CN115032133B - Endothelial cell monolayer permeability detection experimental device - Google Patents

Abstract

The invention relates to a detection experimental device, in particular to an endothelial cell monolayer permeability detection experimental device. The invention aims to provide an endothelial cell monolayer permeability test device which can be automatically operated and kill residual bacteria. The invention provides an endothelial cell monolayer permeability detection experimental device which comprises a base, an isolation cover, a movable door and a conductivity tester, wherein the top of the base is connected with the isolation cover, the front part of the isolation cover is rotatably connected with the movable door, the conductivity tester is used for experimental detection, the device also comprises a display and the like, and the upper part of the front side of the isolation cover is connected with the display. According to the invention, the first motor and the second motor are started, the electric liquid suction device and the electric sucking disc can be controlled to rotate and move, then the electric liquid suction device and the electric sucking disc are started, so that the liquid medicine can be automatically fed, and the transwell chamber is automatically placed in the glass cup, thereby facilitating detection of people in a closed space.

Description

An experimental device for detecting the permeability of endothelial cell monolayer

Technical Field

The invention relates to a detection experimental device, in particular to an endothelial cell monolayer permeability detection experimental device.

Background technique

At present, it is usually difficult to measure the permeability of human vascular walls, and due to the influence of multiple complex factors in the body, it is difficult to observe the impact of a single factor on vascular wall permeability. For this reason, the in vitro determination of monolayer endothelial permeability has become the main means of studying the permeability function of the endothelial monolayer. Compared with the albumin filtration determination method, the conductivity determination method of monolayer endothelial permeability is simpler to operate, and the technical sensitivity and sensitivity are significantly improved.

However, during the experiment, the conductivity measurement method also requires manual operation step by step. Since it is difficult to achieve a good isolation effect during manual operation, the endothelial cells are easily contaminated by bacteria. Moreover, before the experiment, people need to open the sterile room and put in various experimental equipment, which makes it easy for outside air to enter the sterile room, thus affecting the test results.

Therefore, in view of the above-mentioned deficiencies, an endothelial cell monolayer permeability detection experimental device which can be automatically operated and can kill residual bacteria is provided.

Summary of the invention

In order to overcome the shortcomings that people need to manually operate the conductivity measurement method for monolayer endothelial permeability, and when various experimental equipment are placed, bacteria can easily enter the sterile room and stay inside, thereby affecting the test results, the purpose of the present invention is to provide an endothelial cell monolayer permeability detection experimental device that can be automatically operated and can kill residual bacteria.

The present invention is achieved through the following technical approaches:

An endothelial cell monolayer permeability detection experimental device includes a base, an isolation cover, a movable door and a conductivity tester. The top of the base is connected to the isolation cover, and the front of the isolation cover is rotatably connected to the movable door. The conductivity tester is used for experimental detection, and also includes a display, a first placement rack, a second placement rack, a control mechanism, a testing mechanism, a sterilization mechanism and an isolation mechanism. The upper front side of the isolation cover is connected to the display, the front right side of the top of the base is connected to the first placement rack, and the rear right side of the top of the base is connected to the second placement rack. The first placement rack and the second placement rack are both located inside the isolation cover. The upper side of the base is provided with a control mechanism for automatic material preparation, and the middle of the rear side of the top of the base is provided with a control mechanism for making the conductivity tester A movable testing mechanism, a conductivity tester is installed on the testing mechanism, the conductivity tester is electrically connected to a display, a sterilization mechanism is provided on the upper inner side of the isolation cover for sterilization, an isolation mechanism is provided between the first placement rack and the second placement rack for blocking the experimental instrument, the experimental instrument is placed on the first placement rack and the second placement rack, the isolation cover and the movable door are used to form a closed space, the experimental instrument is then blocked by the isolation mechanism, the closed space is then sterilized by the sterilization mechanism, the experimental instrument and the liquid medicine are prepared by the control mechanism, and then the conductivity tester is driven to move by the testing mechanism to start detection, and the display is used to display the detection data.

