[go: up one dir, main page]

CN114947118B - Fermented dried date cheese and preparation method thereof - Google Patents

Fermented dried date cheese and preparation method thereof Download PDF

Info

Publication number
CN114947118B
CN114947118B CN202210392434.5A CN202210392434A CN114947118B CN 114947118 B CN114947118 B CN 114947118B CN 202210392434 A CN202210392434 A CN 202210392434A CN 114947118 B CN114947118 B CN 114947118B
Authority
CN
China
Prior art keywords
fermentation
jujube
cheese
dried
total
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202210392434.5A
Other languages
Chinese (zh)
Other versions
CN114947118A (en
Inventor
李焕荣
钟佳敏
陈恺
许铭强
陈文霞
马蕾
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xinjiang Fruit Industry Group Co ltd
Xinjiang Agricultural University
Original Assignee
Xinjiang Fruit Industry Group Co ltd
Xinjiang Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Xinjiang Fruit Industry Group Co ltd, Xinjiang Agricultural University filed Critical Xinjiang Fruit Industry Group Co ltd
Priority to CN202210392434.5A priority Critical patent/CN114947118B/en
Publication of CN114947118A publication Critical patent/CN114947118A/en
Application granted granted Critical
Publication of CN114947118B publication Critical patent/CN114947118B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/065Microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/90Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Dairy Products (AREA)

Abstract

本发明公开了一种发酵新产品枣酪干及其制备方法,该新产品直接采用鲜枣为原料,预处理后按照一定接种量接种悬菌液发酵,经烘制杀菌最终获得一种全新的发酵产品,该枣酪干制备工艺简单易操作,便于规模化生产,大大降低了生产成本,产品较干枣水分含量高、风味独特,色泽红润,解决了现有加工工艺造成枣果干制品品质不佳,颜色褐变和风味劣变的技术问题,实现了以鲜枣为原料加工过程的品质调控,且经过发酵处理降低了产品的含糖量同时形成了制品特殊的风味,当采用酵母菌‑乳酸菌按照质量比1:1的混合菌发酵,发酵过程的总糖含量最低为25.73%,总酚含量最高为47.83mg/g,本发明提供的枣酪干为一种全新的休闲食品且更符合健康理念。

The invention discloses a new fermented product, dried date cheese, and a preparation method thereof. The new product directly uses fresh dates as raw materials. After pretreatment, it is inoculated with suspension bacteria according to a certain inoculation amount and fermented. After baking and sterilization, a brand new product is finally obtained. Fermented products, the preparation process of dried jujube cheese is simple and easy to operate, convenient for large-scale production, and greatly reduces production costs. The product has a higher moisture content than dry jujubes, unique flavor, and ruddy color. It solves the problem of the quality of dried jujube products caused by the existing processing technology. Technical problems such as color browning and flavor deterioration are not good, and the quality control of the processing process using fresh dates as raw materials has been achieved, and the sugar content of the product has been reduced through fermentation treatment while forming a special flavor of the product. When using yeast - Lactic acid bacteria are fermented according to the mixed bacteria with a mass ratio of 1:1. The total sugar content during the fermentation process is at least 25.73% and the total phenolic content is at the highest 47.83 mg/g. The date cheese dried provided by the invention is a brand-new snack food and more In line with the health concept.

Description

一种发酵枣酪干及其制备方法A kind of fermented date cheese and preparation method thereof

技术领域Technical field

本发明涉及食品加工领域,特别涉及一种发酵新产品枣酪干及其制备方法。The invention relates to the field of food processing, and in particular to a new fermented date cheese product and a preparation method thereof.

技术背景technical background

红枣(Zizyphus jujube Mill.)是鼠李科(Rhamnaceae)枣属(Zizyphus Mill.)植物所结的果实。红枣是中国特有的经济果品,栽培面积约100hm2,年产量约400万吨。我国新鲜红枣目前主要的加工方式是干制,70-80%出口的红枣产品属于干制产品。目前我国市场上传统的红枣加工产品包括干制品枣泥、枣粉、枣蜜饯、乌枣、枣片、枣夹果仁、功能性产品如枣多糖、色素、膳食纤维等产品,还是软饮料类枣酒、枣醋、枣汁和枣酪等产品,这些加工产品均以干枣为原料进行二次加工,干枣原料主要以自然干制和人工干制为主,研究表明,干制后香气成分的种类和含量变化更加明显,但是其中的果胶、总酸、还原糖、总糖含量均有所下降;且在干制过程中加工环境的影响发生不同程度的品质劣,如色泽褐变变、营养成分损失、风味劣变(苦辣味)等,可见,干枣的品质直接影响成品的品质,导致加工产品的品质提升及创新空间较小,导致红枣加工产品特别是作为休闲食品的市场占有率有限。Zizyphus jujube Mill. is the fruit of the Zizyphus Mill. plant in the Rhamnaceae family. Red dates are a unique economic fruit in China, with a cultivation area of about 100hm2 and an annual output of about 4 million tons. The current main processing method of fresh red dates in my country is drying, and 70-80% of exported red dates are dried products. At present, traditional red date processed products on the Chinese market include dried products such as date puree, date powder, date preserves, black dates, date slices, dates with nuts, functional products such as date polysaccharides, pigments, dietary fiber and other products, as well as soft drink date wine. , date vinegar, date juice, date cheese and other products. These processed products use dried dates as raw materials for secondary processing. The raw materials of dried dates are mainly natural drying and artificial drying. Studies have shown that the aroma components of dried dates change. The changes in type and content are more obvious, but the contents of pectin, total acid, reducing sugar, and total sugar have all decreased; and the influence of the processing environment during the drying process has led to varying degrees of poor quality, such as browning of color, Loss of nutrients, deterioration of flavor (bitter and spicy taste), etc. It can be seen that the quality of dried dates directly affects the quality of finished products, resulting in less room for quality improvement and innovation of processed products, resulting in the market share of processed red date products, especially as snack foods. Rates are limited.

为丰富枣加工产品的种类及产品的创新,亟需开发更多的鲜枣直接加工产品,不仅减少了现有二次加工成本,且进一步改善现有干枣深加工中存在的褐变、营养成分损失、风味变差及含糖量高的问题。In order to enrich the types of jujube processed products and innovate products, it is urgent to develop more directly processed fresh jujube products, which not only reduces the existing secondary processing costs, but also further improves the browning and nutritional components existing in the deep processing of existing dried jujubes. Problems with loss, loss of flavor and high sugar content.

发明内容Contents of the invention

针对现有红枣深加工产品需要二次加工、加工工艺复杂成本高以及现有干枣深加工产品存在的褐变、营养成分损失、风味变差及含糖量高的技术现状。本发明旨在于提供一种发酵枣酪干及其制备方法,该新产品通过直接采用鲜枣为原料,预处理后按照一定接种量接种悬菌液发酵,经烤制杀菌最终获得一种全新的发酵产品,该枣酪干制备工艺简单易操作,便于规模化生产,大大降低了生产成本,产品较干枣水分含量高、风味独特,色泽红润,解决了现有加工工艺造成枣果干制品品质不佳,颜色褐变和风味劣变的技术问题,实现了以鲜枣为原料加工过程的品质调控,且经过发酵处理降低了产品的含糖量同时形成了制品特殊的风味,当采用酵母菌-乳酸菌按照质量比1:1的混合菌发酵,发酵过程中的总糖含量最低为25.73%,总酚含量最高为47.83mg/g,本发明提供的枣酪干为一种全新的休闲食品且更符合健康理念。In view of the technical status quo of existing deep-processed red dates products that require secondary processing, complex processing techniques and high costs, and existing deep-processed dried dates products that suffer from browning, loss of nutrients, deterioration of flavor and high sugar content. The present invention aims to provide a fermented date cheese dried product and a preparation method thereof. The new product is made by directly using fresh dates as raw materials, pre-processing and inoculating suspension fermentation according to a certain inoculation amount, and finally obtaining a brand-new product through baking and sterilization. Fermented product, the preparation process of this dried jujube cheese is simple and easy to operate, convenient for large-scale production, and greatly reduces production costs. The product has a higher moisture content than dry jujubes, unique flavor, and ruddy color. It solves the problem of the quality of dried jujube products caused by the existing processing technology. Technical problems such as color browning and flavor deterioration are not good, and the quality control of the processing process using fresh dates as raw materials has been achieved, and the sugar content of the product has been reduced through fermentation treatment while forming a special flavor of the product. When using yeast - Lactic acid bacteria are fermented according to the mixed bacteria with a mass ratio of 1:1. The total sugar content during the fermentation process is at least 25.73% and the total phenolic content is at the highest 47.83 mg/g. The date cheese provided by the invention is a brand-new snack food and More in line with the health concept.

