CN114778866A - Automatic change detection device - Google Patents

Abstract

The application is applicable to the field of sensing and detection, and provides an automatic detection device, comprising a casing and an ultrasonic suspension mechanism, the casing includes a first channel, a second channel, a third channel and a sealing cavity, and the sealing cavity includes a detection area; One channel is used for the sample droplets to be tested to move to the detection area, the second channel is used for the detection reagent droplets to move to the detection area, and the third channel is used for the detected droplets to be discharged from the sealed cavity; the ultrasonic suspension mechanism is located in the sealing chamber. The cavity, the ultrasonic suspension mechanism is used to emit ultrasonic waves to generate an acoustic pressure field in the detection area, and the acoustic pressure field is used to suspend and move the sample droplets to be tested and the detection reagent droplets, so that the sample droplets to be tested and the detection reagent liquid droplets are suspended and moved. The droplets are mixed to form mixed droplets and suspended, and the acoustic pressure field is also used to move the detected mixed droplets to the third channel and out of the sealed cavity. The whole detection process does not require manual operation, which reduces the risk of the inspector being infected by toxic substances, the detection process is simple, and the detection efficiency is higher.

Description

An automatic detection device

technical field

The present application relates to the field of sensing detection, and more particularly, to an automatic detection device.

Background technique

The sensing detection of biomarkers is the basis of clinical medical diagnosis, biomedicine and other fields. Therefore, researchers are committed to the research of various new detection methods, and have developed various types of disease markers with high sensitivity and high detection efficiency. Detection method. However, when detecting unknown and potentially infectious toxic markers, traditional detection devices usually require manual sampling and mixing of samples to be tested with detection reagents. During the detection process, the inspectors need to implement strict isolation measures. The process is complicated, the detection efficiency is low, and if the isolation measures are not done well, it is easy for the inspectors to be infected with toxic substances.

SUMMARY OF THE INVENTION

The purpose of the embodiments of the present application is to provide an automatic detection device, which aims to solve the technical problems in the prior art that the detection process is complicated, the detection efficiency is low, and the inspectors are easily infected by toxic substances.

In order to achieve the above-mentioned purpose, the technical scheme adopted in this application is: a kind of automatic detection device is provided, including:

The housing includes a first channel, a second channel, a third channel and a sealing cavity, the sealing cavity includes a detection area, the first channel is used for the droplets of the sample to be tested to enter the detection area, and the second channel is used for the detection of reagent droplets Enter the detection area, and the third channel is used for the detected droplets to be discharged from the sealed cavity;

The ultrasonic suspension mechanism is located in the sealed cavity. The ultrasonic suspension mechanism is connected to the shell. The ultrasonic suspension mechanism is used to emit ultrasonic waves to generate an acoustic pressure field in the detection area. The acoustic pressure field is used to make the sample liquid to be tested located in the acoustic pressure field. The droplets and the detection reagent droplets are suspended and moved, so that the sample droplets to be tested and the detection reagent droplets are mixed to form mixed droplets and suspended, and the acoustic pressure field is also used to move the detected mixed droplets to the third channel and discharge the seal cavity.

In a possible design, the ultrasonic suspension mechanism includes an ultrasonic assembly, the ultrasonic assembly includes a mounting seat and a plurality of ultrasonic transducers, the plurality of ultrasonic transducers are installed on the mounting seat in an array, and the mounting seat is connected to the housing.

In a possible design, the ultrasonic emission directions of the multiple ultrasonic transducers are different, and the ultrasonic emission directions of the ultrasonic transducers all point to the detection area.

In one possible design, the mounting base has a spherical crown mounting surface, and a plurality of ultrasonic transducers are spaced apart on the mounting surface.

In a possible design, the number of ultrasonic assemblies is two, the two ultrasonic assemblies are respectively installed on opposite sides of the housing along the first direction, and the two ultrasonic assemblies are respectively located on two sides of the detection area.

In a possible design, the mounting seat is provided with a through hole, and among the two mounting seats, the through hole of one of the mounting seats communicates with the first channel, and the through hole of the other is communicated with the third channel.

In a possible design, the number of the second channels is two, which are the first reagent channel and the second reagent channel respectively, and the first reagent channel and the second reagent channel are located on opposite sides of the housing along the second direction, respectively. , the first direction and the second direction are arranged at an angle.

In a possible design, a transducer control system is also included, the transducer control system is signal-connected to each ultrasonic transducer, and the transducer control system is used to control the phase of each ultrasonic transducer.

In a possible design, the first channel and the third channel are respectively connected with a control switch, and the control switch is used to control the opening and closing of the corresponding first channel and the third channel respectively; the input end of the second channel is provided with a syringe pump , the output end of the second channel is provided with an injection needle.

In a possible design, it also includes an adjustment control system and an adjustment device, the adjustment device is signally connected to the adjustment control system, and the adjustment device is used to detect the temperature and humidity in the sealed cavity and feed back the temperature and humidity values in the sealed cavity To the adjustment control system, the adjustment control system controls the adjustment device to adjust the temperature and humidity in the sealed cavity to the set temperature range and the set humidity range respectively.

The beneficial effect of the automatic detection device provided by the present application is that compared with the prior art, the automatic detection device of the present application includes a casing and an ultrasonic suspension mechanism. The housing includes a first channel, a second channel, a third channel and a sealed cavity, the sealed cavity includes a detection area, the first channel is used for the droplets of the sample to be tested to enter the detection area, and the second channel is used for the droplets of detection reagents to enter In the detection area, the third channel is used to discharge the detected droplets out of the sealed cavity; the ultrasonic suspension mechanism is located in the sealed cavity, the ultrasonic suspension mechanism is connected to the shell, and the ultrasonic suspension mechanism is used to emit ultrasonic waves to generate an acoustic pressure field in the detection area The acoustic pressure field is used to suspend and move the sample droplets to be tested and the detection reagent droplets located in the acoustic pressure field, so that the sample droplets to be tested and the detection reagent droplets are mixed to form mixed droplets and suspend. It is used to move the mixed droplets that have completed the detection to the third channel and discharge the sealed cavity.

