CN114788859A

CN114788859A - Steam equipment multi-vitamin for adjuvant therapy of Acquired Immune Deficiency Syndrome (AIDS), and preparation method and application thereof

Info

Publication number: CN114788859A
Application number: CN202210494215.8A
Authority: CN
Inventors: 何新桥
Original assignee: Changde Jizhi Biotechnology Co ltd
Current assignee: Changde Jizhi Biotechnology Co ltd
Priority date: 2022-05-05
Filing date: 2022-05-05
Publication date: 2022-07-26

Abstract

The invention relates to the technical field of biomedicine, in particular to a steam device multi-vitamin for adjuvant therapy of AIDS (acquired immune deficiency syndrome) and a preparation method and application thereof; the raw materials of the multi-vitamin comprise: lentinan, cordyceps sinensis, dendrobium officinale, cooked rhubarb, L-carnitine and sodium bicarbonate. The invention extracts 'multivitamin' from various traditional Chinese medicines, plants and foods, contains various vitamins, trace elements, abundant proteins and mineral substances, can quickly balance nutrition, supplement energy, enhance immunity, kill bacteria and viruses by means of an own immune system and self-healing function, repair damaged cells, and promote cell regeneration capability and metabolism.

Description

Steam equipment multi-vitamin for adjuvant therapy of AIDS (acquired immune deficiency syndrome) and preparation method and application thereof

Technical Field

The invention relates to the technical field of biomedicine, in particular to a steam device multi-vitamin for adjuvant therapy of AIDS (acquired immune deficiency syndrome) and a preparation method and application thereof.

Technical Field

AIDS, acquired immunodeficiency syndrome, the causative agent is HIV. At present, AIDS becomes an important public health problem which seriously threatens the public health of China.

According to the combined evaluation of Chinese disease prevention and control center, United nations AIDS planning agency and WHO, about 125 thousands of AIDS patients survive in China by 2018, 80000 new infected persons are estimated each year, the infection rate of the whole population is about 9.0/ten thousand, and about 26.2 thousands of death cases are accumulated. Over the past 10 years, the treatment of aids has progressed considerably, with 6 major classes of over 30 antiretroviral drugs. HAART aims to maximally suppress HIV replication and thereby enhance immune function, but treatment is ineffective if the patient is unable to adhere to the treatment. Non-compliance or partial compliance with HAART treatment regimens can lead to failure of viral suppression and even worsening of disease or death due to development of resistance by the virus to antiretroviral drugs. For aids patients, long-term adherence to antiretroviral drugs is a challenge, and compliance gradually decreases with time and increased adverse drug reactions. Due to the improvement in disease control by HAART, HIV-infected individuals have a growing life span, are more susceptible to other chronic diseases such as diabetes and heart disease, due to the increasing age-related complications, reduce patient compliance with complex treatment regimens, and increase the likelihood of hospitalization and drug adverse events.

In general, current aids treatment-related studies cover: new oral and other types of long-acting antiretroviral drugs simplify strategies for treatment regimens, immune-based therapies, aids virus reservoirs, prophylactic and therapeutic vaccines, etc., but how to translate science into practice and policy remains a formidable task for aids therapy. The widespread use of effective antiretroviral drugs for treatment and prevention in the absence of effective vaccines remains a key to the suppression of the global aids epidemic. The future research on the treatment of the AIDS is expected to cover a wider range, and the research on the prevention of the AIDS virus before exposure, the prevention of the transmission of mother and infant, the study on complications including hepatitis and the like is covered, so that the better prevention and treatment are brought to the people infected by the AIDS virus, the service life of the people is prolonged, and the life quality of the people is improved.

The development of medicine to date, there are several kinds of AIDS treating medicines including chemical medicine, biological medicine, etc. and Chinese medicine as one auxiliary treatment means has some important functions, so that it is important to develop one new kind of medicine composition to raise the curative effect of chemical medicine or biological medicine and reduce its side effect.

