CN114680328A - Preparation method of rhizoma polygonati chewable pills added with dendrobium officinale - Google Patents
Preparation method of rhizoma polygonati chewable pills added with dendrobium officinale Download PDFInfo
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- NRYBAZVQPHGZNS-ZSOCWYAHSA-N leptin Chemical compound O=C([C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)CC(C)C)CCSC)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CS)C(O)=O NRYBAZVQPHGZNS-ZSOCWYAHSA-N 0.000 abstract description 6
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/385—Concentrates of non-alcoholic beverages
- A23L2/39—Dry compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
Description
技术领域technical field
本发明涉及保健食品加工技术领域,尤其是一种添加铁皮石斛的黄精咀嚼丸的制作方法。The invention relates to the technical field of health food processing, in particular to a preparation method of Polygonum chinensis chewing pills added with Dendrobium officinale.
背景技术Background technique
黄精,中药名。为百合科植物滇黄精Polygonatum kingianum Coll.et Hemsl.、黄精Polygonatum sibiricum Red.或多花黄精Polygonatum cyrtonema Hua的干燥根茎。按形状不同,习称“大黄精”、“鸡头黄精”、“姜形黄精”。具有补气养阴,健脾,润肺,益肾之功效。常用于脾胃气虚,体倦乏力,胃阴不足,口干食少,肺虚燥咳,劳嗽咳血,精血不足,腰膝酸软,须发早白,内热消渴。Huangjing, Chinese medicine name. It is the dried rhizomes of Liliaceae Polygonatum kingianum Coll.et Hemsl., Polygonatum sibiricum Red. or Polygonatum cyrtonema Hua. According to different shapes, they are known as "Rhubarb Extract", "Chicken Head Rhizoma Extract" and "Ginger-shaped Rhizoma Extract". It has the effects of tonifying qi and nourishing yin, strengthening the spleen, moistening the lungs, and benefiting the kidneys. Commonly used for deficiency of spleen and stomach qi, fatigue and fatigue, deficiency of stomach yin, dry mouth and lack of food, dry cough due to lung deficiency, coughing up blood due to labor and cough, lack of essence and blood, soreness and weakness of waist and knees, premature graying of beard and hair, internal heat and quenching thirst.
铁皮石斛(学名:Dendrobium officinale Kimura et Migo):茎直立,圆柱形,长9-35厘米,粗2-4毫米,不分枝,具多节;叶二列,纸质,长圆状披针形,边缘和中肋常带淡紫色。总状花序常从落了叶的老茎上部发出,具2-3朵花;花苞片干膜质,浅白色,卵形,长5-7毫米,萼片和花瓣黄绿色,近相似,长圆状披针形,唇瓣白色,基部具1个绿色或黄色的胼胝体,卵状披针形,比萼片稍短,中部反折。蕊柱黄绿色,长约3毫米,先端两侧各具1个紫点;药帽白色,长卵状三角形,长约2.3毫米,顶端近锐尖并且2裂。花期3-6月。Dendrobium officinale (scientific name: Dendrobium officinale Kimura et Migo): stems erect, cylindrical, 9-35 cm long, 2-4 mm thick, unbranched, with many nodes; leaves two rows, papery, oblong-lanceolate , margins and midrib often lavender. The racemes often emerge from the upper part of the old stem with deciduous leaves, with 2-3 flowers; flower bracts dry membranous, pale white, ovate, 5-7 mm long, yellow-green sepals and petals, nearly similar, oblong lanceolate, white labial, base with a green or yellow corpus callosum, ovate-lanceolate, slightly shorter than sepals, centrally reflexed. The stamen is yellow-green, about 3 mm long, with a purple spot on both sides of the apex; the cap is white, long ovate-triangular, about 2.3 mm long, the top is nearly acute and 2-lobed. Flowering March-June.
黄精和石斛都是现有中药栽培中规模较大的中药,如何深度开发提高市场普及程度是当前急需解决的问题。相当多的研究侧重于将黄精和石斛制作成饮料,但是为了保证饮料风味和口感,导致提取中原料有相当程度的浪费,也不能针对黄精和石斛本身的纤维特点进行利用,导致资源化程度低。尤其是当前减肥市场巨大,将黄精和石斛结合促进生物体脂肪代谢是极有前景的方向,所以如何深度开发并且提高原料使用效率制作出适宜减肥人群的产品成为当前技术中急需解决的问题。Huang Jing and Dendrobium are both large-scale traditional Chinese medicines in the existing traditional Chinese medicine cultivation. How to deeply develop and improve the market popularity is an urgent problem to be solved at present. Quite a lot of research has focused on the production of Polygonum and Dendrobium into beverages, but in order to ensure the flavor and taste of the beverages, the raw materials in the extraction are wasted to a considerable extent, and the fiber characteristics of Polygonatum and Dendrobium themselves cannot be utilized, resulting in a low degree of resource utilization. . In particular, the current weight loss market is huge. It is a very promising direction to combine Huang Jing and Dendrobium to promote the metabolism of body fat. Therefore, how to deeply develop and improve the use efficiency of raw materials to make products suitable for people who lose weight has become an urgent problem to be solved in the current technology.
