CN114699343A

CN114699343A - Phellinus linteus fermentation product and preparation method thereof

Info

Publication number: CN114699343A
Application number: CN202210049751.7A
Authority: CN
Inventors: 阮华君; 陶俊俊; 唐长明; 詹国灿; 顾佳峰
Original assignee: Zhejiang Changshengniao Health Technology Co ltd
Current assignee: Zhejiang Changshengniao Health Technology Co ltd
Priority date: 2022-01-17
Filing date: 2022-01-17
Publication date: 2022-07-05
Anticipated expiration: 2042-01-17
Also published as: CN114699343B

Abstract

The present disclosure provides a preparation method of fomes fomentarius fermentation product, comprising: step one, activating, purifying and carrying out enlarged culture on fomes fomentarius to obtain seed liquid; inoculating the seed liquid to a fermentation substrate for fermentation culture treatment to obtain fermentation liquid; the fermentation substrate is potato homogenate; and step three, performing separation treatment and sterilization treatment on the fermentation liquor to obtain supernatant. Also disclosed are the resulting fomes fomentarius fermentates, including fomes fomentarius fermentation broth and dry powder thereof. In the preparation method of the fomes fomentarius fermentation product, fomes fomentarius is inoculated into the potato homogenate for fermentation, and the obtained fermentation product has good skin anti-inflammatory protection and repair effects and can be added into a cosmetic formula as an effective component.

Description

Phellinus linteus fermentation product and preparation method thereof

Technical Field

The disclosure belongs to the technical field of biological fermentation, and particularly relates to fomes fomentarius fermentation products and a preparation method thereof.

Background

Fomes fomentarius (Latin name is Fomes fomentarius), also called Fomes velutina, belonging to the class of Basidiomycetes, order of Polyporales, family of Polyporaceae, genus Fomes, is a white rot fungus, usually parasitized on broad-leaved trees such as birch, poplar, pear, apple, etc., and its fruit body is medicinal part, and can be used for removing food retention and removing blood stasis. Fomes fomentarius is a fungus with important application value, the sporocarp and the hypha of the fomes fomentarius contain various substances with biological activity, the representative substance is fomes fomentarius polysaccharide, and the polysaccharide is proved by Chinese and foreign medical research results to have the effects of resisting oxidation, promoting urination, reducing fever, relieving pain, diminishing inflammation, resisting tumors and the like.

However, reports on the application of fomes fomentarius to cosmetics are still blank at present. The inventor finds that the fermentation product obtained by the synergistic fermentation of fomes fomentarius and pearl has excellent skin anti-inflammatory repair effect and whitening effect, and can be used as an effective component for cosmetics.

Disclosure of Invention

The following presents a simplified summary of the disclosure in order to provide a basic understanding of some aspects of the disclosure. It should be understood that this summary is not an exhaustive overview of the disclosure. It is not intended to identify key or critical elements of the disclosure or to delineate the scope of the disclosure. Its sole purpose is to present some concepts in a simplified form as a prelude to the more detailed description that is discussed later.

In order to solve the technical problem, the technical scheme provided by the disclosure is as follows:

in a first aspect, the present disclosure provides a method for preparing a fomes fomentarius ferment, comprising:

step one, activating, purifying and carrying out enlarged culture on fomes fomentarius to obtain seed liquid;

inoculating the seed liquid to a fermentation substrate for fermentation culture treatment to obtain fermentation liquid; the fermentation substrate is potato homogenate;

and step three, performing separation treatment and sterilization treatment on the fermentation liquor to obtain supernatant.

Further, in the preparation method of the fomes fomentarius fermented product, pearl powder is also added into the fermentation substrate; the pearl powder content is 1-10 wt% (such as 2 wt%, 3 wt%, 4 wt%, 5 wt%, 6 wt%, 7 wt%, 8 wt%, 9 wt% etc.) of the potato homogenate; more preferably 1 to 5 wt% (e.g., 1.5 wt%, 2 wt%, 3 wt%, 4 wt%, 4.5 wt%, etc.).

Further, in the above method for producing a fomes fomentarius fermented product, the potato homogenate is obtained by homogenizing fresh cut potatoes with water by a homogenizer and dissolving, wherein the content of the potatoes is 1 to 5 wt% (e.g., 1.2 wt%, 1.5 wt%, 2.0 wt%, 2.5 wt%, 3 wt%, 3.5 wt%, 4 wt%, 4.5 wt%, etc.).