The automatic detection device of the present application is provided with an ultrasonic suspension mechanism, and the ultrasonic suspension mechanism can generate an acoustic pressure field, and the acoustic pressure field is used to suspend and move the sample droplets to be tested and the detection reagent droplets located in the acoustic pressure field, so as to make The sample droplet is mixed with the detection reagent droplet to form a mixed droplet and suspended. The acoustic pressure field can promote the internal stirring of the mixed droplet to mix the detection reagent with the sample to be tested. The acoustic pressure field is also used to move the detected mixed droplet to The third passage and exit the sealed cavity. The whole detection process does not require manual operation, which will greatly reduce the risk of the inspector being infected by toxic substances, the detection process is simple, and the detection efficiency is higher.

Description of drawings

In order to illustrate the technical solutions in the embodiments of the present application more clearly, the following briefly introduces the accompanying drawings that need to be used in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only for the present application. In some embodiments, for those of ordinary skill in the art, other drawings can also be obtained according to these drawings without any creative effort.

Fig. 1 is a partial structural schematic diagram of an automated detection device provided by an embodiment of the present application;

2 is an internal schematic diagram of a micro-vortex formed by a mixed droplet of an automated detection device provided by an embodiment of the present application;

3 is an internal and external schematic diagram of a mixed droplet of an automated detection device provided by an embodiment of the present application at different time points in an acoustic pressure field;

4 is a schematic structural diagram of a mounting seat of an automated detection device provided by an embodiment of the present application;

5 is a schematic diagram of the wiring between multiple ultrasonic transducers and a drive circuit of an automated detection device provided by an embodiment of the present application;

6 is a schematic diagram of a control relationship between various control systems of an automated detection device provided by an embodiment of the present application;

7 is a schematic diagram of wiring of a control module of a transducer control system of an automated detection device provided by an embodiment of the present application;

8 is a schematic wiring diagram of a voltage regulator module of a transducer control system of an automated detection device provided by an embodiment of the present application;

9 is a schematic wiring diagram of a drive circuit of a transducer control system of an automated detection device provided by an embodiment of the present application;

10 is a schematic diagram of wiring of a wireless signal transmission module of a transducer control system of an automated detection device provided by an embodiment of the present application;

11 is a schematic diagram of wiring of a working signal indicating module of a transducer control system of an automated detection device provided by an embodiment of the present application;

FIG. 12 is a schematic wiring diagram of a serial port conversion module of a transducer control system of an automated detection device provided by an embodiment of the present application.

The details of the symbols involved in the above drawings are as follows:

100, housing; 110, sealed cavity; 210, first channel; 221, first reagent channel; 222, second reagent channel; 230, third channel; 310, ultrasonic transducer; 320, mounting seat; 321, Fixed support; 322, through hole; 323, mounting surface.

Detailed ways

In order to make the technical problems, technical solutions and beneficial effects to be solved by the present application clearer, the present application will be described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application, but not to limit the present application.

It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

It is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top" , "bottom", "inside", "outside", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, only for the convenience of describing the application and simplifying the description, rather than indicating or implying the indicated A structure or element must have a particular orientation, be constructed and operate in a particular orientation, and therefore should not be construed as a limitation of the present application.

In addition, the terms "first" and "second" are only used for descriptive purposes, and should not be construed as indicating or implying relative importance or implying the number of indicated technical features. Thus, a feature defined as "first" or "second" may expressly or implicitly include one or more of that feature. In the description of the present application, "plurality" means two or more, unless otherwise expressly and specifically defined.

In order to illustrate the technical solutions described in the present application, a detailed description is given below with reference to the specific drawings and embodiments.

As shown in FIG. 1 , an embodiment of the present application provides an automatic detection device, including a housing 100 and an ultrasonic suspension mechanism. The housing 100 includes a first channel 210, a second channel, a third channel 230 and a sealed cavity 110. The sealed cavity 110 includes a detection area. The first channel 210 is used for the droplets of the sample to be tested to enter the detection area, and the second channel is used for For the detection reagent droplets to enter the detection area, the third channel 230 is used for the detected droplets to be discharged from the sealed cavity 110; the ultrasonic suspension mechanism is located in the sealed cavity 110, the ultrasonic suspension mechanism is connected to the housing 100, and the ultrasonic suspension mechanism is used to emit ultrasonic waves , to generate an acoustic pressure field in the sealed cavity 110, and the acoustic pressure field is used to suspend and move the droplets of the sample to be tested and the droplets of detection reagents located in the acoustic pressure field, so that the droplets of the sample to be tested and the droplets of the detection reagents are mixed The mixed droplets are formed and suspended, and the acoustic pressure field is also used to move the detected mixed droplets to the third channel 230 and then out of the sealed cavity 110 .

The ultrasonic suspension mechanism can generate an acoustic pressure field, which is used to suspend and move the sample droplets to be tested and the detection reagent droplets in the acoustic pressure field, so that the sample droplets to be tested and the detection reagent droplets are mixed to form a mixed liquid Drops and suspends, the acoustic pressure field can promote the internal stirring of the mixed droplets to mix the detection reagent with the sample to be tested, and the detection reagent in the mixed droplet detects the sample to be tested. After the detection is completed, the ultrasonic suspension mechanism changes the acoustic pressure field to control the detection. The completed mixed droplets move to the third channel 230 , and the detected mixed droplets are discharged out of the sealed cavity 110 through the third channel 230 . The whole detection process does not require manual operation, which will greatly reduce the risk of the inspector being infected by toxic substances, the detection process is simple, and the detection efficiency is higher.

