CN114847415A

CN114847415A - Functional traditional Chinese medicine bait for preventing influenza

Info

Publication number: CN114847415A
Application number: CN202110154117.5A
Authority: CN
Inventors: 张朔生; 李秀英; 史鸾霞; 张晓燕; 孟祥龙
Original assignee: Shanxi University of Chinese Mediciine
Current assignee: Shanxi University of Chinese Mediciine
Priority date: 2021-02-04
Filing date: 2021-02-04
Publication date: 2022-08-05

Abstract

The invention discloses a functional traditional Chinese medicine fish bait for preventing influenza. The traditional Chinese medicine fish bait comprises the following components in percentage by mass: 1 to 10 percent of traditional Chinese medicine compound extract, 35 to 45 percent of corn flour, 25 to 35 percent of wheat middling, 10 to 15 percent of fish meal, 3 to 5 percent of beer yeast, 3 to 6 percent of soybean oil, 1 to 2 percent of soybean lecithin, 1 percent of calcium carbonate, 0.45 to 0.55 percent of sodium alginate, 0.1 to 0.4 percent of choline chloride, 0.3 to 0.6 percent of mixed vitamin, 0.3 to 0.5 percent of mixed inorganic salt, 0.2 to 0.4 percent of taurine, 0.3 percent of thiobetaine and 0.3 percent of ferrous lactate; the traditional Chinese medicine compound extracting solution is prepared from the following components in parts by mass: 15 parts of honeysuckle; 10 parts of red peony root; and 3 parts of rhizoma dryopteris crassirhizomae. The functional traditional Chinese medicine fish bait for preventing influenza provided by the invention can obviously improve the stability and the utilization rate of the feed, can promote the health of fishes, improves the ingestion capability of the fishes, improves the immunity and the anti-stress capability of the fishes, and promotes the healthy growth of the fishes.

Description

Functional traditional Chinese medicine bait for preventing influenza

Technical Field

The invention relates to a functional traditional Chinese medicine fish bait for preventing influenza, and belongs to the technical field of feeds.

Background

China is the first aquaculture country in the world. In recent years, the marine fish breeding industry develops rapidly, and the feed demand is greatly increased. The various properties of the feed directly influence the food intake of fishes, the amount of residual feed, the growth speed and the utilization efficiency of the feed, and can directly influence the economic benefit and the ecological benefit of cultivation. The prior commonly used fish feed has poor stability in water and high settling speed, which not only causes the waste of feed nutrition, but also causes the serious pollution of water quality.

Influenza is a disease susceptible to fish, seriously affects the health of fish and further affects the economic benefit of fish culture. Fish are sensitive to changes in water temperature, and a sharp increase or decrease in water temperature stimulates nerve endings of the skin, causing a disorder in the activity of internal organs, thereby causing colds to occur. The main manifestations are that the skin of the fish loses the original luster, the color becomes pale, the body surface mucus increases, the color of the fish body becomes dark, the fish is not easy to eat, the fish does not swim actively, even floats on the water surface or lies at the water bottom, when the illness is serious, the fish often loses the activity, and the fish dies in a few hours or days. In this case, the fish should be fed with a drug for the prevention and treatment of influenza. However, no solution is currently available for the prevention of this disease, and there is a need to provide a functional fish bait for the prevention of influenza.

Disclosure of Invention

The invention aims to provide a functional traditional Chinese medicine fish bait for preventing influenza, which has strong water resistance and floatability, can prevent influenza, reduce the risk of fish diseases and further increase the economic benefit of fish culture.

