CN115039742B - Spodoptera frugiperda sex pheromone dual slow-release lure core and preparation method thereof - Google Patents
Spodoptera frugiperda sex pheromone dual slow-release lure core and preparation method thereof Download PDFInfo
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- CN115039742B CN115039742B CN202210573162.9A CN202210573162A CN115039742B CN 115039742 B CN115039742 B CN 115039742B CN 202210573162 A CN202210573162 A CN 202210573162A CN 115039742 B CN115039742 B CN 115039742B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
- A01N25/10—Macromolecular compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P19/00—Pest attractants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M2200/00—Kind of animal
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- A01M2200/012—Flying insects
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Abstract
The invention provides a spodoptera frugiperda sex pheromone dual slow-release lure core and a preparation method thereof, and relates to the technical field of pest control, wherein the spodoptera frugiperda sex pheromone dual slow-release lure core comprises inclusion powder and a high polymer material coated on the outer side of the inclusion powder; the inclusion powder comprises spodoptera littoralis sex pheromone and an inclusion material coated on the outer side of the spodoptera littoralis sex pheromone. According to the spodoptera frugiperda sex pheromone dual slow-release lure, the inclusion material is used for first coating the spodoptera frugiperda sex pheromone, so that the sex pheromone can be released at a relatively stable speed, and then the polymer material is used for second coating, so that the contact of the sex pheromone and the inclusion material with air and sunlight is reduced, the oxidation speed of the sex pheromone is slowed down, and the spodoptera frugiperda sex pheromone dual slow-release lure can obtain a longer duration on the basis of guaranteeing the lure stability.
Description
Technical Field
The invention relates to the technical field of pest control, in particular to a spodoptera frugiperda sex pheromone dual slow-release lure core and a preparation method thereof.
Background
Spodoptera frugiperda (school name: spodoptera frugiperda) commonly known as fall armyworm belongs to the genus spodoptera of the family Lepidoptera, is a globally early-warning migratory agricultural pest for the grain and agriculture organization of the united nations, and is listed in the 'blacklist' of ten plant pests in the world. The larvae of the larvae have preference for gramineous plants such as corn, rice, sugarcane, sorghum and the like, the host plants are more than 300, the fertility is extremely strong, one female moth can spawn 100-200 eggs each time, which is equivalent to 10 times of common plutella xylostella, and can produce more than 1000 eggs per time, only 30 days is needed in summer, once the worms are found in many areas, the whole area can become a heavy disaster area in a short time. The flying force of the adults is particularly strong, and the adults can fly for more than 100 km by wind power in the night, which is not seen in the insect world. Spodoptera frugiperda is primarily in tropical and subtropical areas in america, is invaded africa from america in 2016, then rapidly spreads in 44 countries in saharan, and causes 20% -30% yield reduction of crops such as corn and sugarcane, serious disaster areas even cause harvest failure, the yield reduction of the corn is as high as 830 ten thousand to 2060 ten thousand tons, and the economic loss reaches 23.8-61.9 hundred million dollars. 7 months 2018 is transmitted into Asian areas, and invades India, spearmint and other countries. In 1 month 2019, the method starts to invade Yunnan of China for the first time from southeast Asia, rapidly spreads to Jiangnan and Jianghuai regions, and further spreads to northern corn producing regions. The corn planting area and the total yield of China are inferior to those of the United states, the world is second, and according to the report of national agricultural technology center officials, the pest enters China and becomes a frequently-occurring important pest in North China.
The spodoptera frugiperda has extremely strong drug resistance, and is subjected to the attack of tens of pesticides in tens of countries from America across continents for decades, and has strong resistance to a plurality of pesticides, especially to traditional pesticides such as organophosphorus pesticides, organochlorine pesticides and pyrethroids, when larvae are added, the larvae can often dig into corn leaves to feed, and the pesticide dosage penetrating into the corn leaves during pesticide spraying is generally insufficient to kill pests, so that the actual control effect is very poor. For pests with strong fertility, the control of adult reproduction is more intensive and efficient than the control of next-generation larvae. Sex pheromone is used as a compound for regulating female and male insect attraction, and has strong specificity and attraction. Sex pheromones of a plurality of lepidoptera pests are manually simulated and synthesized, and are made into lures which are placed in a trap for interfering mating of natural populations of the pests so as to achieve the aim of safely and efficiently controlling the pests by regulating reproduction.