发明内容SUMMARY OF THE INVENTION
为了解决现有技术中存在的问题,本发明提供一种添加铁皮石斛的黄精咀嚼丸的制作方法,以促进黄精和石斛的开发,增加其产品形态,具体技术方案如下:In order to solve the problems existing in the prior art, the present invention provides a kind of preparation method of the Polygonum chinensis chewing pills added with Dendrobium officinale, to promote the development of Polygonatum chinensis and Dendrobium officinale, and increase its product form, and the concrete technical scheme is as follows:
一种添加铁皮石斛的黄精咀嚼丸的制作方法,包括如下步骤:A preparation method of Huangjing chewable pills with Dendrobium officinale, comprising the steps:
(1)原料提取(1) Raw material extraction
A制备酸性酶化提取液A Preparation of acid enzymatic extract
以质量份计,将20-30份杨梅、8-13份广枣、12-17份山楂、4-7份鱼粉、10-15份麦芽混合粉碎磨浆,在120-140℃下灭菌2-4h,将混合物恢复至常温,接种酿酒酵母菌,在28-31℃下密封发酵5-8h,接种醋酸杆菌,在30-33℃下发酵70-80h,加水稀释2-3倍,继续发酵10-23h,稀释3-5倍后抽滤即得所述酶化提取液;In parts by mass, 20-30 parts of bayberry, 8-13 parts of jujube, 12-17 parts of hawthorn, 4-7 parts of fish meal, 10-15 parts of malt are mixed, crushed and refined, and sterilized at 120-140 ° C for 2 -4h, return the mixture to room temperature, inoculate Saccharomyces cerevisiae, seal and ferment at 28-31°C for 5-8h, inoculate Acetobacter, ferment at 30-33°C for 70-80h, add water to dilute 2-3 times, and continue fermentation 10-23h, after diluting 3-5 times, suction filtration to obtain the enzymatic extract;
B铁皮石斛提取B Dendrobium officinale Extract
将新鲜铁皮石斛的粉碎,加入上述A制得的酶化提取液混合在35-38℃下浸泡20-30min,自然冷却至室温,用500-700W的微波加热至45-48℃,保温13-18min,搅拌30-50min,然后抽滤收集滤液;将滤渣与其质量5-10倍的食用乙醇混合,用700-900W的微波加热至55-60℃,浸泡10-18min,自然降温至室温,用700-900W的微波加热至65-68℃,保温8-10min,将温度降温至45-50℃,浸泡1-3h,过滤,合并两次滤液浓缩烘干得到铁皮石斛提取物;Pulverize fresh Dendrobium officinale, add the enzymatic extract obtained from A above, mix and soak at 35-38°C for 20-30min, naturally cool to room temperature, heat to 45-48°C with 500-700W microwave, and keep the temperature for 13- 18min, stir for 30-50min, then collect the filtrate by suction filtration; mix the filter residue with 5-10 times the mass of edible ethanol, heat it to 55-60°C with a microwave of 700-900W, soak for 10-18min, cool down to room temperature naturally, and use 700-900W microwave heating to 65-68 ℃, keeping the temperature for 8-10 minutes, cooling the temperature to 45-50 ℃, soaking for 1-3 hours, filtering, combining two filtrates, concentrating and drying to obtain Dendrobium officinale extract;
C黄精提取C Polygonatum Extract
将黄精捣碎,加入A中酶化提取液混合搅拌10-15min,在零下20-25℃下冷冻5-9h,取出用碎冰机粉碎,将粉碎物加热至5-10℃,用600-700W的超声波处理20-25min,在40-43℃下静置50-80min过滤,收集滤液,将滤液浓缩烘干即得黄精提取物;Mash the Polygonatum glutinosa, add the enzymatic extract in A, mix and stir for 10-15min, freeze at -20-25℃ for 5-9h, take out and pulverize with an ice crusher, heat the pulverized material to 5-10℃, use 600- Ultrasonic treatment of 700W for 20-25min, stand at 40-43°C for 50-80min to filter, collect the filtrate, concentrate and dry the filtrate to obtain Polygonatum chinensis extract;
(2)原料调制(2) Raw material preparation
将上一步骤中的提取黄精剩的滤渣和提取铁皮石斛剩的滤渣按照质量比23:8-11混合,加入糯玉米粉,调节含水量为75-88%,接种乳杆菌,发酵18-22h,搅拌均匀即可;Mix the remaining filter residue from the extraction of Polygonatum chinensis in the previous step and the residue from the extraction of Dendrobium officinale according to a mass ratio of 23:8-11, add waxy corn flour, adjust the water content to 75-88%, inoculate with Lactobacillus, and ferment for 18-22h , stir evenly;
(3)原料蒸晒(3) Steaming and drying of raw materials
将步骤(2)中物料在90-95℃下蒸80-100min,制作成直径1-1.5cm的圆球,放置到太阳下暴晒2-3天,然后收集放回蒸笼中蒸2-3h,暴晒1-2天,再次蒸2-3h晾凉即可;The material in step (2) is steamed at 90-95°C for 80-100min, made into a ball with a diameter of 1-1.5cm, placed in the sun for 2-3 days, and then collected and put back in a steamer to steam for 2-3h, Expose to the sun for 1-2 days, steam again for 2-3 hours and let it cool;
(4)原料成型(4) Raw material molding