本发明提供一种发酵枣酪干,该枣酪干通过新鲜枣果接种混合菌发酵烘干后获得。The invention provides a fermented date cheese, which is obtained by inoculating fresh date fruits with mixed bacteria, fermentation and drying.

本发明所述发酵枣酪干中,枣果为酸枣、骏枣、金丝枣中的任意一种。In the fermented jujube dried cheese of the present invention, the jujube fruit is any one of wild jujube, jujube, and golden jujube.

本发明所述发酵枣酪干中,该枣酪干采用如下步骤制备:新鲜枣果清洗预处理后,加入浓度为0.5%的柠檬酸护色处理10min;将处理后的枣果装入15×25cm真空袋,按照占枣果重量4%接种悬菌液封口,于37±1℃发酵60-84小时;将发酵后的枣果于55-60℃烘制10-12小时至水分含量为20%,真空包装常压水浴于80℃杀菌20min即得新产品枣酪干。In the fermented dried date cheese of the present invention, the dried date cheese is prepared by the following steps: after the fresh date fruit is washed and pre-treated, citric acid with a concentration of 0.5% is added for color protection treatment for 10 minutes; the processed date fruit is packed into 15× 25cm vacuum bag, inoculate 4% of the weight of the jujube fruit with a suspension, seal, and ferment at 37±1℃ for 60-84 hours; bake the fermented jujube fruit at 55-60℃ for 10-12 hours until the moisture content is 20 %, vacuum package and sterilize in a normal pressure water bath at 80°C for 20 minutes to obtain a new product, dried date cheese.

本发明提供的发酵枣酪干制备中,所述鲜枣预处理方式为去皮去核不刺孔、不去皮去核刺孔和不去皮去核不刺孔中的任意一种。In the preparation of fermented jujube dried cheese provided by the invention, the fresh jujube pretreatment method is any one of peeling and cored without puncturing, non-peeling, cored and punctured, and non-peeling, cored and punctured.

本发明提供的发酵枣酪干制备中,发酵方式为自然发酵、酵母菌发酵、混合菌发酵、乳酸菌发酵中的任意一种。In the preparation of fermented date cheese provided by the present invention, the fermentation method is any one of natural fermentation, yeast fermentation, mixed bacteria fermentation, and lactic acid bacteria fermentation.

本发明提供的发酵枣酪干制备中,采用不去皮去核不刺孔的鲜枣预处理方式,采用混合菌发酵,所述混合菌悬菌液按照如下步骤获得:酵母菌:乳酸菌按照质量比1:1溶于30±0.5℃温水中,制成浓度为20%的悬菌液,活化30min。In the preparation of fermented jujube dried cheese provided by the invention, fresh jujubes without peeling, pitting or puncturing are used for pretreatment, and mixed bacteria are used for fermentation. The mixed bacteria suspension is obtained according to the following steps: yeast: lactic acid bacteria according to the quality Dissolve in warm water at 30±0.5℃ with a ratio of 1:1 to make a suspension with a concentration of 20%, and activate it for 30 minutes.

采用上述技术方案后,本发明获得的有益效果如下:After adopting the above technical solution, the beneficial effects obtained by the present invention are as follows:

(1)利用本发明提供的方法获得的发酵新产品枣酪干,制备工艺简单易操作,便于规模化生产,大大降低了生产成本,产品较干枣水分含量高、风味独特,色泽红润,解决了现有加工工艺造成枣果干制品品质不佳,颜色褐变和风味劣变的技术问题。(1) The preparation process of the new fermented jujube cheese dried product obtained by the method provided by the invention is simple and easy to operate, convenient for large-scale production, and greatly reduces the production cost. The product has a higher moisture content than dry jujubes, unique flavor, and ruddy color, and solves the problem It solves the technical problems of poor quality, browning of color and deterioration of flavor caused by the existing processing technology of dried date fruit products.

(2)本发明提供的发酵新产品枣酪干,营养全面,总糖含量较鲜果下降但还原糖含量上升,总酸含量上升,总酚含量增加,新产品的制备解决了现有干枣制品因含糖量过高、含酸量不足造成口感偏甜的问题,采用自然发酵、酵母菌发酵、混合菌发酵、乳酸菌发酵的样品总酸含量较鲜枣分别上升了20%、18.83%、21.67%、42.50%;总糖含量较鲜枣分别下降了29.18%、34.44%、35.33%、25.17%;发酵结束时还原糖含量分别为3.63%、4.32%、3.20%、3.94%。(2) The new fermented jujube cheese product provided by the present invention has comprehensive nutrition. The total sugar content is lower than that of fresh fruit, but the reducing sugar content is increased, the total acid content is increased, and the total phenolic content is increased. The preparation of the new product solves the problem of existing dried jujube products. Due to the problem of sweet taste caused by excessive sugar content and insufficient acid content, the total acid content of samples using natural fermentation, yeast fermentation, mixed bacteria fermentation, and lactic acid bacteria fermentation increased by 20%, 18.83%, and 21.67% respectively compared with fresh dates. %, 42.50%; the total sugar content decreased by 29.18%, 34.44%, 35.33%, and 25.17% respectively compared with fresh dates; the reducing sugar content at the end of fermentation was 3.63%, 4.32%, 3.20%, and 3.94% respectively.

(3)本发明提供的发酵新产品枣酪干,采用混合菌发酵,当酵母菌-乳酸菌按照质量比1:1接种,发酵过程中的总糖含量最低为25.73%,总酚含量最高为47.83mg/g,。(3) The new fermented date cheese product provided by the present invention is fermented by mixed bacteria. When the yeast-lactic acid bacteria are inoculated according to the mass ratio of 1:1, the total sugar content during the fermentation process is the lowest 25.73%, and the total phenol content is the highest 47.83 mg/g,.

说明书附图Instructions with pictures

图1为不同鲜果预处理方式的枣酪干在发酵过程中总酚含量的变化图。Figure 1 shows the changes in the total phenolic content of dried date cheese during the fermentation process with different fresh fruit pretreatment methods.

图2为不同鲜果预处理方式的枣酪干在发酵过程中总酸含量的变化图。Figure 2 shows the changes in the total acid content of dried date cheese with different fresh fruit pretreatment methods during the fermentation process.

图3为不同鲜果预处理方式的枣酪干在发酵过程中总糖含量的变化图。Figure 3 shows the changes in the total sugar content of dried date cheese during the fermentation process with different fresh fruit pretreatment methods.

图4为不同鲜果预处理方式的枣酪干在发酵过程中还原糖含量的变化图。Figure 4 is a diagram showing the changes in reducing sugar content of dried date cheese with different fresh fruit pretreatment methods during the fermentation process.

图5为不同发酵方式的枣酪干在发酵过程中总酚含量的变化图。Figure 5 shows the changes in the total phenolic content of dried date cheese during the fermentation process using different fermentation methods.

图6为不同发酵方式的枣酪干在发酵过程中总酸含量的变化图。Figure 6 shows the changes in the total acid content of dried date cheese during the fermentation process using different fermentation methods.

图7为不同发酵方式的枣酪干在发酵过程中总糖含量的变化图。Figure 7 is a graph showing changes in the total sugar content of dried date cheese during the fermentation process using different fermentation methods.

图8为不同发酵方式的枣酪干在发酵过程中还原糖含量的变化图。Figure 8 is a diagram showing the changes in reducing sugar content of dried date cheese during the fermentation process using different fermentation methods.

