CN114813590A

CN114813590A - Sample detection method, sample analyzer and control device thereof

Info

Publication number: CN114813590A
Application number: CN202210757478.3A
Authority: CN
Inventors: 肖华; 张志国; 胡彦勇
Original assignee: Shenzhen Dymind Biotechnology Co Ltd
Current assignee: Shenzhen Dymind Biotechnology Co Ltd
Priority date: 2022-06-30
Filing date: 2022-06-30
Publication date: 2022-07-29

Abstract

The application discloses sample detection method, sample analyzer and control device thereof, the sample detection method comprises: the light source module emits a detection light beam to the sample; the sensor module receives a transmitted beam and/or a reflected beam transmitted and/or reflected by the detection beam through the sample; the processing module determines whether the interference degree of the interferent in the sample reaches a preset degree threshold value based on the transmitted light beam and/or the reflected light beam; and when the interference degree does not reach the preset degree threshold value, the processing module controls the optical detection module to detect the sample based on an optical detection method. Based on the mode, the sample detection efficiency can be effectively improved.

Description

Sample detection method, sample analyzer and control device thereof

Technical Field

The present application relates to the field of detection technologies, and in particular, to a sample detection method, a sample analyzer, and a control device thereof.

Background

In the prior art, samples such as plasma, serum, urine, etc. are usually collected from a patient, and the concentration and activity of a specific substance in the samples are detected to obtain corresponding detection data. Taking the plasma sample as an example, the detection method involved in the detection generally includes an optical detection method and a magnetic bead method, wherein the optical detection method is low in cost, so the sample is generally detected by the optical detection method.

The prior art has the defect that if the collected sample contains interferents corresponding to symptoms of hemolysis, chyle, jaundice and the like, the color of the sample is easy to change. For example, a plasma sample containing an interferent against chylomicron symptoms may appear milky white, a plasma sample containing an interferent against jaundice symptoms may appear dark yellow or yellowish green, a plasma sample containing a hemolytic agent may appear red, and normal plasma may appear yellowish or even almost transparent, and such a change in the color of the sample may easily cause difficulty in obtaining a correct analysis result when the sample is detected by an optical detection method. If the sample detection by the optical detection method fails, the sample provider needs to collect the sample again, and another detection method (such as a paramagnetic particle method) is used to detect the newly collected sample, so that the whole sample collection process consumes too much time, and the detection efficiency of the conventional sample detection method is low.

Disclosure of Invention

The technical problem that this application mainly solved is how to improve sample detection efficiency.

In order to solve the above technical problem, the first technical solution adopted by the present application is: a sample identification method of a sample detection method is applied to a sample analyzer of a sample identification device, wherein the sample analyzer of the sample identification device comprises a light source module, a sensor module, a processing module and an optical detection module; sample identification method the sample detection method comprises: the light source module emits a detection light beam to the sample; the sensor module receives a transmitted beam and/or a reflected beam transmitted and/or reflected by the detection beam through the sample; the processing module determines whether the interference degree of the interferent in the sample reaches a preset degree threshold value based on the transmitted light beam and/or the reflected light beam; and when the interference degree does not reach the preset degree threshold value, the processing module controls the optical detection module to detect the sample based on an optical detection method.

The sample analyzer also comprises a magnetic bead detection module; the sample detection method further comprises: and when the interference degree reaches a preset degree threshold value, the processing module controls the magnetic bead detection module to detect the sample based on a magnetic bead method.

Wherein, the sensor module receives the transmission light beam and/or the reflection light beam that the probe beam transmits through the sample, includes: the sensor module receives a transmitted light beam and/or a reflected light beam transmitted by the detection light beam through the sample, and acquires the corresponding light intensity of the red light beam, the light intensity of the green light beam and the light intensity of the blue light beam from the transmitted light beam and/or the reflected light beam; the processing module determines whether the interference degree of the interferent in the sample reaches a preset degree threshold based on the transmitted light beam and/or the reflected light beam, and comprises: the processing module determines whether the interference degree of the interferent in the sample reaches a preset degree threshold value based on the light intensity of the red light beam, the light intensity of the green light beam and the light intensity of the blue light beam.

Wherein, processing module determines whether the interference degree of the interferent in the sample reaches the preset degree threshold value based on the light intensity of the red light beam, the light intensity of the green light beam and the light intensity of the blue light beam, and includes: the processing module determines the color of the sample based on the light intensity of the red light beam, the light intensity of the green light beam and the light intensity of the blue light beam; the processing module determines that the interference degree of the interference object in the sample reaches a preset degree threshold value when the color of the sample reaches the preset threshold value, and determines that the interference degree of the interference object in the sample does not reach the preset degree threshold value when the color of the sample does not reach the preset threshold value.

Wherein the sensor module comprises a color sensor; the sensor module receives the transmitted light beam and/or the reflected light beam transmitted by the sample from the detection light beam, and acquires the corresponding light intensity of the red light beam, the green light beam and the blue light beam from the transmitted light beam and/or the reflected light beam, and the sensor module comprises: the sensor module receives a transmitted light beam and/or a reflected light beam transmitted by the sample from the detection light beam, and acquires the light intensity of the red light beam, the light intensity of the green light beam and the light intensity of the blue light beam from the transmitted light beam and/or the reflected light beam based on the color sensor.

Wherein the color sensor comprises a red filter, a green filter, a blue filter and a light intensity sensor; the sensor module acquires the light intensity of the corresponding red light beam, the light intensity of the corresponding green light beam and the light intensity of the corresponding blue light beam from the transmitted light beam and/or the reflected light beam based on the color sensor, and comprises the following steps: the sensor module filters out red light beams in the transmitted light beams and/or the reflected light beams through a red filter, and obtains the light intensity of the red light beams through a light intensity sensor; the sensor module filters out green light beams in the transmitted light beams and/or the reflected light beams through a green filter, and obtains the light intensity of the green light beams through a light intensity sensor; the sensor module filters out blue light beams in the transmitted light beams and/or the reflected light beams through a blue filter, and obtains the light intensity of the blue light beams through a light intensity sensor.

