CN114917259A - Two-dimensional hydrosilylene-microorganism composite material and preparation method and application thereof - Google Patents

Abstract

The inventionProviding a two-dimensional hydrosilylene-microbial composite comprising: microbial carrier and two-dimensional hydrogen-bonded silylene nanosheets loaded on the microbial carrier, wherein the total amount of the two-dimensional hydrogen-bonded silylene nanosheets loaded on each microbial carrier is 10 ^–6 ‑10 ^–11 μ g. The invention also provides a preparation method of the two-dimensional hydrosilacene-microorganism composite material. The invention also provides application of the two-dimensional hydrosilylene-microbial composite material as an intestinal delivery carrier and a free radical scavenger in preparation of medicines for treating inflammatory bowel diseases. The two-dimensional hydrosilacene-microorganism composite material provided by the invention has excellent stability under the acidic condition of the stomach, can be effectively coated on the surface of the microorganism for a long time, protects the microorganism from the attack of gastric acid and enzyme, and greatly improves the survival time and survival rate of the microorganism in the stomach.

Description

A kind of two-dimensional hydrosilene-microorganism composite material and its preparation method and application

technical field

The invention belongs to the technical field of biological nanomaterials, and in particular relates to a two-dimensional hydrosilene-microbial composite material with intestinal microenvironment response characteristics, a preparation method thereof, and the related aspects of intestinal delivery of probiotics and treatment of inflammatory bowel disease. application.

Background technique

The human gut microbiota is composed of numerous bacteria and other microorganisms, has very complex physiological functions, and is closely related to the occurrence and development of various diseases (Science 2018, 362, 776-780). How to regulate the intestinal flora in a safe and effective way to achieve the treatment of diseases is one of the issues of widespread concern. Probiotic therapy is a research hotspot in the field in recent years. Compared with traditional treatment methods, probiotic therapy has the advantages of high safety and resistance to drug resistance, and can assist in the reconstruction of intestinal flora homeostasis, which can play a therapeutic effect on a variety of diseases. Among them, the treatment of enteritis has also received extensive attention (Nat. Rev. Gastroenterol. Hepatol. 2019, 16, 605-616). Probiotic therapy can not only have a beneficial effect on the intestinal epithelium through direct or indirect means, but also can further multiply in the intestine to improve the intestinal flora homeostasis, thus playing a long-term therapeutic role. However, probiotics still face many difficulties in practical use. The most important point is that probiotics are easily inactivated by the acidic conditions of the stomach and the impact of enzymes, and it is difficult to successfully reach the intestinal tract for colonization and play a therapeutic role.

Therefore, it is urgent to develop an efficient microbial intestinal delivery strategy, which can effectively protect microorganisms in the extreme environment of the stomach, promote the intestinal colonization of probiotics, and improve the therapeutic effect of enteritis.

SUMMARY OF THE INVENTION

In view of the limitations of the prior art, the present invention aims to provide a two-dimensional hydrosilene-microorganism composite material with intestinal microenvironment responsive properties and a preparation method thereof, as well as in the intestinal delivery of probiotics and the treatment of inflammatory bowel disease. The related application of , solves the problems of low survival rate of microorganisms in the stomach, insufficient intestinal colonization efficiency and limited therapeutic effect.

In a first aspect, the present invention provides a two-dimensional hydrogen silene-microbial composite material, comprising: a microbial carrier and two-dimensional hydrogen-bonded silicene nanosheets supported on the microbial carrier, wherein each microbial carrier supports a The total amount of the two-dimensional hydrogen-bonded silicene nanosheets is 10 ^-6 -10 ^-11 μg.

Preferably, the microbial carrier is fungi or bacteria, preferably probiotics, including yeast, Trichoderma reesei, Escherichia coli, Bifidobacterium, Lactobacillus, Bacillus subtilis, Staphylococcus aureus and Proteus. One or more, preferably Escherichia coli Nissel 1917 subtype.

Preferably, the two-dimensional hydrogen-bonded silicene nanosheet has a layered sheet structure, the lateral dimension is preferably 300-500 nm, and the thickness is preferably 0.5-10 nm.

