CN114958828B - Data information storage method based on DNA molecule medium - Google Patents

Abstract

The invention relates to a data information storage method based on DNA molecular medium, in particular to a recombinant plasmid library composed of recombinant plasmids containing general information storage DNA, wherein the plasmids in the plasmid library use base joints as base sequences for recording information. The recombinant plasmid library can be repeatedly applied to the storage of different data information in DNA molecular media, can be prepared for multiple times, reduces the proportion of chemical synthesis, and realizes more economic, rapid and green DNA data storage.

Description

Data information storage method based on DNA molecule medium

Technical Field

The present invention belongs to the technical field of data storage, and in particular relates to a data storage method, device, equipment and a readable storage medium based on a DNA molecule medium.

Background technique

With the development of the Internet, big data, artificial intelligence and other human civilization technologies, global data is growing exponentially. Traditional storage methods such as hard disks, tapes, and optical disks cannot meet the growing demand for massive data storage due to high maintenance costs, large space occupation, and short storage life.

As a data storage medium, DNA has the characteristics of high storage density, long storage time and low maintenance cost. It is calculated that 1g of DNA can store data equivalent to 1 million high-definition movies. At the same time, as a material of life genetic information, DNA can be inserted into the cells of animal, plant and microorganisms, and through the replication of living organisms, it can achieve permanent storage from generation to generation.

The current DNA data storage method includes the following steps: 1) encoding of data information to DNA sequence; 2) synthesis of DNA sequence; 3) sequencing and interpretation of synthetic DNA; 4) decoding of DNA sequence to data information obtained by sequencing. In 2012, George Church et al. successfully realized the data storage of 53,426 words, 11 JPG pictures and 1 Java program in synthetic DNA using DNA medium. In 2013, Goldman et al. stored 757,051 bytes of data information in different forms in synthetic DNA medium. In 2018, Microsoft and the University of Washington jointly published an article in the journal Nature Biotechnology, successfully realizing the storage of 200Mb data in synthetic DNA. These research works all use disposable synthetic DNA libraries to store data. The number of computer bits stored and the synthetic bases correspond to each other in the form of 1 base = 1~2 bits of data, that is, the amount of computer binary data stored requires at least half of the corresponding amount of synthetic bases to be stored. This storage method is expensive due to the high cost of DNA synthesis (the current commercial price is about 0.01 yuan to 1 yuan per nt, depending on the different synthesis methods). Synthetic DNA can only be used once to store the corresponding data, making the final storage cost of DNA data high.

How to significantly reduce the cost of DNA data storage is the main challenge currently facing this field.

Summary of the invention

The problem to be solved by the present invention is that the current cost of DNA data storage is high, and the expensive synthetic DNA in DNA data storage cannot be reused. In view of this problem, the present invention proposes a method, based on biological fermentation, to prepare a universal plasmid DNA library that can be repeatedly called, and to use enzyme assembly to realize in vitro or in vivo storage of any information in the plasmid DNA, so that the raw DNA can be repeatedly called, and the data information can be stored in vitro or in vivo.

One aspect of the present invention provides a method for preparing a recombinant plasmid containing universal information storage DNA, the preparation method comprising the following steps:

S01): designing a universal information DNA sequence module, which comprises two groups of base linkers and a linker arm sequence located between the two groups of base linkers, connecting the two groups of base linkers and the linker arm sequence located between the two groups of base linkers to obtain a base linker core sequence; adding a second type of restriction endonuclease linker sequence to both ends of the base linker core sequence to obtain a universal information DNA sequence module sequence;

S02): synthesizing a universal information DNA sequence module and inserting it into a plasmid vector to obtain a recombinant plasmid;

Optionally, S03): the recombinant plasmid is introduced into a host bacterium for culture and preservation, or the recombinant plasmid containing the universal information DNA sequence is extracted from the host bacterium for preservation.

Furthermore, the base linker is a set formed by any combination of bases, and the base linker is used to record information.

Furthermore, the linker arm sequence is a known and determined base sequence.

Furthermore, the two groups of base linkers have n1 bases and n2 bases respectively, and n1 and n2 are independently selected from any integer of 3-10, for example, independently selected from 3, 4, 5, 6, 7, 8, 9, 10.

Another aspect of the present invention provides a recombinant plasmid containing universal information storage DNA, which is prepared by the above method; or

The recombinant plasmid contains a universal information DNA sequence module sequence, and the universal information DNA sequence module contains two groups of base linkers and a linker arm sequence located between the two groups of base linkers. The two groups of base linkers and the linker arm sequence located between the two groups of base linkers are connected to obtain a base linker core sequence; and there are also two types of restriction endonuclease linker sequences on the left and right ends of the base linker core sequence.

In another aspect, the present invention provides a recombinant plasmid library, which comprises a plurality of the above-mentioned recombinant plasmids containing universal information storage DNA.

Furthermore, the recombinant plasmid library comprises 4 ^(n1+n2) types of recombinant plasmids containing universal information storage DNA with different base linkers, and n1 and n2 are the base lengths of the first base linker and the second base linker in the two groups of base linkers, respectively.

The n1 and n2 are independently selected from any integer of 3-10, for example, independently selected from 3, 4, 5, 6, 7, 8, 9, 10.

Furthermore, the sequences of the linker arms of the recombinant plasmids in the same recombinant plasmid library are identical.

Furthermore, the length of the recombinant plasmid linker arm sequence in the same recombinant plasmid library is 3-100 bases, for example, 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100 bases.

In another aspect, the present invention provides a DNA storage method for data, the DNA storage method comprising the following steps:

S11) extracting binary information of computer data information to be stored;

S12) converting the binary information into a base sequence for information recording according to a preset mapping relationship;

S13) splitting the information recording base sequence into short base segments, and providing overlapping base sequences between adjacent short base segments; or

There are also overlapping base sequences between the short base segments composed of adjacent odd-numbered bit sequences and between the short base segments composed of adjacent even-numbered bit sequences;

S14) splitting each of the short base fragments obtained by splitting in step S13) from the middle, so that each short base fragment is split into a first short base fragment and a second short base fragment;

S15) preparing the required recombinant plasmid with a universal information DNA sequence for information recording by the above-mentioned preparation method of the present invention or selecting from the recombinant plasmid library containing universal information storage DNA of the present invention; the first base linker on the recombinant plasmid with a universal information DNA sequence for information recording is the same as the first short base fragment designed in step S14), and the second base linker is the same as the second short base fragment obtained in step S14); obtaining a recombinant plasmid with a universal information DNA sequence corresponding to all the short base fragments split in step S13);

S16) using restriction endonucleases to digest the recombinant plasmid obtained in step S15), collecting the digested fragments, and connecting them into a complete information recording fragment using a ligase;

S17) preparing a recombinant plasmid having a universal information DNA sequence for a vector by the preparation method of the present invention or selecting from the recombinant plasmid library containing universal information storage DNA of the present invention; the first base linker on the recombinant plasmid having a universal information DNA sequence for a vector is the same as the first short base fragment of the 5' end short base fragment of the information recording base sequence designed in step S13), and the second base linker is the same as the second short base fragment of the 3' end short base fragment of the information recording base sequence designed in step S13);

S18) using restriction endonuclease to digest the recombinant plasmid obtained in step S17), collecting the digested plasmid as a vector plasmid, inserting the information recording fragment obtained in step S16) into the vector plasmid, and obtaining a recombinant plasmid for recording information.