Wherein, processing module when the interference degree does not reach and predetermines the degree threshold value, control optical detection module detects the sample based on optical detection method, include: when the interference degree does not reach the preset degree threshold value, the processing module re-executes the step that the sensor module receives the transmitted light beam transmitted by the sample and/or the reflected light beam reflected by the sample, and the processing module determines whether the interference degree of the interferent in the sample reaches the preset degree threshold value or not based on the transmitted light beam and/or the reflected light beam; and when the frequency that the interference degree continuously does not reach the preset degree threshold value reaches the preset frequency threshold value, the processing module controls the optical detection module to detect the sample based on the optical detection method.

The sample analyzer also comprises a magnetic bead detection module; the sample detection method further comprises: and when the frequency that the interference degree does not reach the preset degree threshold value continuously does not reach the preset frequency threshold value, the processing module controls the magnetic bead detection module to detect the sample based on a magnetic bead method.

Wherein, the sensor module receives the transmission light beam and/or the reflection light beam that the probe beam transmits through the sample, includes: the sensor module receives a transmitted beam transmitted by the detection beam through the sample and a reflected beam reflected by the detection beam; the processing module determines whether the interference degree of the interferent in the sample reaches a preset degree threshold value based on the transmitted light beam and/or the reflected light beam, and comprises the following steps: the processing module determines whether the interference degree of the interference object in the sample reaches a preset degree threshold value or not based on the transmitted light beam, and determines whether the interference degree of the interference object in the sample reaches the preset degree threshold value or not based on the reflected light beam; if the processing module determines that the interference degree of the interferent in the sample does not reach the preset degree threshold value based on the transmitted light beam and determines that the interference degree of the interferent in the sample does not reach the preset degree threshold value based on the reflected light beam, the processing module judges that the interference degree does not reach the preset degree threshold value.

After the processing module determines whether the interference degree of the interfering object in the sample reaches the preset degree threshold value based on the transmitted light beam and determines whether the interference degree of the interfering object in the sample reaches the preset degree threshold value based on the reflected light beam, the sample detection method further includes: if the processing module determines that the interference degree of the interference object in the sample does not reach the preset degree threshold value based on the transmitted light beam and determines that the interference degree of the interference object in the sample reaches the preset degree threshold value based on the reflected light beam, or if the processing module determines that the interference degree of the interference object in the sample reaches the preset degree threshold value based on the transmitted light beam and determines that the interference degree of the interference object in the sample does not reach the preset degree threshold value based on the reflected light beam, re-executing the step of determining whether the interference degree of the interference object in the sample reaches the preset degree threshold value based on the transmitted light beam and determining whether the interference degree of the interference object in the sample reaches the preset degree threshold value based on the reflected light beam; if the processing module determines that the interference degree of the interference object in the sample does not reach the preset degree threshold value based on the transmitted light beam, and the processing module determines that the interference degree of the interference object in the sample reaches the preset degree threshold value based on the reflected light beam, or if the processing module determines that the interference degree of the interference object in the sample reaches the preset degree threshold value based on the transmitted light beam, and the processing module determines that the interference degree of the interference object in the sample does not reach the preset degree threshold value based on the reflected light beam, the processing module judges that the interference degree reaches the preset degree threshold value.

After the processing module determines whether the interference degree of the interfering object in the sample reaches the preset degree threshold value based on the transmitted light beam and determines whether the interference degree of the interfering object in the sample reaches the preset degree threshold value based on the reflected light beam, the sample detection method further includes: if the processing module determines that the interference degree of the interference object in the sample reaches the preset degree threshold value based on the transmitted light beam and determines that the interference degree of the interference object in the sample reaches the preset degree threshold value based on the reflected light beam, the processing module judges that the interference degree reaches the preset degree threshold value.

In order to solve the above technical problem, the second technical solution adopted by the present application is: a sample analyzer comprises a light source module, a sensor module, a processing module and an optical detection module; the light source module is used for emitting a detection light beam to the sample; the sensor module is used for receiving a transmitted beam transmitted by the detection beam through the sample and/or a reflected beam reflected by the detection beam; the processing module is used for determining whether the interference degree of the interferent in the sample reaches a preset degree threshold value or not based on the transmitted light beam and/or the reflected light beam; the processing module is further used for controlling the optical detection module to detect the sample based on the optical detection method when the interference degree does not reach the preset degree threshold value.

In order to solve the above technical problem, a third technical solution adopted by the present application is: a control device of a sample analyzer comprises a light source module, a sensor module, a processing module and an optical detection module; the light source module is used for emitting a detection light beam to the sample; the sensor module is used for receiving a transmitted beam transmitted by the detection beam through the sample and/or a reflected beam reflected by the detection beam; the processing module is used for determining whether the interference degree of the interferent in the sample reaches a preset degree threshold value or not based on the transmitted light beam and/or the reflected light beam; the processing module is further used for controlling the optical detection module to detect the sample based on the optical detection method when the interference degree does not reach the preset degree threshold value.

The beneficial effect of this application lies in: different from the prior art, the technical scheme of this application is through launching the detecting beam to the sample to whether the interference degree of high-end interferent reaches preset degree threshold value in the sample is confirmed based on corresponding transmission light beam and/or reflected light beam, and only adopt the optical detection method to detect the sample when the interference degree does not reach preset degree threshold value, the condition that fails when adopting the optical detection method to detect the sample, and need the secondary to gather the sample in order to carry out secondary sample detection takes place, has improved sample detection efficiency.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.

FIG. 1 is a schematic block diagram of one embodiment of a sample analyzer of the present application;

FIG. 2 is a schematic flow chart diagram of one embodiment of a sample detection method of the present application;

FIG. 3 is a schematic structural diagram of one embodiment of a light source module and a sensor module;

FIG. 4 is a schematic structural diagram of another embodiment of a light source module and a sensor module;

FIG. 5 is a schematic block diagram of another embodiment of a sample analyzer of the present application;

FIG. 6 is a schematic flow chart of another embodiment of a sample detection method of the present application;

FIG. 7 is a schematic block diagram of a further embodiment of a sample analyzer according to the present application;

FIG. 8 is a schematic structural view of an embodiment of a control device of the sample analyzer of the present application;

fig. 9 is a schematic structural view of a further embodiment of the sample analyzer of the present application.