Preferably, a polymer surface modifier for promoting the loading of the two-dimensional hydrogen-bonded silicene nanosheet is also included.

Preferably, the polymer surface modifier is a positively charged amphiphilic polymer, preferably vitamin E-polyethylene glycol-polyethyleneimine.

Preferably, in the vitamin E-polyethylene glycol-polyethyleneimine, the molecular weight of polyethylene glycol is 500-10000, preferably 2000; the molecular weight of polyethyleneimine is 400-10000, preferably 600 .

In a second aspect, the present invention also provides a preparation method of the above-mentioned two-dimensional hydrosilene-microbial composite material, comprising the following steps:

Step (1), providing two-dimensional hydrogen-bonded silicene nanosheets;

In step (2), the two-dimensional hydrogen-bonded silicene nanosheets and the polymer modifier are mixed in a solvent, and then the solvent is blown dry with an inert gas, the product is dispersed in water, and centrifuged for cleaning;

In step (3), the product obtained in step (2) is co-incubated with the microbial carrier at low temperature, centrifuged and washed to obtain the two-dimensional hydrosilene-microbial composite material.

Preferably, in step (1), the preparation method of the two-dimensional hydrogen-bonded silicene nanomaterial includes the following steps: immersing the CaSi ₂ powder in a concentrated hydrochloric acid solution, stirring and mixing at -30°C to -10°C , centrifuging, removing the supernatant, and then washing the centrifugation product to obtain the two-dimensional hydrogen-bonded silicene nanomaterial.

Preferably, the particle size of the CaSi ₂ powder is 5-10 μm, and the concentration of the CaSi ₂ is 5-20 mg/mL; stirring and mixing at -30 ℃～-10 ℃ is magnetic stirring, and the magnetic stirring The rotating speed of the magnetron is 500-1000 rpm/molecule, the time of the magnetic stirring is 3-10 days, and the magnetic stirring and mixing are carried out under the protection of an inert gas, and the inert gas is preferably argon gas; the rotating speed of the centrifugal treatment is 13000rpm～20000rpm, the time is 15～20 minutes; the solution used in the washing is absolute ethanol or deionized water, and the number of times of the washing is 3～4 times; it also includes washing the centrifuged products in anhydrous. The step of ultrasonication is carried out in ethanol, the power of the ultrasonication is 500-800W, and the time of the ultrasonication is 8-15h.

Preferably, in step (2), the solvent is selected from one or more of water, ethanol, acetone and chloroform, preferably absolute ethanol, the inert gas is nitrogen; the polymer modifier is Vitamin E-polyethylene glycol-polyethyleneimine, the mass ratio of the vitamin E-polyethylene glycol-polyethyleneimine to the two-dimensional hydrogen-bonded silicene nanosheet is preferably (1-20): 1.

Preferably, in step (3), the concentration of the microbial carrier is 10 ⁶ -10 ¹² CFU/mL, and the concentration of the two-dimensional hydrogen-bonded silicene nanosheets modified by the polymer modifier is 5-200 μg/mL , the co-incubation temperature is 0-10 °C, preferably 4 °C.

In a third aspect, the present invention also provides the application of the above two-dimensional hydrosilene-microorganism composite material as an intestinal delivery carrier and a free radical scavenger for preparing a medicine for treating inflammatory bowel disease.

According to the present invention, the present invention relates to a two-dimensional hydrogen-bonded silicene-microbial composite material, wherein two-dimensional hydrogen-bonded silicene nanosheets are loaded on a probiotic microorganism carrier, and the probiotics are wrapped by the two-dimensional hydrogen-bonded silicene nanosheets. The bacterial microorganism carrier can protect the probiotic microorganism from the supply of gastric acid and enzymes, and improve the survival time and survival rate of the probiotic microorganism in the stomach. The probiotic microorganisms encapsulated by the two-dimensional hydrogen-bonded silicene nanosheets have good microenvironmental response characteristics. The two-dimensional hydrogen-bonded silicene-microorganism composites can rapidly degrade and release the encapsulated probiotic microorganisms after reaching the intestine. It does not affect the metabolism and proliferation of microorganisms, and greatly improves the intestinal colonization efficiency of microorganisms. In the present invention, the two-dimensional hydrogen-bonded silicene-microbial composite material also includes a polymer surface modifier for promoting the loading of the two-dimensional hydrogen-bonded silicene nanosheets, so as to further improve the loading of the two-dimensional hydrogen-bonded silicene nanosheets. The loading rate on the probiotic microorganism carrier, and vitamin E-polyethylene glycol-polyethyleneimine is preferred as a polymer surface modifier to ensure that the modification process will not lead to a decrease in microbial activity.

