CN115060779B - Blood anti-tumor drug metabolite concentration detection device and monitoring method thereof - Google Patents
Blood anti-tumor drug metabolite concentration detection device and monitoring method thereof Download PDFInfo
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- 229940041181 antineoplastic drug Drugs 0.000 title claims abstract description 40
- 239000002207 metabolite Substances 0.000 title claims abstract description 33
- 238000001514 detection method Methods 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims description 7
- 238000012544 monitoring process Methods 0.000 title claims description 3
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- 238000010521 absorption reaction Methods 0.000 claims abstract description 9
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- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
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- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
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Abstract
The invention discloses a blood anti-tumor drug metabolite concentration detection device, which comprises a carrying unit and a detection unit; the carrying unit comprises a first film roll, a second film roll, a supporting block, a liquid collecting tank and a first conveying pipe, and the detection unit comprises a blood electromagnetic pump, a first electrode, a second electrode, a third electrode, an antibody liquid box, a buffer liquid box and a dissolving liquid box; according to the invention, a first precipitate is generated by a specific immunoreaction of an antitumor drug metabolite in blood and an antibody liquid on a second reaction film layer, the first precipitate is separated under the electrophoresis action of a second electrode, the first precipitate is dissolved by a dissolving solution to obtain a first dissolving solution, the first dissolving solution is concentrated between two third electrodes under the electrophoresis action of the first electrode, the concentration of the antitumor drug metabolite in the blood is judged by measuring the conductivity between the two third electrodes, the detection of the absorption degree of the antitumor drug is realized, and the treatment effect is improved.
Description
Technical Field
The invention relates to a device for detecting the concentration of metabolites of blood anti-tumor drugs.
Background
After the oral antitumor drug is taken orally, some patients have serious adverse reactions such as nausea, vomiting and the like, so that the orally taken drug is completely or partially vomited; at present, a detection device special for detecting the absorption degree of the anti-tumor medicine is not provided, and after a patient vomits after taking the medicine, a doctor cannot judge the medicine absorption condition, so that the treatment opportunity is delayed, and the treatment effect is influenced.
Disclosure of Invention
The invention aims to overcome the defects and provide a device for detecting the concentration of metabolites of blood anti-tumor drugs.
In order to achieve the purpose, the invention adopts the following specific scheme:
the utility model provides a blood anti-tumor drug metabolite concentration detection device, includes and carries the unit and locate the detecting element who carries on the unit, carry the unit and detect the unit cooperation and detect the concentration of anti-tumor drug's in blood metabolite.
The carrier tape unit further comprises a first film roll, a second film roll, a supporting block, a liquid collecting tank and a first conveying pipe, wherein the first film roll and the second film roll are both nitrocellulose membranes with porous structures, the first film roll is provided with a first reaction film layer, the second film roll is provided with a second reaction film layer, the supporting block is arranged below the first reaction film layer, the liquid collecting tank is arranged below the second reaction film layer, one end of the first conveying pipe is connected with the liquid collecting tank, and the other end of the first conveying pipe extends to the upper side of the first reaction film layer;
the detection unit comprises a blood electromagnetic pump, first electrodes, second electrodes, third electrodes, an antibody liquid box, a buffer liquid box and a dissolving liquid box, wherein the blood electromagnetic pump is used for conveying blood to a second reaction film layer, the two first electrodes are arranged right above the first reaction film layer at intervals, the two second electrodes are arranged right above the second reaction film layer at intervals, the two third electrodes are arranged right above the second reaction film layer at intervals and correspond to the positions of supporting blocks, the antibody liquid box is used for providing antibody liquid capable of performing specific immune reaction with metabolites of antitumor drugs and generating first precipitates for the second reaction film layer, and antibody protein in the antibody liquid is linked with a group capable of improving the conductivity of the buffer liquid and presenting electronegativity; the buffer solution box is used for providing a buffer solution with stable pH value for the first reaction film layer and the second reaction film layer, and the dissolving solution box is used for providing a dissolving solution capable of dissolving the first precipitate for the second reaction film layer.
The detection unit further comprises a first mounting seat and a first electrode seat, wherein first push rods are mounted on two sides of the first mounting seat, and the first electrode seat is fixedly connected with output ends of the two first push rods; two first electrodes which are oppositely arranged at intervals are arranged at the position, corresponding to the first reaction film layer, of the bottom surface of the first electrode seat, and two second electrodes which are oppositely arranged at intervals are arranged at the position, corresponding to the second reaction film layer, of the bottom surface of the first electrode seat; the first electrode and the second electrode both penetrate through the first mounting seat in a downward movable manner; the first electrode and the second electrode are both provided with a first dripping hole;
the top of the first electrode holder is provided with an antibody liquid box and a buffer liquid box; a first quantitative pump is installed at a liquid outlet of the buffer liquid box, and a second quantitative pump is installed at a liquid outlet of the antibody liquid box; a first liquid channel, a second liquid channel and a third liquid channel are formed in the first electrode seat, the first liquid channel is communicated with first dripping holes and first quantitative pumps on the two first electrodes, the second liquid channel is communicated with first dripping holes and second quantitative pumps on the two second electrodes, two ends of the third liquid channel are respectively communicated with the first liquid channel and the second liquid channel, and a control valve used for controlling the communication of the first liquid channel and the second liquid channel is arranged on the third liquid channel;
the first mounting seat is arranged at a position corresponding to the position of the first reaction film layer, a second push rod and a second electrode seat are arranged between the two first electrodes, the second electrode seat penetrates through the first mounting seat in a sliding mode and is connected with the output end of the second push rod, and third electrodes are arranged on two sides of the second electrode seat;
the first mounting seat is provided with a third push rod at a position corresponding to the liquid collecting tank and is provided with a dripping head in a sliding penetrating manner, the dripping head is connected with the output end of the third push rod, and the bottom of the dripping head is provided with a plurality of rows of second dripping holes and a plurality of rows of third dripping holes in a staggered manner;
a dissolving liquid box is further mounted at the top of the first electrode holder, a third quantitative pump is mounted at a liquid outlet of the dissolving liquid box, and the third quantitative pump is communicated with each second dripping hole through a second conveying pipe;
the blood electromagnetic pump is further connected with a blood suction needle which can be connected with an interface end of an external vein retention needle in an inserting mode.