将步骤(1)中铁皮石斛提取物和黄精提取物按照质量1:3混合,稀释3-5倍,加入海藻酸钠、果胶混合均匀得到强化液,将步骤(3)制作的圆球浸没到强化液中1-2min,捞出沥干水分,再次浸没到强化液中2-3min,捞出在40-45℃下减压干燥即可得到所述咀嚼丸;In the step (1), the Dendrobium officinale extract and the Polygonatum chinensis extract are mixed according to mass 1:3, diluted 3-5 times, sodium alginate and pectin are added and mixed evenly to obtain a strengthening solution, and the sphere made in step (3) is immersed Put it into the strengthening solution for 1-2 minutes, take out and drain the water, immerse it again in the strengthening solution for 2-3 minutes, take it out and dry it under reduced pressure at 40-45°C to obtain the chewable pills;
所述海藻酸钠的用量为黄精提取物质量的25-30%;所述果胶的用量为黄精提取物质量的13-18%;。The dosage of the sodium alginate is 25-30% of the mass of the Polygonatum chinensis extract; the dosage of the pectin is 13-18% of the mass of the Polygonatum chinensis extract;
进一步的,步骤(1)中,所述麦芽的制作方法为:将大麦和小麦按照质量比1:0.2-0.33混合,用质量分数0.0003-0.0008%的硒酸钠溶液混合,浸泡5-8h,调节含水量为88-90%,在15-18℃下催芽至胚芽鞘突破种皮3-5mm即可。Further, in step (1), the preparation method of the malt is as follows: mixing barley and wheat according to a mass ratio of 1:0.2-0.33, mixing with a sodium selenate solution with a mass fraction of 0.0003-0.0008%, soaking for 5-8h, Adjust the water content to 88-90%, and germinate at 15-18°C until the coleoptile breaks through the seed coat by 3-5mm.
进一步的,步骤(1)中,所述酿酒酵母的接种量为混合物质量的5-9%。Further, in step (1), the inoculation amount of the Saccharomyces cerevisiae is 5-9% of the mass of the mixture.
进一步的,步骤(1)中,所述醋酸杆菌的接种量为混合物质量的3-8%。Further, in step (1), the inoculum of the Acetobacter is 3-8% of the mass of the mixture.
进一步的,步骤(1)中,所述铁皮石斛和酶化提取液的质量比为1:5-8。Further, in step (1), the mass ratio of the Dendrobium officinale and the enzymatic extract is 1:5-8.
进一步的,步骤(1)中,所述搅拌的速率为80-100r/min。Further, in step (1), the stirring speed is 80-100r/min.
进一步的,步骤(1)中,所述黄精和酶化提取液的质量比为1:10-13。Further, in step (1), the mass ratio of the Polygonatum chinensis to the enzymatic extract is 1:10-13.
进一步的,步骤(2)中,所述糯玉米粉和混合滤渣的质量比为1:5-7。Further, in step (2), the mass ratio of the waxy corn flour and the mixed filter residue is 1:5-7.
进一步的,步骤(1)中,所述乳杆菌的用量为发酵底物质量的2-4%。Further, in step (1), the dosage of the Lactobacillus is 2-4% of the mass of the fermentation substrate.
进一步的,步骤(1)中,所述发酵温度为25-28℃。Further, in step (1), the fermentation temperature is 25-28°C.
与现有技术相比,本发明的技术效果体现在:Compared with the prior art, the technical effect of the present invention is embodied in:
本发明通过针对性的制作提取液,利用酿酒酵母发酵产生酒精,促进醋酸菌的酸发酵,加之菌体代谢产生的活性酶,使得提取液中形成酸-醇-酶的混合提取体系,以此作为提取溶剂,提取黄精和石斛中的有效成分。用剩下的滤渣蒸晒形成高纤维的丸体,吸附提取组分形成咀嚼丸,避免直接将黄精、石斛高温制作导致的营养损失,而且多次蒸晒使得咀嚼丸的纤维固化,提高咀嚼口感,也能形成抗消化的纤维丛,有效促进肠胃蠕动促进脂肪的分解。而且提取出来的黄精、石斛组分也能促进瘦素形成,达到对脂肪的多重抑制,提高减肥效果。In the present invention, through the targeted preparation of the extract, the fermentation of Saccharomyces cerevisiae is used to produce alcohol, the acid fermentation of the acetic acid bacteria is promoted, and the active enzymes produced by the metabolism of the bacteria are added, so that a mixed extraction system of acid-alcohol-enzyme is formed in the extract. As an extraction solvent, extract the active ingredients in Polygonatum and Dendrobium. The remaining filter residues are steamed to form high-fiber pellets, and the extracted components are adsorbed to form chewing pills, avoiding the nutrient loss caused by the direct production of Polygonatum and Dendrobium at high temperature, and multiple times of steaming and drying to solidify the fibers of the chewing pills and improve the chewing taste. It can also form anti-digestive fiber bundles, effectively promote gastrointestinal motility and promote the decomposition of fat. Moreover, the extracted components of Polygonatum chinensis and Dendrobium can also promote the formation of leptin, achieve multiple inhibition of fat, and improve the effect of weight loss.
