CN114869839B - Multilayer microencapsulated mild composite acid repair gel based on lactobacillus fermentation product EPS, and preparation method and application thereof - Google Patents
Multilayer microencapsulated mild composite acid repair gel based on lactobacillus fermentation product EPS, and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a multilayer microencapsulated mild composite acid repair gel based on a lactobacillus fermentation product EPS, a preparation method and application thereof. The composite acid repair gel comprises the following components: lactobacillus fermented extract, salicylic acid, octanoylsalicylic acid, glycollic acid, mandelic acid, citric acid, and lactic acid. The preparation method of the lactobacillus fermentation extract comprises the following steps: inoculating lactobacillus seed liquid into a fermentation medium for culture to obtain lactobacillus fermentation liquid; the fermentation medium contains threonine and serine. The mild repairing gel prepared by adopting a 1+N formula and an exclusive multilayer microcapsule wrapping technology can solve the problem of irritation and dissolution of salicylic acid and octanoyl salicylic acid, can slowly and uniformly release active ingredients, acts on each layer of skin more accurately, plays roles of removing acnes and repairing skin, and has effectiveness and safety.
Description
Technical Field
The invention belongs to the technical field of skin care products, and particularly relates to a multilayer microencapsulated mild composite acid repair gel based on a lactobacillus fermentation product EPS, a preparation method and application thereof.
Background
The compound acid is a novel chemical skin-changing agent prepared by compounding two or more than two kinds of single acids with different action mechanisms through a special process, and can be prepared into gel and other dosage forms. Through reasonable compatibility and preparation process, different monoacid action mechanisms can mutually compensate (1+1 > 2) and permeate to different skin layers, and the monoacid can act on the skin in multiple targets to enhance the curative effect; greatly reduces the concentration of the single acid, improves the safety and reduces the side effect. At present, a plurality of compound acid products are available on the market, but the quality is uneven, and the effect is difficult to satisfy consumers.
Lactic acid bacteria exopolysaccharides (Exopoly Saccharides, EPS) are a class of carbohydrate compounds secreted out of the cell wall by lactic acid bacteria during the growth metabolism. The extracellular polysaccharide produced by different lactic acid bacteria and under different fermentation conditions is considered to have different structural and biological activities. Part of the lactobacillus extracellular polysaccharide has good biological activity (such as immunological activity, anti-tumor and anti-ulcer). At present, the precedent of compounding specific lactobacillus extracellular polysaccharide with different monoacids to prepare a chemical skin-changing agent is not found.
Disclosure of Invention
In view of the defects of the prior art, the invention provides a multilayer microencapsulated mild composite acid repair gel based on a lactobacillus fermentation product EPS, and a preparation method and application thereof.
The technical scheme of the invention mainly comprises the following contents:
the multi-layer microencapsulated mild composite acid repair gel based on the lactobacillus fermentation product EPS comprises the following components in parts by weight: lactobacillus fermented extract, salicylic acid, octanoylsalicylic acid, glycollic acid, mandelic acid, citric acid, lactic acid and emulsifier. The preparation method of the lactobacillus fermentation extract comprises the following steps: inoculating the lactobacillus seed solution into a fermentation medium for culture to obtain lactobacillus fermentation liquid. The fermentation medium contains threonine and serine.
Preferably, the lactic acid bacteria comprise lactobacillus plantarum or lactobacillus brevis.
Preferably, the lactobacillus fermentation extract comprises extracellular polysaccharide.
Preferably, the fermentation medium contains 0.02-0.05 g/L threonine and 0.02-0.05 g/L serine.
The culture medium is a complex system, and different components and interactions among the components can influence the growth metabolism of microorganisms. Threonine and serine are added into a common MRS culture medium as induction factors, so that the generation of active polysaccharide is promoted. By means of the fermentation, the content of extracellular polysaccharide of lactobacillus plantarum is not the highest, but the activity is the highest, and the acne removing and repairing effects are the best.
Preferably, the multi-layer microencapsulated mild complex acid repair gel based on the lactobacillus fermentation product EPS comprises the following components in parts by weight: 20-27 parts of lactobacillus fermentation extract, 15-20 parts of salicylic acid, 1-3 parts of octanoyl salicylic acid, 1-2 parts of glycollic acid, 3-5 parts of mandelic acid, 1-2 parts of citric acid, 1-3 parts of lactic acid and an emulsifying agent.
Preferably, the preparation method of the lactobacillus fermentation extract comprises the following steps: inoculating lactobacillus seed liquid to MRS culture medium containing 0.02-0.05 g/L threonine, fermenting and culturing for 24-36 h, adding 0.02-0.05 g/L serine into the fermentation culture medium, and continuing fermenting and culturing for 24-36 h to obtain lactobacillus fermentation liquid; centrifuging lactobacillus fermentation liquor, taking supernatant, collecting components with molecular weight of 15000-30000 Da, and drying to obtain lactobacillus fermentation extract.
Preferably, the lactobacillus fermentation broth is centrifuged, the supernatant is taken, the first component with the molecular weight between 5000 and 15000Da and the second component with the molecular weight between 15000 and 30000Da are respectively collected, and the mixture is dried for standby; the second component is lactobacillus fermentation extract in the formula; the addition amount of the first component in the formula is 0-5 parts.
The first component contains partial low molecular weight lipopeptides, organic acids, polysaccharides and other components, has an emulsifying effect, plays a role in promoting bacteriostasis to a certain extent, can be added into a formula, realizes full utilization of fermentation products, and saves production cost.
The invention also provides a preparation method of the multi-layer microencapsulated mild composite acid repair gel based on the lactobacillus fermentation product EPS, which comprises the following steps:
(1) Adding aqueous solution into lactobacillus fermentation extract to obtain phase A;
(2) Mixing salicylic acid and octanoyl salicylic acid to obtain phase B; slowly adding the phase A into the phase B, and mixing at 45-55 ℃;
(3) Uniformly mixing other components in the formula, and adding water to obtain a phase C;
(4) And slowly dripping the mixture obtained in the step (2) into the phase C, keeping stirring in the dripping process, and continuing stirring after the dripping is finished to obtain the complex acid repairing gel.
On the other hand, the invention also provides the application of the multi-layer microencapsulated mild complex acid repair gel based on the lactobacillus fermentation product EPS in the aspect of preparing skin care products.
MRS medium composition: 10.0g of casein peptone, 10.0g of beef extract, 5.0g of yeast powder, 5.0g of glucose, 5.0g of sodium acetate, 2.0g of diammonium citrate, 1.0g of tween 80 and K 2 HPO 4 2.0g，MgSO 4 .7H 2 O 0.2g，MnSO 4 .H 2 O 0.05g，CaCO 3 20.0g, 15.0g of agar, 1.0L of distilled water and pH6.8.
The invention has the following effects:
the mild repairing gel prepared by adopting a 1+N formula and an exclusive multilayer microcapsule wrapping technology can solve the problem of irritation and dissolution of salicylic acid and octanoyl salicylic acid, can slowly and uniformly release active ingredients, acts on each layer of skin more accurately, plays roles of removing acnes and repairing skin, and has effectiveness and safety.
According to the formula, an organic solvent is not required to be introduced, the dosage of the emulsifier is low, good acne removing and acne removing effects and skin restoration effects can be achieved, the safety of products can be ensured, and meanwhile, the production cost can be effectively controlled.
Drawings
Fig. 1: the product of the embodiment 4 of the invention is provided with a using effect graph. (age: 22, sex: female, skin problems: acne, use cycle: 1 time per week, 3 times in succession).
Detailed Description
For a better understanding of the technical content of the present invention, the present invention will be further described with reference to the following specific examples and the accompanying drawings.
The invention first screens the complex acid formulation, and the following is a part of examples and test results.
EXAMPLE 1 Mild Complex acid repair gel
The formula comprises the following components in parts by weight: 23 parts of lactobacillus fermentation extract, 15 parts of salicylic acid, 1 part of octanoylsalicylic acid, 2 parts of glycollic acid, 3 parts of mandelic acid, 1 part of citric acid, 1 part of lactic acid, 5 parts of tween 80 and 49 parts of water.
The lactobacillus fermentation extract is extracellular polysaccharide separated from traditional dairy products in pasture areas.
EXAMPLE 2 Mild Complex acid repair gel
The formula comprises the following components in parts by weight: 23 parts of lactobacillus fermentation extract, 15 parts of salicylic acid, 1 part of octanoylsalicylic acid, 2 parts of glycollic acid, 3 parts of mandelic acid, 1 part of citric acid, 1 part of lactic acid, 5 parts of tween 80 and 49 parts of water.
The lactobacillus fermentation extract is as follows: inoculating single colony of lactobacillus (Lactobacillus plantarum AS 1.555) into MRS culture medium, activating and culturing at 37deg.C for 24 hr to obtain seed solution, inoculating the seed solution into MRS culture medium according to 4w/w% inoculum size, fermenting and culturing at 37deg.C for 72 hr to obtain lactobacillus fermentation liquid. Centrifuging lactobacillus fermentation liquor at 2000rpm for 10min, collecting supernatant, and collecting components with molecular weight of 15000-30000 Da by membrane separation method to obtain fermented extract, and drying under reduced pressure for use.
EXAMPLE 3 Mild Complex acid repair gel
The formula comprises the following components in parts by weight: 23 parts of lactobacillus fermentation extract, 15 parts of salicylic acid, 1 part of octanoylsalicylic acid, 2 parts of glycollic acid, 3 parts of mandelic acid, 1 part of citric acid, 1 part of lactic acid, 5 parts of tween 80 and 49 parts of water.
The lactobacillus fermentation extract is as follows: inoculating single colony of lactobacillus (Lactobacillus plantarum AS 1.555) into MRS culture medium, activating and culturing at 37deg.C for 24 hr to obtain seed solution, inoculating seed solution into MRS culture medium containing 0.02g/L threonine according to 4w/w% inoculum size, fermenting and culturing at 37deg.C for 72 hr to obtain lactobacillus fermentation broth. Centrifuging lactobacillus fermentation liquor at 2000rpm for 10min, collecting supernatant, and collecting components with molecular weight of 15000-30000 Da by membrane separation method to obtain fermented extract, and drying under reduced pressure for use.
EXAMPLE 4 Mild Complex acid repair gel
The formula comprises the following components in parts by weight: 23 parts of lactobacillus fermentation extract, 15 parts of salicylic acid, 1 part of octanoylsalicylic acid, 2 parts of glycollic acid, 3 parts of mandelic acid, 1 part of citric acid, 1 part of lactic acid, 5 parts of tween 80 and 49 parts of water.
The lactobacillus fermentation extract is as follows: inoculating single colony of lactobacillus (Lactobacillus plantarum AS 1.555) into MRS culture medium, activating and culturing at 37deg.C for 24 hr to obtain seed solution, inoculating seed solution into MRS culture medium containing 0.02g/L threonine according to 4w/w% inoculum size, fermenting and culturing at 37deg.C for 36 hr, adding 0.02g/L serine into fermentation culture medium, and fermenting and culturing for 36 hr to obtain lactobacillus fermentation liquid. Centrifuging lactobacillus fermentation liquor at 2000rpm for 10min, collecting supernatant, collecting components with molecular weight of 15000-30000 Da by membrane separation method, and drying under reduced pressure.
EXAMPLE 5 Mild Complex acid repair gel
The formula comprises the following components in parts by weight: 23 parts of lactobacillus fermentation extract, 15 parts of salicylic acid, 1 part of octanoylsalicylic acid, 2 parts of glycollic acid, 3 parts of mandelic acid, 1 part of citric acid, 1 part of lactic acid, 5 parts of tween 80 and 49 parts of water.
The lactobacillus fermentation extract is as follows: inoculating single colony of lactobacillus (Lactobacillus plantarum AS 1.555) into MRS culture medium, activating and culturing at 37deg.C for 24 hr to obtain seed solution, inoculating the seed solution into MRS culture medium containing 0.02g/L serine according to 4w/w% inoculum size, fermenting and culturing at 37deg.C for 36 hr, adding 0.02g/L threonine into fermentation culture medium, and fermenting and culturing for 36 hr to obtain lactobacillus fermentation liquid. Centrifuging lactobacillus fermentation liquor at 2000rpm for 10min, collecting supernatant, and collecting components with molecular weight of 15000-30000 Da by membrane separation method to obtain fermented extract, and drying under reduced pressure for use.
EXAMPLE 6 Mild Complex acid repair gel
The formula comprises the following components in parts by weight: 23 parts of lactobacillus fermentation extract, 15 parts of salicylic acid, 1 part of octanoylsalicylic acid, 2 parts of glycollic acid, 3 parts of mandelic acid, 1 part of citric acid, 1 part of lactic acid, 5 parts of tween 80 and 49 parts of water.
The lactobacillus fermentation extract is as follows: inoculating single colony of lactobacillus (Lactobacillus plantarum AS 1.555) into MRS culture medium, activating and culturing at 37deg.C for 24 hr to obtain seed solution, inoculating seed solution into MRS culture medium containing 0.05g/L threonine according to 4w/w% inoculum size, fermenting and culturing at 37deg.C for 24 hr, adding 0.05g/L serine into fermentation culture medium, and fermenting and culturing for 24 hr to obtain lactobacillus fermentation liquid. Centrifuging lactobacillus fermentation liquor at 2000rpm for 10min, collecting supernatant, collecting components with molecular weight of 15000-30000 Da by membrane separation method, and drying under reduced pressure.
EXAMPLE 7 Mild Complex acid repair gel
The formula comprises the following components in parts by weight: 23 parts of lactobacillus fermentation extract, 15 parts of salicylic acid, 1 part of octanoylsalicylic acid, 2 parts of glycollic acid, 3 parts of mandelic acid, 1 part of citric acid, 1 part of lactic acid, 5 parts of tween 80 and 49 parts of water.
The lactobacillus fermentation extract is as follows: inoculating single colony of lactobacillus (Lactobacillus brevis, separated from dairy products) into MRS culture medium, activating and culturing at 37deg.C for 24 hr to obtain seed solution, inoculating seed solution into MRS culture medium containing 0.02g/L threonine according to 4w/w% inoculum size, fermenting and culturing at 37deg.C for 36 hr, adding 0.02g/L serine into fermentation culture medium, and fermenting and culturing for 36 hr to obtain lactobacillus fermentation liquid. Centrifuging lactobacillus fermentation liquor at 2000rpm for 10min, collecting supernatant, collecting components with molecular weight of 15000-30000 Da by membrane separation method, and drying under reduced pressure.
The preparation method of the products of examples 1-7:
(1) Adding water into lactobacillus fermented extract to obtain 70w/w% solution to obtain phase A;
(2) Mixing salicylic acid and octanoyl salicylic acid to obtain phase B; slowly adding phase A into phase B, and mixing at 50+ -5deg.C (50 rpm,3 h);
(3) Uniformly mixing glycolic acid, citric acid, mandelic acid, lactic acid and tween 80, and adding the balance of water to obtain a phase C;
(4) And slowly dripping the mixture obtained in the step (2) into the C phase at 25 ℃, keeping stirring at 50rpm in the dripping process, and continuing stirring for 1h after the dripping is finished to obtain the complex acid repairing gel.
EXAMPLE 8 Mild Complex acid repair gel
The formula comprises the following components in parts by weight: 23 parts of lactobacillus fermentation extract, 15 parts of salicylic acid, 1 part of octanoylsalicylic acid, 2 parts of glycollic acid, 3 parts of mandelic acid, 1 part of citric acid, 1 part of lactic acid, 5 parts of tween 80 and 49 parts of water.
The lactobacillus fermentation extract is as follows: inoculating single colony of lactobacillus (Lactobacillus plantarum AS 1.555) into MRS culture medium, activating and culturing at 37deg.C for 24 hr to obtain seed solution, inoculating seed solution into MRS culture medium containing 0.02g/L threonine according to 4w/w% inoculum size, fermenting and culturing at 37deg.C for 36 hr, adding 0.02g/L serine into fermentation culture medium, and fermenting and culturing for 36 hr to obtain lactobacillus fermentation liquid. Centrifuging the lactobacillus fermentation liquid at 2000rpm for 10min, collecting supernatant, and respectively collecting first components with molecular weight of 5000-15000 Da (replacing 2.5 parts of Tween 80 after drying) and second components with molecular weight of 15000-30000 Da (used as lactobacillus fermentation extract in the formula after drying) by membrane separation method.
The preparation method of the composite acid repair gel comprises the following steps:
(1) Adding water into the second component to prepare a 70w/w% solution to obtain a phase A;
(2) Mixing salicylic acid and octanoyl salicylic acid to obtain phase B; slowly adding phase A into phase B, and mixing at 50+ -5deg.C (50 rpm,3 h);
(3) Uniformly mixing the glycolic acid, the citric acid, the mandelic acid, the lactic acid, the tween 80 and the first component, and adding the balance of water to obtain a phase C;
(4) And slowly dripping the mixture obtained in the step (2) into the C phase at 25 ℃, keeping stirring at 50rpm in the dripping process, and continuing stirring for 1h after the dripping is finished to obtain the complex acid repairing gel.
EXAMPLE 9 Mild Complex acid repair gel
The formula comprises the following components in parts by weight: 20 parts of lactobacillus fermentation extract, 17 parts of salicylic acid, 2 parts of octanoylsalicylic acid, 2 parts of glycollic acid, 5 parts of mandelic acid, 1 part of citric acid, 1 part of lactic acid, 5 parts of tween 80 and 47 parts of water.
The lactobacillus fermentation extract is as follows: inoculating single colony of lactobacillus (Lactobacillus plantarum AS 1.555) into MRS culture medium, activating and culturing at 37deg.C for 24 hr to obtain seed solution, inoculating seed solution into MRS culture medium containing 0.02g/L threonine according to 4w/w% inoculum size, fermenting and culturing at 37deg.C for 36 hr, adding 0.02g/L serine into fermentation culture medium, and fermenting and culturing for 36 hr to obtain lactobacillus fermentation liquid. Centrifuging the lactobacillus fermentation liquid at 2000rpm for 10min, collecting supernatant, and respectively collecting first components with molecular weight of 5000-15000 Da (replacing 2.5 parts of Tween 80 after drying) and second components with molecular weight of 15000-30000 Da (used as lactobacillus fermentation extract in the formula after drying) by membrane separation method.
The preparation method of the composite acid repair gel comprises the following steps:
(1) Adding water into the second component to prepare 80w/w% solution to obtain phase A;
(2) Mixing salicylic acid and octanoyl salicylic acid to obtain phase B; slowly adding phase A into phase B, and mixing at 50+ -5deg.C (50 rpm,3 h);
(3) Uniformly mixing the glycolic acid, the citric acid, the mandelic acid, the lactic acid, the tween 80 and the first component, and adding the balance of water to obtain a phase C;
(4) And slowly dripping the mixture obtained in the step (2) into the C phase at 25 ℃, keeping stirring at 50rpm in the dripping process, and continuing stirring for 1h after the dripping is finished to obtain the complex acid repairing gel.
EXAMPLE 10 Mild Complex acid repair gel
The formula comprises the following components in parts by weight: 27 parts of lactobacillus fermentation extract, 20 parts of salicylic acid, 3 parts of octanoylsalicylic acid, 1 part of glycollic acid, 4 parts of mandelic acid, 2 parts of citric acid, 3 parts of lactic acid, 5 parts of tween 80 and 35 parts of water.
The lactobacillus fermentation extract is as follows: inoculating single colony of lactobacillus (Lactobacillus plantarum AS 1.555) into MRS culture medium, activating and culturing at 37deg.C for 24 hr to obtain seed solution, inoculating seed solution into MRS culture medium containing 0.02g/L threonine according to 4w/w% inoculum size, fermenting and culturing at 37deg.C for 36 hr, adding 0.02g/L serine into fermentation culture medium, and fermenting and culturing for 36 hr to obtain lactobacillus fermentation liquid. Centrifuging the lactobacillus fermentation liquid at 2000rpm for 10min, collecting supernatant, and respectively collecting first components with molecular weight of 5000-15000 Da (replacing 2.5 parts of Tween 80 after drying) and second components with molecular weight of 15000-30000 Da (used as lactobacillus fermentation extract in the formula after drying) by membrane separation method.
