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CN114747429A - Sterilization method for edible fungus culture medium - Google Patents

Sterilization method for edible fungus culture medium Download PDF

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Publication number
CN114747429A
CN114747429A CN202210365118.9A CN202210365118A CN114747429A CN 114747429 A CN114747429 A CN 114747429A CN 202210365118 A CN202210365118 A CN 202210365118A CN 114747429 A CN114747429 A CN 114747429A
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CN
China
Prior art keywords
culture medium
wide
sterilizing
sterilization
steps
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Pending
Application number
CN202210365118.9A
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Chinese (zh)
Inventor
张翠娥
赵书光
范育明
李明
张亚楠
张宝玉
孙红
孙晓涛
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiangsu Lisa Industry Ltd By Share Ltd
Guannan Horticultural Technology Guidance Station
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Jiangsu Lisa Industry Ltd By Share Ltd
Guannan Horticultural Technology Guidance Station
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Application filed by Jiangsu Lisa Industry Ltd By Share Ltd, Guannan Horticultural Technology Guidance Station filed Critical Jiangsu Lisa Industry Ltd By Share Ltd
Priority to CN202210365118.9A priority Critical patent/CN114747429A/en
Publication of CN114747429A publication Critical patent/CN114747429A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost
    • A01G18/22Apparatus for the preparation of culture media, e.g. bottling devices
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/30Accessories for use before inoculation of spawn, e.g. sterilisers
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/02Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
    • A61L2/04Heat
    • A61L2/06Hot gas
    • A61L2/07Steam
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/26Accessories or devices or components used for biocidal treatment

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)

Abstract

The invention discloses a sterilization method for edible fungus culture medium, belonging to the field of culture medium sterilization method, the scheme is that firstly, the culture medium raw material is coarsely ground, then the culture medium raw material is added into a wide-mouth bottle, the filling of the culture medium raw material can be greatly simplified due to the cutting of an inclined groove, after the filling is finished, a connecting sleeve is sealed by a sealing cover, the raw material filling is finished, then the culture medium container filled with the culture medium raw material is placed into a high-pressure steam sterilization device for sterilization, external steam can be placed into the wide-mouth bottle through a waterproof curtain, the culture medium raw material is loosened by a soil loosening device, on one hand, the heat conduction is facilitated, the sterilization effect is increased, on the other hand, the subsequent cultivation of edible fungi is facilitated, the culture medium can be placed in the wide-mouth container in the culture medium sterilization process, the bottling and the taking out of the culture medium are convenient, and the sterilization efficiency of the culture medium is increased.

Description

Sterilization method for edible fungus culture medium
Technical Field
The invention relates to the field of culture medium sterilization methods, in particular to a sterilization method for an edible fungus culture medium.
Background
The edible fungi refer to edible mushrooms with large fruiting bodies, are generally called mushrooms, have extremely high edible value and medicinal value, are delicious and rich in nutrition, are often called as health foods by people, for example, the mushrooms contain various amino acids necessary for human bodies, have the effects of reducing cholesterol in blood and treating hypertension, and are found to contain substances for enhancing the anticancer capability of the human bodies, so that the mushrooms, the flammulina velutipes and the hericium erinaceus are important economic crops.
The current edible fungi cultivation technology is relatively mature and can be roughly divided into several stages of breeding, culture medium preparation and hypha growth, wherein the culture medium of edible fungi mostly contains a large amount of organic matters, which usually contain a large amount of various microorganisms, and these microorganisms often adversely affect the growth of edible fungi and affect the cultivation of edible fungi, so that the culture medium needs to be sterilized in the process of culture medium preparation.
The common sterilization method in the preparation of the edible fungus mother culture medium is a high-pressure steam sterilization method, which is the most common and thorough sterilization method, and the high temperature of high-pressure steam is utilized to inactivate microorganisms such as bacteria in the culture medium so as to meet the requirement of edible fungus cultivation, but the high-pressure steam easily permeates into the culture medium in the sterilization process to cause overhigh water content in the culture medium and influence the normal growth of edible fungi, so that in the high-pressure steam sterilization process, the perfect sealing treatment needs to be carried out on a culture medium container, the bottling and taking-out efficiency of the culture medium is influenced, and the sterilization efficiency of the culture medium is influenced.