Beneficial effects of the present invention:

The two-dimensional hydrosilene-microbial composite material provided by the invention and the preparation method thereof have mild preparation conditions, simple and convenient operation, and the modification process will not lead to the decrease of the microbial activity; the two-dimensional hydrosilene-microbial composite material provided by the present invention It has excellent stability under the acidic condition of the stomach, can effectively wrap the microorganism on the surface of the microorganism for a long time, protect the microorganism from the attack of gastric acid and enzymes, and greatly improve the survival time and survival rate of the microorganism in the stomach; The two-dimensional hydrosilene-microorganism composite material has good microenvironmental response characteristics. After reaching the intestine, it can rapidly degrade and release the microbes encapsulated inside, without affecting the metabolism and proliferation of microbes, and greatly improving the intestinal colonization efficiency of microbes. .

Description of drawings

FIG. 1 shows a flow chart for preparing a two-dimensional hydrosilene-microbial composite material according to an embodiment of the present invention.

Figure 2 shows the two-dimensional hydrogen silene nanosheets (SiH) prepared according to Example 1 of the present invention and the hydrogen silene modified with vitamin E-polyethylene glycol-polyethyleneimine (SiH@TPGS-PEI) Morphology of nanosheets under transmission electron microscopy.

FIG. 3 shows a scanning electron microscope image of the two-dimensional hydrosilene-microorganism composite material prepared according to Example 1 of the present invention.

FIG. 4 shows a graph showing the change of ultraviolet-visible absorption spectrum with time of the hydrosilene nanosheets modified with vitamin E-polyethylene glycol-polyethyleneimine in artificial gastric juice according to Example 2 of the present invention.

FIG. 5 shows a graph showing the change of ultraviolet-visible absorption spectrum with time of the hydrosilene nanosheets modified with vitamin E-polyethylene glycol-polyethyleneimine in artificial intestinal fluid according to Example 2 of the present invention.

FIG. 6 shows the bacterial survival of the two-dimensional hydrosilene-microorganism composite material in artificial gastric juice according to Example 3 of the present invention.

FIG. 7 shows the evaluation chart of the survival time of the two-dimensional hydrosilene-microorganism composite material in artificial gastric juice according to Example 4 of the present invention.

Detailed ways

The present invention will be further described below with reference to the accompanying drawings and the following embodiments. It should be understood that the accompanying drawings and the following embodiments are only used to illustrate the present invention, but not to limit the present invention.

According to a first aspect of the present invention, a two-dimensional hydrogen silene-microorganism composite material is provided, comprising: a microbial carrier and a two-dimensional hydrogen-bonded silicene nanosheet supported on the microbial carrier, optionally further A polymer surface modifier for promoting the loading of the two-dimensional hydrogen-bonded silicene nanosheet is included.

According to the present invention, the microbial carrier is a fungus or a bacterium, and in the embodiment of the present invention is a probiotic, including yeast, Trichoderma reesei, Escherichia coli, Bifidobacterium, Lactobacillus, Bacillus subtilis, and Staphylococcus aureus and one or more of Proteus, in the embodiment of the present invention, is Escherichia coli Nissel 1917 subtype.

Probiotics are fungi or bacteria that are beneficial to the human body or animals. Probiotics are colonized in the human body and change the composition of a certain part of the host's flora. A kind of active microorganisms that are beneficial to the host. By regulating the immune function of the host mucosa and system or by regulating the balance of intestinal flora, promoting nutrient absorption and maintaining intestinal health, resulting in the production of single microorganisms or mixed microorganisms with a clear composition that are beneficial to health.