In a possible design, the shape of the casing 100 may be a cube, a cylinder or other shapes, and the material of the casing 100 may be polymethyl methacrylate, polycarbonate, polystyrene, photosensitive resin, Plastic materials such as allyl diethylene glycol carbonate, and other materials (such as metal materials) can also be selected. In a specific embodiment, the material of the casing 100 is polymethyl methacrylate. As shown in FIG. 1 , the casing 100 is in the shape of a cube. Specifically, the casing 100 may be a closed cuboid with a length, width and height of 10cm×10cm×17cm. In an optional embodiment, the casing 100 is made of a transparent material, so that the entire casing 100 is transparent; in another specific embodiment, the main part of the casing 100 is made of a metal material, and the casing The side of 100 is provided with an observation port, the observation port is provided with a transparent observation window, the observation window is embedded in the observation port, the observation window seals the observation port, and the observation window can be made of plastic material, glass material or other materials. The above-mentioned use of a transparent material to make the casing 100 or setting an observation window on the casing 100 is beneficial for the inspector to observe the detection situation in the casing 100 from the outside of the casing 100 . Alternatively, in another optional embodiment, a camera is provided in the sealed cavity 110 , a display is provided outside the casing 100 , and an image captured by the camera is displayed on the display, so as to observe the detection situation in the sealed cavity 110 through the display.

In a possible design, the first channel 210 , the second channel and the third channel 230 may be pipes independent of the casing 100 respectively, and the pipe materials of the first channel 210 , the second channel and the third channel 230 may be adopted Plastic materials such as polyetheretherketone materials, metal materials or other materials can also be used. The side wall of the housing 100 is provided with connecting holes, and the number of the connecting holes is equal to the sum of the first channel 210, the second channel, and the third channel 230. In a specific embodiment, the first channel 210, the second channel and the The number of the third channels 230 is one, and the number of connection holes is three. The first channel 210, the second channel and the third channel 230 are respectively connected with different connection holes. The first channel 210, the second channel and the third channel The three channels 230 communicate with the sealing cavity 110 inside the housing 100 through the connecting holes, respectively. Taking the first channel 210 as an example, one end of the first channel 210 is provided with an external thread, and the interior of the connecting hole corresponding to the first channel 210 is provided with an internal thread matched with the external thread of the first channel 210 , the first channel 210 It is tightly connected with the connecting hole through internal and external threads. In order to further improve the sealing performance of the sealing cavity 110 , the connection between the first channel 210 and the connecting hole can also be sealed with sealing glue, sealing ring, etc. The connection method of the second channel, the third channel 230 and the connection hole is the same as the connection method of the first channel 210 and the connection hole, and the connection method of the first channel 210, the second channel and the third channel 230 and the corresponding connection hole can also be It is other connection methods such as welding, gluing and bonding.

In another possible design, the first channel 210 , the second channel and the third channel 230 can be directly formed into an integral structure with the housing 100 . Taking the first channel 210 as an example, a through-hole is formed on the housing 100 . The hole in the thickness of the casing 100 is the first channel 210 . The length of the first channel 210 can be equal to the thickness of the casing 100 , or at least one end of the first channel 210 can protrude from the side wall of the casing 100 For example, one end of the first channel 210 protrudes from the outer surface of the housing 100 , and the inner cavity of the first channel 210 penetrates the housing 100 , so that the outside world communicates with the sealing cavity 110 through the inner cavity of the first channel 210 . The part of the first channel 210 protruding from the outer wall of the housing 100 can be used to communicate with the liquid storage container containing the sample to be tested, for example, the first channel 210 can directly extend into the liquid storage container, or the first channel 210 The end is connected to an extension hose, and the other end of the extension hose extends into the reservoir. A pumping mechanism may be provided in the first channel 210 or the liquid storage container, and the sample to be tested in the liquid storage container is transported to the first channel 210 through the pumping mechanism.

The shape of the first channel 210 may be cylindrical, prismatic or any other shape. Both the second channel and the third channel 230 adopt the same structure as the first channel 210 .

In a possible design, the ultrasonic suspension mechanism includes an ultrasonic assembly, and the ultrasonic assembly includes a mounting seat 320 and a plurality of ultrasonic transducers 310 , and the plurality of ultrasonic transducers 310 are installed on the mounting seat 320 in an array shape, and the mounting seat 320 Connected to the housing 100 . The array form of the plurality of ultrasonic transducers 310 may be a circular array, a square array, or an array of other shapes. The parameters of the ultrasonic transducer 310 can be: diameter 10mm, height 7mm, resonant frequency 40kHz, and input voltage 20Vpp. The mounting seat 320 may be fabricated by 3D printing or other methods, and the material of the mounting seat 320 may be plastic materials such as photosensitive resin or other materials.

In a specific embodiment, the mounting seat 320 is located on one side of the housing 100 , a reflector is provided in the housing 100 relative to the opposite side of the mounting seat 320 , and the mounting seat 320 and the reflector are respectively located on both sides of the detection area, The plurality of ultrasonic transducers 310 are fixed on the mounting base 320 , and the ultrasonic emission directions of each ultrasonic transducer 310 are directed toward the reflector. The ultrasonic transducer 310 is used for transmitting ultrasonic waves, the ultrasonic waves propagate to the reflector and are reflected back to the ultrasonic transducer 310 through the reflecting surface of the reflector, so that an acoustic pressure field is formed between each ultrasonic transducer 310 and the reflector, and The detection area is located in the acoustic pressure field, and there is an upward acoustic pressure in the acoustic pressure field, which is the acoustic suspension force.