In one embodiment, the control mechanism includes a first motor, a guide frame, a second motor, a first screw rod, a first movable plate, an electric liquid suction device and an electric suction cup. The first motor is connected to the right side of the middle upper side of the base, and the guide frame is rotatably connected to the right side of the middle upper side of the base. The bottom of the guide frame is connected to the output shaft of the first motor, and the top of the guide frame is connected to the second motor. The guide frame is rotatably connected to the first screw rod, and the top of the first screw rod is connected to the output shaft of the second motor. The first movable plate is slidably connected to the guide frame, and the first movable plate is threadedly connected to the first screw rod. The electric liquid suction device is connected to the left side of the first movable plate, and the electric suction cup is connected to the right side of the first movable plate. The electric liquid suction device and the electric suction cup are driven to rotate and move by turning on the first motor and the second motor, and then the electric liquid suction device and the electric suction cup are turned on to realize automatic material preparation.

In one embodiment, the testing mechanism includes a support frame, a third motor, a second screw rod and a second movable plate. The support frame is connected to the middle part of the rear side of the top of the base, the third motor is connected to the top of the support frame, the second screw rod is rotatably connected inside the support frame, the top of the second screw rod is connected to the output shaft of the third motor, the second movable plate is threadedly connected to the second screw rod, the second movable plate is slidably connected to the support frame, and the front part of the second movable plate is connected to the conductivity tester. When the third motor is turned on, the second screw rod is driven to rotate, so that the second movable plate and the conductivity tester move.

In one embodiment, the sterilization mechanism includes an annular rack, a slider, a fourth motor, a first gear and a hydrogen peroxide sterilizer. The upper inner part of the isolation cover is connected to the annular rack, the slider is slidably connected to the inner side of the annular rack, the upper part of the slider is connected to the fourth motor, the output shaft of the fourth motor is connected to the first gear, the first gear is meshed with the annular rack, and the front side of the slider is connected to the hydrogen peroxide sterilizer. When the fourth motor is turned on, the first gear is driven to rotate, causing the slider and the hydrogen peroxide sterilizer to move, and then the hydrogen peroxide sterilizer is turned on to achieve sterilization of the inside of the isolation cover.

In one embodiment, the isolation mechanism includes a rotating rod, a baffle, an elastic member, a second gear and a toothed plate. The first placement rack and the second placement rack are both rotatably connected to the outer sides of the tops of the rotating rods, and the baffles are connected to the lower sides of the rotating rods. An elastic member is connected between the front baffle and the first placement rack, and an elastic member is also connected between the rear baffle and the second placement rack. The upper sides of the rotating rods are connected to the second gears through a one-way clutch, and the upper left side and the top right side of the guide frame are connected to the toothed plate. When the toothed plate rotates, it will contact the second gear, and the experimental instrument will be placed by pushing the baffle to rotate. The elastic member is used to reset the baffle so that the baffle covers the experimental instrument.

In one of the embodiments, a cleaning mechanism for wiping the isolation cover is also included. The cleaning mechanism includes a support rod and wiping cotton. The annular slide groove is located inside the isolation cover. The lower rear side of the slider is connected to the support rod. The rear side of the support rod is connected to the wiping cotton. The wiping cotton is in contact with the inner wall of the isolation cover. The support rod and the wiping cotton move to make the wiping cotton wipe the inner wall of the isolation cover.

In one of the embodiments, a blocking mechanism for protection is also included, and the blocking mechanism includes a protective shell and a rubber sleeve. The upper inner part of the annular rack is connected to the protective shell, the outer side of the protective shell is connected to the isolation cover, and the guide frame is connected to the rubber sleeve. The left and right sides of the first movable plate pass through the rubber sleeve. The fourth motor and the first screw rod are protected by the protective shell and the rubber sleeve.

In one embodiment, the isolation cover is made of transparent material.

In one embodiment, the elastic member is a torsion spring.

In one embodiment, an annular groove is provided on the outer side of the top of the base, and the support rod slides in the annular groove.

The present invention provides an endothelial cell monolayer permeability detection experimental device, which has the following advantages:

1. After placing the glass cup, the liquid medicine tube containing liquid medicine and the transwell chamber, the present invention can sterilize the inside of the isolation cover by turning on the hydrogen peroxide sterilizer, thereby preventing the bacteria remaining in the isolation cover from affecting the test results.

2. The present invention can control the electric pipette and the electric suction cup to rotate and move by turning on the first motor and the second motor. Then, by turning on the electric pipette and the electric suction cup, the drug solution can be automatically loaded and the transwell chamber can be automatically placed in the glass cup, thereby facilitating people to conduct detection in a closed space.