具体实施方式Detailed ways

下面,举实施例说明本发明,但是,本发明并不限于下述的实施例。本发明中选用的所有原辅材料,以及选用的菌种培养方法都为本领域熟知选用的,本发明中涉及到的%都为重量百分比,除非特别指出除外。The present invention will be described below with reference to examples. However, the present invention is not limited to the following examples. All raw and auxiliary materials selected in the present invention, as well as the selected bacterial culture methods are well known in the art. The % mentioned in the present invention are all weight percentages, unless otherwise specified.

实施例1:枣酪干的制备Example 1: Preparation of dried date cheese

本实施例提供一种发酵新产品枣酪干,该枣酪干采用如下步骤制备:新鲜枣果清洗预处理后,加入浓度为0.5%的柠檬酸护色处理10min;将处理后的枣果装入15×25cm真空袋,按照占枣果重量4%接种悬菌液封口,于37±1℃发酵60-84小时;将发酵后的枣果于55-60℃烘制10-12小时至水分含量为20%,真空包装常压水浴于80℃杀菌20min即得新产品枣酪干。This embodiment provides a new fermented product, dried date cheese, which is prepared by the following steps: after washing and pre-processing the fresh date fruit, add citric acid with a concentration of 0.5% for color protection treatment for 10 minutes; pack the processed date fruit into Put it into a 15×25cm vacuum bag, inoculate 4% of the weight of the jujube fruit with a suspension, seal it, and ferment it at 37±1℃ for 60-84 hours; bake the fermented jujube fruit at 55-60℃ for 10-12 hours until the moisture content The content is 20%, vacuum-packed and sterilized in a normal pressure water bath at 80°C for 20 minutes to obtain a new product of dried date cheese.

其中,所述枣果为骏枣、灰枣、哈密大枣中的任意一种,所述鲜枣预处理方式为去皮去核不刺孔、不去皮去核刺孔和不去皮去核不刺孔中的任意一种;发酵方式为自然发酵、酵母菌发酵、混合菌发酵、乳酸菌发酵中的任意一种。Wherein, the jujube fruit is any one of jujube, gray jujube, and Hami jujube, and the pretreatment method of the fresh jujube is peeling and pitting without puncturing, peeling and pitting without puncturing, and peeling without peeling. The core does not puncture any one; the fermentation method is any one of natural fermentation, yeast fermentation, mixed bacteria fermentation, and lactic acid bacteria fermentation.

实施例2:枣酪干的制备Example 2: Preparation of dried date cheese

本实施例在实施例1的基础上,提供一种发酵新产品枣酪干的制备方法,其中,枣果为骏枣,鲜枣预处理方式为不去皮去核不刺孔;发酵方式为混合菌发酵;混合菌菌悬液按照如下步骤获得:酵母菌:乳酸菌按照质量比1:1溶于30±0.5℃温水中,制成浓度为20%的悬菌液,活化30min。On the basis of Example 1, this embodiment provides a method for preparing a new fermented jujube cheese product, in which the jujube fruit is jujube, and the fresh jujube pretreatment method is no peeling, core removal, and no punctures; the fermentation method is: Mixed bacterial fermentation; mixed bacterial suspension is obtained according to the following steps: yeast: lactic acid bacteria are dissolved in warm water at 30±0.5°C according to a mass ratio of 1:1 to prepare a suspension with a concentration of 20%, and activated for 30 minutes.

实施例3:枣酪干的制备Example 3: Preparation of dried date cheese

本实施例在实施例1的基础上,提供一种发酵新产品枣酪干的制备方法,其中,枣果为哈密大枣,鲜枣预处理方式为去皮去核不刺孔;发酵方式为37±1℃温度条件下自然发酵。On the basis of Example 1, this embodiment provides a method for preparing a new fermented jujube cheese product, in which the jujube fruit is Hami jujube, and the fresh jujube pretreatment method is to peel, remove and core without puncturing; the fermentation method is: Natural fermentation at a temperature of 37±1°C.

实施例4:枣酪干的制备Example 4: Preparation of dried date cheese

本实施例在实施例1的基础上,提供一种发酵新产品枣酪干的制备方法,其中,枣果为灰枣,鲜枣预处理方式为不去皮去核刺孔;发酵方式为酵母菌发酵,酵母菌悬菌液采用如下步骤制备:取酵母菌粉60g溶于300mL 30±0.5℃温水中,制成浓度为20%的悬菌液,活化30min获得。On the basis of Example 1, this embodiment provides a method for preparing a new fermented jujube cheese product, in which the jujube fruit is gray jujube, the fresh jujube pretreatment method is non-peeling, pitting and punctures; the fermentation method is yeast For yeast fermentation, the yeast suspension is prepared using the following steps: Dissolve 60g of yeast powder in 300mL of 30±0.5°C warm water to make a suspension with a concentration of 20%, and activate it for 30 minutes to obtain it.

实施例5:枣酪干的制备Example 5: Preparation of dried date cheese

本实施例在实施例1的基础上,提供一种发酵新产品枣酪干的制备方法,其中,枣果为骏枣,鲜枣预处理方式为不去皮去核不刺孔;发酵方式为乳酸菌发酵,乳酸菌悬菌液采用如下步骤制备:取乳酸菌粉60g溶于300mL30±0.5℃温水中,制成浓度为20%的悬菌液,活化30min获得。On the basis of Example 1, this embodiment provides a method for preparing a new fermented jujube cheese product, in which the jujube fruit is jujube, and the fresh jujube pretreatment method is no peeling, core removal, and no punctures; the fermentation method is: For lactic acid bacteria fermentation, the lactic acid bacteria suspension is prepared using the following steps: Dissolve 60g of lactic acid bacteria powder in 300 mL of 30±0.5°C warm water to prepare a suspension with a concentration of 20%, and activate it for 30 minutes.

实施例6:枣酪干的制备Example 6: Preparation of dried date cheese

本实施例在实施例1的基础上,提供一种发酵新产品枣酪干的制备方法,其中,枣果为骏枣,鲜枣预处理方式为不去皮去核刺孔;发酵方式为混合菌发酵,混合菌悬菌液采用如下步骤制备:取乳酸菌粉、酵母菌粉各30g分别溶于300mL30±0.5℃温水中,制成浓度为20%的悬菌液,活化30min获得。On the basis of Example 1, this embodiment provides a method for preparing a new fermented jujube cheese product, in which the jujube fruit is jujube, the fresh jujube pretreatment method is not peeling, pitting and puncturing; the fermentation method is mixing Bacteria fermentation, the mixed bacterial suspension is prepared using the following steps: Dissolve 30g each of lactic acid bacteria powder and yeast powder in 300mL of 30±0.5°C warm water to prepare a suspension with a concentration of 20%, and activate it for 30 minutes.

实施例7:枣酪干制备工艺的优化Example 7: Optimization of the preparation process of dried date cheese

本实施例在实施例1-6的基础上,对发酵新产品枣酪干的制备工艺进行优化,考察预处理方式、发酵方式对产品发酵过程中总酚、总酸、总糖和还原糖含量的影响。In this example, on the basis of Examples 1-6, the preparation process of the new fermented date cheese dried product is optimized, and the effect of pretreatment method and fermentation method on the total phenol, total acid, total sugar and reducing sugar content during the fermentation process of the product is investigated. Impact.

