CN114717290A - Nucleic acid detection card box capable of preventing nucleic acid pollution - Google Patents

Abstract

The invention belongs to the field of nucleic acid detection, and discloses a nucleic acid detection card box capable of preventing nucleic acid pollution, which comprises a closed bottom plate, wherein the bottom plate is provided with: the chip socket is used for inserting and detecting a chip with a detection sample; the chip destruction structure is used for destroying the sealing film on the chip and releasing the detection sample; the liquid storage bubble cap is used for prestoring buffer liquid; the bubble cap releasing module is used for destroying the liquid storage bubble cap and releasing the buffer solution; and the test strip, the detection sample and the buffer solution flow into the test strip after being uniformly mixed. The nucleic acid detection card box breaks the seal of a chip sealing membrane through a chip breaking structure and releases a detection sample, the buffer solution is released through a pressure storage liquid bubble cover, the detection sample diluted by the buffer solution automatically flows to a sample loading area of a test strip, and then the detection and analysis of a nucleic acid amplification product are realized on the test strip through a chromatography and an immune combination principle.

Description

Nucleic acid detection card box capable of preventing nucleic acid pollution

Technical Field

The invention relates to the field of nucleic acid detection, in particular to a universal nucleic acid detection card box capable of preventing nucleic acid pollution.

Background

The nucleic acid detection is one of the main detection modes in the field of in vitro diagnosis, is a detection method with higher accuracy, can quickly detect a specific nucleic acid sequence in a biological sample of a patient by utilizing the technologies such as a PCR (polymerase chain reaction) technology, a nucleic acid sequencing technology, molecular hybridization and the like, provides medical test evidence for the diagnosis of an infected case, wins the optimal treatment time for an infected person, and reduces the virus death rate. Has been widely used in the research of infectious disease diagnosis, epidemic disease investigation, food hygiene inspection, early diagnosis of tumor and genetic disease, forensic identification and other fields.

Polymerase Chain Reaction (PCR) is an exponential amplification of a DNA segment to be amplified to a sufficient amount for structural and functional analysis by taking a DNA molecule to be amplified as a template, taking a pair of oligonucleotide fragments complementary to the 5 'end and the 3' end of the template, respectively, as primers, and participating with both DNA Polymerase and nucleotide substrates according to a mechanism of half-retention replication. The PCR detection method has extremely important significance in clinically and rapidly detecting pathogens and the like.

The PCR technology can amplify target DNA millions of times in a short time, shows extremely high sensitivity, can detect viruses which are difficult to detect by common inspection, has the advantages of high specificity, rapidness, low requirement on samples and the like, and is widely applied to clinical diagnosis in hospitals and diagnosis of poultry diseases of various epidemic prevention detection departments. However, due to its extremely high sensitivity and the detection method (agarose gel electrophoresis) of the amplification product uncapping, DNA contamination is very easily caused, and a very small amount of contamination can cause a false positive result, if aerosol contamination is formed and diffused, the entire laboratory can be contaminated, the treatment is very troublesome, and even the laboratory needs to be shut down. Therefore, it is particularly important to avoid nucleic acid contamination and to eliminate false positive results.

The commonly used methods for preventing nucleic acid aerosol pollution at present are as follows: 1. carrying out experiments in a standard PCR laboratory, wherein the PCR laboratory is divided into a preparation area, an amplification area and an analysis area, and the areas are mutually separated and independently exhausted; 2. performing analysis of PCR amplification products in a fume hood or a specific independent space; 3. spraying a special DNA pollution scavenger to operating environments such as a laboratory bench and the like; 4. introducing an anti-pollution enzyme, such as UNG-dUTP system, into the PCR reaction system to degrade the pollution in the reaction system; 5. the real-time fluorescence PCR is used for carrying out the totally enclosed reaction, the amplification process is detected in real time, and the post-treatment of the product is not needed. These methods currently have limitations: the standard PCR laboratory requires enough space for partition setting in the laboratory, and the decoration cost of the PCR laboratory is high. Fume hoods can reduce aerosol contamination, but cannot completely avoid or eliminate nucleic acid contamination. Other methods are not effective in removing aerosol contamination over a large area, and the price of DNA scavengers and anti-contamination reagents is also relatively high. Real-time fluorescent PCR is not suitable for use in substrates because of the need for expensive laboratory equipment and reagents and the manipulation of specialized personnel.