本发明的咀嚼丸可以直接咀嚼食用,也能用开水冲泡引用,方便容易携带,可以满足多种人群。The chewable pills of the present invention can be directly chewed and eaten, and can also be brewed with boiling water for reference, which is convenient and easy to carry, and can satisfy various groups of people.
具体实施方式Detailed ways
下面结合具体的实施方式来对本发明的技术方案做进一步的限定,但要求保护的范围不仅局限于所作的描述。The technical solutions of the present invention will be further limited below in conjunction with specific embodiments, but the scope of protection is not limited to the description.
实施例1Example 1
一种添加铁皮石斛的黄精咀嚼丸的制作方法,包括如下步骤:A preparation method of Huangjing chewable pills with Dendrobium officinale, comprising the steps:
(1)原料提取(1) Raw material extraction
A制备酸性酶化提取液A Preparation of acid enzymatic extract
以质量份计,将20份杨梅、8份广枣、12份山楂、4份鱼粉、10份麦芽混合粉碎磨浆,在120℃下灭菌2h,将混合物恢复至常温,接种酿酒酵母菌,在28℃下密封发酵5h,接种醋酸杆菌,在30℃下发酵70h,加水稀释2倍,继续发酵10h,稀释3倍后抽滤即得所述酶化提取液;In parts by mass, 20 parts of bayberry, 8 parts of jujube, 12 parts of hawthorn, 4 parts of fish meal, and 10 parts of malt were mixed, crushed and refined, sterilized at 120 ° C for 2 hours, the mixture was returned to normal temperature, and inoculated with Saccharomyces cerevisiae, Sealed and fermented at 28°C for 5h, inoculated with Acetobacter, fermented at 30°C for 70h, diluted 2 times with water, continued to ferment for 10h, diluted 3 times and filtered to obtain the enzymatic extract;
所述麦芽的制作方法为:将大麦和小麦按照质量比1:0.2混合,用质量分数0.0003%的硒酸钠溶液混合,浸泡5h,调节含水量为88%,在15℃下催芽至胚芽鞘突破种皮3mm即可;所述酿酒酵母的接种量为混合物质量的5%;所述醋酸杆菌的接种量为混合物质量的3%;The preparation method of the malt is as follows: mixing barley and wheat according to a mass ratio of 1:0.2, mixing with a sodium selenate solution with a mass fraction of 0.0003%, soaking for 5 hours, adjusting the water content to 88%, and accelerating germination to coleoptiles at 15° C. Break through the seed coat by 3 mm; the inoculum of the Saccharomyces cerevisiae is 5% of the mass of the mixture; the inoculum of the Acetobacter is 3% of the mass of the mixture;
B铁皮石斛提取B Dendrobium officinale Extract
将新鲜铁皮石斛的粉碎,加入上述A制得的酶化提取液混合在35℃下浸泡20min,自然冷却至室温,用500W的微波加热至45℃,保温13min,搅拌30min,然后抽滤收集滤液;将滤渣与其质量5倍的食用乙醇混合,用700W的微波加热至55℃,浸泡10min,自然降温至室温,用700W的微波加热至65℃,保温8min,将温度降温至45℃,浸泡1h,过滤,合并两次滤液浓缩烘干得到铁皮石斛提取物;Pulverize fresh Dendrobium officinale, add the enzymatic extract obtained from A above, mix and soak at 35°C for 20min, naturally cool to room temperature, heat to 45°C with 500W microwave, keep warm for 13min, stir for 30min, and then collect the filtrate by suction filtration ; Mix the filter residue with 5 times its mass of edible ethanol, heat it to 55°C with a 700W microwave, soak for 10 minutes, cool down to room temperature naturally, heat it to 65°C with a 700W microwave, keep the temperature for 8 minutes, cool the temperature to 45°C, soak for 1h , filter, combine twice filtrate concentration and drying to obtain Dendrobium officinale extract;
所述铁皮石斛和酶化提取液的质量比为1:5;所述搅拌的速率为80r/min;The mass ratio of the Dendrobium officinale and the enzymatic extract is 1:5; the stirring speed is 80r/min;
C黄精提取C Polygonatum Extract
将黄精捣碎,加入A中酶化提取液混合搅拌10min,在零下20℃下冷冻5h,取出用碎冰机粉碎,将粉碎物加热至5℃,用600W的超声波处理20min,在40℃下静置50min过滤,收集滤液,将滤液浓缩烘干即得黄精提取物;Mash the Polygonatum glutinosa, add the enzymatic extract in A, mix and stir for 10 minutes, freeze at -20°C for 5 hours, take out and pulverize with an ice crusher, heat the pulverized material to 5°C, use 600W ultrasonic treatment for 20min, at 40°C Leave standstill for 50min to filter, collect the filtrate, concentrate and dry the filtrate to obtain Polygonatum chinensis extract;