The functional traditional Chinese medicine fish bait for preventing influenza provided by the invention comprises the following components in percentage by mass:

1 to 10 percent of traditional Chinese medicine compound extract, 35 to 45 percent of corn flour, 25 to 35 percent of wheat middling, 10 to 15 percent of fish meal, 3 to 5 percent of beer yeast, 3 to 6 percent of soybean oil, 1 to 2 percent of soybean lecithin, 1 percent of calcium carbonate, 0.45 to 0.55 percent of sodium alginate, 0.1 to 0.4 percent of choline chloride, 0.3 to 0.6 percent of mixed vitamin, 0.3 to 0.5 percent of mixed inorganic salt, 0.2 to 0.4 percent of taurine, 0.3 percent of thiobetaine and 0.3 percent of ferrous lactate;

the traditional Chinese medicine compound extracting solution is prepared from the following components in parts by mass:

15 parts of honeysuckle; 10 parts of red peony root; and 3 parts of rhizoma dryopteris crassirhizomae.

Specifically, the traditional Chinese medicine compound extracting solution is prepared according to the following steps:

adding 10-20 times of water into the honeysuckle for distillation to obtain distillate;

mixing the red peony root and the rhizoma dryopteris crassirhizomae, adding water for soaking, then decocting for 1-2 times, and combining decoction liquids;

according to the following steps: 13, mixing the distillate and the decoction, and concentrating under reduced pressure to obtain the compound traditional Chinese medicine extracting solution.

Specifically, in the decocting process, the amount of water is 5-7 ml/g of a mixed material, preferably 6ml/g, and the mixed material is a mixture of the red peony root and the male fern rhizome.

Specifically, the soaking time is 1.5-2.5 hours, and preferably 2 hours.

Specifically, the time for each decoction is 1-2 hours, preferably 1.5 hours.

Specifically, the relative density of the traditional Chinese medicine compound extracting solution is 1.0 g/ml.

Specifically, the mixed vitamins include vitamin A acetate (vitamin A), vitamin D ₃ Alpha-tocopheryl acetate (vitamin E), menadione sodium bisulfite (vitamin K) ₃ ) Thiamine nitrate (vitamin B) ₁ ) Riboflavin (vitamin B) ₂ ) Pyridoxine hydrochloride (vitamin B) ₆ ) Cyanocobalamin (vitamin B) ₁₂ ) D-biotin, folic acid, niacinamide, D-calcium pantothenate, L-ascorbic acid (vitamin C), inositol, and ethoxyquinoline;

the mixed inorganic salt comprises potassium chloride, magnesium sulfate, ferric sulfate, copper sulfate, zinc carbonate, zinc sulfate, cobalt carbonate, manganese sulfate, potassium iodate, calcium iodate, salt, zinc phosphate, calcium phosphate and sodium selenite.

The functional traditional Chinese medicine fish bait for preventing influenza preferably comprises the following components in percentage by weight:

2% of traditional Chinese medicine compound extract, 40% of corn flour, 30% of wheat middling, 13.7% of fish meal, 4% of beer yeast, 5% of soybean oil, 1.5% of soybean lecithin, 1% of calcium carbonate, 0.5% of sodium alginate, 0.5% of choline chloride, 0.5% of mixed vitamin, 0.4% of mixed inorganic salt, 0.3% of taurine, 0.3% of sulfobetaine and 0.3% of ferrous lactate.

The invention also provides a preparation method of the functional traditional Chinese medicine fish bait, which comprises the following steps:

weighing the components of the functional traditional Chinese medicine fish bait according to the mass percentage, uniformly mixing, and adding the traditional Chinese medicine compound extract and water under the stirring condition to obtain a material to be puffed; puffing the material to be puffed, such as puffing at 180 deg.C.

The functional traditional Chinese medicine fish bait for preventing influenza provided by the invention can obviously improve the stability and the utilization rate of the feed, can promote the health of fishes, improves the ingestion capability of the fishes, improves the immunity and the anti-stress capability of the fishes, and promotes the healthy growth of the fishes.

Drawings

FIG. 1 shows the effect of water addition on the decoction process.

FIG. 2 is a graph showing the effect of the decoction time on the decoction process.

FIG. 3 is a graph showing the effect of the number of times of decoction on the decoction process.

FIG. 4 is a graph showing the effect of soaking time on the decoction process.

FIG. 5 is a graph showing the effect of the amount of water added and the amount of decoction on the paeoniflorin content (FIG. 5(a) is a contour diagram, and FIG. 5(b) is a three-dimensional response curve).

FIG. 6 is a graph showing the effect of the decoction time and the number of times of decoction on the paeoniflorin content (FIG. 6(a) is a contour diagram, and FIG. 6(b) is a three-dimensional response curve diagram).

FIG. 7 is a graph showing the influence of the amount of water added and the number of times of decoction on the paeoniflorin content (FIG. 7(a) is a contour diagram, and FIG. 7(b) is a three-dimensional response curve).

Fig. 8 is a sign index of fish fed with the functional traditional Chinese medicine bait for preventing influenza (fig. 8(a) is a weight graph of an experimental fish, fig. 8(b) is a fatness graph of the experimental fish, and fig. 8(c) is an efficiency graph of the experimental feed, wherein # represents P <0.05 compared with a normal group, and # represents P <0.05 compared with a temperature-variable control group).

Fig. 9 is a biochemical index of fish raised by functional traditional Chinese medicine bait feed for preventing influenza (fig. 9(a) is an experimental fish serum total protein map, fig. 9(b) is an experimental fish serum globulin map, fig. 9(c) is an experimental fish serum ALT map, fig. 9(d) is an experimental fish serum AKP map, fig. 9(e) is an experimental fish serum AST map, fig. 9(f) is a liver pancreas superoxide dismutase SOD map, fig. 9(g) is a liver pancreas malonaldehyde map, and fig. 9(h) is a liver pancreas MDA map, wherein P is <0.05 compared with a variable temperature control group, # CAT is P <0.01 compared with a variable temperature control group, # is P <0.05 compared with a normal group, and # is P # is P <0.01 compared with a normal group).

Detailed Description

The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.

Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.

Chromatographic conditions for detecting paeoniflorin content:

the content of paeoniflorin is determined by high performance liquid chromatography, octadecylsilane chemically bonded silica is used as filler, a chromatographic column is a Tnature-C18 column (250mm multiplied by 4.6mm, 5 μm), the volume flow is 1mL/min, the column temperature is 30 ℃, the mobile phase is acetonitrile-0.1% phosphoric acid water (14: 86), and the detection wavelength is 230 nm.

Preparation of control solutions:

precisely weighing appropriate amount of penoniflorin as control, adding water to obtain solution each containing 1.5mg/mL, performing ultrasonic treatment for 20min, and filtering with 0.22 μm microporous filter membrane to obtain control solution.

Preparation of a test solution:

decocting radix Paeoniae Rubra and rhizoma Dryopteris Crassirhizomatis in water, evaporating to dry, collecting appropriate amount of extract, adding water to constant volume of 5mL, and performing ultrasonic treatment for 20 min.

Distilling flos Lonicerae with water, and collecting distillate.

Mixing the two solutions, and filtering with 0.22 μm microporous membrane to obtain test solution.

And (3) sample determination:

precisely sucking 10 μ L of each of the reference solution and the sample solution, and injecting into a liquid chromatograph for content determination.

Example 1 study of extraction Process of Chinese herbal Compound for preventing influenza

The formula of the traditional Chinese medicine compound extract for preventing influenza comprises the following components:

honeysuckle flower 15g, red peony root 10g, male fern rhizome 3 g.

Adding water into flos Lonicerae, and distilling to obtain distillate;

mixing radix Paeoniae Rubra and rhizoma Dryopteris Crassirhizomatis, soaking in water, decocting, and mixing decoctions; mixing the distillate and the decoction, and concentrating under reduced pressure to obtain compound Chinese medicinal extractive solution.

And (3) carrying out single factor investigation and response surface design analysis on the water adding amount, the decoction times and the decoction time in the extraction process by taking paeoniflorin as an index.

1. Research on extraction process of Chinese herbal compound

1. Single factor experiment

1.1 influence of Water addition on the decocting Process

Taking 10g of red peony root and 3g of rhizoma dryopteris crassirhizomae, putting 5 parts of the total into a round-bottom flask, respectively adding 4, 6, 8, 10 and 12 times of water, soaking for 0.5h, decocting for 2 times, each time for 1h, combining decoction, centrifuging, taking supernatant, evaporating to dryness, and adding water to a constant volume of 5 mL. The results are shown in FIG. 1. As can be seen, the paeoniflorin content was the highest at 6 times the water volume.