1.鲜枣预处理方式对产品性能的影响1. Effect of fresh jujube pretreatment methods on product performance

(1)试验方案(1)Test plan

原材料:新鲜骏枣:2020年9月采自阿克苏,全红脆熟、色泽均一、无病虫害、无机械损伤、运至冷库备用。双歧杆菌乳酸菌粉:川秀有限公司;酵母菌粉:安琪公司;福林酚,索莱宝公司;L(+)-抗坏血酸标准品(纯度≥99%)国药集团化学试剂有限公司,葡萄糖、浓硫酸、乙醇、硫酸铜,酒石酸钾钠、氢氧化钠、亚铁氰化钾、乙酸锌、甲基红、酚酞、盐酸、冰乙酸、邻苯二甲酸氢钾(均为分析纯)。Raw materials: Fresh jujubes: collected from Aksu in September 2020, all red, crisp and ripe, with uniform color, no pests and diseases, no mechanical damage, transported to cold storage for later use. Bifidobacterium lactobacillus powder: Chuanxiu Co., Ltd.; Yeast powder: Angel Company; Fulinol, Solebao Company; L(+)-ascorbic acid standard (purity ≥99%) Sinopharm Chemical Reagent Co., Ltd., glucose , concentrated sulfuric acid, ethanol, copper sulfate, potassium sodium tartrate, sodium hydroxide, potassium ferrocyanide, zinc acetate, methyl red, phenolphthalein, hydrochloric acid, glacial acetic acid, potassium hydrogen phthalate (all analytical grade).

鲜枣预处理方式:新鲜骏枣清洗后,分别进行去皮去核不刺孔(处理一)、不去皮去核刺孔(处理二)、不去皮去核不刺孔(处理三)三种预处理。碱液去皮:设置浓度分别为:2%、3%、4%、5%,根据去皮效果选择3%的碱液浓度。将不同预处理的枣果装入15×25cm真空袋内,按枣果重注入4%乳酸菌悬液液(经活化含20%乳酸菌),采用真空抽渗并封口,于37±1℃下发酵84h,得到成品。期间每12h取一次样,分析制品的还原糖、总酚、总酸、总糖等指标,选择出最适合的预处理。Fresh jujube pretreatment method: After cleaning the fresh jujube, peel and core without puncture (process one), peel without peel and core without puncture (process two), and peel without peel and core without puncture (process three) Three kinds of preprocessing. Alkali solution peeling: Set the concentrations as: 2%, 3%, 4%, 5% respectively. Select the lye solution concentration of 3% according to the peeling effect. Put the jujube fruits with different pretreatments into a 15×25cm vacuum bag, inject 4% lactic acid bacteria suspension (containing 20% lactic acid bacteria after activation) according to the weight of the jujube fruits, use vacuum extraction and sealing, and ferment at 37±1℃ 84h, got the finished product. During this period, samples were taken every 12 hours, and the reducing sugar, total phenols, total acid, total sugar and other indicators of the product were analyzed to select the most suitable pretreatment.

(2)试验结果分析(2) Analysis of test results

表1:鲜枣部分品质指标Table 1: Some quality indicators of fresh dates

表1为本试验对鲜枣的总酸、总酚、总糖及还原糖含量进行测定,作为对照,考察不同预处理方式发酵后产品营养成分的变化,具体结果参见附图1-4所示。Table 1 shows the determination of the total acid, total phenols, total sugar and reducing sugar content of fresh dates in this experiment. As a control, the changes in the nutritional content of the product after fermentation with different pretreatment methods are examined. The specific results are shown in Figures 1-4. .

附图1为不同鲜果预处理方式的枣酪干在发酵过程中总酚含量的变化图。由附图1数据可知,三种不同预处理方式进行发酵,产品的发酵过程中总酚含量变化总体呈先上升再下降的趋势。其中,采用预处理方式处理一时,枣酪干在发酵过程中总酚含量呈现先升高后降低的趋势,总差值达到35.48mg/g,总酚含量于48h达到最大值58.97mg/g,60-84h期间总酚含量的变化趋于平稳;采用预处理方式处理二时,枣酪干的总酚含量在48h达到最大值64.30mg/g,相较于处理一其总酚含量提升速率较快,于12h达到次高峰;采用预处理方式处理三时,枣酪干的总酚含量显著高于其它两个预处理方式,总酚同样在48h达到峰值,为112.20mg/g,上升速率较处理二更快。表明鲜枣经乳酸发酵在一定时间内总酚含量提高,抗氧化能力增强,三个预处理中处理三明显高于其余两组(p<0.05)。Figure 1 is a graph showing changes in the total phenolic content of dried date cheese during the fermentation process with different fresh fruit pretreatment methods. It can be seen from the data in Figure 1 that when three different pretreatment methods were used for fermentation, the changes in the total phenolic content of the product during the fermentation process showed an overall trend of first increasing and then decreasing. Among them, after pretreatment, the total phenolic content of dried date cheese showed a trend of first increasing and then decreasing during the fermentation process. The total difference reached 35.48mg/g, and the total phenolic content reached the maximum value of 58.97mg/g at 48h. The changes in total phenolic content tended to be stable during 60-84h; when pretreatment was used in the second treatment, the total phenolic content of dried date cheese reached the maximum value of 64.30mg/g at 48h. Compared with treatment one, the total phenolic content increased at a slower rate. Fast, reaching the second peak at 12h; when using the pretreatment method for three hours, the total phenolic content of dried date cheese was significantly higher than the other two pretreatment methods. The total phenol also reached the peak at 48h, which was 112.20 mg/g, and the rising rate was faster than that of the other two pretreatment methods. Processing two is faster. It showed that the total phenolic content of fresh dates increased after lactic acid fermentation within a certain period of time, and the antioxidant capacity was enhanced. Among the three pretreatments, treatment three was significantly higher than the other two groups (p<0.05).

附图2为不同鲜果预处理方式的枣酪干在发酵过程中总酸含量的变化图。总酸是表示果实品质变化的重要指标,也是评价发酵速率及制品品质重要指标。由图2数据可知,不同的预处理方式发酵的产品在整个发酵过程中总酸含量总体呈先升高后降低的趋势。其中,处理一、处理二、处理三总酸达到高峰值的发酵时间分别为36h、12h、48h,说明鲜枣预处理方式中,处理二更有利于提升枣果的乳酸发酵速度,发酵结束时,各处理总酸含量均低于鲜枣(0.6%),这可能是由于接入乳酸菌进行乳酸发酵的同时,枣果自然携带的异型乳酸菌也进行异形乳酸发酵,产物除乳酸外,还产生乙醇、乙酸和二氧化碳等多种产物,之后发生酯化反应,消耗一部分酸。三种预处理方式发酵产品总酸的含量至发酵84h结束时分别下降了27.74%、42.97%、45.84%,此时总酸含量分别为0.41%、0.30%、0.46%;处理三较处理一、处理二具有显著差异(p<0.05)。Figure 2 is a graph showing changes in the total acid content of dried date cheese during the fermentation process with different fresh fruit pretreatment methods. Total acid is an important indicator of changes in fruit quality, as well as an important indicator for evaluating fermentation rate and product quality. It can be seen from the data in Figure 2 that the total acid content of products fermented by different pretreatment methods showed an overall trend of first increasing and then decreasing during the entire fermentation process. Among them, the fermentation times for total acid to reach the peak in treatments one, two and three were 36h, 12h and 48h respectively, indicating that among the fresh jujube pretreatment methods, treatment two is more conducive to increasing the lactic acid fermentation speed of jujube fruits. At the end of fermentation , the total acid content in each treatment was lower than that of fresh dates (0.6%). This may be due to the fact that when lactic acid bacteria are introduced for lactic acid fermentation, the heterolactic acid bacteria naturally carried by the jujube fruit also undergo heteroformal lactic acid fermentation. In addition to lactic acid, ethanol is also produced. , acetic acid and carbon dioxide and other products, and then an esterification reaction occurs, consuming part of the acid. The total acid content of the fermentation products of the three pretreatment methods decreased by 27.74%, 42.97%, and 45.84% respectively by the end of 84 hours of fermentation. At this time, the total acid content was 0.41%, 0.30%, and 0.46% respectively; the three treatments were compared with treatment one and Treatment 2 has significant differences (p<0.05).