Sex pheromone components of spodoptera frugiperda are reported for the first time in 1967, researchers subsequently identify a plurality of components, related lure products have been developed abroad, preliminary research on sex lure products is also carried out after spodoptera frugiperda occurs in China, common spodoptera frugiperda sex lures take rubber heads or plastic tubes as carriers, the adsorption and release capacity of the dosage forms on sex pheromones are limited, effective drug loading capacity cannot be guaranteed during use, and the duration of effect is generally short. Therefore, it is of great importance to find a suitable sustained release dosage form.
Disclosure of Invention
The invention aims to solve the technical problems that: the invention provides a spodoptera frugiperda sex pheromone dual slow release lure core, which aims at solving the problem of shorter duration of sex attractant for spodoptera frugiperda in the prior art, has the effect of dual slow release, can obtain longer duration while guaranteeing the stability of the lure core, and solves the problem of shorter duration of sex attractant in the prior art.
The technical scheme adopted for solving the technical problems is as follows:
a spodoptera frugiperda sex pheromone dual slow-release lure core comprises inclusion powder and a high polymer material coated on the outer side of the inclusion powder; the inclusion powder comprises spodoptera littoralis sex pheromone and an inclusion material coated on the outer side of the spodoptera littoralis sex pheromone.
Optionally, the spodoptera frugiperda sex pheromone comprises cis-9-tetradecene acetate and cis-7-dodecene acetate.
Optionally, the inclusion material comprises beta-cyclodextrin.
Optionally, the polymer material is at least one selected from rosin, shellac and solid paraffin.
The invention also aims to provide a preparation method of the spodoptera frugiperda sex pheromone double slow-release lure core, which comprises the following steps:
s1: dissolving inclusion materials in a proper amount of pure water to obtain inclusion solution;
s2: dripping an organic solution of spodoptera littoralis sex pheromone into the inclusion solution to react at 40-60 ℃ to obtain a reaction mixed solution;
s3: standing the reaction mixed solution at 4 ℃, and performing suction filtration, washing and drying to obtain inclusion powder;
s4: and adding the inclusion powder into a liquid high polymer material, uniformly stirring, cooling and solidifying, and cutting a solid product to obtain the spodoptera frugiperda sex pheromone dual slow-release lure core.
Optionally, in step S1, the mass ratio of the inclusion material to the pure water is 1: (10-30).
Optionally, the mass ratio of the spodoptera frugiperda sex pheromone to the inclusion material is 1: (1-20).
Optionally, the organic solution in step S2 is selected from at least one of methanol solution, ethanol solution, and ethylene glycol solution.
The beneficial effects of the invention are as follows:
according to the spodoptera frugiperda sex pheromone dual slow-release lure, the inclusion material is used for first coating the spodoptera frugiperda sex pheromone, so that the sex pheromone can be released at a relatively stable speed, and then the polymer material is used for second coating, so that the contact of the sex pheromone and the inclusion material with air and sunlight is reduced, the oxidation speed of the sex pheromone is slowed down, and the spodoptera frugiperda sex pheromone dual slow-release lure can obtain a longer duration on the basis of guaranteeing the lure stability.
Detailed Description
The present invention will now be described in further detail. The embodiments described below are exemplary and intended to illustrate the invention and should not be construed as limiting the invention, as all other embodiments, based on which a person of ordinary skill in the art would obtain without inventive faculty, are within the scope of the invention.
In order to solve the problem of shorter duration of sex attractant for spodoptera frugiperda in the prior art, the invention provides a double slow-release attractant core of spodoptera frugiperda sex pheromone, which comprises inclusion powder and a high polymer material coated on the outer side of the inclusion powder; the inclusion powder comprises spodoptera littoralis sex pheromone and an inclusion material coated on the outer side of the spodoptera littoralis sex pheromone.
According to the spodoptera frugiperda sex pheromone dual slow-release lure core, the spodoptera frugiperda sex pheromone is coated for the first time by the inclusion material, so that the sex pheromone can be released at a relatively stable speed, and then is coated for the second time by the high polymer material, the contact of the sex pheromone and the inclusion material with air and sunlight is reduced, the oxidation speed of the sex pheromone is slowed down, and therefore the spodoptera frugiperda sex pheromone dual slow-release lure core has a dual slow-release effect, and a longer lasting period can be obtained on the basis of guaranteeing the stability of the lure core; in addition, the spodoptera frugiperda sex pheromone dual slow-release lure core provided by the invention is convenient to use, is not easily influenced by field environment, and is suitable for preventing, controlling and forecasting spodoptera frugiperda.