Disclosure of Invention

Based on the reasons, the applicant obtains the multi-vitamin capable of assisting in treating AIDS through a plurality of creative efforts, wherein the raw material medicines of the multi-vitamin comprise: lentinan, cordyceps sinensis, dendrobium officinale, cooked rhubarb, L-carnitine and sodium bicarbonate. Research shows that the composition of the multi-vitamin, the multi-vitamin and the lysogenic bionic enzyme can obviously improve the drug effect of the anti-HIV drug and has the effect of assisting in treating AIDS virus.

The invention is realized by the following technical scheme.

The multivitamin for adjuvant therapy of AIDS is prepared from the following raw materials: lentinan, cordyceps sinensis, dendrobium officinale, cooked rhubarb, L-carnitine and sodium bicarbonate.

The raw materials of the multi-vitamin comprise: 4-6 parts of champignon peptide, 5-15 parts of cordyceps sinensis, 5-15 parts of dendrobium officinale, 10-20 parts of cooked rheum officinale, 2-4 parts of L-carnitine and 3-6 parts of sodium bicarbonate.

The raw materials of the multi-vitamin comprise: 5 parts of champignon peptide, 10 parts of cordyceps sinensis, 10 parts of dendrobium officinale, 15 parts of cooked rhubarb, 3 parts of L-carnitine and 4.5 parts of sodium bicarbonate.

The preparation method of the multi-vitamin comprises the following steps:

the first step is as follows: adding water into the cordyceps sinensis and the dendrobium candidum, wherein the water addition amount is 20-30 times of the weight of the cordyceps sinensis, decocting for 150 plus materials for 200 minutes, filtering by adopting a 800-mesh filter screen to obtain a mixed nutrient solution, then placing the nutrient solution into a fermentation tank, adding mucor and brown sugar for fermentation, adding the mucor with the amount of 0.25-0.65% of the weight of the mixed nutrient solution, adding the brown sugar with the amount of 5-8% of the weight of the mixed nutrient solution, taking out after fermenting for 80-100 days, adding absolute ethyl alcohol with the amount of 30-50% of the weight of the mixed nutrient solution, standing for 12-24 hours, filtering to obtain a filtrate, concentrating and drying to obtain a standby material;

the second step: adding water into the cooked rhubarb, decocting for 60-120 minutes, and filtering to obtain a first decoction filtrate, wherein the water addition amount is 4-8 times of the weight of the cooked rhubarb; decocting the residue with 3-6 times of water for 60-90 min for 2 times, filtering, mixing the filtrates, concentrating, and drying to obtain extract;

the third step: and (3) uniformly mixing the standby material obtained in the first step with the cooked rhubarb extract obtained in the second step, and adding the champignon peptide, the L-carnitine and the sodium bicarbonate to obtain the multivitamin.

A Chinese medicinal composition for adjuvant treatment of AIDS comprises multivitamin, and further comprises toxolytic enzyme.

The traditional Chinese medicine composition of claim 5, wherein the multivitamin and the toxic-dissolving biomimetic enzyme are prepared according to the following formula (6-10): (2-6) in a weight ratio.

The application of the multivitamin in preparing the medicine for assisting in treating AIDS is provided.

The application of the traditional Chinese medicine composition in preparing a medicine for adjuvant therapy of AIDS.

The raw materials of the present invention are all provided by Shenyao (Xianghe) science and technology Co.

When the multi-vitamin or Chinese medicinal composition is taken for treating AIDS patients, a small molecular water vapor treatment cabin can be used at the same time: the small molecule water vapor physical therapy cabin is internally provided with gyromagnet and far infrared rays, 4 pieces of magnetized meteorites are pasted on the cabin, and 500 g of terahertz energy stones are arranged in a steam pot. Furthermore, the micromolecule water vapor in the body treatment cabin can generate far infrared rays, terahertz and gyromagnetic wavelengths which can penetrate through cell tissues and cross continuously, can get through channels and collaterals, promote blood circulation, interact with micromolecule water and clean the cell tissues. Water and toxin-dissolving biomimetic enzyme (a toxin-dissolving biomimetic enzyme and a preparation method thereof, the invention patent number is 2021102481928, the embodiment 1) can be mixed and stirred uniformly according to the proportion of 6:4, and then added into a steam pot to be heated for 39-50 ℃. The treatment time of each water vapor is 15-25 minutes. The treatment period is 15 days.