3. Under the action of the wiping cotton, the wiping cotton can move in a circular manner, so that the wiping cotton can wipe the inner wall of the isolation cover, thereby making it convenient for people to observe the internal situation through the isolation cover.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG1 is a schematic diagram of the three-dimensional structure of the present invention.

FIG. 2 is a schematic diagram of a partial three-dimensional structure of the present invention.

FIG. 3 is a schematic diagram of the three-dimensional structure of the testing mechanism of the present invention.

FIG. 4 is a schematic diagram of a partial three-dimensional structure of the testing mechanism of the present invention.

FIG. 5 is a schematic diagram of the three-dimensional structure of the control mechanism of the present invention.

FIG. 6 is a schematic diagram of a partial three-dimensional structure of the control mechanism of the present invention.

FIG. 7 is a schematic diagram of the three-dimensional structure of the isolation mechanism of the present invention.

FIG. 8 is a schematic diagram of a partial three-dimensional structure of the isolation mechanism of the present invention.

FIG. 9 is a schematic diagram of the three-dimensional structure of the sterilization mechanism of the present invention.

FIG. 10 is a schematic diagram of the three-dimensional structure of the cleaning mechanism of the present invention.

FIG. 11 is a schematic diagram of the three-dimensional structure of the blocking mechanism of the present invention.

The markings in the figure are: 1-base, 2-isolation cover, 3-movable door, 4-display, 5-first placement rack, 6-second placement rack, 7-conductivity tester, 8-control mechanism, 81-first motor, 82-guide frame, 83-second motor, 84-first screw rod, 85-first movable plate, 86-electric liquid pipette, 87-electric suction cup, 9-testing mechanism, 91-support frame, 92-third motor, 93-second screw rod, 94-second movable plate, 10-sterilization mechanism, 101-annular rack, 102 -slider, 103-fourth motor, 104-first gear, 105-hydrogen peroxide sterilizer, 11-isolating mechanism, 1101-rotating rod, 1102-baffle, 1103-elastic member, 1104-second gear, 1105-tooth plate, 12-cleaning mechanism, 1201-annular slide, 1202-support rod, 1203-wiping cotton, 13-blocking mechanism, 1301-protective shell, 1302-rubber sleeve, 14-glass cup, 15-medicine liquid tube, 16-transwell chamber.

Detailed ways

The present invention is further described below with reference to the accompanying drawings, and embodiments of the present invention are given with reference to the accompanying drawings.

Example 1

An endothelial cell monolayer permeability detection experimental device includes a base 1, an isolation cover 2, a movable door 3, a display 4, a first placement rack 5, a second placement rack 6, a conductivity tester 7, a control mechanism 8, a testing mechanism 9, a sterilization mechanism 10 and an isolation mechanism 11. Referring to Figures 1 to 9, the top outer side of the base 1 is connected to the isolation cover 2, the isolation cover 2 is made of a transparent material, the lower front side of the isolation cover 2 is rotatably connected to the movable door 3, and the upper front side of the isolation cover 2 is connected to the display 4. By opening the display 4, people can conveniently view the detection data. A first placement rack 5 is connected to the right portion of the top front side of the base 1, and a second placement rack 6 is connected to the right portion of the top rear side of the base 1. Both the first placement rack 5 and the second placement rack 6 are located inside the isolation cover 2. A control mechanism 8 is provided on the upper side of the base 1, and a testing mechanism 9 is provided in the middle portion of the top rear side of the base 1. A conductivity tester 7 is connected to the testing mechanism 9. The conductivity tester 7 is turned on to start the test. The conductivity tester 7 is electrically connected to the display 4. A sterilization mechanism 10 is provided on the upper portion of the inner side of the isolation cover 2, and an isolation mechanism 11 is provided between the first placement rack 5 and the second placement rack 6.

The control mechanism 8 includes a first motor 81, a guide frame 82, a second motor 83, a first screw rod 84, a first movable plate 85, an electric liquid suction device 86 and an electric suction cup 87. Referring to Figures 5 and 6, the first motor 81 is bolted to the right side of the middle upper side of the base 1, and the guide frame 82 is rotatably connected to the right side of the middle upper side of the base 1. The bottom of the guide frame 82 is connected to the output shaft of the first motor 81, and the top of the guide frame 82 is connected to the second motor 83. The first movable plate 85 is slidably connected to the guide frame 82, and the first movable plate 85 is threadedly connected to the first screw rod 84. The electric liquid suction device 86 is connected to the left side of the first movable plate 85, and the electric suction cup 87 is connected to the right side of the first movable plate 85. The first motor 81, the second motor 83, the electric liquid suction device 86 and the electric suction cup 87 are turned on to automatically prepare materials.