The preparation method of the composite acid repair gel comprises the following steps:
(1) Adding water into the second component to prepare 70w/w% extracellular polysaccharide solution to obtain phase A;
(2) Mixing salicylic acid and octanoyl salicylic acid to obtain phase B; slowly adding phase A into phase B, and mixing at 50+ -5deg.C (50 rpm,3 h);
(3) Uniformly mixing the glycolic acid, the citric acid, the mandelic acid, the lactic acid, the tween 80 and the first component, and adding the balance of water to obtain a phase C;
(4) And slowly dripping the mixture obtained in the step (2) into the C phase at 25 ℃, keeping stirring at 50rpm in the dripping process, and continuing stirring for 1h after the dripping is finished to obtain the complex acid repairing gel.
Experimental example: detection of inhibition of Propionibacterium acnes Activity
An agar plate diffusion method (punching method) was used. Formulation 10 7 cfu/mL Propionibacterium acnes suspension, 2mL of the bacterial suspension was uniformly spread on a medium plate, 6mm diameter holes were punched in the plate with a sterile punch, and samples of each example diluted 5-fold with water (stirred at 100rpm for 5min before use) were added to the holes to make a blank with sterile water. Culturing in an incubator for 72 hours, measuring the diameter of the inhibition zone, repeating the test for three times, and taking the average value. The inhibition growth rate refers to the growth rate of the inhibition zone diameter relative to the blank control. The results are shown in Table 1.
TABLE 1
The test results show that the diameter of the inhibition zone reaches a significant difference (P < 0.01) compared with the blank control in the samples of each treatment group. Comparing the results of examples 1 and 2, it is clear that the antibacterial effect of the lactobacillus plantarum fermented extract is better than that of polysaccharide extracted from traditional dairy products (P < 0.01). Comparing the results of example 4 and example 7, it is evident that the effect of selecting Lactobacillus plantarum fermented extract is better than Lactobacillus brevis (P < 0.01). Comparing the results of examples 3, 4 and 5, it is known that threonine and serine can be sequentially added in the culturing process to induce saccharomycetes to produce efficient antibacterial products (the diameters of antibacterial rings of example 3 and example 5 are obviously different from those of example 4, and P is less than 0.01). Compared with the embodiment 4, the first component can replace Tween 80 to be used as an emulsifier, and has a certain antibacterial effect.
In the whole, the invention combines different types of monoacids with lactobacillus fermentation products to play the compensation and synergy mechanisms among the components, and further combines the unique multi-layer microencapsulation preparation method, so that the components are released at a constant speed in the use process, and the product is mild, not stimulated and good in stability. The safety and the effectiveness of the product are clinically verified.
The application method of the product comprises the following steps:
the product is smeared on the face uniformly by using a brush, the face is left for about 2 minutes, the face is gently and circularly massaged by using a massage technique, and the fingers are smeared with a proper amount of purified water or normal saline, so that the compound acid skin refreshing liquid is kept in a wet state at any time, sensitive parts are avoided while the massage technique is assisted, the parts with heavier symptoms are massaged in a focus way, the massage is performed for about 15 minutes, and the gauze is smeared with clean water to terminate the reaction. The product can realize the uniform release of the functional components within 15min, avoid the quick release of the stimulus to local skin, and simultaneously, the use feeling is not affected due to reasonable release time.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (5)
1. The multi-layer microencapsulated complex acid repair gel based on the lactobacillus fermentation product EPS is characterized by comprising the following components in parts by weight: 20-27 parts of lactobacillus fermentation extract, 15-20 parts of salicylic acid, 1-3 parts of octanoyl salicylic acid, 1-2 parts of glycollic acid, 3-5 parts of mandelic acid, 1-2 parts of citric acid, 1-3 parts of lactic acid and an emulsifier;
the preparation method of the lactobacillus fermentation extract comprises the following steps: inoculating lactobacillus seed liquid to MRS culture medium containing 0.02-0.05 g/L threonine, fermenting and culturing for 24-36 h, adding 0.02-0.05 g/L serine into the fermentation culture medium, and continuing fermenting and culturing for 24-36 h to obtain lactobacillus fermentation liquid; centrifuging lactobacillus fermentation liquor, taking supernatant, collecting components with molecular weight between 15000 and 30000Da, and obtaining lactobacillus fermentation extract, drying for later use;
the lactobacillus is Lactobacillus plantarum or Lactobacillus brevis.