Furthermore, a first boss is arranged on one side of the bottom of the first electrode, which is far away from the other first electrode, in a downward protruding manner, and a second boss is arranged on one side of the bottom of the second electrode, which is far away from the other second electrode, in a downward protruding manner.
Furthermore, third bosses are convexly arranged on two sides of the dripping head respectively, and the second dripping holes in the multiple rows and the third dripping holes in the multiple rows are positioned between the two third bosses.
The blood electromagnetic pump further comprises a pump body, wherein a liquid suction port and a liquid discharge port are formed in two ends of the pump body, the blood suction needle is connected with the liquid suction port, and the third conveying pipe is connected with the liquid discharge port; the magnetic pump is characterized in that a first groove is formed in the middle of the pump body, two oppositely arranged diaphragms are fixedly mounted in the first groove, magnetic coatings are coated on the outer side surfaces of the diaphragms, the polarities of the corresponding magnetic coatings between the two diaphragms are the same, second grooves are formed in the two sides of the first groove of the pump body, magnetic shielding frames are slidably mounted in the second grooves, electromagnets are mounted on the two sides of each second groove, a cover plate is fixed to the top of the first groove, and a sealed liquid suction cavity is formed among the cover plate, the bottom of the first groove and the two diaphragms; the top of the pump body is provided with a linear motor, and the output end of the linear motor is connected with the magnetic shielding frame.
The carrier tape unit further comprises two side plates which are arranged in parallel, wherein a first motor is arranged on the outer side of one side plate; two first reels are rotationally connected between the two side plates at intervals, and one of the first reels is in transmission connection with the output end of a first motor; the two side plates are further rotatably connected with two first supporting shafts at intervals between the two first reels, the first supporting shafts are located above the sides of the first reels and located right below the corresponding first electrodes and the corresponding second electrodes, the two ends of the first reels are correspondingly sleeved with first film rolls and second film rolls, the middle parts of the first film rolls are arranged at one ends of the first supporting shafts in a spanning mode, and the middle parts of the second film rolls are arranged at the other ends of the first supporting shafts in a spanning mode; the first film roll part positioned between the two first supporting shafts is a first reaction film layer, and the second film roll part positioned between the two first supporting shafts is a second reaction film layer; the supporting blocks are correspondingly and fixedly arranged on the side plates; the liquid collecting grooves are correspondingly and fixedly arranged on the side plates; two fixed mounting has the flexible pad with patient's wrist shape looks adaptation between the bottom of curb plate, the fixed wrist strap that is used for flexible pad and patient's wrist to fix that is provided with in the below of flexible pad.
Further, the diameter of one end of the first supporting shaft is larger than that of the other end of the first supporting shaft; the middle part of the first film roll is arranged at the small-diameter end of the first supporting shaft in a spanning mode, and the middle part of the second film roll is arranged at the large-diameter end of the first supporting shaft in a spanning mode.
Further, the first roll of film has a thickness and width greater than the thickness and width of the second roll of film.
Furthermore, the left side and the right side of the supporting block are respectively provided with a first boss, the positions of the two first bosses correspond to the positions of the two third electrodes, and the top surface of each first boss is in contact with the lower surface of the first reaction film layer.
The beneficial effects of the invention are as follows: the method comprises the steps of arranging a first reaction film layer and a second reaction film layer, dropwise adding buffer solution on the two reaction film layers to provide an electrophoresis environment, providing two reaction carriers, carrying out specific immunoreaction on an antitumor drug metabolite in blood and an antibody liquid on the second reaction film layer to generate a first precipitate, separating the first precipitate under the electrophoresis action of a second electrode, dissolving the first precipitate by using a dissolving solution to obtain a first dissolving solution, dropwise adding the obtained first dissolving solution onto the first reaction film layer, and concentrating the first dissolving solution between two third electrodes under the electrophoresis action of the first electrode, so that the concentration of the antitumor drug metabolite in the blood is judged by measuring the conductivity between the two third electrodes, the detection of the absorption degree of the antitumor drug is realized, and the treatment effect is improved.