附图3为不同鲜果预处理方式的枣酪干在发酵过程中总糖含量的变化图,由图3数据可知,在发酵12h时,各组总糖的含量变化处理一、处理二、处理三总糖含量与初始值相比差异均显著(P<0.05),此时样品与鲜枣相比分别下降了20.19%、11.05%、7.12%;发酵48h前,处理一总糖含量处于最低水平,说明去皮后发酵速度最快;发酵48h至72h期间,各组总糖含量降低速率差异不明显,分别下降了17.23%、24.46%、29.05%;发酵84h时,处理二总糖上升了5.45%,这可能是由于微生物的生长繁殖和代谢导致发酵液中的总糖浓度下降与含糖代谢产物的产生共同影响造成的。综上表明,发酵期间样品总糖含量整体呈下降的趋势;发酵结束时,在三个处理中处理二明显高于其余两组(P<0.05)。Figure 3 is a graph showing changes in the total sugar content of dried date cheese during the fermentation process with different fresh fruit pretreatment methods. From the data in Figure 3, it can be seen that at 12 hours of fermentation, the changes in total sugar content in each group were treated with treatment one, treatment two, and treatment three. The total sugar content was significantly different from the initial value (P<0.05). At this time, the sample decreased by 20.19%, 11.05%, and 7.12% respectively compared with fresh dates. Before fermentation for 48 hours, the total sugar content in treatment 1 was at the lowest level. It shows that the fermentation speed is the fastest after peeling; from 48h to 72h of fermentation, the reduction rate of total sugar content in each group was not obvious, which decreased by 17.23%, 24.46%, and 29.05% respectively; at 84h of fermentation, the total sugar content of the treatment increased by 5.45% , this may be due to the combined impact of the decrease in the total sugar concentration in the fermentation broth and the production of sugar-containing metabolites due to the growth, reproduction and metabolism of microorganisms. In summary, the total sugar content of the samples showed an overall downward trend during the fermentation period; at the end of the fermentation, among the three treatments, treatment two was significantly higher than the other two groups (P<0.05).

附图4为不同鲜果预处理方式的枣酪干在发酵过程中还原糖含量的变化图,由图4数据可知,三种不同预处理中,除处理一在36h外,其余产品还原糖含量在发酵过程12h-72h中均处于上升趋势,处理二、处理三上升速率分别为83.19%、13.75%;同时,三种处理间差异均显著(p<0.05)。发酵时间为12h时,三种不同预处理方式样品中还原糖上升速率无显著差异,与鲜枣相比上升速率分别为32.77%、33.42%、14.93%;发酵84h时,处理一、处理二、处理三的还原糖呈现下降趋势,下降速率分别为8.07%、19.45%、4.20%,此时处理三还原糖含量最高,为4.13g/100g。综合考虑,处理三发酵结束时,总酸为0.46%,总糖为25.73%,糖酸比为55.93,制品风味最佳,果型保持最好,选择处理三(不去皮去核不刺孔)为较优预处理。Figure 4 is a graph showing the changes in the reducing sugar content of dried dates cheese with different fresh fruit pretreatment methods during the fermentation process. From the data in Figure 4, it can be seen that among the three different pretreatments, except for treatment one at 36h, the reducing sugar content of the other products is at The fermentation process was on an upward trend from 12h to 72h, and the rising rates of treatments two and three were 83.19% and 13.75% respectively; at the same time, the differences among the three treatments were significant (p<0.05). When the fermentation time is 12 hours, there is no significant difference in the rising rate of reducing sugar in the samples with three different pretreatment methods. Compared with fresh dates, the rising rates are 32.77%, 33.42%, and 14.93% respectively; when the fermentation time is 84 hours, the rising rates of reducing sugars in the samples of three different pretreatment methods are 32.77%, 33.42%, and 14.93% respectively. The reducing sugars in treatment three showed a downward trend, and the decreasing rates were 8.07%, 19.45%, and 4.20% respectively. At this time, the reducing sugar content in treatment three was the highest, which was 4.13g/100g. Comprehensive consideration, at the end of fermentation in treatment three, the total acid was 0.46%, the total sugar was 25.73%, and the sugar-acid ratio was 55.93. The product had the best flavor and the fruit shape was best maintained. Therefore, treatment three (no peeling, corering, and no punctures) was selected. ) is the better preprocessing.

2.不同发酵方式对产品性能的影响2. The impact of different fermentation methods on product performance

(1)试验方案(1)Test plan

原材料:新鲜骏枣:2020年9月采自阿克苏,全红脆熟、色泽均一、无病虫害、无机械损伤、运至冷库备用。双歧杆菌乳酸菌粉:川秀有限公司;酵母菌粉:安琪公司;福林酚,索莱宝公司;L(+)-抗坏血酸标准品(纯度≥99%)国药集团化学试剂有限公司,葡萄糖、浓硫酸、乙醇、硫酸铜,酒石酸钾钠、氢氧化钠、亚铁氰化钾、乙酸锌、甲基红、酚酞、盐酸、冰乙酸、邻苯二甲酸氢钾(均为分析纯)。Raw materials: Fresh jujubes: collected from Aksu in September 2020, all red, crisp and ripe, with uniform color, no pests and diseases, no mechanical damage, transported to cold storage for later use. Bifidobacterium lactobacillus powder: Chuanxiu Co., Ltd.; Yeast powder: Angel Company; Fulinol, Solebao Company; L(+)-ascorbic acid standard (purity ≥99%) Sinopharm Chemical Reagent Co., Ltd., glucose , concentrated sulfuric acid, ethanol, copper sulfate, potassium sodium tartrate, sodium hydroxide, potassium ferrocyanide, zinc acetate, methyl red, phenolphthalein, hydrochloric acid, glacial acetic acid, potassium hydrogen phthalate (all analytical grade).

鲜枣预处理方式:不去皮去核不刺孔。Pre-processing method for fresh dates: no peeling, no pitting, no punching.

发酵方式:Fermentation method:

自然发酵:取新鲜骏枣2kg,经清洗,选择最优预处理,经抽真空封入15×25cm袋内,37±1℃温度条件下发酵,发酵过程中定期取样进行总酚,总酸,总糖及还原糖的含量的测定,在前期预试验的基础上确定发酵总时间为84h。取样时间点为:12h、24h、36h、48h、60h、72h及84h。Natural fermentation: Take 2kg of fresh jujubes, wash them, select the optimal pre-treatment, seal them in a 15×25cm bag under vacuum, and ferment at a temperature of 37±1°C. During the fermentation process, samples are taken regularly for total phenols, total acids, and total For the determination of sugar and reducing sugar content, the total fermentation time was determined to be 84 hours based on the preliminary test. The sampling time points are: 12h, 24h, 36h, 48h, 60h, 72h and 84h.

乳酸菌发酵:取乳酸菌粉60g溶于300mL30±0.5℃温水中,制成20%的悬菌液,活化30min,待用。取新鲜骏枣2kg,经预处理后装入真空袋内,注入枣果重4%的悬菌液,抽真空并封口;于37±1℃温度条件下发酵,发酵过程中定期取样进行总酚,总酸,总糖及还原糖含量的测定,在前期预试验基础上确定发酵总时间为84h。取样时间点为:12h、24h、36h、48h、60h、72h及84h。Lactic acid bacteria fermentation: Dissolve 60g of lactic acid bacteria powder in 300mL of 30±0.5°C warm water to make a 20% suspension, activate it for 30 minutes, and set aside. Take 2kg of fresh jujube, put it into a vacuum bag after pretreatment, inject 4% of the weight of jujube fruit suspension, vacuum and seal; ferment at a temperature of 37±1°C. During the fermentation process, samples are taken regularly for total phenolics. , the determination of total acid, total sugar and reducing sugar content, the total fermentation time was determined to be 84h based on the preliminary test. The sampling time points are: 12h, 24h, 36h, 48h, 60h, 72h and 84h.

酵母菌发酵:取酵母菌粉60g溶于300mL30±0.5℃温水中,制成20%的悬菌液,活化30min,待用。取新鲜骏枣2kg,经预处理后装入真空袋内,注入枣果重4%的悬菌液,抽真空并封口;于37±1℃温度条件下发酵,发酵过程中定期取样进行总酚,总酸,总糖及还原糖的含量的测定,在前期预试验的基础上确定发酵总时间为84h。取样时间点为:12h、24h、36h、48h、60h、72h及84h。Yeast fermentation: Dissolve 60g of yeast powder in 300mL of 30±0.5°C warm water to make a 20% suspension, activate for 30 minutes, and set aside. Take 2kg of fresh jujube, put it into a vacuum bag after pretreatment, inject 4% of the weight of jujube fruit suspension, vacuum and seal; ferment at a temperature of 37±1°C. During the fermentation process, samples are taken regularly for total phenolics. , the content of total acid, total sugar and reducing sugar was determined, and the total fermentation time was determined to be 84h based on the preliminary test. The sampling time points are: 12h, 24h, 36h, 48h, 60h, 72h and 84h.