In a specific embodiment, the sample droplets to be tested enter the detection area through the first channel 210, the detection area is located in the acoustic pressure field, the sample droplets to be tested in the detection area are simultaneously affected by the acoustic levitation force and gravity, and the detection area is located in the acoustic pressure field. The acoustic levitation force on the sample droplets cancels out the gravity, so that the sample droplets to be tested are located in a microgravity environment, so the sample droplets to be tested can be suspended in the detection area. The detection reagent droplets enter the detection area through the second channel and are mixed with the sample droplets in the detection area to form mixed droplets. The detection reagent in the mixed droplets detects the sample to be tested in the mixed droplets, and the mixed droplets After the detection is completed, by changing the phase of the ultrasonic transducers 310, the acoustic pressure field between each ultrasonic transducer 310 and the reflector is changed, thereby controlling the mixed droplets to move to the third channel 230, and the mixed droplets pass through the third channel 230. The three passages 230 exit the sealed cavity 110 .

In the acoustic pressure field, the magnitude and direction of the acoustic pressure in different regions are different, so the magnitude and direction of the acoustic pressure are also different for different parts in the mixed droplet located in the acoustic pressure field. Micro-vortex stirring is formed under the action of different sizes and different directions of the acoustic pressure field, so as to promote the mixing of the sample droplets to be detected and the detection reagent droplets, so that the detection efficiency is higher. As shown in Figure 2 and Figure 3. Fig. 2 is a schematic diagram of the micro-vortex stirring formed by the mixed droplets in the acoustic pressure field. The solid line circle in Fig. 2 represents the boundary of the mixed droplet, and the arrows in Fig. 2 represent the liquid flow direction in the mixed droplet. . ⅰ, ii, iii, and iv in Fig. 3 represent different states of the same mixed droplet at different time points at the same viewing angle in the acoustic pressure field, respectively, and the mixed droplet is marked with dark gray. ⅰ means that the grey mark of the mixed droplet is on the right side at the initial position; ⅱ means that after the mixed droplet is subjected to the acoustic pressure field for a period of time, the dark grey mark of the mixed droplet moves from the right to the front, that is, the mixed droplet is on the acoustic pressure field. The pressure field rotates by a certain angle; iii means that after the mixed droplet is subjected to the acoustic pressure field for a period of time, the dark gray mark of the mixed droplet moves from the front to the left, which means that the mixed droplet rotates again in the acoustic pressure field ⅳ means that the dark gray mark of the mixed droplet moves from the left to the back after being acted on by the acoustic pressure field for a period of time, which means that the mixed droplet rotates by a certain angle in the acoustic pressure field. ⅴ, ⅵ, ⅶ, and ⅷ in Fig. 3 represent the internal morphology of the same mixed droplet at different time points in the acoustic pressure field, respectively, and the circular areas in ⅴ, ⅵ, ⅶ, and ⅷ in Fig. 3 all represent the same mixed liquid The black dots in the circular area all represent the detection reagent substance and the substance to be tested in the mixed droplet. The internal material is continuously flowing inside the mixed droplet under the action of the acoustic pressure field.

In a possible design, the surface of the mounting seat 320 for mounting the ultrasonic transducer 310 is called the mounting surface 323 , and the mounting surface 323 of the mounting seat 320 may be flat, and may be circular, square or any other shape. In a specific embodiment, the installation surface 323 is a circular plane, and the plurality of ultrasonic transducers 310 are distributed on the installation surface 323 in a circular array. For example, the plurality of ultrasonic transducers 310 are divided into multiple groups, each The number of ultrasonic transducers 310 in the groups varies. Taking 36 ultrasonic transducers 310 as an example, the 36 ultrasonic transducers 310 are divided into three groups. The number of ultrasonic transducers 310 in the first group is The number of ultrasonic transducers 310 in the second group is 12, the number of ultrasonic transducers 310 in the third group is 18, and the ultrasonic transducers 310 in each group are respectively along circular paths of different sizes. They are evenly spaced, and the centers of the three groups of circular paths all coincide with the centers of the mounting surfaces 323 . The reflective surface of the reflective plate can be a plane shape, a spherical crown or any other shape.

In a possible design, the ultrasonic emission directions of the plurality of ultrasonic transducers 310 are different, and the ultrasonic emission directions of each ultrasonic transducer 310 are all directed to the detection area. So that the intensity of the acoustic pressure field in the detection area is stronger than that of other areas in the acoustic pressure field, so that the suspension of the sample droplets, detection reagent droplets or mixed droplets located in the detection area is more stable, and the mixed droplets are suspended. Different parts of the sensor are subjected to more acoustic radiation pressures of different sizes and directions, which makes the micro-vortex agitation formed by the mixed droplets more obvious, which also promotes the mixing of the sample droplets to be tested and the detection reagent droplets. faster.

In an optional embodiment, the mounting seat 320 is a flat plate, a plurality of mounting brackets are arranged on the mounting seat 320, the number of the mounting brackets is equal to the number of the ultrasonic transducers 310 on the mounting seat 320, and the mounting bracket has an inclined surface , the slopes of each mounting bracket face the detection area. Each ultrasonic transducer 310 is correspondingly placed on the inclined surface of an installation bracket, so that the ultrasonic emission directions of each ultrasonic transducer 310 are all directed to the detection area.

In another optional embodiment, as shown in FIG. 1 , the mounting seat 320 has a spherical crown mounting surface 323 , and a plurality of ultrasonic transducers 310 are distributed on the mounting surface 323 at intervals.

In a specific embodiment, a plurality of fixed supports 321 are provided on the spherical crown mounting surface 323 of the mounting seat 320 , and the number of the fixed supports 321 is equal to that of the ultrasonic transducers 310 . They are respectively installed on a fixed support member 321 . As shown in FIG. 4 , the parameters of the mounting surface 323 are: a radius of curvature of 6 cm and a maximum diameter of 8 cm. The plurality of fixed supports 321 are distributed in an array on the installation surface 323 , and the plurality of fixed supports 321 are divided into multiple groups, and the number of the fixed supports 321 in each group is not equal, and the number of the fixed supports 321 is 36. For example, the 36 fixed supports 321 are divided into three groups, the number of the fixed supports 321 of the first group is 6, the number of the fixed supports 321 of the second group is 12, and the number of the fixed supports 321 of the third group is The number is 18, and the fixed supports 321 of each group are distributed evenly along circular paths of different sizes. The fixed supports 321 of the first group are located on the inner ring of the mounting surface 323 , the fixed supports 321 of the second group are evenly spaced on the mounting surface 323 along the second circular path, and the fixed supports 321 of the first group are distributed along the first circular path. The circular paths are evenly spaced on the inner mounting surfaces of the second set of fixed supports 321, the third set of fixed supports 321 are evenly spaced along the third circular path and distributed on the outer mounting surfaces of the second set of fixed supports 321, The center of the mounting surface 323 is located on the side of the mounting seat 320 close to the detection area, the straight line formed by the center of the mounting surface 323 and the center of the mounting surface 323 is the center line of the mounting surface 323, and the centers of the three groups of circular rails are equal to each other. Located on the center line, the first group of fixed supports 321 , the second group of fixed supports 321 and the third group of fixed supports 321 are sequentially and evenly spaced along the center line.

In a possible design, each ultrasonic transducer 310 may be fixedly connected to the mounting seat 320 , for example, the ultrasonic transducer 310 and the mounting seat 320 are connected by welding, gluing or the like. Alternatively, each ultrasonic transducer 310 can also be detachably connected to the mounting seat 320. For example, the mounting seat 320 is provided with a plurality of mounting grooves, and each ultrasonic transducer 310 is respectively snapped with the mounting groove in the form of a buckle; or , the mounting seat 320 is provided with a mounting groove with a threaded hole, and each ultrasonic transducer 310 is respectively connected with the mounting groove by means of screw fit. Each ultrasonic transducer 310 and the mounting seat 320 are connected by a detachable connection structure. When any ultrasonic transducer 310 fails, it is only necessary to remove the faulty ultrasonic transducer 310 for maintenance or replacement. interfere with other ultrasonic transducers 310 . Alternatively, each ultrasonic transducer 310 can also be hinged with the mounting seat 320 , so that the ultrasonic transducer 310 can swing relative to the mounting seat 320 . A plurality of drivers are installed on the mounting seat 320, and each ultrasonic transducer 310 is respectively connected with a driver. One end of the driver is fixed on the mounting seat 320, and the output end of the driver is connected with the ultrasonic transducer 310. The driver is used to drive the ultrasonic transducer. The transducer 310 swings relative to the mounting base 320 to generate different acoustic pressure fields, so that the suspension positions and moving trajectories of the sample droplets, detection reagent droplets or mixed droplets located in the acoustic pressure fields are more diverse. The drive may be an air cylinder, motor or other drive mechanism.

In a possible design, as shown in FIG. 1 , the number of ultrasonic components is two, and the two ultrasonic components are respectively installed on opposite sides of the housing 100 along the first direction (the direction indicated by the arrow A-B in the figure) , and two ultrasonic components are located on both sides of the detection area, for example, in the direction shown in FIG. 1 , one of the ultrasonic components is installed above the detection area, and the other ultrasonic component is installed below the detection area. The multiple ultrasonic transducers 310 in the two sets of ultrasonic assemblies are arranged in the same manner. For example, if the number of ultrasonic transducers 310 is 72, then the number of ultrasonic transducers 310 in the two sets of ultrasonic assemblies is 36, and the 36 ultrasonic transducers 310 in each ultrasonic assembly are installed in an array shape, respectively. On the corresponding mounting bases 320, the farthest distance between the two mounting bases 320 is 14 cm. The ultrasonic transducers 310 in the two sets of ultrasonic components are all facing the detection area, and emit ultrasonic waves to the detection area, and an acoustic pressure field is formed between the two sets of ultrasonic transducers 310. A standing wave is formed in the field, so that the sample droplets, detection reagent droplets or mixed droplets located in the detection area are suspended in the detection area, and by changing the phase of the ultrasonic transducer 310, the standing waves are changed in the acoustic pressure field. position, so that the sample droplets, detection reagent droplets or mixed droplets located in the detection area move with the change of the position of the standing wave. Two sets of ultrasonic components are used to transmit ultrasonic waves on both sides of the detection area and opposite to the direction of the detection area, which reduces the energy loss of ultrasonic waves in the process of propagation and reflection.

In a possible design, the automatic detection device further includes a transducer control system, the transducer control system is signal-connected to each ultrasonic transducer 310 respectively, and the transducer control system is used to control the operation of each ultrasonic transducer 310. phase. As shown in FIG. 5 , the number of ultrasonic transducers 310 is 72, and the 72 ultrasonic transducers 310 are equally divided into two groups. The number of ultrasonic transducers 310 in each group is 36. The devices 310 are sequentially numbered. The 72 circles in FIG. 5 represent 72 ultrasonic transducers 310 respectively. The numbers in the circles are the numbers of each ultrasonic transducer 310. Connect with the transducer control system, No. 37 to No. 72 are a group and are connected to the transducer control system respectively. In a specific embodiment, the transducer control system includes a controller and a driving circuit, the controller is signally connected to the driving circuit, and the driving circuit is signally connected to each ultrasonic transducer 310. Each ultrasonic transducer in FIG. 5 is connected to The lines connecting the drive circuits represent the signal connections of the 72 ultrasonic transducers 310 to the drive circuits, respectively. The signal connection can be a wireless connection (such as a Bluetooth connection) or an electrical connection (such as a cable connection), etc. The controller of the transducer control system controls the drive circuit to control the phase of each ultrasonic transducer 310 to change the ultrasonic wave. The transducer 310 emits ultrasonic waves of different frequencies and phases.