Furthermore, the recombinant plasmid with recorded information obtained in step S18) is directly freeze-dried and stored in vitro.

Furthermore, the recombinant plasmid containing the recorded information obtained in step S18) is transferred into the host bacteria and replicated and stored.

Furthermore, the recombinant plasmid recording the information obtained in step S18) is transferred into the host bacteria, replicated, and then extracted and stored in the form of freeze-dried powder.

Furthermore, the host bacteria is any microorganism that can replicate and preserve the recombinant plasmid, and is more preferably Escherichia coli, yeast, lactobacillus, bifidobacterium, or enterococcus.

Furthermore, in step S11), the computer data information can be data information existing on the computer, preferably selected from pictures, texts, programs, audios, and videos.

Furthermore, when the base sequence is divided into a plurality of short base fragments, when the sequence length of the terminal short base fragment is shorter than that of other short base fragments, repeated bases are used to fill the sequence length to be consistent with that of other short base fragments.

Furthermore, in step S12), before the base sequence for information recording is split into short base fragments, the base sequence for information recording is also split into a plurality of sub-base sequences for information recording, and an index fragment is added before each sub-base sequence for information recording. Preferably, the length of the index fragment sequence is 3-100 bases, for example, 3, 4, 5, 6, 7, 8, 9, 10 bases. The index fragment added before each fragment is different, and in S13), the sub-base sequences for information recording connected with different index fragments are split into short base fragments respectively.

Furthermore, after the information recording base sequence is split into sub-information recording base sequences, in S16), a complete information recording segment is prepared corresponding to each sub-information recording base sequence.

Furthermore, after the information recording base sequence is split into sub-information recording base sequences, in S18), the information recording fragments prepared corresponding to each sub-information recording base sequence are respectively inserted into different vector plasmids to obtain a recombinant plasmid set for recording information.

In another aspect, the present invention provides a method for DNA access of data information, the method comprising the following steps:

Step S1) using the above method to store data information in DNA,

Step S2) extracts the DNA and then performs analysis and decoding to obtain the stored data information. Step S2) includes the following steps.

Step S21): extracting the vector plasmid with the information recording fragment and performing sequencing reading;

Step S22): according to the sequencing data, the restriction sites and the junction arm sequences in the sequence are removed to obtain the base sequence that records the digital information;

Step S23): converting the base sequence information obtained in step S22) into binary information;

Step S24): extract corresponding data information from the binary information.

Another aspect of the present invention provides use of the above-mentioned recombinant plasmid or the above-mentioned recombinant plasmid library of the present invention in preparing a recombinant plasmid for use in DNA storage information.

Another aspect of the present invention provides a device for storing DNA data using a recombinant plasmid library containing universal information storage DNA, the device comprising:

A binary sequence extraction unit, used to extract a binary sequence corresponding to the data to be stored;

A binary sequence-base sequence conversion unit, used to convert the binary sequence into a base sequence, or convert the base sequence into a binary sequence according to a preset mapping relationship;

A short base fragment splitting design unit is used to split the base sequence into short base fragments, and adjacent short base fragments have overlapping base sequences; or

The short base segments composed of adjacent odd-numbered bit sequences and the short base segments composed of adjacent even-numbered bit sequences include overlapping base sequences;

The short base fragment splitting design unit can further split each of the short base fragments obtained by splitting from the middle, so that each short base fragment is split into a first short base fragment and a second short base fragment;

A recombinant plasmid library unit, wherein the recombinant plasmid library comprises the above-mentioned recombinant plasmid containing universal information storage DNA, and the recombinant plasmid library unit can also select all required recombinant plasmids for information recording and recombinant plasmids for vectors from the recombinant plasmids, wherein the first base linker of the required recombinant plasmid for information recording is identical to the first short base segment of the short base segment obtained by the short base segment splitting design unit, and the second base linker is identical to the second short base segment of the short base segment obtained by the short base segment splitting design unit; the first base linker on the recombinant plasmid for vectors is identical to the first short base segment of the 5' end short base segment of the base sequence obtained by the binary sequence-base sequence conversion unit, and the second base linker is identical to the second short base segment of the 3' end short base segment of the base segment of the binary sequence-base sequence conversion unit;

The recombinant plasmid library enzyme digestion and splicing unit is used to digest and connect all the required information recording recombinant plasmids selected by the recombinant plasmid library unit into information recording fragments, and can digest the vector recombinant plasmid and connect the information recording fragments to obtain the recombinant plasmid;

The storage and replication unit is used to store the recombinant plasmid obtained by the enzyme cutting and splicing unit of the recombinant plasmid library, or to replicate the recombinant plasmid of the recombinant plasmid library unit or the recombinant plasmid of the storage information obtained by the enzyme cutting and splicing unit of the recombinant plasmid library.

Furthermore, the device also includes a decoding device.

Furthermore, the decoding device comprises:

A DNA sequence acquisition unit, used to acquire the DNA sequence in the recombinant plasmid storing information;

The data analysis unit is used to remove the restriction sites and linker arm sequences in the DNA sequence to obtain the base sequence that records the digital information; and convert the base sequence into a binary sequence, and further convert it into storage data.

Still another aspect of the present invention provides a computer-readable storage medium having a computer program stored thereon, wherein when the computer program is executed by a processor, the steps of the above-mentioned DNA storage method or the steps of the above-mentioned DNA access method for data information are implemented.

Still another aspect of the present invention provides a computer device comprising a memory and a processor, wherein the memory stores a computer program that can be run on the processor, and when the processor executes the computer program, the steps of the above-mentioned DNA storage method or the steps of the above-mentioned DNA access method for data information are implemented.

Beneficial Effects

In view of the current DNA data storage technology, synthetic DNA cannot be reused and the cost of DNA data is high. The present invention provides a practical and feasible technical method to solve this problem.

The present invention creatively designs a recombinant plasmid library of universal information storage DNA, which contains a variety of recombinant plasmids. Each time it is used for information storage, only the required recombinant plasmids need to be selected. The consumed recombinant plasmids can be quickly replenished by bacterial fermentation, without the need to use chemical methods to construct each time.

