CN114983610A - A measuring device for animal experiments of tumor electric field therapy - Google Patents

Abstract

The invention discloses a measuring device for tumor electric field treatment animal experiments, which is a device for measuring the electric field intensity in an animal body in tumor electric field treatment (TTfields) experiments. The locator is fixed, the experimental animal of location, keeps fixed position, and multichannel equidistance measurement probe passes through the measuring hole on the locator, and in getting into the animal body, the electric field intensity of quantitative measurement different positions and degree of depth, different measurement channel signals of channel selector selection are transmitted to display recording device. The device has reasonable design, high measurement precision and strong practicability and reliability, and plays an important role in optimizing animal experiment schemes related to tumor electric field treatment and optimizing simulation models.

Description

A measuring device for animal experiments of tumor electric field therapy

technical field

The invention relates to the technical field of experimental equipment, in particular to a measuring device for animal experiments of tumor electric field therapy.

Background technique

Tumor-Treating Fields (TTFields) is a new type of non-invasive, anti-mitotic tumor treatment technology. Inhibit its mitotic process to achieve the effect of tumor treatment. In May 2020, tumor electric field therapy was approved for marketing in China. However, the mechanism by which TTFields inhibit the proliferation of tumor cells is still unclear, which makes the related treatment technologies have great limitations in tumor treatment, especially for other different types of tumor treatments, which are still being further explored.

In order to study the effect and mechanism of TTFields on different tumor cells, in addition to tumor cell experiments, animal experiments are an important experimental link. TTFields experiments with different parameters are carried out for different tumor animal models; TTFields stimulate electrodes to apply an AC electric field to animals. In vivo, in order to ensure sufficient electric field strength, it is necessary to analyze and calculate the entire stimulation circuit to ensure an electric field strength of 1-2V/cm in animals. The length of the animal is used to adjust the voltage, and there has been no relevant measurement on whether the required electric field intensity is generated in the animal. On the other hand, due to the special electrical properties of biological tissues, parameters such as conductivity, relative permittivity, and density are very different, coupled with the influence of blood and tissue fluid flow, muscle contraction and peristalsis, etc., the electric field generated by TTFields in vivo The magnitude and distribution of intensities are bound to vary enormously. At present, computer modeling and simulation analysis are generally used for the magnitude and distribution of electric field in vivo. This method establishes a layered model according to different biological tissues, and assigns appropriate electrical conductivity and relative permittivity to different tissues. Finally, the simulated electric field is obtained. intensity distribution. Whether the magnitude and distribution of the actual electric field in vivo is consistent with the results of computer modeling and simulation has not been confirmed. At the same time, due to the special electrical properties of biological tissues, the value of each parameter varies widely, and whether the parameter selection in the modeling process is accurate is also an issue. an unresolved issue.

Therefore, a device that can reliably measure the treatment intensity of tumor electric field in vivo will have important positive significance for animal experiments and computer accurate modeling and simulation related to tumor electric field treatment.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a measuring device for animal experiments of tumor electric field treatment, which is a measuring device of electric field intensity in animals in tumor electric field treatment experiments. It can solve the problem that the size and distribution of the actual electric field intensity in the animal body cannot be determined in the current TTFields animal experiment and computer modeling simulation, and provide necessary real data for further optimization of experimental methods and parameters.

The technical means adopted by the present invention to solve its problems are as follows: the described measurement device for animal experiments on tumor electric field treatment mainly includes three parts: a multi-channel isometric measurement probe, a locator and a channel selector. The multi-channel equidistant measurement probe passes through the measurement hole of the locator and enters the body of the animal to be measured to measure the electric field strength. The channel selector selects to open the measurement channel on the multi-channel equidistant measurement probe, and transmits the measurement signal to the display and recording end.

The multi-channel equidistant measurement probe is mainly composed of a conductive site, a probe body and a transmission wire. The multi-channel equidistant measurement probe is in the shape of a conical cylinder as a whole, and is divided into hollow and solid according to different materials of the probe body. There are two structures. The diameter of the probe can be selected from 0.5mm to 4mm according to the size of the animal to be tested and the tissue. It is the main rigid body structure of the entire probe, which can be made of insulated high-strength metal materials and high-strength insulating plastics. When the probe body is made of insulated high-strength metal materials (such as stainless steel), the probe is Hollow structure, the transmission wire is routed in the middle cavity of the probe body, passes through the reserved pores under the coverage area of the conductive site, and is connected to the conductive site by welding; when the probe body is made of high-strength insulating plastic, the probe The whole is a cast solid structure. The conductive sites and transmission wires are first welded and fixed in the mold for preparing the probe body, and then made of plastic; The depth of the probe; the conductive sites are made of materials with superior conductivity and easy to process into foil, such as: gold, silver, copper, etc., which are fixed on the surface of the probe body by sticking or pouring, and the adjacent sites, etc. The distance between them can be 1mm-5mm according to different size animals; the transmission wire is a material with excellent electrical conductivity that has been insulated on the surface, connecting the conductive point and the channel selector, and the transmission wire is routed inside the probe body and at the exit It is converted into a separate wire and connected to the channel selector input port.

The locator is made of transparent plastic material and is divided into an upper locator and a lower locator. There is a corresponding groove in the middle of the upper locator and the lower locator. Space, the shape of the groove fits the shape of the animal's trunk, and the size of the groove varies according to different types of experimental animals, and is generally set to the average trunk size of the animal to be tested. The upper locator and the lower locator are combined by four groups of tenon-and-mortise structures. Two corresponding magnetic suction devices are embedded on the butting surface of the mortise-and-mortise structure. The surface of the upper locator has a cross scale line indicating a millimeter scale; the upper locator is crossed in the center. The scale line is provided with a number of equidistant measurement holes with a mutual distance of 5mm. The equidistant measurement holes pass through the upper locator. The diameter of the equidistant measurement holes is slightly larger than the diameter of the main body of the multi-channel equidistant measurement probe. The multi-channel equidistant probe passes through the equidistant. Distance measurement holes to achieve quantitative measurement of positioning. The number of isometric measurement holes is at least 5 in the direction of the long axis of the torso, and at least 3 in the direction of the transverse axis of the torso. The maximum number varies according to the size of the experimental animal; There is a stimulating electrode fitting groove 28 for installing and fixing electrodes for applying an electric field.

The channel selector is a circuit design module, which mainly includes a power supply module, a control module, an input module, an output module and a display module, wherein the power supply module converts the DC 5v power supply into different voltages for use by other modules; the control module realizes the main timing , gating function; the input module is connected to the multi-channel signal of the probe; the output module transmits the gating signal to the display and recording terminal, which can be transmitted in two different transmission modes: wired transmission and wireless transmission; the display module displays the current recording time and the gating channel No.

The multi-channel isometric measurement probe of the present invention has: (1) tough texture, no biological toxicity and side effects, and good biocompatibility; (2) the probe is small, and the detection site on the needle has good electrical conductivity; (3) ) The detection sites of each channel are equidistant, and the penetration depth can be observed.

The positioner of the present invention has: (1) a transparent structure, which can observe the measurement probe and the surface features of the animal; (2) has an equidistant measurement hole for insertion and measurement of multi-channel equidistant measurement probes; (3) has The center mark line is aligned with the animal body position; (4) It has an electrode groove that generates an electric field, and is suitable for installing stimulating electrodes; (5) It has a tenon-and-mortise structure, which can be combined accurately and stably; (6) There is no actual size limit, and it can be proportional to the size of the experimental animal Adjustment.

The channel selector of the present invention has: (1) cyclically selects a certain measurement channel in the multi-channel equidistant probe; (2) can set the measurement time length, and transmit it to the display and record end without damage; (3) display The current gated channel and recording duration.

Compared with the current overall electric field strength calculation method and computer modeling simulation analysis electric field strength method based on the whole experimental animal, the present invention has the following advantages: (1) the electric field strength in the body of the real experimental animal can be directly measured; (2) Using the measuring holes of the positioner and the multi-channel equidistant measurement probes, the spatial distribution of the electric field intensity on the body surface and in the body can be measured; (3) The positioner has wide applicability and can be used for experimental animals of different types and sizes (4) Channel selection (5) The whole set of measuring device has reasonable structure design, strong applicability and high reliability, which is of great significance for promoting in-depth research on scientific issues.

Description of drawings

Figure 1 is a schematic structural diagram of the present invention.

FIG. 2 is a schematic structural diagram of a multi-channel equidistant measurement probe.

Figure 3 is a schematic diagram of the structure of the positioner.

FIG. 4 is a schematic diagram of the structure of the channel selector.

FIG. 5 is a diagram showing an example of practical application of the present invention.

Detailed ways

The present invention will be further described with reference to the accompanying drawings and embodiments.

Example 1

Referring to FIG. 1 , it is a measurement device used for tumor electric field treatment animal experiments according to the present invention, which mainly includes three parts: a multi-channel isometric measurement probe 1 , a positioner 2 and a channel selector 3 .