In a possible design, each mounting seat 320 is provided with a through hole 322 . Among the two mounting seats 320 , one of the through holes 322 communicates with the first channel 210 , and the other through holes 322 communicate with the third channel 210 . Channel 230 communicates. As shown in FIG. 4 , the through hole 322 of the mounting seat 320 is disposed at the center of the array of the plurality of fixed supports 321 , and the sample droplets to be tested enter the two groups of ultrasonic transducers directly from the first channel 210 through the through hole 322 of the mounting seat 320 . in the acoustic pressure field between the devices 310. The transducer control system drives the sample droplets to move to the detection area located in the acoustic pressure field by changing the phase of each ultrasonic transducer 310 and suspends them. The detection reagent droplets directly enter the detection area through the second channel and interact with the detection area. The droplets of the sample to be tested located in the detection area are mixed to form a mixed droplet, and the detection reagent in the mixed droplet detects the sample to be tested in the mixed droplet. After the detection is completed, the transducer control system changes each ultrasonic transducer. 310 , the mixed droplets are driven to move to the third channel 230 and out of the sealed chamber 110 .

In a possible design, the first channel 210 and the third channel 230 are respectively connected with a control switch, and the control switch is used to control the opening and closing of the corresponding first channel 210 and the third channel 230 respectively; the second channel input The end is connected with a syringe pump, and the output end is connected with a syringe needle. The needle of the injection needle is located in the detection area, and the injection pump is connected with the injection pump control system. The injection pump control system is used to control the injection pump to inject test agent droplets into the second channel, and the detection agent droplets enter the injection needle after passing through the second channel. The detection reagent droplets enter the detection area through the injection needle.

In a specific embodiment, after the droplet of the sample to be tested enters the detection area through the first channel 210, the droplet of the sample to be tested is suspended in the detection area by the acoustic pressure field, and the droplet of the sample to be tested is close to the needle of the injection needle and injected The needle directly injects the detection reagent droplets into the sample droplets to be tested to form mixed droplets.

In another specific embodiment, the injection needle only injects the detection reagent droplets into the detection area, and the transducer control system controls the ultrasonic transducer 310 to change the phase, so as to change the acoustic pressure field generated by the ultrasonic transducer 310, thereby The detection reagent droplets are controlled to move to the sample droplets to be tested and mixed with the sample droplets to be tested to form mixed droplets.

In a possible design, the inspector can respectively control the opening and closing of the corresponding first channel 210 and the third channel 230 by manually operating the control switch. Alternatively, each control switch is respectively connected with a switch control system, and the opening and closing of the control switch is controlled by the switch control system to control the opening and closing of the first channel 210 and the third channel 230 . The control switch may include electronically controlled switches such as solenoid valves, motor-driven switches, and the like.

In a specific embodiment, the first channel 210 is connected with an automatic sampling device, the automatic sampling device is signally connected to the switch control system, the sample to be tested is stored in the automatic sampling device, and the switch control system controls the automatic sampling device to automatically inject. The automatic sampling device can be a syringe pump or other structures that can realize automatic sampling.

In a possible design, the number of the second channels is two, which are called the first reagent channel 221 and the second reagent channel 222 respectively, and the first reagent channel 221 and the second reagent channel 222 are located along the On opposite sides of the two directions, the first direction and the second direction are arranged at an angle. In a specific embodiment, as shown in FIG. 1 , the first direction is perpendicular to the second direction (the direction indicated by the arrow C-D in the figure), the first direction is the vertical direction, and the second direction is the horizontal direction. The first reagent channel 221 and the second reagent channel 222 are respectively used for the first detection reagent droplet and the second detection reagent droplet to enter the detection area.

In a specific embodiment, the first reagent channel 221 and the second reagent channel 222 are provided with a syringe pump and an injection needle correspondingly. The input end of the first reagent channel 221 is connected to a syringe pump, the output end of the first reagent channel 221 is connected to an injection needle, the needle of the injection needle corresponding to the first reagent channel 221 is located in the detection area, and the syringe pump corresponding to the first reagent channel 221 It is used to inject the first detection reagent droplets into the first reagent channel 221 , and the first detection reagent droplets are injected into the sample droplets to be detected in the detection area through the injection needle of the first reagent channel 221 . The connection structure of the second reagent channel 222 to the syringe pump and the injection needle is the same as that of the first reagent channel 221. The syringe pump of the second reagent channel 222 is used to inject the second detection reagent droplets into the second reagent channel 222. The second reagent channel 222 is injected into the droplet of the sample to be tested in the detection area by the injection needle of the second reagent channel 222 .

In a specific embodiment, the first detection reagent droplet contains a fluorescent probe, the second detection reagent droplet contains a quenching probe, and the first detection reagent droplet and the second detection reagent droplet enter the detection area and interact with each other. After the sample droplets to be tested are mixed to form mixed droplets, different parts of the mixed droplets form micro-vortex stirring under the action of acoustic radiation pressure of different sizes and directions of the acoustic pressure field, which promotes the formation of the target probe, thereby The combination of the fluorescent probe, the quenching probe and the target probe is promoted, the quenching probe conceals the fluorescence emitted by the fluorescent probe, and the detection of the sample to be tested is realized by measuring the change value of the fluorescence intensity contained in the suspension droplet.

In a possible design, in order to meet the testing requirements, when multiple detection reagents are required, the number of the second channel may be more, for example, a third reagent channel, a fourth reagent channel, etc. may also be provided. In a specific embodiment, the third reagent channel and the fourth reagent channel are respectively located on opposite sides of the housing 100 along the third direction, and the first direction, the second direction and the third direction are respectively arranged at an angle.