所述黄精和酶化提取液的质量比为1:10;The mass ratio of the Polygonatum chinensis and the enzymatic extract is 1:10;
(2)原料调制(2) Raw material preparation
将上一步骤中的提取黄精剩的滤渣和提取铁皮石斛剩的滤渣按照质量比23:8混合,加入糯玉米粉,调节含水量为75%,接种乳杆菌,发酵18h,搅拌均匀即可;Mix the remaining filter residue from the extraction of Polygonatum chinensis in the previous step and the residue from the extraction of Dendrobium officinale according to a mass ratio of 23:8, add waxy corn flour, adjust the water content to 75%, inoculate with Lactobacillus, ferment for 18 hours, and stir evenly;
所述糯玉米粉和混合滤渣的质量比为1:5;所述乳杆菌的用量为发酵底物质量的2%;所述发酵温度为25℃;The mass ratio of the waxy corn flour and the mixed filter residue is 1:5; the consumption of the lactobacillus is 2% of the mass of the fermentation substrate; the fermentation temperature is 25°C;
(3)原料蒸晒(3) Steaming and drying of raw materials
将步骤(2)中物料在90℃下蒸80min,制作成直径1cm的圆球,放置到太阳下暴晒2天,然后收集放回蒸笼中蒸2h,暴晒1天,再次蒸2h晾凉即可;Steam the material in step (2) at 90°C for 80min to make a ball with a diameter of 1cm, put it in the sun for 2 days, then collect it and put it back in the steamer for 2h, expose it for 1 day, steam again for 2h and let it cool. ;
(4)原料成型(4) Raw material molding
将步骤(1)中铁皮石斛提取物和黄精提取物按照质量1:3混合,稀释3倍,加入海藻酸钠、果胶混合均匀得到强化液,将步骤(3)制作的圆球浸没到强化液中1min,捞出沥干水分,再次浸没到强化液中2min,捞出在40℃下减压干燥即可得到所述咀嚼丸;In the step (1), the Dendrobium officinale extract and the Polygonatum chinensis extract are mixed according to the mass 1:3, diluted 3 times, sodium alginate and pectin are added and mixed evenly to obtain a strengthening liquid, and the spheres made in step (3) are immersed in the strengthening solution. In the liquid for 1 min, take out and drain the water, immerse in the strengthening liquid again for 2 min, take out and dry under reduced pressure at 40°C to obtain the chewable pills;
所述海藻酸钠的用量为黄精提取物质量的25%;所述果胶的用量为黄精提取物质量的13%。The dosage of the sodium alginate is 25% of the mass of the Polygonatum chinensis extract; the dosage of the pectin is 13% of the mass of the Polygonatum chinensis extract.
实施例2Example 2
一种添加铁皮石斛的黄精咀嚼丸的制作方法,包括如下步骤:A preparation method of Huangjing chewable pills with Dendrobium officinale, comprising the steps:
(1)原料提取(1) Raw material extraction
A制备酸性酶化提取液A Preparation of acid enzymatic extract
以质量份计,将30份杨梅、13份广枣、17份山楂、7份鱼粉、15份麦芽混合粉碎磨浆,在140℃下灭菌4h,将混合物恢复至常温,接种酿酒酵母菌,在31℃下密封发酵8h,接种醋酸杆菌,在33℃下发酵80h,加水稀释3倍,继续发酵23h,稀释5倍后抽滤即得所述酶化提取液;In parts by mass, 30 parts of bayberry, 13 parts of jujube, 17 parts of hawthorn, 7 parts of fish meal, and 15 parts of malt were mixed, crushed and refined, sterilized at 140 ° C for 4 hours, the mixture was returned to normal temperature, and inoculated with Saccharomyces cerevisiae, Sealed and fermented at 31°C for 8h, inoculated with Acetobacter, fermented at 33°C for 80h, diluted 3 times with water, continued to ferment for 23h, diluted 5 times and filtered to obtain the enzymatic extract;
所述麦芽的制作方法为:将大麦和小麦按照质量比1:0.33混合,用质量分数0.0008%的硒酸钠溶液混合,浸泡8h,调节含水量为90%,在18℃下催芽至胚芽鞘突破种皮5mm即可;所述酿酒酵母的接种量为混合物质量的9%;所述醋酸杆菌的接种量为混合物质量的8%;The preparation method of the malt is as follows: mixing barley and wheat according to a mass ratio of 1:0.33, mixing with a sodium selenate solution with a mass fraction of 0.0008%, soaking for 8 hours, adjusting the water content to 90%, and accelerating the germination to the coleoptile at 18° C. Break through the seed coat by 5mm; the inoculation amount of the Saccharomyces cerevisiae is 9% of the mass of the mixture; the inoculum of the Acetobacter is 8% of the mass of the mixture;
B铁皮石斛提取B Dendrobium officinale Extract