Disclosure of Invention

The invention aims to at least solve one of the problems of the existing nucleic acid detection device, therefore, the invention provides a nucleic acid pollution-proof nucleic acid detection card box and a chip with a detection sample for matching detection, which are suitable for end point analysis of products of nucleic acid detection, have the advantages of totally closed and pollution-free use process, high reliability, low preparation cost and high fault tolerance rate.

Specifically, the invention adopts the following technical scheme:

a nucleic acid detecting cartridge which is protected from nucleic acid contamination, comprising a closed bottom plate on which are provided:

the chip socket is used for inserting a chip packaged with a detection sample;

the chip destruction structure is used for destroying the sealing film on the chip and releasing the detection sample;

the liquid storage bubble cap is used for prestoring buffer liquid;

the bubble cap releasing module is used for destroying the liquid storage bubble cap and releasing the buffer solution;

and the test strip, the detection sample and the buffer solution flow into the test strip after being uniformly mixed.

Further, the cross-sectional area of the chip is larger than that of the chip socket, and the chip socket and the inserted chip form interference fit.

Further, the chip socket is provided with a flexible sealing sleeve, and the chip socket and the inserted chip form interference fit.

Furthermore, the chip comprises a sample adding opening, a reaction area, a sealing film and a sealing film, wherein the sealing film is used for sealing the sample adding opening, and the sealing film is used for sealing the reaction area.

Furthermore, a sample adding port slide way is arranged at the sample adding port of the chip, and a reaction area slide way is arranged at the reaction area.

Further, the chip destruction structure includes a first bump structure and a second bump structure.

Further, the number of the first projection structures is one or more.

Further, the number of the second projection structures is one or more.

Furthermore, first protruding structure is used for destroying the sealing membrane, first protruding structure with the sample injection port slide forms interference fit.

Further, the second protruding structure is used for destroying the sealing film, and the second protruding structure and the reaction area slide form interference fit.

Furthermore, a difference of 0.05-0.1mm exists between the height of the first protrusion structure and the depth of the slide way of the sample port slide way.

Further, there is a difference of 0.05-0.1mm between the height of the protrusions of the second protrusion structures and the depth of the ramps of the reaction zone ramps.

Further, the bottom surface of the liquid storage bubble cap is connected with the bottom plate in an adhesive mode.

Further, the bottom surface of the liquid storage bubble cap is made of plastic or aluminum thin film materials.

Further, the top surface of the liquid storage bubble cap is provided with a flexible sealing layer.

Further, the blister release module comprises a buffer release port, a buffer release pool, a convex breaking part and a blister fixing surface.

Furthermore, a sample buffer chamber and a test strip sample pool which are communicated are also arranged on the bottom plate, and the sample pad end of the test strip is arranged in the test strip sample pool.

Furthermore, the bottom plate is also provided with a first air chamber and a second air chamber, the chip destruction structure is arranged in the first air chamber, and the absorption pad end of the test strip is arranged in the second air chamber.

Further, the bottom plate includes first bottom plate and second bottom plate, the chip socket is located between first bottom plate and the second bottom plate, bubble cap release module, first protruding structure and first air chamber set up in on the first bottom plate, second protruding structure, second air chamber, sample buffer chamber, test paper strip sample cell and test paper strip set up in on the second bottom plate.

The invention has the following technical effects:

(1) in the nucleic acid pollution-preventing nucleic acid detection card box, the nucleic acid detection test strip is used for analyzing the amplification product, and the test strip is assembled in the closed card box to prevent the pollution of the nucleic acid caused by opening the cover.