Drawings
FIG. 1 is a perspective view provided by an embodiment of the present invention;
FIG. 2 is a perspective view of a portion of the structure provided by an embodiment of the present invention;
FIG. 3 is a perspective view of another perspective of a portion of the structure provided by an embodiment of the present invention;
FIG. 4 is a perspective view of a detection unit provided by an embodiment of the present invention;
FIG. 5 is a perspective view of another perspective of a detecting unit provided in an embodiment of the present invention;
FIG. 6 is an exploded view of a detection unit provided in an embodiment of the present invention;
FIG. 7 is a perspective view of a blood solenoid pump provided by an embodiment of the present invention;
FIG. 8 is a schematic cross-sectional view of a blood electromagnetic pump according to an embodiment of the present invention;
fig. 9 is a schematic structural diagram of a first electrode holder provided in an embodiment of the present invention;
FIG. 10 is a schematic structural diagram of a dropping head provided in an embodiment of the present invention;
fig. 11 is a perspective view of a carrier tape unit provided by an embodiment of the present invention;
description of reference numerals: 100. a carrier tape unit; 101. a side plate; 102. a first motor; 103. a first reel; 104. a first support shaft; 105. a flexible pad; 106. a wristband; 107. a first roll of film; 108. a second roll of film; 109. a supporting block; 110. a liquid collecting tank; 111. a first delivery pipe;
200. a detection unit; 201. a first mounting seat; 202. a first electrode holder; 203. a first push rod; 204. a blood electromagnetic pump; 2041. a pump body; 2042. a membrane; 2043. a magnetic coating; 2044. a magnetic shield frame; 2045. an electromagnet; 2046. a cover plate; 2047. a linear motor; 205. a first electrode; 206. a second electrode; 207. a third electrode; 208. antibody liquid box; 209. a buffer solution box; 210. a solution box; 211. a first fixed displacement pump; 212. a second fixed displacement pump; 213. a control valve; 214. a second push rod; 215. a second electrode holder; 216. a third push rod; 217. a dropping head; 218. a third fixed displacement pump; 219. a second delivery pipe; 220. a third delivery pipe; 221. blood sucking needle.
Detailed Description
The invention will be described in further detail with reference to the following figures and specific examples, without limiting the scope of the invention.
As shown in fig. 1 to fig. 11, the blood anti-tumor drug metabolite concentration detection apparatus of the present embodiment includes a carrying unit 100 and a detection unit 200 disposed on the carrying unit 100, wherein the carrying unit 100 and the detection unit 200 cooperate to detect the concentration of the anti-tumor drug metabolite in blood.
In this embodiment, the carrier tape unit 100 includes a first film roll 107, a second film roll 108, a supporting block 109, a liquid collecting tank 110, and a first conveying pipe 111, where the first film roll 107 and the second film roll 108 are both nitrocellulose membranes with porous structures, the first film roll 107 has a first reaction film layer, the second film roll 108 has a second reaction film layer, the supporting block 109 is disposed below the first reaction film layer, the liquid collecting tank 110 is disposed below the second reaction film layer, one end of the first conveying pipe 111 is connected to the liquid collecting tank 110, and the other end of the first conveying pipe 111 extends to above the first reaction film layer;
the detection unit 200 comprises a blood electromagnetic pump 204, a first electrode 205, a second electrode 206, a third electrode 207, an antibody liquid box 208, a buffer liquid box 209 and a dissolving liquid box 210, wherein the blood electromagnetic pump 204 is used for conveying blood to a second reaction film layer, the two first electrodes 205 are arranged right above the first reaction film layer at intervals, the two second electrodes 206 are arranged right above the second reaction film layer at intervals, the two third electrodes 207 are arranged right above the second reaction film layer at intervals and correspond to the supporting block 109 in position, the antibody liquid box 208 is used for providing an antibody liquid which can generate specific immunoreaction with an antitumor drug metabolite and generate a first precipitate to the second reaction film layer, and an antibody protein in the antibody liquid is linked with a group which can improve the conductivity of the buffer liquid and is electronegative; the buffer box 209 is used for providing a buffer solution with a stable pH value for the first reaction membrane layer and the second reaction membrane layer, and the lysis solution box 210 is used for providing a lysis solution for the second reaction membrane layer, wherein the lysis solution can dissolve the first precipitate.
The working mode of the embodiment is as follows: when the antibody liquid box works, the two first electrodes 205 are in compression joint with the first reaction film layer, the two second electrodes 206 are in compression joint with the second reaction film layer, the buffer liquid in the buffer liquid box 209 is dripped on the first reaction film layer and the second reaction film layer, the buffer liquid is diffused to the whole first reaction film layer and the whole second reaction film layer along gaps under the siphoning action due to the fact that the first reaction film layer and the second reaction film layer are made of porous materials, and then the antibody liquid in the antibody liquid box 208 is placed on the dripped second reaction film layer;
then, blood of a patient is dripped on the second reaction film layer through the blood electromagnetic pump 204, the metabolite of the antitumor drug in the blood and the antibody liquid generate specific immunoreaction at the moment and generate a first precipitate, after the reaction is finished, the second electrode 206 is electrified to carry out electrophoresis on the second reaction film layer, and because the free antibody is electronegative, the free antibody gradually gathers to the positive end of the second electrode 206 in the electrophoresis process, so that the free antibody protein around the first precipitate is removed; after electrophoresis is finished, the dissolving solution in the dissolving solution box 210 is dripped on the area of the second reaction film layer corresponding to the liquid collecting tank 110, the first precipitate is dissolved into a free state under the action of the dissolving solution to obtain a first solution, the first solution penetrates the second reaction film layer and then enters the liquid collecting tank 110, the first solution in the liquid collecting tank 110 is dripped on the surface of the first reaction film layer through the first conveying pipe 111, after the first dissolving solution is completely transferred onto the first reaction film layer, the first electrode 205 is electrified to carry out electrophoresis on the first dissolving solution on the first reaction film layer, the electrophoresis voltage is adjusted according to the electronegativity strength of the buffer solution, when electrophoresis is stopped, the first dissolving solution concentrated distribution area is positioned in the area between the two third electrodes 207, then the two third electrodes 207 are pressed against the first reaction film layer and electrified, the conductivity between the two third electrodes 207 is measured, the concentration of the metabolite of the antitumor drug in blood is judged according to realize the detection of the absorption degree of the antitumor drug, so as to guide whether to take a supplement drug after vomiting.