In a possible design, the automatic detection device further includes an adjustment control system and an adjustment device, the adjustment device is signally connected to the adjustment control system, and the adjustment device is used to detect the temperature and humidity in the sealed cavity 110 and adjust the temperature in the sealed cavity 110 The value and the humidity value are fed back to the adjustment control system, and the adjustment control system controls the adjustment device to adjust the temperature and humidity in the sealed cavity 110 to be within the set temperature range and the set humidity range, respectively.

In a specific embodiment, the adjustment device includes a temperature adjuster and a humidity adjuster, the temperature adjuster includes a temperature sensor and a temperature adjustment structure, the temperature sensor and the temperature adjustment structure are respectively connected with the adjustment control system signal, and the temperature sensor is used to detect the sealing The temperature in the cavity 110 is determined, and the temperature value in the sealed cavity 110 is fed back to the adjustment control system, and the adjustment control system compares the data fed back by the temperature sensor with the upper limit and lower limit of the set temperature range, if the temperature sensor If the feedback data exceeds the set temperature range (ie above the upper limit or below the lower limit), the adjustment control system controls the temperature adjustment structure to adjust the temperature in the sealed cavity 110 to the set temperature range. The temperature adjusting structure may include any one or more structures capable of adjusting the temperature, such as heating wires, condensers, and the like. The humidity regulator includes a humidity sensor and a humidity regulation structure. The humidity sensor and the humidity regulation structure are respectively connected to the regulation control system with signals. The humidity sensor is used to detect the humidity in the sealed cavity 110 and feed back the humidity value in the sealed cavity 110 to the regulation control system. System, the adjustment control system compares the data fed back by the humidity sensor with the upper limit value and the lower limit value of the set humidity range respectively. If the data fed back by the humidity sensor exceeds the set humidity range, the adjustment control system controls the humidity adjustment structure to seal the cavity. The humidity in 110 is adjusted to within the set humidity range. The humidity-adjusting structure may include any one or more structures capable of adjusting humidity, such as heating wires, humidifiers, and the like.

In a possible design, as shown in Figure 6, the motorized detection device further includes a main control system, the transducer control system, the syringe pump control system, the switch control system and the adjustment control system are all signal-connected to the main control system, The main control system is controlled by the user adjustment platform. The user adjustment platform and the main control system can be connected wirelessly or electrically. The user adjustment platform sends control instructions to the main control system, and the main control system respectively controls the transducer control system and the syringe pump. The control system, the switch control system, and the regulation control system operate.

As shown in FIG. 7 to FIG. 12 , the transducer control system further includes a control module, a voltage regulator module, a wireless signal transmission mode working signal indicating module, and a serial port conversion module.

As shown in Figure 7, Figure 7 is a schematic diagram of the wiring of the control module of the transducer control system. The control module uses the MEGA328P microcontroller as the controller and is the core of the entire transducer control system. The voltage required by the MEGA328P microcontroller is 5V.

As shown in Figure 8, Figure 8 is a schematic diagram of the wiring of the voltage regulator module of the transducer control system. The LM7805 in the voltage regulator module is a three-terminal voltage regulator integrated circuit. The LM7805 converts the external input voltage into a stable 5V DC voltage to For MEGA328P microcontroller use.

As shown in Figure 9, Figure 9 is a schematic diagram of the wiring of the drive circuit of the transducer control system. L298N is the motor drive chip. The drive circuit includes two signal conditioners X1 and X2. The MEGA328P single-chip microcomputer drives the L289N motor through the PC0 interface and the PC3 interface. The chip sends a control signal. After the L289N motor driver chip receives the control signal sent by the MEGA328P single-chip microcomputer, it drives X1 and X2 to run respectively. X1 and X2 respectively drive multiple ultrasonic transducers 310 in the two sets of ultrasonic components to run and change the two sets respectively. The phases of the plurality of ultrasonic transducers 310 in the ultrasonic assembly, for example, X1 controls the plurality of ultrasonic transducers 310 in one group of ultrasonic assemblies, and X2 controls the plurality of ultrasonic transducers 310 in the other group of ultrasonic assemblies. A plurality of ultrasonic transducers 310 emit ultrasonic waves, so that an acoustic pressure field is generated between two sets of ultrasonic components, and the two sets of ultrasonic components are respectively located on both sides of the detection area. The sample droplets, detection reagent droplets or mixed droplets are suspended under the action of the suspending force in the acoustic pressure field, and the MEGA328P single-chip microcomputer controls the driving circuit to control the multiple ultrasonic transducers 310 to change the phase, thereby controlling the detection area. The sample droplets to be tested, the detection reagent droplets or the mixed droplets move.

As shown in FIG. 10, FIG. 10 is a schematic diagram of the wiring of the wireless signal transmission module of the transducer control system. The wireless signal transmission module is used for wireless control (such as Bluetooth control), and the wireless communication module is used to receive the control signal transmitted by the main control system. And the control signal emitted by the main control system is transferred to MEGA328P single-chip microcomputer for processing. The wireless communication module is also used to receive the control signal sent by the MEGA328P single-chip microcomputer and transmit the control signal sent by the MEGA328P single-chip microcomputer to the main control system.

As shown in Figure 11, Figure 11 is a schematic diagram of the wiring of the working signal indicating module of the transducer control system. When the transducer control system starts to run, the MEGA328P single-chip microcomputer sends a signal to the working signal indicating module to control the working signal indicating module. The LED light is on to indicate that the automatic detection device is running.

As shown in Figure 12, Figure 12 is a schematic diagram of the wiring of the serial port conversion module of the transducer control system. The serial port conversion module is used to connect to a computer to modify the instruction program of the transducer control system.