(2) In the nucleic acid detection card box for preventing the nucleic acid pollution, a nucleic acid detection chip with an easily-damaged sealing membrane is used as a detection sample carrier, and a matched chip damage structure is arranged in the card box to damage the sealing of the chip sealing membrane and release a detection sample (a nucleic acid amplification product). Meanwhile, a buffer solution required by dilution of a detection sample is prestored in the liquid storage bubble cap, the buffer solution released by the liquid storage bubble cap is manually pressed after the detection sample is released, the detection sample diluted by the buffer solution automatically flows to a sample loading area of the test strip, and then detection and analysis of a nucleic acid amplification product are realized on the test strip through a chromatography effect and an immune combination principle.

Drawings

FIG. 1 shows a schematic view of a nucleic acid detecting cartridge which is protected from nucleic acid contamination;

FIG. 2 shows a schematic diagram of a chip structure;

FIG. 3 is a schematic view showing the use of a nucleic acid detecting cartridge for preventing nucleic acid contamination in combination with a chip;

FIG. 4 shows a schematic structural view of a blister release module;

FIG. 5 shows a schematic structural diagram of a test strip;

FIG. 6 is a schematic diagram showing a chip destruction structure;

FIG. 7 shows a schematic view of the first base plate;

fig. 8 shows a schematic structural view of the second base plate.

In the figure: 1-chip socket; 2-chip destruction structure, 21-first bump structure, 22-second bump structure, first bump 211, air channel 212, second bump 221, flow channel 222; 3-liquid storage bubble cap; 4-a bubble cap release module, 41-a buffer solution release port, 42-a bulge destroying part, 43-a bubble cap fixing surface and 44-a buffer solution release pool; 5-test strip, 51-sample pad, 52-conjugate pad, 53-detection zone, 54-absorption pad; 6-a sample buffer chamber; 7-a test strip sample cell; 8-test strip fixing surface; 9-detection line identification; 10-a first air chamber; 11-a second air chamber;

100-chip, 101-sample port, 102-reaction area, 103-sample port slide way, 104-reaction area slide way;

the first bottom plate A, the second bottom plate B and the cover plate C.

Detailed Description

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. All patents and publications referred to herein are incorporated by reference in their entirety. The term "comprising" or "comprises" is open-ended, i.e. comprising what is specified in the present invention, but not excluding other aspects.

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

Referring to FIGS. 1 to 8, the nucleic acid contamination prevention nucleic acid detecting cartridge includes a closed bottom plate on which: the test strip comprises a chip socket 1, a chip destruction structure 2, a liquid storage bubble cap 3, a bubble cap release module 4, a test strip 5, a sample buffer chamber 6 and a test strip sample cell 7.

Referring to FIG. 2, the nucleic acid contamination prevention nucleic acid detecting cartridge is used for detecting a chip 100 with a detection sample, the chip 100 includes a sample addition port 101, a reaction region 102, a sealing film (not shown) for sealing the sample addition port 101, and a sealing film (not shown) for sealing the reaction region 102. Before use, a test sample is introduced from the sample inlet 101 to the reaction region 102, and then the sample inlet 101 is sealed with a sealing film to form a sealed chip 100. After the chip 100 is inserted into the nucleic acid detection card box for preventing nucleic acid pollution, the chip destruction structure 2 destroys the sealing film on the chip 100 to release a detection sample, the bubble cap release module 4 destroys the liquid storage bubble cap 3 to release buffer solution, the detection sample and the buffer solution are mixed uniformly and then flow into the test strip 5, and a detection result is displayed.

Form interference fit between the inserted chip and the chip socket and realize totally enclosed detection, after the chip 100 is inserted into the card box, sample release, buffer solution release and product detection operation are all carried out in airtight environment, prevent nucleic acid pollution, can ensure the safety problem of testing personnel in the use. In some embodiments, the cross-sectional area of the chip is greater than the cross-sectional area of the chip socket such that an interference fit is formed between the inserted chip and the chip socket. In some embodiments, the chip socket is provided with a flexible sealing sleeve, so that an interference fit is formed between the inserted chip and the chip socket, the elasticity of the flexible sealing sleeve at the chip socket is mainly utilized to form a fit with the inserted hard chip, the sealing effect between the chip and the card box is ensured, meanwhile, the sealing effect of the sealing mode is good, and the requirement on the processing precision of the card box and the chip is obviously reduced.