混合菌发酵:取乳酸菌粉、酵母菌粉各30g分别溶于300mL30±0.5℃温水中,制成20%的悬菌液,活化30min,待用。取新鲜骏枣2kg,经预处理后装入真空袋内,分别注入枣果重2%(共4%)的悬菌液,抽真空并封口;于37±1℃温度条件下发酵,发酵过程中定期取样进行总酚,总酸,总糖及还原糖的含量的测定,在前期预试验的基础上确定发酵总时间为84h。取样时间点为:12h、24h、36h、48h、60h、72h及84h。Mixed bacteria fermentation: Dissolve 30g each of lactic acid bacteria powder and yeast powder in 300mL 30±0.5℃ warm water to make a 20% suspension, activate it for 30 minutes, and set aside. Take 2kg of fresh jujubes, put them into vacuum bags after pretreatment, inject 2% of jujube fruit weight (4% in total) suspension bacteria respectively, vacuum and seal; ferment at 37±1°C, fermentation process Samples were taken regularly to measure the contents of total phenols, total acids, total sugars and reducing sugars. Based on the preliminary test, the total fermentation time was determined to be 84 hours. The sampling time points are: 12h, 24h, 36h, 48h, 60h, 72h and 84h.

(2)试验结果与分析(2)Test results and analysis

酚类物质是一类存在于水果、蔬菜等植物体内的多羟基化合物的总称,也是体现果蔬抗氧化能力的主要物质之一,新鲜骏枣的总酚含量是49.24mg/g,附图5为不同发酵方式的枣酪干在发酵过程中总酚含量的变化图,由图5数据可知,4种不同发酵方式发酵,枣酪干总酚含量于发酵24h和48h时各出现2个高峰值,发酵48h后,产品的总酚含量均呈下降趋势,其中混合菌发酵枣酪干的总酚含量均高于其它3种发酵方式(P<0.05),发酵84h时,自然发酵、酵母菌发酵、混合菌发酵、乳酸菌发酵4种发酵方式所得枣酪干的总酚含量分别为39.02mg/g、38.44mg/g、47.83mg/g、35.78mg/g,较发酵48h时分别下降了29.06%、49.61%、35.70%、44.35%,具有显著性差异(p<0.05),混合菌发酵对枣酪干总酚含量的下降速率有明显的减缓作用。Phenolics are a general term for a class of polyhydroxy compounds that exist in fruits, vegetables and other plants. They are also one of the main substances that reflect the antioxidant capacity of fruits and vegetables. The total phenolic content of fresh jujubes is 49.24mg/g. Figure 5 shows The change diagram of the total phenolic content of dried date cheese in different fermentation methods during the fermentation process. From the data in Figure 5, it can be seen that the total phenolic content of dried date cheese in four different fermentation methods showed two high peaks at 24h and 48h of fermentation. After fermentation for 48 hours, the total phenolic content of the products showed a downward trend. Among them, the total phenolic content of dried date cheese fermented by mixed bacteria was higher than that of the other three fermentation methods (P<0.05). When fermented for 84 hours, natural fermentation, yeast fermentation, The total phenolic contents of dried date cheese obtained from the four fermentation methods of mixed bacteria fermentation and lactic acid bacteria fermentation were 39.02 mg/g, 38.44 mg/g, 47.83 mg/g, and 35.78 mg/g respectively, which were 29.06%, 29.06% lower than that at 48 h of fermentation, respectively. 49.61%, 35.70%, 44.35%, with significant differences (p<0.05). Mixed bacterial fermentation has a significant slowing down rate of decline in the total phenolic content of dried date cheese.

附图6为不同发酵方式的枣酪干在发酵过程中总酸含量的变化图,由图6数据可知,除自然发酵样品总酸含量随着发酵进程呈现先上升后下降趋势外,其它3种发酵方式样品总酸含量均呈下降、上升、再下降趋势,4种发酵方式样品总酸含量于48h时均达到最高值,此时自然发酵、酵母菌发酵、混合菌发酵、乳酸菌发酵方式总酸含量为0.48%、0.71%、0.73%、0.85%,较鲜枣分别上升了20%、18.83%、21.67%、42.50%;48h后,4种发酵方式的总酸含量均快速下降,至84h发酵结束时自然发酵、酵母菌发酵、混合菌发酵、乳酸菌发酵样品的总酸含量分别为0.29%、0.38%、0.45%、0.46%。分析发酵不同方式与发酵过程中总酸的变化趋势可以看出,自然发酵方式发酵主要由原料自身携带的菌发酵,乳酸发酵速度较慢同时产酸量也较低,接种乳酸菌、酵母菌及混合菌发酵的样品在原料自身携带菌乳酸发酵产酸的同时叠加了优势乳酸菌发酵产生的乳酸,而接种酵母菌的样品也会在酒精发酵的同时产生二氧化碳增加部分酸度,所以在发酵过程及发酵结束时4种发酵方式样品的总酸含量的顺序是自然发酵<酵母菌发酵<混合菌发酵<乳酸菌发酵,48h后发酵过程中,总酸含量均降低的原因可能是后期酯类物质的生成消耗部分有机酸所致。Figure 6 is a graph showing changes in the total acid content of dried date cheese produced in different fermentation methods during the fermentation process. From the data in Figure 6, it can be seen that, except for the total acid content of the natural fermentation sample, which first increased and then decreased with the fermentation process, the other three types of The total acid content of the fermentation method samples showed a trend of decreasing, increasing, and then decreasing. The total acid content of the four fermentation method samples reached the highest value at 48 hours. At this time, the total acid content of natural fermentation, yeast fermentation, mixed bacteria fermentation, and lactic acid bacteria fermentation method The contents were 0.48%, 0.71%, 0.73%, and 0.85%, which increased by 20%, 18.83%, 21.67%, and 42.50% respectively compared with fresh dates; after 48 hours, the total acid content of the four fermentation methods dropped rapidly, and reached 84 hours of fermentation. At the end, the total acid content of natural fermentation, yeast fermentation, mixed bacteria fermentation, and lactic acid bacteria fermentation samples were 0.29%, 0.38%, 0.45%, and 0.46% respectively. Analyzing different fermentation methods and the changing trend of total acid during the fermentation process, it can be seen that natural fermentation fermentation is mainly fermented by bacteria carried by the raw materials themselves. The lactic acid fermentation speed is slow and the acid production is also low. Inoculation of lactic acid bacteria, yeast and mixed Samples fermented by bacteria carry the lactic acid fermentation of the raw materials and are superimposed with the lactic acid produced by the fermentation of the dominant lactic acid bacteria. Samples inoculated with yeast will also produce carbon dioxide during alcoholic fermentation to increase some acidity, so during the fermentation process and the end of fermentation, The order of the total acid content of samples from the four fermentation methods is natural fermentation < yeast fermentation < mixed bacteria fermentation < lactic acid bacteria fermentation. The reason why the total acid content decreased during the fermentation process after 48 hours may be due to the production and consumption of esters in the later stage. Caused by organic acids.