In this embodiment, a first reaction membrane layer and a second reaction membrane layer are arranged, and a buffer solution is dropped on both the two reaction membrane layers to provide an electrophoresis environment, so as to provide two reaction carriers, then a specific immunoreaction is performed between an antitumor drug metabolite in blood and an antibody liquid on the second reaction membrane layer to generate a first precipitate, the first precipitate is separated under the electrophoresis action of the second electrode 206, then the first precipitate is dissolved by a dissolving solution to obtain a first dissolving solution, the obtained first dissolving solution is dropped on the first reaction membrane layer, the first dissolving solution is concentrated between the two third electrodes 207 under the electrophoresis action of the first electrode 205, and thus, the concentration of the antitumor drug metabolite in the blood is determined by measuring the conductivity between the two third electrodes 207, so as to achieve the detection of the absorption degree of the antitumor drug, and facilitate a doctor to know the drug absorption condition, thereby facilitating the improvement of the therapeutic effect.
In the embodiment, the interference of other factors to the detection structure can be eliminated by carrying out electrophoresis impurity removal twice, and higher detection precision is obtained.
Based on the above embodiment, further, the detection unit 200 further includes a first mounting seat 201 and a first electrode 205 seat 202, wherein the first push rods 203 are mounted on two sides of the first mounting seat 201, and the first electrode 205 seat 202 is fixedly connected to output ends of the two first push rods 203; two first electrodes 205 which are oppositely arranged at intervals are arranged at the positions corresponding to the first reaction film layer on the bottom surface of the first electrode 205 seat 202, and two second electrodes 206 which are oppositely arranged at intervals are arranged at the positions corresponding to the second reaction film layer; the first electrode 205 and the second electrode 206 both penetrate through the first mounting seat 201 in a downward moving manner; the first electrode 205 and the second electrode 206 are both provided with a first dripping hole;
an antibody liquid box 208 and a buffer liquid box 209 are arranged on the top of the first electrode 205 seat 202; a first quantitative pump 211 is installed at the liquid outlet of the buffer liquid box 209, and a second quantitative pump 212 is installed at the liquid outlet of the antibody liquid box 208; a first liquid channel, a second liquid channel and a third liquid channel are formed in the first electrode 205 seat 202, the first liquid channel is communicated with first dripping holes and first quantitative pumps 211 on the two first electrodes 205, the second liquid channel is communicated with first dripping holes and second quantitative pumps 212 on the two second electrodes 206, two ends of the third liquid channel are respectively communicated with the first liquid channel and the second liquid channel, and a control valve 213 for controlling the communication between the first liquid channel and the second liquid channel is arranged on the third liquid channel;
the first mounting seat 201 is provided with a second push rod 214 and a second electrode 206 seat at a position corresponding to the position of the first reaction film layer and between the two first electrodes 205, the second electrode 206 seat is slidably arranged through the first mounting seat 201 and connected with the output end of the second push rod 214, and both sides of the second electrode 206 seat are provided with third electrodes 207;
a third push rod 216 and a dropping head 217 are slidably arranged in the first mounting seat 201 in a penetrating manner at a position corresponding to the liquid collecting tank 110, the dropping head 217 is connected with the output end of the third push rod 216, and a plurality of rows of second dropping holes and a plurality of rows of third dropping holes are formed in the bottom of the dropping head 217 in a staggered manner;
a dissolving liquid box 210 is further installed on the top of the seat 202 of the first electrode 205, a liquid outlet of the dissolving liquid box 210 is provided with a third quantitative pump 218, and the third quantitative pump 218 is communicated with each second dripping hole through a second conveying pipe 219;
the first mounting seat 201 is further provided with a blood electromagnetic pump 204, the blood electromagnetic pump 204 is communicated with each third dripping hole through a third conveying pipe 220, and the blood electromagnetic pump 204 is further connected with a blood suction needle 221 which can be inserted into an interface end of an external vein retention needle.