Detailed Description

The present application will be described in further detail with reference to the following drawings and examples. It is to be noted that the following examples are only illustrative of the present application, and do not limit the scope of the present application. Likewise, the following examples are only some examples and not all examples of the present application, and all other examples obtained by a person of ordinary skill in the art without any inventive step are within the scope of the present application.

Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. It is explicitly and implicitly understood by one skilled in the art that the embodiments described herein can be combined with other embodiments.

Throughout the description of the present application, it is intended that the terms "mounted," "disposed," "connected," and "connected" be construed broadly and encompass, for example, a fixed connection, a removable connection, or an integral connection unless expressly stated or limited otherwise; can be mechanically connected or electrically connected; they may be directly connected or may be connected via an intermediate medium. To one of ordinary skill in the art, the foregoing may be combined in any suitable manner with the specific meaning ascribed to the present application.

The present application first proposes a sample detection method, which is applied to a sample analyzer, and referring to fig. 1 and fig. 2, fig. 1 is a schematic structural diagram of an embodiment of the sample analyzer of the present application, and fig. 2 is a schematic flow diagram of an embodiment of the sample detection method of the present application. In a first embodiment, as shown in fig. 1, a sample analyzer 10 includes a light source module 11, a sensor module 12, a processing module 13, and an optical detection module 14.

As shown in fig. 2, the sample detection method includes:

step S11: the light source module 11 emits a probe beam toward the sample.

The light source module 11 may include a light source and a lens, and the lens is disposed between the light source and a sample container for containing a sample. The sample container is made of a transparent material, such as a glass test tube or a plastic test tube, and the light source module 11 controls the light source to emit a probe light beam to the sample container, so that the probe light beam can transmit a corresponding transmitted light beam through the sample container and/or reflect a corresponding reflected light beam through the sample container.

Step S12: the sensor module 12 receives the transmitted beam of probe light transmitted through the sample and/or the reflected beam of probe light reflected therefrom.

The sensor module 12 may include a photosensor, which is disposed on an optical path of the transmitted light beam and/or the reflected light beam, and the sensor module 12 receives the corresponding transmitted light beam and/or the reflected light beam transmitted by the detection light beam through the sample container based on the photosensor, so as to convert the transmitted light beam and/or the reflected light beam into an electrical signal, and obtain optical information contained in the transmitted light beam and/or the reflected light beam.

Step S13: the processing module 13 determines whether the degree of interference of the interfering object in the sample reaches a preset degree threshold based on the transmitted and/or reflected light beams.

The processing module 13 may determine whether an interfering object exists in the sample container and whether the concentration of the interfering object is too high based on the optical information contained in the transmitted light beam and/or the reflected light beam, and if the concentrations of the interfering objects are too high, the optical detection module 14 may be prone to detection failure, and then it may be determined that the interference degree of the interfering object in the sample reaches a preset degree threshold, and conversely, if the concentrations of the interfering objects are low and are not enough to cause the optical detection module 14 to detection failure, it may be determined that the interference degree of the interfering object in the sample does not reach the preset degree threshold.

Thus, the degree of interference may refer to a concentration of the interfering substance, and the preset degree threshold may refer to a threshold concentration of the interfering substance. The interfering substance may be a specific substance present in the sample when it contains symptoms such as hemolysis, chyle, jaundice, etc., and the specific substance may cause an abnormal change in the color of the sample.

Step S14: when the interference degree does not reach the preset degree threshold, the processing module 13 controls the optical detection module 14 to detect the sample based on the optical detection method.

Wherein, when the interference degree of the interferent in the sample does not reach the preset degree threshold value, it can be determined that the optical detection module 14 can successfully obtain the detection result of the sample based on the optical detection method, and the detection failure of the optical detection method due to the existence of the interferent cannot be caused, so that the optical detection module 14 can be controlled to detect the sample based on the optical detection method, the detection success rate is higher, the risk that the sample needs to be collected again for secondary detection is reduced, and the detection efficiency of the sample detection is improved.

For example, in an application scenario, referring to fig. 3, fig. 3 is a schematic structural diagram of an embodiment of a light source module and a sensor module, as shown in fig. 3, the light source module 11 includes a light source 111 and a lens 112, the sensor module 12 includes a photosensor 121, a sample is placed in a sample container 15, the lens 112 is disposed between the light source 111 and the sample container, and the photosensor 121 is disposed on an optical path of the transmitted light beam, that is, the light source 111 and the photosensor 121 are located on two sides of a cuvette.

In another application scenario, referring to fig. 4, fig. 4 is a schematic structural diagram of another embodiment of the light source module and the sensor module, as shown in fig. 4, the light source module 11 includes a light source 111 and a lens 112, the sensor module 12 includes a photosensor 121, the specimen is placed in the specimen container 15, the lens 112 is disposed between the light source 111 and the specimen container, and the photosensor 121 is disposed on the optical path of the reflected light beam, that is, the light source 111 and the photosensor 121 are located on the same side of the cuvette.

Specifically, the photosensor 121 may be a color sensor operable to receive red, green, and blue light beams in the transmitted and/or reflected light beams, respectively.

Different from the prior art, the technical scheme of this application is through launching the detecting beam to the sample to whether the interference degree of high-end interferent reaches preset degree threshold value in the sample is confirmed based on corresponding transmission light beam and/or reflected light beam, and only adopt the optical detection method to detect the sample when the interference degree does not reach preset degree threshold value, the condition that fails when adopting the optical detection method to detect the sample, and need the secondary to gather the sample in order to carry out secondary sample detection takes place, has improved sample detection efficiency.

The present application also proposes a sample detection method, which is applied to a sample analyzer, and referring to fig. 5 and fig. 6, fig. 5 is a schematic structural diagram of another embodiment of the sample analyzer of the present application, and fig. 6 is a schematic flow chart of another embodiment of the sample detection method of the present application. In a second embodiment, as shown in fig. 5, the sample analyzer 10 includes a light source module 11, a sensor module 12, a processing module 13, an optical detection module 14, and a magnetic bead detection module 16.

As shown in fig. 6, the sample detection method includes:

step S21: the light source module 11 emits a probe beam toward the sample.

Step S22: the sensor module 12 receives the transmitted beam of probe light transmitted through the sample and/or the reflected beam of probe light reflected therefrom.