However, probiotics still face many difficulties in practical use. The most important point is that probiotics are easily inactivated by the acidic conditions of the stomach and the impact of enzymes, and it is difficult to successfully reach the intestinal tract for colonization and play a therapeutic role. Therefore, on the basis of the original probiotic microbial carrier, the present invention also supports two-dimensional hydrogen-bonded silicene nanosheets on the probiotic microbial carrier. The probiotic microorganism carrier encapsulated by two-dimensional hydrogen-bonded silicene nanosheets can protect the probiotic microorganism from the supply of gastric acid and enzymes, and improve the survival time and survival rate of the probiotic microorganism in the stomach. The probiotic microorganisms encapsulated by the two-dimensional hydrogen-bonded silicene nanosheets have good microenvironmental response characteristics. The two-dimensional hydrogen-bonded silicene-microorganism composites can rapidly degrade and release the encapsulated probiotic microorganisms after reaching the intestine. It does not affect the metabolism and proliferation of microorganisms, and greatly improves the intestinal colonization efficiency of microorganisms.

According to the present invention, the two-dimensional hydrogen-bonded silicene nanosheet is a layered sheet structure, the lateral dimension is preferably 300-500 nm, and the thickness is preferably 0.5-10 nm.

In order to promote the loading of the two-dimensional hydrogen-bonded silicene nanosheets, the present invention also optionally includes a polymer surface modifier for modifying the surface of the two-dimensional hydrogen-bonded silicene nanosheets. The polymer surface modifier is a positively charged amphiphilic polymer, and in the embodiment of the present invention, vitamin E-polyethylene glycol-polyethyleneimine is used.

The two-dimensional hydrogen-bonded silicene-microbial composite material according to the present invention also includes a high-efficiency method for modifying the surface of the two-dimensional hydrogen-bonded silicene nanosheets to facilitate the loading of the two-dimensional hydrogen-bonded silicene nanosheets on the probiotic microorganism carrier. Molecular surface modifier to further improve the loading rate of two-dimensional hydrogen-bonded silicene nanosheets on probiotic microbial carriers. At the same time, vitamin E-polyethylene glycol-polyethyleneimine is preferred as a polymer surface modifier to ensure modification The process does not result in a decrease in microbial activity.

In a preferred embodiment, in the vitamin E-polyethylene glycol-polyethyleneimine, the molecular weight of polyethylene glycol is 500-10000, such as 2000; the molecular weight of polyethyleneimine is 400-10000, such as 600.

The present invention also provides a preparation method of the above-mentioned two-dimensional hydrosilene-microorganism composite material. The preparation method is mainly divided into three parts, including the preparation of hydrosilene nanosheets, the modification of hydrosilene nanosheets, and the assembly of modified hydrosilene nanosheets with microorganisms. The specific preparation method includes the following steps:

Step 1, preparation of two-dimensional hydrosilene nanosheets: immerse the CaSi ₂ powder in a concentrated hydrochloric acid solution, stir and mix magnetically at -30°C to -10°C under the protection of inert gas, centrifuge and remove the supernatant , and then the centrifugation product is washed to obtain the two-dimensional hydrogen-bonded silicene nanomaterial.

In this step 1, the particle size of the CaSi ₂ powder is 5-10 μm, and the concentration is 5-20 mg/mL, for example, 10 mg/mL; the magnetic stirring speed of the magnetic stirring is 500-1000 rpm/molecule, and the time It is 3-10 days, for example, 7 days; the inert gas is preferably argon; the rotational speed of the centrifugal treatment is 13000rpm～20000rpm, and the time is 15～20 minutes; the solution used in the washing is absolute ethanol or dehydrated Ionized water, and the washing times are 3 to 4 times.

In the preparation process of the two-dimensional hydrosilene nanosheets, it also includes the step of ultrasonically crushing the material after washing the centrifuged product in anhydrous ethanol, the power of ultrasonication is 500-800W, and the time of ultrasonication is 8 -15h.

Step 2, modification of two-dimensional hydrogen-bonded silicene nanosheets: Mix two-dimensional hydrogen-bonded silicene nanosheets with a polymer modifier in a solvent, then dry the solvent with an inert gas, disperse the product in water, and wash by centrifugation.