Referring to FIG. 2 , the multi-channel equidistant measurement probe 1 is mainly composed of a conductive site 11 , a probe body 12 and a transmission wire 13 , and the multi-channel equidistant measurement probe has a conical cylindrical shape as a whole. The material is divided into two structures: hollow and solid. The diameter of the probe can be selected from 0.5mm to 4mm according to the size and tissue of the animal to be measured. The multi-channel isometric measurement probe is designed with a pointed tip, which can be broken The tissue to be tested; the probe body 12 is the main rigid structure of the entire probe, and can be made of insulated high-strength metal materials and high-strength insulating plastics. When the probe body 12 is used, the probe is a hollow structure, and the transmission wire 13 is routed in the middle cavity of the probe body 12, passes through the reserved pores under the coverage area of the conductive site (11), and is connected to the conductive site 11 by welding; When high-strength insulating plastic is used to prepare the probe body 12, the probe as a whole is a cast solid structure. First, the conductive sites 11 and the transmission wires 13 are welded and fixed in the mold for preparing the probe body 12, and then the plastic is cast to make the probe body 12. ; The tail of the probe body 12 has a scale mark 14, which can measure the depth of the probe into the positioner 2; the conductive site 11 is made of materials with superior conductivity and easy to process into foil, such as: gold, silver, Copper, etc., are fixed on the surface of the probe main body 12 by the method of pasting or casting, and the adjacent points are equidistant. According to different animals, the distance can be 1mm-5mm; the transmission wire 13 is insulated on the surface and has excellent conductivity The material is connected to the conductive site 11 and the channel selector 3, the transmission wire 13 is routed inside the probe body 12, converted into an independent wire at the outlet, and connected to the channel selector input port.

Referring to FIG. 3 , the positioner 2 is made of transparent plastic material, and is divided into an upper positioner 21 and a lower positioner 22 . There is a corresponding groove 23 in the middle of the upper positioner 21 and the lower positioner 22 , and the groove 23 is in the positioner After the combination is fixed, a space for accommodating the animal's torso is formed. The shape of the groove 23 matches the shape of the animal's torso, which can well accommodate and fix the experimental animals. Average trunk size, at the time of the experiment, select experimental animals whose trunk size matches. The upper locator 21 and the lower locator 22 are combined by four groups of tenon-and-mortise structures 24, and two corresponding magnetic attraction devices 25 are embedded on the butting surface of the tenon-and-mortise structure 24 to ensure that the two parts are tightly combined, and the surface of the upper locator 21 has a cross scale line. 26. Mark the millimeter scale, which is convenient for positioning the animal; the upper locator 21 is provided with a number of equidistant measuring holes 27 on the central cross scale line, with a mutual distance of 5mm, which penetrates the upper locator 21, and the diameter of the measuring hole 27 is slightly larger than that of the multi-channel equidistant measurement. The diameter of the probe body 12, the multi-channel equidistant probe 1 passes through the equidistant measurement hole 27 to achieve quantitative measurement of positioning, the number of equidistant measurement holes 27 is at least 5 in the longitudinal direction of the trunk, and at least 3 in the transverse axis direction of the torso. The maximum number varies according to the size of the experimental animal; there are stimulating electrode fitting grooves 28 on both sides of the middle groove of the lower positioner 22 for installing and fixing electrodes for applying electric field.

Referring to FIG. 4, the channel selector 3 is a circuit design module, which mainly includes a power module 31, a control module 32, an input module 33, an output module 34 and a display module 35, wherein the power module 31 converts the DC 5v power supply into different voltages for other The control module 32 realizes the main timing and gating functions; the input module 33 connects the probe multi-channel signal; the output module 34 transmits the gated signal to the display and recording terminal, which can be transmitted through wired transmission and wireless transmission. mode; the display module 35 displays the current recording time and the gated channel number.

Example 2

Referring to FIG. 5, it is a schematic diagram of an application example of the present invention. In the present invention, the locator 2 locates and installs the experimental animal, wherein the stimulating electrode and the lead wire are installed in the fitting groove before the animal is fixed, and the end of the lead wire is connected to the TTFields device through the corresponding interface. Enter the lower part of the positioner 2, the trunk between the front and rear limbs is placed in the positioner groove, the limbs and other parts are located outside the positioner, and the center line of the animal trunk and the scale line of the positioner 2 are aligned. Merging the upper and lower parts of the positioner 2, the multi-channel equidistant measurement probe 1 extends into the positioner 2 and the inside of the animal body through the measurement hole of the positioner 2 until the predetermined depth; the transmission wire at the tail end of the multi-channel equidistant measurement probe 1 Access the input port of the channel selector 3, connect the output port of the channel selector 3 to the measurement signal display and recording device 4 (such as an oscilloscope), open the TTFields device, adjust the experimental parameters, display and record the multi-channel isometric measurement probe 1 For the signal at the preset position, the electric field at different positions and depths in the experimental animal is repeatedly measured through different measurement holes, and finally the overall electric field intensity distribution is obtained.