1.2 Effect of decoction time on the decoction Process

Taking 10g of red paeony root and 3g of rhizoma dryopteris crassirhizomae, putting 5 parts of the total into a round-bottom flask, respectively adding 8 times of water, soaking for 0.5h, decocting for 2 times, decocting for 1h, 1.5h, 2h, 2.5h and 3h each time, respectively, combining decoction, centrifuging, taking supernate, drying by distillation, and adding water to a constant volume of 5 mL. The results are shown in FIG. 2. As can be seen, the paeoniflorin content is highest under the decoction time of 1.5 h.

1.3 Effect of the number of decocts on the decoct Process

Taking 10g of red peony root and 3g of rhizoma dryopteris crassirhizomae, putting 5 parts of the total into a round-bottom flask, adding 8 times of water, soaking for 0.5h, decocting for 1, 2, 3, 4 and 5 times respectively, each time for 1h, combining decoction, centrifuging, taking supernate, evaporating to dryness, and adding water to a constant volume of 5 mL. The results are shown in FIG. 3. It can be seen that paeoniflorin content is highest when the number of times of decoction is 2.

1.4 influence of soaking time on decocting Process

Taking 10g of red peony root and 3g of rhizoma dryopteris crassirhizomae, putting 5 parts of the raw materials into a round-bottom flask, adding 8 times of water, decocting for 0.5, 1, 1.5, 2 and 2.5 hours respectively, decocting for 2 times, decocting for 1 hour each time, combining decoction, centrifuging, taking supernate, evaporating to dryness, and adding water to a constant volume of 5 mL. The results are shown in FIG. 4. As can be seen, the paeoniflorin content is highest when the soaking time is 2 h.

2. Response surface experiment

2.1 design scheme

According to the single-factor test result, an optimization experiment is carried out by taking the soaking time as 2h, the decocting time (A), the water adding amount (B) and the decocting times (C) as investigation factors and the paeoniflorin content (Y) as a response value, and a 3-factor three-level experiment design scheme is designed, wherein the design scheme is shown in table 1.

TABLE 1 response surface analysis factors and level design of extraction process of traditional Chinese medicine components for preventing influenza

2.2 model establishment and significance test result analysis

3-factor 3-level 17 sets of tests are designed by using Design-expert.V8.0.6 software according to the center combination Design principle of Box-Behnken Design (BBD), and the test scheme and the results are shown in Table 2.

Table 2 design and results of response surface experimental protocol

ANOVA analysis is carried out on the decoction process of the Chinese herbal compound for preventing influenza at different levels by adopting a Design Expert program, contour diagrams and three-dimensional response surface diagrams are drawn, and the results are shown in figures 5, 6 and 7. After regression fitting, the regression equation of each factor and the response value is as follows:

content of paeoniflorin is 2.16 + 0.01A + 0.25 AC-0.12B-0.38C-0.18 AB-0.18 BC-0.37A ² ﹣0.30B ² ﹣0.40C ²

Regression parameters of the effector surface were analyzed by ANOVA, and the simulations were subjected to analysis of variance and significance tests, with the results shown in table 3.

TABLE 3 analysis of variance results

As can be seen from Table 3, in the significance test of the model, F is 4.92, P is 0.0237 is less than 0.05, which indicates that the model has significance, thereby demonstrating that the model can be used for the treatment of paeoniflorinDecocting for prediction and analysis; and the model determines the coefficient R ² ＝0.8635，R ² _Adj 0.6879, Coefficient of Variation (CV) 17.27%, indicating a good model fit; f mismatch is 5.79, P is more than 0.05, model mismatch is not significant, model selection is more appropriate, and A, A2 and B are important model items. F loss is 5.79, which indicates that the model can be used for guiding the extraction process research of the traditional Chinese medicine prescription for preventing influenza.