The chip destruction structure 2 is a protruding structure, and the protruding structure forms an interference fit with the chip 100. Therefore, the sealing film and the sealing film can be better damaged by interference fit, and the sample release effect is ensured.

Referring to fig. 1 to fig. 3, a sample port slide 103 is disposed at a sample port 101 of the chip, a reaction area slide 104 is disposed at a reaction area 102, the chip destruction structure 2 includes a first protrusion structure 21 and a second protrusion structure 22, the first protrusion structure 21 is used for destroying a sealing film, and forms an interference fit with the sample port slide 103; the second raised structure 22 is used to break the sealing film and form an interference fit with the reaction zone slide 104. A difference of 0.05-0.1mm exists between the projection height of the first projection structure 21 and the slide depth of the sample port slide 103; there is a difference of 0.05-0.1mm between the protrusion height of the second protrusion structure 22 and the slide depth of the reaction zone slide 104 in order to form an interference fit. During insertion of the chip into the cartridge, the first projection structure 21 moves along the sample port slide 103, and the second projection structure 22 moves along the reaction area slide 104; after the chip is completely inserted into the back card box, the first protruding structure 21 moves to the sealing film and punctures the sealing film, the second protruding structure 22 moves to the sealing film and punctures the sealing film, at this time, the first protruding structure 21 forms interference fit with the sample injection port slide way 103, and the second protruding structure 22 forms interference fit with the reaction area slide way 104. After the sealing film of the chip 100 is broken, the test sample is released into the sample buffer chamber 6.

The required buffer solution of test paper strip 5 sample loading is prestored in stock solution bubble cap 3, and the bottom surface and the bottom plate adhesive connection of stock solution bubble cap 3, for example, connect the bottom surface of stock solution bubble cap 3 on the bottom plate through the double faced adhesive tape. The bottom surface of the liquid storage bubble cap 3 is made of a breakable material, such as a plastic or aluminum film material, and the top surface of the liquid storage bubble cap 3 is a flexible sealing layer. Thus, the buffer solution is released by squeezing the flexible sealing layer of the top surface of the reservoir blister 3 so that the bottom surface of the reservoir blister 3 is adjacent to the blister release module 4 and is destroyed.

Referring to fig. 4, the blister release module 4 includes a buffer release port 41, a convex breaking portion 42, a blister fixing surface 43, and a buffer release reservoir 44. Stock solution bubble cap 3 is fixed in the bottom plate in bubble cap stationary plane 43 department, and when extrusion stock solution bubble cap 3, the easy destruction material of 3 bottoms surfaces of stock solution bubble cap is destroyed to protruding destruction portion 42, and is further, protruding destruction portion 42 is the arch that has the acute angle structure, bubble cap stationary plane 43's shape is the annular. After the bubble cap 3 is destroyed, the buffer solution flows out of the buffer solution release port 41 and then flows into the sample buffer chamber 6 through the buffer solution release reservoir 44. The detection sample and the buffer solution are uniformly mixed in the sample buffer chamber 6, and the uniformly mixed detection sample mixed solution flows into the test strip sample cell 7.

The total volume of the buffer solution prestored in the liquid storage bubble cap 3 is 50-150 mu L. In some embodiments, the total volume of pre-stored buffer is 70-130. mu.L. In some embodiments, the total volume of pre-stored buffer is 90-110. mu.L.

The buffer volume of the sample buffer chamber 6 is 10-50 muL. In some embodiments, the buffer volume of the sample buffer chamber 6 is 20-40 μ L. In some embodiments, the buffer volume of the sample buffer chamber 6 is 25-35 μ L.

Referring to fig. 5, the test strip 5 includes a sample pad 51, a conjugate pad 52, a detection zone 53 and an absorbent pad 54, wherein the detection zone 53 includes a plurality of color development bands 531 (detection lines). Wherein the sample pad 51 is placed in the test strip sample cell 7, and the test sample mixed solution flows into the test strip 5 through the sample pad 51.

In some embodiments, the detection zone 53 is provided with two detection lines and one chromatography quality control line. In some embodiments, the detection zone 53 is provided with three detection lines and one chromatography quality control line. In some embodiments, where sample amplification is otherwise quality controlled, the detection lines at the detection zone 53 are used for target nucleic acid detection.