附图7为不同发酵方式的枣酪干在发酵过程中总糖含量的变化图,由图7数据可知,4种发酵方式枣酪干发酵过程中总糖含量均逐渐下降,自然发酵样品除12h外其它各时间段残糖量均处于最高值,同时也说明自然发酵速度慢、残糖高,48h时总糖含量与另外3组差异极显著(P<0.05),下降幅度为12.28%。发酵结束时,自然发酵、酵母菌发酵、混合菌发酵、乳酸菌发酵的样品总糖含量分别为24.22%、20.63%、19.69%、23.21%,较鲜枣分别下降了29.18%、34.44%、35.33%、25.17%,混合菌发酵的样品残糖量最低。这主要是因为乳酸、酒精的发酵作用产生有机酸的同时消耗了原料中部分糖。Figure 7 is a graph showing changes in the total sugar content of dried dates and cheese during the fermentation process of different fermentation methods. From the data in Figure 7, it can be seen that the total sugar content of dried dates and cheese in the four fermentation methods gradually decreased during the fermentation process. The natural fermentation samples except for 12h In addition, the residual sugar content in other time periods was at the highest value, which also showed that the natural fermentation speed was slow and the residual sugar was high. The total sugar content at 48 hours was significantly different from the other three groups (P<0.05), with a decrease of 12.28%. At the end of fermentation, the total sugar content of samples from natural fermentation, yeast fermentation, mixed bacteria fermentation, and lactic acid bacteria fermentation was 24.22%, 20.63%, 19.69%, and 23.21% respectively, which were 29.18%, 34.44%, and 35.33% lower than that of fresh dates, respectively. , 25.17%, and the residual sugar content of the sample fermented by mixed bacteria was the lowest. This is mainly because the fermentation of lactic acid and alcohol produces organic acids and consumes part of the sugar in the raw materials.

附图8为不同发酵方式的枣酪干在发酵过程中还原糖含量的变化图,由图8数据可知,4种发酵方式发酵的枣酪干在发酵过程中还原糖含量均呈上升趋势,其中,发酵48h前上升趋势较明显,发酵结束时4种发酵方式发酵样品还原糖含量均高于12h时的样品;发酵48h后至发酵结束时酵母菌发酵样品还原糖含量最高,为4.32%,混合菌发酵样品还原糖含量最低,为3.20%。自然发酵、混合菌液发酵、酵母菌液发酵至48h时上升速率分别为43.8%、56.3%、54.8%;发酵结束时自然发酵、酵母菌发酵、混合菌发酵、乳酸菌发酵4种发方式所得样品还原糖含量分别为3.63%、4.32%、3.20%、3.94%。经混合菌发酵的样品与其它3种发酵方式呈显著差异(p<0.05)。Figure 8 is a graph showing changes in the reducing sugar content of dried dates fermented by different fermentation methods during the fermentation process. From the data in Figure 8, it can be seen that the reducing sugar content of dried dates fermented by the four fermentation methods showed an increasing trend during the fermentation process. Among them, , the rising trend is obvious before 48 hours of fermentation. At the end of fermentation, the reducing sugar content of the fermentation samples of the four fermentation methods is higher than that of the samples at 12 hours; after 48 hours of fermentation to the end of fermentation, the reducing sugar content of the yeast fermentation sample is the highest, which is 4.32%. Mixed The reducing sugar content of bacterial fermentation samples was the lowest, 3.20%. The rising rates of natural fermentation, mixed bacterial liquid fermentation, and yeast liquid fermentation to 48 hours were 43.8%, 56.3%, and 54.8% respectively; at the end of fermentation, samples were obtained from the four fermentation methods of natural fermentation, yeast fermentation, mixed bacterial fermentation, and lactic acid bacteria fermentation. The reducing sugar contents are 3.63%, 4.32%, 3.20%, and 3.94% respectively. The samples fermented by mixed bacteria were significantly different from the other three fermentation methods (p<0.05).

综上实验数据分析可知,鲜骏枣采用去皮去核不刺孔、不去皮去核刺孔、不去皮去核不刺孔三种不同的预处理方式,经乳酸菌发酵,通过综合分析发酵过程中总酸、总酚、总糖及还原糖的变化及风味,筛选出鲜枣发酵前采用不去皮去核不刺孔的预处理方式为较优处理方式,总糖含量较低,总酚含量较高;将鲜骏枣用优选的预处理方式处理后,分别采用自然发酵、乳酸菌发酵、酵母菌发酵、乳酸-酵母菌混合发酵四种不同菌剂的发酵方式进行发酵,以发酵过程中还原糖、总酸、总糖、总酚含量的变化趋势为评价指标,得出发酵枣酪的最佳发酵方式为酵母菌-乳酸菌混合发酵的发酵方式,接种比例1:1,总糖含量最低为25.73%,总酚含量最高为47.83mg/g。In summary, the above experimental data analysis shows that fresh jujube adopts three different pretreatment methods: peeled, cored and not punctured, not peeled, cored and punctured, and not peeled, cored and not punctured. After fermentation by lactic acid bacteria, through comprehensive analysis During the fermentation process, the changes and flavor of total acid, total phenols, total sugar and reducing sugar were screened out, and the pretreatment method of fresh dates without peeling, pitting or puncturing before fermentation was selected as the better treatment method, with lower total sugar content. The total phenolic content is high; after the fresh jujubes are treated with the preferred pretreatment method, they are fermented using four different fermentation methods: natural fermentation, lactic acid bacteria fermentation, yeast fermentation, and lactic acid-yeast mixed fermentation. The changing trends of reducing sugar, total acid, total sugar, and total phenolic contents during the process were used as evaluation indicators. It was concluded that the best fermentation method for fermented date cheese is yeast-lactic acid bacteria mixed fermentation. The inoculation ratio is 1:1, and the total sugar content is 1:1. The lowest content is 25.73%, and the highest total phenolic content is 47.83mg/g.

实施例8:枣酪干与常见枣制品对比Example 8: Comparison between dried date cheese and common date products

为了进一步考察本发明发酵新产品的性能,本试验对本发明实施例2获得的发酵新产品枣酪干与枣制品膨化枣片、膨化半枣进行了品质指标对比,具体结果参见表1所示。In order to further examine the performance of the new fermented product of the present invention, this test compared the quality indicators of the new fermented product dried jujube obtained in Example 2 of the present invention with the jujube products puffed jujube slices and puffed half jujubes. The specific results are shown in Table 1.

表1:枣酪干与其它枣加工产品部分品质指标对比Table 1: Comparison of some quality indicators between dried date cheese and other processed date products

由表1数据分析可知,经鲜枣经乳酸菌酵母菌混合发酵后所得制品与采用膨化干燥所得制品相比,每克干物质中总糖、还原糖含量均大大降低、糖酸比也降低,从口感评价制品的甜度降低酸味提升,明显改善了制品风味。表明本发明提供的发酵新产品不仅在口感方面有显著改善,而且品质优良,为一种全新的休闲食品且更符合健康理念。It can be seen from the data analysis in Table 1 that the total sugar and reducing sugar content per gram of dry matter are greatly reduced, and the sugar-acid ratio is also reduced, compared with the products obtained by puffing and drying the products obtained by mixed fermentation of fresh dates with lactic acid bacteria and yeasts. Taste evaluation: The sweetness of the product is reduced and the sourness is increased, which significantly improves the flavor of the product. It shows that the new fermented product provided by the present invention not only has a significant improvement in taste, but also has excellent quality. It is a brand-new snack food and is more in line with the health concept.

上述实施例仅仅是为清楚地说明本发明所作的举例,而并非对实施方式的限定。对于所属领域的普通技术人员来说,在上述说明的基础上还可以做出其它不同形式的变化或变动。这里无需也无法对所有的实施方式予以穷举。而由此所延伸出的显而易见的变化或变动仍处于本发明的保护范围之中。The above-described embodiments are merely examples to clearly illustrate the present invention, and are not intended to limit the implementation. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. An exhaustive list of all implementations is neither necessary nor possible. However, obvious changes or changes derived therefrom are still within the protection scope of the present invention.

