CN115096871B - Detection device applied to multichannel SERS micro-fluidic chip - Google Patents

Abstract

This application provides a detection device applied to a multi-channel SERS microfluidic chip, including a microfluidic pipeline and a detection chip; the detection chip includes multiple, and is connected in parallel to the output pipeline end of the microfluidic pipeline , the input pipe end of the microfluidic pipeline is used to input the sample solution to be detected; wherein, each detection chip includes a SERS substrate applied to detect different properties to be detected, and the SERS substrate includes the following nanoporous silver structure Either: a nanoporous silver structure modified by a single-layer lipid film on the surface, a nanoporous silver structure modified by a double-layer lipid film on the surface, and a nanoporous silver structure; the SERS substrate adsorbs the sample solution to be detected The molecules to be detected in the detection chip are detected by a handheld Raman spectrometer to detect the Raman signal of the molecules to be detected adsorbed on the detection chip. It solves the problem that it is difficult to simultaneously detect the substances to be detected with different properties and the detection efficiency is low, realizes multi-channel simultaneous detection, and improves the detection efficiency.

Description

Detection device applied to multi-channel SERS microfluidic chip

technical field

The present application relates to the technical field of detection devices, in particular to a detection device applied to a multi-channel SERS microfluidic chip.

Background technique

Surface-enhanced Raman scattering (SERS) technology is a new type of trace analysis technology. It can obtain information on the influence of genes, chemical bonds and microenvironment on the structure of samples from the molecular level, and obtain its fingerprints in real time, with a large amount of information. In addition to the characteristics of high sensitivity, strong specificity, no labeling, no damage, and real-time detection, it will not be interfered by media such as solutions, and is widely used in biochemical detection and other fields. Microfluidic chip is a core technology applied in the field of micro total analysis system.

At present, the common method of combining SERS technology with microfluidic chips has been improved from one-time detection to reusable. For example, application number 201810524022.6 discloses a reusable high-throughput SERS microfluidic chip and its application. It includes a reaction chip and a detection chip connected in series through a microfluidic pipeline; the reaction chip is used for mixed reaction of a liquid sample containing heavy metal ions and a DNase reagent; the detection chip is used for reacting the reaction chip Reactants are tested. Also provided is the application of the aforementioned reusable high-throughput SERS microfluidic chip, which is used to detect the content of trace harmful metal ions in the water environment. The SERS microfluidic chip designed by it can be used repeatedly, the process is simple, the cost is low, the efficiency is high, and it can be mass-produced. It can detect multiple data at one time, and has the characteristics of fast, efficient and high-throughput detection. Although it can be used repeatedly, it cannot be multi-channel and multi-type simultaneous detection for different analytes due to their different hydrophilic and lipophilic properties, low detection efficiency, and weak signal strength.

Contents of the invention

The main purpose of the present application is to provide a detection device applied to a multi-channel SERS microfluidic chip, aiming at solving the problems that it is difficult to simultaneously detect different properties of the substances to be detected and the detection efficiency is low.

In order to achieve the above purpose, the present application provides a detection device applied to a multi-channel SERS microfluidic chip, including a microfluidic pipeline and a detection chip;

The detection chip comprises a plurality, and is connected in parallel to the output pipeline end of the microfluidic pipeline, and the input pipeline end of the microfluidic pipeline is used to input the sample solution to be detected;

Wherein, each of the detection chips includes a SERS substrate applied to detect substances with different properties to be detected, and the SERS substrate includes any one of the following nanoporous silver structures: a nanoporous surface modified with a single layer of lipid film Silver structure, nanoporous silver structure modified by bilayer lipid membrane on the surface, nanoporous silver structure;

The SERS substrate adsorbs the molecules to be detected in the sample solution to be detected, and the Raman signal of the molecules to be detected adsorbed on the detection chip is detected by a handheld Raman spectrometer.

Further, the manufacturing process of the SERS substrate includes:

The silver flakes with a purity of 99.9% are washed with double deionized water, and the washed silver flakes are placed in potassium chloride water with a preset concentration for electrochemical reaction to obtain silver flakes plated with silver chloride;

Stirring the solution in which the silver chloride-plated silver flakes are located, placing the silver chloride-plated silver flakes in a reducing agent, and reducing them to nanoporous silver flakes by the reducing agent in situ;

The porous silver sheet was washed with deionized water and ethanol to obtain a nanoporous silver structure.

Further, potassium chloride water is electrochemically reacted with a voltage of 0.15 volts.

Further, the diameter of each pore in the nanoporous silver structure is 60-80 nm.

Further, the detection chip includes three, namely a first detection chip, a second detection chip and a third detection chip;

The SERS substrate in the first detection chip adopts a nanoporous silver structure modified with a monolayer lipid film on the surface;

The SERS substrate in the second detection chip adopts a nanoporous silver structure with a bilayer lipid film modification on the surface;

The SERS substrate in the third detection chip adopts the nanoporous silver structure.

Further, a monolayer lipid film is deposited on the nanoporous silver structure through the technique of constructing an ordered ultrathin molecular film, so as to obtain a nanoporous silver structure with a monolayer lipid film modified on the surface.

Further, the step of modifying the surface of the nanoporous silver structure with monolayer lipid membrane modification includes:

A certain amount of lipid solution is dropped on the water surface through a Hamilton micro-syringe, and the lipid solution is evaporated to form a lipid film;

Squeezing the lipid film against the lipid film at a preset speed until the lipid film bears a target surface pressure;

The glass slide in the water and the nanoporous silver structure on the glass slide are pulled up at a specified speed until the nanoporous silver structure is exposed to the water surface, and a monolayer of lipid on the surface can be obtained. Membrane-modified nanoporous silver structures.

Further, the step of modifying the surface of the nanoporous silver structure with monolayer lipid membrane modification includes:

A certain amount of lipid solution is dropped on the water surface through a Hamilton micro-syringe, and the lipid solution is evaporated to form a lipid film;

Squeezing the lipid film against the lipid film at a preset speed until the lipid film bears a target surface pressure;

Pulling up the glass slide in the water and the nanoporous silver structure on the glass slide at a specified speed until the nanoporous silver structure is exposed to the water surface;

The nanoporous silver structure is inserted into the water again, so as to realize the nanoporous silver structure with double-layer lipid film modification on the surface.

Further, the reducing agent is sodium borohydride solution.

Further, the input pipeline end of the microfluidic pipeline is set as a Y-shaped pipeline, the chamber where the sample solution to be detected is located communicates with the first port of the Y-shaped pipeline, and the second port of the Y-shaped pipeline The port communicates with the cavity where the cleaning solution for cleaning the microfluidic pipeline is located.

The detection device applied to the multi-channel SERS microfluidic chip provided in this application has the following beneficial effects:

By setting up multiple detection chips in parallel, and setting a nanoporous structure on the detection chip for detecting the molecules to be detected with corresponding properties, to solve the problem of difficult simultaneous detection and low detection efficiency of different properties to be detected, and realize multi-channel Simultaneous detection to improve detection efficiency.

Description of drawings

FIG. 1 is a schematic structural diagram of a detection device applied to a multi-channel SERS microfluidic chip according to an embodiment of the present application.

The realization, functional features and advantages of the present application will be further described in conjunction with the embodiments and with reference to the accompanying drawings.

detailed description

In order to make the purpose, technical solution and advantages of the present application clearer, the present application will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present application, and are not intended to limit the present application.

Referring to FIG. 1 , an embodiment of the present application provides a detection device applied to a multi-channel SERS microfluidic chip, including a microfluidic pipeline 2 and a detection chip 1; the detection chip 1 includes multiple, and parallel Connected to the output pipeline end of the microfluidic pipeline 2, the input pipeline end of the microfluidic pipeline 2 is used to input the sample solution 3 to be detected; wherein, each detection chip 1 includes a SERS for detecting substances to be detected with different properties The substrate, the SERS substrate includes any one of the following nanoporous silver structures 5: a nanoporous silver structure 51 with a single-layer lipid film modification on the surface, a nanoporous silver structure 52 with a double-layer lipid film modification on the surface, Pure nanoporous silver structure 53; the SERS substrate adsorbs the molecules to be detected in the sample solution to be detected, and the Raman signal of the molecules to be detected adsorbed on the detection chip 1 is detected by a handheld Raman spectrometer.