The method of the present invention successfully realizes the assembly synthesis and in vivo and in vitro storage of any DNA by splitting the DNA sequence storing data into small overlapping sequence fragments and utilizing a pre-synthesized recombinant plasmid library that can be retrieved and used multiple times.

The method of the present invention is cleverly designed and can be applied to DNA data storage in different scenarios, and has the characteristics of high flexibility, good versatility and low cost. The present invention uses bacterial fermentation to prepare plasmid DNA in large quantities, which has low pollution and low cost compared to traditional chemically synthesized DNA. The method established by the present invention is not only suitable for storing data in freeze-dried plasmid DNA, but also suitable for storing data on a large scale in organisms, significantly reducing the cost of storing DNA in organisms.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG1 is a schematic diagram of a recombinant plasmid containing universal information storage DNA.

Among them, 1 and 5 are restriction endonuclease linkers, 2 is the first base linker, 3 is the linker arm sequence, and 4 is the second base linker.

FIG. 2 is a schematic diagram of staggered overlap of 3 bases or staggered overlap of 4 adjacent short base fragments.

FIG. 3 is a schematic diagram of the preparation of a recombinant plasmid of universal information storage DNA.

FIG. 4 is a schematic diagram showing the staggered overlapping of four bases in an odd-number plus an even-number overlapping manner.

FIG5 is a schematic diagram of base padding when the length of the split block of the last short base fragment cannot be consistent with the length of the previous short base fragment.

FIG. 6 is a schematic flow chart of a method for preparing a recombinant plasmid containing universal information storage DNA.

FIG. 7 is a schematic flow chart of the DNA data storage method of the present invention.

FIG. 8 is a schematic flow chart of the DNA data access method of the present invention.

Detailed ways

In order to make the above-mentioned objects, features and advantages of the present invention more obvious and understandable, the specific implementation modes of the present invention are described in detail below with reference to the accompanying drawings, but they should not be construed as limiting the applicable scope of the present invention.

In order to solve the problem of high storage and replication costs in the prior art methods of storing information in the form of DNA, a universal information storage plasmid DNA library that can be synthesized in advance and retrieved for multiple uses is proposed. The universal information storage plasmid DNA library can be mass-produced in advance or specifically synthesized according to the specific storage information.

As shown in FIG1 , the technical solution of the present invention first provides a method for preparing a recombinant plasmid containing universal information storage DNA, and the preparation method comprises the following steps:

S01): designing a universal information DNA sequence module, which comprises two sets of base linkers and a linker arm sequence located between the two sets of base linkers to obtain a base linker core sequence; adding a second type of restriction endonuclease linker to both ends of the base linker core sequence to obtain a universal information DNA sequence module sequence;

S02): synthesizing a universal information DNA sequence module and inserting it into a plasmid vector to obtain a recombinant plasmid;

Optionally, S03): the recombinant plasmid is introduced into a host bacterium for culture and preservation, or the recombinant plasmid containing the universal information DNA sequence is extracted from the host bacterium for preservation.

In the above preparation method S01), the base linker is a set formed by any combination of bases, and the base linker is used to record information.

At the same time, the base linker is also used for complementary connection with base linkers in other base linker core sequences.

Furthermore, the linker arm sequence is a known and determined base sequence.

The linker arm sequence is used to connect two sets of base linkers.

Furthermore, the two groups of base linkers have n1 bases and n2 bases respectively, and n1 and n2 are independently selected from any integer of 3-10, for example, independently selected from 3, 4, 5, 6, 7, 8, 9, 10.

The above-mentioned known and determined base sequence means that when the recombinant plasmid is synthesized, the linker arm sequence is an arbitrary determined base sequence, and the base sequence of the sequence can be determined according to actual needs, such as synthesis efficiency, etc. Theoretically, it can be any sequence, but since the sequence is removed in the later decoding process, the base sequence of the sequence is a known and determined base sequence during the synthesis process.

The above-mentioned base linkers are a set formed by any combination of bases, which means that during the synthesis process, the base linker sequence changes relatively. When preparing a complete set of plasmid libraries, base linkers with various arrangements and combinations need to be synthesized.

In the above preparation method S01), the type II restriction endonuclease linkers added to the left and right ends of the base linker core sequence can enable the sequence to obtain sticky ends having the above base linkers at both ends after being cut by the corresponding type II restriction endonuclease.

In a specific embodiment, the two groups of base adapters in step S01) are the first base adapter and the second base adapter, and the lengths of the first base adapter and the second base adapter are independently selected from 3-5. For example, the first base adapter is 4 bases and the second base adapter is 4 bases, thereby generating an abundance of 4 ⁸ = 65536 combinations. Or the first base adapter is 3 bases and the second base adapter is 3 bases, thereby generating an abundance of 4 ⁶ = 4096 combinations. The first base adapter is 3 bases and the second base adapter is 4 bases, or the second base adapter is 3 bases and the first base adapter is 4 bases, thereby generating an abundance of 4 ⁷ = 16384 combinations, and so on.

In a specific embodiment, the linker arm sequence in step S01) is a base sequence of fixed length. For example, the length of the first linker arm sequence is more than 3 bases, such as 3-100 bases, 10, 20, 30, 40, 50, 60, 70, 80, 90 bases.

In a specific embodiment, the restriction endonucleases added to the left and right ends of the base linker core sequence in step S01) are restriction endonucleases of the same type, or restriction endonucleases of different types, and the restriction endonucleases can enzymatically cut out sticky ends containing the first base linker and the second base linker.

In a specific embodiment, the restriction endonuclease can be selected from any one or more combinations of Alw I, AflII, AflIII, AgeI, ApaI, ApaLI, ApeKI, ApoI, AscI, AvaI, AvaII, BamHI, BanI, BanII, Bbs I, Bbv I, Bbv CI, BciV I, Bpm I, Bsal I, BsmA I, BsmF I, Eci I, Fok I, Hga I, Hph I, Mbo II, or Mme I. Alternatively, a restriction endonuclease known in the prior art that can produce a sticky end that can enzymatically cut out a first base linker and a second base linker can also be used.

In a specific implementation, in step S02), the synthesis method of the universal information DNA sequence module is chemical synthesis or biological synthesis.

On the basis of the above technical solution, the present invention also provides a recombinant plasmid containing universal information storage DNA prepared by the above preparation method.

On the basis of the above technical solution, the present invention also provides a recombinant plasmid library containing universal information storage DNA, wherein the recombinant plasmid library comprises two or more recombinant plasmids containing universal information storage DNA with different base linkers.