In this step 2, the solvent is selected from one or more of water, ethanol, acetone and chloroform, preferably absolute ethanol, and the inert gas is nitrogen; in the embodiment of the present invention, the polymer The modifier is vitamin E-polyethylene glycol-polyethyleneimine, and the mass ratio of the vitamin E-polyethylene glycol-polyethyleneimine to the two-dimensional hydrogen-bonded silicene nanosheet is preferably (1- 20):1, eg 10:1.

Step 3, the assembly of the modified two-dimensional hydrosilene nanosheet and the probiotic microbial carrier: co-incubate the product obtained in step 2 with the probiotic microbial carrier at a low temperature of, for example, 4°C, centrifuge and wash to obtain the two-dimensional Vitamin Hydrosilene-Microbial Composites.

In this step 3, the concentration of the microbial carrier is 10 ⁶ -10 ¹² CFU/mL, for example, 10 ⁸ CFU/mL; the concentration of the two-dimensional hydrogen-bonded silicene nanosheets modified by the polymer modifier is 5- 200 μg/mL, such as 30 μg/mL.

The present invention also provides the application of the above two-dimensional hydrosilene-microorganism composite material as an intestinal delivery carrier and a free radical scavenger for preparing a medicine for treating inflammatory bowel disease. According to the present invention, the two-dimensional hydrosilene-microorganism composite material provided by the present invention has excellent stability under the acidic conditions of the stomach, and can be effectively wrapped on the surface of microorganisms for a long time, protect the microorganisms from the attack of gastric acid and enzymes, and greatly improve the stability of the microorganisms. The survival time and survival rate of microorganisms in the stomach are improved; the two-dimensional hydrosilene-microorganism composite material provided by the present invention has good microenvironment response characteristics, and can rapidly degrade and release the microorganisms encapsulated inside after reaching the intestinal tract. It does not affect the metabolism and proliferation of microorganisms, and greatly improves the intestinal colonization efficiency of microorganisms.

The following further examples are given to illustrate the present invention in detail. It should also be understood that the following examples are only used to further illustrate the present invention, and should not be construed as limiting the scope of protection of the present invention. Some non-essential improvements and adjustments made by those skilled in the art according to the foregoing content of the present invention belong to the present invention. protected range. The specific process parameters and the like in the following examples are only an example of a suitable range, that is, those skilled in the art can make selections within the suitable range through the descriptions herein, but are not necessarily limited to the specific values exemplified below.

Example 1:

Preparation of two-dimensional hydrosilene-microbial composites: The preparation steps are mainly divided into three parts, including the preparation of hydrosilene nanosheets, the modification of hydrosilene nanosheets, and the modified hydrosilene nanosheets and probiotic microorganisms assembly. The specific preparation process is shown in Figure 1, and the three steps are described below:

Preparation of hydrosilene nanosheets: 1 g of calcium silicide was added to 100 mL of pre-cooled concentrated hydrochloric acid, and vigorously stirred for 7 days at -20°C under argon protection. After the reaction was completed, centrifuge at 12000 rpm for 10 min, and the precipitate was washed with acetone and absolute ethanol in turn, and then resuspended in absolute ethanol. After the ice bath probe was sonicated for 8 hours, the hydrogen silene nanosheets were obtained, and the results of transmission electron microscopy were shown in Figure 2.

Modification of hydrogen silene nanosheets: 1 mg of hydrogen silene nanosheets and 10 mg of vitamin E-polyethylene glycol-polyethyleneimine were co-dispersed in absolute ethanol, dried with nitrogen, and resuspended in ultrapure water, centrifuged After washing twice, vitamin E-polyethylene glycol-polyethyleneimine-modified hydrosilene nanosheets were obtained, and the results of transmission electron microscopy are shown in Figure 2.