将新鲜铁皮石斛的粉碎,加入上述A制得的酶化提取液混合在38℃下浸泡30min,自然冷却至室温,用700W的微波加热至48℃,保温18min,搅拌50min,然后抽滤收集滤液;将滤渣与其质量10倍的食用乙醇混合,用900W的微波加热至60℃,浸泡18min,自然降温至室温,用900W的微波加热至68℃,保温10min,将温度降温至50℃,浸泡3h,过滤,合并两次滤液浓缩烘干得到铁皮石斛提取物;Pulverize fresh Dendrobium officinale, add the enzymatic extract prepared from above A, mix and soak at 38°C for 30min, naturally cool to room temperature, heat to 48°C with 700W microwave, keep warm for 18min, stir for 50min, and then collect the filtrate by suction filtration ; Mix the filter residue with 10 times its mass of edible ethanol, heat it to 60°C with a 900W microwave, soak it for 18 minutes, cool it down to room temperature naturally, heat it to 68°C with a 900W microwave, keep it warm for 10 minutes, cool the temperature to 50°C, soak for 3h , filter, combine twice filtrate concentration and drying to obtain Dendrobium officinale extract;
所述铁皮石斛和酶化提取液的质量比为1:8;所述搅拌的速率为100r/min;The mass ratio of the Dendrobium officinale and the enzymatic extraction solution is 1:8; the speed of the stirring is 100r/min;
C黄精提取C Polygonatum Extract
将黄精捣碎,加入A中酶化提取液混合搅拌15min,在零下25℃下冷冻9h,取出用碎冰机粉碎,将粉碎物加热至10℃,用700W的超声波处理25min,在43℃下静置80min过滤,收集滤液,将滤液浓缩烘干即得黄精提取物;Mash the Polygonatum glutinosa, add the enzymatic extract in A, mix and stir for 15 minutes, freeze at minus 25 °C for 9 hours, take out and pulverize with an ice crusher, heat the pulverized material to 10 °C, use 700W ultrasonic treatment for 25 minutes, at 43 °C Leave standstill for 80min to filter, collect the filtrate, concentrate and dry the filtrate to obtain Polygonatum chinensis extract;
所述黄精和酶化提取液的质量比为1:13;The mass ratio of described Polygonatum chinensis and the enzymatic extract is 1:13;
(2)原料调制(2) Raw material preparation
将上一步骤中的提取黄精剩的滤渣和提取铁皮石斛剩的滤渣按照质量比23:11混合,加入糯玉米粉,调节含水量为75-88%,接种乳杆菌,发酵22h,搅拌均匀即可;Mix the remaining filter residue from the extraction of Polygonatum chinensis in the previous step and the remaining filter residue from the extraction of Dendrobium officinale according to a mass ratio of 23:11, add waxy corn flour, adjust the water content to 75-88%, inoculate with Lactobacillus, ferment for 22 hours, and stir evenly. Can;
所述糯玉米粉和混合滤渣的质量比为1:7;所述乳杆菌的用量为发酵底物质量的4%;所述发酵温度为28℃;The mass ratio of the waxy corn flour and the mixed filter residue is 1:7; the consumption of the lactobacillus is 4% of the mass of the fermentation substrate; the fermentation temperature is 28°C;
(3)原料蒸晒(3) Steaming and drying of raw materials
将步骤(2)中物料在95℃下蒸100min,制作成直径1.5cm的圆球,放置到太阳下暴晒3天,然后收集放回蒸笼中蒸3h,暴晒2天,再次蒸3h晾凉即可;The material in step (2) was steamed at 95°C for 100min, made into a ball with a diameter of 1.5cm, placed in the sun for 3 days, then collected and put back in the steamer to steam for 3h, exposed to the sun for 2 days, steamed again for 3h and allowed to cool. Can;
(4)原料成型(4) Raw material molding
将步骤(1)中铁皮石斛提取物和黄精提取物按照质量1:3混合,稀释5倍,加入海藻酸钠、果胶混合均匀得到强化液,将步骤(3)制作的圆球浸没到强化液中2min,捞出沥干水分,再次浸没到强化液中3min,捞出在45℃下减压干燥即可得到所述咀嚼丸;In the step (1), the Dendrobium officinale extract and the Polygonatum chinensis extract are mixed according to the mass 1:3, diluted 5 times, sodium alginate and pectin are added and mixed evenly to obtain a strengthening solution, and the spheres made in step (3) are immersed in the strengthening solution. In the liquid for 2 minutes, take out and drain the water, immerse in the strengthening liquid again for 3 minutes, take out and dry under reduced pressure at 45°C to obtain the chewable pills;
所述海藻酸钠的用量为黄精提取物质量的30%;所述果胶的用量为黄精提取物质量的18%。The dosage of the sodium alginate is 30% of the mass of the Polygonatum chinensis extract; the dosage of the pectin is 18% of the mass of the Polygoni chinensis extract.