This application is applied to the detection of different properties of the samples to be detected in the liquid to be detected, more specifically by modifying the surface of the nanoporous silver structure 5 to achieve enrichment and detection of different properties of the samples to be detected, such as The samples to be tested with different properties include at least one of: hydrophilic pesticides, lipophilic pesticides, and charged pesticides; in order to realize multi-channel detection of the samples to be tested and improve detection efficiency.

In this embodiment, the microfluidic pipeline 2 is used to transmit the sample solution to be detected to the area to be detected, and the microfluidic pipeline 2 includes an input pipeline end and an output pipeline end, wherein the sample solution to be detected passes through the input pipeline end The incoming flow flows to the output pipeline end, where multiple detection chips 1 are connected in parallel at the output pipeline end, and each detection chip 1 includes a SERS substrate used to detect analytes with different properties. SERS refers to surface-enhanced Raman scattering technology , is a new type of trace analysis technology, which can obtain information on the influence of genes, chemical bonds and microenvironment on the sample structure from the molecular level, and obtain its fingerprints in real time, with a large amount of information. In addition to high sensitivity, strong specificity, No labeling, no damage, real-time detection, etc., and will not be interfered by media such as solutions; in this embodiment, the SERS substrate includes nanoporous silver structures 5, specifically nanoporous silver structures with different surface modifications 5. For example, a nanoporous silver structure 51 modified by a single-layer lipid film on the surface, or a nanoporous silver structure 52 modified by a double-layer lipid film on the surface, or a nanoporous silver structure 53; The porous silver structure 5 is enlarged and displayed to observe whether the pore shape distribution of the nanoporous silver structure 5 on the SERS substrate is uniformly distributed, or to observe whether the pore shape distribution of the nanoporous silver structure 5 modified by adding a lipid film is uniform; if In the chip where the nanoporous silver structure 52 modified by a double-layer lipid film on the surface is observed by a high-magnification scanning electron microscope, the morphology distribution of the nanoporous silver structure 52 modified by a double-layer lipid film on the surface is uneven, then Indicates that the nanoporous silver structure 52 modified by a double-layer lipid film on the surface has uneven pores, which is a defective product; if the nanoporous silver structure 51 modified by a single-layer lipid film on the surface is observed by a high-magnification scanning electron microscope In the chip where the morphology distribution of the nanoporous silver structure 51 modified by a single-layer lipid film on the surface is uniform, it means that the pore shape of the nanoporous silver structure 51 modified by a single-layer lipid film on the surface is uniform, which is a good product . The SERS substrate is used to adsorb the molecules to be detected in the sample solution to be detected, and the Raman signal of the molecules to be detected in the nanoporous silver structure 5 on the detection chip 1 is detected by a handheld Raman spectrometer. If the molecule is detected, the Raman signal will be amplified to the sixteenth power of the original Raman signal. The nanoporous silver structure 5 is set to amplify the Raman signal of the adsorbed chemical molecules, and then view the sample solution to be detected. Whether there are different substances to be detected that need to be detected.