On the basis of the above technical solution, the recombinant plasmid library comprises 4 ^(n1+n2) types of recombinant plasmids containing universal information storage DNA with different base linkers, and n1 and n2 are the base lengths of the first base linker and the second base linker, respectively.

In some technical application scenarios, the recombinant plasmid or recombinant plasmid library for preparing universal information storage DNA of the present invention is reasonably preserved, and the cost of storing information with DNA can be reduced through large-scale preparation or prefabrication.

In a specific implementation, the synthesis method of the universal information DNA sequence module in step S02) is chemical synthesis or biological synthesis.

Some embodiments of the present invention also provide a DNA storage method for data, the DNA storage method comprising the following steps:

S11) extracting binary information of computer data information to be stored;

S12) converting the binary information into a base sequence for information recording according to a preset mapping relationship;

S13) splitting the information recording base sequence into short base fragments, wherein adjacent short base fragments have overlapping base sequences;

S14) splitting each of the short base fragments obtained by splitting in step S13) from the middle, so that each short base fragment is split into a first short base fragment and a second short base fragment;

S15) preparing by the preparation method of the present invention or selecting from the recombinant plasmid library containing universal information storage DNA of the present invention, obtaining the required recombinant plasmid with universal information DNA sequence for information recording; the first base linker on the recombinant plasmid with universal information DNA sequence for information recording is the same as the first short base fragment designed in step S14), and the second base linker is the same as the second short base fragment obtained in step S14); obtaining the recombinant plasmid with universal information DNA sequence corresponding to all the short base fragments split in step S13);

S16) using restriction endonucleases to digest the recombinant plasmid obtained in step S15), collecting the digested fragments, and connecting them into a complete information recording fragment using a ligase;

S17) preparing a recombinant plasmid with a universal information DNA sequence for a vector by the preparation method of the present invention or selecting from the recombinant plasmid library containing universal information storage DNA of the present invention; the first base linker on the recombinant plasmid with a universal information DNA sequence for a vector is the same as the first short base fragment of the 5' end short base fragment of the information recording base sequence designed in step S13), and the second base linker is the same as the second short base fragment of the 3' end short base fragment of the information recording base sequence designed in step S13);

S18) using restriction endonuclease to digest the recombinant plasmid obtained in step S17), collecting the digested plasmid as a vector plasmid, inserting the information recording fragment obtained in step S16) into the vector plasmid, and obtaining a recombinant plasmid for recording information.

Furthermore, the recombinant plasmid with recorded information obtained in step S18) is directly freeze-dried and stored in vitro.

Furthermore, the recombinant plasmid containing the recorded information obtained in step S18) is transferred into the host bacteria and replicated and stored.

Furthermore, the recombinant plasmid recording the information obtained in step S18) is transferred into the host bacteria, replicated, extracted and stored in the form of freeze-dried powder.

Furthermore, the host bacteria is any microorganism that can replicate and preserve the recombinant plasmid, and is more preferably Escherichia coli, yeast, lactobacillus, bifidobacterium, or enterococcus.

In a specific implementation, in step S11), the computer data information is selected from any data information that can exist on a computer, such as pictures, texts, programs, audio, and video.

In a specific implementation, in step S12), the binary information is information composed of 0 and 1, and the base sequence is a base sequence composed of four types of bases: A, T, C, and G. The mapping relationship includes any conversion method that can realize 0/1 to A/T/C/G information, such as Huffman coding published by Goldman et al. (Goldman et al. 2013, Nature), Fountain coding published by Erlich et al. (Erlich et al. 2017, Science), etc.

In a specific implementation, the mapping relationship is 00 is mapped to A, 01 is mapped to T, 11 is mapped to C, and 10 is mapped to G. The mapping relationship may also be a combination of different binary codes and base codes, for example, 11 is mapped to A, 10 is mapped to T, 01 is mapped to C, and 00 is mapped to G.

In a specific implementation, when the base sequence file for information recording is large, the base sequence for information recording can be split into multiple sub-files and recorded with index sequences; and in step S13), the corresponding index sequences are selected for splitting. Index sequences are, for example, AAAA=1, AAAG=2, AAAT=3, AAAC=4, AATA=5, AACA=6, etc.

In a specific embodiment, in step S13), the lengths of overlapping base sequences between adjacent short base fragments are equal or unequal, and the length of the overlapping base sequences is 1-10 bases in length, such as an overlap of 1 base, an overlap of 2 bases, an overlap of 3 bases, an overlap of 4 bases, an overlap of 5 bases, an overlap of 6 bases, an overlap of 7 bases, etc. As shown in FIG. 2 , an overlap of 3 bases and an overlap of 4 bases are shown.

In a specific embodiment, in step S13), there is no overlapping base sequence between two short base fragments separated by one short base fragment.

In a specific embodiment, the staggered overlapping manner between the short base fragments in step S13) can be a staggered overlapping of adjacent sequences, or can be another regular overlapping manner, such as an odd-number plus an even-number overlapping manner.

FIG. 2 shows the overlapping of adjacent sequences, and FIG. 4 shows the overlapping of odd-numbered bit sequences.

In a specific implementation, in step S13), when the length of the last short base segment cannot be consistent with the length of the previous short base segment, the segment is padded with bases and recorded. (Figure 5)

In a specific embodiment, the plasmid DNA in step S18) can be stored in a host bacterial solution in the form of a bacterial solution or extracted and freeze-dried into a dry powder.

In a specific embodiment, the plasmid DNA extracted in step S18) can be stored at different temperature conditions, including -20°C, -80°C, etc.

In some embodiments of the present invention, a method for decoding data information is also provided.

Step S21): extracting the vector plasmid with the information recording fragment and performing sequencing reading;

Step S22): according to the sequencing data, the restriction sites and the junction arm sequences in the sequence are removed to obtain the base sequence that records the digital information;

Step S23): converting the base sequence information obtained in step S22) into 0/1 binary information;

Step S24): extract corresponding picture/text/video information from the 0/1 binary information.

In a specific implementation, in step S21), the method of synthetic DNA sequencing reading includes any method that can read a DNA sequence library, such as second-generation sequencing, third-generation sequencing, etc.

In a specific implementation, when the sequencing data has an index sequence in step S22), the index sequence is read, and index sequences of different encoding orders are arranged, and the index sequences are removed and then assembled into a complete base sequence.

In a specific implementation, in step S22), the index sequence may group sequences belonging to the same file together.

In a specific implementation, in step S23), the conversion process of base sequence information to 0/1 binary information, the mapping relationship between A/T/C/G in the base sequence and the 0/1 of the binary information is the mapping relationship preset in step S12) of the DNA storage method of the above data.

In a specific implementation, in step S24), the method for extracting corresponding picture/text/video information from binary 0/1 information is any method.