Claims (2)

1.一种枣酪干,其特征在于,该枣酪干通过新鲜枣果接种混合菌发酵烘干获得;具体采用如下步骤:新鲜枣果清洗后采用不去皮去核不刺孔预处理后,加入浓度为0.5%的柠檬酸护色处理10min;将处理后的枣果装入15×25cm真空袋,按照占枣果重量4%接种混合菌悬菌液抽真空封口,于37±1℃发酵60-84小时;将发酵后的枣果于55-60℃烘制10-12小时至水分含量为20%,真空包装常压水浴于80℃杀菌20min即得新产品枣酪干;所述混合菌悬菌液按照如下步骤获得:酵母菌:双歧杆菌按照质量比1:1溶于30±0.5℃温水中,制成浓度为20%的悬菌液,活化30min。1. A kind of dried jujube cheese, characterized in that the dried jujube cheese is obtained by inoculating fresh jujube fruits with mixed bacteria, fermentation and drying; specifically, the following steps are adopted: after the fresh jujube fruits are washed, they are pre-treated without peeling, pitting and puncturing. , add citric acid with a concentration of 0.5% for color protection treatment for 10 minutes; put the processed jujube fruit into a 15×25cm vacuum bag, inoculate the mixed bacterial suspension according to 4% of the weight of the jujube fruit, vacuum seal, and seal at 37±1℃ Ferment for 60-84 hours; bake the fermented jujube fruit at 55-60°C for 10-12 hours until the moisture content is 20%, then vacuum-pack and sterilize it in a normal pressure water bath at 80°C for 20 minutes to obtain a new product of dried date cheese; The mixed bacterial suspension is obtained according to the following steps: Dissolve yeast: bifidobacteria in warm water at 30±0.5°C according to a mass ratio of 1:1 to prepare a suspension with a concentration of 20%, and activate it for 30 minutes. 2.如权利要求1所述的枣酪干,其特征在于,所述枣果为新鲜成熟骏枣、灰枣、哈密大枣中的任意一种。2. Dried jujube cheese as claimed in claim 1, characterized in that the jujube fruit is any one of fresh and mature jujube, gray jujube and Hami jujube.
CN202210392434.5A 2022-04-14 2022-04-14 Fermented dried date cheese and preparation method thereof Active CN114947118B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210392434.5A CN114947118B (en) 2022-04-14 2022-04-14 Fermented dried date cheese and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210392434.5A CN114947118B (en) 2022-04-14 2022-04-14 Fermented dried date cheese and preparation method thereof

Publications (2)

Publication Number Publication Date
CN114947118A CN114947118A (en) 2022-08-30
CN114947118B true CN114947118B (en) 2024-01-16

Family

ID=82977896

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210392434.5A Active CN114947118B (en) 2022-04-14 2022-04-14 Fermented dried date cheese and preparation method thereof

Country Status (1)

Country Link
CN (1) CN114947118B (en)

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101919546A (en) * 2009-06-11 2010-12-22 天津市恒安食品有限公司 Jujube cheese and preparation process thereof
CN102599446A (en) * 2012-04-12 2012-07-25 天津农学院 Low-sugar nutritional jujube powder and preparation method thereof
CN103416686A (en) * 2012-05-15 2013-12-04 周鑫 Solid fermentation jujube
KR20140030831A (en) * 2012-09-04 2014-03-12 김동환 Method for preparing the fermented jujube and it's product
CN105380161A (en) * 2015-10-22 2016-03-09 山东农业大学 Method for producing black dates in solid-state-fermentation mode through red dates
CN107811105A (en) * 2017-12-05 2018-03-20 山西青兆农业科技有限公司 A kind of preparation method of rock sugar red jujube preserved fruit
CN111134296A (en) * 2019-12-26 2020-05-12 山东省食品发酵工业研究设计院 Method for producing low-sugar fermented crisp dates by using whole red dates
KR20200059901A (en) * 2018-11-22 2020-05-29 박호근 manufacturing method of a jujube using lactic acid bacteria
WO2020111724A1 (en) * 2018-11-30 2020-06-04 계명대학교 산학협력단 Fermented jujube having increased content of gaba by using complex fermentation and preparation method therefor
CN111227149A (en) * 2020-02-07 2020-06-05 中国农业大学 A low-sugar fermented red jujube pulp drink rich in polyphenols and preparation method thereof

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101919546A (en) * 2009-06-11 2010-12-22 天津市恒安食品有限公司 Jujube cheese and preparation process thereof
CN102599446A (en) * 2012-04-12 2012-07-25 天津农学院 Low-sugar nutritional jujube powder and preparation method thereof
CN103416686A (en) * 2012-05-15 2013-12-04 周鑫 Solid fermentation jujube
KR20140030831A (en) * 2012-09-04 2014-03-12 김동환 Method for preparing the fermented jujube and it's product
CN105380161A (en) * 2015-10-22 2016-03-09 山东农业大学 Method for producing black dates in solid-state-fermentation mode through red dates
CN107811105A (en) * 2017-12-05 2018-03-20 山西青兆农业科技有限公司 A kind of preparation method of rock sugar red jujube preserved fruit
KR20200059901A (en) * 2018-11-22 2020-05-29 박호근 manufacturing method of a jujube using lactic acid bacteria
WO2020111724A1 (en) * 2018-11-30 2020-06-04 계명대학교 산학협력단 Fermented jujube having increased content of gaba by using complex fermentation and preparation method therefor
CN111134296A (en) * 2019-12-26 2020-05-12 山东省食品发酵工业研究设计院 Method for producing low-sugar fermented crisp dates by using whole red dates
CN111227149A (en) * 2020-02-07 2020-06-05 中国农业大学 A low-sugar fermented red jujube pulp drink rich in polyphenols and preparation method thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Pretreatment methods affecting the color, flavor, bioactive compounds, and antioxidant activity of jujube wine;Wenchao Cai et al.;Food Science & Nutrition;第4965–4975页 *
不同处理方法对金丝2 号枣果实芳香物质的影响;王淑贞等;山东农业科学;第64-67页 *
发酵法制备低糖枣粉可行性研究;樊秀花等;食品科技(04);第104-106页 *
复合菌剂固态发酵对枣粉营养品质的影响;邢晓南等;动物营养学报;第1-11页 *

Also Published As

Publication number Publication date
CN114947118A (en) 2022-08-30

Similar Documents

Publication Publication Date Title
Akubor et al. Production and quality evaluation of banana wine
CN109439489B (en) Preparation method of dragon fruit wine
CN106047564B (en) A kind of brewing method of pomelo wine
CN102356903B (en) A kind of purple sweet potato blackberry compound pulp drink and preparation method thereof
CN110236046A (en) A kind of highly active bacteria type lactic acid bacteria fermented blueberry pomace beverage and preparation method thereof
JP2021136984A (en) Fruits, vegetables and beverage products fermented with probiotics
CN112143607B (en) Preparation method of low-temperature aroma recovery of Nanguoli brandy
CN1759754A (en) Composite juice of lotus root and preparation method
CN1302732C (en) Organe fruit vinegar drink and its producing method
CN111117926B (en) A high-yielding γ-aminobutyric acid strain and its application for preparing γ-aminobutyric acid-rich juice
CN111165798A (en) Preparation method of composite fruit and vegetable enzyme and composite fruit and vegetable enzyme
CN112410162B (en) Hawthorn polyphenol fermented fruit vinegar beverage and preparation process thereof
CN114231381A (en) Preparation method of mixed-strain compound fermented roxburgh rose fruit vinegar
CN114831198B (en) Method for increasing organic acid in Kangpu tea by adding milk permeate powder
CN105802827A (en) Method for producing hawthorn vinegar by hawthorn sugary liquor
CN108865910B (en) Saccharomyces cerevisiae, screening method thereof and application of saccharomyces cerevisiae in blueberry red wine fermentation
KR20080067539A (en) Manufacturing method of vinegar
KR101042576B1 (en) Fermented beverage manufacturing method using pear
CN114947118B (en) Fermented dried date cheese and preparation method thereof
CN113412893A (en) Preparation method of low-sugar high-fiber pear juice beverage
CN105581218B (en) A method for improving the flavor quality of wild blueberry juice by utilizing Lactobacillus plantarum fermentation
CN111534405A (en) Chenopodium quinoa willd wine and method thereof
CN114009768B (en) Daylily probiotic fermented powder, preparation method and fermented daylily solid beverage thereof
CN113729186B (en) Fermented longan pulp and preparation method thereof
CN105995328A (en) Production method of natural cucumis metuliferus cloudy juice enzyme beverage

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant