CN114668702B - No-wash quick-drying hand sanitizer and preparation method thereof - Google Patents

Abstract

The invention discloses a washing-free quick-drying hand sanitizer and a preparation method thereof, and belongs to the technical field of disinfection. The disinfectant consists of the following components in percentage by weight: 73-82% of ethanol, 0.1-0.16% of hydrogen peroxide, 0.5-2.0% of glycerol, 0.1-0.5% of synergistic skin care component and the balance of deionized water, wherein the synergistic skin care component comprises honey, betaine and silicone oil according to the weight ratio of 1:2-6: 1-5. The washing-free quick-drying hand disinfectant can kill enteropathogenic bacteria, suppurative coccus, pathogenic microzyme, common bacteria infected by hospital and the like, and can inactivate viruses under the hand cleaning condition. Meanwhile, the disinfectant is basically non-irritating and is not easy to cause anaphylactic reaction.

Description

No-wash quick-drying hand sanitizer and preparation method thereof

Technical Field

The invention relates to the technical field of disinfection, in particular to a washing-free quick-drying hand disinfectant and a preparation method thereof.

Background

Hand washing has been a major concern in life, and the chances of contacting other people or things have been greatly increased from previous hand washing before meals to current public transportation, and the chances of transmitting diseases through the hands have been increased. The hand cleaning and sterilizing mode also has better and better cleaning effect from the previous soap and soap hand washing to the current hand washing liquid hand washing, and is more convenient to use. Later, no-wash liquid appears, and no-wash liquid does not need to use towels, water sources, soaps and the like, is convenient to carry and use and is accepted by people gradually. The main component of the hand-free quick-drying hand-sterilizing liquid is usually ethanol, such as ethanol, hydrogen peroxide and glycerol, which is a classical hand-free quick-drying hand-sterilizing liquid.

In the prior art, high-concentration ethanol (such as about 75%) can be used for preparing a washing-free quick-drying hand disinfectant, for example, the patent with the application number of CN202010380388.8 discloses a preparation method of a polysaccharide quick-drying hand disinfectant, and the disinfectant comprises the following components in parts by weight: 80ml of ethanol; hydrogen peroxide 0.4ml; 0.1g of glucan polysaccharide quaternary ammonium salt; glycerol 1.5ml; purified water 18ml. In the patent, the quaternary ammonium salt of the glucan is used as a disinfectant and a skin care component, and the quaternary ammonium glucan is applied to a hand disinfectant, so that the disinfectant performance can be improved, and the effects of preventing bacteria and viruses from diffusing, protecting skin and the like can be achieved.

The applicant found during use that: high concentrations of ethanol are strongly irritating and, if used directly, do not give a good user experience. Therefore, skin care ingredients are added to the prior art. The aforementioned patent incorporates a quaternary ammonium salt of dextran as a skin care ingredient, but the skin care effect of the quaternary ammonium salt of dextran is generally costly. In addition, there are a large number of people allergic to alcohol in the population, and high concentration alcohol is liable to cause allergic reactions.

Disclosure of Invention

In order to solve the problems, the embodiment of the invention provides a washing-free quick-drying hand disinfectant which has the advantages of better sterilization effect, low irritation, difficult anaphylactic reaction and the like. The technical scheme is as follows:

on one hand, the embodiment of the invention provides a washing-free quick-drying hand disinfectant, which comprises the following components in percentage by weight: 73-82% of ethanol, 0.1-0.16% of hydrogen peroxide, 0.5-2.0% of glycerol, 0.1-0.5% of synergistic skin care component and the balance of deionized water, wherein the synergistic skin care component comprises honey, betaine and silicone oil according to the weight ratio of 1:2-6: 1-5.

Preferably, the washing-free quick-drying hand sanitizer provided by the embodiment of the invention comprises the following components in percentage by weight: 75% of ethanol, 0.1% of hydrogen peroxide, 1.0% of glycerol, 0.4% of synergistic skin care component and the balance of deionized water; wherein the synergistic skin care component comprises honey, betaine and silicone oil according to the weight ratio of 1:4:2, compounding.