Further, in the above method for preparing fomes fomentarius fermented product, the potato homogenate further comprises 0.3 wt% of monopotassium phosphate, 0.15 wt% of magnesium sulfate, 0.05 wt% of glucose, and 0.2 wt% of soybean peptide.

The fermentation substrate is subjected to conventional sterilization treatment, such as high temperature sterilization at 115 ℃ for 20min, before inoculation.

Further, in the above method for producing a fomes fomentarius fermented product, the seed liquid has a hypha volume percentage of 80% or more (for example, 82%, 85%, 90%, etc.); the inoculation proportion of the Phellinus baumii, namely the volume ratio of the bacteria liquid to the pearl liquid fermentation medium (fermentation substrate) is 1-10% (such as 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, etc.).

Further, the fomes fomentarius is purchased from China general microbiological culture Collection center and is selected from at least one of the following: CGMCC5.1494, CGMCC 5.983, GCMCC5.96 or GCMCC 5.913.

Further, in the above method for preparing fomes fomentarius fermentation product, the temperature of the fermentation culture treatment is 25-35 deg.C (such as 26 deg.C, 28 deg.C, 30 deg.C, 32 deg.C, 34 deg.C, etc.), and the time is 48-96h (such as 52h, 60h, 72h, 84h, 88h, 92h, etc.).

Further, the fermentation culture process is carried out in a shaker at a speed of 150r/min to 200r/min (e.g., 155r/min, 160r/min, 165r/min, 170r/min, 180r/min, 190r/min, 195r/min, etc.).

Further, in the above method for producing a fomes fomentarius fermented product, the separation treatment is performed by a centrifugation method; more preferably, the centrifugation speed is 4500r/min-5500r/min (such as 4600r/min, 4800r/min, 5000r/min, 5200r/min, 5400r/min, etc.), and the centrifugation time is 10min-20min (such as 12min, 15min, 18min, etc.).

In a second aspect, the present disclosure provides a fomes fomentarius ferment obtained by the above preparation method. The fermentation product of fomes fomentarius Margarita comprises fomes fomentarius fermentation liquid and dry powder thereof, and the dry powder can be prepared by freeze drying or spray drying.

In a third aspect, the present disclosure also provides a cosmetic containing the fomes fomentarius fermentation product. The cosmetic may be facial mask, essence, cream, lotion, etc.

Compared with the prior art, the beneficial effects of the present disclosure include but are not limited to:

1. the fomes fomentarius fermentation product is prepared by inoculating fomes fomentarius into potato homogenate for fermentation, and the obtained fermentation product has good skin anti-inflammatory protection and repair effects;

2. according to the preparation method of the fomes fomentarius fermentation product, the fomes fomentarius and the pearls are subjected to synergistic fermentation to prepare the fermentation product with excellent anti-inflammatory performance and whitening performance, and the fermentation product can be used as an effective component to be added into a cosmetic formula to prepare cosmetics such as a facial mask, essence, sunscreen cream and emulsion;

3. the fomes fomentarius fermentation product provided by the disclosure as a cosmetic efficacy component has the characteristics of strong efficacy, low cost, simple operation, green safety and the like, and has strong practicability and popularization value.

Drawings

FIG. 1 is a schematic diagram of the anti-inflammatory effect detection result of the protection treatment of the fomes fomentarius pearl fermentation product in example 2, and indexes are as follows: IL-1 β;

fig. 2 is a schematic diagram of the anti-inflammatory effect test results of the protection treatment of the pearl potato extract in example 2, with the following indexes: IL-1 β;

FIG. 3 is a schematic diagram of the protection treatment anti-inflammatory effect detection results of different concentrations of fomes fomentarius pearl fermentation in example 2, and the indexes are as follows: IL-1 β;

FIG. 4 is a schematic diagram of the detection result of the anti-inflammatory effect of the restoration treatment of the fomes fomentarius pearl fermentation product in example 2, and the indexes are as follows: IL-1 β;

fig. 5 is a schematic diagram of the detection result of the repair treatment anti-inflammatory effect of the pearl potato extract in example 2, and indexes are as follows: IL-1 β;

FIG. 6 is a schematic diagram of the detection results of the repair treatment anti-inflammatory effect of the fomes fomentarius pearl fermentation products with different concentrations in example 2, and the indexes are as follows: IL-1 β;

fig. 7 is a schematic diagram of the detection result of the repair treatment anti-inflammatory effect of the pearl and potato extract in example 2, and the indexes are as follows: IL-8;

FIG. 8 is a schematic diagram of the detection results of the repair treatment anti-inflammatory effect of the fomes fomentarius pearl fermentation products with different concentrations in example 2, and the indexes are as follows: IL-8.