In a specific embodiment, step S01) of the method for preparing a recombinant plasmid containing universal information storage DNA, steps S11-S14) of the method for storing data in DNA, and steps S22-S24) of the method for decoding data information can be implemented by manual calculation or by computer program.

Some embodiments of the present invention also provide a device for storing DNA data using a recombinant plasmid library containing universal information storage DNA, the device comprising:

A binary sequence extraction unit, used to extract a binary sequence corresponding to the data to be stored;

A binary sequence-base sequence conversion unit, used to convert the binary sequence into a base sequence, or convert the base sequence into a binary sequence according to a preset mapping relationship;

A short base fragment splitting design unit is used to split the base sequence into short base fragments, and adjacent short base fragments have overlapping base sequences; or

The short base segments composed of adjacent odd-numbered bit sequences and the short base segments composed of adjacent even-numbered bit sequences include overlapping base sequences;

The short base fragment splitting design unit can further split each of the short base fragments obtained by splitting from the middle, so that each short base fragment is split into a first short base fragment and a second short base fragment;

A recombinant plasmid library unit, wherein the recombinant plasmid library comprises the above-mentioned recombinant plasmid containing universal information storage DNA, and the recombinant plasmid library unit can also select all required recombinant plasmids for information recording and recombinant plasmids for vectors from the recombinant plasmids, wherein the first base linker of the required recombinant plasmid for information recording is identical to the first short base segment of the short base segment obtained by the short base segment splitting design unit, and the second base linker is identical to the second short base segment of the short base segment obtained by the short base segment splitting design unit; the first base linker on the recombinant plasmid for vectors is identical to the first short base segment of the 5' end short base segment of the base sequence obtained by the binary sequence-base sequence conversion unit, and the second base linker is identical to the second short base segment of the 3' end short base segment of the base segment of the binary sequence-base sequence conversion unit;

The recombinant plasmid library enzyme digestion and splicing unit is used to digest and connect all the required information recording recombinant plasmids selected by the recombinant plasmid library unit into information recording fragments, and can digest the vector recombinant plasmid and connect the information recording fragments to obtain the recombinant plasmid;

The storage and replication unit is used to store the recombinant plasmid obtained by the enzyme cutting and splicing unit of the recombinant plasmid library, or to replicate the recombinant plasmid of the recombinant plasmid library unit or the recombinant plasmid of the storage information obtained by the enzyme cutting and splicing unit of the recombinant plasmid library.

In some specific embodiments, the binary sequence-base sequence conversion unit is used to: encode each binary unit in the binary data using a different mapping coding rule, or encode two adjacent binary units in the binary data using different mapping coding rules.

In some specific embodiments, the device further includes a decoding device.

In some specific embodiments, the decoding device includes:

A DNA sequence acquisition unit, used to acquire the DNA sequence in the recombinant plasmid storing information;

The data analysis unit is used to remove the restriction sites and linker arm sequences in the DNA sequence to obtain the base sequence that records the digital information; and convert the base sequence into a binary sequence, and further convert it into storage data.

Some embodiments of the present invention further provide a computer-readable storage medium having a computer program stored thereon, wherein the computer program, when executed by a processor, implements the steps of the above-mentioned DNA storage method or the steps of the above-mentioned DNA access method for data information.

Some embodiments of the present invention also provide a computer device, including a memory and a processor, wherein the memory stores a computer program that can be run on the processor, and when the processor executes the computer program, the steps of the above-mentioned DNA storage method or the steps of the above-mentioned DNA access method for data information are implemented.

In a specific implementation manner, the process implemented by the above-mentioned computer program can be a computer program written in any language, such as Python, R, Perl, C++, etc.

The present invention may be a system, a method and/or a computer program product. The computer program product may include a computer-readable storage medium carrying computer-readable program instructions for causing a processor to implement various aspects of the present invention.

Computer readable storage medium can be a tangible device that can hold and store instructions used by an instruction execution device. Computer readable storage medium can be, for example, but not limited to, an electrical storage device, a magnetic storage device, an optical storage device, an electromagnetic storage device, a semiconductor storage device, or any suitable combination thereof. More specific examples (non-exhaustive list) of computer readable storage medium include: a portable computer disk, a hard disk, a random access memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or flash memory), a static random access memory (SRAM), a portable compact disk read-only memory (CD-ROM), a digital versatile disk (DVD), a memory stick, a floppy disk, a mechanical encoding device, for example, a punch card or a convex structure in a groove on which instructions are stored, and any suitable combination thereof. The computer readable storage medium used here is not interpreted as a transient signal itself, such as a radio wave or other freely propagating electromagnetic wave, an electromagnetic wave propagated by a waveguide or other transmission medium (for example, a light pulse by an optical fiber cable), or an electrical signal transmitted by a wire.

The computer-readable program instructions described herein can be downloaded from a computer-readable storage medium to each computing/processing device, or downloaded to an external computer or external storage device via a network, such as the Internet, a local area network, a wide area network, and/or a wireless network. The network can include copper transmission cables, optical fiber transmissions, wireless transmissions, routers, firewalls, switches, gateway computers, and/or edge servers. The network adapter card or network interface in each computing/processing device receives the computer-readable program instructions from the network and forwards the computer-readable program instructions for storage in the computer-readable storage medium in each computing/processing device.

The computer program instructions for performing the operation of the present invention may be assembly instructions, instruction set architecture (ISA) instructions, machine instructions, machine-dependent instructions, microcode, firmware instructions, state setting data, or source code or object code written in any combination of one or more programming languages, including object-oriented programming languages, such as Smalltalk, C++, Python, etc., and conventional procedural programming languages, such as "C" language or similar programming languages. Computer-readable program instructions may be executed entirely on a user's computer, partially on a user's computer, as an independent software package, partially on a user's computer, partially on a remote computer, or entirely on a remote computer or server. In the case of a remote computer, the remote computer may be connected to the user's computer via any type of network, including a local area network (LAN) or a wide area network (WAN), or may be connected to an external computer (e.g., using an Internet service provider to connect via the Internet). In some embodiments, an electronic circuit, such as a programmable logic circuit, a field programmable gate array (FPGA), or a programmable logic array (PLA), may be personalized by utilizing the state information of the computer-readable program instructions, and the electronic circuit may execute the computer-readable program instructions, thereby realizing various aspects of the present invention.

Various aspects of the present invention are described herein with reference to the flow charts and/or block diagrams of the methods, devices (systems) and computer program products according to embodiments of the present invention. It should be understood that each box of the flow chart and/or block diagram and the combination of each box in the flow chart and/or block diagram can be implemented by computer-readable program instructions.