On the other hand, the embodiment of the invention also provides a preparation method of the washing-free quick-drying hand disinfectant, which comprises the following steps:

(1) Dissolving Mel in water, and mixing to obtain Mel water.

(2) And (3) dissolving the silicone oil and the glycerol in part (5-20%) of ethanol, and uniformly mixing to obtain an ethanol solution.

(3) Mixing Mel water, ethanol solution, hydrogen peroxide, betaine and the rest ethanol, stirring, and standing to obtain the final product.

The preparation method is convenient for dissolving various substances, does not generate foam in the stirring process, and is easy to produce.

The disinfectant provided by the invention has the following beneficial effects:

(1) The washing-free quick-drying hand disinfectant can kill enteropathogenic bacteria, suppurative coccus, pathogenic saccharomycetes and common bacteria of nosocomial infection.

(2) The washing-free quick-drying hand disinfectant can inactivate viruses under the hand cleaning condition. The synergistic skin care component can promote the sterilization effect under the hand cleaning condition.

(3) The washing-free quick-drying hand disinfectant is basically free of stimulation, and is good in user experience.

(4) The washing-free quick-drying hand disinfectant is not easy to cause anaphylactic reaction.

(5) The synergistic skin care component is a common component of the cleaning product, and has low cost.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention will be described in further detail below.

Example 1

The embodiment I discloses a washing-free quick-drying hand disinfectant, which consists of the following components: 7.5kg of ethanol, 10g of hydrogen peroxide, 100g of glycerin, 5.7g of honey, 22.9g of betaine, 11.4g of silicone oil and 2.35kg of deionized water.

Example two

The second embodiment discloses a washing-free quick-drying hand disinfectant, which consists of the following components: 8.0kg of ethanol, 15g of hydrogen peroxide, 85g of glycerin, 5g of honey, 15g of betaine, 10g of silicone oil and 18.7kg of deionized water.

Example III

The third embodiment discloses a washing-free quick-drying hand disinfectant, which consists of the following components: 7.8kg of ethanol, 10g of hydrogen peroxide, 140g of glycerol, 10g of honey, 20g of betaine, 20g of silicone oil and 2.00kg of deionized water.

Verification examples, the disinfection solution provided in example 1 was used for all experiments.

1. Sterilization test

1.1. Test strain: coli 8099, staphylococcus aureus ATC6538 and pseudomonas aeruginosa ATCC15442, all provided by the food safety strain collection center of the food safety engineering technology research and development center of guangdong province, were taken and tested as 5 th generation fresh slant cultures.

1.2. Neutralizing agent: 0.03mol/L PBS of 3% Tween 80+0.3% lecithin+0.1% sodium thiosulfate.

1.3. Culture medium: sand agar medium.

1.4. Organic interfering substances: 3% BSA solution.

1.5. The method comprises the following steps: according to the disinfection technical Specification (2002 edition) 2.1.1.5, 2.1.1.7. Neutralization test: the test bacteria are Escherichia coli, the sterilization time is 0.5min, the test environment temperature is 19-21 ℃, the relative humidity is 45-60%, and the test is repeated for 3 times. Sterilization test: sterilizing with disinfectant, and repeating the test for 3 times at 19-21deg.C and 45-60% relative humidity. The culture temperature was 37 ℃.

1.6. Positive control: the test procedure was followed using sterile dilutions instead of test solutions.

1.7. Coli neutralizer identification assay, the results are shown in table 1:

TABLE 1

As can be seen from table 1: the average recovery bacteria number of the 1 st group is 0CFU/mL, the average recovery bacteria number of the 2 nd group is 213CFU/mL, the error rates of the 3 rd, 4 th and 5 th groups are 2.72%, 2.40%, 3.28% respectively, and the 6 th group is aseptically grown. Therefore, the disinfectant has a killing effect on the escherichia coli, and the disinfectant and the neutralization products have no adverse effect on the escherichia coli.

1.8. Test for killing of test bacteria: the test is repeated for 3 times at the test environment temperature of 19-21 ℃, the sterilization pair number of the sterilizing liquid respectively acts for 0.5min, 1min and 1.5min on escherichia coli, staphylococcus aureus and pseudomonas aeruginosa is more than 5.00, and the results are shown in tables 2-4:

TABLE 2

TABLE 3 Table 3

TABLE 4 Table 4

2. Fungus killing test (suspension method)

2.1. Test strain: candida albicans (ATCC 10231), supplied by the food safety strain collection center of the food safety engineering research and development center of the cantonese province, was taken and tested on a 5 th generation fresh slant culture.

2.2. Neutralizing agent: 0.03mol/L PBS of 3% Tween 80+0.3% lecithin+0.1% sodium thiosulfate.

2.3. Culture medium: sand agar medium.

2.4. Organic interfering substances: 3% BSA solution.

2.5. The method comprises the following steps: according to the disinfection technical Specification (2002 edition) 2.1.1.5, 2.1.1.9. Neutralization test: the sterilization time is 0.5min, the test environment temperature is 19-21 ℃, the relative humidity is 45-60%, and the test is repeated for 3 times. Sterilization test: sterilizing with disinfectant, and repeating the test for 3 times at 19-21deg.C and 45-60% relative humidity. The culture temperature was 37 ℃.

2.6. Positive control: the test procedure was followed using sterile dilutions instead of test solutions. The results are shown in Table 5:

TABLE 5

As can be seen from table 5: the average recovery bacteria number of the 1 st group is 0CFU/mL, the average recovery bacteria number of the 2 nd group is 470CFU/mL, the error rate of the colony numbers of the 3 rd group, the 4 th group and the 5 th group is 1.11%, 1.50%, 0.80% respectively, and the 6 th group is aseptically grown.

2.7. Killing effect on experimental bacteria: the experimental environment temperature is 19-21 ℃, the experiment is repeated for 3 times, the sterilization liquid acts for 0.5min, 1min and 1.5min, the killing logarithmic value of candida albicans is more than 5.00, and the results are shown in table 6:

TABLE 6

3. Hand disinfection field test (sanitary hand)

3.1. Neutralizing agent: 0.03mol/L PBS of 3% Tween 80+0.3% lecithin+0.1% sodium thiosulfate.

3.2. A diluent: 0.03mol/L phosphate buffer, pH7.2, of 0.1% Tween 80.

3.3. Culture medium: tryptone soy agar medium.

3.4. The method comprises the following steps: according to the disinfection technical Specification (2002 edition) 2.1.2.6. Disinfection test: the disinfectant is used as a sample, the wiping disinfection effect is carried out for 1min, the test environment temperature is 19-21 ℃, and the relative humidity is 45-60%. A subject: the left hand of 30 volunteers (sanitary hands) was sampled before disinfection as a positive control group, and the samples were applied to the right hand of the volunteers for 1min and then the samples were used as a test group. The sampling method comprises the following steps: the aseptic swab is wetted in a sampling liquid test tube, the positive control group and the disinfection test group are smeared and sampled, and the sampling end of the swab is sheared into 10mL of sampling liquid in an aseptic mode and eluted. Negative control: taking the sampling liquid and the dilution liquid of the same batch for direct culture. The results are shown in Table 7:

TABLE 7

As can be seen from the results of Table 7, the average kill log value is > 1.81, meets the requirements of the "sterilization Specification", and is > 1.00.

4. Hand disinfection field test (surgical hand)

4.1. Neutralizing agent: 0.03mol/L PBS of 3% Tween 80+0.3% lecithin+0.1% sodium thiosulfate.

4.2. A diluent: 0.03mol/L phosphate buffer, pH7.2, of 0.1% Tween 80.

4.3. Culture medium: tryptone soy agar medium.

4.4. The method comprises the following steps: according to the disinfection technical Specification (2002 edition) 2.1.2.6. Disinfection test: the disinfectant is used as a sample, the wiping and disinfection effects are carried out for 3min, the test environment temperature is 19-21 ℃, and the relative humidity is 45-60%. A subject: the left hand of 30 volunteers (surgical hands) was sampled before sterilization as a positive control group, and the samples were applied to the right hand of the volunteers for 3min and then sampled as a test group. The sampling method comprises the following steps: the aseptic swab is wetted in a sampling liquid test tube, the positive control group and the disinfection test group are smeared and sampled, and the sampling end of the swab is sheared into 10mL of sampling liquid in an aseptic mode and eluted. Negative control: taking the sampling liquid and the dilution liquid of the same batch for direct culture. The results are shown in Table 8:

TABLE 8

As can be seen from the results of Table 8, the average kill log value is > 1.87, meets the requirements of the "sterilization Specification", and is > 1.00.