Step S23: the processing module 13 determines whether the degree of interference of the interfering object in the sample reaches a preset degree threshold based on the transmitted and/or reflected light beams.

Step S24: when the interference degree does not reach the preset degree threshold, the processing module 13 controls the optical detection module 14 to detect the sample based on the optical detection method.

Step S25: when the interference degree reaches a preset degree threshold value, the processing module 13 controls the magnetic bead detection module 16 to detect the sample based on a magnetic bead method.

When the interference degree of the interferent in the sample reaches the preset degree threshold value, it can be determined that the optical detection module 14 cannot successfully acquire the detection result of the sample based on the optical detection method, so that the magnetic bead detection module 16 can be controlled to detect the sample based on the magnetic bead method, the magnetic bead method cannot receive the interference of the interferent although the cost is high, the detection result of the sample can be successfully acquired, the risk that the sample needs to be acquired secondarily for secondary detection is reduced, and the detection efficiency of the sample detection is further improved.

It should be noted that steps S21-S24 in the second embodiment correspond to steps S11-S14 in the first embodiment, and are not described herein again.

Step S24 and step S25 are not limited in order, and step S24 is executed when it is determined that the degree of interference does not reach the preset degree threshold value based on step S23, and step S25 is executed when it is determined that the degree of interference reaches the preset degree threshold value based on step S23.

In an embodiment, referring to fig. 9, fig. 9 is a schematic structural diagram of a sample analyzer according to still another embodiment of the present application, and as shown in fig. 9, the sample analyzer includes a light source module 41, a sensor module 42, a sample application module 43, a processing module (not shown), a reaction cup module 44, a reagent module 45, a sample module 46, a sampling module 47, an optical detection module 48, and a magnetic bead detection module 49.

The sample module 46 is used to store the collected samples. The reagent module 45 is used to store reagents to be reacted with the sample. The cuvette module 44 stores a number of empty cuvettes. The sample adding module 43 is used for temporarily storing the reaction cup containing the sample when judging whether the interference degree of the interferent in the sample reaches a preset degree threshold. The reagent module 45 is used for storing reagents required by an optical detection method or a magnetic bead method.

The sampling module 47 is used to take an empty cuvette from the cuvette module 44 and place it in the sample application module 43, and to take a sample from the sample module 46 and place it in a cuvette located in the sample application module 43. The light source module 41 is used to emit a probe beam toward the sample. The sensor module 42 is configured to receive a transmitted beam and/or a reflected beam of the probe beam transmitted through the sample. The processing module is used for determining whether the interference degree of the interferent in the sample reaches a preset degree threshold value based on the transmitted light beam and/or the reflected light beam.

The processing module is configured to control the optical detection module 48 to detect the sample based on an optical detection method when the interference degree does not reach the preset degree threshold. The processing module is further configured to control the magnetic bead detection module 49 to detect the sample based on an optical detection method when the interference degree reaches a preset degree threshold.

The present application further provides a sample detection method, which is applied to a sample analyzer, in a third embodiment, the sample analyzer corresponds to the sample analyzer shown in fig. 1, and the sample detection method corresponds to the sample detection method shown in fig. 2, and details thereof are not repeated here.

In an embodiment, step S12 may specifically include: the sensor module 12 receives a reflected beam reflected by a transmitted beam transmitted by the sample, and obtains the intensity of the red beam, the intensity of the green beam, and the intensity of the blue beam from the transmitted beam and/or the reflected beam.

Step S13 may specifically include: the processing module 13 determines whether the interference degree of the interfering object in the sample reaches a preset degree threshold value based on the light intensity of the red light beam, the light intensity of the green light beam, and the light intensity of the blue light beam.

Specifically, the photosensors within the sensor module 12 for receiving the light beams are color sensors.

The sensor module 12 receives the transmitted light beam and/or the reflected light beam transmitted and/or reflected by the detection light beam from the sample, and obtains the corresponding light intensity of the red light beam, the light intensity of the green light beam, and the light intensity of the blue light beam from the transmitted light beam and/or the reflected light beam, which may specifically include: the sensor module 12 receives the transmitted light beam and/or the reflected light beam transmitted and/or reflected by the probe beam from the sample, and obtains the light intensity of the red light beam, the light intensity of the green light beam, and the light intensity of the blue light beam from the transmitted light beam and/or the reflected light beam based on the color sensor.

Optionally, the processing module 13 determines whether the interference degree of the interfering object in the sample reaches a preset degree threshold value based on the light intensity of the red light beam, the light intensity of the green light beam, and the light intensity of the blue light beam, and specifically may include:

the processing module 13 determines the color of the sample based on the light intensity of the red light beam, the light intensity of the green light beam, and the light intensity of the blue light beam.

The processing module 13 determines that the interference degree of the interfering object in the sample reaches the preset degree threshold when the color of the sample reaches the preset threshold, and determines that the interference degree of the interfering object in the sample does not reach the preset degree threshold when the color of the sample does not reach the preset threshold.

Specifically, the color of the sample is determined by obtaining the light intensity of the red light beam in the transmitted light beam and/or the reflected light beam, the light intensity of the green light beam in the transmitted light beam and/or the reflected light beam, and the light intensity of the blue light beam in the transmitted light beam and/or the reflected light beam, and based on a preset synthesis algorithm and the light intensities of the three color light beams, whether the color is within a color threshold range or not can be determined based on the color of the sample, if so, the interference degree of the interferent in the sample can be determined not to reach a preset degree threshold, and if not, the interference degree of the interferent in the sample can be determined to reach the preset degree threshold.

Further, the color sensor includes a red filter, a green filter, a blue filter, and an optical intensity sensor.

The sensor module 12 obtains the light intensity of the red light beam, the light intensity of the green light beam, and the light intensity of the blue light beam from the transmitted light beam and/or the reflected light beam based on the color sensor, and may specifically include:

the sensor module 12 filters out the red light beam in the transmitted light beam and/or the reflected light beam through a red filter, and obtains the light intensity of the red light beam through a light intensity sensor. The sensor module 12 filters out the green light beam in the transmitted light beam and/or the reflected light beam through a green filter, and obtains the light intensity of the green light beam through a light intensity sensor. The sensor module 12 filters out the blue light beam in the transmitted light beam and/or the reflected light beam through the blue filter, and obtains the light intensity of the blue light beam through the light intensity sensor.