The action and mechanism of the invention are as follows: the invention extracts 'multi-vitamin' from various rare traditional Chinese medicines, plants and foods, contains various vitamins, trace elements, abundant proteins and mineral substances, can quickly balance nutrition, supplement energy, enhance immunity, kill bacteria and viruses by means of the immune system and the self-healing function of the human body, repair damaged cells and promote the regeneration capability and metabolism of the cells. On the other hand, the method adopts micromolecule water vapor body therapy, nano-scale active toxoplase which is extracted from protein through multiple fermentation is added into micromolecule water, the water vapor body therapy is carried out on a patient, capillary pores are expanded, free radicals in the body and killed bacteria and viruses are discharged, and the nano-scale active toxoplase enters in-vivo cell tissues to assist an immune system to kill the bacteria and the viruses. By eating the multivitamins, the nutrition is supplemented, the nutrition is balanced, and the self immune function is quickly recovered.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to specific embodiments. It is to be understood that these descriptions are only illustrative and are not intended to limit the scope of the present invention. Moreover, in the following description, descriptions of well-known structures and techniques are omitted so as to not unnecessarily obscure the concepts of the present invention.

Example 1

The raw material formula of the multivitamin comprises the following components: 50g of champignon peptide, 100g of cordyceps sinensis, 100g of dendrobium officinale, 150g of cooked rhubarb, 30g of L-carnitine and 45g of sodium bicarbonate.

The preparation method of the multi-vitamin comprises the following steps:

the first step is as follows: adding water into the cordyceps sinensis and the dendrobium candidum, wherein the added water amount is 25 times of the weight of the cordyceps sinensis, decocting for 180 minutes, filtering by adopting a 800-mesh filter screen to obtain a mixed nutrient solution, putting the nutrient solution into a fermentation tank, adding mucor and brown sugar for fermentation, adding mucor in an amount of 0.45 percent of the weight of the mixed nutrient solution, adding brown sugar in an amount of 6.5 percent of the weight of the mixed nutrient solution, fermenting for 90 days, taking out, adding absolute ethyl alcohol, adding ethyl alcohol in an amount of 40 percent of the weight of the mixed nutrient solution, standing for 18 hours, filtering to obtain a filtrate, concentrating and drying to obtain a standby material;

the second step: adding water into the cooked rhubarb, decocting for 100 minutes until the water amount is 6 times of the weight of the cooked rhubarb, and filtering to obtain first decoction filtrate; decocting the residue with water for 2 times, the amount of water is 4.5 times of the weight of radix et rhizoma Rhei, decocting for 75 min, filtering, mixing the three filtrates, concentrating, and drying to obtain radix et rhizoma Rhei extract;

the third step: and (3) uniformly mixing the standby material obtained in the first step with the cooked rhubarb extract obtained in the second step, adding the champignon peptide, the L-carnitine and the sodium bicarbonate, and uniformly mixing to obtain the multivitamin.

Example 2

The raw materials of the multi-vitamin comprise: 40g of champignon peptide, 50g of cordyceps sinensis, 50g of dendrobium officinale, 100g of cooked rhubarb, 20g of L-carnitine and 30g of sodium bicarbonate.