These computer-readable program instructions can be provided to a processor of a general-purpose computer, a special-purpose computer, or other programmable data processing device, thereby producing a machine, so that when these instructions are executed by the processor of the computer or other programmable data processing device, a device that implements the functions/actions specified in one or more boxes in the flowchart and/or block diagram is generated. These computer-readable program instructions can also be stored in a computer-readable storage medium, and these instructions cause the computer, programmable data processing device, and/or other equipment to work in a specific manner, so that the computer-readable medium storing the instructions includes a manufactured product, which includes instructions for implementing various aspects of the functions/actions specified in one or more boxes in the flowchart and/or block diagram.

Computer-readable program instructions may also be loaded onto a computer, other programmable data processing apparatus, or other device so that a series of operating steps are performed on the computer, other programmable data processing apparatus, or other device to produce a computer-implemented process, thereby causing the instructions executed on the computer, other programmable data processing apparatus, or other device to implement the functions/actions specified in one or more boxes in the flowchart and/or block diagram.

Example 1 Construction of a recombinant plasmid library containing universal information storage DNA

In this embodiment, a recombinant plasmid library with a first base sequence and a second base sequence of 4 bases in length is constructed. Recombinant plasmid libraries with other base lengths can also be obtained by a similar method.

01. Design a universal information DNA sequence module, which includes two groups of base linkers and a linker arm sequence GGATCTCA located between the two groups of base linkers to obtain a base linker core sequence; add a second type of restriction endonuclease linker to the left and right ends of the base linker core sequence to obtain a universal information DNA sequence module sequence; the second type of restriction endonuclease linker selected in this embodiment is the BsalI enzyme digestion sequence:

5'-GGTCTC(N) ₁ 3' SEQ ID NO.1

3'-CCAGAG(N) ₅ 5'. SEQ ID NO.2

The two groups of base linkers are respectively called the first base linker and the second base linker, and the first base linker is defined as being 5' upstream of the second base linker. The length of the two groups of base linkers is 4 bases, and different permutations and combinations are performed to obtain 4 ⁸ combinations.

02. Synthesize the universal information DNA sequence modules designed and obtained in step 01 respectively and insert them into plasmid vectors to obtain recombinant plasmids;

03. Introduce the recombinant plasmid into Escherichia coli for cultivation, and prepare and extract the recombinant plasmid containing the universal information DNA sequence.

04. Collect the recombinant plasmids containing different first base linkers and second base linkers to form a recombinant plasmid library containing universal information storage DNA.

Example 2: Storing and decoding data information

1. Use a computer program to extract the binary code of "China" GB18030: "11010110110100001011100111111010".

2. Using the coding rule "A=00, T=01, C=11, G=10", the above binary information is converted into DNA sequence information "GTTCGTAACGCTGGCC" SEQ ID NO.3.

3. Divide the DNA sequence information into three short base fragments: 1) "GTTCGTAA" SEQ ID NO. 4, 2) "GTAACGCT" SEQ ID NO. 5, and 3) "CGCTGGCC" SEQ ID NO. 6. Adjacent fragments have overlapping base sequences GTAA and CGCT.

4. Split the above short base fragment into two segments, as shown below: "GTTC/GTAA", "GTAA/CGCT", "CGCT/GGCC" sequences.

5. Find a plasmid having a first base sequence and a second base sequence corresponding to the short base fragment in step 4, wherein the plasmid has the following double-stranded sequence:

1) "GGTCTCA GTTC GGATCTCA GTAA AGAGACC SEQ ID NO.7

CCAGAGTCAAGCCTAGAGTCATTTCTCTGG" SEQ ID NO.8;

2) "GGTCTCA GTAA GGATCTCA CGCT AGAGACC SEQ ID NO.9

CCAGAGTCATTCCTAGAGTGCGATCTCTGG" SEQ ID NO.10;

3) "GGTCTCA CGCT GGATCTCA GGCC AGAGACC SEQ ID NO.11

CCAGAGTGCGACCTAGAGTCCGGTCTCTGG" SEQ ID NO.12.

The restriction endonuclease cleavage site added in this example is the BsalI cleavage site:

5'-GGTCTC(N) ₁ -3'

3'-CCAGAG(N) ₅ -5'

The linker spacer sequence was GGATCTCA.

100 ng of plasmids containing the above sequences were taken from the plasmid library of Example 1, and digested with BsalI to obtain fragments.

1)“ GTTC GGATCTCASEQ ID NO.13

CCTAGAGTCATT" SEQ ID NO.14;

2) " GTAA GGATCTCA SEQ ID NO.15

CCTAGAGTGCGA" SEQ ID NO.16;

3) " CGCT GGATCTCA SEQ ID NO.17

CCTAGAGTCCGG". SEQ ID NO.18

The three fragments are connected by ligase to form a complete information recording fragment

GTTC GGATCTCA GTAA GGATCTCA CGCT GGATCTCA SEQ ID NO.19

CCTAGAGTCATT CCTAGAGTGCGACCTAGAGTCCGG. SEQ ID NO.20

5. Take out a plasmid with the same first base sequence at the 5' end of the first fragment and the same second base sequence at the 3' end of the last fragment from the plasmid library of Example 1, that is, a plasmid with a first base sequence of GTTC and a second base sequence of GGCC, and digest it with enzymes to obtain an empty vector.

6. Insert the complete information recording fragment obtained in step 4 into the empty vector in step 5 and transform it into Escherichia coli.

7. Store E. coli on the relevant plasmid vector in the form of glycerol tubes.

Data interpretation

8. Extract the plasmid in step 7 above and send it to a next-generation sequencing service company for sequencing to obtain the following general sequence information in the middle of the restriction site:

"GGTCTCA GTTC GGATCTCAGTAAAGAGACC SEQ ID NO.7

CCAGAGTCAAGCCTAGAGTCATTTCTCTGG" SEQ ID NO.8;

"GGTCTCAGTAAGGATCTCACGCTAGAGACC SEQ ID NO.9

CCAGAGTCATTCCTAGAGTGCGATCTCTGG" SEQ ID NO.10

"GGTCTCACGCTGGATCTCAGGCCAGAGACC SEQ ID NO.11

CCAGAGTGCGACCTAGAGTCCGGTCTCTGG" SEQ ID NO.12

9. Remove the linker arm sequence in the sequence to obtain the left and right linkers "GTTC GTAA", "GTAA CGCT" and "CGCTGGCC".

10. Assemble the complete DNA sequence GTTCGTAACGCTGGCC from the left and right linker sequences. SEQ ID NO.3

11. Use the coding rule “A=00, T=01, C=11, G=10” to convert the DNA sequence into “11010110110100001011100111111010”.

12. Extract “China” from the binary sequence.

The implementation effect of this simulation embodiment is that in the above embodiment, a small amount of DNA was taken out from the prepared universal DNA library for DNA storage of "China" information. Compared with the traditional DNA storage technology, the reuse of synthesized DNA is effectively realized. Specifically in this embodiment, a small amount of prepared DNA is used to reduce the storage cost of this section of information compared to traditional technology storage. In the specific experiment, the amount of plasmid DNA taken out at a time can even reach the single-molecule level, which enables the cost of implementing the technical method to be further significantly reduced. At the same time, due to the large-scale preparation of plasmid DNA, the cost will be further reduced, and the price of DNA storage achieved using this process can be further reduced.