It should be noted that the color sensor may be a device in which a silicon photodiode and a current-to-frequency converter are integrated in a CMOS (Complementary Metal Oxide Semiconductor) circuit, and a red, green and blue three-color filter is provided, so that the color sensor only receives light beams of corresponding colors to convert the light beams into electrical signals when filters of different colors are selected.

In an embodiment, step S14 may specifically include:

when the interference degree does not reach the preset degree threshold, the processing module 13 re-executes step S12 and step S13. When the number of times that the interference degree continuously does not reach the preset degree threshold reaches the preset number threshold, the processing module 13 controls the optical detection module 14 to detect the sample based on the optical detection method.

Specifically, in order to avoid the situation that the interference level in the sample is considered to be insufficient based on the determination result obtained in step S13 because the interference in the sample moves to a position outside the optical path of the probe beam, step S12 and step S13 may be repeatedly executed when the interference level does not reach the preset level threshold, and step S12 and step S13 may be repeatedly executed each time the interference level is detected to not reach the preset level threshold. If the interference degree is detected to be less than the preset degree threshold value for a plurality of times continuously, and the times reach the preset time threshold value, the controllable interference degree is not greater than the preset degree threshold value.

Based on the mode, the success rate of the optical detection method can be further improved, and the efficiency of the sample detection method is improved.

In addition, when the interference degree does not reach the preset degree threshold, the processing module 13 re-executes step S12 and step S13, which may specifically include: when the interference degree does not reach the preset degree threshold, the processing module 13 waits for a preset time period and re-executes step S12 and step S13.

Based on the mode, after the substance in the sample moves sufficiently, the detection of the interference degree can be carried out, so that the probability of detecting the possible interferent in the sample is improved, and the reliability of the sample detection method is improved.

Optionally, as shown in fig. 5, the sample analyzer 10 further comprises a magnetic bead detection module 16.

The sample detection method further comprises:

when the frequency that the interference degree does not reach the preset degree threshold value continuously does not reach the preset frequency threshold value, the processing module 13 controls the magnetic bead detection module 16 to detect the sample based on a magnetic bead method.

Specifically, for example, if the interference degree is detected three times in succession and does not reach the preset degree threshold, and the total number of times of continuously executing steps S12 and S13 reaches four times, the interference degree is detected to reach the preset degree threshold, and if the preset degree threshold is 4 times or more than 4 times, it can be considered that the number of times of continuously not reaching the preset degree threshold does not reach the preset degree threshold, and at this time, the magnetic bead detection module 16 can be controlled to detect the sample based on the magnetic bead method.

Based on the mode, when the interference degree in the sample is too high, the magnetic bead method is adopted to detect the sample, so that the situation that the sample is detected by adopting the optical detection method and fails to need secondary sample collection and detection is avoided, and the sample detection efficiency is improved.

In an embodiment, step S12 may specifically include: the sensor module 12 receives the transmitted beam of probe light transmitted through the sample and the reflected beam of probe light reflected therefrom.

Step S13 may specifically include: the processing module 13 determines whether the interference degree of the interfering object in the sample reaches a preset degree threshold value based on the transmitted light beam, and the processing module 13 determines whether the interference degree of the interfering object in the sample reaches the preset degree threshold value based on the reflected light beam. If the processing module 13 determines that the interference degree of the interfering object in the sample does not reach the preset degree threshold value based on the transmitted light beam, and the processing module 13 determines that the interference degree of the interfering object in the sample does not reach the preset degree threshold value based on the reflected light beam, the processing module 13 determines that the interference degree does not reach the preset degree threshold value.

Specifically, the sensor module 12 can receive the transmitted light beam transmitted by the probe beam through the sample and the reflected light beam reflected by the probe beam through the sample, respectively, and the processing module 13 connected to the sensor module 12 can comprehensively determine whether the interference degree of the interferent in the sample reaches the preset degree threshold according to the received transmitted light beam and reflected light beam.

When the judgment result obtained by the processing module 13 by performing data processing based on the transmitted light beam and the judgment result obtained by performing data processing based on the reflected light beam are both the interference degree of the interfering object in the sample, which does not reach the preset degree threshold, it can be directly determined that the interference degree of the interfering object in the sample does not reach the preset degree threshold.

Based on the above manner, after the processing module 13 performs data processing based on the transmitted light beam and the reflected light beam, it is finally determined that the interference degree of the interfering object in the sample does not reach the preset degree threshold, and then based on the finally determined determination result, the subsequent execution step is determined, and a suitable detection method is used to detect the sample (for example, as mentioned in the foregoing embodiment, when the interference degree does not reach the preset degree threshold, the processing module 13 controls the optical detection module 14 to detect the sample based on the optical detection method), so that the accuracy of sample detection is improved.

Optionally, after the processing module determines whether the interference degree of the interfering object in the sample reaches the preset degree threshold based on the transmitted light beam, and the processing module determines whether the interference degree of the interfering object in the sample reaches the preset degree threshold based on the reflected light beam, step S13 may further include:

if the processing module 13 determines that the interference degree of the interfering object in the sample does not reach the preset degree threshold based on the transmitted light beam, and the processing module 13 determines that the interference degree of the interfering object in the sample reaches the preset degree threshold based on the reflected light beam, or if the processing module 13 determines that the interference degree of the interfering object in the sample reaches the preset degree threshold based on the transmitted light beam, and the processing module 13 determines that the interference degree of the interfering object in the sample does not reach the preset degree threshold based on the reflected light beam, the processing module 13 re-executes the step of determining whether the interference degree of the interfering object in the sample reaches the preset degree threshold based on the transmitted light beam, and the processing module 13 determines whether the interference degree of the interfering object in the sample reaches the preset degree threshold based on the reflected light beam.