As in a specific embodiment, the detection chip 1 includes two, the two detection chips 1 are connected in parallel at the end of the output pipeline, and one of the two detection chips 1 is a nanometer surface modified with a single layer of lipid film The porous silver structure 51 (that is, the detection chip is the first detection chip 11) is used for adsorption and detection of lipophilic pesticides; the other detection chip 1 is a nanoporous silver structure 52 with a double-layer lipid membrane modified on the surface (That is to say, the detection chip is the second detection chip 12), which is used to adsorb and detect charged pesticides; the sample solution to be detected is split from the output pipe end to the two detection chips 1, if the handheld Raman spectrometer Detect the Raman signal of the molecule to be detected in the nanoporous silver structure 5 located on the detection chip 1, when adsorbed to the molecule to be detected with corresponding properties, such as a nanoporous silver structure 51 modified by a single layer of lipid film on the surface If lipophilic pesticides are adsorbed to the first detection chip 11, the Raman signal will be amplified to the sixth power of ten times the original Raman signal, indicating that a nanoporous silver structure modified by a single-layer lipid film has occurred on the surface. 51 is adsorbed to the lipophilic pesticide, that is, there is lipophilic pesticide in the sample solution to be detected; if the Raman signal of the molecule to be detected located on the nanoporous silver structure 5 on the detection chip 1 is detected by a handheld Raman spectrometer, When the molecule to be detected is adsorbed to the corresponding property, such as the charged pesticide is adsorbed to the second detection chip 12 where the nanoporous silver structure 52 with a double-layer lipid membrane modified on the surface is located, the Raman signal will be amplified to the original The ten-sixth power signal of the Raman signal indicates that the nanoporous silver structure modified with a bilayer lipid membrane on the surface adsorbs charged pesticides, that is, there are charged pesticides in the sample solution to be detected. The molecules to be detected with different properties in the same sample solution to be detected are detected through multiple channels, so that the molecules to be detected with different properties can be detected simultaneously, and the detection efficiency is improved. In this embodiment, the magnification of the electron microscope is based on the fact that the morphology of the nanoporous structure can be observed, and there is no specific limitation on the magnification here. In other embodiments, multiple parallel detection chips 1 can also be set up for detecting different properties, and here, the number of parallel detection channels is not limited.

In this embodiment, the manufacturing process of the SERS substrate includes:

Step 1, wash the silver flakes with a purity of 99.9% by double deionized water, and place the washed silver flakes in potassium chloride water with a preset concentration for electrochemical reaction to obtain silver flakes plated with silver chloride;

Step 2, stirring the solution where the silver chloride-plated silver flakes are located, placing the silver chloride-plated silver flakes in a reducing agent, and reducing them to nanoporous silver flakes in situ by the reducing agent;

Step 3, washing the porous silver sheet with deionized water and ethanol to obtain nanoporous silver structure 53 .

In this example, in step 1, the silver flakes with a purity of 99.9% were washed with double deionized water (dd-H2O), and the washed silver flakes were placed in an aqueous electrolyte with a predetermined concentration of potassium chloride for electrolysis. Chemical reaction, wherein the concentration of potassium chloride aqueous solution can be preset as 1 mole per liter of potassium chloride aqueous solution, at this time, the surface of the metallic silver flakes changes from lustrous silver to dark yellow, until the metallic silver flakes become dark After the yellow color, it means that the electrochemical reaction stays overnight, and the silver flakes coated with silver chloride can be obtained; the aqueous solution of the silver flakes coated with silver chloride is stirred, so that the silver flakes are in the dynamically stirred liquid, and then the silver flakes are Placed in the reducing agent, the original taste of the reducing agent is reduced to nanoporous silver flakes. The silver flakes quickly enter the reducing agent from the dynamically stirred liquid. The silver sheet of silver chloride is reduced, and there are bubbles in the sodium borohydride solution at this time, and the bubbles in the sodium borohydride solution are eliminated, and then it is judged that the silver sheet of the silver chloride plating has been reduced to a nanoporous silver sheet, and then by removing Washing with deionized water and ethanol to obtain the desired nanoporous silver structure, in a specific embodiment, first wash the reduced nanoporous silver sheet with deionized water, then wash with ethanol, and the nanoporous silver The sheet was cross-washed three times with deionized water and three times with ethanol to obtain the desired nanoporous silver structure53.

The generation principle of the nanoporous silver structure 53 is that pure silver with a smooth surface is electrochemically oxidized to silver chloride, and the original reduction is induced by a reducing agent to produce a porous silver structure with a nanoporous structure.

In this embodiment, the reducing agent is a substance that loses electrons or has electron deviations in the oxidation-reduction reaction. The reducing agent itself is reducing and is oxidized, and its products are called oxidation products. The reduction and oxidation reactions proceed simultaneously, that is to say, the reducing agent itself is oxidized to become an oxide while undergoing an oxidation reaction with the reduced substance. A reactant in which the valency of a substance is increased is a reducing agent.

In this embodiment, potassium chloride water is electrochemically reacted with a voltage of 0.15 volts. Using 0.15V voltage to electrochemically react the solution of potassium chloride water, the desired nanoporous pore size can be obtained uniformly, and the pore size is 60-80nm, which is convenient for subsequent observation through an electron microscope.