Disclosure of Invention
1. Technical problem to be solved
Aiming at the problems in the prior art, the invention aims to provide a method for sterilizing an edible fungus culture medium, which can realize that a wide-mouth container is selected to contain the culture medium in the process of sterilizing the culture medium, so that the bottling and taking out of the culture medium are facilitated, and the sterilization efficiency of the culture medium is increased.
2. Technical scheme
In order to solve the above problems, the present invention adopts the following technical solutions.
A sterilization method for an edible fungus culture medium mainly comprises the following steps:
s1, coarse grinding of the raw materials of the culture medium, and breaking of lumps in the raw materials of the culture medium;
s2, filling raw materials, namely filling the coarsely crushed culture medium raw materials into a culture medium container, and sealing the culture medium container;
and S3, sterilizing the culture medium container filled with the culture medium raw material by high-pressure steam, placing the culture medium container into a high-pressure steam sterilizing device for sterilizing, and inactivating the microorganisms in the culture medium raw material.
Furthermore, the culture medium container comprises a wide-mouth bottle, the wide-mouth bottle is filled with an edible fungus culture medium, an opening at the upper end of the wide-mouth bottle is inserted with a connecting sleeve matched with the wide-mouth bottle, the connecting sleeve comprises a fixing part, an inclined plane groove is formed in the upper end of the fixing part, a steam through hole is fixedly formed in the bottom of the inclined plane groove and penetrates through the fixing part, a sealing cover matched with the steam through hole is inserted in the steam through hole and comprises a cover body, a steam channel is formed in the cover body and is communicated with the wide-mouth bottle and the outside, a supporting net matched with the steam channel is fixedly connected to the opening at the upper end of the steam channel, a waterproof curtain is arranged at the upper end of the supporting net, the supporting net is fixedly connected with the central part of the waterproof curtain, a plurality of tearing grooves are formed in the waterproof curtain, the edge parts of the waterproof curtain are cut into a plurality of sectors by the tearing grooves, and a soil loosening device is fixedly connected to the lower end of the fixing part, the soil loosening device comprises a power source, the power source is fixed at the lower end of a fixing part, a power output end of the power source is fixedly connected with soil loosening units matched with the power source, two adjacent soil loosening units are connected end to end, each soil loosening unit comprises a connecting rod, a mounting groove matched with the power output end of the power source is formed in one end, close to the power output end of the power source, of each connecting rod, a positioning table matched with the mounting groove is formed in one end, far away from the mounting groove, of each connecting rod, a positioning ring matched with the corresponding positioning table is clamped and connected to the outer side of each positioning table, a positioning plate matched with the positioning table is formed in the inner wall of each positioning ring in a chiseled mode, a plurality of fan blades are fixedly connected to the outer wall of each positioning ring, and a fixing cover is fixedly connected to the positioning table of the soil loosening unit located at the lowest end, can realize in the culture medium sterilization process, choose wide mouthful container for holding the culture medium for use, the bottling and the taking out of the culture medium of being convenient for increase culture medium sterilization efficiency.
Furthermore, a sealing gasket is connected between the wide-mouth bottle and the connecting sleeve, and the sealing gasket is fixedly connected with the wide-mouth bottle, so that the sealing effect between the wide-mouth bottle and the connecting sleeve is improved, external high-pressure steam is difficult to enter the wide-mouth bottle through a gap between the wide-mouth bottle and the connecting sleeve, and the culture medium filled in the wide-mouth bottle is difficult to influence.
Furthermore, a sealing rubber block is arranged between the cover body and the connecting sleeve and fixedly connected with the cover body, the sealing rubber block can adapt to the inclined plane groove, and the sealing performance between the connecting sleeve and the sealing cover is not easily influenced after the sealing cover is mounted and dismounted for many times.