Detailed Description

The following examples further illustrate the present invention but are not to be construed as limiting the invention. Modifications or substitutions to methods, steps or conditions of the present invention may be made without departing from the spirit and scope of the invention.

The technical solutions of the present disclosure will be described below with reference to exemplary embodiments. The experimental procedures used in the following examples are all conventional procedures unless otherwise specified. Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.

The fomes fomentarius used in the examples is a commercial product purchased from China general microbiological culture Collection center with the preservation numbers: CGMCC 5.1494.

Example 1 preparation of Phellinus linteus fermentation product

(1) Activating strains: selecting a colony from a preserved fomes fomentarius CGMCC5.1494 (laboratory number 5372) inclined plane, culturing the colony in a glucose potato agar culture medium at 28 ℃ for 7d for activation, then inoculating the obtained single colony to 100mL of glucose potato liquid culture medium, culturing at 28 ℃ and 180rpm for 7d to obtain fomes fomentarius seed liquid, wherein the volume ratio of mycelium accounts for 80% of the whole seed liquid.

(2) Preparing a fermentation substrate: according to the settings of the following table 1, a certain mass ratio of pearl powder (1-10 wt%, such as 1 wt%, 5 wt%, 10 wt%) is added to potato homogenate, wherein the potato homogenate is prepared by adding 1000ml of mineral water (containing 0.3% of potassium dihydrogen phosphate, 0.15% of magnesium sulfate, 0.05% of glucose, 0.2% of soybean peptide) into 20g of cut potatoes, homogenizing, dissolving, and packaging. The fermentation substrate is sterilized prior to inoculation.

(3) Inoculating and shaking bacteria: according to the inoculation amount of 5 percent by volume, 5mL of the seed solution is inoculated into a 250mL triangular flask containing 100mL of fermentation substrate, and the mixture is fermented in a shaking table at the rotating speed of 180r/min, the culture temperature of 28 ℃ and the fermentation time of 48 h.

(4) Centrifuging: centrifuging the fermentation broth at 4800r/min for 10min, collecting supernatant, and sterilizing to obtain sample numbers shown in Table 1.

In this example, a control group was also provided, and the simple fermentation substrate was the above-mentioned 2% potato homogenate to which 0.3% monopotassium phosphate, 0.15% magnesium sulfate, 0.05% glucose, and 0.2% soybean peptide were added. See table 1 for details.

TABLE 1 fermentation System and Experimental group designations for example 1

Number of experimental group	Fermentation substrate	Inoculation situation
			TD-0	Substrate for simple fermentation	Without inoculation
TD-5372	Substrate for simple fermentation	Inoculation of 5372 Strain
			1％ZZ-TD-5372	Fermentation substrate containing 1% of pearl powder	Inoculation of 5372 Strain
5％ZZ-TD-5372	Fermentation substrate containing 5% of pearl powder	Inoculation of 5372 Strain
			10％ZZ-TD-5372	Fermentation substrate containing 10% of pearl powder	Inoculation of 5372 Strain
1％ZZ-TD	Fermentation substrate containing 1% of pearl powder	Without inoculation
			5％ZZ-TD	Fermentation substrate containing 5% of pearl powder	Without inoculation
10％ZZ-TD	Fermentation substrate containing 10% of pearl powder	Without inoculation

The main components of the product prepared in example 1, including protein, amino acid and calcium content, were measured, and the results are shown in tables 2 to 4 below.

TABLE 2 amino acid content (umol/mL) of the stock solution or fermentation stock solution samples

TABLE 3 protein content (ug/mL) of the stock or fermentation stock samples

TABLE 4 calcium content of stock solution or fermentation stock solution

Number of experimental group	Calcium (mg/kg)	Number of experimental group	Calcium (mg/kg)
				TD-0	12.4	TD-5372	1.3
1％ZZ-TD	39.0	1％ZZ-TD-5372	179.5
				5％ZZ-TD	33.1	5％ZZ-TD-5372	167.7
10％ZZ-TD	28.2	10％ZZ-TD-5372	25.3

Example 2 efficacy analysis of fomes fomentarius fermentate

Toxicity and protective action of fomes fomentarius fermentation product on human skin fibroblast (HSF cell)