In order to ensure the control effect on spodoptera frugiperda, the spodoptera frugiperda sex pheromone of the invention preferably comprises at least one of cis-9-tetradecene acetate, cis-7-dodecene acetate and cis-11-hexadecene acetate; and preferably, the spodoptera littoralis sex pheromone comprises cis-9-tetradecene acetate, cis-7-dodecene acetate and cis-11-hexadecene acetate, wherein the mass ratio of the cis-9-tetradecene acetate, the cis-7-dodecene acetate and the cis-11-hexadecene acetate is 60:1:14; preferably, the spodoptera frugiperda sex pheromone comprises cis-9-tetradecene acetate and cis-7-dodecene acetate, the mass ratio of the cis-9-tetradecene acetate to the cis-7-dodecene acetate is in the range of 60-99:1, and further preferably, the mass ratio of the cis-9-tetradecene acetate to the cis-7-dodecene acetate is in the range of 99:1.
The preferred inclusion material of the present invention is beta-cyclodextrin; the spodoptera frugiperda sex pheromone is coated by adopting the beta-cyclodextrin with stable properties and a cavity structure, so that the contact between the sex pheromone and air and sunlight is reduced, the oxidation speed of the sex pheromone is slowed down, the duration is prolonged, and the stability of the lure core is improved.
In order to effectively reduce the contact of sex pheromone and inclusion materials with air and sunlight, the polymer material is preferably at least one selected from rosin, shellac and solid paraffin, and further preferably is solid paraffin.
Compared with common capillary and rubber head dosage forms, the double slow-release lure core of spodoptera littoralis sex pheromone provided by the invention can ensure the stability of spodoptera littoralis sex pheromone littoralis to the greatest extent, has a better slow-release effect, is not easily influenced by various factors such as strong ultraviolet rays, rainfall, temperature and the like, saves the use cost of manpower, material resources and the like caused by replacing the lure core, and has wider application range and prospect.
The invention also aims to provide a preparation method of the spodoptera frugiperda sex pheromone double slow-release lure core, which comprises the following steps:
s1: dissolving inclusion materials in a proper amount of pure water to obtain inclusion solution;
s2: dripping an organic solution of spodoptera littoralis sex pheromone into the inclusion solution to react at 40-60 ℃ to obtain a reaction mixed solution;
s3: standing the reaction mixed solution at 4 ℃, and performing suction filtration, washing and drying to obtain inclusion powder;
s4: adding the inclusion powder into a liquid polymer material, uniformly stirring, cooling and solidifying, and cutting a solid product to obtain the spodoptera frugiperda sex pheromone dual slow-release lure core.
The liquid polymer material may be melted polymer material or aqueous solution of polymer material, and is determined according to the type of polymer material.
The preparation method of the spodoptera frugiperda sex pheromone double slow release lure provided by the invention has the advantages that the process is simple, the prepared spodoptera frugiperda sex pheromone double slow release lure core is coated for the first time by the inclusion material, so that the spodoptera frugiperda sex pheromone can be released at a relatively stable speed, and then the second time of coating is carried out by the polymer material, the contact of the spodoptera frugiperda sex pheromone and the inclusion material with air and sunlight is reduced, the oxidation speed of the spodoptera frugiperda sex pheromone double slow release lure core has a double slow release effect, and a longer lasting period can be obtained on the basis of guaranteeing the stability of the lure core; in addition, the spodoptera frugiperda sex pheromone dual slow-release lure core provided by the invention is convenient to use, is not easily influenced by field environment, and is suitable for preventing, controlling and forecasting spodoptera frugiperda.
In the preferred step S1, the mass ratio of the inclusion material to the pure water is 1: (10-30), and preferably 1:20; the mass ratio of spodoptera litura sex pheromone to inclusion material is 1: (1-20), and preferably 1:10; the organic solution in the step S2 is at least one selected from methanol solution, ethanol solution and glycol solution; the reaction time in step S2 is preferably 1 to 4 hours, and more preferably 2 hours.
In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of embodiments of the invention will be rendered by reference to specific embodiments thereof which are illustrated in the appended drawings.