Furthermore, elastic cavities are formed in the sealing rubber blocks, a plurality of filling balls are filled in the elastic cavities, the elasticity of the sealing rubber blocks is increased, the adaptability of the sealing rubber blocks is increased, and the sealing effect between the connecting sleeve and the sealing cover is increased.
Furthermore, equal fixedly connected with elastic fiber on the outer wall of a plurality of packing balls, adjacent elastic fiber twines together, increases the joint strength between the adjacent packing balls for the piece is difficult for taking place excessive deformation under the exogenic action to the sealant, is difficult for influencing the sealed effect of sealant.
Further, fixedly connected with waterproof fiber on the inner wall of tearing the groove, adjacent waterproof fiber winding forms three-dimensional space spatial structure together, under the prerequisite that does not influence the waterproof curtain activity, increases the holistic sealed effect of waterproof curtain for in external vapor is difficult for entering into wide mouthful bottle through sealed lid.
Further, a plurality of waterproof fiber all are three-dimensional heliciform, increase the stability of the three-dimensional space structure that a plurality of waterproof fiber formed, increase its water-proof effects, the coating has hydrophobic coating on a plurality of waterproof fiber's the outer wall, at the cooling in-process after accomplishing the culture medium sterilization, the water that the condensation formed easily rolls off on waterproof curtain and waterproof fiber's surface, is difficult for permeating the composition that influences the culture medium in the wide-mouthed bottle.
Furthermore, the outer edge of the upper end of the cover body is high in the center and low in the outer part, so that water drops rolling down on the waterproof curtain can directly slide from the upper end of the cover body, and the waterproof curtain is not easy to accumulate in a large amount.
Furthermore, a plurality of hairline cracks are dug at one end, close to the inner wall of the positioning ring, of each positioning plate, the strength of each positioning plate can be controlled by controlling the number and the depth of the hairline cracks, so that when the fan blades cannot rotate under the action of external force, the positioning plates can be automatically broken, the connection between the connecting rods and the fan blades in the soil loosening unit is broken, and the power source is not prone to short circuit burning due to idling.
3. Advantageous effects
Compared with the prior art, the invention has the advantages that:
in this scheme, at first carry out the corase grind to the culture medium raw materials, make it accord with the requirement of follow-up technology, later will add the culture medium raw materials through the corase grind in the wide-mouthed bottle, and because the excavation of inclined plane groove can simplify filling of culture medium raw materials by a wide margin, and after filling, utilize sealed lid to seal the adapter sleeve, accomplish the raw materials and fill, later will load the culture medium container of culture medium raw materials and put into high-pressure steam sterilization device and carry out sterilization work.
Atmospheric pressure in the in-process wide-mouth bottle internal and external environment that carries out high pressure steam sterilization all can rise, because the culture medium raw materials can produce certain gas under the high temperature effect, this limit can lead to the inside atmospheric pressure of wide-mouth bottle to rise the range and be greater than the external world, can jack-up the border position of waterproof curtain this moment, release the inside high-pressure gas of wide-mouth bottle, and in above-mentioned in-process, because the air current direction is outside in the wide-mouth bottle, consequently can not cause external a large amount of steam to enter into and pollute the culture medium raw materials in the wide-mouth bottle, and when atmospheric pressure in the wide-mouth bottle resumes to be the same with the external world, waterproof curtain resumes the former state, external steam is difficult for entering into in the wide-mouth bottle, form the protection once more.
Simultaneously, through set up the device that loosens the soil on the link sleeve, can become flexible to the culture medium raw materials, make the culture medium raw materials become fluffy relatively, the heat conduction of being convenient for on the one hand, increase the sterilization effect, on the other hand also be convenient for subsequent edible mushroom's cultivation, it is special, the device that loosens the soil in this scheme has a plurality of units concatenations of loosening the soil to form, be convenient for subsequent maintenance and repair, the quantity and the degree of depth through the capillary crack of the unit of controlling the loosening the soil come the intensity of control locating plate simultaneously, make the fan piece when receiving unable rotation under the exogenic action, the locating plate can split by oneself, the disconnection loosens the soil and is connected between connecting rod and the fan piece in the unit, make the power supply be difficult for burning out because of idle circuit.