In this embodiment, the diameter of each pore in the nanoporous silver structure 53 is 60-80 nm. The pore diameter of 60-80nm is used, so that each pore diameter in the nanoporous silver structure 53 is within this size range, so that the precise control of the nanoporous silver structure 53 is realized, which is convenient for subsequent surface modification and electron microscope observation.

In this embodiment, the detection chip 1 includes three, respectively the first detection chip 11, the second detection chip 12 and the third detection chip 13; Modified nanoporous silver structure 51; the SERS substrate in the second detection chip adopts a nanoporous silver structure 52 with a double-layer lipid film on the surface; the SERS substrate in the third detection chip adopts a silver sheet nanoporous silver structure 53 .

In this embodiment, three detection chips 1 for detecting different molecules to be detected in the sample solution to be detected are provided, namely the first detection chip 11, the second detection chip 12 and the third detection chip 13, wherein the first The SERS substrate of the detection chip 11 adopts a nanoporous silver structure 51 modified with a single-layer lipid film on the surface, which is used to adsorb lipophilic pesticides in the molecules to be detected. The Raman signal of the molecule to be detected in the nanoporous silver structure 51 modified with a single layer of lipid film on the surface is detected. When lipophilic pesticides are adsorbed to the first detection chip 5 where the silver structure 51 is located, the Raman signal will be amplified to ten times the sixth power of the original Raman signal. If the porous silver structure 51 is adsorbed to the lipophilic pesticide, it means that there is lipophilic pesticide in the sample solution to be detected; The Raman signal of the molecule to be detected in the porous silver structure 51 is detected. If no amplified signal is found in the Raman signal, that is, no Raman signal is detected or the Raman signal is very weak, it means that a single layer of lipid film modification occurs on the surface. The processed nanoporous silver structure 51 does not adsorb lipophilic pesticides, that is, there is no lipophilic pesticides in the sample solution to be detected or the concentration of lipophilic pesticides is too low to be calculated. The SERS substrate of the second detection chip 12 adopts a nanoporous silver structure 52 modified with a double-layer lipid film on the surface, which is used to adsorb whether there is a charged pesticide in the molecule to be detected; The Raman signal of the molecule to be detected in the nanoporous silver structure 52 that has been modified by a double-layer lipid film on the surface of the chip 12 is detected. If the charged pesticide is adsorbed to the second detection chip 12 where the processed nanoporous silver structure 52 is located, the Raman signal will be amplified to a signal of ten to the sixth power of the original Raman signal, indicating that a double layer of lipid has occurred on the surface. The membrane-modified nanoporous silver structure 52 is adsorbed to the charged pesticide, which means that there is a charged pesticide in the sample solution to be detected; The Raman signal of the molecule to be detected in the nanoporous silver structure 52 modified by the lipid film is detected, and no amplified signal is found in the Raman signal, that is, no Raman signal is detected or the Raman signal is very weak, indicating that the surface The nanoporous silver structure 52 modified with bilayer lipid membrane does not adsorb charged pesticides, that is, there is no charged pesticides in the sample solution to be detected or the concentration of charged pesticides is too low to be calculated. The SERS substrate in the third detection chip 13 adopts a nanoporous silver structure 53 for adsorbing hydrophilic pesticides in the molecules to be detected. The nanoporous silver structure 53 adopts an original nanoporous silver structure that does not require surface modification 53. If the Raman signal of the molecule to be detected in the nanoporous silver structure 53 on the third detection chip 13 is detected by a hand-held Raman spectrometer, when the molecule to be detected is adsorbed to the corresponding property, such as in the nanoporous silver structure If the third detection chip 13 where 53 is located is adsorbed to the hydrophilic pesticide, the Raman signal will be amplified to the sixth power of ten times the original Raman signal, indicating that the nanoporous silver structure 53 is adsorbed to the hydrophilic pesticide , that is, there is a hydrophilic pesticide in the sample solution to be detected; if the Raman signal of the molecule to be detected in the nanoporous silver structure 53 on the third detection chip 13 is detected by a handheld Raman spectrometer, no Raman signal is found. If there is any amplified signal of the Mann signal, that is, no Raman signal is detected or the Raman signal is very weak, it means that the nanoporous silver structure 53 does not adsorb hydrophilic pesticides, that is, there is no hydrophilicity in the sample solution to be detected. Pesticide or hydrophilic pesticide concentrations are too low to be counted. By setting multiple channels, it is applied to the detection chip 1 of different SRS substrates, so as to detect the molecules to be detected in the sample solutions to be detected with different properties, and improve the detection efficiency.