Example 1
The embodiment provides a spodoptera frugiperda sex pheromone dual slow-release lure core, which is prepared by the following steps:
s1: beta-cyclodextrin (10 g) is weighed into a 500mL round bottom beaker, 200mL of purified water is added, and the mixture is completely stirred and dissolved in water bath at 50 ℃ to obtain inclusion solution;
s2: dripping 5mL 200mg/mL spodoptera littoralis sex pheromone ethanol solution into the inclusion solution, and continuously stirring at constant temperature for 2 hours to obtain a reaction mixed solution; wherein the spodoptera littoralis sex pheromone comprises cis-9-tetradecene acetate and cis-7-dodecene acetate, and the mass ratio of the cis-9-tetradecene acetate to the cis-7-dodecene acetate is 99:1.
S3: stopping heating, cooling the system to room temperature, placing in a refrigerator at 4 ℃ for refrigeration for 10 hours, and then filtering while the system is cold, and washing with a small amount of petroleum ether; collecting a filter cake, and baking at 60 ℃ for 20 minutes to obtain inclusion powder;
s4: weighing solid paraffin (20 g) in a 100mL round-bottom flask, heating until the solid paraffin is melted, adding inclusion powder (5 g), stirring uniformly, pouring into a culture dish, and cooling and solidifying; cutting the solidified and formed solid into small blocks with the mass of 0.25g to obtain the spodoptera frugiperda sex pheromone double slow-release lure core.
Example 2
The difference between this example and example 1 is that the weight ratio of spodoptera frugiperda sex pheromone to beta-cyclodextrin is 1:20.
Example 3
The difference between this example and example 1 is that the weight ratio of spodoptera frugiperda sex pheromone to beta-cyclodextrin is 1:5.
Example 4
The difference between this example and example 1 is that the weight ratio of spodoptera frugiperda sex pheromone to beta-cyclodextrin is 1:1.
Example 5
This example differs from example 1 in that the weight ratio of beta-cyclodextrin to pure water is 1:30.
Example 6
This example differs from example 1 in that the weight ratio of beta-cyclodextrin to pure water is 1:10.
Example 7
The difference between this embodiment and embodiment 1 is that the organic solution in step S2 is a methanol solution.
Example 8
The difference between this embodiment and embodiment 1 is that the organic solution in step S2 is an ethylene glycol solution.
Example 9
The difference between this example and example 1 is that the heating temperature in step S1 is 60 ℃.
Example 10
The difference between this example and example 1 is that the heating temperature in step S1 is 40 ℃.
Example 11
The difference between this example and example 1 is that the stirring time in step S2 was 4 hours.
Example 12
The difference between this example and example 1 is that the stirring time in step S2 was 3 hours.
Example 13
The difference between this example and example 1 is that the stirring time in step S2 is 1 hour.
Example 14
The difference between this embodiment and embodiment 1 is that step S4 is: weighing lac (20 g) in a 100mL round bottom flask, heating until the lac is melted, adding inclusion powder (5 g), stirring uniformly, pouring into a culture dish, and cooling and solidifying; cutting the solidified and formed solid into small blocks with the mass of 0.25g to obtain the spodoptera frugiperda sex pheromone double slow-release lure core.
Example 15
The difference between this embodiment and embodiment 1 is that step S4 is: weighing rosin (20 g) in a 100mL round-bottom flask, heating until the rosin is melted, adding inclusion powder (5 g), stirring uniformly, pouring into a culture dish, and cooling and solidifying; cutting the solidified and formed solid into small blocks with the mass of 0.25g to obtain the spodoptera frugiperda sex pheromone double slow-release lure core.
Comparative example 1
The difference between this comparative example and example 1 is that step S4 is: starch (20 g) is weighed into a 100mL round bottom flask, stirred at the water adding temperature, inclusion powder (5 g) is added, stirred uniformly, poured into a culture dish for airing, divided into 0.25g each part, and filled into a gauze bag.
Comparative example 2
The difference between this comparative example and example 1 is that step S4 is: diatomite (20 g) is weighed into a 100mL round bottom flask, stirred at a water temperature, added with inclusion powder (5 g), stirred uniformly, poured into a culture dish for airing, divided into 0.25g each part, and filled into a gauze bag.
Comparative example 3
Mixing cis-9-tetradecene acetate and cis-7-dodecene acetate at a ratio of 99:1, adding corn oil to obtain spodoptera frugiperda sex pheromone solution, filling the solution into capillary, cutting into 8cm long cores, and preserving at low temperature for use.