Drawings
FIG. 1 is a schematic diagram of the sterilization method of the edible fungus culture medium according to the present invention;
FIG. 2 is an exploded view of the main structure of the edible fungus culture medium containing device of the present invention;
FIG. 3 is a schematic structural view of the edible fungus culture medium containing device of the present invention;
FIG. 4 is a side cross-sectional view of the edible mushroom culture medium holding device of the present invention;
FIG. 5 is a schematic view of a connecting sleeve structure of the present invention;
FIG. 6 is a corrosion diagram of the seal cap of the present invention;
FIG. 7 is a bottom view of the seal cap of the present invention;
FIG. 8 is a side sectional view of the seal cap of the present invention;
FIG. 9 is an exploded view of the principal result of the ripping apparatus of the present invention, with the exception of a power source;
fig. 10 is a schematic structural view of a ripping unit according to the present invention.
The reference numbers in the figures illustrate:
the device comprises a wide-mouth bottle 1, a connecting sleeve 2, a fixing part 201, an inclined plane groove 202, a steam through hole 203, a sealing gasket 3, a sealing cover 4, a cover body 401, a steam channel 402, a sealing rubber block 403, a filling ball 404, elastic fibers 405, a supporting net 406, a waterproof curtain 407, a tearing groove 408, waterproof fibers 409, a soil loosening device 5, a power source 501, a connecting rod 502, a mounting groove 503, a positioning table 504, a positioning ring 505, a positioning plate 506, a fan 507 and a fixing cover 508.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention; it is to be understood that the embodiments described are merely exemplary embodiments, rather than exemplary embodiments, and that all other embodiments may be devised by those skilled in the art without departing from the scope of the present invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom", etc. indicate orientations or positional relationships based on orientations or positional relationships shown in the drawings, which are merely for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted", "provided", "fitted/connected", "connected", and the like, are to be interpreted broadly, such as "connected", which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; the connection can be direct connection or indirect connection through an intermediate medium, and can be communication inside the adaptive model element. The specific meanings of the above terms in the present invention can be understood in a specific case to those of ordinary skill in the art.
Example 1:
referring to fig. 1, a method for sterilizing an edible fungus culture medium mainly includes the following steps:
s1, coarse grinding of the raw materials of the culture medium, and breaking of lumps in the raw materials of the culture medium;
s2, filling raw materials, namely filling the coarsely crushed culture medium raw materials into a culture medium container, and sealing the culture medium container;
and S3, sterilizing the culture medium container filled with the culture medium raw material by high-pressure steam, placing the culture medium container into a high-pressure steam sterilizing device for sterilizing, and inactivating the microorganisms in the culture medium raw material.