When the blood sucking needle is actually used, the blood sucking needle 221 is inserted into an interface of an external venous indwelling needle, the first push rod 203 pushes the first electrode 205 seat 202 to drive the first electrode 205 and the second electrode 206 to move downwards, so that the first electrode 205 is in compression joint with a first reaction film layer, the second electrode 206 is in compression joint with a second reaction film layer, the buffer solution in the buffer solution box 209 is pumped into the second liquid channel by the first quantitative pump 211, the valve 213 is controlled to be opened at the same time, the second liquid channel is communicated with the first liquid channel through the third liquid channel, the buffer solution is correspondingly dripped into the first reaction film layer and the second reaction film layer through the first dripping holes on the first electrode 205 and the second electrode 206 respectively through the second liquid channel, the third liquid channel and the first liquid channel, and the buffer solution is simultaneously pumped into the first reaction film layer and the second reaction film layer; after the completion, the control valve 213 is closed, so that the first liquid channel is not communicated with the second liquid channel, the antibody liquid in the antibody liquid box 208 is pumped into the second liquid channel by the second quantitative pump 212 and is dripped onto the second reaction film layer through the first dripping hole on the second electrode 206, so that the quantitative conveying of the antibody liquid is realized, after the completion, the blood electromagnetic pump 204 works, the blood of the patient is conveyed into the dripping head 217 through the third conveying pipe 220 and is dispersedly dripped onto the second reaction film layer through each third dripping hole in the dripping head 217, and after the dripping is completed, the anti-tumor drug metabolite in the blood and the antibody liquid generate specific immunoreaction and generate a first precipitate; after the reaction is finished, the second electrode 206 is electrified, and the second reaction film layer is subjected to electrophoresis, so that the free antibody protein around the first precipitate is removed; after electrophoresis is completed, the third push rod 216 drives the dropping head 217 to move downwards, so that the dropping head 217 is pressed on the second reaction film layer under the support of the liquid collecting tank 110, then the third quantitative pump 218 pumps the solution in the solution box 210 into each second dropping hole through the second conveying pipe 219, the solution is dispersed and dropped in the corresponding area of the second reaction film layer through each second dropping hole, so as to form a first solution, the second solution penetrates through the second reaction film layer and enters the liquid collecting tank 110 to be collected, the second solution is transferred onto the first reaction film layer through the first conveying pipe 111, then the first solution is concentrated and collected in the corresponding area between the two third electrodes 207 through the electrophoresis action of the first electrode 205, then the second push rod 214 drives the two third electrodes 207 to downwards stretch to be in contact conduction with the first reaction film layer, and the conductivity between the two third electrodes 207 is measured under the electrification of the third electrode 207, so as to judge the concentration of the metabolite of the antitumor drug in the blood, and realize the detection of the absorption degree of the antitumor drug.
Based on the above embodiment, further, a first protrusion is protruded downward from a side of the bottom of the first electrode 205 away from the other first electrode 205, and a second protrusion is protruded downward from a side of the bottom of the second electrode 206 away from the other second electrode 206.
Based on the above embodiment, further, third bosses are respectively convexly provided at two sides of the dropping head 217, and the multiple rows of second dropping holes and the multiple rows of third dropping holes are located between the two third bosses. This embodiment sets up the third boss through the both sides at dropwise add head 217 to when probing down with the second reaction rete contact, isolated second reaction rete is located the region between two third bosses at dropwise add head 217, so that the first solution that forms concentrates on permeating to the collecting tank 110 between two third bosses in, make first solution can fully collect.
Based on the above embodiment, further, the blood electromagnetic pump 204 includes a pump body 2041, a fluid suction port and a fluid discharge port are formed at two ends of the pump body 2041, the blood suction needle 221 is connected to the fluid suction port, and the third delivery pipe 220 is connected to the fluid discharge port; a first groove is formed in the middle of the pump body 2041, two oppositely arranged diaphragms 2042 are fixedly installed in the first groove, magnetic coatings 2043 are coated on the outer side surfaces of the diaphragms 2042, the polarities of the corresponding magnetic coatings 2043 between the two diaphragms 2042 are the same, second grooves are formed in the two sides of the first groove of the pump body 2041, magnetic shielding frames 2044 are installed in the second grooves in a sliding mode, electromagnets 2045 are installed on the two sides of each second groove, a cover plate 2046 is fixed to the top of the first groove, and a sealed liquid suction cavity is formed among the cover plate 2046, the bottom of the first groove and the two diaphragms 2042; the top of the pump body 2041 is provided with a linear motor 2047, and the output end of the linear motor 2047 is connected with a magnetic shielding frame 2044.
When the blood sucking and sucking device is in actual use, the electromagnet 2045 is electrified to generate a magnetic field with the same polarity as that of the magnetic coating 2043, the diaphragms 2042 on the two sides are pressed inwards against the magnetic coating 2043 to enable the volume of the liquid sucking cavity to be zero, the blood electromagnetic pump 204 does not work, when the linear motor 2047 drives the magnetic shielding frame 2044 to move between the magnetic coating 2043 and the electromagnet 2045, the magnetic shielding frame 2044 shields the magnetic field generated by the electromagnet 2045 in the area, and due to the fact that the opposite magnetic coatings 2043 on the diaphragms 2042 on the two sides are the same in polarity, the two diaphragms 2042 are separated towards the two sides under the action of the magnetic field repulsive force of the magnetic coatings 2043 on the two sides, the volume of the liquid sucking cavity is increased, the pressure intensity is reduced, blood in a human body is sucked into the liquid sucking cavity through the liquid sucking port and the blood sucking needle 221, then the linear motor 2047 drives the magnetic shielding frame 2044 to move towards the liquid discharging port, the position of the liquid sucking cavity is correspondingly changed towards the liquid discharging port, and the blood is transferred until the blood in the liquid discharging port is discharged into the third liquid discharging pipe 220 through the conveying pipe 217; in the blood sucking and transferring process, the membrane 2042 is in a flexible and bending state, and does not generate shearing action on blood to destroy blood cells.