1. Establishment of oxidative stress model

HSF cells were plated at 1X 10 per well⁴Inoculating into 96-well plate, culturing overnight in cell culture box, discarding culture medium, and adding 100 μ L of different concentrations of H₂O₂(50～1000μmol·L^-1) The culture medium of (1) was repeated 5 times for each group, and no H was added to the control group₂O₂. After 1, 2, 3, 4h of stimulation, the OD of each well was measured at 490nm using the MTT method. The survival rate of HSF cells (see figure 1) is obtained, and the hydrogen peroxide concentration with the cell survival rate of 50% is selected as the modeling concentration. In this example, 100. mu. mol. L^-1H of (A)₂O₂The HSF cells are treated for 2h for modeling, and the cell survival rate is (49.74 +/-2.99)%.

2. Sample pair H₂O₂Protection and repair testing of induced oxidative stress model

Refining HSFCells at 1X 10 per well⁴Each of the cells was inoculated in a 96-well plate and cultured overnight in a cell incubator, and a blank Control group (Control), a Model group (Model), and each sample group (stock solution or fermentation stock solution in example 1) were set for each experiment, and 3 replicates were set for each group.

The detection of the repairing effect means that after the cells are damaged, the samples are used for acting, and whether the samples have the repairing effect is evaluated by measuring the content of IL-1 beta and the like. The repair treatment group in this embodiment: adding H first₂O₂After 2h of stimulation, 100 mu L of sample is added into each hole for culturing for 24 h;

and (3) protective effect detection, namely, after the sample acts on the cells, damaging the cells again, observing isosecretion conditions of IL-1 beta of the cells, and judging whether the sample has a protective effect. Protection processing group in this embodiment: adding the sample to culture for 24H, and adding H₂O₂And stimulating for 2 hours.

Testing indexes are as follows: IL-1. beta. and IL-8 (test method refers to kit instructions). The results are shown in FIGS. 1-8.

FIG. 1 is a schematic diagram of the anti-inflammatory effect test results of the protection treatment of fomes fomentarius fermentation product in example 2, and the indexes are as follows: IL-1 beta. As can be seen from the figure, IL-1 beta of the Model group (Model) is extremely higher than that of the blank Control group (Control), which indicates that the Model is successfully established; compared with the model group, the 1% ZZ-TD has no significant difference, and after inoculation and fermentation, the 1% ZZ-TD-5372 is extremely lower than the model group, which shows that the fermentation liquor after inoculation is helpful to reduce the secretion of IL-1 beta and has anti-inflammatory effect.

Fig. 2 is a schematic diagram of the anti-inflammatory effect test results of the protection treatment of the pearl potato extract in example 2, with the following indexes: IL-1 beta. From the figure, the IL-1 beta of the model group is remarkably higher than that of the blank control group, which shows that the model is successfully established, and the influence change difference of the pearl and potato extracts with different proportions on the IL-1 beta is large. When the pearl proportion is 0, the effect of IL-1 beta is not reduced, which shows no anti-inflammatory effect, the pearl potato extract added with 1% -5% of pearl powder has extremely obvious effect of reducing IL-1 beta, which shows anti-inflammatory effect, and when the pearl proportion is increased to 10%, the influence on IL-1 beta is not obviously different, and the pearl potato extract does not have anti-inflammatory effect.

FIG. 3 is a schematic diagram of the protection treatment anti-inflammatory effect detection results of different concentrations of fomes fomentarius pearl fermentation in example 2, and the indexes are as follows: IL-1 beta. The graph shows that the IL-1 beta of the model group is remarkably higher than that of the blank control group, which indicates that the model is successfully established, 5372 the fermentation product inoculated into the potato culture medium without pearl addition can also remarkably reduce the secretion of the IL-1 beta and has anti-inflammatory effect; when 1-5% of pearls are added according to the proportion, the anti-inflammatory effect is more obvious; but inoculation of 5372 when added to 10% pearls is not able to reduce IL-1 β.

FIG. 4 is a schematic diagram of the detection result of the anti-inflammatory effect of the restoration treatment of the fomes fomentarius pearl fermentation product in example 2, and the indexes are as follows: IL-1 beta. It can be seen from the figure that the IL-1 beta of the model group is significantly higher than that of the blank control group, which indicates that the model is successfully established, and compared with the effects on the IL-1 beta of the potato extract with the same pearl addition ratio before and after fermentation, the fermentation product after inoculation of 5372 has significant effect of reducing the IL-1 beta, but does not have the effect of reducing the IL-1 beta before fermentation.