Referring to fig. 2-10, the culture medium container includes a wide-mouth bottle 1, the wide-mouth bottle 1 is filled with a culture medium for edible fungi, a connection sleeve 2 matched with the wide-mouth bottle 1 is inserted into an opening at the upper end of the wide-mouth bottle 1, the connection sleeve 2 includes a fixing portion 201, an inclined plane groove 202 is cut at the upper end of the fixing portion 201, a steam through hole 203 is fixedly cut at the bottom of the inclined plane groove 202, the steam through hole 203 penetrates through the fixing portion 201, a sealing cover 4 matched with the steam through hole 203 is inserted into the steam through hole 203, the sealing cover 4 includes a cover body 401, a steam channel 402 is cut in the cover body 401, the steam channel 402 communicates the wide-mouth bottle 1 with the outside, a support net 406 matched with the steam channel 402 is fixedly connected to an opening at the upper end of the steam channel 402, a waterproof curtain 407 is arranged at the upper end of the support net 406, the support net 406 is fixedly connected to a central portion of the waterproof curtain 407, a plurality of tearing grooves 408 are cut on the waterproof curtain 407, the plurality of tearing grooves 408 cut the edge portion of the waterproof curtain 407 into a plurality of sectors, the lower end of the fixing portion 201 is fixedly connected with a soil loosening device 5, the soil loosening device 5 comprises a power source 501, the power source 501 is fixed at the lower end of the fixing portion 201, a soil loosening unit matched with the power output end of the power source 501 is fixedly connected with a power output end of the power source 501, the whole length of the soil loosening device 5 can be prolonged by connecting two adjacent soil loosening units end to end, the soil loosening unit comprises a connecting rod 502, one end, close to the power output end of the power source 501, of the connecting rod 502 is provided with a mounting groove 503 matched with the power output end of the power source 501 in a chiseled mode, one end, far away from the mounting groove 503, of the connecting rod 502 is provided with a positioning table 504 matched with the mounting groove 503 in a chiseled mode, the outer side of the positioning table 504 is clamped with a positioning ring 505 matched with the positioning table 504, the inner wall of the positioning ring 505 is provided with a plurality of fan blades 507, and a fixing cover 508 is fixedly connected with the positioning table 504 of the soil loosening unit at the lowest end, can realize in the culture medium sterilization process, choose wide mouthful container for holding the culture medium for use, the bottling and the taking out of the culture medium of being convenient for increase culture medium sterilization efficiency.
Be connected with seal ring 3 between wide-mouthed bottle 1 and the adapter sleeve 2, seal ring 3 and wide-mouthed bottle 1 fixed connection increase the sealed effect between wide-mouthed bottle 1 and the adapter sleeve 2 for in external high-pressure steam is difficult for entering into wide-mouthed bottle 1 through the clearance between wide-mouthed bottle 1 and the adapter sleeve 2, be difficult for influencing the culture medium that loads in the wide-mouthed bottle 1.
Be equipped with sealed gluey piece 403 between lid 401 and adapter sleeve 2, sealed gluey piece 403 and lid 401 fixed connection, sealed gluey piece 403 can adapt to inclined plane groove 202, be difficult for influencing the leakproofness between adapter sleeve 2 and the sealed lid 4 after 4 installation dismantles of sealed lid, it has the elasticity cavity to open in the sealed gluey piece 403, the intussuseption of elasticity cavity is filled with a plurality of packing balls 404, increase the elasticity of sealed gluey piece 403, increase the adaptability of sealed gluey piece 403, increase the sealed effect between adapter sleeve 2 and the sealed lid 4, equal fixedly connected with elastic fiber 405 on the outer wall of a plurality of packing balls 404, adjacent elastic fiber 405 twines together, increase the bonding strength between the adjacent packing ball 404, make sealed gluey piece 403 be difficult for taking place excessive deformation under the exogenic action, be difficult for influencing the sealed effect of sealed gluey piece 403.
The inner wall of the tearing groove 408 is fixedly connected with waterproof fibers 409, adjacent waterproof fibers 409 are wound together to form a three-dimensional space structure, on the premise that the movement of the waterproof curtain 407 is not influenced, the integral sealing effect of the waterproof curtain 407 is increased, so that external water vapor is not easy to enter the wide-mouth bottle 1 through the sealing cover 4, a plurality of waterproof fibers 409 are all in a three-dimensional spiral shape, the stability of the three-dimensional space structure formed by the plurality of waterproof fibers 409 is increased, the waterproof effect is increased, the hydrophobic coating is coated on the outer walls of the plurality of waterproof fibers 409, in the cooling process after the sterilization of the culture medium is completed, water formed by condensation is easy to roll off on the surfaces of the waterproof curtain 407 and the waterproof fibers 409, the components of the culture medium are not easy to affect in the wide-mouth bottle 1, the outer edge of the upper end of the cover body 401 is in a form with a high center and a low outer part, so that water drops rolled off on the waterproof curtain 407 can directly slide off from the upper end of the cover body 401, is not easy to be accumulated in a large amount.