Assembly of modified hydrosilene nanosheets with probiotic microorganisms: The modified hydrosilene nanosheets were resuspended in ultrapure water and diluted to different concentrations of hydrosilene suspensions. The probiotic microorganisms were centrifuged at 7000 rpm for 5 min, washed twice and dispersed in ultrapure water. The hydrosilene suspension is mixed with the probiotic microorganism suspension, incubated at 4° C. for 20-30 min, and centrifuged and washed three times to obtain a two-dimensional hydrosilene-microorganism composite material. The scanning electron microscope results are shown in Figure 3.

Example 2:

The vitamin E-polyethylene glycol-polyethyleneimine-modified hydrosilene nanosheets were dispersed in artificial gastric juice and artificial intestinal juice, and the vitamin E-polyethylene glycol was determined by monitoring the changes of its ultraviolet-visible absorption spectrum. Stability and responsiveness of alcohol-polyethyleneimine-modified hydrosilene nanosheets in different physiological environments. It can be seen from Figures 4 and 5 that the material has good stability in artificial gastric juice (Figure 4), and the UV-Vis absorption spectrum has almost no change. In artificial intestinal fluid (Fig. 5), the UV-Vis absorption value decreased rapidly, indicating that the material could be rapidly degraded in artificial intestinal fluid.

Example 3:

Evaluation of bacterial viability of two-dimensional hydrosilene-microbe composites in gastric juice. 1 mL of probiotics in logarithmic growth phase were centrifuged and resuspended in 100 μL of ultrapure water, and mixed with different concentrations of vitamin E-polyethylene glycol-polyethyleneimine-modified hydrosilene nanosheets in sterile water. Mixed, the final hydrosilene content was 0, 3, 15 and 30 μg. After co-incubating for half an hour, the two-dimensional hydrosilene-probiotic composite material was obtained by centrifugation and washing. The obtained composite materials were dispersed in artificial gastric juice, and the survival rate was counted by plate coating method after incubation for 15 min. As shown in Figure 6, the unmodified probiotics have no bacterial survival after interacting with gastric juice, while the hydrosilene-probiotic composite material exhibits a good probiotic protection effect, which greatly improves the survival of probiotics in gastric juice. Rate.

Example 4:

Evaluation of survival time of two-dimensional hydrosilene-microbial composites in gastric juice. 1 mL of probiotics in the logarithmic growth phase were centrifuged, resuspended in 100 μL of ultrapure water, and mixed with vitamin E-polyethylene glycol-polyethyleneimine-modified hydrosilene nanosheets in sterile water. The content of hydrosilene was 30 μg. After co-incubating for half an hour, the two-dimensional hydrosilene-probiotic composite material was obtained by centrifugation and washing. The obtained composite material was dispersed in 1 mL of artificial gastric juice, and the survival rate was counted by the plate coating method after incubation for 15 min, 30 min and 60 min. The survival efficiency of probiotics is greatly improved in time.

Industrial applicability:

The two-dimensional hydrosilene-microbial composite material provided by the invention and the preparation method thereof have mild preparation conditions, simple and convenient operation, and the modification process will not lead to the decrease of the microbial activity; the two-dimensional hydrosilene-microbial composite material provided by the present invention It has excellent stability under the acidic condition of the stomach, can effectively wrap the microorganism on the surface of the microorganism for a long time, protect the microorganism from the attack of gastric acid and enzymes, and greatly improve the survival time and survival rate of the microorganism in the stomach; The two-dimensional hydrosilene-microorganism composite material has good microenvironmental response characteristics. After reaching the intestine, it can rapidly degrade and release the microbes encapsulated inside, without affecting the metabolism and proliferation of microbes, and greatly improving the intestinal colonization efficiency of microbes. .

Claims (12)

1. A two-dimensional hydrosilylene-microbial composite, comprising: microbial carrier and two-dimensional hydrogen-bonded silylene nanosheets loaded on the microbial carrier, wherein the total amount of the two-dimensional hydrogen-bonded silylene nanosheets loaded on each microbial carrier is 10 ^–6 -10 ^–11 μg。

2. The two-dimensional hydrosilylene-microbe composite material of claim 1, wherein the microbe carrier is fungus or bacteria, preferably probiotic bacteria, including one or more of yeast, trichoderma reesei, escherichia coli, bifidobacterium, lactobacillus, bacillus subtilis, staphylococcus aureus and proteus, preferably escherichia coli, Nissel 1917 subtype.