实施例3Example 3
一种添加铁皮石斛的黄精咀嚼丸的制作方法,包括如下步骤:A preparation method of Huangjing chewable pills with Dendrobium officinale, comprising the steps:
(1)原料提取(1) Raw material extraction
A制备酸性酶化提取液A Preparation of acid enzymatic extract
以质量份计,将23份杨梅、11份广枣、15份山楂、5份鱼粉、13份麦芽混合粉碎磨浆,在130℃下灭菌3h,将混合物恢复至常温,接种酿酒酵母菌,在31℃下密封发酵5h,接种醋酸杆菌,在33℃下发酵70h,加水稀释3倍,继续发酵10h,稀释5倍后抽滤即得所述酶化提取液;In parts by mass, 23 parts of bayberry, 11 parts of jujube, 15 parts of hawthorn, 5 parts of fish meal, and 13 parts of malt were mixed, crushed and refined, sterilized at 130 ° C for 3 hours, the mixture was returned to normal temperature, and inoculated with Saccharomyces cerevisiae, Sealed and fermented at 31°C for 5h, inoculated with Acetobacter, fermented at 33°C for 70h, diluted 3 times with water, continued to ferment for 10h, diluted 5 times and filtered to obtain the enzymatic extract;
所述麦芽的制作方法为:将大麦和小麦按照质量比1:0.33混合,用质量分数0.0008%的硒酸钠溶液混合,浸泡5h,调节含水量为90%,在15℃下催芽至胚芽鞘突破种皮5mm即可;所述酿酒酵母的接种量为混合物质量的9%;所述醋酸杆菌的接种量为混合物质量的3%;The preparation method of the malt is as follows: mixing barley and wheat according to a mass ratio of 1:0.33, mixing with a sodium selenate solution with a mass fraction of 0.0008%, soaking for 5 hours, adjusting the water content to 90%, and accelerating germination to coleoptiles at 15° C. Break through the seed coat by 5 mm; the inoculation amount of the Saccharomyces cerevisiae is 9% of the mass of the mixture; the inoculum of the Acetobacter is 3% of the mass of the mixture;
B铁皮石斛提取B Dendrobium officinale Extract
将新鲜铁皮石斛的粉碎,加入上述A制得的酶化提取液混合在38℃下浸泡20min,自然冷却至室温,用700W的微波加热至48℃,保温13min,搅拌30min,然后抽滤收集滤液;将滤渣与其质量10倍的食用乙醇混合,用900W的微波加热至55℃,浸泡18min,自然降温至室温,用900W的微波加热至65℃,保温10min,将温度降温至45℃,浸泡3h,过滤,合并两次滤液浓缩烘干得到铁皮石斛提取物;Pulverize fresh Dendrobium officinale, add the enzymatic extract prepared by A above, mix and soak at 38°C for 20min, naturally cool to room temperature, heat to 48°C with 700W microwave, keep warm for 13min, stir for 30min, and then collect the filtrate by suction filtration ; Mix the filter residue with 10 times its mass of edible ethanol, heat it to 55°C with a 900W microwave, soak it for 18 minutes, cool it down to room temperature naturally, heat it to 65°C with a 900W microwave, keep it warm for 10 minutes, cool it down to 45°C, soak for 3 hours , filter, combine twice filtrate concentration and drying to obtain Dendrobium officinale extract;
所述铁皮石斛和酶化提取液的质量比为1:5;所述搅拌的速率为100r/min;The mass ratio of the Dendrobium officinale and the enzymatic extract is 1:5; the stirring speed is 100r/min;
C黄精提取C Polygonatum Extract
将黄精捣碎,加入A中酶化提取液混合搅拌15min,在零下20℃下冷冻9h,取出用碎冰机粉碎,将粉碎物加热至10℃,用600W的超声波处理25min,在40℃下静置80min过滤,收集滤液,将滤液浓缩烘干即得黄精提取物;Mash the Polygonatum glutinosa, add the enzymatic extract in A, mix and stir for 15 minutes, freeze at -20°C for 9 hours, take out and pulverize with an ice crusher, heat the pulverized material to 10°C, use 600W ultrasonic treatment for 25min, at 40°C Leave standstill for 80min to filter, collect the filtrate, concentrate and dry the filtrate to obtain Polygonatum chinensis extract;
所述黄精和酶化提取液的质量比为1:13;The mass ratio of the Polygonatum chinensis and the enzymatic extract is 1:13;
(2)原料调制(2) Raw material preparation
将上一步骤中的提取黄精剩的滤渣和提取铁皮石斛剩的滤渣按照质量比23:11混合,加入糯玉米粉,调节含水量为75%,接种乳杆菌,发酵22h,搅拌均匀即可;Mix the remaining filter residue from the extraction of Polygonatum chinensis in the previous step and the remaining filter residue from the extraction of Dendrobium officinale according to a mass ratio of 23:11, add waxy corn flour, adjust the water content to 75%, inoculate with Lactobacillus, ferment for 22 hours, and stir evenly;
