CN114793792B - A method for restoring pollen fertility of different fertility populations of BNS series thermosensitive genic male sterile wheat - Google Patents

Abstract

The invention discloses a method for restoring fertility of BNS series temperature-sensitive genic male sterile wheat pollen, which utilizes a MeJA solution with the concentration of 50-200 mu mol/L or a DIECA solution with the concentration of 5 mu mol/L-30mmol/L to carry out spraying treatment on BNS series temperature-sensitive genic male sterile wheat sterile population or a sterile population plant, and other conditions refer to a general field cultivation management mode. The invention utilizes the MeJA solution or the DIECA solution to spray and treat, can promote the pollen fertility recovery of different fertility groups of BNS series temperature-sensitive genic male sterile wheat, thereby reducing the seed production cost of hybrid wheat.

Description

A method for restoring pollen fertility of different fertility populations of BNS series thermosensitive genic male sterile wheat method

technical field

The invention belongs to the technical field of chemical regulation and control of pollen fertility of different fertility groups of BNS series thermosensitive genic sterile wheat, and specifically relates to a method for recovering pollen fertility of different fertility groups of BNS series thermosensitive genic sterile wheat, especially relates to a method Using methyl jasmonate (MeJA) solution or diethyldithiocarbamate (DIECA) solution to promote the recovery of pollen fertility in different fertility populations of BNS series thermosensitive genic male sterile wheat.

Background technique

The utilization of wheat photothermo-sensitive male sterility (PTSMS) is a major advance in the research of hybrid wheat. The wheat light-temperature-sensitive sterile lines discovered in my country, such as ES, C49S, ZP, BS, YS, BS and BNS, have become important test materials for hybrid wheat research.

Thermosensitive genic male sterile wheat BNS was successfully bred by Ru Zhengang in 2002, and a series of sterile lines were bred with BNS as the parent. BNS series temperature-sensitive male sterile lines have the characteristics of complete sterility and complete fertility conversion, and the changing trend of fertility is: complete abortion-semi-sterility-complete fertility. Low temperature is the main reason for affecting the fertility transformation of BNS series materials, showing low temperature sterile and high temperature fertile. The sensitive part of BNS series materials to feel the low temperature is the developing young panicle. The sensitive period of fertility conversion of BNS series materials is 5-18 days before heading, that is, from the differentiation period of pistil and stamen to the formation of tetrads. During the temperature sensitive period, when the temperature is lower than 8°C, they will be completely sterile and form sterile lines; 8 -12°C is the fertility transition temperature; when the temperature is higher than 12°C, it is completely fertile, and the fertile line is the maintainer line. In the northern part of Henan, the seeds sown from September 23 to October 17 were completely sterile and could be used to produce hybrids; all sown after November 18 were completely fertile and could be used for self-propagation of sterile line seeds. The BNS series of male sterile lines have excellent characteristics of good sterility, easy transformation and specific recovery in the main wheat producing areas of Huanghuai in my country. They are very valuable male sterile lines that can be used for hybrid wheat. In the sensitive period of fertility conversion of BNS series materials, the minimum temperature often fluctuates periodically, and its fertility stability is also disturbed and fluctuates. Fertile populations with late sowing will have difficulties in restoring fertility, resulting in a decrease in yield, which will increase The cost and risk of two-line hybrid wheat seed production pose a potential serious threat to the application of BNS series materials in large-scale production.

Theoretically, there are many possibilities for the basic countermeasures to overcome the disturbance of the fertility and stability of BNS series materials by the periodic fluctuation of the minimum temperature. One possibility is to match the sensitivity of wheat to photoperiod, and breed a double-sensitive GMS line that is sensitive to both photoperiod and low temperature, but this requires a long scientific research process to realize the possibility, and it is currently limited to theoretical analysis , is still not possible in practice. Another possibility is to use modern agricultural facilities to promote short-term temperature rise, but this requires high facilities and is difficult to meet the needs of large-scale hybrid seed production. When the sensitive period of fertility conversion has been affected by periodic temperature fluctuations, or is predicted to be affected by periodic temperature fluctuations, appropriate chemical control measures to restore fertility during the same period can also be used as a remedial measure to improve the quality of BNS series materials. Yield of late-seeding fertile populations. Combined with temperature changes, the use of MeJA and DIECA to significantly restore the pollen fertility of BNS series materials at specific developmental stages has not been reported so far.