5. Multiple complete skin irritation test

5.1 animal information:

variety: guinea pigs, dunkin Hartley 3;

the source is as follows: all-round-jia-xing biotechnology limited company in the Wuhan market;

grade: a normal stage;

animal pass number: no.42010000005104;

gender: a female;

body weight range: 300-400g;

feed: maintaining feed for normal guinea pigs;

animal room environment: temperature 18-29 ℃, relative humidity: 40% -70%;

test animal use license number: SYXK 2017-0096.

5.2 main reagents: 0.9% NaCl injection.

5.3 method: according to the disinfection technical Specification (2002 edition) 2.3.3.3.3. The test was performed directly using 0.5mL of disinfectant. The process is as follows: the hairiness on both sides of the back spine of guinea pigs was removed 24 hours prior to the test and the skin was not damaged. The dehairing range is about 3cm by 3cm on the left and right. The next day 0.5mL of the test article was applied onto 2-4 gauze layers with an area of 2.5cm x 2.5cm and applied to the skin surface of one side, then covered with a layer of non-irritating plastic film and fixed with non-irritating adhesive tape. The other side was coated with 0.5ml of 0.9% sodium chloride injection as a control, and after 4 hours of coating, the mixture was washed with warm water to remove the residue. The application was once daily for 14 days. The residual test substance was washed with water or a suitable solvent without irritation 4 hours after each application of the test substance, and the animal skin was observed for erythema and edema at 24 hours after application of the test substance, and scored according to the scoring criteria for skin irritation response. The results are shown in Table 9:

TABLE 9

From the results in Table 9, it can be seen that the disinfectant in this example is non-irritating.

6. Acute toxicity test

6.1. Standard: according to the disinfection technical Specification (2002 edition).

6.2. Detection environment: SPF-class animal house with room temperature of 20-26 deg.C and humidity of 40-70%.

6.3. Test animals: 20 SPF-class Kunming mice, male and female halves, body weight 18-22 g, experimental animals and feed were purchased from Si Bei Fu (Beijing) Biotechnology Co., ltd.

6.4. The test steps are as follows: in the pre-test, no death of the sample preparation liquid at the dosage of 5000mg/kg body weight is found, so that the main test adopts one maximum test, and the test object is measured to be 5000mg/kg body weight. The test solution was administered orally by gavage in an amount of 0.1mL/10g body weight, and the animals fasted for 4 hours before gavage and were free to drink water. Normal diet was given 1h after gastric lavage. The experimental observation is carried out for 14 days, the poisoning performance, death number and death time of the animals are observed after the infection, autopsy is carried out on the dead animals and the sacrificed animals at the expiration of the observation, and whether abnormal tissues or organs exist or not is observed with naked eyes. The results are shown in Table 10:

table 10

As can be seen from table 10: all animals tested did not show obvious poisoning symptoms and death during the 14 day observation period. So that acute oral toxicity LD of mice ₅₀ > 5000mg/kg body weight.

Comparative example one

The disinfectant of the first comparative example consists of the following components: 7.5kg of ethanol, 10g of hydrogen peroxide, 100g of glycerol and 2.39kg of deionized water.

Comparative example two

The disinfectant of the second comparative example consists of the following components: 7.5kg of ethanol, 10g of hydrogen peroxide, 100g of glycerin, 5.7g of honey and 2.39kg of deionized water.

Comparative example three

The disinfectant of the third comparative example consists of the following components: 7.5kg of ethanol, 10g of hydrogen peroxide, 100g of glycerol, 11.4g of silicone oil and 2.38kg of deionized water.