If the processing module 13 determines that the interference degree of the interfering object in the sample does not reach the preset degree threshold based on the transmitted light beam, and the processing module 13 determines that the interference degree of the interfering object in the sample reaches the preset degree threshold based on the reflected light beam, or if the processing module 13 determines that the interference degree of the interfering object in the sample reaches the preset degree threshold based on the transmitted light beam, and the processing module 13 determines that the interference degree of the interfering object in the sample does not reach the preset degree threshold based on the reflected light beam, the processing module 13 determines that the interference degree reaches the preset degree threshold.

Specifically, in the determination result obtained by the processing module 13 performing data processing based on the transmitted light beam and the determination result obtained by performing data processing based on the reflected light beam, if there is one determination result that the interference degree of the interfering object in the sample reaches the preset degree threshold and another determination result that the interference degree of the interfering object in the sample does not reach the preset degree threshold, the final determination of the determination result is not temporarily performed, but the processing module 13 is controlled to perform the step of determining whether the interference degree of the interfering object in the sample reaches the preset degree threshold again based on the transmitted light beam and determining whether the interference degree of the interfering object in the sample reaches the preset degree threshold again based on the reflected light beam, so that the processing module 13 performs transmission determination of the interference degree of the interfering object in the sample again based on the received light beam and the reflected light beam.

Then, if the processing module 13 performs the above step of determining whether the interference degree of the interfering object in the sample reaches the preset degree threshold based on the transmitted light beam for the second time, and the processing module 13 determines whether the interference degree of the interfering object in the sample reaches the preset degree threshold based on the reflected light beam, in two determination results obtained by the processing module 13 based on the transmitted light beam and the reflected light beam, there still exists a determination result that the interference degree of the interfering object in the sample reaches the preset degree threshold, and another determination result that the interference degree of the interfering object in the sample does not reach the preset degree threshold, it is finally determined that the interference degree of the interfering object in the sample reaches the preset degree threshold, and further, based on the finally determined determination result, it is determined that the subsequent execution step is performed to detect the sample by using an appropriate detection method (for example, as mentioned in the foregoing embodiment, when the interference degree of the processing module 13 reaches the preset degree threshold, the magnetic bead detection module 16 is controlled to detect the sample based on a magnetic bead method, so that the accuracy of sample detection is improved.

Further, if the processing module 13 determines that the interference degree of the interfering object in the sample does not reach the preset degree threshold based on the transmitted light beam, and the processing module 13 determines that the interference degree of the interfering object in the sample reaches the preset degree threshold based on the reflected light beam, or if the processing module 13 determines that the interference degree of the interfering object in the sample reaches the preset degree threshold based on the transmitted light beam, and the processing module 13 determines that the interference degree of the interfering object in the sample does not reach the preset degree threshold based on the reflected light beam, the step of determining whether the interference degree of the interfering object in the sample reaches the preset degree threshold based on the transmitted light beam by the processing module 13 is executed again, and the step of determining whether the interference degree of the interfering object in the sample reaches the preset degree threshold based on the reflected light beam by the processing module 13 may specifically include:

if the processing module 13 determines that the interference degree of the interfering object in the sample does not reach the preset degree threshold based on the transmitted light beam, and the processing module 13 determines that the interference degree of the interfering object in the sample reaches the preset degree threshold based on the reflected light beam, or if the processing module 13 determines that the interference degree of the interfering object in the sample reaches the preset degree threshold based on the transmitted light beam, and the processing module 13 determines that the interference degree of the interfering object in the sample does not reach the preset degree threshold based on the reflected light beam, then:

after the relative position between the light source module 11 and the sample is changed, the processing module 13 determines whether the interference degree of the interfering object in the sample reaches the preset degree threshold value based on the transmitted light beam, and the processing module 13 determines whether the interference degree of the interfering object in the sample reaches the preset degree threshold value based on the reflected light beam.

In particular, the sample may be contained in a transparent container, for example in a test tube.

The relative position between the light source module 11 and the sample may be changed by moving the light source module 11 to change the direction and angle of the probe beam irradiating the sample when the light source module 11 emits the probe beam to the sample, or by rotating the test tube to change the direction and angle of the probe beam irradiating the sample when the light source module 11 emits the probe beam to the sample.

In addition, the cuvette may be tilted with respect to the light source module 11, that is, the angle of the probe beam irradiated to the cuvette wall is changed, so that the transmitted beam and the reflected beam are generated by the probe beam in the following process, which are different from those generated when the probe beam is first emitted.

The step of determining whether the interference degree reaches the preset degree threshold value may be executed again by changing the relative position between the light source module 11 and the sample, so that the probe beam emitted by the light source module 11 may re-irradiate the sample in different directions and angles, and generate a new transmitted beam and a new reflected beam, and further perform the determination based on the new transmitted beam and the new reflected beam.

Based on the above manner, the obtained determination result that the interference degree of the finally determined interfering substance in the sample reaches the preset degree threshold is relatively accurate, and it can be further ensured that when the interference degree of the interfering substance in the sample reaches the preset degree threshold, the optical detection module 14 is not used for detecting the sample based on the optical detection method, so that the risk of detection errors is reduced, and the accuracy of sample detection is improved.

Optionally, after the processing module 13 determines whether the interference degree of the interfering object in the sample reaches the preset degree threshold based on the transmitted light beam, and the processing module 13 determines whether the interference degree of the interfering object in the sample reaches the preset degree threshold based on the reflected light beam, step S13 may further include:

if the processing module 13 determines that the interference degree of the interfering object in the sample reaches the preset degree threshold value based on the transmitted light beam, and the processing module 13 determines that the interference degree of the interfering object in the sample reaches the preset degree threshold value based on the reflected light beam, the processing module 13 determines that the interference degree reaches the preset degree threshold value.

Specifically, when the determination result obtained by the processing module 13 performing data processing based on the transmitted light beam and the determination result obtained by the processing module 13 performing data processing based on the reflected light beam both indicate that the interference degree of the interfering object in the sample reaches the preset degree threshold, it may be directly determined that the interference degree of the interfering object in the sample reaches the preset degree threshold.

Based on the above manner, after the processing module 13 performs data processing based on the transmitted light beam and the reflected light beam, it may be finally determined that the interference degree of the interfering substance in the sample reaches the preset degree threshold, and then based on the finally determined determination result, the subsequent execution step may be determined, and a suitable detection method may be used to detect the sample (for example, as mentioned in the foregoing embodiment, when the processing module 13 reaches the preset degree threshold, the magnetic bead detection module 16 is controlled to detect the sample based on the magnetic bead method), so that the accuracy of sample detection is improved.