The preparation method of the multi-vitamin comprises the following steps:

the first step is as follows: adding water into the cordyceps sinensis and the dendrobium candidum, wherein the water addition amount is 20 times of the weight of the cordyceps sinensis, decocting for 200 minutes, filtering by adopting a 800-mesh filter screen to obtain a mixed nutrient solution, putting the nutrient solution into a fermentation tank, adding mucor and brown sugar for fermentation, adding mucor in an amount which is 0.65 percent of the weight of the mixed nutrient solution, adding brown sugar in an amount which is 8 percent of the weight of the mixed nutrient solution, taking out after fermenting for 100 days, adding absolute ethyl alcohol in an amount which is 30 percent of the weight of the mixed nutrient solution, standing for 24 hours, filtering to obtain a filtrate, concentrating and drying to obtain a standby material;

the second step is that: decocting radix et rhizoma Rhei preparata in water 8 times the weight of radix et rhizoma Rhei preparata for 120 min, and filtering to obtain first decoction; decocting the residue with water for 2 times, the amount of water is 6 times of the weight of the cooked radix et rhizoma Rhei, decocting for 90 min, filtering, mixing the three filtrates, concentrating, and drying to obtain cooked radix et rhizoma Rhei extract;

Example 3

The raw materials of the multi-vitamin comprise: 60g of champignon peptide, 150g of cordyceps sinensis, 150g of dendrobium officinale, 200g of cooked rhubarb, 40g of L-carnitine and 60g of sodium bicarbonate.

The preparation method of the multi-vitamin comprises the following steps:

the first step is as follows: adding water into the cordyceps sinensis and the dendrobium officinale, wherein the water adding amount is 30 times of the weight of the cordyceps sinensis, decocting for 150 minutes, filtering by adopting a 800-mesh filter screen to obtain a mixed nutrient solution, then placing the nutrient solution into a fermentation tank, adding mucor and brown sugar for fermentation, adding mucor in an amount which is 0.25 percent of the weight of the mixed nutrient solution, adding brown sugar in an amount which is 5 percent of the weight of the mixed nutrient solution, taking out after fermenting for 100 days, adding absolute ethyl alcohol, adding ethyl alcohol in an amount which is 50 percent of the weight of the mixed nutrient solution, standing for 12 hours, filtering to obtain a filtrate, concentrating and drying to obtain a standby material;

the second step: adding water into the cooked rhubarb, decocting for 60 minutes, and filtering to obtain a first decoction filtrate, wherein the water amount is 4 times of the weight of the cooked rhubarb; decocting the residue with water for 2 times, the amount of water is 3 times of the weight of radix et rhizoma Rhei, decocting for 90 min, filtering, mixing the three filtrates, concentrating, and drying to obtain radix et rhizoma Rhei extract;

Example 4

The raw materials of the multi-vitamin comprise: 45g of champignon peptide, 65g of cordyceps sinensis, 70g of dendrobium officinale, 135g of cooked rhubarb, 25g of L-carnitine and 40g of sodium bicarbonate.

The preparation method of the multi-vitamin comprises the following steps:

the first step is as follows: adding water into the cordyceps sinensis and the dendrobium candidum, wherein the added water amount is 25 times of the weight of the cordyceps sinensis, decocting for 180 minutes, filtering by adopting a 800-mesh filter screen to obtain a mixed nutrient solution, putting the nutrient solution into a fermentation tank, adding mucor and brown sugar for fermentation, adding mucor in an amount which is 0.40 percent of the weight of the mixed nutrient solution, adding brown sugar in an amount which is 6 percent of the weight of the mixed nutrient solution, taking out after fermenting for 85 days, adding absolute ethyl alcohol in an amount which is 35 percent of the weight of the mixed nutrient solution, standing for 22 hours, filtering to obtain a filtrate, concentrating and drying to obtain a standby material;

the second step: adding water into the cooked rhubarb, decocting for 110 minutes, and filtering to obtain a first decoction filtrate, wherein the water amount is 5 times of the weight of the cooked rhubarb; decocting the residue with water for 2 times, the amount of water is 4 times of the weight of radix et rhizoma Rhei, decocting for 85 min, filtering, mixing the filtrates, concentrating, and drying to obtain radix et rhizoma Rhei extract;

Example 5

80g of the multivitamin prepared in example 1 was mixed with 20g of a lyotropic biomimetic enzyme.