Comparative example 4
Mixing cis-9-tetradecene acetate and cis-7-dodecene acetate uniformly according to the ratio of 99:1, adding n-hexane to prepare spodoptera frugiperda sex pheromone solution, quantitatively adding the solution into a concave cup of a silicon rubber plug, and after the n-hexane is volatilized, preserving the prepared spodoptera frugiperda sex lure at a low temperature for later use.
Comparative example 5
Provides a spodoptera frugiperda sex pheromone slow-release lure core, which comprises the following preparation method:
s1: beta-cyclodextrin (10 g) is weighed into a 500mL round bottom beaker, 200mL of purified water is added, and the mixture is completely stirred and dissolved in water bath at 50 ℃ to obtain inclusion solution;
s2: dripping 5mL 200mg/mL spodoptera littoralis sex pheromone ethanol solution into the inclusion solution, and continuously stirring at constant temperature for 3 hours to obtain a reaction mixed solution;
s3: stopping heating, cooling the system to room temperature, placing in a refrigerator at 4 ℃ for refrigeration for 10 hours, and then filtering while the system is cold, and washing with a small amount of petroleum ether; and (5) collecting a filter cake, and baking at 60 ℃ for 20 minutes to obtain the spodoptera frugiperda sex pheromone slow-release lure core.
The lures prepared in each example and comparative example were tested.
1. Examples determination of inclusion Rate of Spodoptera frugiperda sex pheromone in inclusion powder
The inclusion rate of spodoptera littoralis sex pheromone is taken as an investigation index, and the calculation method comprises the following formula:
inclusion ratio (%) = [ amount of pheromone to be included/amount of pheromone to be fed ] ×100%
Collecting filtrate generated during preparation of inclusion powder, extracting with n-hexane, drying with desiccant, concentrating under reduced pressure, transferring into 10mL volumetric flask, adding 1mL 1mg/mL octadecane, metering n-hexane to scale, and shaking; detecting and calculating the amount of the included pheromone by gas chromatography; the inclusion rate results are shown in Table 1:
table 1 results of inclusion ratio of beta-cyclodextrin/spodoptera frugiperda sex pheromone inclusion compound
2. Stability test of spodoptera frugiperda sex pheromone double slow-release lure core
The lures obtained in example 1, example 14, example 15 and comparative examples 1 to 5 were placed in a natural environment for 90 days, weighed every 30 days, and the content of the remaining active ingredients was calculated by a weight loss method, and the result is shown in table 2, and the calculation formula of the content X is as follows:
wherein m is the weight of the active ingredient in the sample at the beginning of release of m 1 For the weight of the sample at different time points, m 0 To the weight of the sample at the beginning of release.
TABLE 2 content of residual active ingredients of different lures placed in Natural Environment for 90 days
From the data, compared with the comparative examples, the double slow-release lure core of spodoptera frugiperda sex pheromone coated by paraffin, lac and rosin has the advantages that the release of the active ingredients is slower and more uniform, so that the double slow-release lure core provided by the invention has better stability.
3. Double slow release core-inducing field application effect of spodoptera litura sex pheromone
The field trapping test is carried out in the village of the Committee under the gate dam of the mountain bridge, the Zhenhan, the Hu village of the Wu-go area of the Changzhou province of Jiangsu province. Test groups were 3, namely, the lures obtained in example 1, example 14 and example 15, and comparative example 5, wherein the lures were coated with starch in comparative example 1, diatomite in comparative example 2, capillary type lures in comparative example 3, rubber head type lures in comparative example 4, and the sex pheromones were coated with inclusion material only in comparative example 5 without secondary coating with polymer material. Each lure core is matched with the spodoptera frugiperda trapper for use, each lure core is repeated for 3 times, so that the trapper is ensured to be covered uniformly as much as possible, and errors caused by uneven release at different positions are eliminated; the number of trapped insects was counted for 3 months, and the trapping results are shown in Table 3.
TABLE 3 results of different lure trapping experiments
As shown in Table 3, the double lure prepared by dispersing the beta-cyclodextrin/spodoptera frugiperda sex pheromone inclusion compound in paraffin, lac and rosin has good trapping effect on spodoptera frugiperda, and the duration of the double lure can reach more than three months, but the lure trapping effect of the early-stage solid paraffin inclusion is superior to that of the lure of the lac and rosin inclusion, so that the lure of paraffin inclusion has more application value in comprehensive consideration.