The response surface method is utilized to carry out reasonable optimization, and the optimal process for obtaining the comprehensive influence of each influence factor is as follows: adding water 5.87 times, decocting for 1.50 hr for 1.54 times, and radix Paeoniae glycoside content is 2.2537 mg/g. Considering the convenience of actual operation, 6 times of water is added, the soaking time is 2h, and the decoction is carried out for 2 times, and each time is 1.5 h.

3. Verification experiment

Taking the same batch of medicinal materials, taking 3 parts in parallel, decocting according to the optimal decoction process of the compound prescription for preventing influenza, and inspecting the content of paeoniflorin in the liquid medicine, wherein the results are shown in table 4. The model is similar to the model predicted value (2.2537mg/ml), which shows that the established process model has better predictability, the optimized processing process is reasonable, and the method has guiding significance for actual production.

Table 4 process verification experimental results

Example 2 preparation of functional traditional Chinese medicine fish bait for preventing influenza

1. Preparation of traditional Chinese medicine compound liquid medicine with function of preventing influenza

Extracting the traditional Chinese medicine liquid of red paeony root and male fern rhizome according to the optimal extraction process, wherein the traditional Chinese medicine liquid is prepared from 15 parts of honeysuckle flower, 10 parts of red paeony root and 3 parts of male fern rhizome according to the proportion of the traditional Chinese medicine compound extract (13: 15, mixing the traditional Chinese medicine liquid with honeysuckle liquid obtained by 20 times of medicinal materials through steam distillation, and concentrating under reduced pressure until the relative density is 1.0 to obtain the functional traditional Chinese medicine active ingredient for preventing influenza.

2. Dosage and adding mode of Chinese medicinal components

The dosage of the traditional Chinese medicine components is as follows: the traditional Chinese medicine effective extract is added according to the mass ratio of 2 percent of the whole fish bait. Therefore, 1kg of fish bait is prepared, and 20mL of the traditional Chinese medicine effective extract liquid with the concentration of 1g/mL is added.

The adding mode is as follows: adding the Chinese medicinal effective extract liquid into water in the form of adhesive.

3. Preparation and evaluation of anti-influenza puffing gas-producing floating fish bait

The basic fish feed formula comprises the following components: 40% of corn flour, 30% of wheat middling, 13.7% of fish meal, 4% of beer yeast, 5% of soybean oil, 1.5% of soybean lecithin, 1% of calcium carbonate, 0.5% of sodium alginate, 0.5% of choline chloride, 0.5% of vitamin mixture, 0.4% of inorganic salt mixture, 0.3% of taurine, 0.3% of thiobetaine and 0.3% of ferrous lactate;

wherein the mixed vitamins include vitamin A acetate (vitamin A) and vitamin D ₃ Alpha-tocopheryl acetate (vitamin E), menadione sodium bisulfite (vitamin K) ₃ ) Thiamine nitrate (vitamin B) ₁ ) Riboflavin (vitamin B) ₂ ) Pyridoxine hydrochloride (vitamin B) ₆ ) Cyanocobalamin (vitamin B) ₁₂ ) D-biotin, folic acid, niacinamide, D-calcium pantothenate, L-ascorbic acid (vitamin C), inositol, ethoxyquinoline; the mixed inorganic salt comprises potassium chloride, magnesium sulfate, ferric sulfate, copper sulfate, zinc carbonate, zinc sulfate, cobalt carbonate, manganese sulfate, potassium iodate, calcium iodate, salt, zinc phosphate, calcium phosphate, and sodium selenite.

Weighing the components of the basic fish bait according to the mass parts, uniformly mixing, adding the flu-preventing traditional Chinese medicine liquid and a proper amount of water under the stirring condition, and preparing the material to be puffed. Then adding the mixture into a feed bulking machine for bulking and granulating at 180 ℃.