The testing mechanism 9 includes a support frame 91, a third motor 92, a second screw rod 93 and a second movable plate 94. Referring to Figures 3 and 4, the support frame 91 is connected to the middle of the top rear side of the base 1, and the third motor 92 is bolted to the top of the support frame 91. When the third motor 92 is turned on, the conductivity tester 7 can be moved. The second screw rod 93 is rotatably connected inside the support frame 91, and the top of the second screw rod 93 is connected to the output shaft of the third motor 92. The second movable plate 94 is threadedly connected to the second screw rod 93, and the second movable plate 94 is slidably connected to the support frame 91. The front of the second movable plate 94 is connected to the conductivity tester 7.

The sterilization mechanism 10 includes an annular rack 101, a slider 102, a fourth motor 103, a first gear 104 and a hydrogen peroxide sterilizer 105. As shown in Figure 9, the annular rack 101 is connected to the upper inner part of the isolation cover 2, the slider 102 is slidably connected to the inner side of the annular rack 101, the upper part of the slider 102 is connected to the fourth motor 103, the output shaft of the fourth motor 103 is connected to the first gear 104, the first gear 104 is meshed with the annular rack 101, and the front side of the slider 102 is connected to the hydrogen peroxide sterilizer 105. By turning on the hydrogen peroxide sterilizer 105, the inside of the isolation cover 2 can be sterilized.

The isolation mechanism 11 includes a rotating rod 1101, a baffle 1102, an elastic member 1103, a second gear 1104 and a tooth plate 1105. Referring to FIG. 7 and FIG. 8, the top outer sides of the first placement rack 5 and the second placement rack 6 are both rotatably connected to the rotating rod 1101, and the lower sides of the two rotating rods 1101 are both connected to the baffle 1102. The baffle 1102 is reversed to the top of the drug liquid tube 15 and the transwell chamber 16, and the drug liquid tube 15 and the transwell chamber 16 can be ll small chamber 16 is blocked, an elastic member 1103 is connected between the front baffle 1102 and the first placement rack 5, and the elastic member 1103 is a torsion spring. An elastic member 1103 is also connected between the rear baffle 1102 and the second placement rack 6. The upper sides of the two rotating rods 1101 are connected to the second gear 1104 through a one-way clutch, and the upper left part and the top right side of the guide frame 82 are connected to a toothed plate 1105, which will contact the second gear 1104 when rotating.