2. The multi-layered microencapsulated complex acid repair gel based on lactobacillus fermentation product EPS according to claim 1 wherein the lactobacillus fermentation extract comprises extracellular polysaccharide.
3. The multi-layer microencapsulated complex acid repair gel based on the lactic acid bacteria fermentation product EPS of claim 1, characterized in that the lactic acid bacteria fermentation liquid is centrifuged, the supernatant is taken, the first component with the molecular weight between 5000 and 15000Da and the second component with the molecular weight between 15000 and 30000Da are respectively collected and dried for standby; the second component is lactobacillus fermentation extract in the formula; the addition amount of the first component in the formula is 0-5 parts.
4. The method for preparing the multi-layer microencapsulated complex acid repair gel based on the lactobacillus fermentation product EPS according to claim 1, characterized by comprising the steps of:
(1) Adding aqueous solution into lactobacillus fermentation extract to obtain phase A;
(2) Mixing salicylic acid and octanoyl salicylic acid to obtain phase B; slowly adding the phase A into the phase B, and mixing at 45-55 ℃;
(3) Uniformly mixing other components in the formula, and adding water to obtain a phase C;
(4) And slowly dripping the mixture obtained in the step (2) into the phase C, keeping stirring in the dripping process, and continuing stirring after the dripping is finished to obtain the complex acid repairing gel.
5. Use of the multi-layered microencapsulated complex acid repair gel based on lactobacillus fermentation product EPS according to claim 1 for the preparation of skin care products.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2003259860A (en) * | 2002-03-06 | 2003-09-16 | Meishun Tei | Lactococcus lactis cbt-19, method for producing separated concentrate of antibacterial cultured liquid by using the same and cosmetic composition containing the same |
| KR20140054679A (en) * | 2012-10-29 | 2014-05-09 | 주식회사 태영건설 | Culture method of lactobacillus lactic acid bacteria and the volatile fatty acids production method based on the culture |
| CN110507598A (en) * | 2019-09-10 | 2019-11-29 | 河北一然生物科技有限公司 | Lactic acid bacteria is inhibiting the application in propionibacterium acnes and skin care item made of it |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003259860A (en) * | 2002-03-06 | 2003-09-16 | Meishun Tei | Lactococcus lactis cbt-19, method for producing separated concentrate of antibacterial cultured liquid by using the same and cosmetic composition containing the same |
| KR20140054679A (en) * | 2012-10-29 | 2014-05-09 | 주식회사 태영건설 | Culture method of lactobacillus lactic acid bacteria and the volatile fatty acids production method based on the culture |
| CN110507598A (en) * | 2019-09-10 | 2019-11-29 | 河北一然生物科技有限公司 | Lactic acid bacteria is inhibiting the application in propionibacterium acnes and skin care item made of it |
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Address after: No. 3701, 37th Floor, Building 2, No. 666, Middle Tianfu Avenue, Chengdu High-tech Zone, China (Sichuan) Pilot Free Trade Zone, Chengdu, Sichuan 610000 Patentee after: Chunyan Biotechnology Co.,Ltd. Country or region after: China Address before: 37th floor, building 2, No. 666, middle section of Tianfu Avenue, Chengdu hi tech Zone, China (Sichuan) pilot Free Trade Zone, Chengdu, Sichuan 610000 Patentee before: Chayan Biotechnology Co.,Ltd. Country or region before: China |