SEQUENCE LISTING

<110> Shenzhen Institute of Advanced Technology

<120> Data information storage method based on DNA molecule medium

<130> CP122010180E

<160> 20

<170> PatentIn version 3.3

<210> 1

<211> 6

<212> DNA

<213> Artificial sequence

<400> 1

ggtctc 6

<210> 2

<211> 6

<212> DNA

<213> Artificial sequence

<400> 2

ccagag 6

<210> 3

<211> 16

<212> DNA

<213> Artificial sequence

<400> 3

gttcgtaacg ctggcc 16

<210> 4

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<212> DNA

<213> Artificial sequence

<400> 4

gttcgtaa 8

<210> 5

<211> 8

<212> DNA

<213> Artificial sequence

<400> 5

gtaacgct 8

<210> 6

<211> 8

<212> DNA

<213> Artificial sequence

<400> 6

cgctggcc 8

<210> 7

<211> 30

<212> DNA

<213> Artificial sequence

<400> 7

ggtctcagtt cggatctcag taaagagacc 30

<210> 8

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<212> DNA

<213> Artificial sequence

<400> 8

ccagagtcaa gcctagagtc atttctctgg 30

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<211> 30

<212> DNA

<213> Artificial sequence

<400> 9

ggtctcagta aggatctcac gctagacc 30

<210> 10

<211> 30

<212> DNA

<213> Artificial sequence

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ccagagtcat tcctagagtg cgatctctgg 30

<210> 11

<211> 30

<212> DNA

<213> Artificial sequence

<400> 11

ggtctcacgc tggatctcag gccagagacc 30

<210> 12

<211> 30

<212> DNA

<213> Artificial sequence

<400> 12

ccagagtgcg acctagagtc cggtctctgg 30

<210> 13

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<212> DNA

<213> Artificial sequence

<400> 13

gttcggatct ca 12

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<212> DNA

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cctagagtca tt 12

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<212> DNA

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gtaaggatct ca 12

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<212> DNA

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cctagagtgc ga 12

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<212> DNA

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<400> 17

cgctggatct ca 12

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<212> DNA

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cctagagtcc gg 12

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<212> DNA

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gttcggatct cagtaaggat ctcacgctgg atctca 36

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<212> DNA

<213> Artificial sequence

<400> 20

cctagagtca ttcctagagt gcgacctaga gtccgg 36

Claims (15)