Based on the above embodiment, further, the carrier tape unit 100 further includes two side plates 101 disposed in parallel, wherein a first motor 102 is mounted on an outer side of one of the side plates 101; two first reels 103 are rotatably connected between the two side plates 101 at intervals, wherein one first reel 103 is in transmission connection with the output end of a first motor 102; the two side plates 101 are further rotatably connected with two first supporting shafts 104 at intervals between the two first reels 103, the first supporting shafts 104 are located above the sides of the first reels 103 and under the corresponding first electrodes 205 and second electrodes 206, the two ends of the first reels 103 are correspondingly sleeved with a first film roll 107 and a second film roll 108, the middle of the first film roll 107 spans one end of the first supporting shaft 104, and the middle of the second film roll 108 spans the other end of the first supporting shaft 104; the first film roll 107 part between the two first support shafts 104 is a first reaction film layer, and the second film roll 108 part between the two first support shafts 104 is a second reaction film layer; the supporting blocks 109 are correspondingly and fixedly arranged on the side plates 101; the liquid collecting groove 110 is correspondingly and fixedly arranged on the side plate 101; a flexible cushion 105 matched with the wrist of the patient in shape is fixedly installed between the bottoms of the two side plates 101, and a wrist strap 106 used for fixing the flexible cushion 105 and the wrist of the patient is fixedly arranged below the flexible cushion 105.
During the in-service use, fix whole detection device in patient's wrist department through wrist strap 106, so that blood suction needle 221 and external vein are kept somewhere the needle interface and are pegged graft, high durability and convenient installation, drive first spool 103 through first motor 102 simultaneously and rotate, drive first membrane book 107 and second membrane book 108 and receive and unreel the operation simultaneously, so that will use the first reaction rete and the second reaction rete of accomplishing to roll, place next first reaction rete and second reaction rete in between two first supporting shafts 104 simultaneously, so as to carry out next detection operation, so realize the detection of continuous many rounds.
Based on the above embodiment, further, the diameter of one end of the first supporting shaft 104 is larger than that of the other end thereof; the first roll 107 of film spans the small diameter end of the first support shaft 104 in the middle and the second roll 108 of film spans the large diameter end of the first support shaft 104 in the middle. In the embodiment, a height difference is formed between the two ends of the first support shaft 104, so that a height difference is formed between the second reaction film layer and the second reaction film layer, and the first solution can sufficiently flow to the first reaction film layer through the first delivery pipe 111.
Based on the above embodiment, further, the thickness and the width of the first film roll 107 are both larger than those of the second film roll 108. In this embodiment, the thickness of the first reaction film layer and the thickness and the width of the second reaction film layer are set so that the first solution can more easily permeate the second reaction film layer to enter the liquid collecting tank 110.
Based on the above embodiment, further, the left and right sides of the supporting block 109 are respectively provided with first bosses, the positions of the two first bosses correspond to the positions of the two third electrodes 207, and the top surfaces of the first bosses contact with the lower surface of the first reaction film layer. In the embodiment, the first boss is matched with the two third electrodes 207, so that the part of the first reaction film layer between the two third electrodes 207 is blocked and isolated, the conductivity of the first solution on the part of the first reaction film layer is measured, interference factors are reduced, and the measurement precision is higher.
The above description is only a preferred embodiment of the present invention, and all equivalent changes or modifications of the structure, characteristics and principles described in the present patent application are included in the protection scope of the present patent application.
Claims (8)
1. The device for detecting the concentration of the metabolite of the blood anti-tumor drug is characterized by comprising a carrying unit (100) and a detection unit (200) arranged on the carrying unit (100), wherein the carrying unit (100) is matched with the detection unit (200) to detect the concentration of the metabolite of the anti-tumor drug in the blood;
the carrier tape unit (100) comprises a first film roll (107), a second film roll (108), a supporting block (109), a liquid collecting tank (110) and a first conveying pipe (111), wherein the first film roll (107) and the second film roll (108) are both nitrocellulose membranes with porous structures, the first film roll (107) is provided with a first reaction film layer, the second film roll (108) is provided with a second reaction film layer, the supporting block (109) is arranged below the first reaction film layer, the liquid collecting tank (110) is arranged below the second reaction film layer, one end of the first conveying pipe (111) is connected with the liquid collecting tank (110), and the other end of the first conveying pipe (111) extends to the upper part of the first reaction film layer;
the detection unit (200) comprises a blood electromagnetic pump (204), first electrodes (205), second electrodes (206), third electrodes (207), an antibody liquid box (208), a buffer liquid box (209) and a dissolving liquid box (210), wherein the blood electromagnetic pump (204) is used for conveying blood to a second reaction film layer, the two first electrodes (205) are arranged above the first reaction film layer at intervals, the two second electrodes (206) are arranged above the second reaction film layer at intervals, the two third electrodes (207) are arranged above the second reaction film layer at intervals and correspond to the supporting block (109), the antibody liquid box (208) is used for providing antibody liquid which can generate specific immune reaction with an antitumor drug metabolite and generate first precipitates for the second reaction film layer, and groups which can improve the conductivity of a buffer liquid and are electronegative are linked to antibody protein in the antibody liquid; the buffer box (209) is used for providing buffer solution with stable pH value for the first reaction film layer and the second reaction film layer, and the dissolving solution box (210) is used for providing dissolving solution for dissolving the first precipitate for the second reaction film layer;