The bottom plate is also provided with a test strip fixing surface 8, and the test strip 5 is fixed on the bottom plate in an adhesive manner at the test strip fixing surface 8. The bottom plate is also provided with a detection line mark 9 for observing the color development condition of the detection line of the test strip detection area 53 so as to judge the detection result.

The bottom plate is further provided with a first air chamber 10, the first protruding structure 21 is arranged in the first air chamber 10, the chip socket 1 is communicated with the first air chamber 10, and after the chip 100 is inserted, the first protruding structure 21 breaks the sealing film, so that the chip sample adding port 101 is communicated with the inside of the first air chamber 10.

The bottom plate is further provided with a second air chamber 11, the end of the absorption pad 54 of the test strip 5 is arranged in the second air chamber 11, and the chip socket 1 is communicated with the second air chamber 11.

The first and second air chambers 10 and 11 may allow air pressure to equalize between the chip 100 and the cartridge, facilitating the flow of test samples between the chip and the cartridge and within the cartridge.

Referring to fig. 6, the first bump structure 21 includes a first bump 211 and an air channel 212, and the air channel 212 is located in a middle region of the first bump 211 and helps to communicate gas between the chip loading port 101 and the first air chamber 10 after the sealing film is broken by the first bump structure 21.

The second bump structure 22 includes a second bump 221 and a flow channel 222, the flow channel 222 is located in the middle region of the second bump 221, and helps to communicate the fluid between the chip reaction region 102 and the sample buffer chamber 6 after the second bump structure 22 breaks the sealing film.

With continued reference to fig. 1 to 8, the base plate includes a first base plate a and a second base plate B, the chip socket 1 is located between the first base plate a and the second base plate B, the liquid storage bubble cap 3, the bubble cap releasing module 4, the first protruding structure 21 and the first air chamber 10 are disposed on the first base plate a, and the second protruding structure 22, the second air chamber 11, the sample buffer chamber 6, the test strip sample cell 7, the test strip 5 and the test strip identifier 9 are disposed on the second base plate B.

Further, the liquid storage bubble cap 3 is positioned on the side of the first base plate A away from the second base plate B, which is also called the back of the first base plate A, so that the liquid storage bubble cap 3 is pressed when in use; the buffer release port 41 of the blister release module 4 penetrates the first bottom plate a to facilitate the outflow of the buffer. The sample buffer chamber 6 and the strip well 7 are located on the side of the second base plate B facing the first base plate a, also referred to as the back side of the second base plate B, to facilitate the inflow of the test sample and the buffer. The test strip 5 and the detection line mark 9 are positioned on the side of the second bottom plate B away from the first bottom plate a, also called the front side of the second bottom plate B, so as to facilitate the observation of the detection result. The second air chamber 11 and the strip reservoir 7 extend through the second base plate B.

The bottom plate further comprises a cover plate C, and the cover plate C is used for sealing one side, far away from the first bottom plate A, of the second bottom plate B. Namely, a first bottom plate A, a second bottom plate B and a cover plate C are sequentially attached to prepare the nucleic acid detection card box for preventing nucleic acid pollution, and the cover plate surface is called as the front surface.

The base plate can be made of common engineering plastics, for example, the first base plate a, the second base plate B and the cover plate C can be made of any one of PMMA, PC and PS.

The preparation of the nucleic acid pollution prevention nucleic acid detection card box comprises the following steps: respectively manufacturing a first bottom plate A, a second bottom plate B and a cover plate C by common processing means such as a machine tool, injection molding and the like; common sealing means such as hot pressing, laser, ultrasound and the like are used between the first bottom plate A and the second bottom plate B and between the second bottom plate B and the cover plate C to realize sealing; the liquid storage bubble cap 3 is fixedly connected to one side, far away from the second bottom plate B, of the first bottom plate A by gluing, mechanical fastening and the like; the test paper strip 5 is fixedly connected to one side of the second bottom plate B far away from the first bottom plate A in a gluing mode and the like.