Aiming at the different fertility populations of thermosensitive genic sterile wheat BNS series materials, screening new chemical hybridization agents with strong male fertility recovery effect and low price are used to promote the fertility recovery of BNS series materials. The effective utilization of the seeds is of great significance to reduce the cost of seed production.

Contents of the invention

The technical problem solved by the present invention is to provide a method for restoring the pollen fertility of different fertility populations of BNS series thermosensitive genic male sterile wheat, which can promote the BNS series thermosensitive genic sterile by spraying treatment with MeJA solution or DIECA solution The pollen fertility of different fertility groups of wheat is restored, thereby reducing the cost of hybrid wheat seed production.

In order to solve the above technical problems, the present invention adopts the following technical scheme: a method for restoring the pollen fertility of BNS series thermosensitive genic male sterile wheat, which is characterized in that: in the main producing area of Huanghuai wheat, the BNS series thermosensitive genic male sterile wheat Under the sowing conditions of the sterile period, that is, from September 23 to October 17, the sterile population of BNS series thermosensitive genic male sterile wheat was sown, and the BNS series thermosensitive genic male sterile wheat was sown after November 18 to form the BNS series Fertile Population of Thermosensitive Genic Male Sterile Wheat;

For BNS series thermosensitive genic male sterile wheat populations, during the period of 5-8 days from the differentiation stage of the pistil primordium to the interval period, when the daily average temperature is lower than 8°C; During 6-13 days, when the daily average temperature is lower than 10°C; from before meiosis to the tetrad stage, which lasts 3-5 days, when the daily average temperature is lower than 10°C, BNS series thermosensitive genic male sterile wheat plants Spraying treatment of exogenous methyl jasmonate solution, wherein the spraying concentration of methyl jasmonate solution is 50-200 μmol/L, and the spraying amount of methyl jasmonate solution is 50-80 L/mu;

For fertile populations of BNS series thermosensitive genic male sterile wheat, during the period of 5-8 days from the differentiation stage of the pistil primordium to the interval period, when the daily average temperature is higher than 10°C; During 6-13 days, when the daily average temperature is higher than 14°C; from before meiosis to the tetrad stage, which lasts 3-5 days, when the daily average temperature is higher than 14°C, BNS series thermosensitive genic male sterile wheat plants Spraying treatment of exogenous diethyldithiocarbamate solution, wherein the spraying concentration of diethyldithiocarbamate solution is 5μmol/L-30mmol/L, and the spraying concentration of diethyldithiocarbamate solution The application rate is 50-80L/mu.

The above two groups of reagents can be sprayed with one of the above two groups of reagents according to the temperature change along with the wheat development process, and similar effects can be achieved.

It is further defined that the spraying time of the methyl jasmonate solution or the diethyldithiocarbamic acid solution is 18:00-19:00 in the evening, and corresponding to each spraying stage, it starts when the appropriate treatment temperature condition appears , Spray every 3 days.

Further defined, the spraying concentration of the methyl jasmonate solution is 100 μmol/L; the spraying concentration of the diethyldithiocarbamic acid solution is 10 mmol/L.

The invention uses chemical drugs to spray BNS series thermosensitive genital sterile wheat plants, which can make up for the defects of temperature control technology, more conveniently regulate BNS series materials, promote the recovery of pollen fertility, and reduce the cost of hybrid wheat seed production , the requirements for facilities are not very strict. Adopting the method of the invention can avoid the requirement of temperature control on facilities and labor, and the investment is less.