the detection unit (200) further comprises a first mounting seat (201) and first electrode (205) seats (202), first push rods (203) are mounted on two sides of the first mounting seat (201), and the first electrode (205) seats (202) are fixedly connected with output ends of the two first push rods (203); two first electrodes (205) which are oppositely arranged at intervals are arranged at the positions corresponding to the first reaction film layer on the bottom surface of the first electrode (205) seat (202), and two second electrodes (206) which are oppositely arranged at intervals are arranged at the positions corresponding to the second reaction film layer; the first electrode (205) and the second electrode (206) both penetrate through the first mounting seat (201) in a downward movable manner; the first electrode (205) and the second electrode (206) are both provided with a first dripping hole;
an antibody liquid box (208) and a buffer liquid box (209) are arranged on the top of the first electrode (205) seat (202); a first quantitative pump (211) is installed at a liquid outlet of the buffer liquid box (209), and a second quantitative pump (212) is installed at a liquid outlet of the antibody liquid box (208); a first liquid channel, a second liquid channel and a third liquid channel are formed in a seat (202) of the first electrode (205), the first liquid channel is communicated with first dripping holes and first quantitative pumps (211) on the two first electrodes (205), the second liquid channel is communicated with first dripping holes and second quantitative pumps (212) on the two second electrodes (206), two ends of the third liquid channel are respectively communicated with the first liquid channel and the second liquid channel, and a control valve (213) used for controlling the communication between the first liquid channel and the second liquid channel is arranged on the third liquid channel;
the first mounting seat (201) is provided with a second push rod (214) and a second electrode (206) seat between the two first electrodes (205) at a position corresponding to the position of the first reaction film layer, the second electrode (206) seat is slidably arranged on the first mounting seat (201) in a penetrating manner and is connected with the output end of the second push rod (214), and both sides of the second electrode (206) seat are provided with third electrodes (207);
a third push rod (216) and a dripping head (217) are slidably arranged in the first mounting seat (201) in a penetrating manner at a position corresponding to the liquid collecting tank (110), the dripping head (217) is connected with the output end of the third push rod (216), and a plurality of rows of second dripping holes and a plurality of rows of third dripping holes are formed in the bottom of the dripping head (217) in a staggered manner;
a dissolving liquid box (210) is further mounted at the top of the first electrode (205) seat (202), a third quantitative pump (218) is mounted at a liquid outlet of the dissolving liquid box (210), and the third quantitative pump (218) is communicated with each second dripping hole through a second conveying pipe (219);
blood electromagnetic pump (204) is still installed in first mount pad (201), blood electromagnetic pump (204) through third conveyer pipe (220) with each third dropwise add hole intercommunication, blood electromagnetic pump (204) still are connected with can keep somewhere blood suction needle (221) that needle interface end was pegged graft with external vein.
2. The device for detecting the metabolite concentration of a blood anti-tumor drug as claimed in claim 1, wherein a first boss is protruded downwards from one side of the bottom of the first electrode (205) far away from the other first electrode (205), and a second boss is protruded downwards from one side of the bottom of the second electrode (206) far away from the other second electrode (206).
3. The device for detecting the metabolite concentration of a hematologic antitumor drug according to claim 1, wherein third bosses are respectively protruded at both sides of the dripping head (217), and the plurality of rows of second dripping holes and the plurality of rows of third dripping holes are located between the two third bosses.
4. The device for detecting the metabolite concentration of a hematological antitumor drug according to claim 1, wherein the hematological electromagnetic pump (204) comprises a pump body (2041), a fluid suction port and a fluid discharge port are formed at two ends of the pump body (2041), the blood suction needle (221) is connected with the fluid suction port, and the third delivery pipe (220) is connected with the fluid discharge port; a first groove is formed in the middle of the pump body (2041), two oppositely arranged diaphragms (2042) are fixedly mounted in the first groove, magnetic coatings (2043) are coated on the outer side faces of the diaphragms (2042), the polarities of the corresponding magnetic coatings (2043) between the two diaphragms (2042) are the same, second grooves are formed in the two sides of the first groove of the pump body (2041), magnetic shielding frames (2044) are mounted in the second groove in a sliding mode, electromagnets (2045) are mounted on the two sides of each second groove, a cover plate (2046) is fixed to the top of each first groove, and sealed liquid suction cavities are formed among the cover plate (2046), the groove bottom of each first groove and the two diaphragms (2042); linear electric motor (2047) is installed at the top of the pump body (2041), and the output end of linear electric motor (2047) is connected with magnetic shielding frame (2044).