The lure coated by the starch and the diatomite has a certain slow release effect, but the slow release effect is not obvious after three months.
The core coated with beta-cyclodextrin alone in comparative example 5 had an increased duration of time over the commercially available capillary core and rubber head core, but was much less than the core coated with paraffin, shellac and rosin.
From the data, the scheme provided by the invention ensures that the prepared spodoptera frugiperda sex pheromone dual slow release lure core can obtain a longer duration on the basis of ensuring the lure core stability through the two coating processes of the inclusion material and the high polymer material.
With the above-described preferred embodiments according to the present invention as an illustration, the above-described descriptions can be used by persons skilled in the relevant art to make various changes and modifications without departing from the scope of the technical idea of the present invention. The technical scope of the present invention is not limited to the description, but must be determined according to the scope of claims.
Claims (4)
1. The spodoptera frugiperda sex pheromone double slow-release lure core is characterized by comprising inclusion powder and a polymer material coated on the outer side of the inclusion powder; the inclusion powder comprises spodoptera littoralis sex pheromone and an inclusion material coated on the outer side of the spodoptera littoralis sex pheromone; the high polymer material is at least one selected from rosin, shellac and solid paraffin; the inclusion material comprises beta-cyclodextrin; the mass ratio of the spodoptera littoralis sex pheromone to the inclusion material is 1: (1-20); the mass ratio of the polymer material to the inclusion powder is 4:1;
the spodoptera frugiperda sex pheromone double slow-release lure core is prepared according to the following method:
s1: dissolving inclusion materials in a proper amount of pure water to obtain inclusion solution;
s2: dripping an organic solution of spodoptera littoralis sex pheromone into the inclusion solution to react at 40-60 ℃ to obtain a reaction mixed solution;
s3: standing the reaction mixed solution at 4 ℃, and performing suction filtration, washing and drying to obtain inclusion powder;
s4: and adding the inclusion powder into a liquid high polymer material, uniformly stirring, cooling and solidifying, and cutting a solid product to obtain the spodoptera frugiperda sex pheromone dual slow-release lure core.
2. A dual sustained release lure for spodoptera littoralis sex pheromone as claimed in claim 1 wherein said spodoptera littoralis sex pheromone includes cis-9-tetradecene acetate and cis-7-dodecene acetate.
3. The spodoptera frugiperda sex pheromone dual slow release lure according to claim 1, wherein the mass ratio of the inclusion material to the pure water in the step S1 is 1: (10-30).
4. The spodoptera frugiperda sex pheromone dual slow release lure according to claim 1, wherein the organic solution in step S2 is at least one selected from the group consisting of methanol solution, ethanol solution and ethylene glycol solution.
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Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5650160A (en) * | 1992-06-18 | 1997-07-22 | Ncsr "Demokritos" | Inclusion complexes of cyclodextrin and their use in slow release formulations for attracting insects |
| CN101617652A (en) * | 2008-07-02 | 2010-01-06 | 宁波纽康生物技术有限公司 | Striped rice borer sex pheromone composition and inducement core thereof |
| CN101703043A (en) * | 2009-11-10 | 2010-05-12 | 杜进平 | Preparation method of sexual attraction slow-release formulation for fruit flies |
| JP2011046739A (en) * | 2010-11-22 | 2011-03-10 | Shin-Etsu Chemical Co Ltd | Sustained release preparation containing two or more sex pheromone substances and controlling method |
| CN102369925A (en) * | 2010-08-17 | 2012-03-14 | 宁波纽康生物技术有限公司 | Sex pheromone composition for luring citrus leaf miner and luring core thereof |
| CN102657156A (en) * | 2012-04-13 | 2012-09-12 | 西南林业大学 | Preparation of beta-cyclodextrin sustained-release microcapsule of forestry insect repellant (E)-2-hexenal and determination method |