When people need to use the endothelial cell monolayer permeability detection experimental device, first put the polycarbonate membrane into the transwell chamber 16, then pull the movable door 3 to rotate and open it, then put the glass cup 14 on the lower part of the support frame 91, so that the glass cup 14 is directly under the electric pipette 86, then push the baffle 1102 to rotate, the elastic member 1103 is deformed, and then put the drug solution tube 15 containing the drug solution on the second placement rack 6, then put the transwell chamber 16 on the first placement rack 5, and then release the baffle. 1102, the elastic member 1103 returns to its original state, and the elastic member 1103 drives the baffle 1102 to reverse and reset, so that the baffle 1102 covers the drug liquid tube 15 and the transwell chamber 16, and then pushes the movable door 3 to reverse and close, and then the fourth motor 103 is turned on, so that the output shaft of the fourth motor 103 drives the first gear 104 to rotate, and under the action of the annular rack 101, the first gear 104, the fourth motor 103 and the slider 102 move in an annular manner, and then the hydrogen peroxide sterilizer 105 is turned on to sterilize the hydrogen peroxide. The sterilizer 105 sterilizes the inside of the isolation cover 2. When the inside of the isolation cover 2 is sterilized, the hydrogen peroxide sterilizer 105 and the fourth motor 103 are turned off. After a period of time, the first motor 81 is turned on again to control the output shaft of the first motor 81 to rotate forward, thereby driving the guide frame 82, the tooth plate 1105, the first movable plate 85, the electric liquid suction device 86 and the electric suction cup 87 to rotate forward, so that the electric liquid suction device 86 rotates to the top of the liquid medicine tube 15. When the rear tooth plate 1105 contacts the rear second gear 1104, the rear tooth plate 1105 contacts the rear second gear 1104. The second gear 1104 at the rear side is driven to rotate by the second motor 81, thereby driving the rotating rod 1101 and the baffle 1102 at the rear side to rotate, and the elastic member 1103 is deformed, so that the baffle 1102 at the rear side no longer blocks the liquid medicine tube 15, and then the output shaft of the first motor 81 is controlled to stop rotating forward, and then the second motor 83 is turned on, and the output shaft of the second motor 83 is controlled to rotate forward, thereby driving the first screw rod 84 to rotate forward, so that the first movable plate 85, the electric liquid suction device 86 and the electric suction cup 87 move downward, so that the electric liquid suction device 86 is inserted into the liquid medicine tube 15. 5, then the output shaft of the second motor 83 is controlled to stop forward rotation, and then the electric liquid suction device 86 is controlled to be turned on, so that the electric liquid suction device 86 sucks up the liquid in the liquid medicine tube 15, and then the electric liquid suction device 86 is closed, and then the output shaft of the second motor 83 is controlled to reverse, thereby driving the first screw rod 84 to rotate forward, so that the first movable plate 85, the electric liquid suction device 86 and the electric suction cup 87 move upward and reset, so that the electric liquid suction device 86 leaves the liquid medicine in the liquid medicine tube 15, and then the output shaft of the second motor 83 is controlled to stop reverse rotation, and then the first The output shaft of the motor 81 is reversed and reset, thereby driving the guide frame 82, the tooth plate 1105, the first movable plate 85, the electric liquid suction device 86 and the electric suction cup 87 to reverse and reset, so that the electric liquid suction device 86 is reversed to the top of the glass cup 14, so that the rear tooth plate 1105 drives the rear second gear 1104 to reverse and reset, thereby driving the rear rotating rod 1101 and the rear baffle 1102 to reverse and reset, and the elastic member 1103 returns to its original state, so that the rear baffle 1102 blocks the liquid medicine tube 15 again. When the rear tooth plate 1105 When the electric suction device 86 is separated from the second gear 1104 at the rear side, the second gear 1104 at the rear side, the rotating rod 1101 at the rear side and the baffle 1102 at the rear side stop reversing, and then the output shaft of the first motor 81 is controlled to stop reversing, and the output shaft of the second motor 83 is controlled to rotate forward again, so that the electric suction device 86 and the electric suction cup 87 move downward, so that the electric suction device 86 enters the glass 14, and then the output shaft of the second motor 83 is controlled to stop rotating forward, and the electric suction device 86 is controlled to start again, so that the electric suction device 86 sprays the liquid medicine into the glass 14. 