One end of each positioning plate 506 close to the inner wall of the positioning ring 505 is provided with a plurality of hairline cracks, and the strength of the positioning plate 506 can be controlled by controlling the number and the depth of the hairline cracks, so that when the fan blades 507 cannot rotate under the action of external force, the positioning plates 506 can automatically break, the connection between the connecting rods 502 and the fan blades 507 in the soil loosening unit is disconnected, and the power source 501 is not easy to be burnt due to short circuit caused by idling.
According to the scheme, firstly, the culture medium raw material is coarsely ground to meet the requirements of subsequent processes, the granularity of the coarsely ground culture medium raw material is determined by the specific size and specification of the culture medium container, which is a well-known technology of a person skilled in the art and is not disclosed in detail, then the coarsely ground culture medium raw material is added into the wide-mouth bottle 1, filling of the culture medium raw material can be greatly simplified due to the chiseling of the inclined plane groove 202, after filling is finished, the connecting sleeve 2 is sealed by the sealing cover 4 to complete filling of the raw material, and then the culture medium container filled with the culture medium raw material is placed into a high-pressure steam sterilization device for sterilization.
Atmospheric pressure in the in-process wide-mouthed bottle 1 internal and external environment that carries out high pressure steam sterilization all can rise, because culture medium raw materials can produce certain gas under the high temperature effect, this limit can lead to the inside atmospheric pressure rise range of wide-mouthed bottle 1 to be greater than external, can jack-up the border position of waterproof curtain 407 this moment, form the space, release the inside high-pressure gas of wide-mouthed bottle 1, and in the above-mentioned in-process, because the air current direction is outside in being from wide-mouthed bottle 1, consequently can not cause external a large amount of steam to enter into wide-mouthed bottle 1 in the pollution culture medium raw materials, and atmospheric pressure in wide-mouthed bottle 1 resumes when being the same with the external world, waterproof curtain 407 resumes the original shape, external steam is difficult for entering into wide-mouthed bottle 1, form the protection once more.
Meanwhile, the scarification device is arranged on the connecting sleeve 2, so that the raw materials of the culture medium can be loosened to be relatively fluffy, on one hand, the heat conduction is convenient, the sterilization effect is improved, on the other hand, the subsequent cultivation of edible fungi is also convenient, as for the specific control method of the scarification device, the method is the normal use method of the high-temperature resistant electric appliance, and the method can be obtained by the prior art and is not disclosed in detail in the application, in particular, the soil loosening device in the scheme is formed by splicing a plurality of soil loosening units, is convenient for subsequent maintenance and repair, meanwhile, the strength of the positioning plate 506 is controlled by controlling the number and the depth of the hairline cracks of the soil loosening unit, when the fan blade 507 is unable to rotate under the action of external force, the positioning plate 506 can break automatically to break the connection between the connecting rod 502 and the fan blade 507 in the soil loosening unit, so that the power source 501 is not easy to be burnt out due to short circuit caused by idle running.
The foregoing is only a preferred embodiment of the present invention; the scope of the invention is not limited thereto. Any person skilled in the art should be able to cover the technical scope of the present invention by equivalent or modified solutions and modifications within the technical scope of the present invention.

Claims (10)

1. A sterilization method for an edible fungus culture medium is characterized by comprising the following steps: the method mainly comprises the following steps:
s1, coarse grinding of the raw materials of the culture medium, and breaking of lumps in the raw materials of the culture medium;
s2, filling raw materials, namely filling the coarsely crushed culture medium raw materials into a culture medium container, and sealing the culture medium container;
and S3, sterilizing the culture medium container filled with the culture medium raw material by high pressure steam, and putting the culture medium container into a high pressure steam sterilization device for sterilization to inactivate the microorganisms in the culture medium raw material.