3. The two-dimensional hydrosilylene-microbial composite material as claimed in claim 1 or 2, wherein the two-dimensional hydrogen bonded silylene nanosheet is of a layered lamellar structure, the transverse dimension is preferably 300-500 nm, and the thickness is preferably 0.5-10 nm.

4. The two-dimensional hydrosilylene-microbial composite of any of claims 1-3 further comprising a polymeric surface modifier for promoting the loading of the two-dimensional hydrogen bonded silylene nanoplatelets.

5. The two-dimensional hydrosilylene-microbial composite of claim 4, wherein the polymeric surface modifier is a positively charged amphiphilic polymer, preferably vitamin E-polyethylene glycol-polyethyleneimine.

6. The two-dimensional hydrosilylene-microbe composite material as claimed in claim 5, wherein in the vitamin E-PEG-polyethyleneimine, the molecular weight of PEG is 500-10000, preferably 2000; the molecular weight of polyethyleneimine is 400-10000, preferably 600.

7. A method for preparing a two-dimensional hydrosilylene-microbial composite as claimed in any of claims 1-6, comprising the steps of:

step (1), providing a two-dimensional hydrogen bonding silicon alkene nano sheet;

mixing the two-dimensional hydrogen bonded silylene nanosheets and the high molecular modifier in a solvent, then blowing the solvent dry by using inert gas, dispersing the product in water, and centrifugally cleaning;

and (3) co-incubating the product obtained in the step (2) and a microbial carrier at a low temperature, centrifuging and washing to obtain the two-dimensional hydrosilylene-microbial composite material.

8. The preparation method according to claim 7, wherein in the step (1), the preparation method of the two-dimensional hydrogen-bonded silylene nanomaterial comprises the following steps: mixing CaSi ₂ And immersing the powder into concentrated hydrochloric acid solution, stirring and mixing at-30 to-10 ℃, centrifuging, removing supernatant, and washing a centrifugal product to obtain the two-dimensional hydrogen bonded silylene nano material.

9. The method according to claim 8, wherein the CaSi is used as a base material ₂ The particle size of the powder is 5-10 mu m, and the CaSi is ₂ The concentration of (a) is 5-20 mg/mL; stirring and mixing at-30 to-10 ℃ to form magnetic stirring, wherein the rotating speed of magnetons of the magnetic stirring is 500 to 1000 revolutions per molecule, the magnetic stirring time is 3 to 10 days, and the magnetic stirring and mixing are carried out under the protection of inert gas, wherein the inert gas is preferably argon; the rotation speed of the centrifugal treatment is 13000rpm to 20000rpm, and the time is 15 minutes to 20 minutes; the solution adopted by washing is absolute ethyl alcohol or deionized water, and the washing times are 3-4 times; the method further comprises the step of carrying out ultrasonic crushing on the material after the centrifugal product is washed in absolute ethyl alcohol, wherein the power of the ultrasonic crushing is 500-800W, and the time of the ultrasonic crushing is 8-15 h.

10. The preparation method according to claim 7, wherein in the step (2), the solvent is one or more selected from water, ethanol, acetone and chloroform, preferably absolute ethanol, and the inert gas is nitrogen; the high-molecular modifier is vitamin E-polyethylene glycol-polyethyleneimine, and the mass ratio of the vitamin E-polyethylene glycol-polyethyleneimine to the two-dimensional hydrogen bonded silylene nanosheet is preferably (1-20): 1.

11. the method according to claim 7, wherein the concentration of the microorganism carrier in the step (3) is 10 ⁶ -10 ¹² CFU/mL, the concentration of the two-dimensional hydrogen bonding silicon alkene nano-sheet modified by the macromolecular modifier is 5-200 mug/mL, and the co-incubation temperatureIs 0 to 10 ℃ and preferably 4 ℃.

12. Use of the two-dimensional hydrosilylene-microbial composite of any of claims 1-6 as an intestinal delivery vehicle and a free radical scavenger for the manufacture of a medicament for the treatment of inflammatory bowel disease.

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