In this example, a single-layer lipid film is deposited on the nanoporous silver structure 5 by the technique of constructing an ordered ultra-thin molecular film (Langmuir-Blodgett), so as to obtain a nanoporous surface modified by a single-layer lipid film Silver structures51. More specifically, the lipid monolayer film was deposited onto a nanoporous silver sheet by the Langmuir-Blodgett method, and the surface pressure of the lipid monolayer film could be measured by a Williams paper pair pressure sensor to obtain the desired monolayer lipid membrane.

In this embodiment, the steps of modifying the surface of the nanoporous silver structure to modify the monolayer lipid membrane include:

Drop a certain amount of lipid solution on the water surface through a Hamilton micro-syringe, and wait for the lipid solution to evaporate to form a lipid film;

Squeeze the lipid film against the lipid film at a preset speed until the lipid film bears the target surface pressure;

Pull the glass slide in the water and the nanoporous silver structure on the glass slide up at a specified speed until the nanoporous silver structure is exposed to the water surface, and then the nanoporous silver structure modified with a single layer of lipid film on the surface can be obtained 51.

In this embodiment, the step of modifying the surface of the nanoporous silver structure with a single-layer lipid film is as follows: first, the lipid solution is dropped on the water surface with a Hamilton microinjector, and the amount of the lipid solution dropped on the water surface can be Select according to actual needs, such as the height of 1 mm on the water surface, can also be adjusted according to actual needs, after the lipid solution evaporates for a preset time to form a lipid film, as in a specific embodiment, the lipid solution After evaporating for 15 minutes to form the desired lipid film, squeeze the lipid film at a constant speed of 5 mm per minute so that the lipid film is subjected to the target surface pressure. Wherein, the nanoporous silver sheet is pasted on the glass slide and clamped to the clip, and the clip is used to fix the nanoporous silver sheet on the glass slide. Then pull the glass slide up at a constant rate of 0.1 mm per minute until the silver piece is completely exposed to the water surface to achieve a monolayer lipid film modification, maintaining the surface of the nanoporous silver sheet on the glass slide during the monolayer lipid modification process Pressure does not change. In other embodiments, the glass slide in the water and the nanoporous silver structure on the glass slide can also be pulled up at other specified speeds until the nanoporous silver structure emerges from the water surface. In the present example, the illustrated water means deionized water.

In this embodiment, the steps of modifying the surface of the nanoporous silver structure to modify the monolayer lipid membrane include:

Drop a certain amount of lipid solution on the water surface through a Hamilton micro-syringe, and wait for the lipid solution to evaporate to form a lipid film;

Extruding the lipid film against the lipid film at a preset speed until the lipid film bears the target surface pressure;

Pull up the glass slide in the water and the nanoporous silver structure on the glass slide at a specified speed until the nanoporous silver structure emerges from the water;

The nanoporous silver structure is inserted into the water again, so as to realize the nanoporous silver structure 52 with a double-layer lipid membrane modified on the surface.