Example 6

60g of the multivitamin prepared in example 1 was mixed with 40g of a toxin-solubilizing biomimetic enzyme.

Comparative example 1

The comparative example is different from example 1 only in that cordyceps sinensis is lacked, and other raw materials and preparation methods are the same as those of example 1.

Comparative example 2

The comparative example is different from the example 1 only in that dendrobium officinale is lacked, and other raw material medicaments and preparation methods are the same as the example 1.

Comparative example 3

The comparative example is different from example 1 only in that the cooked rhubarb is lacked, and other raw material medicaments and preparation methods are the same as example 1.

Test example 1

Effect on immunosuppressed mice

Test animals: kunming is a healthy male mouse.

Test drugs: lamivudine tablets (am beck biopharmaceuticals limited), examples of the application and comparative examples.

Test 1 group: lamivudine tablet group.

Test 2 groups: lamivudine tablets + group of example 1.

Test 3 groups: lamivudine tablets + example 2 group.

Run 4 groups: lamivudine tablets + example 3 group.

Test 5 groups: lamivudine tablets + example 4 group.

Test 6 groups: lamivudine tablets + example 5 group.

Test 7 groups: lamivudine tablets + example 6 group.

Test 8 groups: lamivudine tablets + comparative example 1 group.

Test 9 groups: lamivudine tablets + comparative example 2 group.

Test 10 groups: lamivudine tablets + comparative example 3 group.

The test method comprises the following steps: mice were taken, randomly grouped and weighed: the control group and the test group are respectively injected with 35 percent ethanol-dissolved cyclosporine A by the abdominal cavity for 25mg/kg/d for 3 times every other day; the next day after the last cyclosporin a administration, the test group drugs were gavaged: the lamivudine tablet group is used for intragastric administration of 45mg/kg of zidovudine once a day; test 2-test 10 groups administered zidovudine 45mg/kg +1.5g/kg of the products of the examples or comparative examples by gavage once a day for 7 consecutive days in each test group; the model control group was gavaged with saline once a day for 7 consecutive days. After fasting and water deprivation for 8 hours, the mice are anesthetized by ether, and 2mL of blood is drawn at the heart apex beating part by a 1mL syringe by adopting a heart blood sampling method, immediately placed into an EDTA (ethylene diamine tetraacetic acid) anticoagulation tube and fully mixed. Detection and analysis of CD according to the kit instructions ₄ ⁺ T cell, CD ₈ ⁺ T cell subpopulation ratio. The animals are sacrificed, thymus and spleen are picked and weighed, and spleen index and thymus index (thymus mass index and spleen mass index) are calculated according to the following formula: index (mg/g) thymus or spleen mass (mg)/body mass (g).

The test results are shown in tables 1 and 2.

TABLE 1 Effect of the groups on the mouse thymus and spleen Mass indices

Note: p <0.01, P <0.05 compared to model control group; compare # P <0.01, # P <0.05 with trial 1.

TABLE 2 CD of each test group ₄ ⁺ T cell, CD ₈ ⁺ Effect of T cell ratio

Note: p <0.01, P <0.05 compared to model control group; # P <0.01 and # P <0.05 compared to run 1.