The present application also provides a sample analyzer, referring to fig. 7, fig. 7 is a schematic structural diagram of a further embodiment of the sample analyzer of the present application, and the sample analyzer 20 includes a light source module 21, a sensor module 22, a processing module 23, and an optical detection module 24.

The light source module 21 is used to emit a probe beam toward the sample.

The sensor module 22 is configured to receive a transmitted beam and/or a reflected beam of the probe beam transmitted through the sample.

The processing module 23 is configured to determine whether the interference level of the interfering object in the sample reaches a preset level threshold based on the transmitted light beam and/or the reflected light beam.

The processing module 23 is further configured to control the optical detection module 24 to detect the sample based on the optical detection method when the interference degree does not reach the preset degree threshold.

The present application further provides a control device of a sample analyzer, referring to fig. 8, fig. 8 is a schematic structural diagram of an embodiment of the control device of the sample analyzer of the present application, and the control device 30 includes a light source module 31, a sensor module 32, a processing module 33, and an optical detection module 34.

The light source module 31 is used to emit a probe beam toward the sample.

The sensor module 32 is configured to receive a transmitted beam and/or a reflected beam of the probe beam transmitted through the sample.

The processing module 33 is configured to determine whether the interference level of the interfering object in the sample reaches a preset level threshold based on the transmitted light beam and/or the reflected light beam.

The processing module 33 is further configured to control the optical detection module 34 to detect the sample based on the optical detection method when the interference degree does not reach the preset degree threshold.

Different from the prior art, the technical scheme of the application is that the detection light beam is emitted to the sample, whether the interference degree of the high-end interferent in the sample reaches the preset degree threshold value or not is determined based on the corresponding transmitted light beam and/or reflected light beam, and the sample is detected by adopting the optical detection method only when the interference degree does not reach the preset degree threshold value, so that the situation that the sample fails to be detected by adopting the optical detection method and needs to be secondarily collected for secondary sample detection is avoided, and the sample detection efficiency is improved.

In the description of the present application, reference to the description of the terms "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present application. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or to implicitly indicate the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present application, "plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

Any process or method descriptions in flow charts or otherwise described herein may be understood as representing modules, segments, or portions of code which include one or more executable instructions for implementing specific logical functions or steps of the process, and the scope of the preferred embodiments of the present application includes other implementations in which functions may be executed out of order from that shown or discussed, including substantially concurrently or in reverse order, depending on the functionality involved, as would be understood by those reasonably skilled in the art of the present application.

The logic and/or steps represented in the flowcharts or otherwise described herein, such as an ordered listing of executable instructions that can be viewed as implementing logical functions, can be embodied in any computer-readable medium for use by or in connection with an instruction execution system, apparatus, or device (e.g., a personal computer, server, network device, or other system that can fetch the instructions from the instruction execution system, apparatus, or device and execute the instructions). For the purposes of this description, a "computer-readable medium" can be any means that can contain, store, communicate, propagate, or transport the program for use by or in connection with the instruction execution system, apparatus, or device. More specific examples (a non-exhaustive list) of the computer-readable medium would include the following: an electrical connection (electronic device) having one or more wires, a portable computer diskette (magnetic device), a Random Access Memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or flash memory), an optical fiber device, and a portable compact disc read-only memory (CDROM). Additionally, the computer-readable medium could even be paper or another suitable medium upon which the program is printed, as the program can be electronically captured, via for instance optical scanning of the paper or other medium, then compiled, interpreted or otherwise processed in a suitable manner if necessary, and then stored in a computer memory.

The above description is only for the purpose of illustrating embodiments of the present application and is not intended to limit the scope of the present application, and all modifications of equivalent structures and equivalent processes, which are made by the contents of the specification and the drawings of the present application or are directly or indirectly applied to other related technical fields, are also included in the scope of the present application.

Claims

1. The sample detection method is characterized by being applied to a sample analyzer, wherein the sample analyzer comprises a light source module, a sensor module, a processing module and an optical detection module;

the sample detection method comprises the following steps:

the light source module emits a detection light beam to a sample;

the sensor module receives a transmitted light beam transmitted by the detection light beam through the sample and/or a reflected light beam reflected by the detection light beam;

the processing module determines whether the interference degree of the interferent in the sample reaches a preset degree threshold value based on the transmitted light beam and/or the reflected light beam;

and the processing module controls the optical detection module to detect the sample based on an optical detection method when the interference degree does not reach the preset degree threshold value.

2. The method for detecting a sample according to claim 1, wherein the sample analyzer further comprises a magnetic bead detection module;

the sample detection method further comprises:

and the processing module controls the magnetic bead detection module to detect the sample based on a magnetic bead method when the interference degree reaches the preset degree threshold value.

3. The method for detecting the sample according to claim 1 or 2, wherein the sensor module receives the transmitted beam of the probe beam transmitted through the sample and/or the reflected beam of the probe beam reflected by the sample, and comprises:

the sensor module receives a transmitted light beam and/or a reflected light beam which are transmitted by the detection light beam through the sample, and acquires the light intensity of a corresponding red light beam, the light intensity of a corresponding green light beam and the light intensity of a corresponding blue light beam from the transmitted light beam and/or the reflected light beam;

the processing module determines whether the degree of interference of the interferent in the sample reaches a preset degree threshold based on the transmitted light beam and/or the reflected light beam, including:

the processing module determines whether the interference degree of the interferent in the sample reaches a preset degree threshold value based on the light intensity of the red light beam, the light intensity of the green light beam and the light intensity of the blue light beam.

4. The method as claimed in claim 3, wherein the determining whether the interference level of the interfering object in the sample reaches a predetermined threshold level by the processing module based on the intensity of the red light beam, the intensity of the green light beam and the intensity of the blue light beam comprises:

the processing module determines the color of the sample based on the light intensity of the red light beam, the light intensity of the green light beam and the light intensity of the blue light beam;

the processing module determines that the interference degree of the interferent in the sample reaches a preset degree threshold value when the color of the sample reaches the preset threshold value, and determines that the interference degree of the interferent in the sample does not reach the preset degree threshold value when the color of the sample does not reach the preset threshold value.