In a specific embodiment, the detection process is as follows:

①. Turn on the automatic detection device, and the temperature and humidity in the sealed cavity 110 are adjusted to be within the set temperature range and the set humidity range respectively by the adjustment control system;

②. The liquid droplets of the sample to be tested are automatically injected through the first channel 210 , the ultrasonic transducer 310 is turned on, and the ultrasonic transducer emits ultrasonic waves in the sealed cavity 110 to form an acoustic pressure field, so that the sample to be tested is made in the sealed cavity 110 . Droplet suspension (0.1～5μL);

③. The detection reagent droplets corresponding to the sample droplets to be tested are respectively added to the sample droplets to be tested through the first reagent channel 221 and the second reagent channel 222 to form mixed droplets. At this time, the phase of the ultrasonic transducer 310 is maintained Invariant, different parts of the mixed droplets are subjected to different acoustic pressures to form micro-vortex stirring inside, and the markers to be tested contained in the mixed droplets and the corresponding detection reagents (fluorescent probes and quenching probes) are accelerated and mixed. , which further accelerates the binding efficiency of the probe; when the mixed droplet contains the target probe, both the fluorescent probe and the quenching probe are bound to the target probe, and the quenching group of the quenching probe covers the fluorescent probe. The fluorescence emitted by the fluorophore can be detected by measuring the change value of the fluorescence intensity contained in the suspended droplet;

④. The microgravity environment formed by the resultant force of the acoustic pressure generated in the automatic detection device and the gravity of the sample droplets to be tested, the droplets of detection reagents or the mixed droplets makes the droplets of the samples to be tested, the droplets of detection reagents or mixed The droplets are suspended in the acoustic pressure field, and at the same time, due to the differences in the acoustic radiation pressure received by different parts of the sample droplets, detection reagent droplets or mixed droplets in the acoustic pressure field, taking the mixed droplets as an example, different The resultant force of the acoustic radiation pressure and the gravitational action of each part of the mixed droplet, resulting in micro-vortex stirring inside the mixed droplet, thus providing a micro-stirring environment for the further combination of the analyte and the detection reagent, improving the detection performance. efficiency;

⑤. After the mixed droplet is detected, the phase of the ultrasonic transducer 310 changes, so that the acoustic pressure field also changes. Under the continuous change of the acoustic pressure field, the mixed droplet is controlled by the resultant force of the acoustic pressure and gravity to the shell. The bottom of the body 100 migrates and exits the sealed cavity through the third channel 230 .

The above are only optional embodiments of the present application, and are not intended to limit the present application. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present application shall be included in the protection scope of the present application. Inside.

Claims (10)

1. an automatic detection device, is characterized in that, comprises: The housing includes a first channel, a second channel, a third channel and a sealed cavity, the sealed cavity includes a detection area, the first channel is used for the droplet of the sample to be tested to enter the detection area, the second The channel is used for the detection reagent droplets to enter the detection area, and the third channel is used for the detected droplets to be discharged from the sealed cavity; The ultrasonic levitation mechanism is located in the sealed cavity, the ultrasonic levitation mechanism is connected with the shell, and the ultrasonic levitation mechanism is used to emit ultrasonic waves to generate an acoustic pressure field in the detection area. In order to suspend and move the sample droplets to be tested and the detection reagent droplets located in the acoustic pressure field, the sample droplets to be tested and the detection reagent droplets are mixed to form mixed droplets and suspended , the acoustic pressure field is also used to make the mixed liquid droplet after the detection move to the third channel and discharge the sealed cavity. 2. The automatic detection device according to claim 1, wherein the ultrasonic levitation mechanism comprises an ultrasonic assembly, and the ultrasonic assembly comprises a mounting seat and a plurality of ultrasonic transducers, and the plurality of the ultrasonic transducers are in the form of an ultrasonic wave. The mounting seat is mounted on the mounting seat in an array shape, and the mounting seat is connected with the housing. 3 . The automatic detection device according to claim 2 , wherein the ultrasonic emission directions of the plurality of ultrasonic transducers are different, and the ultrasonic emission directions of the ultrasonic transducers all point to the detection area. 4 . 4 . The automatic detection device according to claim 3 , wherein the mounting seat has a spherical crown mounting surface, and a plurality of the ultrasonic transducers are distributed at intervals on the mounting surface. 5 . 5 . The automatic detection device according to claim 2 , wherein the number of the ultrasonic components is two, and the two ultrasonic components are respectively installed on opposite sides of the housing along the first direction, 6 . And the two ultrasonic components are respectively located on both sides of the detection area. 6 . The automatic detection device according to claim 5 , wherein the mounting seat is provided with a through hole, and among the two mounting seats, the through hole of one of the mounting seats is communicated with the first channel, and the through hole of the other is connected to the first channel. 7 . The through hole of the third channel communicates with the third channel. 7. The automatic detection device according to claim 5, wherein the number of the second channels is two, which are respectively a first reagent channel and a second reagent channel, the first reagent channel and the second reagent channel are respectively The two reagent channels are respectively located on opposite sides of the housing along the second direction, and the first direction and the second direction are arranged at an angle. 8 . The automatic detection device according to claim 2 , further comprising a transducer control system, the transducer control system is respectively connected with each of the ultrasonic transducers by signal, and the transducer controls the A system is used to control the phase of each of the ultrasonic transducers. 9. The automatic detection device according to claim 1, wherein the first channel and the third channel are respectively connected with a control switch, and the control switch is used to control the corresponding first channel and the third channel respectively. The opening and closing of the third channel; the input end of the second channel is provided with a syringe pump, and the output end of the second channel is provided with a syringe needle. 10 . The automatic detection device according to claim 1 , further comprising an adjustment control system and an adjustment device, the adjustment device is signally connected to the adjustment control system, and the adjustment device is used to detect the sealed cavity. 11 . The temperature and humidity in the sealed cavity are fed back to the adjustment control system, and the adjustment control system controls the adjustment device to adjust the temperature and humidity in the sealed cavity to set values respectively. within the set temperature range and within the set humidity range.

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