In this embodiment, the step of modifying the surface of the nanoporous silver structure with a single-layer lipid film is as follows: first, the lipid solution is dropped on the water surface with a Hamilton microinjector, and the amount of the lipid solution dropped on the water surface can be Select according to actual needs, such as the height of 1 mm on the water surface, can also be adjusted according to actual needs, after the lipid solution evaporates for a preset time to form a lipid film, as in a specific embodiment, the lipid solution After evaporating for 15 minutes to form the desired lipid film, squeeze the lipid film at a constant speed of 5 mm per minute so that the lipid film is subjected to the target surface pressure. Wherein, the nanoporous silver sheet is pasted on the glass slide and clamped to the clip, and the clip is used to fix the nanoporous silver sheet on the glass slide. Then pull the glass slide up at a constant rate of 0.1 mm per minute until the silver piece is completely exposed to the water surface to achieve a monolayer lipid film modification, maintaining the surface of the nanoporous silver sheet on the glass slide during the monolayer lipid modification process Pressure does not change. In other embodiments, the glass slide in the water and the nanoporous silver structure on the glass slide can also be pulled up at other specified speeds until the nanoporous silver structure emerges from the water surface. Then the nanoporous silver structure was inserted into the water again, and a lipid film was set on the water surface in the above-mentioned way, and then the glass slide was pulled up at a constant rate of 0.1 mm per minute until the silver piece was completely exposed to the water surface to perform double layering. The lipid membrane is modified to realize a nanoporous silver structure modified by a bilayer lipid membrane on the surface, that is, a nanoporous silver structure with a double-layer surface modification52. In the present example, the illustrated water means deionized water.

The double-layer surface modification of the nanoporous silver structure is based on the single-layer surface modification, and the single-layer surface modification is carried out again to obtain the nanoporous silver structure 52 with the double-layer surface modification.

In this embodiment, the reducing agent is sodium borohydride solution. The sodium borohydride solution can be prepared by 0.1M sodium borohydride solution, and the 0.1 mole sodium borohydride solution can effectively reduce the silver plated with silver chloride to a nanoporous silver sheet.

In this embodiment, the input pipe end of the microfluidic pipeline 2 is set as a Y-shaped pipe, the chamber 3 where the sample solution to be tested is located communicates with the first port of the Y-shaped pipe, and the second port of the Y-shaped pipe communicates with the user. It communicates with the cavity 4 where the cleaning solution of the cleaning microfluidic pipeline 2 is located.

In this embodiment, a Y-shaped pipe is arranged at the input pipe end of the microfluidic pipeline 2, and the first port of the Y-shaped pipe is connected to the chamber 3 where the sample solution to be detected is located, and the second port of the Y-shaped pipe is It is connected to the cavity 4 where the cleaning solution is located, and a flow valve is provided at the junction of the Y-shaped pipeline, which is used for the output pipeline end of the microfluidic pipeline 2 to communicate with the sample solution to be tested, or the output of the microfluidic pipeline 2 The end of the pipeline is connected with the cleaning solution, which can effectively clean the microfluidic pipeline and realize reuse.

To sum up, for the detection device applied to the multi-channel SERS microfluidic chip provided in the embodiment of the present application, a plurality of detection chips 1 connected in parallel are provided, and a device for detecting corresponding properties is set on the detection chip 1. The nanoporous silver structure 5 of the molecules to be detected is used to solve the problems of difficult simultaneous detection and low detection efficiency of different properties to be detected, realize multi-channel simultaneous detection, and improve detection efficiency.

It should be noted that, in this document, the terms "comprising", "comprising" or any other variation thereof are intended to cover a non-exclusive inclusion such that a process, apparatus, article or method comprising a set of elements includes not only those elements, It also includes other elements that are not expressly listed, or that are inherent in the process, apparatus, article, or method. Without further limitations, an element defined by the phrase "comprising a ..." does not preclude the presence of additional same elements in the process, apparatus, article or method comprising the element.

The above descriptions are only preferred embodiments of the application, and are not intended to limit the patent scope of the application. Any equivalent structure or equivalent process conversion made by using the specification and drawings of the application, or directly or indirectly used in other relevant All technical fields are equally included in the patent protection scope of the present application.