1. A method for preparing a recombinant plasmid containing universal information storage DNA, characterized in that the preparation method comprises the following steps: S01) Designing a universal information DNA sequence module, which comprises two groups of base linkers and a linker arm sequence between the two groups of base linkers, connecting the two groups of base linkers and the linker arm sequence between the two groups of base linkers to obtain a base linker core sequence; adding two types of restriction endonuclease linker sequences to the left and right ends of the base linker core sequence to obtain a universal information DNA sequence module sequence; the base linker is a set formed by any combination of bases, and the base linker is used to record information; the linker arm sequence is a known and determined base sequence; the linker arm sequence of the recombinant plasmid in the same recombinant plasmid library is the same sequence; S02) synthesizing a universal information DNA sequence module and inserting it into a plasmid vector to obtain a recombinant plasmid; S03) introducing the recombinant plasmid into a host bacterium for culture and preservation, or extracting the recombinant plasmid containing the universal information DNA sequence from the host bacterium for preservation. 2. A recombinant plasmid containing universal information storage DNA, characterized in that the recombinant plasmid is prepared by the preparation method described in claim 1. 3. A recombinant plasmid library, characterized in that the recombinant plasmid library comprises a plurality of recombinant plasmids containing universal information storage DNA according to claim 2; The recombinant plasmid library contains 4 ^(n1+n2) types of recombinant plasmids containing universal information storage DNA with different base linkers, n1 and n2 are respectively the base lengths of the first base linker and the second base linker in the two groups of base linkers; n1 and n2 are independently selected from any integers of 3-10. 4. A DNA storage method for data, characterized in that the DNA storage method comprises the following steps: S11) extracting binary information of computer data information to be stored; S12) converting the binary information into a base sequence for information recording according to a preset mapping relationship; S13) splitting the information recording base sequence into short base segments, and providing overlapping base sequences between adjacent short base segments; or, There are also overlapping base sequences between the short base segments composed of adjacent odd-numbered bit sequences and between the short base segments composed of adjacent even-numbered bit sequences; S14) splitting each of the short base fragments obtained by splitting in step S13) from the middle, so that each short base fragment is split into a first short base fragment and a second short base fragment; S15) preparing by the preparation method described in claim 1, obtaining the required recombinant plasmid with universal information DNA sequence for information recording; the first base linker on the recombinant plasmid with universal information DNA sequence for information recording is the same as the first short base fragment designed in step S14), and the second base linker is the same as the second short base fragment obtained in step S14); obtaining the recombinant plasmid with universal information DNA sequence corresponding to all the short base fragments split in step S13); S16) using restriction endonucleases to digest the recombinant plasmid obtained in step S15), collecting the digested fragments, and connecting them into a complete information recording fragment using a ligase; S17) preparing by the preparation method of claim 1, obtaining a recombinant plasmid having a universal information DNA sequence for a vector; the first base linker on the recombinant plasmid having a universal information DNA sequence for a vector is the same as the first short base fragment of the 5' end short base fragment of the information recording base sequence designed in step S13), and the second base linker is the same as the second short base fragment of the 3' end short base fragment of the information recording base sequence designed in step S13); S18) using restriction endonucleases to digest the recombinant plasmid obtained in step S17), collecting the digested plasmid as a vector plasmid, inserting the information recording fragment obtained in step S16) into the vector plasmid, and obtaining a recombinant plasmid for recording information; In step S11), the computer data information can be data information existing on the computer, selected from pictures, texts, programs, audios, and videos. 5. A DNA storage method for data, characterized in that the DNA storage method comprises the following steps: S11) extracting binary information of computer data information to be stored; S12) converting the binary information into a base sequence for information recording according to a preset mapping relationship; S13) splitting the information recording base sequence into short base segments, and providing overlapping base sequences between adjacent short base segments; or, There are also overlapping base sequences between the short base segments composed of adjacent odd-numbered bit sequences and between the short base segments composed of adjacent even-numbered bit sequences; S14) splitting each of the short base fragments obtained by splitting in step S13) from the middle, so that each short base fragment is split into a first short base fragment and a second short base fragment; S15) selecting from the recombinant plasmid library of claim 3 to obtain the required recombinant plasmid with universal information DNA sequence for information recording; the first base linker on the recombinant plasmid with universal information DNA sequence for information recording is the same as the first short base fragment designed in step S14), and the second base linker is the same as the second short base fragment obtained in step S14); obtaining the recombinant plasmid with universal information DNA sequence corresponding to all the short base fragments split in step S13); S16) using restriction endonucleases to digest the recombinant plasmid obtained in step S15), collecting the digested fragments, and connecting them into a complete information recording fragment using a ligase; S17) selecting from the recombinant plasmid library of claim 3 to obtain a recombinant plasmid with a universal information DNA sequence for a vector; the first base linker on the recombinant plasmid with a universal information DNA sequence for a vector is the same as the first short base fragment of the 5' end short base fragment of the information recording base sequence designed in step S13), and the second base linker is the same as the second short base fragment of the 3' end short base fragment of the information recording base sequence designed in step S13); S18) using restriction endonucleases to digest the recombinant plasmid obtained in step S17), collecting the digested plasmid as a vector plasmid, inserting the information recording fragment obtained in step S16) into the vector plasmid, and obtaining a recombinant plasmid for recording information; In step S11), the computer data information can be data information existing on the computer, selected from pictures, texts, programs, audios, and videos. 6. The DNA storage method according to claim 4 or 5, characterized in that the recombinant plasmid recording information obtained in step S18) is directly freeze-dried and stored in vitro. 7. The DNA storage method according to claim 4 or 5 is characterized in that the recombinant plasmid recording the information obtained in step S18) is transferred into a host bacterium for replication and preservation; the host bacterium is any microorganism that can replicate and preserve the recombinant plasmid. 8. The DNA storage method according to claim 4 or 5 is characterized in that the recombinant plasmid recording information obtained in step S18) is transferred into a host bacterium, replicated, and then extracted and stored in the form of freeze-dried powder; the host bacterium is any microorganism that can replicate and store the recombinant plasmid. 9. The DNA storage method according to claim 4 or 5 is characterized in that, in step S13), when the base sequence is divided into multiple short base fragments, when the sequence length of the terminal short base fragment is smaller than that of other short base fragments, repeated bases are used to pad it to the same length as other short base fragments. 10. The DNA storage method according to claim 4 or 5, characterized in that, in step S12), before splitting the base sequence for information recording into short base fragments, the method further comprises splitting the base sequence for information recording into a plurality of sub-base sequences for information recording, and adding an index fragment before each sub-base sequence for information recording; The index fragment sequence length is 3-100 bases; The index fragment added before each fragment is different, and in S13), the sub-information records connected with different index fragments are split into short base fragments respectively using base sequences. 11. The DNA storage method according to claim 10, characterized in that, after the information recording base sequence is split into sub-information recording base sequences, in S16), a complete information recording fragment is prepared corresponding to each sub-information recording base sequence; After the information recording base sequence is split into sub-information recording base sequences, in S18), the information recording fragments prepared corresponding to each sub-information recording base sequence are respectively inserted into different vector plasmids to obtain a recombinant plasmid set for recording information. 12. A method for DNA access of data information, characterized in that the method comprises the following steps: Step S1) using the DNA storage method of claim 4 or 5 to store data information in DNA, Step S2) extracts the DNA and then analyzes and decodes it to obtain the stored data information. Step S2) includes the following steps: Step S21): extracting the vector plasmid with the information recording fragment and performing sequencing reading; Step S22): according to the sequencing data, the restriction sites and the junction arm sequences in the sequence are removed to obtain the base sequence that records the digital information; Step S23): converting the base sequence information obtained in step S22) into binary information; Step S24): extract corresponding data information from the binary information. 13. Use of the recombinant plasmid according to claim 2 in preparing a recombinant plasmid for use in DNA storage information. 14. Use of the recombinant plasmid library according to claim 3 in preparing recombinant plasmids for use in DNA storage information. 15. A device for storing DNA data using a recombinant plasmid library containing universal information storage DNA, characterized in that the device comprises: A binary sequence extraction unit, used to extract a binary sequence corresponding to the data to be stored; A binary sequence-base sequence conversion unit, used to convert the binary sequence into a base sequence, or convert the base sequence into a binary sequence according to a preset mapping relationship; A short base fragment splitting design unit is used to split the base sequence into short base fragments, and adjacent short base fragments have overlapping base sequences; or The short base segments composed of adjacent odd-numbered bit sequences and the short base segments composed of adjacent even-numbered bit sequences include overlapping base sequences; The short base fragment splitting design unit can further split each of the short base fragments obtained by splitting from the middle, so that each short base fragment is split into a first short base fragment and a second short base fragment; A recombinant plasmid library unit, wherein the recombinant plasmid library comprises the recombinant plasmid containing the universal information storage DNA according to claim 2, and the recombinant plasmid library unit can also select all required recombinant plasmids for information recording and recombinant plasmids for vectors from the recombinant plasmids, wherein the first base linker of the required recombinant plasmid for information recording is identical to the first short base fragment of the short base fragment obtained by the short base fragment splitting design unit, and the second base linker is identical to the second short base fragment of the short base fragment obtained by the short base fragment splitting design unit; the first base linker on the recombinant plasmid for vectors is identical to the first short base fragment of the 5' end short base fragment of the base sequence obtained by the binary sequence-base sequence conversion unit, and the second base linker is identical to the second short base fragment of the 3' end short base fragment of the base fragment of the binary sequence-base sequence conversion unit; The recombinant plasmid library enzyme digestion and splicing unit is used to digest and connect all the required information recording recombinant plasmids selected by the recombinant plasmid library unit into information recording fragments, and can digest the vector recombinant plasmid and connect the information recording fragments to obtain the recombinant plasmid; A storage and replication unit, used for storing the recombinant plasmid obtained by the enzyme digestion and splicing unit of the recombinant plasmid library, or for replicating the recombinant plasmid of the recombinant plasmid library unit or the recombinant plasmid of the storage information obtained by the enzyme digestion and splicing unit of the recombinant plasmid library; The device also includes a decoding device; The decoding device comprises: A DNA sequence acquisition unit, used to acquire the DNA sequence in the recombinant plasmid storing information; The data analysis unit is used to remove the restriction site and the linker arm sequence in the DNA sequence to obtain a base sequence that records digital information; and convert the base sequence into a binary sequence, and further convert it into storage data; wherein the linker arm sequence is a known and determined base sequence; and the recombinant plasmid linker arm sequences in the same recombinant plasmid library are the same sequence.