The floating performance and the dissolving performance of the fish bait for preventing influenza are measured by referring to an appendix release degree measuring method (paddle method) in the fourth part of Chinese pharmacopoeia of 2015 edition. Researches show that the prepared puffed bait material for preventing influenza has the floating time of 12 hours and the dissolving time of 24 hours.

Example 3 pharmacodynamic experiment study of functional traditional Chinese medicine fish feed for preventing influenza

1. Experimental methods

Randomly dividing 60 crucian juvenile fishes into a normal group, a variable temperature control group and a variable temperature experimental group, wherein 20 crucian juvenile fishes in each group are placed in a fish tank with the constant temperature of 25 ℃, the variable temperature control group and the variable temperature experimental group are placed in a variable temperature fish tank, and the water temperature is controlled to be 5-8 ℃ in the morning and evening and 20 ℃ in the noon. The normal group and the variable-temperature control group are fed with common puffed fish bait, and the variable-temperature experimental group is fed with functional traditional Chinese medicine fish bait for preventing influenza in an equivalent amount for 60 days. The number of the experimental fishes, whether the fishes die or not, the weight, the body length and the feeding days are recorded in the experiment, and the growth performance index is calculated.

Weight gain rate ═ (terminal weight-initial weight)/initial weight 100%;

specific growth rate [ Ln (average end body weight) -Ln (average initial body weight) ]/day of food intake 100%;

body weight/body length ³ *100％；

Feed efficiency is fish body weight gain (wet weight)/total dry matter intake;

survival rate is terminal fish number/initial fish number 100%;

after the rearing was finished, the experimental fish was weighed. Taking blood, storing serum at-80 ℃ after quenching in liquid nitrogen, measuring serum total protein and globulin by a biochemical analyzer, carrying out ALT, AKP and AST on the serum according to the steps in the kit specification, dissecting and weighing the heart, liver, kidney and the like of the experimental fish, and calculating the visceral coefficient. Visceral index is organ mass/body weight 100%. The SOD value of liver and pancreas, MDA and CAT are operated according to the steps in the kit specification, and the differences of the common puffed bait and the puffed traditional Chinese medicine fish bait for preventing flu on the anti-flu effect of fish are compared.

2. Index of drug effect

2.1 indicators of physical signs

As shown in table 5 and fig. 8(a), the body weight of the variable temperature experimental group was significantly increased compared to the variable temperature control group, and was not significantly different from the normal group, while ensuring that the initial body weight of the fish in each experimental group was not different. Compared with the temperature-changing control group, the body length of the fish in the temperature-changing experimental group is obviously increased, and has no obvious difference with that in the normal group. Compared with the temperature-changing control group, the fullness of the fish in the temperature-changing experimental group is significantly improved, and has no significant difference with the normal group (fig. 8 b). Compared with the temperature-variable control group, the feed efficiency of the temperature-variable experimental group was significantly increased, and was not significantly different from that of the normal group (fig. 8 c). Compared with a blank control group, the temperature-changing experimental group has a faster growth rate and a significant difference, and the difference is not large compared with a normal group.

According to the sign index results, the functional traditional Chinese medicine fish bait for preventing influenza can better maintain normal growth of fish under the low-temperature condition, and has small pollution to water quality.

2.2 Biochemical index

As shown in fig. 9 and table 5, the total serum protein of the temperature-shifted experimental group was significantly increased compared to the temperature-shifted control group, and was close to that of the normal group (fig. 9 (a)). Compared with the temperature-shifted control group, the serum globulin content of the temperature-shifted experimental group was significantly increased (fig. 9(b)), and was not significantly different from that of the normal group. Compared with the temperature-variable control group, the contents of ALT and AST in the fish serum of the temperature-variable experimental group are both increased remarkably, and have no remarkable difference with the normal group (fig. 9(c) and 9 (e)). Compared with the temperature-changing control group, the liver and kidney visceral coefficients of the experimental group are obviously increased, and have no obvious difference with the normal group. Compared with the temperature-variable control group, the SOD and CAT contents of the temperature-variable experimental group have significant difference (FIGS. 9(f) and 9(h)), and have no significant difference from the normal group.

The biochemical index result shows that the functional traditional Chinese medicine bait for preventing influenza obviously improves the biochemical index of the fish under the low-temperature condition, thereby having better effect of preventing influenza and promoting the growth of the fish.

In conclusion, the functional traditional Chinese medicine fish bait for preventing influenza can promote the health of fishes, promote the normal growth of the fishes, improve the disease resistance of the fishes and reduce the pollution to water quality.

TABLE 5 drug effect of functional Chinese medicinal fish bait for preventing influenza

Comparing with the normal group at variable temperature (p <0.05) # (p <0.01), comparing with the normal group (p <0.05) # # (p <0.01)

Example 4 evaluation of safety of functional traditional Chinese medicine fish feed for preventing influenza

The grading criteria for acute toxicity of fish are shown in table 6 below:

TABLE 6 grading Standard of acute toxicity of Fish

Through experimental observation, when the traditional Chinese medicine compound extract is fed by 50g/L, no death condition occurs in an experimental group, and the movement state of the fish is normal, which shows that the LD of the traditional Chinese medicine compound extract is ₅₀ >10 ⁴ mg/L, as can be seen from Table 6, the acute toxicity grade of the compound traditional Chinese medicine component is slightly toxic (nontoxic), and the toxicity of the compound traditional Chinese medicine extract with the content of 10% is tested, and has no substantial difference with the toxicity, so that the functional traditional Chinese medicine fish bait for preventing influenza with the compound traditional Chinese medicine component content of 1% -10% is safer. The difference between the experimental group and the normal group in the 60-day efficacy experiment is combined, and the safety of the traditional Chinese medicine fish bait is also proved.

Claims

1. A functional traditional Chinese medicine fish bait for preventing influenza comprises the following components in percentage by mass:

2. The functional traditional Chinese medicine fish bait as claimed in claim 1, which is characterized in that: the traditional Chinese medicine compound extracting solution is prepared according to the following steps:

adding water with the amount of 10-20 times of the medicinal materials into the honeysuckle, and distilling to obtain distillate;

and mixing the distillate and the decoction, and concentrating under reduced pressure to obtain the compound traditional Chinese medicine extracting solution.

3. The functional traditional Chinese medicine fish bait as claimed in claim 2, which is characterized in that: in the decocting process, the amount of the water is 5-7 ml/g of a mixed material, and the mixed material is a mixture of the red peony root and the male fern rhizome.

4. The functional traditional Chinese medicine fish bait as claimed in claim 2 or 3, which is characterized in that: the soaking time is 1.5-2.5 h.

5. The functional traditional Chinese medicine fish bait according to any one of claims 2 to 4, which is characterized in that: the time for decocting each time is 1-2 hours.

6. The functional traditional Chinese medicine fish bait as claimed in claim 5, which is characterized in that: the amount of the water is 6ml/g of the mixed material;

the soaking time is 2 hours;

the number of times of decoction is 2;

the time for decocting each time is 1.5 h;

the relative density of the traditional Chinese medicine compound extracting solution is 1.0 g/ml.

7. The functional traditional Chinese medicine fish bait according to any one of claims 1 to 6, which is characterized in that: the mixed vitamins include vitamin A acetate and vitamin D ₃ Alpha-tocopheryl acetate, menadione sodium bisulfite, thiamine nitrate, riboflavin, pyridoxine hydrochloride, cyanocobalamine, D-biotin, folic acid, nicotinamide, D-calcium pantothenate, L-ascorbic acid, myo-inositolAlcohols and ethoxyquinolines;

8. A method for preparing the functional Chinese medicine fish bait as claimed in any one of claims 1 to 7, which comprises the following steps:

weighing the components of the functional traditional Chinese medicine fish bait according to the mass percentage, uniformly mixing, and adding the traditional Chinese medicine compound extract and water under the stirring condition to obtain a material to be puffed; and (3) puffing the material to be puffed to obtain the puffed food.

9. Use of the functional Chinese medicinal fish bait according to any one of claims 1 to 7 for preventing fish flu.