Comparative example four

The disinfectant of the fourth comparative example consists of the following components: 7.5kg of ethanol, 10g of hydrogen peroxide, 100g of glycerol, 22.9g of betaine and 2.37kg of deionized water.

The disinfection test, the process is seen in the disinfection solution hand disinfection field test (hygienic hand) and the disinfection solution hand disinfection field test (surgical hand) described above, and the results are shown in table 11:

TABLE 11

As can be seen from Table 11, the addition of honey, betaine and silicone oil alone did not substantially improve the sterilization effect, whereas the addition of honey, betaine and silicone oil in a certain ratio significantly improved the sterilization effect.

Irritation test procedure see the previous multiple complete skin irritation test, recording the number of days when erythema and edema began to appear, calculating the average, and the results are shown in table 12:

table 12

As can be seen from table 12: the combination of ethanol, hydrogen peroxide and glycerol had a pronounced irritation, and the symptoms were essentially pronounced on day 2, with the addition of honey, betaine and silicone oil alone slightly improved the irritation relative to comparative example 1, but the irritation improvement was not pronounced with a pronounced response around 1 week. The disinfectant provided by the invention has no obvious symptoms in the test time of 14 days.

Allergy test: looking for 25 volunteers (male 17, female 8, average age 26.32 years), skin of the volunteers was allergic to alcohol or hydrogen peroxide, six kinds of sterilizing solutions were applied to left or right hands (examples 1-2 and comparative examples 1-4, respectively, the latter were the same), the application time was 5 minutes, the application area was more than 2cm x 2cm, the application was maintained for 30 minutes, the state of the applied area was observed, if allergic symptoms such as blisters, red swelling or itching were found, it was judged to be allergic, and after the time was satisfied or obvious symptoms were found, the results were shown in table 13:

TABLE 13

As can be seen from table 13: the addition of honey, betaine and silicone oil alone showed no substantial improvement in allergy compared with comparative example 1, whereas with the disinfectant of the present invention, the number of allergic persons was significantly reduced, and the probability of causing allergic reactions was significantly reduced.

Sensory evaluation test: searching for 37 volunteers (male 16, female 21, average age 25.08 years), and applying six disinfectant solutions to left or right hand for 3min with application area greater than 2cm x 2cm. Sensory evaluation was performed on the scale of 1 score for strong stimulation, 2 score for general stimulation, 3 score for weak stimulation, and 4 score for no stimulation, and the results are shown in table 14:

TABLE 14

From table 14, it can be seen that: the combination of ethanol, hydrogen peroxide and glycerol had a pronounced irritation, and the addition of honey, betaine and silicone oil alone had a slightly reduced irritation, but very insignificant effect, relative to comparative example 1. When the disinfectant disclosed by the invention is used, the ordinary consumer basically cannot feel the irritation.

The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.

Claims (3)

1. The washing-free quick-drying hand disinfectant is characterized by comprising the following components in percentage by weight: 73-82% of ethanol, 0.1-0.16% of hydrogen peroxide, 0.5-2.0% of glycerol, 0.1-0.5% of synergistic skin care component and the balance of deionized water, wherein the synergistic skin care component comprises honey, betaine and silicone oil according to the weight ratio of 1:2-6: 1-5.

2. The no-clean quick-drying hand sanitizer of claim 1, wherein the disinfectant is composed of the following components in percentage by weight: 75% of ethanol, 0.1% of hydrogen peroxide, 1.0% of glycerol, 0.4% of synergistic skin care component and the balance of deionized water, wherein the synergistic skin care component comprises honey, betaine and silicone oil according to the weight ratio of 1:4:2, compounding.

3. A method for preparing a leave-in quick-drying hand sanitizer according to any of claims 1-2, comprising the steps of:

(1) Dissolving Mel in water, and mixing to obtain Mel water;

(2) Dissolving silicone oil and glycerol in part of ethanol, and uniformly mixing to obtain an ethanol solution;

(3) Mixing Mel water, ethanol solution, hydrogen peroxide, betaine and the rest ethanol, stirring, and standing to obtain the final product.