所述糯玉米粉和混合滤渣的质量比为1:7;所述乳杆菌的用量为发酵底物质量的2%;所述发酵温度为28℃;The mass ratio of the waxy corn flour and the mixed filter residue is 1:7; the consumption of the lactobacillus is 2% of the mass of the fermentation substrate; the fermentation temperature is 28°C;
(3)原料蒸晒(3) Steaming and drying of raw materials
将步骤(2)中物料在95℃下蒸80min,制作成直径1.5cm的圆球,放置到太阳下暴晒2天,然后收集放回蒸笼中蒸3h,暴晒1天,再次蒸3h晾凉即可;The material in step (2) was steamed at 95°C for 80min, made into a ball with a diameter of 1.5cm, placed in the sun for 2 days, then collected and put back in the steamer to steam for 3h, exposed to the sun for 1 day, steamed again for 3h and allowed to cool. Can;
(4)原料成型(4) Raw material molding
将步骤(1)中铁皮石斛提取物和黄精提取物按照质量1:3混合,稀释5倍,加入海藻酸钠、果胶混合均匀得到强化液,将步骤(3)制作的圆球浸没到强化液中1min,捞出沥干水分,再次浸没到强化液中3min,捞出在40℃下减压干燥即可得到所述咀嚼丸;In the step (1), the Dendrobium officinale extract and the Polygonatum chinensis extract are mixed according to the mass 1:3, diluted 5 times, sodium alginate and pectin are added and mixed evenly to obtain a strengthening solution, and the spheres made in step (3) are immersed in the strengthening solution. In the liquid for 1min, take out and drain the water, immerse in the strengthening liquid again for 3min, take out and dry under reduced pressure at 40°C to obtain the chewable pills;
所述海藻酸钠的用量为黄精提取物质量的30%;所述果胶的用量为黄精提取物质量的18%。The dosage of the sodium alginate is 30% of the mass of the Polygonatum chinensis extract; the dosage of the pectin is 18% of the mass of the Polygoni chinensis extract.
对比例设置:Contrast ratio settings:
试验例1本发明小鼠肥胖实验Test Example 1 The mouse obesity experiment of the present invention
选择体重20±1.3g的昆明小鼠,性别比为1:1,正常组10只,造模组90只。正常组使用常规全营养饲料喂养,造模组在饲料中添加15%的牛油,连续喂养两周,按照公式Lee's指数=体重1/3(g)×1000/体长(cm),造模组小鼠指数比正常组小鼠高8%,达到肥胖模型。开始正式实验,将造模组分为实施例1-3组、对比1-5组、对照组。实施例1-3组在喂养饲料中对应添加实施例1-3制作的咀嚼丸,用量为饲料用量的8%;对比例1-5组在喂养饲料中对应添加对比例1-5制作的咀嚼丸,用量为饲料用量的8%;对照组继续饲喂高脂饲料;连续饲喂5周。Select Kunming mice weighing 20±1.3g with a sex ratio of 1:1, 10 in the normal group and 90 in the modeling group. The normal group was fed with conventional full-nutrition feed, and the modeling group was fed with 15% tallow for two consecutive weeks. The index of the mice in the modeling group was 8% higher than that in the normal group, reaching the obesity model. The formal experiment was started, and the modeling groups were divided into Example 1-3 groups, Comparative 1-5 groups, and control groups. The chewing pills prepared in Example 1-3 were added to the feeding feeds in Example 1-3 groups, and the dosage was 8% of the amount of the feed; the chewing pills prepared in Comparative Examples 1-5 were added to the feeding feeds in groups of Comparative Examples 1-5. Pills, the dosage was 8% of the feed amount; the control group continued to be fed with high-fat feed; fed continuously for 5 weeks.
用合肥莱尔生物科技有限公司血清瘦素试剂盒检测血清瘦素水平。Serum leptin levels were detected with serum leptin kit from Hefei Laier Biotechnology Co., Ltd.
由表可以看出,实施例1-3中小鼠的瘦素最高,显然本发明制作的咀嚼丸有效抑制肥胖,对于机体的脂质代谢有良好的促进作用,适宜减肥人群使用。It can be seen from the table that the mice in Examples 1-3 have the highest leptin. Obviously, the chewable pills made by the present invention can effectively inhibit obesity, have a good promoting effect on the lipid metabolism of the body, and are suitable for people who lose weight.
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