4, then close the electric pipette 86, control the output shaft of the second motor 83 to reverse again, so that the electric pipette 86 and the electric suction cup 87 move upward and reset, thereby completing one time of liquid medicine loading, and then control the output shaft of the first motor 81 to rotate forward again, thereby driving the guide frame 82, the tooth plate 1105, the first movable plate 85, the electric pipette 86 and the electric suction cup 87 to rotate forward, so that the electric suction cup 87 rotates to the top of the transwell chamber 16, when the tooth plate 1105 on the front side contacts the second gear 1104 on the front side When the first motor 81 is moved to the left, the second gear 1104 is driven to rotate by the toothed plate 1105, thereby driving the rotating rod 1101 and the baffle 1102 to rotate, and the elastic member 1103 is deformed, so that the baffle 1102 no longer blocks the transwell chamber 16, and then the output shaft of the first motor 81 is controlled to stop rotating forward, and then the output shaft of the second motor 83 is controlled to rotate forward, thereby driving the first screw rod 84 to rotate forward, so that the first movable plate 85, the electric liquid suction device 86 and the electric suction cup 87 move downward, so that the electric suction cup 87 is moved downward. 7 contacts the transwell chamber 16, then controls the output shaft of the second motor 83 to stop forward rotation, then controls the electric suction cup 87 to open, so that the electric suction cup 87 sucks the transwell chamber 16, then controls the output shaft of the second motor 83 to reverse, thereby driving the first screw rod 84 to rotate forward, so that the first movable plate 85, the electric suction device 86 and the electric suction cup 87 move upward and reset, so that the electric suction cup 87 sucks the transwell chamber 16 to move upward, and then controls the output shaft of the second motor 83 to stop reverse rotation Then, the output shaft of the first motor 81 is controlled to reverse, thereby driving the guide frame 82, the toothed plate 1105, the first movable plate 85, the electric liquid suction device 86 and the electric suction cup 87 to reverse, so that the front toothed plate 1105 drives the front second gear 1104 to reverse and reset, thereby driving the front rotating rod 1101 and the front baffle 1102 to reverse and reset, and the elastic member 1103 returns to its original state. When the front toothed plate 1105 is separated from the front second gear 1104, the front second gear 1104, the front rotating rod 1101 and the front baffle 1102 are The baffle 1102 stops reversing, and when the guide frame 82, the tooth plate 1105, the first movable plate 85, the electric suction device 86 and the electric suction cup 87 are reversed and reset, they will continue to reverse, so that the electric suction cup 87 is reversed to the top of the glass cup 14, and then the output shaft of the first motor 81 is controlled to stop reversing, and then the output shaft of the second motor 83 is controlled to rotate forward again, so that the electric suction device 86 and the electric suction cup 87 move downward, so that the electric suction cup 87 drives the transwell chamber 16 into the glass cup 14. When the transwell After the transwell chamber 16 contacts the glass 14, the output shaft of the second motor 83 is controlled to stop rotating forward, and then the electric suction cup 87 is controlled to be closed, so that the electric suction cup 87 releases the transwell chamber 16. The output shaft of the second motor 83 is controlled to be reversed again, so that the electric suction device 86 and the electric suction cup 87 move upward and reset, thereby separating the electric suction cup 87 from the transwell chamber 16. Then the output shaft of the first motor 81 is controlled to rotate forward and reset, thereby driving the guide frame 82, the gear plate 1105, the first movable plate 85, the electric suction device 86 and the electric suction cup 87 to rotate forward and reset, so that the electric suction device 86 is again directly above the glass 14. Then the above operation is repeated to complete the drug liquid loading again, so that the electric suction device 86 sprays the drug liquid into the transwell chamber 16. Then the first motor 81 and the second motor 83 are turned off, and the third motor 92 is turned on. The output shaft of the third motor 92 is controlled to rotate forward, thereby driving the second screw rod 93 to rotate forward, so that the second movable plate 94 and the conductivity tester 7 move downward, so that one end of the conductivity tester 7 is inserted into The other end of the conductivity tester 7 is inserted into the liquid medicine in the glass cup 14, and the other end of the conductivity tester 7 is inserted into the liquid medicine in the transwell chamber 16, and then the third motor 92 is turned off, and the conductivity tester 7 and the display 4 are turned on, so that the conductivity tester 7 starts to detect, and the detected data will be displayed on the display 4, and then people can get the detection result by watching the display 4. When the conductivity tester 7 completes the detection, the conductivity tester 7 and the display 4 are turned off, and the third motor 92 is turned on again, and the output shaft of the third motor 92 is controlled to reverse, thereby driving The second screw rod 93 is reversed to move the second movable plate 94 and the conductivity tester 7 upward and reset, and then the third motor 92 is turned off, and the movable door 3 is pulled to rotate and open, and then the glass cup 14 is taken out from the lower part of the support frame 91, and then the baffle 1102 is pushed to rotate, and the elastic member 1103 is deformed, and then the liquid medicine tube 15 filled with liquid medicine is taken out from the second placement rack 6, and then the baffle 1102 is released, and the elastic member 1103 returns to its original state, and the elastic member 1103 drives the baffle 1102 to reverse and reset, and then the movable door 3 is pushed to reverse and close.