2. The method for sterilizing the edible fungus culture medium according to claim 1, wherein the method comprises the following steps: the culture medium container comprises a wide-mouth bottle (1), an edible fungus culture medium is filled in the wide-mouth bottle (1), a connecting sleeve (2) matched with the wide-mouth bottle is inserted into an upper end opening of the wide-mouth bottle (1), the connecting sleeve (2) comprises a fixing part (201), an inclined plane groove (202) is formed in the upper end of the fixing part (201), a steam through hole (203) is fixedly formed in the bottom of the inclined plane groove (202), the steam through hole (203) penetrates through the fixing part (201), a sealing cover (4) matched with the wide-mouth bottle is inserted into the steam through hole (203), the sealing cover (4) comprises a cover body (401), a steam channel (402) is formed in the cover body (401), the steam channel (402) is communicated with the wide-mouth bottle (1) and the outside, a supporting net (406) matched with the wide-mouth bottle is fixedly connected to an opening at the upper end of the steam channel (402), the upper end of the supporting net (406) is provided with a waterproof curtain (407), the supporting net (406) is fixedly connected with the center of the waterproof curtain (407), the waterproof curtain (407) is provided with a plurality of tearing grooves (408), the tearing grooves (408) cut the edge of the waterproof curtain (407) into a plurality of fan shapes, the lower end of the fixing part (201) is fixedly connected with a soil loosening device (5), the soil loosening device (5) comprises a power source (501), the power source (501) is fixed at the lower end of the fixing part (201), the power output end of the power source (501) is fixedly connected with soil loosening units matched with the power source (501), every two soil loosening units are connected end to end, each soil loosening unit comprises a connecting rod (502), one end of the connecting rod (502) close to the power output end of the power source (501) is provided with a mounting groove (503) matched with the power output end of the power source (501), the one end of keeping away from mounting groove (503) of connecting rod (502) is opened and is chiseled and have and mounting groove (503) assorted location platform (504), the outside joint of location platform (504) has and self assorted holding ring (505), open on the inner wall of holding ring (505) and chisel and have and fix a position platform (504) assorted locating plate (506), a plurality of fan blades of fixedly connected with (507) are located the lower extreme on the outer wall of holding ring (505) fixedly connected with fixed cover (508) on location platform (504) of the unit that loosens the soil.
3. The method for sterilizing the culture medium of the edible fungi according to claim 2, wherein the method comprises the following steps: be connected with seal ring (3) between wide-mouthed bottle (1) and adapter sleeve (2), seal ring (3) and wide-mouthed bottle (1) fixed connection.
4. The method for sterilizing the culture medium of the edible fungi according to claim 2, wherein the method comprises the following steps: be equipped with between lid (401) and adapter sleeve (2) sealant piece (403), sealant piece (403) and lid (401) fixed connection.
5. The method for sterilizing the culture medium of the edible fungi according to claim 4, wherein the method comprises the following steps: an elastic cavity is chiseled in the sealing rubber block (403), and a plurality of filling balls (404) are filled in the elastic cavity.
6. The method for sterilizing the culture medium of the edible fungi according to claim 5, wherein the method comprises the following steps: elastic fibers (405) are fixedly connected to the outer walls of the filling balls (404), and the adjacent elastic fibers (405) are wound together.
7. The method for sterilizing the culture medium of the edible fungi according to claim 2, wherein the method comprises the following steps: the inner wall of the tearing groove (408) is fixedly connected with waterproof fibers (409), and the adjacent waterproof fibers (409) are wound together to form a three-dimensional space structure.
8. The method for sterilizing the culture medium of the edible fungi according to claim 7, wherein the method comprises the following steps: the waterproof fibers (409) are in a three-dimensional spiral shape, and hydrophobic paint is coated on the outer walls of the waterproof fibers (409).
9. The method for sterilizing the culture medium of the edible fungi according to claim 2, wherein the method comprises the following steps: the outer edge of the upper end of the cover body (401) is in a shape that the center is high and the outer part is low.
10. The method for sterilizing the culture medium of the edible fungi according to claim 2, wherein the method comprises the following steps: and a plurality of capillary cracks are drilled at one ends of the positioning plates (506) close to the inner wall of the positioning ring (505).
CN202210365118.9A 2022-04-08 2022-04-08 Sterilization method for edible fungus culture medium Pending CN114747429A (en)

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