The present invention selects the MeJA solution that concentration is 50-200 μ mol/L or the DIECA solution that concentration is 5 μ mol/L-30mmol/L to carry out spray treatment to BNS series thermosensitive genital sterile wheat plant, other conditions refer to general field cultivation management mode namely Can. Through field cultivation, the MeJA solution with a concentration of 50-200 μmol/L or the DIECA solution with a concentration of 10 μmol/L-30mmol/L is used for spraying treatment, so as to restore the pollen fertility of different fertility groups of BNS series thermosensitive genic male sterile wheat, Reducing the cost of hybrid wheat seed production has been successful through experiments. The invention is relatively simple in operation, low in investment cost, time-saving and labor-saving, and has broad application prospects.

Detailed ways

The above-mentioned contents of the present invention are described in further detail below through the embodiments, but this should not be interpreted as the scope of the above-mentioned themes of the present invention being limited to the following embodiments, and all technologies realized based on the above-mentioned contents of the present invention all belong to the scope of the present invention.

Example 1

The temperature-sensitive genic male sterile wheat BNS366 was used as the test material, and it was sown in two sowing dates on October 12 and December 2, 2020, to form a BNS366 sterile population and a fertile population. The test site is the wheat experimental field of Henan Normal University, the soil is sandy loam, and it is planted and managed according to the general field cultivation method. From the development of stamens of thermosensitive genic male sterile wheat BNS366 to the stage of pistil primordium differentiation and ending at the tetrad stage, according to the weather forecast and actual temperature monitoring, the BNS366 population formed by sowing on October 12, 2020 (normal autumn Sterile colonies sown) were sprayed with MeJA solution, and BNS366 colonies (fertile colonies sown late) formed on December 2, 2020 were sprayed with DIECA solution.

Between March 7 and 10, 2021, the daily average temperatures are: 3.5°C (March 7), 5.9°C (March 8), 9.2°C (March 9) and 8.1°C (March 9). On March 10), the BNS366 normal autumn-sown sterile population was in the stage of pistil primordium differentiation-drug interval, and the temperature was mostly below 8°C; between March 16 and March 22, the daily average temperatures were : 8.3°C (March 18) and 8.0°C (March 19), 8.3°C (March 20), 9.6°C (March 21), 9.2°C (March 22), BNS366 normal autumn sowing The sterile population is in the stage of differentiation of pistil and stamen primordium-drug interval, and the temperature is below 10°C. In line with the conditions for the restoration of pollen fertility induced by MeJA solution, a total of 4 spraying treatments were set up on March 7, March 10, March 19 and March 22, and the concentration of MeJA solution was sprayed at 100 μmol/L.

Between March 23 and April 2, 2021, the daily average temperatures are: 15.2°C (March 25), 13.8°C (March 26), 15.1°C (March 27), 16.0°C (March 28), 15.6°C (March 29), 14.8°C (March 30), 13.8°C (March 31) and 14.5°C (April 1), BNS366 late-sowed fertile groups were in the In the drug interval-tetrad stage, the temperature is mostly higher than 14°C, which is in line with the conditions for DIECA solution to induce the recovery of pollen fertility. A total of three spraying treatments were set on March 25, March 28 and March 31. Apply a DIECA solution with a concentration of 10mmol/L.

The above two groups of treatments were all sprayed with water as the control (CK). The spraying amount is 50-80L/mu. The spraying time is 18:00-19:00 in the evening. Repeat 3 times. The pollen fertility rate and seed setting rate of each treatment were detected (domestic law and international law).

Pollen fertility rate (%)=[number of fertile pollen/(number of fertile pollen+number of sterile pollen)]×100%.

Seed setting rate (%)=(number of florets at the base/number of spikelets×2)×100% (domestic method);

Seed setting rate (%)=(number of grains per ear/number of spikelets×2)×100% (international law).