Fig. 5 is a schematic diagram of the detection result of the repair treatment anti-inflammatory effect of the pearl and potato extract in example 2, and the indexes are as follows: IL-1 beta. From the figure, the IL-1 beta of the model group is extremely higher than that of the blank control group, which indicates that the model is successfully established; the pearl potato extract with 5% of pearl addition rate has repairing effect, while the pearl potato extract with 1% of pearl addition rate and 10% of pearl addition rate has no reduction effect on IL-1 beta.

FIG. 6 is a schematic diagram of the detection results of the repair treatment anti-inflammatory effect of the fomes fomentarius pearl fermentation products with different concentrations in example 2, and the indexes are as follows: IL-1 beta. From the figure, the IL-1 beta of the model group is extremely higher than that of the blank control group, which represents that the model is successfully established; the secretion of IL-1 beta cannot be reduced in the potato culture medium without pearl addition before or after fermentation, and the potato culture medium has no anti-inflammatory repair effect; when 1-5% of pearls are added according to the proportion, the anti-inflammatory repair effect is obvious; however, inoculation of 5372 fermentation product when added to 10% pearls is not able to reduce IL-1 β.

Fig. 7 is a schematic diagram of the detection result of the repair treatment anti-inflammatory effect of the pearl and potato extract in example 2, and the indexes are as follows: IL-8. From the figure, it can be seen that the IL-8 in the model group is extremely higher than that in the blank control group, which indicates that the model is successfully established; the pearl-potato extract with 10% of pearl addition has IL-8 reducing effect, i.e. repairing effect, but the potato extract with 1% and 5% of pearl addition has no anti-inflammatory effect.

FIG. 8 is a schematic diagram of the detection results of the repair treatment anti-inflammatory effect of the fomes fomentarius pearl fermentation products with different concentrations in example 2, and the indexes are as follows: IL-8. As can be seen from the figure, the IL-8 in the model group is extremely higher than that in the blank control group, which indicates that the model is successfully established; the 5372 potato fermentation liquor without pearl has IL-8 reducing effect, which indicates that the potato fermentation liquor has repairing effect; the potato fermentation liquor with the pearl addition amount of 1-5% also has the effect of reducing IL-8, which shows that the potato fermentation liquor has a repairing effect; when the addition was increased to 10%, there was no repairing effect (no significant effect on IL-8).

Second, the tyrosinase inhibitory activity test of fomes fomentarius fermentation product

1. Principle of experiment

The formation of melanin is that tyrosine in skin melanocyte tissue is gradually converted into true melanin through intermediates such as dopa, dopaquinone, dopachrome and dihydroxyindole under the action of enzymes such as tyrosinase and the like; furthermore, melanin is transferred into keratin cells by melanocyte tissues and is shed along with the turnover of the stratum corneum, and the difference of human skin color mainly depends on the content and distribution of the true melanin and the pheomelanin, but when the true melanin is excessively increased and unevenly distributed, local skin hyperdarkening or pigmentation is caused. There are three major enzymes involved in melanin synthesis: tyrosinase, dopachrome tautomerase and dihydroxyindole carboxylate oxidase. Tyrosinase belongs to oxidoreductase and is the main rate-limiting enzyme for melanin synthesis, the activity of the oxidoreductase determines the formation amount of melanin, and many whitening and freckle-removing products sold on the market achieve the whitening effect by inhibiting the activity of tyrosinase, so the strength of the inhibition effect on the tyrosinase is the main index for evaluating whitening cosmetics. The L-tyrosinase and its substrate L-tyrosine can be reacted catalytically. After a reagent with the L-tyrosinase activity inhibition effect is added into an experimental system, the inhibition effect can be generated on a catalytic reaction, and the inhibition rate of the reagent on the L-tyrosinase activity is evaluated by measuring the absorbance at 475nm before and after the reagent is added.

2. Preparation of the experiment

1) PBS phosphate buffer (pH 6.8): dissolving 17.91g of dodecahydrate disodium hydrogen phosphate in distilled water, and dissolving in 500 mL; dissolving 7.8g of dihydrate sodium dihydrogen phosphate in distilled water, and dissolving in 500 mL; the two solutions, 92.6mL and 107.4mL, were combined to 200mL of PBS phosphate buffer, pH 6.8.

