 0-5 parts of vitamin C, 0-3 parts of sodium citrate, 0.1-1 parts of turmeric, 0-1 parts of essence share. 5. the liquid preparation of a kind of non-denatured type II collagen according to claim 1, is characterized in that, described suspension type solid beverage is made up of following raw materials: hydrophilic colloid 5-30 parts, non-denatured type II collagen powder 10-15 parts, 0-15 parts oligosaccharides, 10-20 parts fish collagen peptides, 10-20 parts hydrolyzed collagen type II, 5-10 parts sea buckthorn powder, 0-10 parts citric acid, vitamin C 0- 5 parts, 0-3 parts of sodium citrate, 0.1-1 part of turmeric, 0-1 part of essence. 6. according to the liquid preparation of a kind of non-denatured type II collagen of claim 3, 4 or 5, it is characterized in that, Described hydrophilic colloid is one or more in xanthan gum, carrageenan, gellan gum, konjac gum, pectin, sodium alginate or sodium carboxymethyl cellulose; The oligosaccharide is one or more of inulin, fructo-oligosaccharide, malt-oligosaccharide and xylo-oligosaccharide. 7. the preparation method of the described colloidal suspension of claim 3, is characterized in that, comprises the following steps: a. Ingredients: add non-denatured type II collagen powder, oligosaccharides, and hydrolyzed type II collagen in sterile water according to the proportions, shear and disperse at 3000-5000rpm for 10-60min to obtain a coarse dispersion; b. High pressure homogenization: the coarse dispersion liquid enters the high pressure homogenizer, and circulates 2-5 times under the homogenization pressure of 20MPa-60MPa to obtain the dispersion liquid, and the temperature is controlled at 18-28℃; c. Sol: Add the hydrophilic colloid to hot water above 80°C at a material-to-liquid ratio of 1:50-1:100, shear at 3000-8000rpm for 5-30min to fully swell, and cool to 30-40 ℃ to obtain a colloidal solution; d. Mixing: mixing the dispersion liquid in step b and the colloidal solution in step c uniformly to obtain a mixed liquid; e. High-pressure micro-jet homogenization: the mixed liquid enters the high-pressure micro-jet homogenizer, and is homogenized 1-3 times under the condition of 80-150MPa, and the temperature is controlled at 28-38°C to obtain a suspension; f. Cooling and standing: the suspension is allowed to stand and cool to obtain a gel; g. Shear gel: The gel is sheared and dispersed at 1000-5000 rpm to obtain a colloidal suspension. 8. the preparation method of the described suspension type solid beverage of claim 4, is characterized in that, comprises the following steps: 1) The colloidal suspension described in claim 1 is subjected to freeze-drying treatment, the pre-freezing temperature is -80°C to -20°C, the drying time is 24-72h, the pulverizing time is 5-15min, and the impact speed is ^10-20 times s- ^1. The pulverization temperature is controlled at 5-18°C, to obtain a colloidal suspension with an average particle size of less than 40 μm to remove moisture, that is, the lyophilized powder of the colloidal suspension of non-denatured type II collagen; 2) Weigh step 1) colloidal suspension freeze-dried powder and raw materials according to the proportion, mix them in a step-by-step gradient from light to heavy according to the weight of the raw materials, and after fully mixing, sub-pack with composite membranes in a sterile environment to obtain suspension type solid beverages. 9. the preparation method of the described suspension type solid beverage of claim 5, is characterized in that, comprises the following steps: 1) Weigh raw fish collagen peptide, sea buckthorn powder, citric acid, vitamin C, sodium citrate, turmeric and essence by proportioning, add in the colloidal suspension according to claim 1 after fully mixing, stir to obtain mixed liquid; 2) Freeze-dry the mixed solution in step 1), the pre-freezing temperature is -60°C, the drying time is 24-72h, the pulverization time is 5-15min, the impact speed is 10-20 times·s ^-1 , and the pulverization temperature is controlled at 5-18 ℃, pulverized and subpackaged with a composite film in a sterile environment to obtain a suspension-type solid beverage. 10. a preparation method of the described suspension type solid beverage of claim 5, is characterized in that, comprises the following steps: 1) The non-denatured type II collagen powder and the hydrophilic colloid are mixed according to the mass ratio of 1:9-9:1 and pulverized. Non-denatured type II collagen/hydrocolloid colloid composite powder with an average particle size of less than 40 μm; 2) Weigh the non-denatured type II collagen/hydrocolloid colloid composite powder and the remaining raw materials according to the ratio, mix them in a step-by-step gradient according to the weight of the raw materials, and mix them thoroughly. Carry out sub-packaging to obtain a suspended solid beverage.

Images