Claims (9)

1. A detection device applied to a multi-channel SERS microfluidic chip, characterized in that it includes a microfluidic pipeline and a detection chip; The detection chip comprises a plurality, and is connected in parallel to the output pipeline end of the microfluidic pipeline, and the input pipeline end of the microfluidic pipeline is used to input the sample solution to be detected; Wherein, each of the detection chips includes a SERS substrate applied to detect substances of different properties to be detected, and the SERS substrate modifies the nano-silver structure for enriching and detecting samples of different properties to be detected, and the SERS The substrate includes any of the following nanoporous silver structures: a nanoporous silver structure modified with a single-layer lipid film on the surface, a nanoporous silver structure modified with a double-layer lipid film on the surface, and a single-layer lipid film on the surface The membrane-modified nanoporous silver structure is used for adsorption and detection of lipophilic pesticides, and the nanoporous silver structure modified with a bilayer lipid membrane on the surface is used for adsorption and detection of charged pesticides; The SERS substrate adsorbs the molecules to be detected in the sample solution to be detected, and detects the Raman signal of the molecules to be detected adsorbed on the detection chip by a handheld Raman spectrometer; The detection chip includes three, respectively the first detection chip, the second detection chip and the third detection chip; The SERS substrate in the first detection chip adopts a nanoporous silver structure modified with a monolayer lipid film on the surface; The SERS substrate in the second detection chip adopts a nanoporous silver structure with a bilayer lipid film modification on the surface; The SERS substrate in the third detection chip adopts the nanoporous silver structure. 2. the detection device that is applied to the SERS microfluidic chip of multichannel according to claim 1, is characterized in that, the manufacturing process of described SERS substrate comprises: The silver flakes with a purity of 99.9% are washed with double deionized water, and the washed silver flakes are placed in potassium chloride water with a preset concentration for electrochemical reaction to obtain silver flakes plated with silver chloride; Stirring the solution in which the silver chloride-plated silver flakes are located, placing the silver chloride-plated silver flakes in a reducing agent, and reducing them to nanoporous silver flakes by the reducing agent in situ; The porous silver sheet was washed with deionized water and ethanol to obtain a nanoporous silver structure. 3. The detection device applied to a multi-channel SERS microfluidic chip according to claim 2, wherein the electrochemical reaction is carried out to potassium chloride water by a voltage of 0.15 volts. 4. The detection device applied to a multi-channel SERS microfluidic chip according to any one of claims 1-3, wherein the diameter of each pore in the nanoporous silver structure is 60-80 nm. 5. according to the detection device of the SERS microfluidic chip that is applied to multichannel according to any one of claim 1-4, it is characterized in that, by the technology of building ordered ultrathin molecular film, monolayer lipid film is deposited on on the nanoporous silver structure to obtain a nanoporous silver structure modified with a monolayer lipid film on the surface. 6. the detection device that is applied to the SERS microfluidic chip of multichannel according to claim 5, is characterized in that, the step that the surface modification of described nanoporous silver structure occurs monolayer lipid film modification comprises: A certain amount of lipid solution is dropped on the water surface through a Hamilton micro-syringe, and the lipid solution is evaporated to form a lipid film; Squeezing the lipid film against the lipid film at a preset speed until the lipid film bears a target surface pressure; The glass slide in the water and the nanoporous silver structure on the glass slide are pulled up at a specified speed until the nanoporous silver structure is exposed to the water surface, and a monolayer of lipid on the surface can be obtained. Membrane-modified nanoporous silver structures. 7. the detection device that is applied to the SERS microfluidic chip of multichannel according to claim 5, is characterized in that, the step that the surface modification of described nanoporous silver structure occurs monolayer lipid film modification comprises: A certain amount of lipid solution is dropped on the water surface through a Hamilton micro-syringe, and the lipid solution is evaporated to form a lipid film; Squeezing the lipid film against the lipid film at a preset speed until the lipid film bears a target surface pressure; Pulling up the glass slide in the water and the nanoporous silver structure on the glass slide at a specified speed until the nanoporous silver structure is exposed to the water surface; The nanoporous silver structure is inserted into the water again, so as to realize the nanoporous silver structure with double lipid membrane modification on the surface. 8. The detection device applied to a multi-channel SERS microfluidic chip according to claim 2, wherein the reducing agent is a sodium borohydride solution. 9. the detection device that is applied to the SERS microfluidic chip of multichannel according to claim 1, is characterized in that, the input pipeline end of described microfluidic pipeline is set to Y-shaped pipeline, and described sample solution to be detected The cavity where it is located communicates with the first port of the Y-shaped pipeline, and the second port of the Y-shaped pipeline communicates with the cavity where the cleaning solution for cleaning the microfluidic pipeline is located.

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