| CN103828808A (en) * | 2014-03-03 | 2014-06-04 | 山东省花生研究所 | Pasty Holotrichia parallela sex attractant sustained-release preparation and preparation method thereof |
| CN104186469A (en) * | 2014-09-12 | 2014-12-10 | 陕西理工学院 | Sex attractant and attraction core for attracting chilo suppressalis |
| WO2016044976A1 (en) * | 2014-09-22 | 2016-03-31 | 江苏大学 | Double-coating cyclosporine a sustained-release pellet preparation and preparation method therefor |
| CN108651451A (en) * | 2018-05-03 | 2018-10-16 | 贵州省生物技术研究所 | A kind of sex pheromones of Spodoptera litura Fabricius lure slow-released carrier and preparation method thereof |
| CN108883125A (en) * | 2016-02-04 | 2018-11-23 | 西泽普研究与发展有限责任公司 | The cyclodextrin inclusion compound medium of controlled release and stratiform |
| CN109874789A (en) * | 2019-03-01 | 2019-06-14 | 常州大学 | A method for slowly releasing pest sex pheromone using slow-release agent and carrier double slow-release technology |
| CN113940324A (en) * | 2020-07-17 | 2022-01-18 | 北京依科曼生物技术股份有限公司 | Preparation method of spodoptera frugiperda sex attractant core |
| CN114466591A (en) * | 2019-06-04 | 2022-05-10 | 普罗维维公司 | Non-natural insect pheromone blend compositions |
| CN114451407A (en) * | 2021-12-03 | 2022-05-10 | 福建省农业科学院植物保护研究所 | A kind of Spodoptera frugiperda sex attractant and preparation method and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5777478B2 (en) * | 2011-10-12 | 2015-09-09 | 信越化学工業株式会社 | Water-dispersible pheromone sustained release formulation |
-
2022
- 2022-05-24 CN CN202210573162.9A patent/CN115039742B/en active Active
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5650160A (en) * | 1992-06-18 | 1997-07-22 | Ncsr "Demokritos" | Inclusion complexes of cyclodextrin and their use in slow release formulations for attracting insects |
| CN101617652A (en) * | 2008-07-02 | 2010-01-06 | 宁波纽康生物技术有限公司 | Striped rice borer sex pheromone composition and inducement core thereof |
| CN101703043A (en) * | 2009-11-10 | 2010-05-12 | 杜进平 | Preparation method of sexual attraction slow-release formulation for fruit flies |
| CN102369925A (en) * | 2010-08-17 | 2012-03-14 | 宁波纽康生物技术有限公司 | Sex pheromone composition for luring citrus leaf miner and luring core thereof |
| JP2011046739A (en) * | 2010-11-22 | 2011-03-10 | Shin-Etsu Chemical Co Ltd | Sustained release preparation containing two or more sex pheromone substances and controlling method |
| CN102657156A (en) * | 2012-04-13 | 2012-09-12 | 西南林业大学 | Preparation of beta-cyclodextrin sustained-release microcapsule of forestry insect repellant (E)-2-hexenal and determination method |
| CN103828808A (en) * | 2014-03-03 | 2014-06-04 | 山东省花生研究所 | Pasty Holotrichia parallela sex attractant sustained-release preparation and preparation method thereof |
| CN104186469A (en) * | 2014-09-12 | 2014-12-10 | 陕西理工学院 | Sex attractant and attraction core for attracting chilo suppressalis |
| WO2016044976A1 (en) * | 2014-09-22 | 2016-03-31 | 江苏大学 | Double-coating cyclosporine a sustained-release pellet preparation and preparation method therefor |
| CN108883125A (en) * | 2016-02-04 | 2018-11-23 | 西泽普研究与发展有限责任公司 | The cyclodextrin inclusion compound medium of controlled release and stratiform |
| CN108651451A (en) * | 2018-05-03 | 2018-10-16 | 贵州省生物技术研究所 | A kind of sex pheromones of Spodoptera litura Fabricius lure slow-released carrier and preparation method thereof |
| CN109874789A (en) * | 2019-03-01 | 2019-06-14 | 常州大学 | A method for slowly releasing pest sex pheromone using slow-release agent and carrier double slow-release technology |
| CN114466591A (en) * | 2019-06-04 | 2022-05-10 | 普罗维维公司 | Non-natural insect pheromone blend compositions |
| CN113940324A (en) * | 2020-07-17 | 2022-01-18 | 北京依科曼生物技术股份有限公司 | Preparation method of spodoptera frugiperda sex attractant core |
| CN114451407A (en) * | 2021-12-03 | 2022-05-10 | 福建省农业科学院植物保护研究所 | A kind of Spodoptera frugiperda sex attractant and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 草地贪夜蛾不同配方性诱剂诱捕试验;沈嘉彬等;《中国植保导刊》;20191225(第12期);全文 * |
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