The use process of the nucleic acid detection card box for preventing the nucleic acid pollution comprises the following steps:

s1: sample release: inserting a chip containing a detection sample into a chip socket, destroying a sealing film on the chip by using a chip destruction structure, and releasing the detection sample to a sample buffer chamber;

s2: buffer release: pressing the liquid storage bubble cap, destroying the bottom surface of the liquid storage bubble cap by using a bubble cap releasing module, releasing a buffer solution to the sample buffer chamber, and shaking the card box to uniformly mix the detection sample and the buffer solution;

s3: and (3) test strip detection: the mixed liquid of the detection sample after being mixed evenly flows into a test strip sample pool, soaks a test strip sample pad placed in the test strip sample pool, and starts a chromatography detection process;

s4: and (3) detection results: judging and reading the detection result according to the color development condition of the test strip detection line, wherein a color development strip indicates that the sample contains the target nucleic acid with the concentration exceeding the detection limit, a color development-free strip indicates that the sample does not contain the target nucleic acid or the target nucleic acid does not reach the detection limit, and a chromatographic quality control line indicates that the detection is invalid;

s5: and (3) post-treatment: after the detection is finished, the card box inserted with the chip is recycled to the biological garbage can as a whole, and finally, the unified destruction treatment is carried out.

Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention.

Example 1

The parameters of the present embodiment are as follows: the plastic packaging structure comprises a first bottom plate with the thickness of 4mm, a second bottom plate with the thickness of 3mm and a cover plate with the thickness of 1mm, wherein the first bottom plate, the second bottom plate and the cover plate are all made of PMMA and are finished by injection molding; soaking and washing the processed first base plate, second base plate and cover plate with clear water for 30min, ultrasonic bathing with 100mM NaOH and 0.1% Sodium Dodecyl Sulfate (SDS) for 30min, and washing with deionized water; the first base plate and the second base plate which are processed are covered, a flexible sealing sleeve is filled in a chip socket, the two base plates are sealed through ultrasonic welding, then the test paper strip is fixed on the test paper strip fixing surface of the second base plate through double faced adhesive tape, the cover plate is sealed on one side, far away from the first base plate, of the second base plate through ultrasonic welding, the liquid storage bubble cap pre-stored with 100 mu L buffer solution is fixed on the first base plate through the double faced adhesive tape to form a complete card box, and the card box is packaged with an aluminum foil tape before use.

The test strip comprises three detection lines which are respectively corresponding to three different antibody modifications of DIG, FITC and TARMA, and the public colloidal gold is modified by BIO antibody, so that the amplification primers used by the nucleic acid sample to be detected comprise BIO-DIG, BIO-FITC and BIO-TARMA modifications; in addition, the volume buffer volume of the sample buffer was 30. mu.L.

Detection of nucleic acid amplification products using the cartridge of the invention:

firstly, respectively amplifying an S gene positive template and pure water in two nucleic acid detection chips, using a 50 mu L LAMP direct amplification reagent and a BIO-DIG modified S gene LMAP primer, amplifying for 60min at 65 ℃, and taking the amplified product as a test sample. Take out the card box of aluminium foil encapsulation, tear the aluminium foil encapsulation and take out the card box and vertically place according to the direction that the socket faces up, then insert the chip that contains the nucleic acid amplification product that awaits measuring into the card box socket and carry out nucleic acid testing:

s1: sample release: inserting a chip containing a nucleic acid amplification product to be detected into a chip socket, and using a chip destruction structure to destroy an encapsulation film on the chip and release a detection sample to a sample buffer chamber;

s2: buffer release: pressing the liquid storage bubble cap, destroying the bottom surface of the liquid storage bubble cap by using a bubble cap releasing module, releasing a buffer solution to the sample buffer chamber, and shaking the card box to uniformly mix the detection sample and the buffer solution;

s3: and (3) detecting the test strip: the mixed liquid of the detection samples after being mixed evenly flows into a test strip sample pool, soaks a test strip sample pad placed in the test strip sample pool, starts a chromatography detection process, and judges the result after waiting for 3-5min or an absorption pad is completely soaked and a chromatography quality control line is striped;

s4: and (3) detection results: developing bands appear on a DIG detection line and a chromatography quality control line on a cartridge test strip detected by a positive template, and only the chromatography quality control line appears on the cartridge test strip detected by a pure water sample;

s5: and (3) post-treatment: after the detection is finished, the card box inserted with the chip is taken as a whole to be recycled to the biological garbage can, and finally, the unified destruction treatment is carried out.