Claims (5)

1. The utility model provides a measuring device for tumour electric field treatment animal experiment, its characterized in that mainly includes three parts of multichannel equidistance measuring probe (1), locator (2) and channel selector (3), multichannel equidistance measuring probe (1) passes equidistance measuring hole (27) in locator (2) and gets into the internal measured electric field intensity of animal that awaits measuring, and channel selector (3) are through the measuring channel on multichannel equidistance measuring probe (1), with measuring signal transmission to showing the record end.

2. The measuring device according to claim 1, wherein the multichannel equidistant measurement probe (1) is composed of a conductive site (11), a probe body (12) and a transmission line (13), the multichannel equidistant measurement probe (1) is integrally conical and cylindrical, the diameter is 0.5mm to 4mm, the head end of the multichannel equidistant measurement probe (1) is designed to be a pointed end, the tail part of the probe body (12) is provided with a scale mark (14), the conductive site (11) is made of a material which has excellent conductivity and is easy to process into a foil, the conductive site is fixed on the surface of the probe body (12) by adopting an integrated pasting or casting method, adjacent sites are distributed at equal intervals of 1mm to 5mm, the transmission line (13) is a conductive material with insulated appearance and is connected with the conductive site (11) and the channel selector (3), the transmission line (13) is arranged inside the probe body (12), and the output end of the channel selector is converted into a separate conductor and is connected with the input port of the channel selector (3).

3. The measuring device according to claim 2, characterized in that the probe body (12) is a main rigid structure of the whole probe, the probe body (12) is divided into a hollow structure and a solid structure, and is respectively made of insulated high-strength metal material or high-strength insulating plastic; when the probe main body (12) is prepared by adopting an insulated high-strength metal material, the probe main body (12) is of a hollow structure, a transmission lead (13) is wired in a middle cavity of the probe main body (12), passes through a reserved hole below a coverage area of the conductive site (11), and is connected with the conductive site (11) in a welding way; when the probe (12) is made of high-strength insulating plastic, the probe body (12) is of a cast solid structure, the conducting sites (11) and the transmission leads (13) are welded and fixed in a mold of the probe body (12), and then the probe is made of the cast plastic.

4. The measuring device according to claim 1, characterized in that the positioner (2) is made of transparent plastic material, and is divided into an upper positioner (21) and a lower positioner (22), a corresponding groove (23) is arranged between the upper positioner (21) and the lower positioner (22), the groove (23) forms a space for accommodating the animal trunk after the positioners are combined and fixed, the shape of the groove (23) is matched with the shape of the animal trunk, the size of the groove (23) is changed according to different types of experimental animals and is generally set to be the average size of the trunk of the animal to be measured, the upper positioner (21) and the lower positioner (22) are combined through four groups of tenon-and-mortise structures (24), two corresponding magnetic suction devices (25) are embedded on the butt-joint surface of the tenon-and-mortise structures (24), the surface of the upper positioner (21) is provided with cross scale lines (26), the upper positioner (21) is provided with a plurality of equidistant measuring holes (27) with a mutual distance of 5mm on the central cross scale line, the equidistant measuring holes (27) penetrate through the upper positioner (21), the diameter of the equidistant measuring holes (27) is larger than that of the multichannel equidistant measuring probe (1), the multichannel equidistant measuring probe (1) penetrates through the equidistant measuring holes (27), positioning and quantitative measurement are realized, the number of the equidistant measuring holes (27) is at least 5 in the long axis direction of the trunk, at least 3 in the transverse axis direction of the trunk, and the maximum number is changed according to the size of an experimental animal; stimulation electrode fit grooves (28) are arranged on two sides of the middle groove of the lower locator (22) and used for installing and fixing electrodes for applying an electric field.

5. The measuring device according to claim 1, wherein the channel selector (3) is a circuit design module, mainly comprising a power module (31), a control module (32), an input module (33), an output module (34) and a display module (35), wherein the power module (31) converts a direct current 5v supply into a different voltage for use by other modules; the control module (32) realizes the main timing and gating functions; the input module (33) is connected with the probe multichannel signal; the output module (34) transmits the gated signal to the display recording end, and two different transmission modes are adopted through wired transmission and wireless transmission; the display module (35) displays the current recording time and the gating channel number.

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