And (4) test conclusion: (1) the spleen is the largest lymphatic organ of the animal, and the function of the spleen is closely related to the immune function of the animal; the thymus is one of the most important immune organs of animals, mainly controls the differentiation and maturation of T cells, and whether the structure and the function of the T cells are healthy or not, and is closely related to the level of the animal immunity; as can be seen from the data in table 1, the lamivudine group can increase the thymus index and spleen index, while the lamivudine group (trial 2 group-trial 7 group) in the example group of the present invention can increase the thymus index and spleen index more, and has significant difference or very significant difference compared with the lamivudine group; compared with the lamivudine group, the comparative example + lamivudine group (test 8-test 10 group) has no statistical significance, which fully shows that the product of the invention can improve the immunity together with lamivudine, further can promote the efficacy of lamivudine in treating AIDS, and has the effect of adjuvant therapy of AIDS. (2) Acquired immunodeficiency syndrome is a disease caused by HIV infection, and seriously damages the immune system of a human body, thereby seriously threatening the life of the patient. With the progress of the disease, CD ₄ ⁺ Gradual depletion or complete depletion of T cells, CD of HIV-infected patients ₄ ⁺ /CD ₈ ⁺ Significantly lower than normal. The data in Table 2 of the invention show that the lamivudine group can improve the CD of mice ₄ ⁺ /CD ₈ ⁺ And example + lamivudine group (test 2 group-test 7 group) of the present invention can significantly improve CD ₄ ⁺ /CD ₈ ⁺ A significant or very significant difference compared to the lamivudine group; compared with the lamivudine group, the comparative example + lamivudine group (test 8-test 10 group) has no statistical significance, which fully shows that the product of the invention can improve the immunity together with lamivudine, further can promote the efficacy of lamivudine in treating AIDS, and has the effect of adjuvant therapy of AIDS.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. The multivitamin for adjuvant therapy of AIDS is characterized in that raw materials of the multivitamin comprise: champignon peptide, cordyceps sinensis, dendrobium officinale, cooked rhubarb, L-carnitine and sodium bicarbonate.

2. The multivitamin of claim 1, wherein the raw materials of the multivitamin comprise: 4-6 parts of champignon peptide, 5-15 parts of cordyceps sinensis, 5-15 parts of dendrobium officinale, 10-20 parts of cooked rheum officinale, 2-4 parts of L-carnitine and 3-6 parts of sodium bicarbonate.

3. The multivitamin of claim 1, wherein the raw materials of the multivitamin comprise: 5 parts of champignon peptide, 10 parts of cordyceps sinensis, 10 parts of dendrobium officinale, 15 parts of cooked rhubarb, 3 parts of L-carnitine and 4.5 parts of sodium bicarbonate.

4. The vitamin for adjuvant treatment of AIDS according to any of claims 1-3, wherein the preparation method of the vitamin comprises:

the first step is as follows: adding water into the cordyceps sinensis and the dendrobium officinale, wherein the water adding amount is 20-30 times of the weight of the cordyceps sinensis, decocting for 150-200 minutes, filtering by adopting a 800-mesh filter screen to obtain a mixed nutrient solution, then placing the nutrient solution into a fermentation tank, adding mucor and brown sugar for fermentation, adding mucor in an amount of 0.25-0.65% of the weight of the mixed nutrient solution, adding red sugar in an amount of 5-8% of the weight of the mixed nutrient solution, taking out after fermenting for 80-100 days, adding absolute ethyl alcohol in an amount of 30-50% of the weight of the mixed nutrient solution, standing for 12-24 hours, filtering to obtain a filtrate, concentrating and drying to obtain a standby material;

the second step is that: adding water into the cooked rhubarb, decocting for 60-120 minutes, and filtering to obtain a first decoction filtrate, wherein the water addition amount is 4-8 times of the weight of the cooked rhubarb; decocting the residue with 3-6 times of water for 60-90 min for 2 times, filtering, mixing the filtrates, concentrating, and drying to obtain radix et rhizoma Rhei extract;

5. A traditional Chinese medicine composition for adjuvant therapy of AIDS comprises multivitamin, and is characterized by further comprising a toxin dissolving biomimetic enzyme.

6. The traditional Chinese medicine composition of claim 5, wherein the multivitamin and the toxin-dissolving biomimetic enzyme are prepared according to the following formula (6-10): (2-6) in a weight ratio.

7. Use of a multivitamin according to any one of claims 1 to 4 for the preparation of a medicament for the adjuvant treatment of aids.

8. The use of the Chinese medicinal composition according to claim 5 or 6 in the preparation of a medicament for the adjuvant treatment of AIDS.