5. The apparatus for detecting the metabolite concentration of a hematological antitumor drug according to claim 1, wherein the carrier unit (100) further comprises two side plates (101) arranged in parallel, wherein a first motor (102) is installed on the outer side of one of the side plates (101); two first reels (103) are rotatably connected between the two side plates (101) at intervals, wherein one first reel (103) is in transmission connection with the output end of a first motor (102); the two side plates (101) are further rotatably connected with two first supporting shafts (104) between the two first reels (103) at intervals, the first supporting shafts (104) are located above the sides of the first reels (103) and located right below corresponding first electrodes (205) and second electrodes (206), two ends of the first reels (103) are correspondingly sleeved with first film rolls (107) and second film rolls (108), the middle of each first film roll (107) is spanned at one end of the corresponding first supporting shaft (104), and the middle of each second film roll (108) is spanned at the other end of the corresponding first supporting shaft (104); the part of the first film roll (107) positioned between the two first supporting shafts (104) is a first reaction film layer, and the part of the second film roll (108) positioned between the two first supporting shafts (104) is a second reaction film layer; the supporting blocks (109) are correspondingly and fixedly arranged on the side plates (101); the liquid collecting grooves (110) are correspondingly and fixedly arranged on the side plates (101); two fixed mounting has flexible pad (105) with patient's wrist shape looks adaptation between the bottom of curb plate (101), the fixed wrist strap (106) that is used for flexible pad (105) and patient's wrist to fix that is provided with in below of flexible pad (105).
6. The apparatus for detecting the metabolite concentration of a hematological antitumor drug according to claim 5, wherein the diameter of one end of the first supporting shaft (104) is larger than that of the other end thereof; the middle part of the first film roll (107) spans the small-diameter end of the first supporting shaft (104), and the middle part of the second film roll (108) spans the large-diameter end of the first supporting shaft (104);
the first roll (107) has a thickness and width greater than the thickness and width of the second roll (108).
7. The device for detecting the metabolite concentration of a hematologic antitumor drug according to claim 1, wherein the left side and the right side of the supporting block (109) are respectively provided with a first boss, the positions of the two first bosses correspond to the positions of the two third electrodes (207), and the top surface of each first boss contacts with the lower surface of the first reaction film layer.
8. A monitoring method using the apparatus for detecting a metabolite concentration of a hematological antitumor drug according to any one of claims 1 to 7, comprising the steps of:
s100: two first electrodes (205) are in pressure joint on the first reaction film layer, two second electrodes (206) are in pressure joint on the second reaction film layer, a buffer solution in a buffer solution box (209) is dripped on the first reaction film layer and the second reaction film layer, and then an antibody solution in an antibody solution box (208) is placed on the dripped second reaction film layer;
s200: then, blood of a patient is dripped on the second reaction film layer through a blood electromagnetic pump (204), at the moment, anti-tumor drug metabolites in the blood and antibody liquid generate specific immune reaction and generate a first precipitate, after the reaction is finished, the second electrode (206) is electrified to carry out electrophoresis on the second reaction film layer, and because the free antibody is electronegative, the free antibody gradually gathers towards the positive end of the second electrode (206) in the electrophoresis process, so that the free antibody protein around the first precipitate is removed;
s300: after electrophoresis is finished, the dissolving solution in the dissolving solution box (210) is dripped on the area of the second reaction film layer corresponding to the liquid collecting tank (110), the first precipitate is dissolved into a free state under the action of the dissolving solution to obtain a first solution, the first solution penetrates through the second reaction film layer and then enters the liquid collecting tank (110), and the first solution in the liquid collecting tank (110) is dripped to the surface of the first reaction film layer through a first conveying pipe (111);
s400: after the first solution is completely transferred to the first reaction film layer, the first electrode (205) is electrified to carry out electrophoresis on the first solution on the first reaction film layer, and the electrophoresis voltage is adjusted according to the electronegativity strength of the buffer solution, so that when the electrophoresis is in a termination state, the first solution concentrated distribution area is positioned in an area between the two third electrodes (207);
s500: then the two third electrodes (207) are pressed on the first reaction film layer and electrified, the conductivity between the two third electrodes (207) is measured, the concentration of the metabolite of the antitumor drug in the blood is judged according to the conductivity, and the absorption degree of the antitumor drug is detected, so that whether the drug needs to be supplemented or not is judged after the vomit occurs.
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| US3930973A (en) * | 1972-03-10 | 1976-01-06 | Nerenberg Samuel T | Electrophoretic process |
| US5137804A (en) * | 1988-05-10 | 1992-08-11 | E. I. Du Pont De Nemours And Company | Assay device and immunoassay |
| JP2003531362A (en) * | 2000-04-18 | 2003-10-21 | グラディポア・リミテッド | Electrophoretic separation and processing of samples |
| EP1878379B1 (en) * | 2006-07-11 | 2019-10-16 | F. Hoffmann-La Roche AG | Test tape system, in particular for blood sugar analysis |
| GB0618966D0 (en) * | 2006-09-26 | 2006-11-08 | Iti Scotland Ltd | Cartridge system |
| EP1990002B1 (en) * | 2007-05-11 | 2009-12-16 | Roche Diagnostics GmbH | Pricking system, lancet storage system, method for manufacturing such a lancet storage system and method for positioning functional elements arranged on a carrier belt |
| EP2135546B1 (en) * | 2008-06-18 | 2014-09-10 | Roche Diagnostics GmbH | Tape cassette for a portable medical device |
| CN104215757B (en) * | 2010-05-28 | 2018-12-04 | 李金波 | Biological fluid sample quantitative test device and its detection method |
| WO2015184084A2 (en) * | 2014-05-28 | 2015-12-03 | University Of Cincinnati | Sweat monitoring and control of drug delivery |
| CN107719738A (en) * | 2016-08-11 | 2018-02-23 | 深圳安博电子有限公司 | One kind carrier band heat-sealing device |
| EP3306322A1 (en) * | 2016-10-05 | 2018-04-11 | Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen | Method of conducting continuous assays |
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