2)0.1mol/L hydrochloric acid solution: 0.862mL of HCl solution with the HCl content of 36% -38% is used, and the volume is fixed to 100mL by distilled water.

3) 0.05% L-tyrosine solution: 0.05g L-tyrosine was dissolved in 35mL of 0.1mol/L HCl solution, and then 65mL of PBS phosphate buffer (pH6.8) was added thereto to make 100 mL.

4) Tyrosinase solution: diluting the tyrosinase powder with PBS buffer solution to obtain tyrosinase solution with enzyme activity of 100U/mL.

3. Experimental procedure

1) After the C2 pipe is well prepared and shaken up, the mixture is heated in a water bath kettle at 37 ℃ for 10min, and the zero setting is carried out under the wavelength of 475 nm.

2) Mixing the solution in a C1 tube, shaking, performing water bath at 37 ℃ for 10min, adding 1ml of tyrosinase, continuing the water bath for 10min, and determining the absorbance value of C1.

3) The absorbance values of T1 were determined by zeroing with T2 in the same manner as in 1) and 2).

4) And calculating the activity inhibition rate T (%) of the fermentation stock solution sample on the tyrosinase.

TABLE 5 tyrosinase inhibition ratio test reagent proportioning table

Numbering	L-tyrosine	Sample (I)	PBS	Tyrosinase enzyme	Total volume
						C1	2mL	——	4mL	1mL	7ml
C2	2mL	——	5ml	——	7ml
						T1	2ml	2mL	2mL	1mL	7ml
T2	2ml	2ml	3ml	——	7ml

4. Data processing

The formula: t (%) - (C1-T1)/C1 × 100%. The results are shown in Table 6, and the data in the table show that the fomes fomentarius and the pearl are fermented, and the two have synergistic effect, so that the whitening performance of the fermented product is remarkably improved.

TABLE 6 tyrosinase inhibition test results

Finally, it is further noted that, in the present disclosure, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

While the disclosure has been disclosed above by the description of specific embodiments thereof, it should be understood that various modifications, improvements or equivalents of the disclosure may be devised by those skilled in the art within the spirit and scope of the appended claims. Such modifications, improvements and equivalents are intended to be included within the scope of the present disclosure as claimed.

Claims

1. A preparation method of fomes fomentarius fermentation product is characterized by comprising the following steps:

2. The method of preparing fomes fomentarius ferment as claimed in claim 1, wherein the potato homogenate is obtained by homogenizing and dissolving fresh cut potatoes in water by a homogenizer, wherein the content of the potatoes is 1 to 5 wt%.

3. The method for producing a fomes fomentarius fermented product according to claim 1 or 2, wherein the potato homogenate further comprises 0.3 wt% of monopotassium phosphate, 0.15 wt% of magnesium sulfate, 0.05 wt% of glucose, and 0.2 wt% of soybean peptide.

4. The method for preparing fomes fomentarius fermented product according to any one of claims 1 to 3, wherein pearl powder is further added to the fermentation substrate; the content of the pearl powder is 1-10 wt% of the potato homogenate; preferably 1 to 5 wt%.

5. The method for producing a fomes fomentarius fermented product according to any one of claims 1 to 4, wherein in the first step, the hypha volume percentage of the seed liquid is 80% or more.

6. The method for preparing fomes fomentarius ferment according to any one of claims 1 to 5, wherein in the second step, the volume ratio of the seed solution to the fermentation substrate is 1% to 10%.

7. The method for preparing fomes fomentarius ferment according to any one of claims 1 to 6, wherein in the second step, the temperature of the fermentation culture treatment is 25 to 35 ℃ and the time is 48 to 96 hours; preferably, the fermentation culture treatment is carried out in a shaker at a rotation speed of 150r/min to 200 r/min.

8. The method for producing a fomes fomentarius ferment according to any one of claims 1 to 7, wherein the separation treatment employs a centrifugation method; preferably, the centrifugal speed is 4500r/min-5500r/min, and the centrifugal time is 10min-20 min.

9. The method for producing fomes fomentarius ferment according to any one of claims 1 to 8, wherein the fomes fomentarius is selected from at least one of: CGMCC5.1494, CGMCC 5.983, GCMCC5.96 and GCMCC 5.913.

10. A fomes fomentarius fermented product, which is produced by the production method according to any one of claims 1 to 9; the fomes fomentarius fermentation product is fomes fomentarius fermentation liquor and dry powder thereof.