5. The sample detection method according to claim 3, wherein the sensor module includes a color sensor;

the sensor module receives the transmitted light beam and/or the reflected light beam transmitted by the sample from the detection light beam, and acquires the corresponding light intensity of the red light beam, the light intensity of the green light beam and the light intensity of the blue light beam from the transmitted light beam and/or the reflected light beam, and the sensor module comprises:

the sensor module receives a transmitted light beam and/or a reflected light beam transmitted by the detection light beam through the sample, and acquires corresponding light intensity of a red light beam, light intensity of a green light beam and light intensity of a blue light beam from the transmitted light beam and/or the reflected light beam based on the color sensor.

6. The sample detection method according to claim 5, wherein the color sensor comprises a red filter, a green filter, a blue filter, and an optical intensity sensor;

the sensor module acquires the light intensity of the corresponding red light beam, the light intensity of the corresponding green light beam and the light intensity of the corresponding blue light beam from the transmitted light beam and/or the reflected light beam based on the color sensor, and the sensor module comprises:

the sensor module filters out the red light beams in the transmitted light beams and/or the reflected light beams through the red filter, and obtains the light intensity of the red light beams through the light intensity sensor;

the sensor module filters out green light beams in the transmitted light beams and/or the reflected light beams through the green filter, and obtains the light intensity of the green light beams through the light intensity sensor;

the sensor module filters out blue light beams in the transmitted light beams and/or the reflected light beams through the blue filter, and obtains the light intensity of the blue light beams through the light intensity sensor.

7. The method according to claim 1 or 2, wherein the processing module controls the optical detection module to detect the sample based on an optical detection method when the degree of interference does not reach the preset degree threshold, and the method includes:

when the interference degree does not reach the preset degree threshold value, the processing module re-executes the step that the sensor module receives the transmitted light beam transmitted by the sample and/or the reflected light beam reflected by the sample, and the processing module determines whether the interference degree of the interferent in the sample reaches the preset degree threshold value or not based on the transmitted light beam and/or the reflected light beam;

and the processing module controls the optical detection module to detect the sample based on an optical detection method when the frequency that the interference degree continuously does not reach the preset degree threshold reaches a preset frequency threshold.

8. The method for detecting a sample according to claim 7, wherein the sample analyzer further comprises a magnetic bead detection module;

the sample detection method further comprises:

and the processing module controls the magnetic bead detection module to detect the sample based on a magnetic bead method when the frequency of the interference degree which does not reach the preset degree threshold value continuously does not reach the preset frequency threshold value.

9. The method according to claim 1 or 2, wherein the sensor module receives the transmitted beam and/or the reflected beam of the probe beam transmitted through the sample, and comprises:

the sensor module receives a transmitted light beam transmitted by the detection light beam through the sample and a reflected light beam reflected by the detection light beam;

the processing module determines whether the interference degree of the interferent in the sample reaches a preset degree threshold value based on the transmitted light beam, and determines whether the interference degree of the interferent in the sample reaches the preset degree threshold value based on the reflected light beam;

if the processing module determines that the interference degree of the interference object in the sample does not reach the preset degree threshold value based on the transmitted light beam, and the processing module determines that the interference degree of the interference object in the sample does not reach the preset degree threshold value based on the reflected light beam, the processing module judges that the interference degree does not reach the preset degree threshold value.

10. The specimen detection method according to claim 9, wherein after the processing module determines whether the degree of interference of the interfering substance in the specimen reaches a preset degree threshold based on the transmitted light beam and determines whether the degree of interference of the interfering substance in the specimen reaches a preset degree threshold based on the reflected light beam, the specimen detection method further comprises:

if the processing module determines that the interference degree of the interferent in the sample does not reach a preset degree threshold value based on the transmitted light beam, and the processing module determines that the interference degree of the interferent in the sample reaches the preset degree threshold value based on the reflected light beam, or if the processing module determines that the interference degree of the interferent in the sample reaches the preset degree threshold value based on the transmitted light beam, and the processing module determines that the interference degree of the interferent in the sample does not reach the preset degree threshold value based on the reflected light beam, re-executing the step of determining whether the interference degree of the interferent in the sample reaches the preset degree threshold value based on the transmitted light beam, and the processing module determines whether the interference degree of the interferent in the sample reaches the preset degree threshold value based on the reflected light beam;

if the processing module determines that the interference degree of the interference object in the sample does not reach the preset degree threshold value based on the transmission light beam, and the processing module determines that the interference degree of the interference object in the sample reaches the preset degree threshold value based on the reflection light beam, or if the processing module determines that the interference degree of the interference object in the sample reaches the preset degree threshold value based on the transmission light beam, and the processing module determines that the interference degree of the interference object in the sample does not reach the preset degree threshold value based on the reflection light beam, the processing module determines that the interference degree reaches the preset degree threshold value.

11. The specimen detection method according to claim 9, wherein after the processing module determines whether the degree of interference of the interfering substance in the specimen reaches a preset degree threshold based on the transmitted light beam and determines whether the degree of interference of the interfering substance in the specimen reaches a preset degree threshold based on the reflected light beam, the specimen detection method further comprises:

if the processing module determines that the interference degree of the interference object in the sample reaches a preset degree threshold value based on the transmitted light beam, and the processing module determines that the interference degree of the interference object in the sample reaches the preset degree threshold value based on the reflected light beam, the processing module judges that the interference degree reaches the preset degree threshold value.

12. A sample analyzer is characterized by comprising a light source module, a sensor module, a processing module and an optical detection module;

the light source module is used for emitting a detection light beam to a sample;

the sensor module is used for receiving a transmitted light beam transmitted by the detection light beam through the sample and/or a reflected light beam reflected by the detection light beam;

the processing module is used for determining whether the interference degree of the interferent in the sample reaches a preset degree threshold value based on the transmitted light beam and/or the reflected light beam;

the processing module is further used for controlling the optical detection module to detect the sample based on an optical detection method when the interference degree does not reach the preset degree threshold value.

13. The control device of the sample analyzer is characterized by comprising a light source module, a sensor module, a processing module and an optical detection module;