Example 2

On the basis of Example 1, a cleaning mechanism 12 is also included, and the cleaning mechanism 12 includes a support rod 1202 and a wiping cotton 1203. Referring to Figures 2 and 10, an annular groove 1201 is opened on the outer side of the top of the base 1, and the annular groove 1201 is located inside the isolation cover 2. A support rod 1202 is welded to the lower rear part of the slider 102, and the support rod 1202 slides in the annular groove 1201. The rear side of the support rod 1202 is connected to a wiping cotton 1203. The inner wall of the isolation cover 2 can be wiped by the movement of the wiping cotton 1203, and the wiping cotton 1203 is in contact with the inner wall of the isolation cover 2.

When the slider 102 moves in a circular motion, the slider 102 drives the support rod 1202 and the wiping cotton 1203 to move in a circular motion, so that the wiping cotton 1203 wipes the inner wall of the isolation cover 2, thereby making it convenient for people to observe the internal situation through the isolation cover 2. When the slider 102 stops moving, the support rod 1202 and the wiping cotton 1203 stop moving.

It also includes a blocking mechanism 13, which includes a protective shell 1301 and a rubber sleeve 1302. Referring to Figures 2 and 11, a protective shell 1301 is welded to the upper inner part of the annular rack 101, and the outer side of the protective shell 1301 is connected to the isolation cover 2. The guide frame 82 is connected to the rubber sleeve 1302. Under the action of the protective shell 1301 and the rubber sleeve 1302, the fourth motor 103 and the first screw rod 84 can be protected, and the left and right sides of the first movable plate 85 pass through the rubber sleeve 1302.

When people use the endothelial cell monolayer permeability detection experimental device, the fourth motor 103 can be protected under the action of the protective shell 1301. When the hydrogen peroxide sterilizer 105 is sterilizing, the rubber sleeve 1302 can protect the first screw rod 84. When the first movable plate 85 moves, the rubber sleeve 1302 is deformed. When the first movable plate 85 is reset, the rubber sleeve 1302 returns to its original state.

Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims (7)