In the description herein, references to the description of the terms "some embodiments," "other embodiments," "an embodiment," "an example," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.

Although embodiments of the present invention and examples have been shown and described above, it is understood that the above embodiments, examples are illustrative and not to be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments, examples by those of ordinary skill in the art within the scope of the present invention.

Claims (10)

1. A nucleic acid pollution prevention nucleic acid detection card box is characterized by comprising a closed bottom plate, wherein the bottom plate is provided with:

the chip socket is used for inserting a chip packaged with a detection sample;

the chip destruction structure is used for destroying the sealing film on the chip and releasing the detection sample;

the liquid storage bubble cap is used for prestoring buffer liquid;

the bubble cap releasing module is used for destroying the liquid storage bubble cap and releasing the buffer solution;

and the test strip, the detection sample and the buffer solution flow into the test strip after being uniformly mixed.

2. The nucleic acid contamination-preventing nucleic acid detecting cartridge according to claim 1, wherein a cross-sectional area of the chip is larger than a cross-sectional area of a chip insertion port which forms an interference fit with an inserted chip; or the chip socket is provided with a flexible sealing sleeve, and the chip socket and the inserted chip form interference fit.

3. The nucleic acid contamination-preventing nucleic acid detecting cartridge according to claim 1, wherein the chip comprises a sample addition port, a reaction region, a sealing film for sealing the sample addition port, and a sealing film for sealing the reaction region.

4. The nucleic acid contamination prevention nucleic acid detecting cartridge according to claim 3, wherein the sample application port of the chip has a sample application port slide, the reaction region has a reaction region slide, the chip destruction structure includes a first protruding structure and a second protruding structure, the first protruding structure is used for destroying the sealing film, the first protruding structure forms an interference fit with the sample application port slide, the second protruding structure is used for destroying the sealing film, and the second protruding structure forms an interference fit with the reaction region slide.

5. The nucleic acid contamination resistant nucleic acid detection cartridge of claim 4, wherein there is a difference of 0.05-0.1mm between the height of the projections of the first projection structure and the depth of the slide of the sample addition port slide; there is a difference of 0.05-0.1mm between the height of the protrusions of the second protrusion structures and the depth of the slide of the reaction zone slide.

6. The nucleic acid contamination-proof nucleic acid detecting cartridge according to claim 1, wherein the bottom surface of the liquid storage blister is adhesively connected with the bottom plate, the bottom surface of the liquid storage blister is made of plastic or aluminum film material, and the top surface of the liquid storage blister is provided with a flexible sealing layer.

7. The nucleic acid contamination-preventive nucleic acid detecting cartridge according to claim 1, wherein the blister release module comprises a buffer release port, a buffer release well, a bulge destroying portion and a blister fixing face.

8. The nucleic acid contamination resistant nucleic acid detecting cartridge according to claim 1, wherein the bottom plate is further provided with a sample buffer chamber and a test strip sample cell which are communicated with each other, and a sample pad end of the test strip is disposed in the test strip sample cell.

9. The nucleic acid contamination prevention nucleic acid detecting cartridge according to claim 8, wherein the base plate is further provided with a first air chamber and a second air chamber, the chip breaking structure is disposed in the first air chamber, and the absorbent pad end of the test strip is disposed in the second air chamber.

10. The nucleic acid contamination resistant nucleic acid detecting cartridge of claim 9, wherein the base plate comprises a first base plate and a second base plate, the chip breaking structure comprises a first protruding structure and a second protruding structure, the chip socket is located between the first base plate and the second base plate, the blister release module, the first protruding structure and the first air chamber are disposed on the first base plate, and the second protruding structure, the second air chamber, the sample buffer chamber, the test strip sample cell and the test strip are disposed on the second base plate.

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