See Table 1 for the results of the fertility test of the BNS366 male sterile line.

Table 1 Fertility comparison of thermosensitive genic male sterile wheat BNS366 sterile population and fertile population under different treatments

Note: ** and * after the data represent the differences at the 0.01 and 0.05 levels between each treatment in the same column and the corresponding control, respectively.

Experiments have shown that: referring to the general field cultivation management model, spraying MeJA solution with a treatment concentration of 100 μmol/L can promote the recovery of pollen fertility of the sterile population of thermosensitive genic male sterile wheat BNS366, and spraying a treatment concentration of 10 mmol/L DIECA solution can promote The pollen fertility of BNS366 fertile population was restored, and the seed setting rate of saturated pollination was still consistent with that of the control, thus proving that this new technology of promoting the recovery of pollen fertility of different fertility populations of thermosensitive genic male sterile wheat BNS366 can indeed overcome the adverse effects of temperature fluctuations. Fertility interference, reducing the cost of hybrid wheat seed production.

The above embodiments describe the basic principles, main features and advantages of the present invention. Those skilled in the industry should understand that the present invention is not limited by the above-mentioned embodiments, and that described in the above-mentioned embodiments and the specification only illustrates the principle of the present invention, and the present invention also has various aspects without departing from the scope of the principle of the present invention. Changes and improvements, these changes and improvements all fall within the protection scope of the present invention.

Claims (3)

1. A method for recovering pollen fertility of different fertility populations of BNS series thermosensitive genic male sterile wheat, characterized in that: in the Huanghuai wheat main producing area, the BNS series thermosensitive genic male sterile wheat is sown under the condition of sterility Next, sow from September 23 to October 17 to form a sterile population of BNS series thermosensitive genic male sterile wheat, and sow BNS series thermosensitive genic male sterile wheat after November 18 to form BNS series thermosensitive genic male sterile wheat fertile groups; For BNS series of thermosensitive genic sterile wheat sterile populations, during the period from the differentiation stage of the pistil primordium to the interval period, which lasted 5-8 days, when the daily average temperature was lower than 8 ℃; , lasted 6-13 days, when the daily average temperature was lower than 10 ℃; from premeiosis to tetrad stage, lasted 3-5 days, when the daily average temperature was lower than 10 ℃, BNS series thermosensitive genic male sterility Wheat plants are sprayed with exogenous methyl jasmonate solution, wherein the spraying concentration of methyl jasmonate solution is 50-200 μmol/L, and the spraying amount of methyl jasmonate solution is 50-80 L/mu; For the fertile populations of BNS series thermosensitive genic male sterile wheat, during the period of 5-8 days from the differentiation stage of the pistil primordium to the interval period, when the daily average temperature is higher than 10 ℃; , lasted 6-13 days, when the daily average temperature was higher than 14 ℃; from pre-meiosis to tetrad stage, lasted 3-5 days, when the daily average temperature was higher than 14 ℃, BNS series thermosensitive genic male sterility Wheat plants are sprayed with exogenous diethyldithiocarbamate solution, wherein the spray concentration of diethyldithiocarbamate solution is 5 μmol/L-30 mmol/L, diethyldithiocarbamate The spraying rate of carbamic acid solution is 50-80 L/mu. 2. the method for the recovery of pollen fertility of different fertility populations of BNS series thermosensitive genic male sterile wheat according to claim 1, is characterized in that: the methyl jasmonate solution or the diethyldithiocarbamic acid solution The spraying time is 18:00-19:00 in the evening, and corresponding to each spraying stage, the spraying treatment is carried out every 3 days since the emergence of suitable treatment temperature conditions. 3. the method for the recovery of pollen fertility of BNS series thermosensitive genic male sterile wheat different fertility populations according to claim 1, is characterized in that: the spraying concentration of described methyl jasmonate solution is 100 μ mol/L; The spray concentration of the diethyldithiocarbamic acid solution is 10 mmol/L.