1. An endothelial cell monolayer permeability detection experimental device, comprising a base (1), an isolation cover (2), a movable door (3) and a conductivity tester (7), wherein the top of the base (1) is connected to the isolation cover (2), the front of the isolation cover (2) is rotatably connected to the movable door (3), and the conductivity tester (7) is used for experimental detection, characterized in that it also comprises a display (4), a first placement rack (5), a second placement rack (6), a control mechanism (8), a testing mechanism (9), a sterilization mechanism (10) and an isolation mechanism (11), wherein the upper front side of the isolation cover (2) is connected to the display (4), the right front side of the top of the base (1) is connected to the first placement rack (5), the right rear side of the top of the base (1) is connected to the second placement rack (6), the first placement rack (5) and the second placement rack (6) are both located inside the isolation cover (2), the upper side of the base (1) is provided with a control mechanism (8) for automatic material preparation, the rear side of the top of the base (1) is provided with a control mechanism (8) for automatic material preparation, and the upper side of the base (1) is provided with a control mechanism (8) for automatic material preparation. A testing mechanism (9) for moving the conductivity tester (7) is provided in the middle, the conductivity tester (7) is mounted on the testing mechanism (9), the conductivity tester (7) is electrically connected to the display (4), a sterilizing mechanism (10) for sterilizing is provided on the upper inner side of the isolation cover (2), an isolation mechanism (11) for blocking the experimental instrument is provided between the first placement rack (5) and the second placement rack (6), the isolation cover (2) and the movable door (3) are used to form a closed space by placing the experimental instrument on the first placement rack (5) and the second placement rack (6), the experimental instrument is then blocked by the isolation mechanism (11), the closed space is then sterilized by the sterilizing mechanism (10), the experimental instrument and the liquid medicine are prepared by the control mechanism (8), the conductivity tester (7) is then driven to move by the testing mechanism (9), the conductivity tester (7) starts to detect, and the display (4) is used to display the detection data; The control mechanism (8) comprises a first motor (81), a guide frame (82), a second motor (83), a first screw rod (84), a first movable plate (85), an electric liquid suction device (86) and an electric suction cup (87). The first motor (81) is connected to the right side of the middle portion of the upper side of the base (1). The guide frame (82) is rotatably connected to the right side of the middle portion of the upper side of the base (1). The bottom of the guide frame (82) is connected to the output shaft of the first motor (81). The top of the guide frame (82) is connected to the second motor (83). The first screw rod (84) is rotatably connected to the upper side of the guide frame (82). 84) is connected to the output shaft of the second motor (83) at the top, a first movable plate (85) is slidably connected to the guide frame (82), the first movable plate (85) is threadedly connected to the first screw rod (84), an electric liquid suction device (86) is connected to the left side of the first movable plate (85), and an electric suction cup (87) is connected to the right side of the first movable plate (85), the electric liquid suction device (86) and the electric suction cup (87) are driven to rotate and move by turning on the first motor (81) and the second motor (83), and then the electric liquid suction device (86) and the electric suction cup (87) are turned on to realize automatic material preparation; The testing mechanism (9) comprises a support frame (91), a third motor (92), a second screw rod (93) and a second movable plate (94); the support frame (91) is connected to the middle of the top rear side of the base (1); the third motor (92) is connected to the top of the support frame (91); the second screw rod (93) is rotatably connected inside the support frame (91); the top of the second screw rod (93) is connected to the output shaft of the third motor (92); the second movable plate (94) is threadedly connected to the second screw rod (93); the second movable plate (94) is slidably connected to the support frame (91); the front of the second movable plate (94) is connected to the conductivity tester (7); the third motor (92) is turned on to drive the second screw rod (93) to rotate, so that the second movable plate (94) and the conductivity tester (7) move; The isolation mechanism (11) comprises a rotating rod (1101), a baffle (1102), an elastic member (1103), a second gear (1104) and a toothed plate (1105); the outer sides of the tops of the first placement rack (5) and the second placement rack (6) are both rotatably connected to the rotating rod (1101); the lower sides of the rotating rods (1101) are both connected to the baffle (1102); the elastic member (1103) is connected between the front baffle (1102) and the first placement rack (5); and the rear baffle (1102) and the second placement rack are connected to each other. (6) is also connected with an elastic member (1103), the upper side of the rotating rod (1101) is connected with a second gear (1104) via a one-way clutch, and the upper left side and the top right side of the guide frame (82) are connected with a toothed plate (1105), and the toothed plate (1105) will contact the second gear (1104) when rotating, and the experimental instrument is placed by pushing the baffle (1102) to rotate, and the elastic member (1103) is used to reset the baffle (1102) so that the baffle (1102) covers the experimental instrument. 2. An endothelial cell monolayer permeability detection experimental device as claimed in claim 1, characterized in that the sterilization mechanism (10) comprises an annular rack (101), a slider (102), a fourth motor (103), a first gear (104) and a hydrogen peroxide sterilizer (105); the upper inner part of the isolation cover (2) is connected with an annular rack (101); the inner side of the annular rack (101) is slidably connected with the slider (102); the upper part of the slider (102) is connected with the fourth motor (103); the output shaft of the fourth motor (103) is connected with the first gear (104); the first gear (104) is meshed with the annular rack (101); the front side of the slider (102) is connected with the hydrogen peroxide sterilizer (105); when the fourth motor (103) is turned on, the first gear (104) is driven to rotate, so that the slider (102) and the hydrogen peroxide sterilizer (105) move; and then the hydrogen peroxide sterilizer (105) is turned on to achieve sterilization of the inside of the isolation cover (2). 3. An endothelial cell monolayer permeability detection experimental device as described in claim 1, characterized in that it also includes a cleaning mechanism (12) for wiping the isolation cover (2), the cleaning mechanism (12) includes a support rod (1202) and a wiping cotton (1203), the annular slide groove (1201) is located inside the isolation cover (2), the lower rear part of the slider (102) is connected to the support rod (1202), the rear side of the support rod (1202) is connected to the wiping cotton (1203), the wiping cotton (1203) is in contact with the inner wall of the isolation cover (2), and the support rod (1202) and the wiping cotton (1203) move to make the wiping cotton (1203) wipe the inner wall of the isolation cover (2). 4. An endothelial cell monolayer permeability detection experimental device as described in claim 3, characterized in that it also includes a blocking mechanism (13) for protection, the blocking mechanism (13) includes a protective shell (1301) and a rubber sleeve (1302), the upper inner part of the annular rack (101) is connected to the protective shell (1301), the outer side of the protective shell (1301) is connected to the isolation cover (2), the guide frame (82) is connected to the rubber sleeve (1302), the left and right sides of the first movable plate (85) both pass through the rubber sleeve (1302), and the fourth motor (103) and the first screw rod (84) are protected by the protective shell (1301) and the rubber sleeve (1302). 5. An endothelial cell monolayer permeability detection experimental device as claimed in claim 1, characterized in that the isolation cover (2) is made of transparent material. 6. An endothelial cell monolayer permeability detection experimental device as claimed in claim 1, characterized in that the elastic member (1103) is a torsion spring. 7. An endothelial cell monolayer permeability detection experimental device as claimed in claim 3, characterized in that an annular groove (1201) is opened on the outer side of the top of the base (1), and the support rod (1202) slides in the annular groove (1201).